Pub Date : 2021-01-29DOI: 10.21608/EJH.2021.52917.1398
M. S. Alam, Ataur Rahman, Aminul Islam, Abu N. M. A. Rahman
Objective: The purpose of this research was to observe the age-related growth and histomorphological changes in Japanese quail testes from post-hatching to sexual maturity as well as spermatogenesis.Materials and Methods: After collection, the testes weight, length, width, and index analysis were performed, and histomorphological, histological and immunohistochemical observations were conducted using hematoxylin-eosin, PAS-hematoxylin, and anti-Hsc70t staining, respectively at different ages.Results: The weight of the testes increased gradually with age and reached a peak at 70 days. The length and width of the testes were maximal at 70 days. The testis index was positively correlated with age and body weight (BW) toward sexual maturity. Histologically, at 15 days of age, the seminiferous tubules (STs) remained in immature state with undifferentiated spermatogenic cells of only one to two layers of epithelial cells. At 28 days, the spermatogenic cells were differentiated from spermatogonia to spermatocytes. At 42 days, remarkable enlargement of STs with a series of spermatogenic cell development up to the round spermatids and Leydig cells in the interstitial region. Finally, at 70 days, extremely enlarged STs containing all stages of spermatogenic cells. The diameter and height of STs were maximal at 70 days than that of 42 days of age. A strong Hsc 70t immunoreaction was found in the round to elongated spermatids/spermatozoa near to the lumen of STs, clearly indicating well-characterized spermatogenesis in quail testis at 70 days of age.Conclusion: The findings may contribute to our understanding of quail’s spermatology and provide basic knowledge for reproductive toxicology, physiology and pathology studies.
{"title":"Growth of Japanese Quail Testes in Relation to Age: Morphological and Immunohistochemical Observation","authors":"M. S. Alam, Ataur Rahman, Aminul Islam, Abu N. M. A. Rahman","doi":"10.21608/EJH.2021.52917.1398","DOIUrl":"https://doi.org/10.21608/EJH.2021.52917.1398","url":null,"abstract":"Objective: The purpose of this research was to observe the age-related growth and histomorphological changes in Japanese quail testes from post-hatching to sexual maturity as well as spermatogenesis.Materials and Methods: After collection, the testes weight, length, width, and index analysis were performed, and histomorphological, histological and immunohistochemical observations were conducted using hematoxylin-eosin, PAS-hematoxylin, and anti-Hsc70t staining, respectively at different ages.Results: The weight of the testes increased gradually with age and reached a peak at 70 days. The length and width of the testes were maximal at 70 days. The testis index was positively correlated with age and body weight (BW) toward sexual maturity. Histologically, at 15 days of age, the seminiferous tubules (STs) remained in immature state with undifferentiated spermatogenic cells of only one to two layers of epithelial cells. At 28 days, the spermatogenic cells were differentiated from spermatogonia to spermatocytes. At 42 days, remarkable enlargement of STs with a series of spermatogenic cell development up to the round spermatids and Leydig cells in the interstitial region. Finally, at 70 days, extremely enlarged STs containing all stages of spermatogenic cells. The diameter and height of STs were maximal at 70 days than that of 42 days of age. A strong Hsc 70t immunoreaction was found in the round to elongated spermatids/spermatozoa near to the lumen of STs, clearly indicating well-characterized spermatogenesis in quail testis at 70 days of age.Conclusion: The findings may contribute to our understanding of quail’s spermatology and provide basic knowledge for reproductive toxicology, physiology and pathology studies.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"4 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78797158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.21608/EJH.2021.56052.1410
Ebtesam M Abdurabbah, Y. Mahmoud, Nawara Belgasim, N. Fares, S. Mousa, Faten S. Abo-Zeid
Background: Camel milk is an excellent source of nutrients and has medicinal importance for human in many countries all over the world especially in nomadic societies of Arab countries. Staphylococcus aureus (S. aureus) is instinctively present in milk and dairy products, and associated with many outbreaks. Milk is a good substrate for S. aureus rise and toxin production. When transmitted to human body this pathogen may affect skin, brain, kidney, liver and several other vital organs. Therefore, the pathological effects of S. aureus have been the focus of several recent research works.Aim: The present investigation was designed to better characterize the histopathological outcomes of contaminated raw camel milk with S. aureus on the mice liver. Materials and methods: S. aureus were isolated from specimens of lactating she-camels (Camelus dromedarius), weighing 300-540 kg, in Libya and identified using microbiological and molecular techniques. Twenty healthy male Swiss albino mice, weighing 22-25 gm, were divided into two groups; Group I (control) and Group II (orally injected mice with a single dose of aqueous solutions of the isolated bacteria at a concentration of 5x108/0.1ml). Animals sacrificed after three days of oral feeding with milk containing high bacterial load and their livers were dissected out for macro- and microscopic inspection.Results: Our study reported that infection with contaminated milk causes outer liquid-filled liver abscesses. Furthermore, various histopathological changes could be detected. Conclusion: This study may highlight the potential risk of consuming raw she-camel milk, especially upon lack of strict hygienic and preventative measures to avoid the presence of S. aureus in milk.
{"title":"Outcomes of albino Mice Liver Infected with Contaminated Camel Milk","authors":"Ebtesam M Abdurabbah, Y. Mahmoud, Nawara Belgasim, N. Fares, S. Mousa, Faten S. Abo-Zeid","doi":"10.21608/EJH.2021.56052.1410","DOIUrl":"https://doi.org/10.21608/EJH.2021.56052.1410","url":null,"abstract":"Background: Camel milk is an excellent source of nutrients and has medicinal importance for human in many countries all over the world especially in nomadic societies of Arab countries. Staphylococcus aureus (S. aureus) is instinctively present in milk and dairy products, and associated with many outbreaks. Milk is a good substrate for S. aureus rise and toxin production. When transmitted to human body this pathogen may affect skin, brain, kidney, liver and several other vital organs. Therefore, the pathological effects of S. aureus have been the focus of several recent research works.Aim: The present investigation was designed to better characterize the histopathological outcomes of contaminated raw camel milk with S. aureus on the mice liver. Materials and methods: S. aureus were isolated from specimens of lactating she-camels (Camelus dromedarius), weighing 300-540 kg, in Libya and identified using microbiological and molecular techniques. Twenty healthy male Swiss albino mice, weighing 22-25 gm, were divided into two groups; Group I (control) and Group II (orally injected mice with a single dose of aqueous solutions of the isolated bacteria at a concentration of 5x108/0.1ml). Animals sacrificed after three days of oral feeding with milk containing high bacterial load and their livers were dissected out for macro- and microscopic inspection.Results: Our study reported that infection with contaminated milk causes outer liquid-filled liver abscesses. Furthermore, various histopathological changes could be detected. Conclusion: This study may highlight the potential risk of consuming raw she-camel milk, especially upon lack of strict hygienic and preventative measures to avoid the presence of S. aureus in milk.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"7 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87848871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.21608/EJH.2021.55093.1404
M. Hussein, Xiaojuan Cao, Yang Li, Mei Jie
Loach (Misgurnus anguillicaudatus) is an essential commercial fish in China. We aimed to give some notes on brain histology of loach fish and characterize the sites of stem cells in the brain. As well as, we showed the sites of proliferating cells with detection of their glial property. To this end, we histologically observed the structure of the brain via hematoxylin and eosin stain. We also used fluorescent in situ hybridization to evaluate the localization of Msi1, Bmi1, and Sox2. They are essential genes expressed in brain stem cells of loach. Proliferating cells were evaluated by PCNA immunofluorescence. As well as GFAP immunofluorescence was used for detection of the gial property of proliferating cells. Our results indicated that the neural stem cells were located in the granular cell layer of the cerebellum, vagal lobe, facial lobe, optic tectum, and beside the third ventricle because these sites exhibited expression of Sox2. There were several areas of the brain that contained proliferating cells, including the cerebrum, in the torus longitudinalis, and in between the optic tectum and cerebellum. In the cerebellum, the proliferating cells were present in the molecular cell layer, around the intracerebellar ventricle, in the lobus caudalis cerebella, in the eminentia granularis, and around the rhombencephalic ventricle. Proliferating cells were also observed in the three layers of the vagal lobe and throughout the facial lobe. These proliferating cells were all negative for GFAP. The present study provides valuable insight into neural stem cells in loach brain, and these findings can be used to guide future studies.
{"title":"Characterization of neural stem cells and proliferating cells in the brain of loach (Misgurnus anguillicaudatus)","authors":"M. Hussein, Xiaojuan Cao, Yang Li, Mei Jie","doi":"10.21608/EJH.2021.55093.1404","DOIUrl":"https://doi.org/10.21608/EJH.2021.55093.1404","url":null,"abstract":"Loach (Misgurnus anguillicaudatus) is an essential commercial fish in China. We aimed to give some notes on brain histology of loach fish and characterize the sites of stem cells in the brain. As well as, we showed the sites of proliferating cells with detection of their glial property. To this end, we histologically observed the structure of the brain via hematoxylin and eosin stain. We also used fluorescent in situ hybridization to evaluate the localization of Msi1, Bmi1, and Sox2. They are essential genes expressed in brain stem cells of loach. Proliferating cells were evaluated by PCNA immunofluorescence. As well as GFAP immunofluorescence was used for detection of the gial property of proliferating cells. Our results indicated that the neural stem cells were located in the granular cell layer of the cerebellum, vagal lobe, facial lobe, optic tectum, and beside the third ventricle because these sites exhibited expression of Sox2. There were several areas of the brain that contained proliferating cells, including the cerebrum, in the torus longitudinalis, and in between the optic tectum and cerebellum. In the cerebellum, the proliferating cells were present in the molecular cell layer, around the intracerebellar ventricle, in the lobus caudalis cerebella, in the eminentia granularis, and around the rhombencephalic ventricle. Proliferating cells were also observed in the three layers of the vagal lobe and throughout the facial lobe. These proliferating cells were all negative for GFAP. The present study provides valuable insight into neural stem cells in loach brain, and these findings can be used to guide future studies.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"174 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76524360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-25DOI: 10.21608/EJH.2021.55443.1405
A. Sewelam, M. Shehata
Background: Though Nano-zinc oxide particles (ZnO NPs) are widely applied in biomedicine, bioengineering and cosmetology, a controversy developed between ZnO NPs benefits versus toxicity on biological systems. Naringenin is a natural antioxidant flavonoid. Objective: to explore the histological and immunohistochemical alterations in the rat pancreas following intraperitoneal exposure to two different doses of 35 nm ZnO NPs and to assess the ameliorating role of Naringenin.Materials and Methods: Forty-five adult male rats were split randomly into five groups. Group1 served as control. Groups2 and 4 received a single intraperitoneal injection of 250 and 700 mg/kgbw ZnO NPs. Groups3 and 5 administered ZnO NPs as previously described followed by Naringenin gavaged at a dose of 20mg/kgbw/day once daily for 14 consecutive days. Histological, immunohistochemistry, biochemistry, and morphometry studies were accessed in the pancreatic tissue gained from all animals under the study. Results: Contrasted to the group of control, ZnO NPs exhibited a dose dependent pancreatic tissue and cellular damage manifested as vascular congestion, duct dilatation, fibrosis, and inflammatory cell infiltration. Also, both acinar and B-cells showed degenerating changes varied from just cytoplasmic vacuolization in the ZnO NPs(250mg)-treated rats to severe cell shrinking, pyknosis, cytoplasmic and nuclear fragmentation in the ZnO NPs(700mg)-treated rats. Moreover, ZnO NPs provoked significantly increased mean area percentage of collagen fiber deposition and caspase immunoexpressing, significantly raised fasting blood glucose, serum amylase, lipase and MDA levels besides significant decline regarding insulin immunoexpressing. Naringenin administration induced a great recovery concerning the ZnO NPs (250mg)-treated rats but partial recovery regarding the ZnO NPs (700mg)-treated rats. Conclusion: ZnO NPs potentially persuaded an oxidating stress manifest as structural and functional toxicity in the rat pancreas with great reversibility by Naringenin coadministration. A future work concerning ZnO NPs toxicity on vital organs and Naringenin role in opposing this impact is recommended.
{"title":"Nano-Zinc Oxide Induced Pancreatic Toxicity and The Ameliorating Role of Naringenin: Histological and immunohistochemical study","authors":"A. Sewelam, M. Shehata","doi":"10.21608/EJH.2021.55443.1405","DOIUrl":"https://doi.org/10.21608/EJH.2021.55443.1405","url":null,"abstract":"Background: Though Nano-zinc oxide particles (ZnO NPs) are widely applied in biomedicine, bioengineering and cosmetology, a controversy developed between ZnO NPs benefits versus toxicity on biological systems. Naringenin is a natural antioxidant flavonoid. Objective: to explore the histological and immunohistochemical alterations in the rat pancreas following intraperitoneal exposure to two different doses of 35 nm ZnO NPs and to assess the ameliorating role of Naringenin.Materials and Methods: Forty-five adult male rats were split randomly into five groups. Group1 served as control. Groups2 and 4 received a single intraperitoneal injection of 250 and 700 mg/kgbw ZnO NPs. Groups3 and 5 administered ZnO NPs as previously described followed by Naringenin gavaged at a dose of 20mg/kgbw/day once daily for 14 consecutive days. Histological, immunohistochemistry, biochemistry, and morphometry studies were accessed in the pancreatic tissue gained from all animals under the study. Results: Contrasted to the group of control, ZnO NPs exhibited a dose dependent pancreatic tissue and cellular damage manifested as vascular congestion, duct dilatation, fibrosis, and inflammatory cell infiltration. Also, both acinar and B-cells showed degenerating changes varied from just cytoplasmic vacuolization in the ZnO NPs(250mg)-treated rats to severe cell shrinking, pyknosis, cytoplasmic and nuclear fragmentation in the ZnO NPs(700mg)-treated rats. Moreover, ZnO NPs provoked significantly increased mean area percentage of collagen fiber deposition and caspase immunoexpressing, significantly raised fasting blood glucose, serum amylase, lipase and MDA levels besides significant decline regarding insulin immunoexpressing. Naringenin administration induced a great recovery concerning the ZnO NPs (250mg)-treated rats but partial recovery regarding the ZnO NPs (700mg)-treated rats. Conclusion: ZnO NPs potentially persuaded an oxidating stress manifest as structural and functional toxicity in the rat pancreas with great reversibility by Naringenin coadministration. A future work concerning ZnO NPs toxicity on vital organs and Naringenin role in opposing this impact is recommended.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"2 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87599004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-23DOI: 10.21608/EJH.2021.54287.1402
N. Wahba, D. Kashkoush, Rabab Hassan
Background: Mucosal ulcers are common lesions that arise from chemical damage to oral mucosa. Phenytoin is one of the promising wound healing agents that may have the ability to decrease the duration of the healing process of wounds. Objectives: To evaluate the effect of topical application of phenytoin on chemically induced buccal mucosal ulcer of albino rat. Material and methods: 63 male albino rats were subjected to chemical ulcer, then rats were divided into 3 main groups. Group I (control): rats did not receive any treatment, right side buccal mucosa acted as a negative control (Group I -ve), while left side (ulcerated) served as a positive control (Group I +ve). Group II (plain gel) rats received topical application of plain gel twice daily on the ulcer from the day following ulcer induction till scarification. Group III (phenytoin gel) rats received topical application of 1% phenytoin gel twice daily on the ulcer from the day following ulcer induction scarification. Each group was further divided into 3 subgroups A, B and C in which rats were sacrificed at 4, 7 and 12 days following ulcer induction respectively. Buccal mucosae were dissected and examined histologically and immunohistochemically.Results: Histologically, group I+ve showed complete epithelial degeneration at day 4, re-epithelization at day 7, complete regeneration at day 12. Group II showed complete epithelial degeneration day 4, re-epithelization started at day 7, partial regeneration at day 12. Group III showed beginning of re-epithelization at day 4, continuous epithelial lining at day 7, complete regeneration at day 12. Immunohistochemically and statistically, group III showed the highest anti-PCNA expression regarding positive epithelial cells followed by group II then group I+ve. Group I-ve showed the lowest mean. Conclusions: Topical application of phenytoin 1% accelerate healing of chemically induced ulcers through increased vascularization, decreased inflammatory cells and hastened re-epithelization.
{"title":"Histological and Immunohistochemical Study of the Effect of Topically Applied Phenytoin on Chemically Induced Buccal Mucosal Ulcer in Albino Rats","authors":"N. Wahba, D. Kashkoush, Rabab Hassan","doi":"10.21608/EJH.2021.54287.1402","DOIUrl":"https://doi.org/10.21608/EJH.2021.54287.1402","url":null,"abstract":"Background: Mucosal ulcers are common lesions that arise from chemical damage to oral mucosa. Phenytoin is one of the promising wound healing agents that may have the ability to decrease the duration of the healing process of wounds. Objectives: To evaluate the effect of topical application of phenytoin on chemically induced buccal mucosal ulcer of albino rat. Material and methods: 63 male albino rats were subjected to chemical ulcer, then rats were divided into 3 main groups. Group I (control): rats did not receive any treatment, right side buccal mucosa acted as a negative control (Group I -ve), while left side (ulcerated) served as a positive control (Group I +ve). Group II (plain gel) rats received topical application of plain gel twice daily on the ulcer from the day following ulcer induction till scarification. Group III (phenytoin gel) rats received topical application of 1% phenytoin gel twice daily on the ulcer from the day following ulcer induction scarification. Each group was further divided into 3 subgroups A, B and C in which rats were sacrificed at 4, 7 and 12 days following ulcer induction respectively. Buccal mucosae were dissected and examined histologically and immunohistochemically.Results: Histologically, group I+ve showed complete epithelial degeneration at day 4, re-epithelization at day 7, complete regeneration at day 12. Group II showed complete epithelial degeneration day 4, re-epithelization started at day 7, partial regeneration at day 12. Group III showed beginning of re-epithelization at day 4, continuous epithelial lining at day 7, complete regeneration at day 12. Immunohistochemically and statistically, group III showed the highest anti-PCNA expression regarding positive epithelial cells followed by group II then group I+ve. Group I-ve showed the lowest mean. Conclusions: Topical application of phenytoin 1% accelerate healing of chemically induced ulcers through increased vascularization, decreased inflammatory cells and hastened re-epithelization.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"16 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82615037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-19DOI: 10.21608/EJH.2021.49889.1386
E. Laag, Sadika M. Tawfik
Background: Aluminum compounds are commonly used in many human activities. They are used in pesticides, detergents, cosmetics, pharmaceuticals, and food additives. Many studies revealed a link between human exposure to aluminum and many neurodegenerative conditions in which there is platelet dysfunction.Aim of the study: The aim of this research was to study the potential protective effect of platelet rich plasma on the deleterious histological effect of aluminum chloride on male rat cerebellar cortex.Materials and methods: In this study, three equal groups of adult male albino rats were used; each consisted of 10 rats: group I a and I b (control group), group II (daily intraperitoneal injection of aluminum chloride dissolved in saline for 60 consecutive days in a dose of 10mg/kg) and group III were given PRP (subcutaneous injection in a dose of 0.5 ml/kg twice weekly) in addition to aluminum chloride in the same previous dose and duration. Cerebellum sections were processed for hematoxylin and eosin staining, GFAB staining and electron microscopic examination.Results: Aluminum chloride administration resulted in many histological alterations. Glial fibrillary acidic protein-positive cells were significantly present in the cerebellum of aluminum chloride-treated animals relative to control animals. Purkinje cells with darkly stained ill-defined nuclei with dark vacuolated cytoplasm with dilated RER cisterns, swollen mitochondria with destroyed crista were observed in an ultrastructural study for the cerebellar cortex of the aluminum chloride-treated group. It also showed degenerated granule cells with the disappearance of the nuclear membrane and vacuolation of the cytoplasm. Some of the myelinated nerve fibers showed degenerative changes. Concomitant administration of PRP decreased these effects.Conclusion: PRP partially minimized the severity of aluminum chloride-induced cerebellar cortex injurious histological effects in male albino rats. Further research is needed to extend these findings to clinical practice.
{"title":"Histological study of the possible protective action of platelet rich plasma on the injurious effect induced by aluminum chloride on albino male rat cerebellar cortex","authors":"E. Laag, Sadika M. Tawfik","doi":"10.21608/EJH.2021.49889.1386","DOIUrl":"https://doi.org/10.21608/EJH.2021.49889.1386","url":null,"abstract":"Background: Aluminum compounds are commonly used in many human activities. They are used in pesticides, detergents, cosmetics, pharmaceuticals, and food additives. Many studies revealed a link between human exposure to aluminum and many neurodegenerative conditions in which there is platelet dysfunction.Aim of the study: The aim of this research was to study the potential protective effect of platelet rich plasma on the deleterious histological effect of aluminum chloride on male rat cerebellar cortex.Materials and methods: In this study, three equal groups of adult male albino rats were used; each consisted of 10 rats: group I a and I b (control group), group II (daily intraperitoneal injection of aluminum chloride dissolved in saline for 60 consecutive days in a dose of 10mg/kg) and group III were given PRP (subcutaneous injection in a dose of 0.5 ml/kg twice weekly) in addition to aluminum chloride in the same previous dose and duration. Cerebellum sections were processed for hematoxylin and eosin staining, GFAB staining and electron microscopic examination.Results: Aluminum chloride administration resulted in many histological alterations. Glial fibrillary acidic protein-positive cells were significantly present in the cerebellum of aluminum chloride-treated animals relative to control animals. Purkinje cells with darkly stained ill-defined nuclei with dark vacuolated cytoplasm with dilated RER cisterns, swollen mitochondria with destroyed crista were observed in an ultrastructural study for the cerebellar cortex of the aluminum chloride-treated group. It also showed degenerated granule cells with the disappearance of the nuclear membrane and vacuolation of the cytoplasm. Some of the myelinated nerve fibers showed degenerative changes. Concomitant administration of PRP decreased these effects.Conclusion: PRP partially minimized the severity of aluminum chloride-induced cerebellar cortex injurious histological effects in male albino rats. Further research is needed to extend these findings to clinical practice.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"22 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72836568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-19DOI: 10.21608/EJH.2021.55723.1408
Soad Shebl Shebl, R. Yassien, Shaimaa Abou-Ghanima, S. El-Nabi, Eman S El-Roghy
AbstractBackground: Diabetes mellitus is a worsening worldwide health problem. It constitutes a major global health concern. Sitagliptin selectively inhibits dipeptidyl peptidase-4 (DPP-4) to manage type 2 diabetes mellitus (T2DM). It regulates the blood glucose level without risk of hypoglycemia or increase in body weight. In our study we investigated the effects of Sitagliptin on DNA and chromosomes in cultured human lymphocytes. Aim: To assess the genotoxic and cytotoxic effects of different concentrations of Sitagliptin on cultured human lymphocytes.Material and methods: Cultures were divided into 6 groups: control, positive control (Cisplatin) at concentration of 10 μg/mL and 4 different concentrations of Sitagliptin (125,250,500,1000 μg/mL). Sitagliptin genotoxicity and cytotoxicity were determined by using chromosomal aberrations (CAs), mitotic index (MI), comet assay and nucleic acids electrophoresis.Results: There was high significant increase in total chromosomal aberrations (TCAs) at 500, 1000 μg/mL of Sitagliptin compared to control. Other studied concentrations of Sitagliptin exhibited an increase in TCAs without significant relation. Compared to control, there was a significant increase in mitotic index at 125 μg/mL of Sitagliptin but non-significant increase at 250 μg/mL of Sitagliptin. However, at 500, 1000 μg/mL of Sitagliptin, there was a significant decrease in MI. Regarding comet assay, there was significant and high significant increase in total DNA damage at 500,1000 μg/mL of Sitagliptin respectively. Nucleic acids electrophoresis not digested with RNase showed that optical density value of RNA was maximum at 125 μg/mL then gradually decreased till reach the minimum level at 1000 μg/mL of Sitagliptin indicating its toxicity. Genomic DNA fragmentation results indicated that Sitagliptin caused a slight damage of DNA in the form of necrosis in a concentration dependent manner. Conclusion: Sitagliptin induces significant genotoxic and cytotoxic effects on the cultured human lymphocytes at concentrations of (500, 1000 μg/mL).
{"title":"Genetic Study on the Effect of the Antidiabetic Drug ( Sitagliptin) on DNA and Chromosomes of Human Lymphocyte Culture","authors":"Soad Shebl Shebl, R. Yassien, Shaimaa Abou-Ghanima, S. El-Nabi, Eman S El-Roghy","doi":"10.21608/EJH.2021.55723.1408","DOIUrl":"https://doi.org/10.21608/EJH.2021.55723.1408","url":null,"abstract":"AbstractBackground: Diabetes mellitus is a worsening worldwide health problem. It constitutes a major global health concern. Sitagliptin selectively inhibits dipeptidyl peptidase-4 (DPP-4) to manage type 2 diabetes mellitus (T2DM). It regulates the blood glucose level without risk of hypoglycemia or increase in body weight. In our study we investigated the effects of Sitagliptin on DNA and chromosomes in cultured human lymphocytes. Aim: To assess the genotoxic and cytotoxic effects of different concentrations of Sitagliptin on cultured human lymphocytes.Material and methods: Cultures were divided into 6 groups: control, positive control (Cisplatin) at concentration of 10 μg/mL and 4 different concentrations of Sitagliptin (125,250,500,1000 μg/mL). Sitagliptin genotoxicity and cytotoxicity were determined by using chromosomal aberrations (CAs), mitotic index (MI), comet assay and nucleic acids electrophoresis.Results: There was high significant increase in total chromosomal aberrations (TCAs) at 500, 1000 μg/mL of Sitagliptin compared to control. Other studied concentrations of Sitagliptin exhibited an increase in TCAs without significant relation. Compared to control, there was a significant increase in mitotic index at 125 μg/mL of Sitagliptin but non-significant increase at 250 μg/mL of Sitagliptin. However, at 500, 1000 μg/mL of Sitagliptin, there was a significant decrease in MI. Regarding comet assay, there was significant and high significant increase in total DNA damage at 500,1000 μg/mL of Sitagliptin respectively. Nucleic acids electrophoresis not digested with RNase showed that optical density value of RNA was maximum at 125 μg/mL then gradually decreased till reach the minimum level at 1000 μg/mL of Sitagliptin indicating its toxicity. Genomic DNA fragmentation results indicated that Sitagliptin caused a slight damage of DNA in the form of necrosis in a concentration dependent manner. Conclusion: Sitagliptin induces significant genotoxic and cytotoxic effects on the cultured human lymphocytes at concentrations of (500, 1000 μg/mL).","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"20 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88257584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-07DOI: 10.21608/EJH.2021.46953.1377
shimaa Antar, S. Hassan
Introduction: excessive use of paracetamol with caffeine in Panadol extra during pregnancy increase the risk of spontaneous abortion. Vitamin D stimulates cardiomyocyte proliferation and protects against injury. Aim of Work: To investigate the effect of maternal exposure to large doses of Panadol extra on the development of the embryonic hearts of rat’s offspring and possible protective effects of vitamin D.Material and Methods: Forty-five albino rats; 30 adult females and 15 adult males were used in this study. The pregnant rats were divided into Group I as a control group, Group II (treated group; Panadol extra) received the maximum daily dose (4000mg paracetamol/ 520 mg caffeine) / kg/ day and Group III (protective group; Panadol extra + vitamin D) received previously mentioned dose of Panadol extra plus vitamin D at a dose of 400 IU/kg/day. All the treatments were given by oral gavage from the 1st day of pregnancy until day 19. Pregnant rats from all groups were sacrificed on day 19 of pregnancy and their pups were extracted & weighted, then sacrificed. Blood samples were taken for biochemical assessment. Sections from the hearts were stained (H&E, Eosin & Mallory’s trichrome stain), Immunohistochemical (alpha-smooth muscles) for histopathological and morphometric studies.Results: group (II) revealed changes of blood gases as PH, increase in PCO2, decrease PO2, high lipid profile, and increase Creatinine kinase-MB. There were different histopathological changes as cardiomyocyte damage, necrosis, and inflammation with increased fibrosis by Mallory stain. The morphometric results revealed an enlarged size of cardiac cells, decreased proliferation, and maturation. All these results were greatly improved in the group (III) when concomitant administration of vitamin D in the last protective group. Conclusion: prolonged and excessive maternal use of Panadol extra greatly affect heart development. Vitamin D has a potential effect on cardiac regeneration and development.
简介:妊娠期间过量使用扑热息痛与Panadol extra中的咖啡因会增加自然流产的风险。维生素D刺激心肌细胞增殖，防止损伤。工作目的:探讨母体大剂量帕纳多对大鼠子代胚胎心脏发育的影响及维生素d可能的保护作用。研究对象为30名成年女性和15名成年男性。妊娠大鼠分为ⅰ组为对照组，ⅱ组(治疗组;Panadol extra)接受最大日剂量(4000mg扑热息痛/ 520 mg咖啡因)/ kg/天和第三组(保护组;Panadol extra +维生素D)接受了之前提到的Panadol extra +维生素D的剂量，剂量为400 IU/kg/天。从妊娠第1天至第19天均采用灌胃治疗。各组孕鼠于妊娠第19天处死，取幼鼠称重后处死。采集血样进行生化评估。心脏切片染色(H&E, Eosin & Mallory’s三色染色)，免疫组化(α -平滑肌)进行组织病理学和形态计量学研究。结果:II组血气PH值改变，PCO2升高，PO2降低，血脂升高，肌酐激酶mb升高。Mallory染色显示心肌细胞损伤、坏死、炎症及纤维化增加。形态学结果显示心肌细胞增大，增殖减少，成熟。在最后一个保护组同时给予维生素D时，这些结果在第三组有很大的改善。结论:产妇长期过量使用帕纳多对心脏发育影响较大。维生素D对心脏再生和发育有潜在影响。
{"title":"Effect of Maternal Panadol Extra Exposure on Embryonic Heart of Rat’s Offspring and Possible Protective Effect of Vitamin D","authors":"shimaa Antar, S. Hassan","doi":"10.21608/EJH.2021.46953.1377","DOIUrl":"https://doi.org/10.21608/EJH.2021.46953.1377","url":null,"abstract":"Introduction: excessive use of paracetamol with caffeine in Panadol extra during pregnancy increase the risk of spontaneous abortion. Vitamin D stimulates cardiomyocyte proliferation and protects against injury. Aim of Work: To investigate the effect of maternal exposure to large doses of Panadol extra on the development of the embryonic hearts of rat’s offspring and possible protective effects of vitamin D.Material and Methods: Forty-five albino rats; 30 adult females and 15 adult males were used in this study. The pregnant rats were divided into Group I as a control group, Group II (treated group; Panadol extra) received the maximum daily dose (4000mg paracetamol/ 520 mg caffeine) / kg/ day and Group III (protective group; Panadol extra + vitamin D) received previously mentioned dose of Panadol extra plus vitamin D at a dose of 400 IU/kg/day. All the treatments were given by oral gavage from the 1st day of pregnancy until day 19. Pregnant rats from all groups were sacrificed on day 19 of pregnancy and their pups were extracted & weighted, then sacrificed. Blood samples were taken for biochemical assessment. Sections from the hearts were stained (H&E, Eosin & Mallory’s trichrome stain), Immunohistochemical (alpha-smooth muscles) for histopathological and morphometric studies.Results: group (II) revealed changes of blood gases as PH, increase in PCO2, decrease PO2, high lipid profile, and increase Creatinine kinase-MB. There were different histopathological changes as cardiomyocyte damage, necrosis, and inflammation with increased fibrosis by Mallory stain. The morphometric results revealed an enlarged size of cardiac cells, decreased proliferation, and maturation. All these results were greatly improved in the group (III) when concomitant administration of vitamin D in the last protective group. Conclusion: prolonged and excessive maternal use of Panadol extra greatly affect heart development. Vitamin D has a potential effect on cardiac regeneration and development.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"3 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76934769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-05DOI: 10.21608/EJH.2021.51729.1392
D. El-ghazouly, R. Yassien
Background: Sofosbuvir (SOF) is a hopeful remedy for chronic hepatitis C virus infection. However, it records some unfavorable influences on gastrointestinal tract. Fucoidan is a sulfated polysaccharide having cytoprotective effect besides anti-inflammatory and antioxidant properties.Aim: This work has been focused on the possible effect of sofosbuvir on the fundic mucosa and to assess the probable role of protection of fucoidan.Materials and methods: we divided 40 adult male albino rats equally into four groups and were received drugs as a single daily dose for five weeks. Control group (I): were received distilled water and normal saline. Fucoidan group (II): were received 80 mg/kg. Sofosbuvir group (III): were received 4 mg/kg. Group IV: were received Fucoidan and Sofosbuvir with the same doses as pervious groups. All rats were sacrificed one day following the last dose. Stomach specimens were obtained and prepared for light and electron microscopic examination.Results: Sofosbuvir-treated rats showed dilated fundic pits and cystic dilatation of fundic glands with flattening of their epithelial lining. Vacuolation of cytoplasm and shrunken pyknotic nuclei were detected in parietal cells. Dilatation of rough endoplasmic reticulum and loss of secretory granules were detected in the chief cells. Infiltration of inflammatory cells with congested blood vessels and extravasated RBCs were detected in lamina propria. Statistically, there was a highly significant rise in the area% of collagen fibers while area% of Periodic Acid Schieff -Alcian Blue stain (PAS–AB) reaction showed a highly significant reduction comparing with the control. The immunohistochemical results revealed marked increase in proliferating cell nuclear antigen (PCNA) expressions. The administration of Fucoidan with Sofosbuvir minimized these changes.Conclusion: Sofosbuvir has been proved to induce histological changes in the fundic mucosa and these changes can be attenuated by Fucoidan when given in concomitant with it.
{"title":"Effect of Sofosbuvir (Sovaldi) on the fundic mucosa of adult male albino rats and the possible protective role of Fucoidan: Histological, histochemical, and immunohistochemical study","authors":"D. El-ghazouly, R. Yassien","doi":"10.21608/EJH.2021.51729.1392","DOIUrl":"https://doi.org/10.21608/EJH.2021.51729.1392","url":null,"abstract":"Background: Sofosbuvir (SOF) is a hopeful remedy for chronic hepatitis C virus infection. However, it records some unfavorable influences on gastrointestinal tract. Fucoidan is a sulfated polysaccharide having cytoprotective effect besides anti-inflammatory and antioxidant properties.Aim: This work has been focused on the possible effect of sofosbuvir on the fundic mucosa and to assess the probable role of protection of fucoidan.Materials and methods: we divided 40 adult male albino rats equally into four groups and were received drugs as a single daily dose for five weeks. Control group (I): were received distilled water and normal saline. Fucoidan group (II): were received 80 mg/kg. Sofosbuvir group (III): were received 4 mg/kg. Group IV: were received Fucoidan and Sofosbuvir with the same doses as pervious groups. All rats were sacrificed one day following the last dose. Stomach specimens were obtained and prepared for light and electron microscopic examination.Results: Sofosbuvir-treated rats showed dilated fundic pits and cystic dilatation of fundic glands with flattening of their epithelial lining. Vacuolation of cytoplasm and shrunken pyknotic nuclei were detected in parietal cells. Dilatation of rough endoplasmic reticulum and loss of secretory granules were detected in the chief cells. Infiltration of inflammatory cells with congested blood vessels and extravasated RBCs were detected in lamina propria. Statistically, there was a highly significant rise in the area% of collagen fibers while area% of Periodic Acid Schieff -Alcian Blue stain (PAS–AB) reaction showed a highly significant reduction comparing with the control. The immunohistochemical results revealed marked increase in proliferating cell nuclear antigen (PCNA) expressions. The administration of Fucoidan with Sofosbuvir minimized these changes.Conclusion: Sofosbuvir has been proved to induce histological changes in the fundic mucosa and these changes can be attenuated by Fucoidan when given in concomitant with it.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"10 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88522846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-31DOI: 10.21608/ejh.2020.53466.1401
N. Bahey, Noha Elswaidy
Introduction: Potassium bromate (KBrO3) is an oxidizing agent that is widely used as a flour improver. However, it induces genotoxic and carcinogenic effects on different body organs in a dose and duration-dependent manner. So, the aim of this study was: to explore the effects of KBrO3 on the structure of the rat jejunal mucosa and investigate the potential role of lycopene, a strong antioxidant molecule, in preventing or ameliorating the effect of KBrO3. Materials & Methods: Twenty-four adult male albino rats were used and divided equally into four experimental groups; control group, lycopene group that received 10mg of lycopene/kg/day/orally; KBrO3 group that received 100mg of KBrO3/kg/day/ orally and KBrO3 and lycopene group that received KBrO3 and lycopene in the same doses as in the previous groups. Animals were sacrificed after 4 weeks and the specimens from the jejunum were processed for histological, immunohistochemical, and ultrastructural examinations. Results: the jejunal mucosa of the KBrO3 treated group showed short and broad villi, discontinuity and desquamation of their lining epithelial cells, inflammatory cellular infiltration, and dilatation of the blood vessels. Moreover, there was a significant decrease in the number of goblet cells and PCNA immuno-stained nuclei in the jejunum. Ultramicroscopic examination showed swollen vacuolated mitochondria, dilated rough endoplasmic reticulum, and shrunken dark nuclei. Interestingly, the group treated with both lycopene and KBrO3 showed less cytoplasmic vacuolation and mitochondrial abnormalities in the epithelial cells lining the villi. Furthermore, there was a significant improvement in the height of jejunal villi, the number of goblet cells, and PCNA immuno-stained nuclei. In conclusion: KBrO3 induced cellular damage in the rat jejunal mucosa which was prevented by coadministration of lycopene.
{"title":"Lycopene reduces potassium bromate-induced structural alterations in the jejunal mucosa of adult rats","authors":"N. Bahey, Noha Elswaidy","doi":"10.21608/ejh.2020.53466.1401","DOIUrl":"https://doi.org/10.21608/ejh.2020.53466.1401","url":null,"abstract":"Introduction: Potassium bromate (KBrO3) is an oxidizing agent that is widely used as a flour improver. However, it induces genotoxic and carcinogenic effects on different body organs in a dose and duration-dependent manner. So, the aim of this study was: to explore the effects of KBrO3 on the structure of the rat jejunal mucosa and investigate the potential role of lycopene, a strong antioxidant molecule, in preventing or ameliorating the effect of KBrO3. Materials & Methods: Twenty-four adult male albino rats were used and divided equally into four experimental groups; control group, lycopene group that received 10mg of lycopene/kg/day/orally; KBrO3 group that received 100mg of KBrO3/kg/day/ orally and KBrO3 and lycopene group that received KBrO3 and lycopene in the same doses as in the previous groups. Animals were sacrificed after 4 weeks and the specimens from the jejunum were processed for histological, immunohistochemical, and ultrastructural examinations. Results: the jejunal mucosa of the KBrO3 treated group showed short and broad villi, discontinuity and desquamation of their lining epithelial cells, inflammatory cellular infiltration, and dilatation of the blood vessels. Moreover, there was a significant decrease in the number of goblet cells and PCNA immuno-stained nuclei in the jejunum. Ultramicroscopic examination showed swollen vacuolated mitochondria, dilated rough endoplasmic reticulum, and shrunken dark nuclei. Interestingly, the group treated with both lycopene and KBrO3 showed less cytoplasmic vacuolation and mitochondrial abnormalities in the epithelial cells lining the villi. Furthermore, there was a significant improvement in the height of jejunal villi, the number of goblet cells, and PCNA immuno-stained nuclei. In conclusion: KBrO3 induced cellular damage in the rat jejunal mucosa which was prevented by coadministration of lycopene.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"IM-36 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84774525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：