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PHOSPHATE-MOBILIZING MICROORGANISMS: 1. BIODIVERSITY, INFLUENCE ON PLANTS MINERAL NUTRITION AND PRODUCTIVITY 调动磷酸盐的微生物:生物多样性,对植物矿质营养和生产力的影响
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-4-28-159-182
L. Chaikovskaya, O. Ovsienko
Phosphorus is an essential plant nutrient involved in plants’ growth and development, accelerated formation of reproductive organs and other important factors for obtaining high and stable crop yields and, therefore, high-quality products. Easily absorbed phosphorus compounds obtained from soil or fertilizers are the most valuable for plants. One of the promising directions for improving the phosphorus nutrition of agricultural crops is biological phosphate mobilization that is carried out by soil microorganisms – bacteria and filamentous fungi (micromycetes). They contribute to the conversion of poorly soluble phosphorus compounds into forms accessible to higher plants. The effect of microorganisms on plants in terms of improving their mineral nutrition, in particular phosphorus, was reviewed in this article. We analysed domestic and foreign literature sources (over the last 15 years) focused on the biodiversity of soil phosphate-mobilizing microorganisms and their influence on converting insoluble phosphate to soluble forms. The features of the mechanisms of biotransformation of organic and mineral phosphates by soil microorganisms and the main criteria for screening effective isolates able to convert poorly soluble phosphorus compounds into forms available for plants were described in detail. This review also demonstrates that several microorganisms belonging to different taxonomic groups (bacteria and micromycetes) are recognized as powerful phosphate solubilizers or bioconverters of poorly soluble phosphorus compounds into water-soluble forms. We also surveyed scientific works, in which the practical application of effective strains of microorganisms that transform unavailable phosphorus compounds into available for plants was studied; and those, in which the role of microorganisms in increasing the availability of phosphorus for agricultural plants and their productivity improvement was demonstrated. A comprehensive description of microbial preparations “Agrofil”, “Biovays”, “Ekophosphorin”, “Ekovital”, “Biophosphorin”, “Albobacterin”, “Polymiksobacterin”, “Agrobacterin”, “Phosphostim”, “Fitostimophos”, “Agromik”, “Baktopin” based on phosphate-mobilizing microorganisms, developed and used in various countries to optimize mineral nutrition of cultivated plants, is given.
磷是植物必需的营养物质,参与植物的生长发育、加速生殖器官的形成等,是作物获得高产、稳产、优质产品的重要因素。从土壤或肥料中获得的易于吸收的磷化合物对植物最有价值。利用土壤微生物-细菌和丝状真菌(微菌)进行生物磷化是改善农作物磷营养的一个有希望的方向。它们有助于将难溶性磷化合物转化为高等植物可获得的形式。本文综述了微生物在改善植物矿质营养,特别是磷营养方面的作用。我们分析了国内外文献来源(过去15年),重点关注土壤磷酸盐动员微生物的生物多样性及其对将不溶性磷酸盐转化为可溶性磷酸盐的影响。详细介绍了土壤微生物转化有机磷和无机磷的机理特点,以及筛选能将难溶性磷化合物转化为植物可利用形态的有效分离物的主要标准。这篇综述还表明,属于不同分类群的几种微生物(细菌和微菌类)被认为是强大的磷酸盐增溶剂或将难溶性磷化合物转化为水溶性形式的生物转换器。我们还调查了科学工作,其中研究了将不可用磷化合物转化为植物可利用的有效微生物菌株的实际应用;其中微生物在提高农业植物磷素利用率和提高生产力方面的作用得到了证明。全面介绍了各国为优化栽培植物矿质营养而开发和使用的以磷酸盐动员微生物为基础的微生物制剂“Agrofil”、“Biovays”、“Ekophosphorin”、“Ekovital”、“Biophosphorin”、“Albobacterin”、“polymisobacterin”、“Agrobacterin”、“Phosphostim”、“Fitostimophos”、“Agromik”、“Baktopin”。
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引用次数: 3
PRODUCTIVITY OF GREEN MANURE CROPS DEPENDING ON HYDROTHERMAL CONDITIONS 绿肥作物的生产力取决于水热条件
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-144-154
A. Prikhodko, A. Cherkashyna
Green manuring can increase biological activity of the soil, enrich it with organic matter, nutrients and improve its agrophysical and agrochemical properties. The aim of the research was to establish the influence of green manure crops species composition on the productivity of agrophytocenoses and the input of soil organic matter under various hydrothermal conditions. The studies were conducted in 2016–2019 on the experimental fields of the Research Institute of Agriculture of Crimea. Soil – chernozems southern low-humus. Average daily air temperature was: in 2016 – 11.8 °С; in 2017 – 12.7 °С; in 2018 – 12.5 °С; in 2019 – 12.6 °С (average long-term values – 10.8 °С). The amount of precipitation over the years was at the level of 162, 67, 129, 108 % of the norm. The experiments were laid according to B. A. Dospekhov “Methods of field research”. Position of the variants is systematic, triple replication. Fields square – 720 m2. The research subject is green manure crops agrocenoses. The object of the research is the process of forming the productivity of green manure crops. On average, over the years of research, the highest yield of green mass was formed by sweet clover and sainfoin (29.1 and 27.1 t/ha, respectively), winter triticale (24.5 t/ha) and winter rye (25.8 t/ha). T. pratense and P. tanacetifolia were low-yielding. The largest amount of accumulated soil organic matter was detected when rye, triticale, sainfoin and sweet clover were used as green manure; it was 5.88, 5.72, 5.56 and 5.52 t/ha, respectively. We established an average correlation (at 5 % significance level) between the amount of precipitation for the period “sowing – ripeness” in 2016–2017 and the yield of green mass of green manure crops (r = 0.52), as well as dry matter yield (r = 0.59). A negative correlation of average strength was also established between the average daily temperature of the period “sowing – ripeness” in 2017–2018 and yield of green mass (r = –0.66), as well as organic matter yield (r = –0.64).
绿色施肥可以提高土壤的生物活性,丰富土壤的有机质和养分,改善土壤的农业理化性质。本研究旨在探讨不同热液条件下绿肥作物种类组成对农用植物群落生产力及土壤有机质输入量的影响。这些研究于2016-2019年在克里米亚农业研究所的实验田进行。土壤-黑钙土南部低腐殖质。2016年日平均气温为- 11.8°С;2017年- 12.7°С;2018年- 12.5°С;2019年- 12.6°С(长期平均值- 10.8°С)。历年降水量分别为正常值的16.2%、67.0%、129%、108%。实验是根据B. A. Dospekhov的“实地研究方法”进行的。变异的位置是系统的,三重复制。场地平方- 720平方米。研究对象为绿肥作物。研究的对象是绿肥作物生产力的形成过程。平均而言,在多年的研究中,绿块产量最高的是甜三叶草和红豆(分别为29.1和27.1 t/ha)、冬季小黑麦(24.5 t/ha)和冬季黑麦(25.8 t/ha)。黄芪和黄芪产量较低。以黑麦、小黑麦、红豆和甜三叶草作为绿肥时,土壤有机质积累量最大;分别为5.88、5.72、5.56和5.52 t/ha。我们在2016-2017年“播种-成熟”期降水量与绿肥作物绿质量产量(r = 0.52)和干物质产量(r = 0.59)之间建立了平均相关性(5%显著性水平)。2017-2018年“播种-成熟”期日均温度与绿质产量(r = - 0.66)、有机质产量(r = - 0.64)均与平均强度呈负相关。
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引用次数: 0
DROUGHT TOLERANCE OF THE NEW SELF-POLLINATED LINES OF MAIZE AND THE METHODS OF ITS ESTIMATION 玉米自花新品系的耐旱性及其评价方法
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-95-106
G. Krivosheev, N. Shevchenko, A. S. Ignatiev
More than half of the maize crops in the Russian Federation are located in areas with insufficient and unstable moisture. With this in mind, Z. mays breeding for drought tolerance is extremely important. The purpose of the current study is: investigate the drought tolerance of self-pollinated maize lines by various methods, identify the best samples for further breeding, establish indirect criteria for estimating drought tolerance. The study was conducted at the Agricultural Research Center “Donskoy” located in the southern part of the Rostov region, which is characterized by unstable moisture. The years of the study turned out to be dry (Selyaninov Hydrothermal Coefficient (HTC) = 0.32–0.89). The new self-pollinated lines of maize were used as initial material and studied for resistance to water stress (41 pcs. in 2011–2013, 39 pcs. in 2015–2017, 25 pcs. in 2018–2020). According to the guidelines for maize breeding, the following indicators of productivity were taken into account: number of ears per plant, weight of one ear, 1000-grain weight, number of grains per ear, etc. In 2011–2013, based on the comprehensive estimation, new middle-early drought-tolerant self-pollinated lines of maize ‘DK47111’, ‘SP203’, ‘KS211’, ‘S204’ and ‘S238’ were identified. They were characterized by the low values of residual water deficit both in the flowering (6.9 – 12.8%) and milk-wax (9.0 – 13.4%) stage, high drought tolerance index (68.6 – 85.6%), deep root system (force of uprooting the plants from the ground was 54.0–67.7 kgf), minimum gap in the flowering of male and female inflorescences (1–2 days). In 2015–2017, according to the method of residual water deficit (RWD), the following lines were identified ‘KV334’, ‘RD6’, ‘DS498/217-4’, ‘DS257/85-3’, ‘DS257/85-1’, ‘DS257/85-6’, ‘DS257/85-4’. In 2018–2020, the new self-pollinated lines of maize ‘KS317A’, ‘KV240’, ‘LSh16’, ‘S86’, ‘LSh17’ and ‘LSh2’ showed high resistance to water stress. We recommend the following selection criteria when creating new lines: absence of infertility and kernel percentage of the ears.
俄罗斯联邦一半以上的玉米作物生长在水分不足和不稳定的地区。考虑到这一点,Z. mays的耐旱育种是极其重要的。本研究的目的是:通过各种方法研究自花授粉玉米品系的耐旱性,确定进一步选育的最佳样品,建立估计抗旱性的间接标准。这项研究是在位于罗斯托夫地区南部的农业研究中心“顿斯科伊”进行的,该地区的特点是湿度不稳定。研究的年份被证明是干燥的(Selyaninov热液系数(HTC) = 0.32-0.89)。以玉米自花授粉新品系为初始材料,对其抗水分胁迫能力进行了研究。2011-2013年,39件。2015-2017年,25件。在2018 - 2020年)。根据玉米育种指南,考虑了以下生产力指标:单株穗数、单穗重、千粒重、每穗粒数等。2011-2013年,在综合估算的基础上,鉴定出了玉米‘DK47111’、‘SP203’、‘KS211’、‘S204’和‘S238’中早抗旱自交新品系。其特点是花期(6.9 ~ 12.8%)和乳蜡期(9.0 ~ 13.4%)剩余水分亏缺值低,耐旱性指数高(68.6 ~ 85.6%),根系深(离地拔根力54.0 ~ 67.7 kgf),雌雄花序开花间隔最小(1 ~ 2 d)。2015-2017年,根据剩余水分亏缺(RWD)方法,鉴定出以下品系:KV334、RD6、DS498/217-4、DS257/85-3、DS257/85-1、DS257/85-6、DS257/85-4。2018-2020年,玉米自花新品系‘KS317A’、‘KV240’、‘LSh16’、‘S86’、‘LSh17’和‘LSh2’对水分胁迫表现出较高的抗性。在创建新品系时,我们推荐以下选择标准:无不育和穗粒百分比。
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引用次数: 0
BIOLOGY OF SEED GERMINATION IN SOME ONION SPECIES (ALLIUM L.) 某些洋葱(allium l .)种子萌发生物学研究
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-180-190
T. Fomina
The possibility of seed reproduction is one of the factors of successful cultivation of resource plants in specific environmental conditions. The aim of the work was to study the germination behavior, quality and longevity of seeds in 15 onions (Allium L.) from the collection of the Central Siberian Botanical Garden SB RAS, Novosibirsk. The research was carried out in 1996–2019. Laboratory germination of seeds was determined according to generally accepted methods. Seeds were tested 3–7 months after harvesting at 17–23 °C in the light. The number of seeds in each Petri dish – 25–50; double replication. In the case of hindered germination, a two-month chilling at 4 °C was applied. In the future, laboratory germination was determined after 3, 5 and 7 years of room storage. We have established that onion seeds have three types of germinating. Seeds of type I – eight species, one variety from the subgenus Rhizirideum – usually lack dormancy. Germination is fast and simultaneous, and average germination percentages are 77.1–92.2 %. Seeds of type II – A. leucocephalum and A. microdictyon from the subgenus Rhizirideum, also A. caeruleum and A. flavum from the subgenus Allium – are characterized by stretched germination period due to the shallow dormancy, and their germination percentages vary from 32.6 % to 81.1 %. Seeds of type III – A. obliquum from the subgenus Rhizirideum and two species of the subgenus Melanocrommyum – do not germinate in room conditions, or germinate with low germination percentages, whereas chilling increases them up to 47.0–67.0 %. The hindered germination of these seeds is due to the deep organic dormancy. The economic longevity of onion seeds is 3–5 years, and the biological longevity varies at interspecific level within 5–8 years. Thus, the species that produce plump and good germinating seeds suitable for long-term storage – A. altaicum, A. bidentatum, A. flavum, A. microdictyon, A. nutans, A. ramosum, A. rubens, A. schoenoprasum, A. senescens, A. senescens var. glaucum, and A. strictum – have the highest reproductive potential in culture.
种子繁殖的可能性是资源植物在特定环境条件下培育成功的因素之一。研究了新西伯利亚中央植物园收集的15种洋葱(Allium L.)种子的萌发行为、质量和寿命。该研究于1996年至2019年进行。实验室种子发芽率按普遍接受的方法测定。种子在收获后3-7个月在17-23°C的光照下进行测试。每个培养皿中种子数量- 25-50;复制的两倍。在发芽受阻的情况下,在4°C下冷却两个月。在未来,实验室发芽是在3年、5年和7年的室内储存后测定的。我们已经确定洋葱种子有三种发芽方式。I型种子- 8种,1个变种来自根属-通常缺乏休眠。萌发速度快,同时萌发,平均发芽率为77.1 - 92.2%。II型种子——根状茎亚属的A. leucocephalum和A. microdictyon,以及葱属的A. caeruleum和A. flavum——由于休眠较浅,萌发期较长,发芽率从32.6%到81.1%不等。III型的种子——来自根状茎亚属的A. obliquum和黑桫椤亚属的两个种——在室内条件下不发芽,或者发芽率很低,而在低温下发芽率可达47.0% - 67.0%。这些种子的发芽受阻是由于深层的有机休眠。洋葱种子的经济寿命为3 ~ 5年,生物寿命在5 ~ 8年之间存在种间差异。因此,产生饱满、发芽良好、适合长期储存的种子的品种——altaicum、A. bidentatum、A. flavum、A. microdictyon、A. nutans、A. ramosum、A. rubens、A. schoenoprasum、A. senescens、A. senescens变种glaucum和A. strictum——在培养中具有最高的繁殖潜力。
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引用次数: 0
EFFECT OF TEMPERATURE FACTOR ON THE DURATION OF INTERPHASE PERIODS OF MELON PLANTS 温度因子对甜瓜植株间期持续时间的影响
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-4-28-82-91
N. A. Eliseeva, Y. Kostanchuk
One of the valuable traits of any variety is its degree of adaptability to stressful environmental factors. The purpose of the research is to determine the degree of influence of average daily temperatures on the duration of interphase periods of some melon varieties in the first half of growing season to assess their ecological plasticity under conditions of the foothill zone of the Crimea. The studies were conducted in 2016–2019 on the experimental fields of FSBSI “Research Institute of Agriculture of Crimea” (village of Ukromnoye, Simferopol suburbs). All the research studies were carried out according to the existing guidelines for melon crops breeding. Fifty-six melon samples varying in reaching maturity were the objects of the study. Soil – chernozem southern calcareous heavy loamy; its texture is clayey, structure – lumpy. Weather conditions during the years of research varied. The most favourable temperature regime for the melon growing was in 2019 (2269.6 °С); unfavourable – in 2016 (1738.0 °С); 2017 and 2018 occupied an intermediate position. It allowed obtaining objective results in assessing ecological plasticity of the studied varieties. Planting scheme – standard; feeding area – 1.0×1.5 m2 per one plant. The study was carried out in a collection nursery; four-fold replication. We compared melons of three groups of ripeness: early (I) – varieties mature in 55–75 days, mid-early (II) – ripen in 76–90 days, mid-late (III) – require 90 days until harvest. During the years of research, the duration of interphase periods varied from 18 to 59 days. An increase in the relative average daily air temperature by 1 °C reduced the phenophases duration on average by 1.02–4.43 days. The most pronounced changes were revealed in the third interphase period (seedlings – fruit formation beginning): the average value of the ecological variation corresponded to 27.5 %; maximum it was in the mid-late samples (25.3 %). We established a close negative correlation (from –0.72 to –0.99) between an increase in the relative average daily air temperature and duration of the phenophases of the first half of the melon plants growing season. Each genotype reacted individually to changes in external influences. The group of mid-late samples had the highest degree of variability. An increase in the relative average daily temperatures by 1 °C reduces the length of the interphase periods of plants of this group from 1.99 to 3.75 days. Among others, varieties ‘Idyllia’, ‘Gulnara’, ‘Ethiopka’ were the most stable.
任何品种的一个有价值的特征是它对压力环境因素的适应程度。本研究的目的是确定在克里米亚山麓地区条件下,平均日气温对某些甜瓜品种生长季前半期间期持续时间的影响程度,以评估其生态可塑性。该研究于2016-2019年在FSBSI“克里米亚农业研究所”(辛菲罗波尔郊区Ukromnoye村)的实验田进行。所有的研究都是根据现有的甜瓜作物育种指南进行的。56个不同成熟度的甜瓜样本是这项研究的对象。土壤-黑钙土南部钙质重壤土;其质地粘稠,结构块状。研究期间的天气状况各不相同。最适宜甜瓜生长的温度是2019年(2269.6°С);不利- 2016年(1738.0°С);2017年和2018年处于中间位置。对所研究品种的生态可塑性进行了客观的评价。种植方案。标准;饲喂面积-每株1.0×1.5 m2。这项研究是在一个收集托儿所进行的;4倍复制。我们比较了三组成熟度的瓜:早(I) -品种成熟55-75天,中早(II) -成熟76-90天,中晚(III) -需要90天才能收获。在研究期间,间期持续时间从18天到59天不等。相对平均日气温每升高1℃,物候期持续时间平均减少1.02 ~ 4.43天。在第三间期(种苗-果实形成开始)变化最显著,生态变异的平均值为27.5%;在中后期样品中最高(25.3%)。结果表明,相对日平均气温的升高与甜瓜生长季前半期物候期持续时间呈负相关(-0.72 ~ -0.99)。每种基因型对外部影响的变化都有各自的反应。中后期样品组具有最高程度的可变性。相对平均日温度每升高1℃,该组植物间期长度从1.99天减少到3.75天。其中,品种“Idyllia”、“Gulnara”、“Ethiopka”是最稳定的。
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引用次数: 1
VARIETAL SPECIFICITY OF OPTO-BIOLOGICAL PROPERTIES AND PHYTOMETRIC PARAMETERS OF WINTER WHEAT CROPS 冬小麦作物光生物学特性和植物计量参数的品种特异性
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-47-60
Y. Gulyanov, N. Nikolaev, I. Yakovlev
Identification of varietal specificity of opto-biological properties of field crops is necessary for adequate interpretation of satellite images, rapid determination of phytometric parameters of crops, indication of the level of their development and justification of corrective technological solutions. The aim of the research was to evaluate the opto-biological properties and phytometric parameters of diverse biological varieties of winter wheat; identify their relationship and inter-variety variability in high-tech reference crops based on satellite and ground monitoring. Field studies were conducted in 2019-2020 on the site of competitive ecological variety testing laid on the experimental field of the Orenburg State Agrarian University in the zone of the southern steppes of the Orenburg Cis-Urals (Preduralye). During the study, we found that agrocenoses of different winter wheat varieties differ markedly in opto-biological properties expressed in normalized difference vegetation index (NDVI) values. Varieties ‘Grom’, ‘Don 95’, ‘Bystritsa’, ‘Spartak’, ‘Stanichnaya’ and ‘Sintetik’ were characterized by the lowest average NDVI values during the growing season (0.57-0.59). Higher NDVI values (0.67) were observed in the crops of ‘Zhemchuzhina Povolzhya’, ‘Kolos Orenburzhya’, ‘Orenburgskaya 105’, ‘Rifey’ and ‘Pionerskaya 32’ varieties. The variability between the varieties according to the lowest (0.51 – ‘Grom’, 0.52 – ‘Don 95’) and the highest (0.69 – ‘Kolos Orenburzhya’, 0.70 – ‘Pionerskaya 32’, 0.69 – ‘Orenburgskaya 105’) NDVI values during the maximum assimilation apparatus development reached 0.17-0.19 units (phase of full spring tillering). By the heading, when NDVI was 0.78–0.79 and 0.85 units, respectively, this difference somewhat leveled but, in general, remained significant – at the level of 0.08-0.11 units (10.9–14.9 %). The largest leaf area (30 thousand m2/ha) was typical for the crops of ‘Saratovskaya 17’, ‘Kolos Orenburzhya’, ‘Orenburgskaya 105’, ‘Rifey’ and ‘Pionerskaya 32’. There was no direct inter-variety relationship between the leaf area and NDVI of winter wheat crops (R 2= 0.38).
鉴定大田作物光生物学特性的品种特异性对于充分解释卫星图像、快速确定作物的植物测定参数、指示其发展水平和证明纠正技术解决方案是必要的。研究了不同冬小麦生物品种的光生物学特性和植物计量参数;在卫星和地面监测的基础上,确定它们之间的关系和高科技参考作物的品种间变异。2019-2020年,在奥伦堡-乌拉尔(Preduralye)南部草原地区奥伦堡州立农业大学实验田的竞争性生态品种试验场进行了实地研究。在研究中,我们发现不同冬小麦品种的草地在归一化植被指数(NDVI)值表达的光生物学特性上存在显著差异。品种‘Grom’、‘Don 95’、‘Bystritsa’、‘Spartak’、‘Stanichnaya’和‘Sintetik’的生长季平均NDVI值最低(0.57 ~ 0.59)。‘Zhemchuzhina Povolzhya’、‘Kolos Orenburzhya’、‘Orenburgskaya 105’、‘Rifey’和‘Pionerskaya 32’的NDVI值较高(0.67)。同化器最大发育阶段(春季分蘖期)NDVI值最低(0.51 - ' Grom ', 0.52 - ' Don 95 ')和最高(0.69 - ' Kolos Orenburzhya ', 0.70 - ' Pionerskaya 32 ', 0.69 - ' Orenburgskaya 105 '),品种间差异达0.17-0.19个单位。在标题中,当NDVI分别为0.78-0.79和0.85单位时,这种差异在一定程度上趋于平衡,但总体上仍然显著-在0.08-0.11单位的水平上(10.9 - 14.9%)。最大的叶面积(3万平方米/公顷)是典型的‘Saratovskaya 17’、‘Kolos Orenburzhya’、‘Orenburgskaya 105’、‘Rifey’和‘Pionerskaya 32’作物。冬小麦作物叶面积与NDVI无直接的品种间关系(r2 = 0.38)。
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引用次数: 0
BIORATIONAL METHOD OF AMBROSIA ARTEMISIIFOLIA L. GROWTH AND DEVELOPMENT INHIBITION 艾草生长发育抑制的生物实验方法
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-61-74
S. Didovich, A. N. Pas, I. Danilova, O. Alekseenko
Currently, the search for biological agents that are alternative to chemical ones to protect agrocenoses from weeds, including quarantine objects like different types of ragweed, is relevant because they cause biological and technological damage to the environment, agriculture and have a negative impact on human health. The research was aimed at searching for biotically active elicitors of plant and microbial origin for the induction, reinforce of oxidative stress and inhibition of Ambrosia artemisiifolia L. growth. Strains were searched for and studied in 2019–2020 in laboratory experiments and greenhouse trials in the Research Institute of Agriculture of Crimea. Ragweed plants were grown in pots. Soil – chernozem southern. In the phase of 4–6 leaves, plants were treated with biorational preparative forms based on microbial and plant elicitors at a dose of 200 mkl/plant. For bioherbicidal composites, strains-inhibitors from the Research Institute of Agriculture of Crimea collection (CCM), plant extracts from the ragweed and glycerin were used. The inhibition efficiency was evaluated three weeks after treatment. The indices of height, phytomass, antioxidant status and ragweed damage degree were taken into account. We identified seven strains that inhibited plant growth by 0.13–1.08 g/plant (37–38 %) compared to the control variants: 1) water treatment, 2) bacterization with a phytopathogenic strain of Stagonosporopsis heliopsidis from the All-Russian Scientific Research Institute of Plant Protection collection. For the first time, we used ambrosia BAS to develop a birational herbicide to control the ragweed. Bacterization with biorational preparative forms based on biotically active elicitors of plant and microbial origin affected the homeostasis of ragweed, induced plant stress by blocking the enzymatic activity and the antioxidant protection systems. The activity of catalases and polyphenoloxidases decreased by 2.9–85.6 and 1.2–658.0 times, respectively, with a significant correlation between themselves (r = 0.66) and the correlation of catalase activity with plant phytomass (r = 0.72). The content of glutathione decreased by 2.5–2.7 compared to the control and had significant correlations with the activity of catalases (r = 0.63) and the phytomass of ragweed (r = 0.80) (p < 0.05) depending on the components of the biohebicidal formulations.
目前,寻找替代化学制剂的生物制剂以保护农草不受杂草(包括不同类型的豚草等检疫对象)的侵害是有意义的,因为它们对环境和农业造成生物和技术损害,并对人类健康产生负面影响。本研究旨在寻找具有生物活性的植物和微生物源激发子,诱导、增强氧化应激,抑制艾草的生长。2019-2020年在克里米亚农业研究所进行实验室试验和温室试验,对菌株进行检索和研究。豚草种植在花盆里。土壤-黑钙土南部。在4-6叶期,用200 mkl/株剂量的微生物和植物激发剂制备生物制剂。生物除草复合材料采用克里米亚农业研究所收集的菌种抑制剂、豚草植物提取物和甘油。治疗3周后评价抑菌效果。考察了豚草株高、植物质量、抗氧化能力和豚草危害程度等指标。与对照菌株相比,我们发现7株菌株对植物生长的抑制作用为0.13-1.08 g/株(37 - 38%):1)水处理;2)用全俄植物保护科学研究所收集的一株植物病原菌株进行细菌处理。本研究首次利用安氏菌(ambrosia BAS)开发了一种防治豚草的双边除草剂。以植物和微生物源的生物活性激发子为基础的生物制剂形式进行杀菌,通过阻断豚草的酶活性和抗氧化保护系统,影响豚草的体内平衡,诱导植物应激。过氧化氢酶和多酚氧化酶活性分别降低了2.9 ~ 85.6倍和1.2 ~ 658.0倍,两者之间呈显著相关(r = 0.66),过氧化氢酶活性与植物生物量呈显著相关(r = 0.72)。谷胱甘肽含量与豚草过氧化氢酶活性(r = 0.63)和植物质量(r = 0.80)呈显著相关(p < 0.05),且不同成分的杀草制剂中谷胱甘肽含量比对照降低了2.5 ~ 2.7。
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引用次数: 0
BALANCE OF NUTRITION ELEMENTS IN CROP ROTATION UNDER BIOLOGIZED ADAPTIVE LANDSCAPE FARMING 生物适应性景观耕作轮作中营养元素的平衡
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-84-94
L. Kozlova, E. N. Noskova, F. Popov, E. Svetlakova
In the light of recent developments, the use of biological techniques in crop cultivation technologies needs to be expanded. The role of crop rotations with a high proportion of legume grasses, green manure crops and intermediate crops is increasing. The purpose of the research is to study soil fertility dynamics in terms of the main nutritional elements and determine their balance for the development of the adaptive landscape farming system in the North-Eastern region of the European part of Russia. Experiments were carried out on the experimental plots of the FARC of the North-East in 2002–2009. Soil – sod-podzolic middle loamy. We analyzed data of a long-term stationary experiment in four grain-fallow-grass field crop rotations. Meteorological conditions during the years of research were close to the average long-term values and favorable for crop cultivation. The introduction of low doses of fertilizers (N45P45K45 as mineral, 11.5–22.5 t/ha as green manure) increased the content of mobile phosphorus in the soil by 11–14 mg/kg (LSD05 = 9.5) in crop rotations with occupied and green manure fallows. An increase in exchangeable potassium by 15 mg/kg (LSD05 = 14.1) was noted in crop rotation with two clover fields for green manure. A positive balance of nitrogen and phosphorus was obtained in crop rotation with occupied and green manure fallows. The nitrogen balance was with a higher intake of +157.8 – +483.5 kg/ha and balance intensity – 115–140 %. The balance of phosphorus has developed at +6.0…+49.6 kg/ha. The positive balance of potassium was only in crop rotations with the introduction of green manure fertilizers and intermediate crop (+69.9…+175.8 kg/ha). The intensity of the balance of these elements was 101–118 %. In the control crop rotation with bare fallow at a minimum intake of nutrients, the negative balance of nitrogen, phosphorus and potassium was obtained; the intensity was below 100 %. Productivity of crop rotation was at the level of 4.90–5.41 thousand fodder units. According to the optimal combination of productivity and the balance of nutritional elements, crop rotation with two clover fields for green manure and an intermediate crop stood out.
鉴于最近的发展,生物技术在作物栽培技术中的应用需要扩大。豆科牧草、绿肥作物和中间作物轮作比例较高,轮作的作用越来越大。本研究的目的是研究俄罗斯欧洲部分东北地区主要营养元素的土壤肥力动态,并确定其平衡,为适应性景观耕作系统的发展提供依据。2002-2009年,在哥伦比亚革命武装力量东北部的试验田进行了实验。土壤-草灰化中壤土。我们分析了四种谷休草轮作的长期平稳试验数据。研究年份的气象条件接近长期平均值,有利于作物种植。施用低剂量肥料(N45P45K45为矿物肥,11.5 ~ 22.5 t/ hm2为绿肥)可使占用绿肥休耕地的轮作土壤中流动磷含量提高11 ~ 14 mg/kg (LSD05 = 9.5)。两个三叶草田轮作绿肥时,交换态钾增加了15 mg/kg (LSD05 = 14.1)。利用绿肥休耕和占用耕地轮作,氮磷平衡达到正平衡。氮素平衡较高,采食量为+157.8 ~ +483.5 kg/ha,平衡强度为- 115 ~ 140%。磷平衡发展到+6.0 ~ +49.6 kg/ha。钾肥的正平衡仅在绿肥和中间作物轮作中出现(+69.9 ~ +175.8 kg/ha)。这些元素的平衡强度为101 ~ 118%。在最低养分摄取量下的对照轮作中,氮、磷、钾达到负平衡;强度低于100%。轮作产量在4.90 ~ 5.41万饲料单位水平。根据生产力的最优组合和营养元素的平衡,两片三叶草轮作绿肥田和一种中间作物轮作较为突出。
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引用次数: 1
SOURCES OF HIGH-QUALITY GRAIN IN WINTER COMMON WHEAT BREEDING 优质籽粒在冬小麦育种中的来源
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-3-27-164-171
N. Sokolenko, N. Galushko, N. M. Komarov
Research on improving winter common wheat varieties in the context of combining high yield and grain quality is relevant. The research aimed to evaluate and select variety samples of winter common wheat with high technological indicators of grain quality for breeding purposes. In this study, we analyzed grain from 10 variety samples of the world collection; winter wheat grain of variety ‘Aivina’ served as a standard. Soil – ordinary medium-loamy medium-thick low-humus chernozem. Wheat was grown on the experimental fields of the North Caucasus FARC in the zone with unstable moistening on bare fallow. Grain vitreousness percentage in 2019 ranged between 44.0 and 53.0 %, in 2020 – 30.5–50.0 %. In the same years, this indicator for variety-standard ‘Aivina’ was 56.0 and 48.0 %, respectively. The gluten content in the grain depended on the weather conditions and genotype. Grain of all varieties harvested in 2019, including standard ‘Aivina’, had a high gluten content (28.2–35.6 %) and corresponded to strong wheat. In 2020, the situation was absolutely different. The amount of gluten in the grain of variety samples harvested in 2020 ranged between 22.9 and 34.6 %; for ‘Aivina’, this indicator reached 26.9 %. The gluten quality in all variety samples during the years of research was typical for class 2. The variety samples were characterized by a high protein content in the grain – 16.7–20.8 % and 13.5–20.4 %; the same indicator for ‘Aivina’ was 16.7 and 15.8 %. Sedimentation rate in the variety samples was 50–81 ml and 34–64 ml; in ‘Aivina’ – 50 and 43 ml. Maximum sedimentation values were noted for the variety ‘Prestizh’. It indicates the ability to form grain, the strength of which is typical for wheat-improver. The correlation between sedimentation and gluten content (r = 0.63), as well as between sedimentation and the falling number (r = 0.73), was positive and significant during the years of research. All studied genotypes are promising for breeding new wheat varieties as sources for obtaining high-quality grain.
在高产与品质相结合的背景下,对冬小麦品种进行改良研究具有重要意义。本研究旨在对籽粒品质技术指标较高的冬小麦品种样本进行评价和选育。在这项研究中,我们分析了世界上10个品种的谷物样本;品种“Aivina”的冬小麦籽粒作为标准。土壤-普通中壤土中厚低腐殖质黑钙土。小麦是在北高加索地区FARC的试验田种植的,该试验田在光秃秃的休耕地里水分不稳定。2019年谷物玻璃化率为44.0% ~ 53.0%,2020年为30.5 ~ 50.0%。同年品种标准‘艾维娜’的这一指标分别为56.0%和48.0%。籽粒中面筋含量受气候条件和基因型的影响。2019年收获的所有品种的谷物,包括标准的“Aivina”,麸质含量高(28.2 - 35.6%),相当于强小麦。2020年,情况完全不同。在2020年收获的品种样本中,谷蛋白含量在22.9%至34.6%之间;对于“Aivina”,这一指标达到26.9%。在研究期间,所有品种样品的面筋质量均为典型的2类。该品种籽粒蛋白质含量较高,分别为16.7 ~ 20.8%和13.5 ~ 20.4%;“Aivina”的相同指标为16.7%和15.8%。各品种样品的沉降率分别为50 ~ 81 ml和34 ~ 64 ml;在“Aivina”中- 50和43毫升。“Prestizh”品种的最大沉降值被记录下来。它表示形成颗粒的能力,这种强度是小麦改良剂的典型特征。在多年的研究中,沉降与面筋含量(r = 0.63)、沉降与下降数(r = 0.73)呈显著正相关。所研究的所有基因型都有希望作为优质小麦的来源来选育小麦新品种。
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引用次数: 0
VARIETY-SPECIFIC FEATURES OF BARLEY AT DIFFERENT STAGES OF ONTOGENESIS 大麦个体发生不同阶段的品种特异性特征
Pub Date : 2021-01-01 DOI: 10.33952/2542-0720-2021-4-28-129-137
L. V. Osipova, T. L. Kurnosova, I. A. Bykovskaya
In modern technologies of growing grain crops, high priority is given to the choice of varieties with significant adaptive potential and the development of ways to increase the resistance of plants to the action of various stresses. The purpose of our research was to identify the specific features of different varieties of barley during critical periods of growth. The studies were carried out in 2020–2021. The objects of research were varieties of spring barley created in the Moscow Research Institute of Agriculture. A series of laboratory and vegetative experiments were conducted, in the course of which we studied the indicators of the physiological status and varietal specificity of the resistance of spring barley to oxidative stress induced by an osmotically active sucrose solution during de-etiolation (laboratory experiments) and caused by soil drought at the VI stage of organogenesis (vegetative experiment, varieties ‘Vladimir’ and ‘Raushan’). The biometric indicators of sprouts and indicators of water status were evaluated. In all experiments, the varieties responded to stress by increasing the content of the oxidative stress marker malondialdehyde (MDA). Weak resistance of vegetative organs was noted in 1) roots – variety ‘Raushan’ (3.8 under stress conditions vs. 0.92 μM/g of raw mass under optimal conditions). ‘Moskovsky 2’ (8.1 vs.2.0), ‘Elf’ (4.5 vs. 1.38); 2) sprouts – ‘Raushan’ (4.6 vs.1.54), ‘Elf’ (5.0 vs. 1.37), ‘Nur’ (6.0 vs. 2.4). Varieties ‘Moskovsky 86’ and ‘Vladimir’ were the most resistant to stress since the accumulation of MDA increased to a lesser extent compared to its base content (‘Moskovsky 86’ – sprouts: 5.5 vs. 2.8; roots: 3.0 vs. 2.2 μM/g of raw mass; ‘Vladimir’ – sprouts: 3.4 vs. 2.9; roots: 3.9 vs.3.2 μM/g). Moreover, the content of chlorophylls a, b and carotenoids in varieties ‘Moskovsky 86’ and ‘Vladimir’ decreased respectively by 5.1; 5,9; 6.7 % and 8.0; 17,4; 6.9 % compared to the control options. Thus, variety ‘Vladimir’ is more stress-resistant than the other ones.
在现代粮食作物种植技术中,选择具有显著适应潜力的品种和开发提高植物对各种胁迫的抗性的方法是十分重要的。我们研究的目的是确定不同品种大麦在生长关键期的具体特征。这些研究是在2020-2021年进行的。研究对象是莫斯科农业研究所培育的春大麦品种。通过一系列室内和无性试验,研究了春大麦去黄化过程中渗透活性蔗糖溶液诱导的氧化应激(室内试验)和器官发生第6期土壤干旱引起的氧化应激(无性试验,品种‘Vladimir’和‘roushan’)的生理状态和品种特异性指标。对芽的生物特征指标和水分状况指标进行了评价。在所有试验中,这些品种都通过增加氧化应激标志物丙二醛(MDA)的含量来应对胁迫。1)根系品种‘若山’的营养器官抗性较弱(胁迫条件下为3.8 μM/g,最优条件下为0.92 μM/g)。《Moskovsky 2》(8.1 vs.2.0),“精灵”(4.5和1.38);2)芽——“Raushan”(4.6 vs.1.54),“精灵”(5.0和1.37),努尔(6.0和2.4)。品种‘Moskovsky 86’和‘Vladimir’对胁迫的抵抗力最强,因为MDA的积累比其基础含量增加的程度要小(‘Moskovsky 86’-芽:5.5比2.8;根:3.0 vs. 2.2 μM/g原料质量;“弗拉基米尔”——豆芽:3.4 vs 2.9;根:3.9 vs.3.2 μM/g)。品种Moskovsky 86和Vladimir的叶绿素a、b和类胡萝卜素含量分别下降了5.1个百分点;5、9;6.7%和8.0;17日4;与对照组相比,减少了6.9%。因此,品种“弗拉基米尔”比其他品种更抗压力。
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