Pub Date : 2020-11-07DOI: 10.21608/ejh.2020.44091.1362
A. Shalaby, S. Kashef
Background:5-Fluorouracil(5-FU)is a common anti-tumor drug that is used in the management of different forms of malignancies. Mucosal inflammation of the oral cavity is the most common adverse effect of its administration. Melatonin has valuable activities like antioxidant and anti-inflammatory effects. Melatonin has protective effects on different organs against various side effects caused by anti-cancer treatments.Aim: To evaluate the protective effect of melatonin against 5-fluorouracil –induced changes in the tongue mucosa of rats using different histological techniques.Materials and Methods: Forty adult male rats were randomly divided into four main groups: group I acted as control, group II was given melatonin at a dose 10 mg/kg/day, group III was given 5-FU at a dose 60 mg/kg on day 0 and 40 mg/kg on day 2, and group IV was given melatonin one hour before 5-FU administration. The specimens of the tongue were processed for light and scanning electron microscopic study. Immunohistochemical study was done by employing the nuclear marker for proliferation of the cells(Ki67)antibody.Results: 5-FU induced structural changes in the tongue mucosa in the form of focal loss of lingual papillae, marked thinning and shortening of the filiform papillae, separation of the keratin layer from the underlying epithelium, vacuolated cytoplasm of the basal and suprabasal epithelial cells, and congestion of blood vessels with cellular infilteration in the lamina propria. A significant decrease in the papillae height, papillae width, ventral epithelial thickness, and percentage of Ki67 immunopositive cells were also detected. Scanning electron microscopy exhibited atrophy of the filiform papillae with desquamation of their epithelial covering. Deformed fungiform papillae with ill-defined taste pores were also revealed. On the other hand, these changes were less pronounced in rats received melatonin before 5-FU administration. Conclusion: 5-FU induced significant structural changes in the tongue mucosa of albino rats. Melatonin attenuated these mucosal changes.
{"title":"The Effect of 5-Fluorouracil on the tongue mucosa of adult male albino rat and the possible protective role of melatonin: A light and scanning electron microscopic study","authors":"A. Shalaby, S. Kashef","doi":"10.21608/ejh.2020.44091.1362","DOIUrl":"https://doi.org/10.21608/ejh.2020.44091.1362","url":null,"abstract":"Background:5-Fluorouracil(5-FU)is a common anti-tumor drug that is used in the management of different forms of malignancies. Mucosal inflammation of the oral cavity is the most common adverse effect of its administration. Melatonin has valuable activities like antioxidant and anti-inflammatory effects. Melatonin has protective effects on different organs against various side effects caused by anti-cancer treatments.Aim: To evaluate the protective effect of melatonin against 5-fluorouracil –induced changes in the tongue mucosa of rats using different histological techniques.Materials and Methods: Forty adult male rats were randomly divided into four main groups: group I acted as control, group II was given melatonin at a dose 10 mg/kg/day, group III was given 5-FU at a dose 60 mg/kg on day 0 and 40 mg/kg on day 2, and group IV was given melatonin one hour before 5-FU administration. The specimens of the tongue were processed for light and scanning electron microscopic study. Immunohistochemical study was done by employing the nuclear marker for proliferation of the cells(Ki67)antibody.Results: 5-FU induced structural changes in the tongue mucosa in the form of focal loss of lingual papillae, marked thinning and shortening of the filiform papillae, separation of the keratin layer from the underlying epithelium, vacuolated cytoplasm of the basal and suprabasal epithelial cells, and congestion of blood vessels with cellular infilteration in the lamina propria. A significant decrease in the papillae height, papillae width, ventral epithelial thickness, and percentage of Ki67 immunopositive cells were also detected. Scanning electron microscopy exhibited atrophy of the filiform papillae with desquamation of their epithelial covering. Deformed fungiform papillae with ill-defined taste pores were also revealed. On the other hand, these changes were less pronounced in rats received melatonin before 5-FU administration. Conclusion: 5-FU induced significant structural changes in the tongue mucosa of albino rats. Melatonin attenuated these mucosal changes.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"20 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85058660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-05DOI: 10.21608/ejh.2020.45532.1369
M. Sadek, S. S. Elabd, M. Ibrahim
Background: Chronic stress, evolving due to sudden unexpected life events, is a risk factor for the development of depression. It can suppress immunity and increase susceptibility to inflammatory diseases. Licorice extract is commonly used in traditional and modern medicine particularly for its antioxidant and anti-inflammatory properties. Aim of the work: This work aimed to perform a biochemical, histological, and immunohistochemical assessment of the influence of chronic unpredictable mild stress (CUMS) on the structure of the adrenal cortex of rat and the possible ameliorating role of licorice aqueous extract. Material and Methods: Twenty-four adult male albino rats were divided into 4 groups; control, licorice, CUMS (exposed to variable stressors in a random unpredictable pattern for 4 weeks), and licorice & CUMS (same as group III along with 300 mg/kg/day of licorice extract). Plasma aldosterone and corticosterone were assayed. Adrenal cortex specimens were processed for biochemical, histological, and immunohistochemical studies. Results: CUMS group recorded a significant elevation of plasma aldosterone, corticosterone, tissue malondialdehyde and myeloperoxidase associating with a significant reduction of tissue reduced glutathione and superoxide dismutase. Histological examination revealed adrenal capsular thickening and subcapsular hyperplasia, disruption of the architecture and thickness of all zones, and cellular alterations as nuclear abnormalities and cytoplasmic vacuolation. A significant upregulation in both activated caspase-3 and PCNA immunohistochemical expression was recorded. Licorice co-treated group depicted near normal values of most parameters with a near control morphology. Conclusion: Licorice could prove beneficial in controlling the adverse effects exerted by CUMS on redox status, morphology, apoptosis, and proliferation of the adrenal cortex most probably through its antioxidant, anti-inflammatory, and antiproliferative properties.
{"title":"Effect of Chronic Unpredictable Mild Stress on Adrenal Cortex of Adult Rat and The Possible Protective Role of Licorice Extract: A Histological and Immunohistochemical study","authors":"M. Sadek, S. S. Elabd, M. Ibrahim","doi":"10.21608/ejh.2020.45532.1369","DOIUrl":"https://doi.org/10.21608/ejh.2020.45532.1369","url":null,"abstract":"Background: Chronic stress, evolving due to sudden unexpected life events, is a risk factor for the development of depression. It can suppress immunity and increase susceptibility to inflammatory diseases. Licorice extract is commonly used in traditional and modern medicine particularly for its antioxidant and anti-inflammatory properties. Aim of the work: This work aimed to perform a biochemical, histological, and immunohistochemical assessment of the influence of chronic unpredictable mild stress (CUMS) on the structure of the adrenal cortex of rat and the possible ameliorating role of licorice aqueous extract. Material and Methods: Twenty-four adult male albino rats were divided into 4 groups; control, licorice, CUMS (exposed to variable stressors in a random unpredictable pattern for 4 weeks), and licorice & CUMS (same as group III along with 300 mg/kg/day of licorice extract). Plasma aldosterone and corticosterone were assayed. Adrenal cortex specimens were processed for biochemical, histological, and immunohistochemical studies. Results: CUMS group recorded a significant elevation of plasma aldosterone, corticosterone, tissue malondialdehyde and myeloperoxidase associating with a significant reduction of tissue reduced glutathione and superoxide dismutase. Histological examination revealed adrenal capsular thickening and subcapsular hyperplasia, disruption of the architecture and thickness of all zones, and cellular alterations as nuclear abnormalities and cytoplasmic vacuolation. A significant upregulation in both activated caspase-3 and PCNA immunohistochemical expression was recorded. Licorice co-treated group depicted near normal values of most parameters with a near control morphology. Conclusion: Licorice could prove beneficial in controlling the adverse effects exerted by CUMS on redox status, morphology, apoptosis, and proliferation of the adrenal cortex most probably through its antioxidant, anti-inflammatory, and antiproliferative properties.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"9 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89768450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-04DOI: 10.21608/ejh.2020.44047.1361
N. Issa, D. Noya, R. Salama
Background: Hyperlipidemia is a common problem. It is a well-known aggravating factor in the pathogenesis of many diseases so that, this study was planned to study the structural changes of parotid gland in Triton WR-1339-induced hyperlipidemia rat model and to investigate the (protecting and treating) effect of Thymus vulgaris extract (Thyme) as a natural product. Materials and methods: Fifty adult male albino rats were randomly divided into five groups. Group I (Control G), Group II (Thyme G): rats received Thyme (500 mg/kg body weight) for ten days, Group III (Hyperlipidemia G): rats received a single intraperitoneal dose of 1.0 ml Triton WR1339. Group IV (Protected G): rats received Thyme for ten days and the induction of hyperlipidemia occurred on the seventh day of the experiment. Group V (Treated G): rats received Thyme 24 hours after induction and throughout the experiment. Three days after induction of hyperlipidemia, blood samples were collected to measure serum total cholesterol & triglycerides. The parotid gland was processed for histological, histochemical and immmunohistochemical studies. Morphometrical and statistical analysis were done. Results: The hyperlipidemia rat model induced by Triton WR1339 developed acute inflammation of parotid gland leading to obvious histological and histochemical findings and significant increase in the immunohistochemical markers of inflammation and fibrosis. These findings were ameliorated by thyme as a protecting agent and to a significant lesser extent as a treatment. Concolusion: Thyme can be used as a protective agent against the parotid gland lesions caused by hyperlipidemia through its anti-inflammatory, antioxidant and hypolipidemic properties.
{"title":"Evaluation of Thymus vulgaris (Thyme) role in the protection and treatment of the parotid gland of Triton WR-1339 induced hyperlipidemia in adult male albino rats","authors":"N. Issa, D. Noya, R. Salama","doi":"10.21608/ejh.2020.44047.1361","DOIUrl":"https://doi.org/10.21608/ejh.2020.44047.1361","url":null,"abstract":"Background: Hyperlipidemia is a common problem. It is a well-known aggravating factor in the pathogenesis of many diseases so that, this study was planned to study the structural changes of parotid gland in Triton WR-1339-induced hyperlipidemia rat model and to investigate the (protecting and treating) effect of Thymus vulgaris extract (Thyme) as a natural product. Materials and methods: Fifty adult male albino rats were randomly divided into five groups. Group I (Control G), Group II (Thyme G): rats received Thyme (500 mg/kg body weight) for ten days, Group III (Hyperlipidemia G): rats received a single intraperitoneal dose of 1.0 ml Triton WR1339. Group IV (Protected G): rats received Thyme for ten days and the induction of hyperlipidemia occurred on the seventh day of the experiment. Group V (Treated G): rats received Thyme 24 hours after induction and throughout the experiment. Three days after induction of hyperlipidemia, blood samples were collected to measure serum total cholesterol & triglycerides. The parotid gland was processed for histological, histochemical and immmunohistochemical studies. Morphometrical and statistical analysis were done. Results: The hyperlipidemia rat model induced by Triton WR1339 developed acute inflammation of parotid gland leading to obvious histological and histochemical findings and significant increase in the immunohistochemical markers of inflammation and fibrosis. These findings were ameliorated by thyme as a protecting agent and to a significant lesser extent as a treatment. Concolusion: Thyme can be used as a protective agent against the parotid gland lesions caused by hyperlipidemia through its anti-inflammatory, antioxidant and hypolipidemic properties.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"47 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83226653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-30DOI: 10.21608/ejh.2020.44196.1363
Heba H. Elkaliny, S. S. Elabd, M. Ibrahim
Background: Immobilization stress occurs in various clinical situations as chronic inflammatory and neuromuscular diseases leading to disuse muscle atrophy. Recovery of skeletal muscle after immobilization is slow and incomplete. Platelet-rich plasma (PRP) was previously used in improving traumatic muscle injuries, yet, its role in accelerating muscle recovery upon immobilization stress has not been studied. Aim of the work: to study the possible adjuvant effect of PRP on skeletal muscle atrophy during recovery after immobilization stress in rat using different histological and immunohistochemical teschniques.Material and Methods: Thirty-six adult male rats were equally divided into four groups; control, immobilization stress (restrained in reduced sized cages for 4 weeks), recovery alone (4 weeks), and recovery & PRP groups. Skeletal muscle specimens were processed for histological and immunohistochemical studies.Results: Immobilized group showed splitting of the muscle fibers, internalization of the nuclei, loss of striation, undulated disrupted sarcolemma with signs of inflammation. Ultrastructural examination revealed myocytes with indented nuclei, mitochondria of abnormal shape and size, atrophied myofibrils with loss of myofilaments and splitting of myofibrils. A significant reduction of both mean muscle fiber cross sectional area and area percentage was reported together with a significant downregulation in desmin immunohistochemical expression. Recovery group revealed persistence of histological and immunohistochemical alterations. Recovery & PRP group showed a near normal morphology together with a non-significant difference in desmin immunohistochemical expression compared to control.Conclusion: PRP offered a promising improvement of skeletal muscle atrophy after immobilization stress. It is recommended to apply adjuvant PRP therapy with recovery rather than recovery alone.
{"title":"Platelet-rich plasma enhances recovery of skeletal muscle atrophy after immobilization stress in rat: A histological and immunohistochemical study","authors":"Heba H. Elkaliny, S. S. Elabd, M. Ibrahim","doi":"10.21608/ejh.2020.44196.1363","DOIUrl":"https://doi.org/10.21608/ejh.2020.44196.1363","url":null,"abstract":"Background: Immobilization stress occurs in various clinical situations as chronic inflammatory and neuromuscular diseases leading to disuse muscle atrophy. Recovery of skeletal muscle after immobilization is slow and incomplete. Platelet-rich plasma (PRP) was previously used in improving traumatic muscle injuries, yet, its role in accelerating muscle recovery upon immobilization stress has not been studied. Aim of the work: to study the possible adjuvant effect of PRP on skeletal muscle atrophy during recovery after immobilization stress in rat using different histological and immunohistochemical teschniques.Material and Methods: Thirty-six adult male rats were equally divided into four groups; control, immobilization stress (restrained in reduced sized cages for 4 weeks), recovery alone (4 weeks), and recovery & PRP groups. Skeletal muscle specimens were processed for histological and immunohistochemical studies.Results: Immobilized group showed splitting of the muscle fibers, internalization of the nuclei, loss of striation, undulated disrupted sarcolemma with signs of inflammation. Ultrastructural examination revealed myocytes with indented nuclei, mitochondria of abnormal shape and size, atrophied myofibrils with loss of myofilaments and splitting of myofibrils. A significant reduction of both mean muscle fiber cross sectional area and area percentage was reported together with a significant downregulation in desmin immunohistochemical expression. Recovery group revealed persistence of histological and immunohistochemical alterations. Recovery & PRP group showed a near normal morphology together with a non-significant difference in desmin immunohistochemical expression compared to control.Conclusion: PRP offered a promising improvement of skeletal muscle atrophy after immobilization stress. It is recommended to apply adjuvant PRP therapy with recovery rather than recovery alone.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"128 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88033356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-29DOI: 10.21608/ejh.2020.44558.1366
S. Ouies, S. Roshdy, Assma Baset
Background: There are different responses of lead exposure including reduced fertility, spontaneous abortions, low birth weight, impairment in folliculogenesis, and even damage to the ovaries are also reported. Several reports confirmed the usefulness of black seed oil because of having more than one hundred of components as volatile oil, vitamins and trace elements. Recently, clinical and animal studies revealed that the black seed extract has many therapeutic effects. Aim of work: To study the development of the ovary of the offspring of female rats exposed to lead acetate during pregnancy and lactation and the possible protective role of nigella sativa oil. Materials and methods: three groups were used; Group A: Includes 15 offspring of 20 control mothers, Group B: Includes 15 offspring of 20 mothers treated with orally lead acetate in a dose of 640mg/kg, Group c: includes 15 offspring of 20 mothers in addition to the same dose of lead acetate each rat was takenorally nigella sativa oil in a dose of 10mg/kg.Both treatments were given to mothers from gestational day 10 to post-natal day 21.After the last dose the abdomens were opened and the ovaries were removed and processed for light and transmission electron microscopic study.Results:Prenatal lead exposure caused Changes in ovarian architecture andsevere pathological changes in the ovarian follicles in the form of delayed development of primordial follicles, damage to the granulosa cells and degeneration to the oocyteswhich appeared shrunken with vacuolated cytoplasm and destructed zonapellucida. Administration of nigella sativa oil prevent the damaging effect of lead on the ovarian follicles, still some oocytes and follicles preserved its normal appearance. Conclusion: Exposure of mothers to lead acetate products cause harmful effects on the ovaries of the offspring, Administration of nigella sativa oil has an ameliorative effects on these damaging effects of lead acetate.
{"title":"Effect of Lead Acetate on the ovarian growth in the Pre-pubertal and Pubertal periods in the offspring of Albino Rats and the Possible Protective Role of Nigella Sativa oil","authors":"S. Ouies, S. Roshdy, Assma Baset","doi":"10.21608/ejh.2020.44558.1366","DOIUrl":"https://doi.org/10.21608/ejh.2020.44558.1366","url":null,"abstract":"Background: There are different responses of lead exposure including reduced fertility, spontaneous abortions, low birth weight, impairment in folliculogenesis, and even damage to the ovaries are also reported. Several reports confirmed the usefulness of black seed oil because of having more than one hundred of components as volatile oil, vitamins and trace elements. Recently, clinical and animal studies revealed that the black seed extract has many therapeutic effects. Aim of work: To study the development of the ovary of the offspring of female rats exposed to lead acetate during pregnancy and lactation and the possible protective role of nigella sativa oil. Materials and methods: three groups were used; Group A: Includes 15 offspring of 20 control mothers, Group B: Includes 15 offspring of 20 mothers treated with orally lead acetate in a dose of 640mg/kg, Group c: includes 15 offspring of 20 mothers in addition to the same dose of lead acetate each rat was takenorally nigella sativa oil in a dose of 10mg/kg.Both treatments were given to mothers from gestational day 10 to post-natal day 21.After the last dose the abdomens were opened and the ovaries were removed and processed for light and transmission electron microscopic study.Results:Prenatal lead exposure caused Changes in ovarian architecture andsevere pathological changes in the ovarian follicles in the form of delayed development of primordial follicles, damage to the granulosa cells and degeneration to the oocyteswhich appeared shrunken with vacuolated cytoplasm and destructed zonapellucida. Administration of nigella sativa oil prevent the damaging effect of lead on the ovarian follicles, still some oocytes and follicles preserved its normal appearance. Conclusion: Exposure of mothers to lead acetate products cause harmful effects on the ovaries of the offspring, Administration of nigella sativa oil has an ameliorative effects on these damaging effects of lead acetate.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"73 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85939126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-26DOI: 10.21608/ejh.2020.37873.1339
R. Salem, D. ELSahra, H. Osman, A. El-Kenawy
Recently many researchers reported that treatment using cyclosporine A in extended period in order to avoide the rejection of allograft is connected with renal impairment progress. Arabic gum is a valuable antioxidants and anti-carcinogenic factor with a protective effect against renal intoxications. This research aims at assessing the improving function of Arabic gum in ameliorating nephrotoxicity of cyclosporine A in adult rats by measuring urea and creatinine as renal function tests and histopathological changes plus redox status parameters. 80 mature rats males and females were classified into four groups during the period of expirment (45 days) first one act as control given saline; the second one was given 7.5gm/kg/day of Arabic gum ,while third group was given cyclosporine A( 25 mg/kg /day ) finally the fourth one was given 7.5gm/kg/day of Arabic gum together with the 25 mg/kg /day of cyclosporine A. Cyclosporine A group three rats had renal impairment beside injury, when reported by elevated values of urea and creatinine and renal pathological changes, relative to the first group(control) with high level of cyclosporine A in blood and bad parameters of redox status. Receiving of (Arabic gum) accompanied with (cyclosporine A) ameliorating renal damage by decreasing the level of urea and creatinine in the blood, renal histo pathological amelioration, moreover the improved parametersof antioxidant. The levels of catalase, glutathione, peroxidase and superoxide dismutase were statistically reduced as regard group three G3 (cyclosporine) which was significant relative to the control & second groups G1,G2 although significantly elevated in the fourth group which given cyclosporine plus Arabic gum when compared with the third group (cyclosporine). Arabic gum has likely to behave like natural agent opposing cyclosporine A -provoked nephrotoxicity.
{"title":"Arabic Gum Role In Ameliorating Cyclosporine- A Initiated Nephrotoxicity In Albino Rats","authors":"R. Salem, D. ELSahra, H. Osman, A. El-Kenawy","doi":"10.21608/ejh.2020.37873.1339","DOIUrl":"https://doi.org/10.21608/ejh.2020.37873.1339","url":null,"abstract":"Recently many researchers reported that treatment using cyclosporine A in extended period in order to avoide the rejection of allograft is connected with renal impairment progress. Arabic gum is a valuable antioxidants and anti-carcinogenic factor with a protective effect against renal intoxications. This research aims at assessing the improving function of Arabic gum in ameliorating nephrotoxicity of cyclosporine A in adult rats by measuring urea and creatinine as renal function tests and histopathological changes plus redox status parameters. 80 mature rats males and females were classified into four groups during the period of expirment (45 days) first one act as control given saline; the second one was given 7.5gm/kg/day of Arabic gum ,while third group was given cyclosporine A( 25 mg/kg /day ) finally the fourth one was given 7.5gm/kg/day of Arabic gum together with the 25 mg/kg /day of cyclosporine A. Cyclosporine A group three rats had renal impairment beside injury, when reported by elevated values of urea and creatinine and renal pathological changes, relative to the first group(control) with high level of cyclosporine A in blood and bad parameters of redox status. Receiving of (Arabic gum) accompanied with (cyclosporine A) ameliorating renal damage by decreasing the level of urea and creatinine in the blood, renal histo pathological amelioration, moreover the improved parametersof antioxidant. The levels of catalase, glutathione, peroxidase and superoxide dismutase were statistically reduced as regard group three G3 (cyclosporine) which was significant relative to the control & second groups G1,G2 although significantly elevated in the fourth group which given cyclosporine plus Arabic gum when compared with the third group (cyclosporine). Arabic gum has likely to behave like natural agent opposing cyclosporine A -provoked nephrotoxicity.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"358 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82625072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-24DOI: 10.21608/ejh.2020.45310.1368
Rabab Hassan, N. Kabel
Background: Tooth avulsion is one of traumatic injuries that cause damage to periodontal ligament (PDL) tissues. Avulsed tooth should be stored in a suitable storage medium to preserve the vitality of the PDL fibroblasts (PDLFs).Aim: To assess and compare the effect of various media on albino rabbit’s PDLFs viability and basic fibroblast growth factor (b-FGF) protein and gene expressions at different time intervals.Material and methods: periodontal ligament stem cells (PDLSCs) were isolated from molars of albino rabbit then differentiated into PDLFs. The PDLFs were cultured in different media (contact lens solution, skimmed milk, Dentosafe and alcohol free mouthwash) for 1h, 12h and 24h. Phosphate buffered saline (PBS) was used as control medium. Cell viability was assessed by MTT assay. b-FGF protein and gene expression levels were detected by immunofluorescence (IF) staining and Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) respectively. Data from MTT assay and H-score of IF staining were statistically analyzed using ANOVA test and multiple two groups comparisons pos hoc test.Results: examination of isolated PDLSCs at different passages showed various shapes of cells from rounded to spindle shaped. The differentiated PDLFs showed positive IF reaction. Statistical analysis of tested media at different time intervals represented that; PBS had the highest mean of fibroblastic cell viability% and H-score, followed by Dentosafe, skimmed milk, contact lens solution and mouthwash respectively. Gene expression of b-FGF was up-regulated in Dentosafe and skimmed milk while it was down-regulated in mouthwash.Conclusions: Dentosafe is more effective in maintenance of PDLFs viability and function than the other tested media. Mouthwash is not recommended as a storage media for PDLFs.
{"title":"Comparative Evaluation of Viability and Function of Albino Rabbit Periodontal Ligament Fibroblasts in Various Media (In Vitro Study)","authors":"Rabab Hassan, N. Kabel","doi":"10.21608/ejh.2020.45310.1368","DOIUrl":"https://doi.org/10.21608/ejh.2020.45310.1368","url":null,"abstract":"Background: Tooth avulsion is one of traumatic injuries that cause damage to periodontal ligament (PDL) tissues. Avulsed tooth should be stored in a suitable storage medium to preserve the vitality of the PDL fibroblasts (PDLFs).Aim: To assess and compare the effect of various media on albino rabbit’s PDLFs viability and basic fibroblast growth factor (b-FGF) protein and gene expressions at different time intervals.Material and methods: periodontal ligament stem cells (PDLSCs) were isolated from molars of albino rabbit then differentiated into PDLFs. The PDLFs were cultured in different media (contact lens solution, skimmed milk, Dentosafe and alcohol free mouthwash) for 1h, 12h and 24h. Phosphate buffered saline (PBS) was used as control medium. Cell viability was assessed by MTT assay. b-FGF protein and gene expression levels were detected by immunofluorescence (IF) staining and Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) respectively. Data from MTT assay and H-score of IF staining were statistically analyzed using ANOVA test and multiple two groups comparisons pos hoc test.Results: examination of isolated PDLSCs at different passages showed various shapes of cells from rounded to spindle shaped. The differentiated PDLFs showed positive IF reaction. Statistical analysis of tested media at different time intervals represented that; PBS had the highest mean of fibroblastic cell viability% and H-score, followed by Dentosafe, skimmed milk, contact lens solution and mouthwash respectively. Gene expression of b-FGF was up-regulated in Dentosafe and skimmed milk while it was down-regulated in mouthwash.Conclusions: Dentosafe is more effective in maintenance of PDLFs viability and function than the other tested media. Mouthwash is not recommended as a storage media for PDLFs.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"9 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82029053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-23DOI: 10.21608/ejh.2020.36819.1350
S. Ouies, Z. Ismail, Mostafa Atya
Background: Pregabalin is an oral medication that is used to treat neuropathic pain, generalized anxiety disorder and epilepsy; as a therapy for partial seizures. Cerebellar development is not complete during gestation only; but continues after birth to maturation of the cerebellum (postnatal development).Aim of the Work: To study the possible effect of Pregabalin on the postnatal development of the cerebellar cortex of albino rat.Materialandmethods:60 pregnant albino rats were allocated into three groups; Group A: Included 20offspring of 20 control mothers, Group B: Included 20 offspring of 20 mothers treated orally with pregabalin at a dose of 150mg/kg, and Group C:included 25 offspring of 20 mothers treated orally with pregabalin at a dose of 600mg/kg. In the three groups rats were sacrificed at postnatal (age 1, 2, 3 weeks), in addition to adult age (2.5 months).The skulls were opened and the cerebella were removed and processed for light and transmission electron microscope. Results: Pregabalin administration during pregnancy and lactation caused its marked effect during early postnatal life and this effect extended till the adult stage; in the form of increase in the external granular layer (mainly in the 2nd and 3rd weeks); less thickness of the molecular layer in all treated groups; shrunken and abnormal shaped Purkinje cells which appeared with ill-defined cell membranes and destructed nuclei; mainly at higher dose. There were also delayed differentiation of the internal granular layer and granule cells appeared with destructed cytoplasmic membrane and organelles which appeared prominent at higher doses. Conclusion: Pre and postnatal administration of pregabalin in both low and high doses caused loss of cellular components, distortions of cerebellar cortical cells in a dose dependent manner.
{"title":"The Possible Effect of pregabalin on the postnatal development of the cerebellar cortex in albino rats (Histological &Ultrastrucural study).","authors":"S. Ouies, Z. Ismail, Mostafa Atya","doi":"10.21608/ejh.2020.36819.1350","DOIUrl":"https://doi.org/10.21608/ejh.2020.36819.1350","url":null,"abstract":"Background: Pregabalin is an oral medication that is used to treat neuropathic pain, generalized anxiety disorder and epilepsy; as a therapy for partial seizures. Cerebellar development is not complete during gestation only; but continues after birth to maturation of the cerebellum (postnatal development).Aim of the Work: To study the possible effect of Pregabalin on the postnatal development of the cerebellar cortex of albino rat.Materialandmethods:60 pregnant albino rats were allocated into three groups; Group A: Included 20offspring of 20 control mothers, Group B: Included 20 offspring of 20 mothers treated orally with pregabalin at a dose of 150mg/kg, and Group C:included 25 offspring of 20 mothers treated orally with pregabalin at a dose of 600mg/kg. In the three groups rats were sacrificed at postnatal (age 1, 2, 3 weeks), in addition to adult age (2.5 months).The skulls were opened and the cerebella were removed and processed for light and transmission electron microscope. Results: Pregabalin administration during pregnancy and lactation caused its marked effect during early postnatal life and this effect extended till the adult stage; in the form of increase in the external granular layer (mainly in the 2nd and 3rd weeks); less thickness of the molecular layer in all treated groups; shrunken and abnormal shaped Purkinje cells which appeared with ill-defined cell membranes and destructed nuclei; mainly at higher dose. There were also delayed differentiation of the internal granular layer and granule cells appeared with destructed cytoplasmic membrane and organelles which appeared prominent at higher doses. Conclusion: Pre and postnatal administration of pregabalin in both low and high doses caused loss of cellular components, distortions of cerebellar cortical cells in a dose dependent manner.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"1 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81788069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-17DOI: 10.21608/ejh.2020.42776.1356
E. Mohamed, M. Hamid, Yara M Elfakharany
Introduction: Cadmium is a toxic heavy metal naturally present in plant food and unavoidable pollutant for many dental procedures. This metal shares in toxic peroxidation and destruction of macromolecules in different tissues, besides inhibition of DNA repair enzymes. The tongue is one of the vital organs that could be affected by chronic cadmium toxicity, especially the filiform papillae and tongue skeletal muscles due to their high metabolic activity. Aim of work: to study the possible role of Zinc oxide nanoparticles (ZnO NPs) in attenuation of the structural, ultra-structural and biochemical changes in rats' tongues induced by Cadmium chloride. Materials and Methods: sixty adult male Albino rats were divided equally -according to the utilized substance- into three groups; control, Cadmium chloride (CdCl) and (CdCl+ ZnO NPs) groups; every group received the allocated materials for 4 weeks. Samples were processed for light, electron microscopic examinations and biochemical analysis (malondialdehyde; reduced glutathione; catalase; superoxide dismutase; glutathione peroxidase). Results: Cadmium produced various apparent histopathological and biochemical alterations in tongue tissues in the form of filiform papillae with blunted ends, and thick keratin plaques. Epithelial cell layers appeared with vacuolated cytoplasm and dark nuclei. Degenerated muscle fibrils with wide spaces between them and many collagen fibers. Significant decrease of tongue reduced GSH, SOD, GPx and Cat enzymes activity and significant increase in MDA level in Cadmium chloride group as compared to control group. ZnO NPs attenuated these changes and revealed pointed tipped filiform papillae, nearly normal cells and Parallel arrangements of myofibrils. The levels of reduced GSH, SOD, GPx, Cat and MDA were insignificant compared to control group. Conclusion: Zinc oxide nanoparticles (ZnO NPs) attenuated the structural and biochemical changes in rats' tongues induced by Cadmium chloride.
{"title":"Zinc Oxide Nanoparticles attenuate Cadmium Chloride induced tongue toxicity in adult male albino rats (Histological and Biochemical study)","authors":"E. Mohamed, M. Hamid, Yara M Elfakharany","doi":"10.21608/ejh.2020.42776.1356","DOIUrl":"https://doi.org/10.21608/ejh.2020.42776.1356","url":null,"abstract":"Introduction: Cadmium is a toxic heavy metal naturally present in plant food and unavoidable pollutant for many dental procedures. This metal shares in toxic peroxidation and destruction of macromolecules in different tissues, besides inhibition of DNA repair enzymes. The tongue is one of the vital organs that could be affected by chronic cadmium toxicity, especially the filiform papillae and tongue skeletal muscles due to their high metabolic activity. Aim of work: to study the possible role of Zinc oxide nanoparticles (ZnO NPs) in attenuation of the structural, ultra-structural and biochemical changes in rats' tongues induced by Cadmium chloride. Materials and Methods: sixty adult male Albino rats were divided equally -according to the utilized substance- into three groups; control, Cadmium chloride (CdCl) and (CdCl+ ZnO NPs) groups; every group received the allocated materials for 4 weeks. Samples were processed for light, electron microscopic examinations and biochemical analysis (malondialdehyde; reduced glutathione; catalase; superoxide dismutase; glutathione peroxidase). Results: Cadmium produced various apparent histopathological and biochemical alterations in tongue tissues in the form of filiform papillae with blunted ends, and thick keratin plaques. Epithelial cell layers appeared with vacuolated cytoplasm and dark nuclei. Degenerated muscle fibrils with wide spaces between them and many collagen fibers. Significant decrease of tongue reduced GSH, SOD, GPx and Cat enzymes activity and significant increase in MDA level in Cadmium chloride group as compared to control group. ZnO NPs attenuated these changes and revealed pointed tipped filiform papillae, nearly normal cells and Parallel arrangements of myofibrils. The levels of reduced GSH, SOD, GPx, Cat and MDA were insignificant compared to control group. Conclusion: Zinc oxide nanoparticles (ZnO NPs) attenuated the structural and biochemical changes in rats' tongues induced by Cadmium chloride.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"24 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75873943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-15DOI: 10.21608/ejh.2020.38061.1342
M. Sabry, Rahma Abou Elnour, Marwaa M. Abd-El Monem, Gihan Ibrahim Aboul Fotouh
AbstractBackground and Objectives: Nicotine in cigarette smoking possesses damaging effect on testis. This study was designed to evaluate and compare the prophylactic and therapeutic effects of β carotene on nicotine induced testicular damage during nicotine withdrawal.Materials and Methods:Thirty-nine adult male albino rats were used, 9 rats were control (GI), the remaining rats divided equally into 5 groups: GII (β carotene), GIII (Nicotine), GIV(Nicotine withdrawal), GV (Nicotine withdrawal therapeutic), GVI (Nicotine withdrawal prophylactic group). β carotene (10mg/kg/day) was injected intraperitoneally for 4 weeks. Nicotine (4 mg/kg/day) was injected intraperitoneally for 4 weeks. Before sacrifaction, blood samples were drawn to assess serum levels of malondialdehyde (MDA) and testosterone. After sacrifaction, testicular sections were stained with H&E, Masson trichrome (to reveal collagen fiber deposition and distribution) and immunohistochemically for Vimentin (Sertoli cell intermediate filament(. Morphometric measurements were done by computerized image analysis.Results:Groups I and II showed similar results with normal testicular histological architecture, biochemical and morphometrical results. Deterioration of histological architecture, biochemical and morphometric parameters were recorded in groups III, IV and V (mostly GIII) with significant decrease in diameter of seminiferous tubules, height of spermatogenic epithelium, area percent of vimentin reaction and significant increase in area percent of collagen fibres deposition compared to GI. GVI showed better results than GIII, GIV and GV with restoration of normal testicular histological structure.Conclusion: β carotene could help in improving spermatogenesis. Administration of β carotene prophylactically during nicotine exposure and withdrawal gives better histological, biochemical and morphometrical results than therapeutic administration during nicotine withdrawal period only.
{"title":"The Possible Effect of β -carotene on Nicotine Withdrawal in Testicular Tissue of Adult Male Albino Rats. Histological and Immunohistochemical Study.","authors":"M. Sabry, Rahma Abou Elnour, Marwaa M. Abd-El Monem, Gihan Ibrahim Aboul Fotouh","doi":"10.21608/ejh.2020.38061.1342","DOIUrl":"https://doi.org/10.21608/ejh.2020.38061.1342","url":null,"abstract":"AbstractBackground and Objectives: Nicotine in cigarette smoking possesses damaging effect on testis. This study was designed to evaluate and compare the prophylactic and therapeutic effects of β carotene on nicotine induced testicular damage during nicotine withdrawal.Materials and Methods:Thirty-nine adult male albino rats were used, 9 rats were control (GI), the remaining rats divided equally into 5 groups: GII (β carotene), GIII (Nicotine), GIV(Nicotine withdrawal), GV (Nicotine withdrawal therapeutic), GVI (Nicotine withdrawal prophylactic group). β carotene (10mg/kg/day) was injected intraperitoneally for 4 weeks. Nicotine (4 mg/kg/day) was injected intraperitoneally for 4 weeks. Before sacrifaction, blood samples were drawn to assess serum levels of malondialdehyde (MDA) and testosterone. After sacrifaction, testicular sections were stained with H&E, Masson trichrome (to reveal collagen fiber deposition and distribution) and immunohistochemically for Vimentin (Sertoli cell intermediate filament(. Morphometric measurements were done by computerized image analysis.Results:Groups I and II showed similar results with normal testicular histological architecture, biochemical and morphometrical results. Deterioration of histological architecture, biochemical and morphometric parameters were recorded in groups III, IV and V (mostly GIII) with significant decrease in diameter of seminiferous tubules, height of spermatogenic epithelium, area percent of vimentin reaction and significant increase in area percent of collagen fibres deposition compared to GI. GVI showed better results than GIII, GIV and GV with restoration of normal testicular histological structure.Conclusion: β carotene could help in improving spermatogenesis. Administration of β carotene prophylactically during nicotine exposure and withdrawal gives better histological, biochemical and morphometrical results than therapeutic administration during nicotine withdrawal period only.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"29 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76021218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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