Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-127-144
N. Kruglova, O. Seldimirova, A. Zinatullina
Drought is an unfavorable combination of meteorological conditions when plants experience a long-term water deficiency both in the air and soil. This is one of the most common abiotic stressors, which leads not only to significant crop losses but also rises threat to food security. Researchers are actively developing ways to breed drought-tolerant cultivars of economically valuable crops, especially cereals – the main food resource. One of the promising areas of biotechnological evaluation of the resistance of existing and newly created cereal genotypes to drought for breeding purposes is the use of culture in vitro. In this case, embryos at the particular stage of development are used as explants (so-called embryo culture in vitro). The review aims to analyze the literature and own data on the production of cereal regenerants in embryo culture in vitro under selective experimental conditions of imitation of physiological drought. It has been shown that in vitro cultivation of immature embryos at a critical stage of relative autonomy is especially promising. This kind of embryo does not depend on the physiological factors of the maternal organism and can autonomously give rise to the fully developed plant under adequate conditions in vitro and later ex vitro. This allows the biotechnologist to obtain regenerants directly, excluding an additional time-consuming stage of the formation of morphogenic calli in vitro. As follows, the time required for expensive experiments is also reduced. Data on the identification of the critical stage of the relative autonomy of the cereal embryogenesis are presented. Criterion (proposed by the authors) for identifying this stage by the ability of the embryos to complete embryogenesis and form the seedlings on a hormone-free medium in vitro and give rise to the full developed regenerants ex vitro has been analyzed. Furthermore, the analysis of the laboratory germination of the obtained caryopses was carried out. It was discovered that in spring soft wheat, for example, such stage, corresponding to the formation of all organs in the embryo, occurs 15 days after pollination. The issues of using relatively autonomous embryos in the biotechnological assessment of the genotype drought resistance under selective conditions in vitro are considered.
{"title":"Embryo culture in vitro in the experimental evaluation of drought resistance in cereals (review)","authors":"N. Kruglova, O. Seldimirova, A. Zinatullina","doi":"10.33952/2542-0720-2021-2-26-127-144","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-127-144","url":null,"abstract":"Drought is an unfavorable combination of meteorological conditions when plants experience a long-term water deficiency both in the air and soil. This is one of the most common abiotic stressors, which leads not only to significant crop losses but also rises threat to food security. Researchers are actively developing ways to breed drought-tolerant cultivars of economically valuable crops, especially cereals – the main food resource. One of the promising areas of biotechnological evaluation of the resistance of existing and newly created cereal genotypes to drought for breeding purposes is the use of culture in vitro. In this case, embryos at the particular stage of development are used as explants (so-called embryo culture in vitro). The review aims to analyze the literature and own data on the production of cereal regenerants in embryo culture in vitro under selective experimental conditions of imitation of physiological drought. It has been shown that in vitro cultivation of immature embryos at a critical stage of relative autonomy is especially promising. This kind of embryo does not depend on the physiological factors of the maternal organism and can autonomously give rise to the fully developed plant under adequate conditions in vitro and later ex vitro. This allows the biotechnologist to obtain regenerants directly, excluding an additional time-consuming stage of the formation of morphogenic calli in vitro. As follows, the time required for expensive experiments is also reduced. Data on the identification of the critical stage of the relative autonomy of the cereal embryogenesis are presented. Criterion (proposed by the authors) for identifying this stage by the ability of the embryos to complete embryogenesis and form the seedlings on a hormone-free medium in vitro and give rise to the full developed regenerants ex vitro has been analyzed. Furthermore, the analysis of the laboratory germination of the obtained caryopses was carried out. It was discovered that in spring soft wheat, for example, such stage, corresponding to the formation of all organs in the embryo, occurs 15 days after pollination. The issues of using relatively autonomous embryos in the biotechnological assessment of the genotype drought resistance under selective conditions in vitro are considered.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79332186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-28-40
Z. Akhtyamova, T. Arkhipova, E. Martynenko, T. Nuzhnaya, R. Ivanov, L. Kuzmina
The ability to produce phytohormones and influence their metabolism in plants is an important property of rhizosphere bacteria that determines their plant growth promoting effect. Since abscisic acid (ABA) reduces stomatal conductance and increases the ability of tissues to conduct water, maintenance of water balance in lettuce plants on the background of activation of their growth was associated with the accumulation of ABA under the influence of bacteria. The aim of the study is to test the hypothesis that the growth-stimulating effect of bacteria on plants depends on their ability to synthesize the hormone ABA. The plants were grown on a light platform; seedlings were treated with a bacterial suspension simultaneously with planting. The ABA content, the relative water content, the chlorophyll content, the level of non-photochemical quenching, the leaf area and the weight of the shoots were measured. The level of transcripts of the HvNCED1, HvNCED2, and HvCYP707A1 genes responsible for ABA metabolism in barley was assessed using real-time PCR. Comparison of the ABA-deficient mutant of barley and plants of its wild type revealed the stimulation of the growth of plants of both genotypes upon bacterial treatment. The shoot mass and leaf area of the untreated mutant with bacteria were about 30 % less compared to Steptoe. The stimulating effect of bacteria was manifested in an increase in leaf area by 15 % in Steptoe and by 35 % in Az 34; shoot mass – by 18 % and 41 %, respectively. As a result, the phenotype difference between plants of two genotypes decreased. In the deficient mutant, the ABA level increased under the influence of Bacillus subtilis IB-22 more than twice. It was due to the ability of bacteria to produce ABA and reduce the activity of ABA degradation in barley plants. The results obtained in this study indicate that certain bacterial strains are able to increase the level of ABA in plants, compensating for the genetically determined deficiency of this hormone.
{"title":"Influence of Bacillus subtilis strain on abscisic acid content in ABA-deficient barley mutant and its wild type","authors":"Z. Akhtyamova, T. Arkhipova, E. Martynenko, T. Nuzhnaya, R. Ivanov, L. Kuzmina","doi":"10.33952/2542-0720-2021-2-26-28-40","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-28-40","url":null,"abstract":"The ability to produce phytohormones and influence their metabolism in plants is an important property of rhizosphere bacteria that determines their plant growth promoting effect. Since abscisic acid (ABA) reduces stomatal conductance and increases the ability of tissues to conduct water, maintenance of water balance in lettuce plants on the background of activation of their growth was associated with the accumulation of ABA under the influence of bacteria. The aim of the study is to test the hypothesis that the growth-stimulating effect of bacteria on plants depends on their ability to synthesize the hormone ABA. The plants were grown on a light platform; seedlings were treated with a bacterial suspension simultaneously with planting. The ABA content, the relative water content, the chlorophyll content, the level of non-photochemical quenching, the leaf area and the weight of the shoots were measured. The level of transcripts of the HvNCED1, HvNCED2, and HvCYP707A1 genes responsible for ABA metabolism in barley was assessed using real-time PCR. Comparison of the ABA-deficient mutant of barley and plants of its wild type revealed the stimulation of the growth of plants of both genotypes upon bacterial treatment. The shoot mass and leaf area of the untreated mutant with bacteria were about 30 % less compared to Steptoe. The stimulating effect of bacteria was manifested in an increase in leaf area by 15 % in Steptoe and by 35 % in Az 34; shoot mass – by 18 % and 41 %, respectively. As a result, the phenotype difference between plants of two genotypes decreased. In the deficient mutant, the ABA level increased under the influence of Bacillus subtilis IB-22 more than twice. It was due to the ability of bacteria to produce ABA and reduce the activity of ABA degradation in barley plants. The results obtained in this study indicate that certain bacterial strains are able to increase the level of ABA in plants, compensating for the genetically determined deficiency of this hormone.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85321303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-208-222
I. Stavtzeva, N. Yegorova
Clary sage (Salvia sclarea L.) is a widely demanded essential oil plant. The products of its processing are used in medicine, perfumery and cosmetics, food industry, paint and varnish production. The main breeding tasks are to develop cultivars that combine high yield and quality of essential oil, resistant to abiotic and biotic stresses. All clary sage cultivars registered in the ‘State register of breeding achievements allowed for use’ Russian Federation were obtained on the basis of traditional methods. The initial breeding material of S. sclarea was obtained at the FSBSI “Research Institute of Agriculture of Crimea” using methods of cell engineering (induction of somaclones from callus cultures, obtaining hybrids in embryoculture and cell selection for resistance to osmotic stress). The aim of this work was to study the main economic and morphometric parameters of clary sage samples created using various biotechnological methods at the main stages of the breeding process. As a material for research, we used samples obtained in vitro using three biotechnological methods – regenerants (R2-R3) from calli of ‘C-785’ and ‘Taigan’ cultivars, obtained in isolated embryo culture hybrids (Salvia sclarea L. x S. grandiflora Etling.) and forms selected in embryoculture on a selective medium with an osmotic (mannitol or NaCl). When studying 10 sage samples (seed progeny of regenerants) in the breeding nursery (2012–2014), three that exceeded the control cultivar ‘Taigan’ in terms of basic economic characteristics were identified. The collection of essential oil from them was 1.2-1.4 times higher than in the control. In the nursery of competitive variety trials (2016–2019), when studying three samples (No. R3-1-6, 226-08 and 1-01), No. R3-1-6 was selected for a number of indicators. This sample is seed progeny of regenerant obtained by the induction of morphogenesis from callus. A characteristic feature of this sample was an increase in the number of lateral shoots per inflorescence by 41.1 %, which contributed to an increase in the mass of inflorescences. By the yield of inflorescences (162.0 c/ha) and the collection of essential oil (51.5 kg/ha), No. R3-1-6 exceeded control by 24.9 % and 43.9 %, respectively. The patent on the new clary sage cultivar ‘Selinzh’ (No. R3-1-6), in the creation of which the method of cell engineering was used for the first time, was received.
鼠尾草(Salvia sclarea L.)是一种被广泛需求的精油植物。其加工的产品用于医药、香水和化妆品、食品工业、油漆和清漆生产。育种的主要任务是培育高产、优质、抗非生物和生物胁迫的精油品种。在俄罗斯联邦“允许使用的国家育种成果登记册”中登记的所有鼠尾草品种都是根据传统方法获得的。在FSBSI“克里米亚农业研究所”使用细胞工程方法(从愈伤组织培养中诱导体细胞无性系，在胚胎培养中获得杂种，并选择抗渗透胁迫的细胞)获得了S. sclarea的初始育种材料。这项工作的目的是研究鼠尾草样品的主要经济和形态参数，在育种过程的主要阶段使用各种生物技术方法创建。作为研究材料，我们使用了三种生物技术方法获得的离体样品:‘C-785’和‘泰干’品种的愈伤组织再生体(R2-R3)，分离胚胎培养杂种(Salvia sclarea L. x S. grandflora Etling.)，以及在具有渗透(甘露醇或NaCl)的选择性培养基上选择的胚胎培养形式。通过对2012-2014年苗圃10份鼠尾草(再生种子后代)样本的研究，鉴定出3份鼠尾草在基本经济性状上优于对照品种‘太干’。精油的提取量是对照组的1.2 ~ 1.4倍。在竞品种试验苗圃(2016-2019)中，研究了3个样品(No.;R3-1-6, 226-08和1-01)，在一些指标中选择了R3-1-6。本样品为愈伤组织形态发生诱导再生体的种子后代。该样品的一个特征是每个花序的侧芽数增加了41.1%，这有助于增加花序的质量。通过花序产量(162.0 c/ha)和精油收集量(51.5 kg/ha)，R3-1-6分别超出对照24.9%和43.9%。鼠尾草新品种“赛林芝”(Selinzh)申请专利号:R3-1-6)是第一次使用细胞工程方法创建的细胞。
{"title":"Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process","authors":"I. Stavtzeva, N. Yegorova","doi":"10.33952/2542-0720-2021-2-26-208-222","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-208-222","url":null,"abstract":"Clary sage (Salvia sclarea L.) is a widely demanded essential oil plant. The products of its processing are used in medicine, perfumery and cosmetics, food industry, paint and varnish production. The main breeding tasks are to develop cultivars that combine high yield and quality of essential oil, resistant to abiotic and biotic stresses. All clary sage cultivars registered in the ‘State register of breeding achievements allowed for use’ Russian Federation were obtained on the basis of traditional methods. The initial breeding material of S. sclarea was obtained at the FSBSI “Research Institute of Agriculture of Crimea” using methods of cell engineering (induction of somaclones from callus cultures, obtaining hybrids in embryoculture and cell selection for resistance to osmotic stress). The aim of this work was to study the main economic and morphometric parameters of clary sage samples created using various biotechnological methods at the main stages of the breeding process. As a material for research, we used samples obtained in vitro using three biotechnological methods – regenerants (R2-R3) from calli of ‘C-785’ and ‘Taigan’ cultivars, obtained in isolated embryo culture hybrids (Salvia sclarea L. x S. grandiflora Etling.) and forms selected in embryoculture on a selective medium with an osmotic (mannitol or NaCl). When studying 10 sage samples (seed progeny of regenerants) in the breeding nursery (2012–2014), three that exceeded the control cultivar ‘Taigan’ in terms of basic economic characteristics were identified. The collection of essential oil from them was 1.2-1.4 times higher than in the control. In the nursery of competitive variety trials (2016–2019), when studying three samples (No. R3-1-6, 226-08 and 1-01), No. R3-1-6 was selected for a number of indicators. This sample is seed progeny of regenerant obtained by the induction of morphogenesis from callus. A characteristic feature of this sample was an increase in the number of lateral shoots per inflorescence by 41.1 %, which contributed to an increase in the mass of inflorescences. By the yield of inflorescences (162.0 c/ha) and the collection of essential oil (51.5 kg/ha), No. R3-1-6 exceeded control by 24.9 % and 43.9 %, respectively. The patent on the new clary sage cultivar ‘Selinzh’ (No. R3-1-6), in the creation of which the method of cell engineering was used for the first time, was received.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78098589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-109-115
S. V. Kosenko
Penza region is characterized by a diversity of natural and climatic zones and considerable variability of meteorological factors over the years. Therefore, the creation of high-yielding winter soft wheat varieties with high protein content in grain is one of the most significant challenges in this crop breeding. The purposes of this research are twofold: assess the ability of winter wheat varieties to form high-protein grain in various environmental conditions and isolate a valuable source material for breeding. The research objective is to evaluate winter wheat varieties by such indicator as “mass fraction of protein in grain”. The research was carried out from 2008 to 2017 in a forest-steppe zone of the Penza region. The soils of the experimental field – medium-humus medium-thick leached chernozem with the 35–40 cm depth of the plowing horizon. Ninety varieties of winter wheat from the world collection were the material for the research. The forecrop is black fallow. Square of experimental fields is 3 m2, triple replication. Seeding rate – 5.5 million germinating grains/ha. Standard – winter soft wheat variety ‘Fotinya’. Mass fraction of protein in grain in early ripening and mid-early varieties of winter wheat significantly differed from those of the mid-maturing group. Winter wheat varieties from the mid-ripening group were distinguished by a high protein content (on average 15.5 %), high yield (on average 3.8 t/ha), high yield of protein per hectare (on average 0.59 t/ha). According to the level of stress resistance, varieties ‘Fotinya’ and ‘Moskovskaya 39’ stood out (0.7 and 0.6, respectively). The following varieties are characterized by high genetic flexibility (15.3–16.2): ‘Fotinya’ (Penza region), ‘Moskovskaya 39’ (Moscow region), ‘Bezenchukskaya 380’ (Samara region), ‘Badulinka’ (Volgograd region), ‘Ershovskaya 25’ (Saratov region), ‘Zimorodok’, ‘Pobeda 50’ (Krasnodar Territory), ‘Zernogradka 8’, ‘Konkurent’ (Rostov Region). All the aforementioned varieties have less variability of a characteristic over the years (coefficient of variation – 3.2–9.7 %) and are recommended for use as a starting material for creating new forms.
{"title":"ОЦЕНКА СОРТОВ ОЗИМОЙ ПШЕНИЦЫ ПО ПРИЗНАКУ «МАССОВАЯ ДОЛЯ БЕЛКА В ЗЕРНЕ»","authors":"S. V. Kosenko","doi":"10.33952/2542-0720-2021-2-26-109-115","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-109-115","url":null,"abstract":"Penza region is characterized by a diversity of natural and climatic zones and considerable variability of meteorological factors over the years. Therefore, the creation of high-yielding winter soft wheat varieties with high protein content in grain is one of the most significant challenges in this crop breeding. The purposes of this research are twofold: assess the ability of winter wheat varieties to form high-protein grain in various environmental conditions and isolate a valuable source material for breeding. The research objective is to evaluate winter wheat varieties by such indicator as “mass fraction of protein in grain”. The research was carried out from 2008 to 2017 in a forest-steppe zone of the Penza region. The soils of the experimental field – medium-humus medium-thick leached chernozem with the 35–40 cm depth of the plowing horizon. Ninety varieties of winter wheat from the world collection were the material for the research. The forecrop is black fallow. Square of experimental fields is 3 m2, triple replication. Seeding rate – 5.5 million germinating grains/ha. Standard – winter soft wheat variety ‘Fotinya’. Mass fraction of protein in grain in early ripening and mid-early varieties of winter wheat significantly differed from those of the mid-maturing group. Winter wheat varieties from the mid-ripening group were distinguished by a high protein content (on average 15.5 %), high yield (on average 3.8 t/ha), high yield of protein per hectare (on average 0.59 t/ha). According to the level of stress resistance, varieties ‘Fotinya’ and ‘Moskovskaya 39’ stood out (0.7 and 0.6, respectively). The following varieties are characterized by high genetic flexibility (15.3–16.2): ‘Fotinya’ (Penza region), ‘Moskovskaya 39’ (Moscow region), ‘Bezenchukskaya 380’ (Samara region), ‘Badulinka’ (Volgograd region), ‘Ershovskaya 25’ (Saratov region), ‘Zimorodok’, ‘Pobeda 50’ (Krasnodar Territory), ‘Zernogradka 8’, ‘Konkurent’ (Rostov Region). All the aforementioned varieties have less variability of a characteristic over the years (coefficient of variation – 3.2–9.7 %) and are recommended for use as a starting material for creating new forms.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87955358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-89-99
R. Zaremuk, T. A. Kopnina, Y. Dolya
There is an abnormal increase in average summer temperature in the southern region (Krasnodar Territory). This, more than ever, actualizes the problem of identifying drought-resistant varieties of fruit crops, including cherry. The purpose of this research was to study the physiological indicators of water regime: loss of water, water-holding capacity, leaf water content, abscisic acid content in leaves of different varieties of Cerasus vulgaris Mill., which determine resistance to temperature stress, as well as to identify the most drought-tolerant varieties to optimize the range of varieties for the south of our country. The studies were carried out in 2018–2019 in the Prikuban fruit-growing zone of the Krasnodar Territory. Sixteen varieties of Cerasus vulgaris Mill. were the objects of the research. Varieties ʻKrasnodarskaya sladkayaʼ and ʻKazachkaʼ served as a control. Planting schemes – 6 × 4 m and 5 × 3 m. Soil – chernozems leached. pHwater – 6.8–7.22. Humus content – 3.47 %. In summer, meteorological conditions were characterized by an abnormal temperature rise (up to 39.3 ℃) and extended periods of drought. Studying of water regime was carried out according to the modified methodology of M. D. Kushnerenko using the gravimetric method. In laboratory experiments, we used the capillary electrophoresis “Kapel 104 P” (power supply with a positive polarity; equipped with a photometric detector; set wavelength – 254 nm). In the course of the research, we revealed that the water content in cherry leaves during the period of the adverse draught (in July) was 57.9 %; in the context of studied cherry varieties, it varied insignificantly (Cv = 4.4 %). For two hours, the loss of water by the leaf apparatus was not high – from 6.5 % to 11.9 % with a low coefficient of variation by varieties (Cv = 1.9 %). The water-holding capacity of leaf tissues was 19.6 %, the range of variability of which was insignificant by varieties (Cv = 18.0–24.2 %). The average content of abscisic acid in cherry leaves was 4.3 mg/kg and varied depending on the studied cherry varieties within the range of 1.5–14.2 mg/kg. According to the complex of physiological indicators of the water regime (low water loss (6.5–7.9 %), high water-holding capacity (19.4–24.2 %) and leaf water content (58.1–61.9 %), as well as increased abscisic acids content (11.0–14.2 mg/kg)), such cherry varieties/cultivars as ʻDuke Ivanovnaʼ, ʻDuke Khodosaʼ, ʻFeyaʼ, ʻMolodezhnayaʼ and ʻErdi Botermoʼ were classified as highly drought-resistant. They are recommended for expanding the range of adaptive cherry varieties/cultivars in the southern part of Russia.
{"title":"Physiological aspects of drought resistance of cherry varieties","authors":"R. Zaremuk, T. A. Kopnina, Y. Dolya","doi":"10.33952/2542-0720-2021-2-26-89-99","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-89-99","url":null,"abstract":"There is an abnormal increase in average summer temperature in the southern region (Krasnodar Territory). This, more than ever, actualizes the problem of identifying drought-resistant varieties of fruit crops, including cherry. The purpose of this research was to study the physiological indicators of water regime: loss of water, water-holding capacity, leaf water content, abscisic acid content in leaves of different varieties of Cerasus vulgaris Mill., which determine resistance to temperature stress, as well as to identify the most drought-tolerant varieties to optimize the range of varieties for the south of our country. The studies were carried out in 2018–2019 in the Prikuban fruit-growing zone of the Krasnodar Territory. Sixteen varieties of Cerasus vulgaris Mill. were the objects of the research. Varieties ʻKrasnodarskaya sladkayaʼ and ʻKazachkaʼ served as a control. Planting schemes – 6 × 4 m and 5 × 3 m. Soil – chernozems leached. pHwater – 6.8–7.22. Humus content – 3.47 %. In summer, meteorological conditions were characterized by an abnormal temperature rise (up to 39.3 ℃) and extended periods of drought. Studying of water regime was carried out according to the modified methodology of M. D. Kushnerenko using the gravimetric method. In laboratory experiments, we used the capillary electrophoresis “Kapel 104 P” (power supply with a positive polarity; equipped with a photometric detector; set wavelength – 254 nm). In the course of the research, we revealed that the water content in cherry leaves during the period of the adverse draught (in July) was 57.9 %; in the context of studied cherry varieties, it varied insignificantly (Cv = 4.4 %). For two hours, the loss of water by the leaf apparatus was not high – from 6.5 % to 11.9 % with a low coefficient of variation by varieties (Cv = 1.9 %). The water-holding capacity of leaf tissues was 19.6 %, the range of variability of which was insignificant by varieties (Cv = 18.0–24.2 %). The average content of abscisic acid in cherry leaves was 4.3 mg/kg and varied depending on the studied cherry varieties within the range of 1.5–14.2 mg/kg. According to the complex of physiological indicators of the water regime (low water loss (6.5–7.9 %), high water-holding capacity (19.4–24.2 %) and leaf water content (58.1–61.9 %), as well as increased abscisic acids content (11.0–14.2 mg/kg)), such cherry varieties/cultivars as ʻDuke Ivanovnaʼ, ʻDuke Khodosaʼ, ʻFeyaʼ, ʻMolodezhnayaʼ and ʻErdi Botermoʼ were classified as highly drought-resistant. They are recommended for expanding the range of adaptive cherry varieties/cultivars in the southern part of Russia.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85912135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-8-18
I. Agasyeva, E. Fedorenko, M. Nefedova, A. Nastasiy
Populations of mites from the Tetranychidae family are characterized as high-resistant to acaricides, which, in turn, leads to an increase in the pesticide load on the agrocenoses ecosystem. Carnivorous arthropods from the Phytoseiidae family can be used as an alternative pest control. Our research aimed at improving the methods of breeding, storage and use of predatory mites against Tetrahychus urticae Koch. In 2015–2018, work was being undertaken to define the optimum thickness of the substrate layer (2.0, 4.0 and 6.0 cm) for breeding the food object – Acaris farus Oud. We also assessed the effect of the fodder substrate composition (wheat bran – control, wheat bran + soybean meal – experimental variant) on the population density of Amblyseius andersoni Chant. In 2016–2019, experiments on the storage of Neoseiulus barkeri Hughes, Neoseiulus cucumeris Oud. and Amblyseius swirskii Athias-Henriot at 4 °C (control – no storage) were carried out. In 2018–2019, to control Tetrahychus urticae Koch. in the soybean field, a mixture of N. cucumeris and A. andersoni was used according to the method of introduction into natural foci of prey. The optimum thickness of the substrate layer was found to be 4 cm. In this case, it was possible to obtain 6,983 mites in 1 cm3 for 7 days. Soybean meal addition increased the number of A. andersoni by 22.3 % compared to control (263 ind. vs 204 ind. per cm3). 30–45 days – an effective storage period for N. barkeri, 30 days – for N. cucumeris, no more than 10 days – for A. swirskii. On average, in 2018–2019, N. cucumeris and A. andersoni mixture reduced the number of T. urticae. The number of spider mites in the control variant was 14.6, in the experimental one – 5.3 ind./leaf. No predatory activity was observed on eggs. To prevent the development of spider mites, it is necessary to use N. cucumeris and A. andersoni at least twice with an interval of 5–7 days.
{"title":"Modifications of Phytoseiidae mite breeding methods to suppress spider mite","authors":"I. Agasyeva, E. Fedorenko, M. Nefedova, A. Nastasiy","doi":"10.33952/2542-0720-2021-2-26-8-18","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-8-18","url":null,"abstract":"Populations of mites from the Tetranychidae family are characterized as high-resistant to acaricides, which, in turn, leads to an increase in the pesticide load on the agrocenoses ecosystem. Carnivorous arthropods from the Phytoseiidae family can be used as an alternative pest control. Our research aimed at improving the methods of breeding, storage and use of predatory mites against Tetrahychus urticae Koch. In 2015–2018, work was being undertaken to define the optimum thickness of the substrate layer (2.0, 4.0 and 6.0 cm) for breeding the food object – Acaris farus Oud. We also assessed the effect of the fodder substrate composition (wheat bran – control, wheat bran + soybean meal – experimental variant) on the population density of Amblyseius andersoni Chant. In 2016–2019, experiments on the storage of Neoseiulus barkeri Hughes, Neoseiulus cucumeris Oud. and Amblyseius swirskii Athias-Henriot at 4 °C (control – no storage) were carried out. In 2018–2019, to control Tetrahychus urticae Koch. in the soybean field, a mixture of N. cucumeris and A. andersoni was used according to the method of introduction into natural foci of prey. The optimum thickness of the substrate layer was found to be 4 cm. In this case, it was possible to obtain 6,983 mites in 1 cm3 for 7 days. Soybean meal addition increased the number of A. andersoni by 22.3 % compared to control (263 ind. vs 204 ind. per cm3). 30–45 days – an effective storage period for N. barkeri, 30 days – for N. cucumeris, no more than 10 days – for A. swirskii. On average, in 2018–2019, N. cucumeris and A. andersoni mixture reduced the number of T. urticae. The number of spider mites in the control variant was 14.6, in the experimental one – 5.3 ind./leaf. No predatory activity was observed on eggs. To prevent the development of spider mites, it is necessary to use N. cucumeris and A. andersoni at least twice with an interval of 5–7 days.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91500756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-19-27
Mikhail V. Arkhipov
Traditional methods for evaluating sowing and technological characteristics of grain do not sufficiently reveal the caryopsis internal structure damages. They can only be recorded using the microfocus soft X-ray radiography method. The aim of this work was, with the help of X-ray, to evaluate the hidden integrity of the caryopsis structure (including the period of the post-harvest maturation) to increase the efficiency of expert assessment of the degree of economic suitability of grain batches. Seeds of Hordеum vulgare L. variety ‘Suzdalets’ harvested in 2015 in the Gatchina and Vsevolozhsk districts of the Leningrad region, malting barley from the Morskoy Port St. Petersburg (2015–2016), as well as corn grain (Zea mais L.) harvested in 2010 and 2011 were the objects of the research. Grain, which is for feed use only, was provided by the poultry farm “Severnaya”. The poultry farm “Roskar” provided samples harvested in 2017. We used the microfocus radiography method combined with a visual analysis of digital X-ray images to detect hidden grain defects. Experimental data on the evaluation of quality and biosafety of barley and maize grain for various purposes determined by radiographic signs of hidden damage are presented. The survey highlighted several key points: the seed lots obtained in the Vsevolozhsk district had a low total level of hidden defects (39 %); in the seed lots obtained in the Gatchina district, this figure was significantly higher (> 140 %). The germination rate for these options, determined six months after harvesting, turned out to be 89 and 74 %, respectively; it was higher for batches of seeds with a small value of the total indicator of hidden defects. Samples, the total level of hidden defects of which was lower (29 %), had a higher germination index (97 %) compared to the same in seed samples with a total level of hidden defects 70 % or more (85–90 %). X-ray analysis of corn grain samples obtained at the poultry farm “Roskar” revealed that the amount of grain with defects in the embryo (hidden germination) compared with the same in grain lots from the poultry farm “Severnaya” was low and varied from 17 to 29 %. The index of the hidden fissuring rate in these samples was not reliably recorded. However, we detected such internal grain damage as enzyme mycosis depletion (64 to 98 %). Research results are the basis for the development of new engineering and technical solutions for the “gentle” agricultural technologies to obtain batches of high-quality non-injured grain that ensure high competitiveness in the global grain market.
传统的评价籽粒播种和工艺特性的方法不能充分揭示颖果内部结构的损伤。它们只能用微聚焦软x射线照相法记录。本工作的目的是借助x射线对颖果结构的隐藏完整性(包括收获后成熟期)进行评估，以提高专家对粮食批次经济适宜度的评估效率。研究对象为2015年在列宁格勒地区Gatchina和Vsevolozhsk地区收获的hordapolum vulgare L.品种“Suzdalets”的种子、2015 - 2016年在圣彼得堡莫斯科伊港(Morskoy Port St. Petersburg)收获的麦芽以及2010年和2011年收获的玉米(Zea mais L.)。仅供饲料使用的谷物由“Severnaya”家禽养殖场提供。“罗斯卡尔”家禽养殖场提供了2017年收获的样本。我们使用微聚焦射线照相方法结合数字x射线图像的视觉分析来检测隐藏的颗粒缺陷。本文介绍了用射线示踪法测定各种用途大麦和玉米籽粒质量和生物安全评价的实验数据。调查突出了几个关键点:在Vsevolozhsk地区获得的种子地块的隐藏缺陷总水平较低(39%);在Gatchina地区获得的种子地块中，这一数字显著更高(> 140%)。这些选择的发芽率，在收获6个月后测定，分别为89%和74%;隐藏缺陷总指标值越小，批次的种子质量越高。隐藏缺陷总水平较低(29%)的种子萌发指数(97%)高于隐藏缺陷总水平在70%及以上(85 - 90%)的种子样品。对“Roskar”家禽养殖场获得的玉米颗粒样品进行x射线分析显示，与“Severnaya”家禽养殖场的谷物批次相比，胚胎缺陷(隐藏发芽)的谷物数量较低，从17%到29%不等。这些样品的隐裂率指标记录不可靠。然而，我们发现了诸如酵素真菌病耗损(64 - 98%)等谷物内部损害。研究成果是为“温和”农业技术开发新的工程技术解决方案的基础，以获得成批高质量的无损伤粮食，确保在全球粮食市场上具有较高的竞争力。
{"title":"Improving efficiency of operational control in the expert evaluation of grain quality","authors":"Mikhail V. Arkhipov","doi":"10.33952/2542-0720-2021-2-26-19-27","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-19-27","url":null,"abstract":"Traditional methods for evaluating sowing and technological characteristics of grain do not sufficiently reveal the caryopsis internal structure damages. They can only be recorded using the microfocus soft X-ray radiography method. The aim of this work was, with the help of X-ray, to evaluate the hidden integrity of the caryopsis structure (including the period of the post-harvest maturation) to increase the efficiency of expert assessment of the degree of economic suitability of grain batches. Seeds of Hordеum vulgare L. variety ‘Suzdalets’ harvested in 2015 in the Gatchina and Vsevolozhsk districts of the Leningrad region, malting barley from the Morskoy Port St. Petersburg (2015–2016), as well as corn grain (Zea mais L.) harvested in 2010 and 2011 were the objects of the research. Grain, which is for feed use only, was provided by the poultry farm “Severnaya”. The poultry farm “Roskar” provided samples harvested in 2017. We used the microfocus radiography method combined with a visual analysis of digital X-ray images to detect hidden grain defects. Experimental data on the evaluation of quality and biosafety of barley and maize grain for various purposes determined by radiographic signs of hidden damage are presented. The survey highlighted several key points: the seed lots obtained in the Vsevolozhsk district had a low total level of hidden defects (39 %); in the seed lots obtained in the Gatchina district, this figure was significantly higher (> 140 %). The germination rate for these options, determined six months after harvesting, turned out to be 89 and 74 %, respectively; it was higher for batches of seeds with a small value of the total indicator of hidden defects. Samples, the total level of hidden defects of which was lower (29 %), had a higher germination index (97 %) compared to the same in seed samples with a total level of hidden defects 70 % or more (85–90 %). X-ray analysis of corn grain samples obtained at the poultry farm “Roskar” revealed that the amount of grain with defects in the embryo (hidden germination) compared with the same in grain lots from the poultry farm “Severnaya” was low and varied from 17 to 29 %. The index of the hidden fissuring rate in these samples was not reliably recorded. However, we detected such internal grain damage as enzyme mycosis depletion (64 to 98 %). Research results are the basis for the development of new engineering and technical solutions for the “gentle” agricultural technologies to obtain batches of high-quality non-injured grain that ensure high competitiveness in the global grain market.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79462888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-116-126
E. Kostenkova, A. Bushnev
The issue of determining the ideotype of sunflower varieties and hybrids is relevant not only for breeding but also for cultivation in new environmental conditions. The aim of our research was to evaluate the genotypes according to ecological adaptability and establish the parameters of the sunflower ideotype for the arid conditions of the Crimean steppe zone. The studies were conducted in 2017-2020 at the Field Crop Department, FSBSI “Research Institute of Agriculture of Crimea” (village of Klepinino). Soil – chernozem southern low humus. Materials for the research – sunflower hybrids: ‘Garant’, ‘Komandor’, ‘Signal’, ‘Paritet’, ‘Prestizh’ (standard), ‘Sprint’, ‘Sprint 2’, ‘Gorstar’, ‘Kometa’ (bred in the All-Russian Scientific Research Institute of Oil Crops by the name of Pustovoit V.S.” (VNIIMK)). The experiment was replicated four times. The total area of the trial plot is 56 m2, the accounting area – 28 m2. Plant density – 40 thousand plants per ha. The harvest was brought to 100% purity; the seeds – to 10% moisture content. The linear regression coefficient (plasticity) of the yield of hybrids (bi) and the standard deviation (stability) (Ϭd2) were calculated according to S. A. Eberhart and W. A. Russell. Favourable weather conditions were in 2017 (Ij = +0.21) and 2019 (Ij = +1.04). Hybrids ‘Komandor’, ‘Garant’, ‘Sprint 2’ and ‘Gorstar’ are more responsive to the improvement of growing conditions (bi>1); variety ‘Kometa’ – weakly responsive (bi<1). When cultivation conditions changed, the yield of the hybrids ‘Signal’, ‘Paritet’, ‘Prestizh’ and ‘Sprint’ varied (bi = 1). In terms of yield, the most stable is ‘Kometa’ (Ϭd2 = 0.48); the most unstable – ‘Komandor’ (Ϭd2 = 2.19). According to the long-term field research, we have identified the parameters of the optimal model of a sunflower hybrid for cultivation in the Crimea: growing season length – 92–98 days, plant height – 161–166 cm, 1000-seeds weight – 69.5–83.0 g, productive area of the capitula (flower head) – 313–379 cm2, yield – 2.26–2.49 t/ha, oil content – 45–47%, as well as god responsiveness to the growing conditions improvement.
{"title":"Helianthus annuus L. hybrid ideotype","authors":"E. Kostenkova, A. Bushnev","doi":"10.33952/2542-0720-2021-2-26-116-126","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-116-126","url":null,"abstract":"The issue of determining the ideotype of sunflower varieties and hybrids is relevant not only for breeding but also for cultivation in new environmental conditions. The aim of our research was to evaluate the genotypes according to ecological adaptability and establish the parameters of the sunflower ideotype for the arid conditions of the Crimean steppe zone. The studies were conducted in 2017-2020 at the Field Crop Department, FSBSI “Research Institute of Agriculture of Crimea” (village of Klepinino). Soil – chernozem southern low humus. Materials for the research – sunflower hybrids: ‘Garant’, ‘Komandor’, ‘Signal’, ‘Paritet’, ‘Prestizh’ (standard), ‘Sprint’, ‘Sprint 2’, ‘Gorstar’, ‘Kometa’ (bred in the All-Russian Scientific Research Institute of Oil Crops by the name of Pustovoit V.S.” (VNIIMK)). The experiment was replicated four times. The total area of the trial plot is 56 m2, the accounting area – 28 m2. Plant density – 40 thousand plants per ha. The harvest was brought to 100% purity; the seeds – to 10% moisture content. The linear regression coefficient (plasticity) of the yield of hybrids (bi) and the standard deviation (stability) (Ϭd2) were calculated according to S. A. Eberhart and W. A. Russell. Favourable weather conditions were in 2017 (Ij = +0.21) and 2019 (Ij = +1.04). Hybrids ‘Komandor’, ‘Garant’, ‘Sprint 2’ and ‘Gorstar’ are more responsive to the improvement of growing conditions (bi>1); variety ‘Kometa’ – weakly responsive (bi<1). When cultivation conditions changed, the yield of the hybrids ‘Signal’, ‘Paritet’, ‘Prestizh’ and ‘Sprint’ varied (bi = 1). In terms of yield, the most stable is ‘Kometa’ (Ϭd2 = 0.48); the most unstable – ‘Komandor’ (Ϭd2 = 2.19). According to the long-term field research, we have identified the parameters of the optimal model of a sunflower hybrid for cultivation in the Crimea: growing season length – 92–98 days, plant height – 161–166 cm, 1000-seeds weight – 69.5–83.0 g, productive area of the capitula (flower head) – 313–379 cm2, yield – 2.26–2.49 t/ha, oil content – 45–47%, as well as god responsiveness to the growing conditions improvement.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78474100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-223-232
V. Sychev, Y. Grechishkina, V. Egorov, A. Matvienko
Irrigation is an important element of agricultural production in the arid conditions of the Stavropol Territory. The combination of irrigation, chemicalization and complex mechanization provides highly productive use of land that requires specific scientific support. The purpose of the research is to identify differences in the dynamics of the main agrochemical indicators (organic matter, pH, mobile phosphorus, mobile potassium) on irrigated and non-irrigated chestnut soils. The object of research is chestnut soils located in an extremely arid climatic zone of the Stavropol Territory. The data of complex monitoring of soil fertility in the Apanasenkovsky district were used as the source material. The data for the period from 1991 to 2020 were prepared by the staff of the State Center for Agrochemical Service “Stavropolsky”. The content of soil organic matter was determined by the Tyurin method in the modification of CINAO. Mobile compounds of phosphorus and potassium were determined by the Machigin method in the modification of CINAO. The value of the hydrogen pH index of the aqueous suspension was determined by the potentiometric method. The content of organic matter in chestnut soils increased from 1.8 to 2.0–2.1% both on irrigated and non-irrigated lands. A tendency to leaching of irrigated lands by 0.2 units has been revealed; therefore, in this case, it is recommended to use chemical ameliorants. Moreover, on irrigation, an increase in the content of mobile phosphorus (from 0.8 to 4.0 mg/kg) compared to non-irrigated areas was revealed. The reason for this is the increased doses of phosphorus-containing fertilizers applied during irrigation. During the study period, the content of mobile potassium increased from 77 to 93 mg/kg irrigated chestnut soils compared to non-irrigated ones. The negative dynamics in the content of mobile potassium on irrigation was revealed. It amounted to 364 mg/kg (as of 2020), which is lower than the data of the previous survey period by 81 mk/kg. Particular attention should be paid to the use of potash fertilizers since there is increased removal of this element, despite the high potash potential of chestnut soils in the arid zone of the Stavropol Territory.
{"title":"Assessment of the dynamics of the main agrochemical indicators of irrigated chestnut soils under risk farming conditions in Stavropol","authors":"V. Sychev, Y. Grechishkina, V. Egorov, A. Matvienko","doi":"10.33952/2542-0720-2021-2-26-223-232","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-223-232","url":null,"abstract":"Irrigation is an important element of agricultural production in the arid conditions of the Stavropol Territory. The combination of irrigation, chemicalization and complex mechanization provides highly productive use of land that requires specific scientific support. The purpose of the research is to identify differences in the dynamics of the main agrochemical indicators (organic matter, pH, mobile phosphorus, mobile potassium) on irrigated and non-irrigated chestnut soils. The object of research is chestnut soils located in an extremely arid climatic zone of the Stavropol Territory. The data of complex monitoring of soil fertility in the Apanasenkovsky district were used as the source material. The data for the period from 1991 to 2020 were prepared by the staff of the State Center for Agrochemical Service “Stavropolsky”. The content of soil organic matter was determined by the Tyurin method in the modification of CINAO. Mobile compounds of phosphorus and potassium were determined by the Machigin method in the modification of CINAO. The value of the hydrogen pH index of the aqueous suspension was determined by the potentiometric method. The content of organic matter in chestnut soils increased from 1.8 to 2.0–2.1% both on irrigated and non-irrigated lands. A tendency to leaching of irrigated lands by 0.2 units has been revealed; therefore, in this case, it is recommended to use chemical ameliorants. Moreover, on irrigation, an increase in the content of mobile phosphorus (from 0.8 to 4.0 mg/kg) compared to non-irrigated areas was revealed. The reason for this is the increased doses of phosphorus-containing fertilizers applied during irrigation. During the study period, the content of mobile potassium increased from 77 to 93 mg/kg irrigated chestnut soils compared to non-irrigated ones. The negative dynamics in the content of mobile potassium on irrigation was revealed. It amounted to 364 mg/kg (as of 2020), which is lower than the data of the previous survey period by 81 mk/kg. Particular attention should be paid to the use of potash fertilizers since there is increased removal of this element, despite the high potash potential of chestnut soils in the arid zone of the Stavropol Territory.","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89346703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.33952/2542-0720-2021-2-26-178-190
A. K. Svechnikov, L. Kozlova
Various ecological and biological factors, including the composition of grass mixtures in crop rotation, affect the state of agrophytocenosis. The purpose of the research was to reveal the regularities of changes in the botanical composition of crops in forage agrophytocenoses depending on the duration of perennial legume-cereal grasses cultivation. The experiment was conducted according to B. A. Dospekhov’s “Methods of field research” on the experimental field of the Mari Research Institute of Agriculture, a branch of the FSBSI “Federal Agricultural Research Center of the North-East” in 2013–2018. In a two-factor field experiment, we studied the botanical composition dynamics in three six-field grain-grass crop rotations and goat-rump grass mixture (Factor A) with and without nitrogen fertilizing against the background of phosphorus-potassium fertilizers (Factor B). Soil of the experimental plots – sod-podzolic mid loamy. The meteorological conditions of the growing seasons were often unfavorable; therefore, the crops were strongly suppressed. The most drought-resistant species (sunflower, winter rye, alfalfa and barley with a mass fraction of 55 %, 97 %, 20 % and 96 %, respectively) had an advantage over weeds in grass mixtures by weight in the spring-summer period during the third rotation of the studied crop rotations. Plants of red clover and white mustard in the catch crop were strongly suppressed by drought. The clover-alfalfa-timothy grass mixture was replaced by forbs from 36 % in mass in the first year of use with a step of 23 % in subsequent years. After the 15-year usage, the second component in the grass mixture monocrop (Galega orientalis Lam. + Bromus inermis Leyss) completely dropped out. Non-sown grasses faster replaced the crop components of the herbage on the fields without nitrogen fertilizing. Thus, the crop rotation fields with the richly diverse crops composition and the nitrogen application were infested with fewer weeds (4.0–10.6 % by mass).
各种生态和生物因素，包括轮作中混交草的组成，都会影响农植病的状态。本研究旨在揭示多年生豆科杂粮禾本科作物植物组成随栽培年限的变化规律。2013-2018年，在FSBSI“东北联邦农业研究中心”分支机构Mari农业研究所的实验田，根据B. a . Dospekhov的“实地研究方法”进行了实验。通过双因素田间试验，研究了在磷钾肥(因子B)背景下，三种六田粮草轮作和羊臀草混合(因子a)在施氮和不施氮条件下的植物组成动态。生长季节的气象条件往往不利;因此，农作物受到了强烈的抑制。在第三轮作物轮作期间，最抗旱的品种(向日葵、冬黑麦、苜蓿和大麦，其质量分数分别为55%、97%、20%和96%)在重量上优于混合草中的杂草。红三叶草和白芥菜的生长受到干旱的强烈抑制。在使用的第一年，三叶草-紫花苜蓿-蒂莫西草混合物被牧草取代，其质量为36%，随后几年为23%。经过15年的使用，混合草单作中第二组分含量显著高于对照。+褐雀花(Bromus inermis Leyss)完全退出。在不施氮肥的田地里，未播种的牧草更快地取代了牧草的作物成分。因此，施用氮肥、作物组成丰富的轮作田杂草数量较少(质量为4.0% ~ 10.6%)。
{"title":"Botanical composition dynamics in fodder agrophytocenoses based on legume-cereal herbs","authors":"A. K. Svechnikov, L. Kozlova","doi":"10.33952/2542-0720-2021-2-26-178-190","DOIUrl":"https://doi.org/10.33952/2542-0720-2021-2-26-178-190","url":null,"abstract":"Various ecological and biological factors, including the composition of grass mixtures in crop rotation, affect the state of agrophytocenosis. The purpose of the research was to reveal the regularities of changes in the botanical composition of crops in forage agrophytocenoses depending on the duration of perennial legume-cereal grasses cultivation. The experiment was conducted according to B. A. Dospekhov’s “Methods of field research” on the experimental field of the Mari Research Institute of Agriculture, a branch of the FSBSI “Federal Agricultural Research Center of the North-East” in 2013–2018. In a two-factor field experiment, we studied the botanical composition dynamics in three six-field grain-grass crop rotations and goat-rump grass mixture (Factor A) with and without nitrogen fertilizing against the background of phosphorus-potassium fertilizers (Factor B). Soil of the experimental plots – sod-podzolic mid loamy. The meteorological conditions of the growing seasons were often unfavorable; therefore, the crops were strongly suppressed. The most drought-resistant species (sunflower, winter rye, alfalfa and barley with a mass fraction of 55 %, 97 %, 20 % and 96 %, respectively) had an advantage over weeds in grass mixtures by weight in the spring-summer period during the third rotation of the studied crop rotations. Plants of red clover and white mustard in the catch crop were strongly suppressed by drought. The clover-alfalfa-timothy grass mixture was replaced by forbs from 36 % in mass in the first year of use with a step of 23 % in subsequent years. After the 15-year usage, the second component in the grass mixture monocrop (Galega orientalis Lam. + Bromus inermis Leyss) completely dropped out. Non-sown grasses faster replaced the crop components of the herbage on the fields without nitrogen fertilizing. Thus, the crop rotation fields with the richly diverse crops composition and the nitrogen application were infested with fewer weeds (4.0–10.6 % by mass).","PeriodicalId":22344,"journal":{"name":"TAURIDA HERALD OF THE AGRARIAN SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81131639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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