Pub Date : 2018-08-30DOI: 10.1142/S1682648518500026
Kuo-Hua Lee, Pi-Chu Nien, T. Chen, C. Yeh, Jih-Yih Chen, Wen-Hung Lin, Tzu-Tai Lee, Y. Jea, C. Chi
The study was conducted to investigate the effects of Ganoderma lucidum (GL) on growth performance, survival rates, and immunocompetence of the weaning pigs. A total of 45, 28-day-old Landrace weaning pigs were randomly divided into three groups of 15 pigs each. A corn–soybean meal-based control diet was formulated and the experimental diets included 0.3% and 0.6% GL powder. The groups were treated for 38 days. Pigs which fed both concentration of GL powder had significantly higher ([Formula: see text]) survival rates than the control group. Besides, pigs which fed 0.3% and 0.6% GL powder had increased lymphocyte proliferation indexes ([Formula: see text]) and the titer of neutralizing antibody against hog cholera (HC) ([Formula: see text]) on day 66 compared with pigs which fed control diet. No differences were found between treatments regarding the average daily gain (ADG), gain:feed (G:F) ratio and diarrhea score. Supplementation of GL powder showed no effects on aspartate aminotransferase (AST) and blood urea nitrogen (BUN), which suggested that GL treatment has no immediate harm on liver or kidney. Based on these results, GL could be suggested as a potential feed additive of weaning pig diets to increase survival rate and immunocompetence.
{"title":"DIETARY SUPPLEMENTATION OF GANODERMA LUCIDUM POWDER ENHANCES SURVIVAL AND IMMUNOCOMPETENCE OF WEANING PIGS","authors":"Kuo-Hua Lee, Pi-Chu Nien, T. Chen, C. Yeh, Jih-Yih Chen, Wen-Hung Lin, Tzu-Tai Lee, Y. Jea, C. Chi","doi":"10.1142/S1682648518500026","DOIUrl":"https://doi.org/10.1142/S1682648518500026","url":null,"abstract":"The study was conducted to investigate the effects of Ganoderma lucidum (GL) on growth performance, survival rates, and immunocompetence of the weaning pigs. A total of 45, 28-day-old Landrace weaning pigs were randomly divided into three groups of 15 pigs each. A corn–soybean meal-based control diet was formulated and the experimental diets included 0.3% and 0.6% GL powder. The groups were treated for 38 days. Pigs which fed both concentration of GL powder had significantly higher ([Formula: see text]) survival rates than the control group. Besides, pigs which fed 0.3% and 0.6% GL powder had increased lymphocyte proliferation indexes ([Formula: see text]) and the titer of neutralizing antibody against hog cholera (HC) ([Formula: see text]) on day 66 compared with pigs which fed control diet. No differences were found between treatments regarding the average daily gain (ADG), gain:feed (G:F) ratio and diarrhea score. Supplementation of GL powder showed no effects on aspartate aminotransferase (AST) and blood urea nitrogen (BUN), which suggested that GL treatment has no immediate harm on liver or kidney. Based on these results, GL could be suggested as a potential feed additive of weaning pig diets to increase survival rate and immunocompetence.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74865067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-10DOI: 10.1142/S1682648518720022
I. Chan, Tung Hsueh, Wen-Ying Chen
Two 8-year-old small-breed neutered dogs were referred due to respiratory distress caused by a new onset of congestive heart failure secondary to degenerative mitral valve disease. Medical treatment, including a diuretic and a vasodilator, was initiated, and blood samples for evaluating the levels of blood urea nitrogen, creatinine, and N-terminal pro-brain natriuretic peptide (NT-proBNP) were collected at every visit. The two dogs had entirely different outcomes: one is still alive with its symptoms well controlled by the medications, whereas the other one was unstable during the therapeutic period and only survived for 89 days after the diagnosis was made. Serial measurements of NT-proBNP concentration revealed that its gradual decline corresponded to better outcome, whereas fluctuant and ascending concentrations reflected a poor prognosis.
{"title":"CHANGES IN SERUM CONCENTRATION OF N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE AFTER TREATMENT IN TWO DOGS WITH CONGESTIVE HEART FAILURE SECONDARY TO MITRAL VALVE DISEASE","authors":"I. Chan, Tung Hsueh, Wen-Ying Chen","doi":"10.1142/S1682648518720022","DOIUrl":"https://doi.org/10.1142/S1682648518720022","url":null,"abstract":"Two 8-year-old small-breed neutered dogs were referred due to respiratory distress caused by a new onset of congestive heart failure secondary to degenerative mitral valve disease. Medical treatment, including a diuretic and a vasodilator, was initiated, and blood samples for evaluating the levels of blood urea nitrogen, creatinine, and N-terminal pro-brain natriuretic peptide (NT-proBNP) were collected at every visit. The two dogs had entirely different outcomes: one is still alive with its symptoms well controlled by the medications, whereas the other one was unstable during the therapeutic period and only survived for 89 days after the diagnosis was made. Serial measurements of NT-proBNP concentration revealed that its gradual decline corresponded to better outcome, whereas fluctuant and ascending concentrations reflected a poor prognosis.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"123 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78588806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.1142/s1682648520500067
A. Liao, Wei-Hsiang Huang, Shang-Lin Wang
Canine pyometra is a common reproductive disease in bitches. This study evaluated the causative bacteria in antibiotic sensitivity test. Fifty five bitches with pyometra were included in this study. Escherichia coli was the most isolated microorganism (63.6%), followed by Streptococcus spp. (10.9%). Sixty two pathogen isolates showed highest sensitivity to imipenem (95.2%), followed by gentamicin (85.5%), amikacin (80.6%), ceftazidime (72.6%), nitrofurantoin (72.6%), and norfloxacin (72.6%). The microorganisms were most resistant to lincomycin (96.8%), followed by penicillin (85.5%), trimethoprim/sulfamethoxazole (58.1%), amoxicillin (54.8%), ampicillin (53.2%), and cefadroxil (50%). The sensitivity of a single empirical antibiotic treatment applied without sensitivity test was 51% (25/49). Sensitivity increased to 84.6% (11/13) when two antibiotics are used simultaneously. No significant differences in the proportions of clinical signs and abnormal hematologic results were observed between bitches with open- and closed-type pyometra. All bitches with pyometra recovered after ovariohysterectomy, antibiotic administration, and supportive therapy. The results of this study provide reliable information to choose an effective antibiotic before surgical intervention, especially for critical patients who cannot wait for the sensitivity test. The outcomes of the bitches with pyometra were excellent with early diagnosis and appropriate treatment.
{"title":"BACTERIAL ISOLATION AND ANTIBIOTIC SELECTION AFTER OVARIOHYSTERECTOMY OF CANINE PYOMETRA: A RETROSPECTIVE STUDY OF 55 CASES","authors":"A. Liao, Wei-Hsiang Huang, Shang-Lin Wang","doi":"10.1142/s1682648520500067","DOIUrl":"https://doi.org/10.1142/s1682648520500067","url":null,"abstract":"Canine pyometra is a common reproductive disease in bitches. This study evaluated the causative bacteria in antibiotic sensitivity test. Fifty five bitches with pyometra were included in this study. Escherichia coli was the most isolated microorganism (63.6%), followed by Streptococcus spp. (10.9%). Sixty two pathogen isolates showed highest sensitivity to imipenem (95.2%), followed by gentamicin (85.5%), amikacin (80.6%), ceftazidime (72.6%), nitrofurantoin (72.6%), and norfloxacin (72.6%). The microorganisms were most resistant to lincomycin (96.8%), followed by penicillin (85.5%), trimethoprim/sulfamethoxazole (58.1%), amoxicillin (54.8%), ampicillin (53.2%), and cefadroxil (50%). The sensitivity of a single empirical antibiotic treatment applied without sensitivity test was 51% (25/49). Sensitivity increased to 84.6% (11/13) when two antibiotics are used simultaneously. No significant differences in the proportions of clinical signs and abnormal hematologic results were observed between bitches with open- and closed-type pyometra. All bitches with pyometra recovered after ovariohysterectomy, antibiotic administration, and supportive therapy. The results of this study provide reliable information to choose an effective antibiotic before surgical intervention, especially for critical patients who cannot wait for the sensitivity test. The outcomes of the bitches with pyometra were excellent with early diagnosis and appropriate treatment.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76776762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.1142/s1682648518600016
{"title":"Proceedings of The Joint Spring Conference of the Chinese Society of Veterinary Science and the Taiwan Association of Veterinary Science and Animal Husbandry (CSVS/TAVSAH)","authors":"","doi":"10.1142/s1682648518600016","DOIUrl":"https://doi.org/10.1142/s1682648518600016","url":null,"abstract":"","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75914601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-02DOI: 10.1142/S1682648517500135
Ahmed H. Osman, M. Hala, A. Zakia, A. M. Mahmoud, M. Mohamed, A. Khattab, M. A. Z. Ewiss
This paper is considered as a part of a multi-disciplinary analysis “Application of Decentralized On-Site Water Treatment System in Egypt for Use in Agriculture and Producing Safe Animal Proteins”. The project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized Japanese’ Johkasou system. Blood chemistry of liver and kidney enzymes revealed significant increase in the fourth week in comparison with groups of treated and tap water. On the other hand, ALT and AST levels have no statistical differences between treated and tap water reared animals. The progressive pathological lesions were noted in 100% and 70% untreated water groups.The benefit of Johkasou system model CE10 in treatment of sewage water and suitability for rearing of animals is demonstrated.
{"title":"RABBIT’S HEALTH AND ENVIRONMENTAL IMPACTS OF EXPLOITATION TREATED BIODEGRADABLE POLLUTION WATER BY JOHKASOU SYSTEM","authors":"Ahmed H. Osman, M. Hala, A. Zakia, A. M. Mahmoud, M. Mohamed, A. Khattab, M. A. Z. Ewiss","doi":"10.1142/S1682648517500135","DOIUrl":"https://doi.org/10.1142/S1682648517500135","url":null,"abstract":"This paper is considered as a part of a multi-disciplinary analysis “Application of Decentralized On-Site Water Treatment System in Egypt for Use in Agriculture and Producing Safe Animal Proteins”. The project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized Japanese’ Johkasou system. Blood chemistry of liver and kidney enzymes revealed significant increase in the fourth week in comparison with groups of treated and tap water. On the other hand, ALT and AST levels have no statistical differences between treated and tap water reared animals. The progressive pathological lesions were noted in 100% and 70% untreated water groups.The benefit of Johkasou system model CE10 in treatment of sewage water and suitability for rearing of animals is demonstrated.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81142531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-07DOI: 10.1142/S1682648518500014
A. Stern, A. J. Young, J. Pieper
Ten adenocarcinomas of the apocrine gland of the anal sac and 11 hepatoid gland neoplasms were studied to determine the coordinate expression of cytokeratin 7 (CK7) and cytokeratin 14 (CK14) using commercially available antibodies. Hepatoid gland neoplasms included hepatoid gland adenomas, carcinomas and a single epithelioma. All 10 (100%) adenocarcinomas of the apocrine gland of the anal sac had CK7[Formula: see text]/CK14[Formula: see text] immunophenotype, whereas all 11 (100%) of hepatoid neoplasms expressed CK7[Formula: see text]/CK14[Formula: see text] immunophenotype. Hepatoid gland adenomas, carcinomas, and epithelioma could not be differentiated based on the cytokeratin immunophenotype. Hepatoid gland neoplasms could be differentiated from adenocarcinomas of the apocrine gland of the anal sac by differences in the CK7/CK14 immunophenotypes with a [Formula: see text]-[Formula: see text]. The results of this study provide further support for the use of coordinate expression of CK7/CK14 to differentiate apocrine gland adenocarcinomas of the anal sac (CK7[Formula: see text]/CK14[Formula: see text]) from hepatoid gland neoplasms (CK7[Formula: see text]/CK14[Formula: see text]).
{"title":"CYTOKERATIN PROFILES OF CANINE ANAL SAC AND HEPATOID GLAND NEOPLASMS","authors":"A. Stern, A. J. Young, J. Pieper","doi":"10.1142/S1682648518500014","DOIUrl":"https://doi.org/10.1142/S1682648518500014","url":null,"abstract":"Ten adenocarcinomas of the apocrine gland of the anal sac and 11 hepatoid gland neoplasms were studied to determine the coordinate expression of cytokeratin 7 (CK7) and cytokeratin 14 (CK14) using commercially available antibodies. Hepatoid gland neoplasms included hepatoid gland adenomas, carcinomas and a single epithelioma. All 10 (100%) adenocarcinomas of the apocrine gland of the anal sac had CK7[Formula: see text]/CK14[Formula: see text] immunophenotype, whereas all 11 (100%) of hepatoid neoplasms expressed CK7[Formula: see text]/CK14[Formula: see text] immunophenotype. Hepatoid gland adenomas, carcinomas, and epithelioma could not be differentiated based on the cytokeratin immunophenotype. Hepatoid gland neoplasms could be differentiated from adenocarcinomas of the apocrine gland of the anal sac by differences in the CK7/CK14 immunophenotypes with a [Formula: see text]-[Formula: see text]. The results of this study provide further support for the use of coordinate expression of CK7/CK14 to differentiate apocrine gland adenocarcinomas of the anal sac (CK7[Formula: see text]/CK14[Formula: see text]) from hepatoid gland neoplasms (CK7[Formula: see text]/CK14[Formula: see text]).","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90904006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-01DOI: 10.1142/S1682648517500111
Wei-Hsiang Huang, A. Liao, P. Chu, Shao-Hua Zhai, I-Feng Yen, Chen-Hsuan Liu
The advancement of animal welfare has attracted much public attention in recent years in Taiwan. Trauma, as an important extrinsic cause of death in pet dogs and cats, is closely related to human activities and owner–pet relationships. This retrospective study aims to evaluate trauma-related deaths in domesticated dogs and cats in Taiwan, and further investigate demographic risk factors associated with specific traumatic causes. Data from 2252 dogs and 1325 cats were collected from a real-time reporting system for mortality of Taiwanese domesticated dogs and cats during 2012–2014. A total of 278 trauma-related deaths, including 177 dogs and 101 cats, were reported. The leading traumatic causes were car accidents (dog/cat:127/48), falls (dog/cat:18/15), and animal interactions (dog/cat:13/11). Frequencies of trauma deaths were higher for animals within the 0–6 years age group in both species. Dogs and cats dying at <10 years of age, male and intact animals displayed increased risks of trauma deaths. Dogs, ...
{"title":"TRAUMA-RELATED DEATHS OF DOMESTICATED DOGS AND CATS IN TAIWAN","authors":"Wei-Hsiang Huang, A. Liao, P. Chu, Shao-Hua Zhai, I-Feng Yen, Chen-Hsuan Liu","doi":"10.1142/S1682648517500111","DOIUrl":"https://doi.org/10.1142/S1682648517500111","url":null,"abstract":"The advancement of animal welfare has attracted much public attention in recent years in Taiwan. Trauma, as an important extrinsic cause of death in pet dogs and cats, is closely related to human activities and owner–pet relationships. This retrospective study aims to evaluate trauma-related deaths in domesticated dogs and cats in Taiwan, and further investigate demographic risk factors associated with specific traumatic causes. Data from 2252 dogs and 1325 cats were collected from a real-time reporting system for mortality of Taiwanese domesticated dogs and cats during 2012–2014. A total of 278 trauma-related deaths, including 177 dogs and 101 cats, were reported. The leading traumatic causes were car accidents (dog/cat:127/48), falls (dog/cat:18/15), and animal interactions (dog/cat:13/11). Frequencies of trauma deaths were higher for animals within the 0–6 years age group in both species. Dogs and cats dying at <10 years of age, male and intact animals displayed increased risks of trauma deaths. Dogs, ...","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"199 1","pages":"15-26"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76033836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-01DOI: 10.1142/S168264851750010X
Chun-Hsiang Hu, T. Hsu, Kuan-Sheng Chen, Wei-Ming Lee, Hsien-Chi Wang
To evaluate the hemodynamic optimization effect of pleth variability index (PVI)-guided fluid therapy during abdominal surgery on tissue perfusion, 19 client-owned dogs that underwent elective abdominal surgery were randomized into control (n=10) and PVI (n=9) groups. In the control group, perioperative fluid management was based on the 2013 AAHA/AAFP Fluid Therapy Guidelines for Dogs and Cats. In the PVI group, the fluid rate was maintained based on basic daily requirements. If PVI was higher than 15% for >5min, 3–5mL/kg of crystalloid fluid bolus was infused. The tissue perfusion indicator, lactate levels, was measured at the time of intubation, extubation, and 6, 12 and 24h postoperatively. There were no significant differences in total and average fluid infused between control and PVI groups. The control group had significantly higher lactate levels than that of the PVI group at 12h postoperatively (2.2±1.0mmol/L versus 1.1±0.6mmol/L, p=0.008) and overall postoperatively (1.8±0.8mmol/L versus 1.2±0.5m...
为了评估腹部手术中体积变异性指数(PVI)引导的液体治疗对组织灌注的血流动力学优化效果，将19只接受择期腹部手术的客户犬随机分为对照组(n=10)和PVI组(n=9)。在对照组中，围手术期液体管理基于2013年AAHA/AAFP犬猫液体治疗指南。在PVI组，液体量维持在基本的每日需水量。若PVI高于15%持续>5min，则给予3-5mL /kg结晶液丸。分别于插管、拔管时及术后6、12、24h测定组织灌注指标乳酸水平。对照组和PVI组总输注量和平均输注量无显著差异。对照组术后12h乳酸水平显著高于PVI组(2.2±1.0mmol/L vs 1.1±0.6mmol/L, p=0.008)，术后总体(1.8±0.8mmol/L vs 1.2±0.5m)。
{"title":"PLETH VARIABILITY INDEX-GUIDED FLUID THERAPY LOWERS POST-OPERATIVE LACTATE LEVELS IN DOGS UNDERGOING ELECTIVE ABDOMINAL SURGERY: A RANDOMIZED CONTROLLED TRIAL","authors":"Chun-Hsiang Hu, T. Hsu, Kuan-Sheng Chen, Wei-Ming Lee, Hsien-Chi Wang","doi":"10.1142/S168264851750010X","DOIUrl":"https://doi.org/10.1142/S168264851750010X","url":null,"abstract":"To evaluate the hemodynamic optimization effect of pleth variability index (PVI)-guided fluid therapy during abdominal surgery on tissue perfusion, 19 client-owned dogs that underwent elective abdominal surgery were randomized into control (n=10) and PVI (n=9) groups. In the control group, perioperative fluid management was based on the 2013 AAHA/AAFP Fluid Therapy Guidelines for Dogs and Cats. In the PVI group, the fluid rate was maintained based on basic daily requirements. If PVI was higher than 15% for >5min, 3–5mL/kg of crystalloid fluid bolus was infused. The tissue perfusion indicator, lactate levels, was measured at the time of intubation, extubation, and 6, 12 and 24h postoperatively. There were no significant differences in total and average fluid infused between control and PVI groups. The control group had significantly higher lactate levels than that of the PVI group at 12h postoperatively (2.2±1.0mmol/L versus 1.1±0.6mmol/L, p=0.008) and overall postoperatively (1.8±0.8mmol/L versus 1.2±0.5m...","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"1 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90159768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-01DOI: 10.1142/S1682648517500093
Chai-Chi Liu, S. Ou, Dune-Huey Tan, M. Hsieh, P. Chang
The FlfA fimbrial protein is an important virulence factor and vaccine candidate of Avibacterium paragallinarum. Although all Taiwanese field strains of A. paragallinarumwere shown to contain an intact flfA gene, only some of them express the flfA protein in vitro. The aim of this study was to investigate how flfA expression is controlled in A. paragallinarum. The results of quantitative reverse transcription-polymerase chain reaction, 5′ rapid amplification of cDNA ends, and sequencing analyses showed that flfA expression is controlled by the length of a poly-cytidine (poly-C) tract that is located at the promoter region of flfA. In vitro passage of A. paragallinarum in chickens led to a change in the length of the poly-C tract and subsequently turned off flfA expression. These findings are valuable for understanding the mechanism of pathogenicity of A. paragallinarum.
{"title":"LENGTH OF POLY-CYTIDINE REPEATS CONTROLS THE PHASE-VARIABLE EXPRESSION OF THE FIMBRIAL PROTEIN IN AVIBACTERIUM PARAGALLINARUM","authors":"Chai-Chi Liu, S. Ou, Dune-Huey Tan, M. Hsieh, P. Chang","doi":"10.1142/S1682648517500093","DOIUrl":"https://doi.org/10.1142/S1682648517500093","url":null,"abstract":"The FlfA fimbrial protein is an important virulence factor and vaccine candidate of Avibacterium paragallinarum. Although all Taiwanese field strains of A. paragallinarumwere shown to contain an intact flfA gene, only some of them express the flfA protein in vitro. The aim of this study was to investigate how flfA expression is controlled in A. paragallinarum. The results of quantitative reverse transcription-polymerase chain reaction, 5′ rapid amplification of cDNA ends, and sequencing analyses showed that flfA expression is controlled by the length of a poly-cytidine (poly-C) tract that is located at the promoter region of flfA. In vitro passage of A. paragallinarum in chickens led to a change in the length of the poly-C tract and subsequently turned off flfA expression. These findings are valuable for understanding the mechanism of pathogenicity of A. paragallinarum.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"40 1","pages":"27-32"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90148653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-14DOI: 10.1142/S1682648517720039
Wei-Hsun Chao, J. Lai, Xiao-Xuan He, Yu-Hung Lin, Geng-Ruei Chang
Although infectious keratoconjunctivitis (IKC) has occurred worldwide, no report has mentioned an IKC case in a dairy goat farm in an indoor management system. Moreover, few clinicians follow the I...
{"title":"CASE REPORT: AN INFECTIOUS KERATOCONJUNCTIVITIS OUTBREAK CAUSED BY MYCOPLASMA CONJUNCTIVAE IN A DAIRY GOAT FARM","authors":"Wei-Hsun Chao, J. Lai, Xiao-Xuan He, Yu-Hung Lin, Geng-Ruei Chang","doi":"10.1142/S1682648517720039","DOIUrl":"https://doi.org/10.1142/S1682648517720039","url":null,"abstract":"Although infectious keratoconjunctivitis (IKC) has occurred worldwide, no report has mentioned an IKC case in a dairy goat farm in an indoor management system. Moreover, few clinicians follow the I...","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"os9 1","pages":"33-39"},"PeriodicalIF":0.0,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88403108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：