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ISOLATION AND IDENTIFICATION OF ROTAVIRUS FROM NEONATAL CALVES 新生牛犊轮状病毒的分离与鉴定
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1341
Shoney M. Odisho, A. Albana, Amjad KH. Majeed
 Bovine rotavirus was successfully isolated in vero cell line and secondary calf kidney cell culture from faecal samples of newly bom calves affected with diarrhoea. The isolated viruses were cultivated from cell culture produced cytopathogenic effect which consisted of cytoplasmic granulation degeneration and detachment of infected cells from monolayer cell culture. The rotavirus was identified by indirect immunofluorecent test (IFT) and neutralization test (NT) by using reference hyperimmunser.
从患腹泻的新生牛犊粪便样本中成功分离出牛轮状病毒。从细胞培养中分离出的病毒产生细胞致病作用,主要表现为细胞质肉芽变性和感染细胞脱离单层细胞培养。采用间接免疫荧光试验(IFT)和参比超免疫剂中和试验(NT)对轮状病毒进行鉴定。
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引用次数: 0
COMPARISON OF THREE METHODS FOR THE DETECTION OF ROTAVIRUS ANTIGEN IN THE FAECES OF NEW BORN CALVES 新生儿粪便中轮状病毒抗原三种检测方法的比较
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1356
Shoney M. Odisho, Nidhal A. Muhamad, Antons S. Al- Bana
    Immunofluorescence on incoulated cell cultures, latex agglutination test and enzyme-linked immunosorbant assay (ELISA) were compared for the detection of rotavirus antigen in faecal sample from new born calves. Rotavirus were detected in 20 faecal sample by immunfluorescence test, latex agglutination test demonstrated rotavirus in 27 faecal sample, and rotavirus were detected in 29 out of 82 faecal sample by ELISA.
采用细胞培养免疫荧光法、乳胶凝集试验和酶联免疫吸附试验(ELISA)检测新生犊牛粪便中轮状病毒抗原。免疫荧光法检测到轮状病毒20例,乳胶凝集法检测到轮状病毒27例,ELISA法检测到轮状病毒29例。
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引用次数: 0
PREVALENCE OF GASTROPHILUS LARVAE IN DONKEYS 驴胃门幼虫的患病率
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1339
Sami R. ALI, Rafaa Y. AL- KUBAISEE, Raad H. RAHIF
The stomach, small and large intestine of 140 donkeys were examined for Gastrophilus larvae. The rate of infection was 45%, 6.4% and 7.8% for Gastrophilus intestinalis, G. nasalis G. pecorum, respectively, with the mean larval counts of 3rd instars; 25.8, 10.4 and 27.7, for the respective species. Excepting the month October, third instars of G. intestinalis were found throughout the year. The peak of infection (87.5%) was recorded in February, while the lowest rate (7.1%) was in November. This rate and mean larval burden seemed to decline with the age of the host. The sex of the animals had no effect on the rate of infection. G. pecorum, and G. nasalis are reported for the first time in Iraq and occurred only during the months of June, August and September.
对140头驴的胃、小肠和大肠进行了嗜胃菌幼虫的检查。肠胃杆菌和鼻胃杆菌的感染率分别为45%、6.4%和7.8%,平均幼虫数为3龄;25.8、10.4和27.7。除10月份外,全年都有三龄肠杆菌出现。感染高峰(87.5%)出现在2月份,而感染率最低(7.1%)发生在11月份。这一比率和平均幼虫负荷似乎随着宿主年龄的增长而下降。动物的性别对感染率没有影响。G.pecorum和G.nasalis在伊拉克首次报道,仅发生在6月、8月和9月。
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引用次数: 0
AN ELECTROPHORETIC STUDY ON SERUM PROTEINS IN NORMAL ARABIAN RACE HORSES 正常阿拉伯赛马血清蛋白的电泳研究
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1364
A. Sultan, A.K.A AL-Delaimi
Electrophoretic Pattern of Serum proteins were studied in thirty normal Arabian race horses. The resolution of the serum protein fractions in 24 (80%) of horses, group I revealed five fractions, albumin and the globulins (a1, a2, B and y). The total serum protein was ranged from ( 6.2-8.4 gm/dL) with mean (7.57 ±0.17) in the this group of horses.  Where-as four fractions, albumin and the globulins (a2, B and y) were observed in 6(20%) of horses, group 2. The total serum protein was ranged from (6.0 - 7.4 gm/dL) with mean (6.76 ± 0.27) in that group of horses.  The Albumin/Globulin ratio in both Groups was (0.66 ±0.13); (0.89 ± 0.07), respectively.
对30匹正常阿拉伯赛马的血清蛋白电泳图谱进行了研究。第一组24匹马(80%)的血清蛋白质组分的分辨率显示了五个组分,白蛋白和球蛋白(a1、a2、B和y)。这组马的总血清蛋白范围为(6.2-8.4 gm/dL),平均值为(7.57±0.17)。其中,作为四个组分,在第2组的6匹马(20%)中观察到白蛋白和球蛋白(a2、B和y)。该组马的总血清蛋白范围为(6.0-7.4 gm/dL),平均值为(6.76±0.27)。两组白蛋白/球蛋白比值均为(0.66±0.13);(0.89±0.07)。
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引用次数: 0
THE USE OF OXYTOCIN IN COMBINATION WITH UTERINE LAVAGE AS A POST-MATING TREATMENT FOR INFERTILE MARES WITH ENDOMETRITIS 催产素联合子宫灌洗治疗不孕母马子宫内膜炎
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1360
Abdul-Aziz F.R.
   Eighteen infertile multiparous mares were used in this study. They had been barren for at least 2 years and had been bred repeatedly during those years, and were 8-20 years of age. Clinical genital, and endometrial cytology and histopathological examinations revealed that the mares suffered from acute endometritis. Each mare was subjected to an intrauterine lavage with 500-1000 ml of sterile saline solution in combination with an intravenous injection of 20 I.U. of oxytocin within 12-24 hours after mating. The mares expelled most of the flushed fluid within 20 -30 minutes of oxytocin injection. Eighteen of the treated mares, 13 (72%) conceived after mating on one estrous period. Results of this study indicates that removing intrauterine fluid containing inflammatory by-products by saline lavage and inducing uterine contractions with oxytocin after mating improved conception rates in infertile mares.
本研究使用了18匹不育的多胎母马。它们已经贫瘠了至少2年,在这些年里反复繁殖,年龄在8-20岁之间。临床生殖器、子宫内膜细胞学和组织病理学检查显示母马患有急性子宫内膜炎。每匹母马在交配后12-24小时内用500-1000毫升无菌生理盐水进行宫内灌洗,并静脉注射20 I.U.的催产素。母马在注射催产素后20-30分钟内排出了大部分冲洗液。18只接受治疗的母马,13只(72%)在一次发情期交配后受孕。这项研究的结果表明,通过盐水灌洗去除含有炎症副产物的宫内液体,并在交配后用催产素诱导子宫收缩,可以提高不育母马的受孕率。
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引用次数: 0
HISTOPATHOLOGICAL AND HISTOCHEMICAL CHANGES IN THE IUNG OF GUINEA PIGS EXPERIMENTALLY INFECTED WITH NOCARDIA ASTEROIDES ' 实验性小行星诺卡菌感染豚鼠肺部组织病理学和组织化学变化
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1367
M. J. Alwan, B. I. Alkaisie, R. Abdul Rahman
The pathological and histochemical changes inthe lung of ten guinea pigs have been investigated after using viable Nocardia asteroides in a dose of 1.5*108 cfc, thebacteria was injected intrapulmonary. Lesions of pyogranuloma were developed. Histochemical studies showed that the granules of goblet cells and mucos glands of lung contain a mixture of neutral and sialomucin normal animal , these types of mucin changed to sialomucin and sulfomucin inthe infected animals.
研究了10只豚鼠肺内注射活诺卡菌(1.5*108 cfc)后肺内病理和组织化学变化。出现脓肉芽肿病变。组织化学研究表明,正常动物的杯状细胞颗粒和肺粘膜腺中含有中性黏液蛋白和唾液黏液蛋白的混合物,感染动物的这种黏液蛋白转变为唾液黏液蛋白和硫黏液蛋白。
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引用次数: 0
SURVIVAL OF DIFFERENT BACTERIAL SPECIES ON CEMENT AND PORCELAIN SURFACES. AT LOW TEMPERATURE. 不同菌种在水泥和瓷器表面的存活。在低温下。
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1355
A. A.Razzak
Fourteen different bacterial species were used in a study of their abilities to survive on cement and porcelain surfaces. A dose of 5x10 cfu/drop (50 ul) for each species was used to contaminate each surface. The bacteria used included: Bacillus subtilis, Brucella abortus, Coraynebacterium pyogenes, E.coli, Klebsiella pnemmoniae, Listeria monocytogenes, Pasteurella multocida, Proteus vulgaris, Pseudomonas aergenosa, Salmonella typhi, Staphylococus aureus, Staph. Epidermidis, Streptococcus equi & Strept. Zooepidemicus. After 7, 14 and 21 days of storage at 4°C, subsamples were cultured onto different types of media, and after 24-48 hr. of incubation at 37°C, growth or no growth were scored for each type of bacteria used.  Results showed that B. subtilis, Staph.epidermidis & Strept. zooepdemicus were recovered from all surfaces up till 21-day storage. E. coli, Pr.vulgaris, Staph. aureus & Strept.equi were recovered only from Porcelaim surfaces throughout the whole experiment, whereas Past. multocida was recovered at all times from cement surfaces only. Br. abortus was recovered only from cement surfaces after 7 & 14 days of storage, but was never isolated from contaminated Porcelain surfaces. Kl. pneumoniae was isolated from Porcelain surfaces at all times, but only after 7-day- storage from cement surfaces. On the other hand, L. monocytogenes was never recovered from any surface at any time of storage.
研究人员使用了14种不同的细菌来研究它们在水泥和瓷器表面的生存能力。每个物种使用5 × 10 cfu/滴(50 ul)的剂量来污染每个表面。所用细菌包括:枯草芽孢杆菌、流产布鲁氏菌、化脓性冠状杆菌、大肠杆菌、肺炎克雷伯菌、单核增生李斯特菌、多杀性巴氏杆菌、普通变形杆菌、绿脓杆菌、伤寒沙门氏菌、金黄色葡萄球菌、葡萄球菌。表皮病、马链球菌及链球菌。Zooepidemicus。在4°C下保存7、14和21天后,将亚样本培养到不同类型的培养基上,并在24-48小时后进行培养。在37°C的孵育下,对每种细菌的生长或不生长进行评分。结果表明:枯草芽孢杆菌、葡萄球菌;表皮及链球菌。从所有表面回收动物流行病,直到21天的储存。大肠杆菌,寻常原菌,葡萄球菌。金黄色葡萄球菌;在整个实验过程中,马只从瓷表面回收,而过去。在任何时候都只从水泥表面回收多杀菌。Br。保存7天和14天后,流产菌仅从水泥表面回收,但从未从被污染的瓷器表面分离。在任何时候都可以从瓷表面分离到肺炎链球菌,但只有在水泥表面保存7天后才可以分离到肺炎链球菌。另一方面,单核增生乳杆菌在任何储存时间内都无法从任何表面回收。
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引用次数: 0
THE EFFECT OF FEEDING LEVEL ON THE GENETIC RESISTANCE OF LOCAL BREEDS INFECTED WITH HAEMONCHUS CONTORTUS 饲养水平对扭曲血蜱地方品种遗传抗性的影响
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1366
D. A. Ali, M. Al-zuhairy, A. M. Affat
Sixty sheep of three local breeds (Awassi, Hamdani, Karadi) of age, 6-8 months were divided to two groups, the first fed on low level of nutrition. Each group then divided into Two subgroups, the first was infected with 500 larvae / Kg Haemonchus contortus & the second was used as a control. Egg G. fecal sample, staple length, P. c. v. Hb; weight gain were recorded weekly for 13 week post infection. Results revealed that all sheep traits of low level nutrition were significantly (P<0.01) more affected than high level nutrition group. Awassi breed was more reistant to infection with H. contortus than Hamdani & Karadi breeds.
将三个地方品种(Awassi、Hamdani、Karadi)的60只6-8个月龄的绵羊分为两组,第一组喂食低营养水平的绵羊。然后,每组分为两个亚组,第一个亚组感染500只幼虫/Kg扭曲血蜱,第二个亚组作为对照。蛋G.粪便样本,主要长度,P.c.与Hb的比值;感染后13周每周记录体重增加。结果表明,低营养组对绵羊各项性状的影响均显著高于高营养组(P<0.01)。Awassi品种比Hamdani和Karadi品种更容易感染扭曲线虫。
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引用次数: 1
ISOLATION AND PURIFICATION OF AVIDIN FROM EGG WHITE BY IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY 固定化金属亲和色谱法分离纯化蛋清中的抗生物素
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1359
Shalan Alwan Al-Mashikhi
Lysozyme and avidin were initially separated from egg white by cation exchange chromatography (Doulite C-464). Avidin was then isolated from lysozyme in pure form by immobilized metal affinity chromatography column (1.5 x 10cm) loaded with copper ions. The column was equilibrated with 0.02M phosphate buffer pH 7.7, containing 0.5M NaCl. Protein fraction obtained from Doulite column was applied on IMAC column, followed by washing with the starting buffer and eluting with pH gradient with 0.1M Acetic acid. Two peaks were obtained, the first peak represents the avidin, while the second peak represents the lysozyme as tested by SDS-PAGA and HABA assay. The purity of avidin was increased to 75% by the IMAC process.
溶菌酶和抗生物素最初通过阳离子交换色谱法(Doulite C-464)从蛋清中分离。然后通过负载铜离子的固定化金属亲和色谱柱(1.5 x 10cm)从溶菌酶中以纯形式分离阿维丁。用含有0.5M NaCl的0.02M磷酸盐缓冲液(pH 7.7)平衡柱。将从Doulite柱获得的蛋白质级分应用于IMAC柱上,然后用起始缓冲液洗涤并用0.1M乙酸以pH梯度洗脱。获得两个峰,第一个峰代表抗生物素,而第二个峰代表通过SDS-PAGA和HABA测定测试的溶菌酶。通过IMAC工艺,阿维丁的纯度提高到75%。
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引用次数: 0
BOVINE DYSTOCIA IN IRAQ TYPES AND TREATMENTS 伊拉克牛难产的类型和治疗
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1362
D. J. Khammas, T. M. AL-Hamedawi
   Study was conducted on 149 case of cross breed bovine dystocia referred to Department of Theriogenology, College of Veterinary Medicine and Al-Wihda Cattle station, Baghdad, Iraq. Results showed that 87.5% of bovine dystocia were successfully relieved by cesarean section (C.S), 85.5% success was achieved by correction an traction, 71.4% by fetotomy and 50% by hormonal treatment. Most of the cases subjected to C.S, were heifers. Details concerning number of cases subjected to C.S, indications, positive response, state of pregnancy, number of claves recovered, number of male/female and survival rate of claves were listed in two enclosed tables. From results we may conclude that C.S was the best indicative treatment of dystocia, particularly when all the trails and efforts of treatments failed, inspite of the reasonable results obtained from various treatments mentioned in other cases
对伊拉克巴格达兽医学院兽医学科和Al-Wihda牛场收治的149例杂交牛难产病例进行了研究。结果:剖宫产术的牛难产成功率为87.5%,矫正牵引术的成功率为85.5%,胎儿切开术的成功率为71.4%,激素治疗的成功率为50%。大多数感染cs的病例是小母牛。关于cs的病例数、适应症、阳性反应、妊娠状态、恢复的胎数、男女胎数和存活率的详细信息列在两个随附的表格中。从结果中我们可以得出结论,cs是难产的最佳指示性治疗方法,特别是当所有的治疗方法和努力都失败时,尽管其他病例中提到的各种治疗方法都获得了合理的结果
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引用次数: 0
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The Iraqi Journal of Veterinary Medicine
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