Pub Date : 2020-12-27DOI: 10.21608/ejh.2020.50821.1387
Hend Ibrahim Abd Alhalim, Naglaa I. Sarhan, E. Laag
Introduction: Current treatment strategies of osteoarthritis (OA) are restricted to short-term symptomatic relief by medical therapy. There is increasing evidence that bone marrow derived mesenchymal stem cells (BM-MSCs) represent a novel treatment for cartilage regeneration.Aim of the Work is to evaluate the therapeutic effect of intra-articular injection of BM-MSCs on the experimentally induced OA in the knee joint of adult male albino rat. Materials and Methods: fifty five adult male albino rats were used and divided into five groups. Group I served as a control group. Group II for harvesting BM-MSCs from the tibia and femur. Group III for induction of OA by intra-articular injection of monosodium iodoacetate (MIA) to be sacrificed after 2 weeks from induction. Group IV: Hyaluronic acid (HA) was injected in both normal knees to be examined after 4 and 8 weeks of injection of HA. Group V: Knee joint OA was induced as in group III. After 2 weeks, rats were injected with BM-MSCs suspended in HA (MSCs HA) in their knees. Knee joint specimens were examined after 4 and 8 weeks of MSCs-HA injection. Knee joint specimens were processed for histological study and stained with Hematoxylin and eosin, Mallory trichrome and fast green Safranin O stains. For immunohistochemical study, the number of CD44 positive chondrocytes was evaluated and the obtained morphological data were statistically analyzed. Results: OA knees treated with MSCs-HA showed well evidenced repair of the articular cartilage by hyaline-like cartilage in association with significant increase in the number of CD44 positive chondrocytes. Conclusion: Intra-articular injection of in vitro expanded BM-MSCs suspended in HA promotes articular cartilage repair by hyaline-like cartilage in MIA induced OA.
{"title":"A Histological Study on The Effect of Bone Marrow Derived Mesenchymal Stem Cells Suspended in Hyaluronic Acid on Articular Cartilage of Osteoarthritic Knee joint of Adult Male Albino Rat","authors":"Hend Ibrahim Abd Alhalim, Naglaa I. Sarhan, E. Laag","doi":"10.21608/ejh.2020.50821.1387","DOIUrl":"https://doi.org/10.21608/ejh.2020.50821.1387","url":null,"abstract":"Introduction: Current treatment strategies of osteoarthritis (OA) are restricted to short-term symptomatic relief by medical therapy. There is increasing evidence that bone marrow derived mesenchymal stem cells (BM-MSCs) represent a novel treatment for cartilage regeneration.Aim of the Work is to evaluate the therapeutic effect of intra-articular injection of BM-MSCs on the experimentally induced OA in the knee joint of adult male albino rat. Materials and Methods: fifty five adult male albino rats were used and divided into five groups. Group I served as a control group. Group II for harvesting BM-MSCs from the tibia and femur. Group III for induction of OA by intra-articular injection of monosodium iodoacetate (MIA) to be sacrificed after 2 weeks from induction. Group IV: Hyaluronic acid (HA) was injected in both normal knees to be examined after 4 and 8 weeks of injection of HA. Group V: Knee joint OA was induced as in group III. After 2 weeks, rats were injected with BM-MSCs suspended in HA (MSCs HA) in their knees. Knee joint specimens were examined after 4 and 8 weeks of MSCs-HA injection. Knee joint specimens were processed for histological study and stained with Hematoxylin and eosin, Mallory trichrome and fast green Safranin O stains. For immunohistochemical study, the number of CD44 positive chondrocytes was evaluated and the obtained morphological data were statistically analyzed. Results: OA knees treated with MSCs-HA showed well evidenced repair of the articular cartilage by hyaline-like cartilage in association with significant increase in the number of CD44 positive chondrocytes. Conclusion: Intra-articular injection of in vitro expanded BM-MSCs suspended in HA promotes articular cartilage repair by hyaline-like cartilage in MIA induced OA.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"42 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81561389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-15DOI: 10.21608/ejh.2020.46863.1376
Eman S El-Roghy, R. Yassien
Background: Cadmium is a common toxic heavy metal of great concern for environmental pollution and human health. It is extensively used in many industries as radiation screens, rechargeable batteries and pigments. Montelukast is a powerful highly effective anti-inflammatory drug. Recently it shows significant antioxidant properties.Aim of the Study: evaluate the effect of Cadmium on thyroid glands and to assess the possible protective role of MontelukastMaterials and Methods: Forty adult male albino rats were divided into four equal groups received treatment for 6 weeks: (I) (Control group) received distilled water. (II) (Cadmium group)"3mg/kg". (III) (Recovery group) kept untreated for another 6 weeks after Cadmium administration with the same dose and period as pervious group. (IV) (Montelukast and Cadmium group) received Montelukast "10mg/kg" and Cadmium"3mg/kg". At the end, all animals were sacrificed. Thyroid gland samples were obtained for histological and immunohistochemical studies. Blood samples were obtained for hormonal assay. Data was statistically analyzed.Results: Histological examination of thyroid glands of Cadmium treated rat’s showed disorganization and irregularity in the follicles. The follicular cells appeared distorted with pyknotic nuclei and some cell desquamated in follicular lumen. Colloid retraction within the follicles, cellular infiltration around congested blood vessels and marked collagen fibers deposition in connective tissue septae were detected. Significant increase in Caspase-3 immune expression in the follicular cell’s cytoplasm was noticed. High significant decrease of thyroid hormone (T3, T4) level was noticed. These changes were still seen in the recovery group. Co-administration of Montelukast showed obvious protection against the hazardous effects of Cadmium. Conclusion: Cadmium induces marked thyroid tissue damage via, fibrosis and apoptosis. These structural changes can be alleviated by combined treatment with Montelukast.
{"title":"Role of Montelukast in Alleviation of Toxicity Induced by Cadmium in Thyroid Gland of Adult Male Albino Rats. (Histological, Immunohistochemical and Biochemical Study)","authors":"Eman S El-Roghy, R. Yassien","doi":"10.21608/ejh.2020.46863.1376","DOIUrl":"https://doi.org/10.21608/ejh.2020.46863.1376","url":null,"abstract":"Background: Cadmium is a common toxic heavy metal of great concern for environmental pollution and human health. It is extensively used in many industries as radiation screens, rechargeable batteries and pigments. Montelukast is a powerful highly effective anti-inflammatory drug. Recently it shows significant antioxidant properties.Aim of the Study: evaluate the effect of Cadmium on thyroid glands and to assess the possible protective role of MontelukastMaterials and Methods: Forty adult male albino rats were divided into four equal groups received treatment for 6 weeks: (I) (Control group) received distilled water. (II) (Cadmium group)\"3mg/kg\". (III) (Recovery group) kept untreated for another 6 weeks after Cadmium administration with the same dose and period as pervious group. (IV) (Montelukast and Cadmium group) received Montelukast \"10mg/kg\" and Cadmium\"3mg/kg\". At the end, all animals were sacrificed. Thyroid gland samples were obtained for histological and immunohistochemical studies. Blood samples were obtained for hormonal assay. Data was statistically analyzed.Results: Histological examination of thyroid glands of Cadmium treated rat’s showed disorganization and irregularity in the follicles. The follicular cells appeared distorted with pyknotic nuclei and some cell desquamated in follicular lumen. Colloid retraction within the follicles, cellular infiltration around congested blood vessels and marked collagen fibers deposition in connective tissue septae were detected. Significant increase in Caspase-3 immune expression in the follicular cell’s cytoplasm was noticed. High significant decrease of thyroid hormone (T3, T4) level was noticed. These changes were still seen in the recovery group. Co-administration of Montelukast showed obvious protection against the hazardous effects of Cadmium. Conclusion: Cadmium induces marked thyroid tissue damage via, fibrosis and apoptosis. These structural changes can be alleviated by combined treatment with Montelukast.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"68 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75635418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.21608/ejh.2020.47682.1380
S. M. Mohamed, K. El-Gohari, Hany W Abd El-Malak, S. Kamar
Background: Vitamin D3 has the potential to modulate both endocrine and immune system. Evidence increased on non-classical role of vitamin D in many autoimmune diseases. Vitamin D deficiency is considered as a global health problem and has been suggested based on animal studies to be associated with prevalence of glucose intolerance.Aim: Investigate the effect of vit. D deficiency on the blood glucose level and the structure of pancreas of albino rat.Material and methods:Thirty adult male, Sprague Dawley, albino rats were used in the current study, and were divided into two groups; Group I (Control) was fed vit. D balanced diet and exposed to sun light, and Group II was fed vit. D deficient diet and deprived from sun light exposure. Group II was further subdivided into IIa which was sacrificed after 1 month and IIb which was sacrificed after 2 months. Pancreata were collected from all group and prepared for histopathological examination. Morphometric measurements were done using image analyzer and blood glucose level was measured and all data were statistically analyzed. Results: Group IIa showed fewer and smaller islets of Langerhans compared with those of the control group while Group IIb showed marked decrease in the number and size of islets of Langerhans with disfigurement. Blood glucose level of group IIb showed highly significant increase compared to the control.Conclusion: there is an inverse correlation between serum vitamin D and insulin resistance. Persistent vitamin D deficiency status may lead to metabolic disorders including insulin resistance and raised blood glucose.
{"title":"Role Of Vitamin D Deficiency On Induction Of Diabetes in Albino Rats","authors":"S. M. Mohamed, K. El-Gohari, Hany W Abd El-Malak, S. Kamar","doi":"10.21608/ejh.2020.47682.1380","DOIUrl":"https://doi.org/10.21608/ejh.2020.47682.1380","url":null,"abstract":"Background: Vitamin D3 has the potential to modulate both endocrine and immune system. Evidence increased on non-classical role of vitamin D in many autoimmune diseases. Vitamin D deficiency is considered as a global health problem and has been suggested based on animal studies to be associated with prevalence of glucose intolerance.Aim: Investigate the effect of vit. D deficiency on the blood glucose level and the structure of pancreas of albino rat.Material and methods:Thirty adult male, Sprague Dawley, albino rats were used in the current study, and were divided into two groups; Group I (Control) was fed vit. D balanced diet and exposed to sun light, and Group II was fed vit. D deficient diet and deprived from sun light exposure. Group II was further subdivided into IIa which was sacrificed after 1 month and IIb which was sacrificed after 2 months. Pancreata were collected from all group and prepared for histopathological examination. Morphometric measurements were done using image analyzer and blood glucose level was measured and all data were statistically analyzed. Results: Group IIa showed fewer and smaller islets of Langerhans compared with those of the control group while Group IIb showed marked decrease in the number and size of islets of Langerhans with disfigurement. Blood glucose level of group IIb showed highly significant increase compared to the control.Conclusion: there is an inverse correlation between serum vitamin D and insulin resistance. Persistent vitamin D deficiency status may lead to metabolic disorders including insulin resistance and raised blood glucose.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"56 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79164341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-05DOI: 10.21608/ejh.2020.46113.1373
R. Yassien, O. Zedan, N. Ghoneim
Background: Retinal degenerative diseases are induced by many environmental and genetic agents and may lead to permanent blindness. Induced retinal degeneration by sodium iodate (NaIO3) is widely used to study mechanism of retinal cell death. Silymarin are flavonoids which has powerful antioxidant effects.Aim of work: Study the effects of NaIO3 on the retina of adult male albino rat and possible protective role of silymarin.Material and methods: Forty adult male albino rats were used, they were divided equally to four groups: group I (control), group II (silymarin), group III (NaIO3) and group IV (silymarin and NaIO3). At end of experiment, retina of the whole groups was prepared for histological and immunohistochemical studies. Paraffin sections were stained by hematoxylin and eosin (H&E), Glial fibrillary acidic protein (GFAP), Caspase3 and inducible nitric oxide synthase (iNOS) immunostaining. Morphometric measurements were done and data was statistically analyzed.Results: H &E sections of GIII revealed thinning, disorganization and loss of most layers of retina. Degeneration of retinal pigmented epithelium, damage of photoreceptors, necrosis and loss of cells of outer, inner nuclear layers and ganglion cell layers were noticed. Loss of reticular appearance, thinning of outer and inner plexiform layers and congested blood vessels in many layers were detected. GFAP, Caspase3 and iNOS revealed strong positive immune reaction in this group. Group IV showed marked improvement of retina but some changes were still seen. Electron microscope (EM) and morphometric results confirmed light microscopic results.Conclusion: Sodium iodate has destructive effects on all layers of retina and preceding silymarin has protective role.
{"title":"Histological and immunohistochemical study on the effect of sodium iodate on the retina of adult male albino rat and the possible protective role of silymarin","authors":"R. Yassien, O. Zedan, N. Ghoneim","doi":"10.21608/ejh.2020.46113.1373","DOIUrl":"https://doi.org/10.21608/ejh.2020.46113.1373","url":null,"abstract":"Background: Retinal degenerative diseases are induced by many environmental and genetic agents and may lead to permanent blindness. Induced retinal degeneration by sodium iodate (NaIO3) is widely used to study mechanism of retinal cell death. Silymarin are flavonoids which has powerful antioxidant effects.Aim of work: Study the effects of NaIO3 on the retina of adult male albino rat and possible protective role of silymarin.Material and methods: Forty adult male albino rats were used, they were divided equally to four groups: group I (control), group II (silymarin), group III (NaIO3) and group IV (silymarin and NaIO3). At end of experiment, retina of the whole groups was prepared for histological and immunohistochemical studies. Paraffin sections were stained by hematoxylin and eosin (H&E), Glial fibrillary acidic protein (GFAP), Caspase3 and inducible nitric oxide synthase (iNOS) immunostaining. Morphometric measurements were done and data was statistically analyzed.Results: H &E sections of GIII revealed thinning, disorganization and loss of most layers of retina. Degeneration of retinal pigmented epithelium, damage of photoreceptors, necrosis and loss of cells of outer, inner nuclear layers and ganglion cell layers were noticed. Loss of reticular appearance, thinning of outer and inner plexiform layers and congested blood vessels in many layers were detected. GFAP, Caspase3 and iNOS revealed strong positive immune reaction in this group. Group IV showed marked improvement of retina but some changes were still seen. Electron microscope (EM) and morphometric results confirmed light microscopic results.Conclusion: Sodium iodate has destructive effects on all layers of retina and preceding silymarin has protective role.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"62 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85277403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.21608/EJH.2020.24868.1256
Amany I. Ahmed, Soad Abd-Elgwwad, R. Hasan, Mona M. Abd-Elgalil
Introduction: Gastric ulcer is a complex disease affecting the stomach. Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are the most common cause of gastric ulcers. Drugs used in gastric treatment induce side effects and drug interactions. Portulaca oleracea L. (purslane), Foeniculum Vulgare Mill (Fennel) are herbal remedies having gastro-protective effects.Aim of work: To assess the cytoprotective effects of trio-clar versus fennel and ∕or purslane on aspirin-induced gastric ulcer in adult female albino rats.Materials and Methods: Forty-two adult female albino rats were divided into three main groups. Group I (control group). Group II (gastric ulcer), overnight fasted rats received aspirin solution (300 mg/kg/day) orally by gastric tube. GroupIIIincorporated as the treated gastric ulcer group that was subdivided equally into four subgroups. GIIIa: gastric ulcer treated with Trio-clar. GIIIb: gastric ulcer treated with purslane extract at dose 750 mg ∕ kg. GIIIc: gastric ulcer treated with fennel extract at a dose of 300 mg ∕ kg. GIIId: gastric ulcer treated with a combined dose of purslane and fennel extract. Fundus of stomach specimens was prepared and examined using different histological techniques.Results: Light microscopic study of the gastric ulcer group depicted severe mucosal injury in the form of ulcerative erosion, dilated distorted glandular architecture, damage of parietal and chief cells with vacuolated cytoplasm and pyknotic nuclei, congested blood vessels and inflammatory cellular infiltration. A reduction of combined Alcian blue and Periodic-Acid-Schiff reaction coupled to a significant increase in area percentage of collagen fibers observed upon aspirin administration. The ultrastructural assessment confirmed these distortions. The treated groups showed different degrees of improvement in the histological changes depicted previously with priority to the combined administration of purslane and fennel extracts.Conclusion: Combined administration of purslane and fennel was more effective in enhancing the healing process than either purslane, fennel alone or trio-clar drug.
{"title":"Trio-clar Versus Portulaca Oleracea and/or Foeniculum Vulgare Extracts on Induced Gastric Ulcer in Adult Female Albino Rats. A Histological Study","authors":"Amany I. Ahmed, Soad Abd-Elgwwad, R. Hasan, Mona M. Abd-Elgalil","doi":"10.21608/EJH.2020.24868.1256","DOIUrl":"https://doi.org/10.21608/EJH.2020.24868.1256","url":null,"abstract":"Introduction: Gastric ulcer is a complex disease affecting the stomach. Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are the most common cause of gastric ulcers. Drugs used in gastric treatment induce side effects and drug interactions. Portulaca oleracea L. (purslane), Foeniculum Vulgare Mill (Fennel) are herbal remedies having gastro-protective effects.Aim of work: To assess the cytoprotective effects of trio-clar versus fennel and ∕or purslane on aspirin-induced gastric ulcer in adult female albino rats.Materials and Methods: Forty-two adult female albino rats were divided into three main groups. Group I (control group). Group II (gastric ulcer), overnight fasted rats received aspirin solution (300 mg/kg/day) orally by gastric tube. GroupIIIincorporated as the treated gastric ulcer group that was subdivided equally into four subgroups. GIIIa: gastric ulcer treated with Trio-clar. GIIIb: gastric ulcer treated with purslane extract at dose 750 mg ∕ kg. GIIIc: gastric ulcer treated with fennel extract at a dose of 300 mg ∕ kg. GIIId: gastric ulcer treated with a combined dose of purslane and fennel extract. Fundus of stomach specimens was prepared and examined using different histological techniques.Results: Light microscopic study of the gastric ulcer group depicted severe mucosal injury in the form of ulcerative erosion, dilated distorted glandular architecture, damage of parietal and chief cells with vacuolated cytoplasm and pyknotic nuclei, congested blood vessels and inflammatory cellular infiltration. A reduction of combined Alcian blue and Periodic-Acid-Schiff reaction coupled to a significant increase in area percentage of collagen fibers observed upon aspirin administration. The ultrastructural assessment confirmed these distortions. The treated groups showed different degrees of improvement in the histological changes depicted previously with priority to the combined administration of purslane and fennel extracts.Conclusion: Combined administration of purslane and fennel was more effective in enhancing the healing process than either purslane, fennel alone or trio-clar drug.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":" 4","pages":"1008-1033"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91410952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.21608/ejh.2019.9692.1087
Soad Jimmy, Dalia M. Elmarakby, Maha M. Abo Gazia, A. Morsy
Introduction: Partial hepatectomy is indicated as the main curative approach for traumatic rupture of the hepatic parenchyma, and hepatocellular carcinoma. Hepatic stellate cells (HSCs) are vital and have a significant importance in their emerging role in hepatic regeneration.Aim of the Work: Study the role of hepatic stellate cells and angiogenesis in liver regeneration after 70% partial hepatectomy in adult male albino rats.Materials and Methods: Forty adult male albino rats(4-6months) weighing 150-200 gm were randomized into 5 groups of eight rats each; group I was the sham control group, and the animals were subjected to 70% partial hepatectomy in groups II, III, IV and V in which they were postoperatively sacrificed 3, 7, 14 and 21 days respectively. At the time of scarification specimens were taken for histological and immunohistochemical studies. H and E and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF) and α-smooth muscle actin (α- SMA) were done. Image analysis and statistical analysis of the obtained results were performed.Results: The liver showed disturbed histological architecture with distortion of the neatly arranged hepatocyte plates in group II which was restored in the following groups. Wide distribution of Kupffer cells, was also noted in group II and continued in group III. The PCNA and VEGF activity showed significant positive reaction in groups II and III , insignificant positive immunoreaction was reported in groups IV and V . The α- SMA showed significant positive reaction in group II, insignificant immunoreaction was reported in the remaining other groups.Conclusion: Partial hepatectomy stimulated liver regeneration in the remaining liver lobes with reconstitution of most of the liver mass 21 days postoperatively. This regenerative process was associated with angiogenesis and HSCs activation as denoted by VEGF expression and α- SMA expression respectively.
{"title":"The Role of Hepatic Stellate Cells and Angiogenesis in Liver Regeneration Following Partial Hepatectomy in Adult Male Albino Rats: Histological and Immunohistochemical Study","authors":"Soad Jimmy, Dalia M. Elmarakby, Maha M. Abo Gazia, A. Morsy","doi":"10.21608/ejh.2019.9692.1087","DOIUrl":"https://doi.org/10.21608/ejh.2019.9692.1087","url":null,"abstract":"Introduction: Partial hepatectomy is indicated as the main curative approach for traumatic rupture of the hepatic parenchyma, and hepatocellular carcinoma. Hepatic stellate cells (HSCs) are vital and have a significant importance in their emerging role in hepatic regeneration.Aim of the Work: Study the role of hepatic stellate cells and angiogenesis in liver regeneration after 70% partial hepatectomy in adult male albino rats.Materials and Methods: Forty adult male albino rats(4-6months) weighing 150-200 gm were randomized into 5 groups of eight rats each; group I was the sham control group, and the animals were subjected to 70% partial hepatectomy in groups II, III, IV and V in which they were postoperatively sacrificed 3, 7, 14 and 21 days respectively. At the time of scarification specimens were taken for histological and immunohistochemical studies. H and E and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF) and α-smooth muscle actin (α- SMA) were done. Image analysis and statistical analysis of the obtained results were performed.Results: The liver showed disturbed histological architecture with distortion of the neatly arranged hepatocyte plates in group II which was restored in the following groups. Wide distribution of Kupffer cells, was also noted in group II and continued in group III. The PCNA and VEGF activity showed significant positive reaction in groups II and III , insignificant positive immunoreaction was reported in groups IV and V . The α- SMA showed significant positive reaction in group II, insignificant immunoreaction was reported in the remaining other groups.Conclusion: Partial hepatectomy stimulated liver regeneration in the remaining liver lobes with reconstitution of most of the liver mass 21 days postoperatively. This regenerative process was associated with angiogenesis and HSCs activation as denoted by VEGF expression and α- SMA expression respectively.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"53 1","pages":"1070-1095"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79980224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-20DOI: 10.21608/ejh.2020.48021.1381
Hanan E.L. Mokhtar, A. Sewelam
Background: Despite worldwide use of monosodium glutamate (MSG) as a flavor enhancer, its consumption has been an alarm due to daily exposure without definitive safety or exact limit. Vitamin C has an antioxidant activity. Objective: to clarify effects of neonatal MSG intake on heart structure of male albino rat pups and the possible modifying role of vitamin C and to assess reversibility of these effects.Material and Methods: 40 male albino rats at postnatal day 1(PD1) were allocated to three groups; control, group B administered MSG (4 mg/gm bw/day) and group C administered previous dose of MSG plus vitamin C (500 mg/kg bw/day) for 10 days. Groups B and C were subdivided according to animal sacrification age into B1, C1 sacrificed at PD10 (after the last dose of treatment) and B2, C2 sacrificed at PD30 (20 days after treatment cessation). At experimental end, left ventricular specimens were processed for histopathological, immunohistochemical and morphometric studies. The Results: Histologically, group B1 revealed massive degenerative changes including marked muscle fibers disruption, degeneration, separation with increased CT cells and fibroblasts, dilated congested capillaries and extravasated hemorrhage. Cardiomyocytes showed nuclear pyknosis, cytoplasmic coagulative necrosis and vacuolation. Previous changes were still present in group B2 but with less degree. MSG plus vitamin C resulted in greatly restored normal cardiomyocyte architecture in group C1 but complete recovery was observed in group C2. The mean area percentage of collagen fibers, vimentin and iNOS expressions showed high significant increase in B1, B2, low significant elevation in C1 but non-significantly different in C2 relative to control group. Conclusion: MSG induced myocardial toxicity in early postnatal period. Vitamin C greatly ameliorated histopathological and immunohistochemical alterations in cardiac tissue. MSG withdrawal for 20 days showed mild improvement but withdrawal after vitamin C administration was more effective in reversibility of MSG toxicity.
{"title":"Impact of Monosodium Glutamate Intake on Heart Structure of Neonate Albino Rats and The Protective Role of Vitamin C","authors":"Hanan E.L. Mokhtar, A. Sewelam","doi":"10.21608/ejh.2020.48021.1381","DOIUrl":"https://doi.org/10.21608/ejh.2020.48021.1381","url":null,"abstract":"Background: Despite worldwide use of monosodium glutamate (MSG) as a flavor enhancer, its consumption has been an alarm due to daily exposure without definitive safety or exact limit. Vitamin C has an antioxidant activity. Objective: to clarify effects of neonatal MSG intake on heart structure of male albino rat pups and the possible modifying role of vitamin C and to assess reversibility of these effects.Material and Methods: 40 male albino rats at postnatal day 1(PD1) were allocated to three groups; control, group B administered MSG (4 mg/gm bw/day) and group C administered previous dose of MSG plus vitamin C (500 mg/kg bw/day) for 10 days. Groups B and C were subdivided according to animal sacrification age into B1, C1 sacrificed at PD10 (after the last dose of treatment) and B2, C2 sacrificed at PD30 (20 days after treatment cessation). At experimental end, left ventricular specimens were processed for histopathological, immunohistochemical and morphometric studies. The Results: Histologically, group B1 revealed massive degenerative changes including marked muscle fibers disruption, degeneration, separation with increased CT cells and fibroblasts, dilated congested capillaries and extravasated hemorrhage. Cardiomyocytes showed nuclear pyknosis, cytoplasmic coagulative necrosis and vacuolation. Previous changes were still present in group B2 but with less degree. MSG plus vitamin C resulted in greatly restored normal cardiomyocyte architecture in group C1 but complete recovery was observed in group C2. The mean area percentage of collagen fibers, vimentin and iNOS expressions showed high significant increase in B1, B2, low significant elevation in C1 but non-significantly different in C2 relative to control group. Conclusion: MSG induced myocardial toxicity in early postnatal period. Vitamin C greatly ameliorated histopathological and immunohistochemical alterations in cardiac tissue. MSG withdrawal for 20 days showed mild improvement but withdrawal after vitamin C administration was more effective in reversibility of MSG toxicity.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"1 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81892612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-20DOI: 10.21608/ejh.2020.42968.1357
S. Mohammed
{"title":"Modulatory effect of bone marrow-derived stem cells against Tamoxifen induced liver injury in albino rats: A Histological, Immunohistochemical and Biochemical study","authors":"S. Mohammed","doi":"10.21608/ejh.2020.42968.1357","DOIUrl":"https://doi.org/10.21608/ejh.2020.42968.1357","url":null,"abstract":"","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"114 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79858591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-19DOI: 10.21608/ejh.2020.45572.1370
N. Afifi, Ismail Shebl
Odontogenic keratocyst (OKC) is one of the debatable odontogenic lesions. The need for more researches in order to determine the true nature of this lesion was one of the WHO’s recommendations in 2016. Stromal reactions to epithelial neoplasms are marked by the appearance of alpha smooth muscle actin (α-SMA) positive myofibroblasts (MFs). Stromal collagen has a vital role in pathogenesis of odontogenic lesions. Objectives: to compare the count of α-SMA positive MFs, collagen quality and packing in OKC, in relation to dentigerous cyst and ameloblastoma. Material and methods: α-SMA immunohistochemically positive MFs count, as well as the fraction of yellow-green fibers to detect the collagen quality and packing using picrosirius red staining with polarized light microscope, were compared in 11 samples of each of ameloblastoma, OKC and dentigerous cyst. Statistical analysis was performed. Results: the average number of MFs were 53.7, 41.9 and 11.3 in ameloblastoma, OKC and dentigerous cysts respectively with statistically significant differences (P-value
{"title":"Role of myofibroblasts and collagen quality in elaboration of debatable nature of odontogenic keratocyst","authors":"N. Afifi, Ismail Shebl","doi":"10.21608/ejh.2020.45572.1370","DOIUrl":"https://doi.org/10.21608/ejh.2020.45572.1370","url":null,"abstract":"Odontogenic keratocyst (OKC) is one of the debatable odontogenic lesions. The need for more researches in order to determine the true nature of this lesion was one of the WHO’s recommendations in 2016. Stromal reactions to epithelial neoplasms are marked by the appearance of alpha smooth muscle actin (α-SMA) positive myofibroblasts (MFs). Stromal collagen has a vital role in pathogenesis of odontogenic lesions. Objectives: to compare the count of α-SMA positive MFs, collagen quality and packing in OKC, in relation to dentigerous cyst and ameloblastoma. Material and methods: α-SMA immunohistochemically positive MFs count, as well as the fraction of yellow-green fibers to detect the collagen quality and packing using picrosirius red staining with polarized light microscope, were compared in 11 samples of each of ameloblastoma, OKC and dentigerous cyst. Statistical analysis was performed. Results: the average number of MFs were 53.7, 41.9 and 11.3 in ameloblastoma, OKC and dentigerous cysts respectively with statistically significant differences (P-value","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"49 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83224027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-18DOI: 10.21608/ejh.2020.43345.1359
S. Saad, E. Eldeen, A. Soliman, R. Din, G. Hanna
Background: Skeletal muscle injuries are one of the most common injuries in sport medicine. Many injection protocols have been proposed for the treatment as corticosteroid injection. Recently prolotherapy appears to have a safety profile comparable with other injection procedures. Aim of the work: was to determine the efficacy of dextrose prolotherapy in treatment of skeletal muscle injury in adult male albino rats.Material and methods: Sixty three adult male albino rats were used in the study. They were divided into control (group I). Group II was divided into sham operated group (group IIA) and muscle injury group (group IIB) and group III was divided into lidocaine injected group (group IIIA ) and muscle injury treated with dextrose prolotherapy group (group IIIB). Muscle specimens were taken at 5,12 and 28 days and processed for light microscopeResults: Examination of Group IIB1 (5 days) showed intense infiltration of mononucleated inflammatory cells intermingling with dispersed myoblasts and macrophages. Group IIB2 (12 days) showed regenerating myotubes intermingling with mononuclear inflammatory infiltrate and macrophages. Group IIB3 (28 days) showed some muscle fibers with peripherally elongated nuclei while others showed centrally vesicular ones. Examination of group IIIB1 (5 day treated) showed longitudinal regenerating myofibers with multiple rows of internal vesiculated nuclei. Group IIIB2 (12 days treated) showed newly formed myofibers with incomplete striations together with well developed newly formed striated longitudinal muscle bundles with peripheral flattened nuclei ,group IIIB3 (28 days treated) showed cross striated muscle fibers with the appearance of elongated vesicular nuclei.Conclusion: dextrose prolotherapy was effective in soft tissue healing as it accelerated myoblast proliferation and differentiation.
{"title":"A Histological Study on the Effect of Dextrose Prolotherapy on Skeletal Muscle Injury in Adult Male Albino Rats.","authors":"S. Saad, E. Eldeen, A. Soliman, R. Din, G. Hanna","doi":"10.21608/ejh.2020.43345.1359","DOIUrl":"https://doi.org/10.21608/ejh.2020.43345.1359","url":null,"abstract":"Background: Skeletal muscle injuries are one of the most common injuries in sport medicine. Many injection protocols have been proposed for the treatment as corticosteroid injection. Recently prolotherapy appears to have a safety profile comparable with other injection procedures. Aim of the work: was to determine the efficacy of dextrose prolotherapy in treatment of skeletal muscle injury in adult male albino rats.Material and methods: Sixty three adult male albino rats were used in the study. They were divided into control (group I). Group II was divided into sham operated group (group IIA) and muscle injury group (group IIB) and group III was divided into lidocaine injected group (group IIIA ) and muscle injury treated with dextrose prolotherapy group (group IIIB). Muscle specimens were taken at 5,12 and 28 days and processed for light microscopeResults: Examination of Group IIB1 (5 days) showed intense infiltration of mononucleated inflammatory cells intermingling with dispersed myoblasts and macrophages. Group IIB2 (12 days) showed regenerating myotubes intermingling with mononuclear inflammatory infiltrate and macrophages. Group IIB3 (28 days) showed some muscle fibers with peripherally elongated nuclei while others showed centrally vesicular ones. Examination of group IIIB1 (5 day treated) showed longitudinal regenerating myofibers with multiple rows of internal vesiculated nuclei. Group IIIB2 (12 days treated) showed newly formed myofibers with incomplete striations together with well developed newly formed striated longitudinal muscle bundles with peripheral flattened nuclei ,group IIIB3 (28 days treated) showed cross striated muscle fibers with the appearance of elongated vesicular nuclei.Conclusion: dextrose prolotherapy was effective in soft tissue healing as it accelerated myoblast proliferation and differentiation.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"74 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77486037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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