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Multi-trait selection for mean performance and stability in purple-fleshed sweet potato 紫瓤甘薯平均性能和稳定性的多性状选择
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-25 DOI: 10.1016/j.sajb.2024.09.048
Murilo Henrique Souza Leal , André Dutra Silva Júnior , Julia Roberta Sanches de Pieri , Bruno da Rocha Toroco , Guilherme José Almeida Oliveira , João Lucas Pires Leal , Tiago Olivoto , Edgard Henrique Costa Silva , André Ricardo Zeist
Few purple-fleshed sweet potato (PFSP) cultivars are available for Brazilian growers, urging for the development of new and adapted cultivars. PFSP breeding programs should include multiple traits during the selection process to increase the chances of developing an adequate genotype, specially yield-related and quality-related traits. The objective was to select promising PFSP genotypes based on yield and tuberous-roots-related traits exploring genotype x environment interaction (GEI). Four experiments were carried out, in which 23 pre-selected PFSP genotypes were evaluated based on yield and quality traits, after screening among 2500 experimental genotypes. The first experiment adopted an augmented block design, with ‘Luiza’ interspersed as a control. From this first experiment, the 19 experimental genotypes with the best performance were selected for the following three experiments, which were conducted in a randomized block experimental design, with three replications each, including ‘Luiza’ as control. The best genotypes were selected in the first experiment using the multi-trait genotype-ideotype distance index (MGIDI). For the following experiments, the performance of the genotypes was assessed using deviance analysis, genotypic stability through weighted average of absolute scores (WAASB) index, and the multi-trait stability index (MTSI). In the first experiment, three factors were retained, being associated to commercial production of tuberous roots (‘K-104′, ‘K-25′ and ‘U1–29′), total production of tuberous roots (‘K-110′, ‘C-42′, ‘F-16′, and ‘U1–29′), and quality of tuberous roots (‘F-22′), respectively. Desired gains were observed for all traits. For the experiments II, III, and IV five factors were retained, being four related to quality of tuberous roots and one to yield components. The GEI was significant for yield-related traits and desired gains were observed for most traits. Due to high commercial tuberous root yield and low WAASB values, the genotypes ‘U1–29′, ‘K-98′, ‘F-22′, ‘K-57′, ‘C-36′, ‘U1–15′, ‘K-25′, and ‘U2–12′ were highly productive and stable. The genotypes selected by the MTSI index were ‘U1–15′, ‘U2–10′, ‘U1–29′, ‘K-98′, and ‘K-78′. The multi-trait selection enabled the identification of promising genotypes highlighting their strengths and weaknesses.
可供巴西种植者使用的紫肉甘薯(PFSP)栽培品种很少,因此需要开发新的、适应性强的栽培品种。紫茎甘薯育种计划应在选育过程中包括多种性状,以增加培育出适当基因型的机会,特别是与产量和质量相关的性状。目的是根据产量和块茎根相关性状,探索基因型与环境的交互作用(GEI),筛选出有潜力的 PFSP 基因型。在 2500 个实验基因型中筛选出 23 个预选的 PFSP 基因型后,根据产量和品质性状对其进行了评估。第一项实验采用了扩增区组设计,以 "Luiza "作为对照。从第一项实验中选出 19 个表现最好的实验基因型,用于随后的三项实验,这三项实验采用随机区组实验设计,每项实验有三个重复,其中包括作为对照的 "Luiza"。第一项实验采用多性状基因型-异位型距离指数(MGIDI)选出最佳基因型。在接下来的实验中,使用偏差分析、加权平均绝对得分(WAASB)指数和多性状稳定性指数(MTSI)评估基因型的表现。在第一个试验中,保留了三个因子,分别与块根的商品产量('K-104′、'K-25′和'U1-29′)、块根的总产量('K-110′、'C-42′、'F-16′和'U1-29′)以及块根的质量('F-22′)有关。所有性状都取得了预期的增产效果。在试验 II、III 和 IV 中,保留了五个因子,其中四个与块根质量有关,一个与产量成分有关。GEI 对与产量相关的性状有显著影响,大多数性状都有预期收益。由于商品块根产量高、WAASB 值低,基因型'U1-29′、'K-98′、'F-22′、'K-57′、'C-36′、'U1-15′、'K-25′和'U2-12′产量高且稳定。通过 MTSI 指数筛选出的基因型有'U1-15′、'U2-10′、'U1-29′、'K-98′和'K-78′。通过多性状筛选,确定了有潜力的基因型,突出了它们的优缺点。
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引用次数: 0
Assessing genotypic diversity by multivariate analysis and predicting useful selection ranges using decision tree in soybean (Glycine max L.) 通过多变量分析评估大豆(Glycine max L.)的基因型多样性并利用决策树预测有用的选择范围
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-25 DOI: 10.1016/j.sajb.2024.09.045
Volkan Mehmet Çınar
The existence of sufficient genetic variability and knowing the suitable selection criterion ranges are indispensable requirements of breeding studies. This study aimed to explore genetic diversity in ten F2 populations (heterozygous–heterogeneous), five parents, and three commercial check cultivars by multivariate methods and to predict useful selection ranges using a decision tree model for further breeding studies. Hybridizations were made in 2019, F1 populations were grown, and F2 seeds were obtained in 2020. Genotypes were grown according to an Augmented Randomized Complete-Block Design in 2021 at Aydın Adnan Menderes University Faculty of Agriculture. Results revealed considerable genetic diversity and transgressive segregation in key characteristics across the F2 populations. Seed yields of F2 plants are higher than those of the parents. The seed protein and oil content of the F2 plants were not different from the parents and commercial checks. Higher phenotypic than genotypic variance indicated a high influence of environment on the inheritance of all studied characteristics. The highest broad heritability was observed in the number of shattered pods per plant (94.81 %) and the lowest in infertile pods per plant (2.16 %). Seed yield showed a significant and positive genotypic correlation with the number of fertile pods per plant (0.61**) and a significant negative association with the number of fertile branches per plant (−0.53**). Principal component analysis (PCA) of 14 quantitative characteristics showed that soybean genotypes separated by the first four components contributed 71.12 % of total genetic variation, with important yield-attributing characteristics present in PC1 and PC3, while important seed quality characteristics were noted in PC2. The decision tree model indicated that genotypes with high yield and optimum quality could be obtained by selecting the number of fertile pods 87–119 per plant and a hundred-seed weight greater than 14.55 g.
存在足够的遗传变异和了解合适的选择标准范围是育种研究不可或缺的要求。本研究旨在通过多元方法探索 10 个 F2 群体(杂合子-异质性)、5 个亲本和 3 个商业对照栽培品种的遗传多样性,并利用决策树模型预测有用的选择范围,以便进一步开展育种研究。2019 年进行杂交,培育 F1 群体,2020 年获得 F2 种子。2021 年,在艾登阿德南-门德尔内斯大学农学院根据 "增强随机完全区组设计"(Augmented Randomized Complete-Block Design)培育基因型。结果显示,F2 群体的主要特征具有相当大的遗传多样性和转基因分离。F2 植株的种子产量高于亲本。F2 植株的种子蛋白质和油含量与亲本和商业对照没有差异。表型方差高于基因型方差表明环境对所有研究特性的遗传有很大影响。每株碎荚数的广泛遗传率最高(94.81%),每株不孕荚数的广泛遗传率最低(2.16%)。种子产量与每株可育豆荚数呈显著正相关(0.61**),与每株可育分枝数呈显著负相关(-0.53**)。14 个数量特征的主成分分析(PCA)显示,由前四个成分分隔的大豆基因型占总遗传变异的 71.12%,重要的产量特征出现在 PC1 和 PC3 中,而重要的种子质量特征则出现在 PC2 中。决策树模型表明,通过选择每株可育豆荚数为 87-119 个且百粒种子重量大于 14.55 克的基因型,可获得高产和最佳质量的基因型。
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引用次数: 0
Bambara groundnut (Vigna subterranean L.): An underutilized source of plant protein 班巴拉花生(Vigna subterranean L.):一种未得到充分利用的植物蛋白来源
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.sajb.2024.09.007
Harpreet Kaur Saini , Anshu Sharma , Devina Vaidya , Manisha Kaushal , Anupama Anand , Ritesh Verma , Akshita Sharma
Bambara groundnut (BG) is a particularly valuable underutilized crop indigenous to Africa that has gained popularity due to its high adaptation to climate change, drought tolerance and pest and disease resistance. It is chiefly cultivated as a plant-based protein alternative (containing 18–30 % protein) to meat in rural cuisines rather than an oil-producing seed, as commonly seen with other legumes. BG contains all the essential and non-essential amino acids in sufficient quantities to fulfil dietary and functional requirements in diverse food systems. This review delves into the techniques employed for the extraction/ isolation of BG proteins and their characterization. Different methods for the hydroxylation of BG proteins have been comprehensively reviewed and the relation between biological activity of BG peptides and amino acid composition has also been established. Comprehensive insights are given into the diverse techno-functional attributes of legume proteins, covering aspects like solubility, water and oil absorption capacity, emulsification, foaming, and gelling ability. The text also emphasizes the practical applications of these proteins across a range of products, viz., bakery items, extruded snacks, therapeutic foods and desserts. New developments in BG protein research have been outlined along with recommendations for future research and applications, encouraging its widespread utilization across the food sector and facilitating a successful transition toward more sustainable plant-based diets.
班巴拉落花生(Bambara groundnut,BG)是非洲土生土长的一种特别珍贵的未充分利用的作物,由于其对气候变化的高度适应性、耐旱性和抗病虫害性而广受欢迎。在农村菜肴中,它主要作为一种植物蛋白替代肉类(含 18-30% 蛋白质)而种植,而不是像其他豆科植物那样作为产油种子。麸皮含有所有必需氨基酸和非必需氨基酸,数量充足,可满足不同食物系统的饮食和功能需求。本综述深入探讨了提取/分离 BG 蛋白质及其表征所采用的技术。全面综述了 BG 蛋白羟基化的不同方法,并确定了 BG 肽的生物活性与氨基酸组成之间的关系。文中全面介绍了豆类蛋白质的各种技术功能属性,包括溶解性、吸水和吸油能力、乳化、发泡和胶凝能力等方面。文中还强调了这些蛋白质在烘焙食品、挤压点心、食疗食品和甜点等一系列产品中的实际应用。书中概述了 BG 蛋白质研究的新进展,并对未来的研究和应用提出了建议,鼓励在食品领域广泛使用 BG 蛋白质,促进向更可持续的植物性饮食成功过渡。
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引用次数: 0
Studies on pharmacological aspects, integrated pest management and economic importance of Rosa damascena L 关于大马士革蔷薇(Rosa damascena L)药理、虫害综合防治和经济重要性的研究
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.sajb.2024.08.032
Mohammad Afaan Fazili , Irfan Bashir Ganie , Qazi Parvaiz Hassan
This review highlights recent advancements in the utilization of rose oil across medicinal, pharmaceutical, therapeutic, and clinical trials, as well as its integration into pest management strategies that significantly contribute to its economic value. The Damask Rose, recognized for its potent pharmacological properties, serves multiple roles including as an anti-HIV agent, antioxidant source, antitussive, hypnotic, anti-diabetic treatment, and tracheal system soother. There is a crucial focus on enhancing the content of aromatic monoterpenes and sesquiterpenes in Rosa damascena L. Clinical studies affirm that rose oil effectively addresses various physiological and psychological conditions such as pain relief, anxiety reduction, depression alleviation, respiratory system improvement, blood oxygenation, pulse regulation, skin temperature control, stress management, menstrual discomfort, and exhibits antifungal, antibacterial properties, and potential as a sex stimulant. Rose cultivation faces challenges from numerous pests including diseases, insects, nematodes, and weeds, which detrimentally affect yield and flower quality. Effective management of these pests ensures optimal returns for rose growers.
本综述重点介绍了玫瑰精油在药用、制药、治疗和临床试验方面的最新进展,以及将其纳入害虫管理策略的情况,这些都大大提高了玫瑰精油的经济价值。大马士革玫瑰被公认为具有强大的药理特性,可发挥多种作用,包括抗艾滋病毒、抗氧化、止咳、催眠、抗糖尿病治疗和舒缓气管系统。临床研究证实,玫瑰精油能有效解决各种生理和心理问题,如止痛、减轻焦虑、缓解抑郁、改善呼吸系统、血液供氧、脉搏调节、皮肤温度控制、压力管理、月经不适,并具有抗真菌、抗菌特性和作为性兴奋剂的潜力。玫瑰种植面临着许多虫害的挑战,包括疾病、昆虫、线虫和杂草,这些都会对产量和花朵质量产生不利影响。有效防治这些害虫可确保玫瑰种植者获得最佳收益。
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引用次数: 0
Decoding eggplant fruit: Multi-omics profiling of caffeoyl-CoA-3-OMT expression 解码茄子果实:咖啡酰-CoA-3-OMT表达的多组学分析
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.sajb.2024.09.044
Kirtan Dave , Prashant Kaushik , Nil Patil , Rupal Dhariwal , Meenakshi Sharma , Alpa Yadav , Parmdeep Singh Dhanda , Mukul Jain
Eggplant (Solanum melongena L.) is a rich source of health-promoting phenolic acids, mainly chlorogenic acid (CGA), and trace elements. CGA has been shown to have antioxidant, anti-inflammatory, neuroprotective, cardioprotective, anticancer, and antidiabetic properties. The natural genome of eggplants contains essential genes for beneficial traits like drought tolerance, disease resistance, and high concentrations of medicinal substances. Transcriptomic analysis of eggplant overexpressing the hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase (HQT) gene revealed upregulation of 2165 genes, including three similar to HQT and cinnamate-4-hydroxylase (C4H). Sequence similarity analysis showed homology to caffeoyl-CoA O-methyltransferases, key enzymes in the phenylpropanoid pathway leading to CGA biosynthesis. Gene ontology and KEGG pathway analysis identified 5 highly upregulated glycosyltransferase family 43 genes (650.3-fold change). Glycosyltransferases like UDP-glucose:cinnamate glucosyltransferase and cinnamate-glucose 4′-O-glucosyltransferase are crucial for CGA production in eggplant. qRT-PCR validated the potential upregulation of HQT and C4H in transgenic eggplants. Comparative analysis revealed eggplant has the highest CGA content (5–8.1 g/kg dry weight) among vegetables, with foliar CGA acting as a natural insecticide. CGA offers antioxidant, hypoglycemic, antiviral, and hepatoprotective benefits to humans. This study highlights the role of HQT and C4H in elevating the medicinal properties of eggplant through CGA biosynthesis pathway engineering.
茄子(Solanum melongena L.)是促进健康的酚酸(主要是绿原酸(CGA))和微量元素的丰富来源。研究表明,绿原酸具有抗氧化、抗炎、保护神经、保护心脏、抗癌和抗糖尿病的功效。茄子的天然基因组中含有耐旱、抗病和高浓度药用物质等有益性状的重要基因。对过表达羟基肉桂酰辅酶醌羟基肉桂酰转移酶(HQT)基因的茄子进行转录组分析,发现有2165个基因上调,其中包括三个与HQT和肉桂酸-4-羟化酶(C4H)相似的基因。序列相似性分析表明,该基因与咖啡酰-CoA O-甲基转移酶同源,而咖啡酰-CoA O-甲基转移酶是导致 CGA 生物合成的苯丙酮途径中的关键酶。基因本体和 KEGG 通路分析确定了 5 个高度上调的糖基转移酶家族 43 基因(变化了 650.3 倍)。糖基转移酶如 UDP-葡萄糖:肉桂酸葡萄糖基转移酶和肉桂酸-葡萄糖 4′-O-葡萄糖基转移酶对茄子 CGA 的产生至关重要。qRT-PCR 验证了转基因茄子中 HQT 和 C4H 的潜在上调。比较分析表明,在蔬菜中,茄子的 CGA 含量最高(5-8.1 克/千克干重),叶面 CGA 可作为天然杀虫剂。CGA 具有抗氧化、降血糖、抗病毒和保护肝脏等功效。本研究强调了 HQT 和 C4H 在通过 CGA 生物合成途径工程提升茄子药用价值方面的作用。
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引用次数: 0
Phytochemical profiling, therapeutic target enzymes inhibitory, antioxidant, and cytotoxic potential of Cardopatium corymbosum 红豆杉的植物化学成分分析、治疗靶酶抑制、抗氧化和细胞毒性潜力
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.sajb.2024.09.025
Gozde Hasbal-Celikok , Nermin Çakmak , Yasin Celikok , Servet Duranay , Bahar Gürdal , Ebru Özdemir Nath , Tugba Yilmaz-Ozden
Cardopatium corymbosum (L.) Pers. (Asteraceae) has been used ethnomedicinally against intestinal worms, for skin diseases, and in wound treatment. This study investigated the phytochemical profile and biological activities of ethanol (CCE), methanol (CCM), and water (CCW) extracts of C. corymbosum. LC-MS/MS analysis revealed the presence of various metabolites. Antioxidant activities of the extracts were determined by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities and ferric-reducing power (FRAP). The cytotoxicity against human breast cancer (MCF-7) and human umbilical vein endothelial (HUVEC) cell lines were determined using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Enzyme inhibitory activities against α-amylase, α-glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), thioredoxin reductase (TrxR), and elastase were measured to assess potential antidiabetic, anti-Alzheimer's, anticancer, and antiaging/wound healing effects. CCM exhibited the highest total phenolic and flavonoid contents and antioxidant activity. Fumaric acid, chlorogenic acid, vanillic acid, and rutin were identified as major components in CCM. C. corymbosum extracts, particularly those from CCE and CCM, displayed significant cytotoxicity on MCF-7 cells while exhibiting lower toxicity towards HUVEC cells. Notably, CCM demonstrated potent α-glucosidase inhibitory activity, while no α-amylase inhibition was observed. All extracts showed weak inhibition of AChE, BChE, and elastase. Conversely, the extracts displayed moderate TrxR inhibitory activity. These findings suggest that C. corymbosum possesses significant antioxidant, enzyme inhibitory, and cytotoxic potential, highlighting its promise as a source of novel bioactive molecules.
Cardopatium corymbosum (L.) Pers.(菊科)在民族医药中被用于防治肠道蠕虫、治疗皮肤病和伤口。本研究调查了 C. corymbosum 的乙醇(CCE)、甲醇(CCM)和水(CCW)提取物的植物化学成分和生物活性。LC-MS/MS 分析揭示了各种代谢物的存在。通过测定 2,2-二苯基-1-苦基肼(DPPH)自由基清除活性和铁还原力(FRAP),确定了提取物的抗氧化活性。使用(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑)(MTT)试验测定了对人乳腺癌(MCF-7)和人脐静脉内皮细胞(HUVEC)的细胞毒性。测定了α-淀粉酶、α-葡萄糖苷酶、乙酰胆碱酯酶(AChE)、丁酰胆碱酯酶(BChE)、硫氧还原酶(TrxR)和弹性蛋白酶的酶抑制活性,以评估潜在的抗糖尿病、抗老年痴呆、抗癌和抗衰老/伤口愈合作用。中药的总酚和类黄酮含量以及抗氧化活性最高。经鉴定,富马酸、绿原酸、香草酸和芦丁是中药的主要成分。C. corymbosum 提取物,尤其是 CCE 和 CCM 提取物,对 MCF-7 细胞具有显著的细胞毒性,而对 HUVEC 细胞的毒性较低。值得注意的是,CCM 对α-葡萄糖苷酶有很强的抑制活性,而对α-淀粉酶没有抑制作用。所有提取物对 AChE、BChE 和弹性蛋白酶都有微弱的抑制作用。相反,提取物显示出中等的 TrxR 抑制活性。这些研究结果表明,C. corymbosum 具有显著的抗氧化、酶抑制和细胞毒性潜力,有望成为新型生物活性分子的来源。
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引用次数: 0
Auxinic pulse induces direct somatic embryogenesis in Plinia peruviana (Poir.) Govaerts (Myrtaceae) 叶绿素脉冲诱导 Plinia peruviana (Poir.) Govaerts(桃金娘科)直接体细胞胚胎发生
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.sajb.2024.09.040
Daniele Damian dos Santos , Márcia Regina Faita , Luana Oliveira de Oliveira , Dalvan Carlos Beise , Rosete Pescador , Miguel Pedro Guerra , Valdir Marcos Stefenon
Plinia peruviana (Myrtaceae) is a fruit tree species endemic to Brazil with high importance to the food and pharmacological industries. Despite the need for vegetative propagation of selected genotypes for genetic breeding or fruit production, efficient propagation methods for the species have not yet been determined. This study aimed to advance the establishment of a protocol for inducing somatic embryogenesis from mature P. peruviana seeds. Three concentrations of 2,4-dichlorophenoxyacetic acid (10, 20, and 30 µM) in four auxinic pulses (1, 3, 5, and 7 days of exposure times) were tested. 20 µM and 30 µM of 2,4-D promoted the highest callogenesis (20–40 %), regardless of the exposure period, but calli did not present embryogenic potential. The formation of somatic embryos occurred asynchronously directly on the embryonic axes and was dependent on adding 2,4-D to the culture medium. The highest induction rate of direct somatic embryogenesis (26.7 %) was obtained using 20 µM of 2,4-D for 5 days of exposure. The light and electronic microscopic analyses allowed the characterization of the embryos at different stages of development and identified some abnormalities. Additional improvements to the protocol are still needed, including testing different culture media since no conversion and formation of complete plantlets was achieved.
Plinia peruviana(桃金娘科)是巴西特有的果树品种,在食品和制药业中具有重要地位。尽管需要对选定的基因型进行无性繁殖以进行遗传育种或果实生产,但该物种的高效繁殖方法尚未确定。本研究旨在推动建立一套方案,从成熟的 P. peruviana 种子中诱导体细胞胚胎发生。该研究测试了四种助长脉冲(1、3、5 和 7 天的暴露时间)中三种浓度的 2,4-二氯苯氧乙酸(10、20 和 30 µM)。无论暴露时间长短,20 µM 和 30 µM 的 2,4-D 都能促进最高的胼胝发生率(20-40%),但胼胝体没有胚胎发生潜能。体细胞胚的形成直接在胚轴上异步发生,取决于在培养基中添加 2,4-D。使用 20 µM 的 2,4-D 进行 5 天的暴露,体细胞胚胎直接形成的诱导率最高(26.7%)。通过光镜和电子显微镜分析,可以确定胚胎在不同发育阶段的特征,并发现一些异常情况。由于没有实现转化和形成完整的小植株,因此仍需对方案进行进一步改进,包括测试不同的培养基。
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引用次数: 0
The insights into the activity of the extracts from Polygonum aviculare L. and Pseudomonas fluorescens for enhancing and modeling seed germination and seedling growth of Melilotus officinalis L. Lam 从何首乌和荧光假单胞菌中提取的提取物在增强和模拟 Melilotus officinalis L. Lam 种子萌发和幼苗生长方面的活性的深入研究
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.sajb.2024.09.028
Agnieszka Szparaga , Ewa Czerwińska , Ireneusz Kapusta , Joanna Piepiórka-Stepuk , Grzegorz Zaguła , Łukasz Szparaga , Gianluca Caruso , Beata Erlichowska , Ewa Deszcz
The empirical evidence indicated that chemicals in plants and selected microorganisms can promote the germination process and determine the growth, viability, survival and yield potential of industrial crop plants. The main objective of this study was exploring the phytochemical and microbiological synergistic activity between plant extracts from Polygonum aviculare L. and beneficial bacteria Pseudomonas fluorescens to enhance germination of Melilotus officinalis seeds and improve morphological characteristics of seedlings. The derived plant preparations were analyzed qualitatively, which showed that the aqueous extracts of P. aviculare were characterized by a higher content of phenolic compounds, micro- and macroelements and carbohydrates compared to the macerates, and that no phytohormones were detected in both the infusions and macerates. Pure macerates (MAC), infusions (IF), bacterial cultures (PF) and their mixtures (MAC+PF; INF+PF) were used for seed treatment of test plants. In the control group, lower values of M. officinalis seed germination and seedling growth rates were observed in relation to the applied treatments. The most effective germination rates were obtained after the application of pure infusions (IF) and their mixture with P. fluorescens (IF+PF), while the highest growth rates (including an almost twofold increase in the length of the nostril shoots compared to the control) were obtained after the application of P. fluorescens inoculum (PF) and the mixture of the infusion and these bacteria (IF+PF).
The use of modeling, as a tool for predicting germination efficiency, has been shown to provide screening opportunities for identifying the effectiveness of biostimulant treatments. Thereby, this indicates the need to develop such an approach under field conditions.
经验证据表明,植物中的化学物质和选定的微生物可促进发芽过程,并决定工业作物植物的生长、活力、存活率和产量潜力。本研究的主要目的是探索何首乌植物提取物与有益细菌荧光假单胞菌之间的植物化学和微生物协同活性,以提高 Melilotus officinalis 种子的萌发率并改善幼苗的形态特征。对提取的植物制剂进行了定性分析,结果表明,与浸渍剂相比,何首乌水提取物的酚类化合物、微量元素、大分子元素和碳水化合物含量更高,而且在浸泡剂和浸渍剂中都没有检测到植物激素。纯浸渍剂(MAC)、输液(IF)、细菌培养物(PF)及其混合物(MAC+PF;INF+PF)被用于试验植物的种子处理。在对照组中,观察到的 M. officinalis 种子发芽率和幼苗生长率均低于所使用的处理方法。使用纯输液(IF)及其与 P. fluorescens 的混合物(IF+PF)后,发芽率最高,而使用 P. fluorescens 接种物(IF+PF)后,生长率最高(包括鼻芽长度比对照组几乎增加了两倍)。建模作为预测发芽效率的一种工具,为确定生物刺激剂处理的有效性提供了筛选机会。因此,这表明有必要在实地条件下开发这种方法。
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引用次数: 0
Nutritional, antinutritional and antioxidant assessment of immature Abelmoschus pods: Implications for crop improvement from cultivated and wild varieties 未成熟阿贝尔莫斯卡豆荚的营养、抗营养和抗氧化评估:利用栽培品种和野生品种改良作物的意义
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-23 DOI: 10.1016/j.sajb.2024.09.022
Amandeep Kaur , Manjeet Kaur Sangha , Veena Devi , Mamta Pathak , Diksha Singla
Crop wild relatives hold significant potential for enhancing diversity, nutrition, and stress resilience in improvement programs. As such thorough, the biochemical assessment of these wild counterparts becomes indispensable. Okra, a vital vegetable crop renowned for its vibrant green pods, faces various productivity challenges due to diverse stresses, and its nutritional and stress tolerance aspects have been somewhat overlooked. In this current research, an extensive evaluation was conducted on both wild and cultivated okra species to decipher their nutritional, anti-nutritional, and antioxidant profiles. The results of the proximate analysis showed that wild okra exhibited higher nutritional value and antioxidant potential compared to cultivated species. Remarkably among the wild species A. moschatus accessions IC 470737 and IC 140986 stood out for their elevated content of carbohydrates (93.10 % and 69.38 %), soluble sugars (84.36 % and 41.83 %), reducing sugars (19.41 % and 38.11 %), and vitamin E (37.23 % and 51.24 %); whereas A. Manihot and A. tetraphyllus had higher antioxidant potential. Significantly, these wild entries also showcased the reduced anti-nutrient content suggesting high mineral bioavailability compared to cultivated ones. So, these wild genotypes having a good combination of many biochemical characteristics can be used for the introgression of traits in cultivated okra for further end product diversification.
作物野生近缘种在改良计划中具有提高多样性、营养和抗逆性的巨大潜力。因此,对这些野生近缘植物进行生化评估是必不可少的。秋葵是一种重要的蔬菜作物,以其充满活力的绿色豆荚而闻名,但由于受到各种压力的影响,它的产量面临着各种挑战,其营养和抗逆性方面的问题在某种程度上被忽视了。本次研究对野生和栽培秋葵品种进行了广泛评估,以解读它们的营养、抗营养和抗氧化特性。近似分析结果表明,野生秋葵的营养价值和抗氧化潜力均高于栽培品种。值得注意的是,野生秋葵品种IC 470737和IC 140986的碳水化合物(93.10%和69.38%)、可溶性糖(84.36%和41.83%)、还原糖(19.41%和38.11%)和维生素E(37.23%和51.24%)含量较高;而A. Manihot和A. tetraphyllus的抗氧化潜力较高。值得注意的是,与栽培品种相比,这些野生品种的抗营养素含量也有所降低,表明矿物质的生物利用率较高。因此,这些野生基因型具有多种生化特性的良好组合,可用于栽培秋葵的性状导入,以进一步实现最终产品的多样化。
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引用次数: 0
Improving antioxidant and anti-hyperglycemic potential of germinating fenugreek seeds through natural phenolic elicitors 通过天然酚类激发剂提高发芽葫芦巴种子的抗氧化和降血糖潜力
IF 2.7 3区 生物学 Q2 PLANT SCIENCES Pub Date : 2024-09-23 DOI: 10.1016/j.sajb.2024.09.029
Omi Laıla , Imtıyaz Murtaza , Mir Rashid , Sofi Imtiyaz Ali , Sheikh Abid Ali , Tariq A Raja
The present study investigated the use of natural elicitors (vitamin C, folic acid, and lactoferrin) to stimulate the phenylpropanoid pathway in germinating fenugreek sprouts, with the aim of increasing their total phenolic phytochemical compounds responsible for imparting antioxidant and anti-hyperglycemic properties. Observations revealed that germinating fenugreek sprouts of the IM3 genotype, pre-treated with 500 µM vitamin C (T1) on the 4th day, exhibited maximal elicitation of total phenolic content (3680 mg/100 g DW) and antioxidant activity (2607.5 µM/100 g DW) compared to other genotypes. Moreover, T1-treated IM3 fenugreek sprouts demonstrated the highest anti-hyperglycemic activity by inhibiting α-amylase (48.96%), α-glucosidase (92.60%), and invertase (45.65%) enzyme activities under in vitro conditions. Interestingly, the selected treatments did not affect the diosgenin and trigonelline content of germinating sprouts, which decreased in a time-dependent manner during germination. However, the quercetin content (0.01365%) of T1-treated germinating sprouts continued to increase appreciably, even after the 4th day of germination. A direct positive correlation was established between the increase in total phenols, especially quercetin, and the antioxidant potential as well as the anti-hyperglycemic activity in germinated fenugreek sprouts on the 4th day. Thus, T1-treated sprouts hold promise for managing diabetes-related hyperglycemia and oxidative stress, emphasizing the efficacy of vitamin C in enhancing their bioactive properties. This research pioneers the use of natural elicitors to enhance the health benefits of fenugreek sprouts, notably by identifying an optimal treatment and elucidating quercetin dynamics during germination. Such findings are pivotal for advancing our understanding of sprouting processes and provide valuable insights into the development of therapeutic functional foods. However, further research is necessary to validate their efficacy before considering them as futuristic functional foods.
本研究调查了使用天然诱导剂(维生素 C、叶酸和乳铁蛋白)来刺激发芽的胡芦巴芽中的苯丙类途径,目的是增加其总酚植物化学物质,从而赋予其抗氧化和抗高血糖特性。观察结果表明,在第 4 天用 500 µM 维生素 C(T1)预处理的 IM3 基因型葫芦巴发芽芽与其他基因型相比,总酚含量(3680 mg/100 g DW)和抗氧化活性(2607.5 µM/100 g DW)均达到最高水平。此外,在体外条件下,T1 处理的 IM3 葫芦巴芽通过抑制 α-淀粉酶(48.96%)、α-葡萄糖苷酶(92.60%)和转化酶(45.65%)的酶活性,表现出最高的抗高血糖活性。有趣的是,所选的处理方法并不影响发芽芽中的薯蓣皂苷和三尖杉酯碱含量,它们在发芽过程中的下降与时间有关。然而,经 T1 处理的发芽芽中的槲皮素含量(0.01365%)持续显著增加,甚至在发芽第 4 天后也是如此。总酚(尤其是槲皮素)的增加与第 4 天发芽的葫芦巴菜芽的抗氧化潜力和抗高血糖活性之间存在直接的正相关关系。因此,经 T1 处理的芽菜有望控制与糖尿病相关的高血糖和氧化应激,并强调了维生素 C 在增强其生物活性特性方面的功效。这项研究开创性地利用天然诱导剂来提高葫芦巴芽的保健功效,特别是通过确定最佳处理方法和阐明槲皮素在发芽过程中的动态变化。这些发现对于加深我们对发芽过程的了解至关重要,并为开发治疗性功能食品提供了宝贵的见解。不过,在将它们视为未来的功能食品之前,有必要开展进一步的研究来验证它们的功效。
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引用次数: 0
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South African Journal of Botany
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