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STUDIES ON BOVINE TRAUMATIC RETICULO- PERITONITIS IN BAGHDAD 巴格达牛创伤性网状腹膜炎的研究
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1361
A. A. YASS, I. Q. HASSAN, A. SH. SULTAN
A total of fourty-five cases of traumatic- reticulo- peritonitis in cows (16 cows ill for 24-48 hrs and 29 cows ill for 72-120 hrs). in Baghdad were diagnosed clinically, laboratory and autopical findings. The most valuable diagnostic signs were increased rectal temperature (39.9 C), respiratory rates (36/min.) and heart beats (95/min.), decreased in milk production (100.00 %), ruminal atony (95.56%), abdominal pain (93.33%), disturbed appetite (91.11%), decrease manure production (84.44%) and recurrent tympany (84. 44%). The total and differential leucocyte counts provided good diagnostic and prognostic data. The determination of total plasma protein and fibrinogen were used as aids to the diagnosis of traumatic reticulo-peritonitis in this study.
共有45例奶牛创伤性网织性腹膜炎(16头奶牛患病24-48小时,29头奶牛患病72-120小时)。在巴格达被诊断为临床、实验室和尸检结果。最有价值的诊断体征是直肠温度升高(39.9℃)、呼吸频率(36/min)和心跳(95/min)、产奶量下降(100.00%)、瘤胃无力(95.56%)、腹痛(93.33%)、食欲紊乱(91.11%)、粪便产量下降(84.44%)和复发性鼓室炎(84。44%)。白细胞总数和差异计数提供了良好的诊断和预后数据。血浆总蛋白和纤维蛋白原的测定有助于外伤性网织性腹膜炎的诊断。
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引用次数: 0
The effects of Ivomec on experimental infection of Awasi lambs by H. contortus Ivomec对阿瓦西羔羊弯曲弧菌实验感染的影响
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1369
وفاء حسن عيسى
The effect of Ivomec was studied against stomach large worm H. contortus in experimental Awasi lambs through infection of 500 infective larvac per Kg body weight. Result revealed that Ivomec has high efficacy reaching 100%. Two weeks post treatment, improvment of blood parameter was recorded 3 weeks post treatment indicating its effectivness, there was also decrease in the number of eggs per gram which vanished 2 weeks post treatment. This indicated that Ivomec with a fixed dose has high efficacy against H. contortus the most spread helminth in Iraq
采用每Kg体重500只感染幼虫的方法,研究了Ivomec对实验阿瓦西羔羊胃大虫(H. contortus)的防治效果。结果显示,依维美克的有效率高达100%。治疗后2周,血液指标改善,治疗后3周有效,每克卵数减少,治疗后2周消失。这表明,固定剂量Ivomec对伊拉克最广泛传播的蠕虫H. tortortus有很高的疗效
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引用次数: 0
USE OF SOME MEDICAMENTS IN THE TREATMENT OF AFFECTED CALVES WITH HYPOMAGNESAEMIC TETANY 使用一些药物治疗影响小牛与低镁性手足搐搦症
Pub Date : 2022-11-21 DOI: 10.30539/ijvm.v20i2.1368
W. A. RAZZAK AL-AZZAWI
This trial comprised (28) naturally affected calves with hypomagnesaemic tetany, which were diagnosed clinically and by measuring the serum level of magnesium, calcium, sodium, inorganic phosphorus, potassium and SGOT & CPK enzymes. There were significant decrease (P<0.01) in serum level of magnesium and calcium. While serum potassium and serum enzymes level shown significant increase (P < 0.01) during tetanic attacks. On the other hand serum sodium and inorganic phosphorus level were dropped slightly. Each calf was treated for three successive days with magnesium and calcium salts, with tranquilizers, in addition to sodium chloride for relief of dehydration. With the use of these medicaments as compared to the commerical preparations, the frequency of tetanic attacks decreased gradually then disappeared completely after the completion of treatment.
本试验纳入28头患有低镁血症性手足搐缩的自然发病犊牛,通过测定血清中镁、钙、钠、无机磷、钾和SGOT & CPK酶的水平进行临床诊断。血清镁、钙水平显著降低(P<0.01)。血清钾和血清酶水平在破伤风发作期间显著升高(P < 0.01)。血清钠、无机磷水平略有下降。每头小牛都连续三天服用镁盐、钙盐、镇静剂以及氯化钠来缓解脱水。与商业制剂相比,使用这些药物后,破伤风发作的频率逐渐下降,并在治疗完成后完全消失。
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引用次数: 0
Population Genetic Structure of Three Cichlids in ‎Ilorin, North-Central Nigeria 尼日利亚中北部伊洛林地区三种慈鲷的种群遗传结构
Pub Date : 2022-06-29 DOI: 10.30539/ijvm.v46i1.1307
O. A. Iyiola, Rahmat D. Shaibu, S. O. Oladipo, K. Kareem, L. M. Nneji, A. Adeola, A. T. Anifowoshe, M. Matouke
Cichlids are among the economically important which serve as a source of food for people ‎around the world. A deep understanding of the population structure and genetic diversity of ‎cichlids are vital for initiation of conservation policies and sustainable aquaculture. There is ‎paucity of information on the patterns of genetic variations among and within cichlids in ‎North-central Nigeria. This study, therefore, investigated population genetic structure of ‎Coptodon zillii, Oreochromis niloticus, and Hemichromis fasciatus collected from different ‎freshwater bodies in North-central Nigeria. Genomic DNA was extracted, and five highly ‎polymorphic RAPD primers were used for RAPD-PCR amplification and genotyping of the ‎fish. Genetic polymorphism within and between the three tilapia species were examined. ‎Percentages of polymorphism loci, pairwise population matrix, analysis of molecular variance ‎‎(AMOVA), and genetic distances of cichlid populations were determined using standard ‎methods, and dendrograms were constructed using an un-weighted pair group method of ‎arithmetic mean (UPGMA). Overall, percentages of estimated molecular variance within and ‎among C. zillii, H. fasciatus and O. niloticus populations were 5% and 95%; 4% and 96% and ‎‎13% and 87%, respectively. Our results suggest that the three cichlids have close evolutionary ‎relationship and there were no distinct genetic differences on the basis on sampling locations. ‎C. zillii and H. fasciatus are more genetically closer than O. niloticus. This study concludes that ‎RAPD is useful in studying the population genetic structure of cichlids. This study therefore ‎recommends conservation of genetic pool of cichlid species through proper maintenance and ‎restoration of polluted habitat to guarantee sustainable fishery production. However, markers ‎such as microsatellite DNA can be assayed in further studies for better results‎.
慈鲷是经济上重要的鱼类之一,是世界各地人们的食物来源。对慈鲷种群结构和遗传多样性的深入了解对于启动保护政策和可持续水产养殖至关重要。关于尼日利亚中北部慈鲷之间和内部的遗传变异模式的信息缺乏。因此,本研究对尼日利亚中北部不同淡水水体中采集的紫齿象(Coptodon zillii)、nilochromis和fasciatus的种群遗传结构进行了研究。提取基因组DNA,利用5条高多态性RAPD引物进行RAPD- pcr扩增和基因分型。研究了三种罗非鱼的遗传多态性。采用标准方法测定了慈鲷群体的多态性位点百分比、两两群体矩阵、分子方差分析(AMOVA)和遗传距离,并采用非加权的算术平均值对群法(UPGMA)构建了树图。总体而言,紫僵虱、筋膜库蚊和尼罗库蚊种群内部和种群间的分子变异百分比分别为5%和95%;分别为4%和96%,13%和87%。结果表明,这三种慈鲷具有密切的进化关系,在取样地点上没有明显的遗传差异。‎C。zillii和H. fasciatus在基因上比O. niloticus更接近。因此,RAPD在研究慈鲷群体遗传结构方面具有重要意义。因此,本研究建议通过适当的维护和修复受污染的栖息地来保护慈鲷物种基因库,以保证渔业的可持续生产。然而,微卫星DNA等标记物可以在进一步的研究中进行分析,以获得更好的结果。
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引用次数: 1
Evaluation of Antibacterial and Antibiofilm Activity of Biogenic Silver Nanoparticles and Gentamicin Against Staphylococcus aureus Isolated from Caprine Mastitis 生物源银纳米颗粒和庆大霉素对山羊乳腺炎金黄色葡萄球菌的抗菌和抗生物膜活性评价
Pub Date : 2022-06-29 DOI: 10.30539/ijvm.v46i1.1309
A. Al-Dujaily, A. Mahmood
The goal of this study was to assess the antibacterial efficiency of biogenic silver nanoparticles (AgNPs) and gentamicin against Staphylococcus aureus that can form biofilms. The characterization of AgNPs‎ was confirmed by the scanning electron microscope (SEM) which was spherical and homogenous in form, with a diameter between 25 and 45 nm. The X-ray diffraction (XRD) presented the size of AgNPs to be 50 nm. Energy dispersive spectroscopy (EDS) was used to examine the presence of elemental silver. The three-dimensional structure of silver nanoparticles was discovered using an atomic force microscope (AFM), with a diameter of 47.18 nm on average. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of AgNPs and gentamicin against S. aureus isolated from caprine mastitis were determined using the microdilution assay. The checkerboard microdilution technique was utilized to inspect the synergistic antibacterial activity of AgNPs with gentamicin utilizing the fractional inhibitory concentration index (FICI). The antibiofilm capability of AgNPs was also investigated. The results indicate that AgNPs generated by biosynthesis are antibacterial against S. aureus. Moreover, AgNPs and gentamicin exhibit synergistic action. The study's findings suggest that biogenic AgNPs may act as anti-biofilm agents and treat mastitis caused by S. aureus. In conclusions biosynthesized AgNPs exhibit strong antibacterial and antibiofilm effectiveness and synergistic activity when combined with gentamicin.
本研究的目的是评估生物源银纳米颗粒(AgNPs)和庆大霉素对可形成生物膜的金黄色葡萄球菌的抗菌效率。通过扫描电子显微镜(SEM)证实了AgNPs的特征,其形状为球形,形状均匀,直径在25 ~ 45 nm之间。x射线衍射(XRD)显示AgNPs的尺寸为50 nm。利用能量色散光谱(EDS)检测了银元素的存在。利用原子力显微镜(AFM)发现了银纳米颗粒的三维结构,平均直径为47.18 nm。采用微量稀释法测定AgNPs和庆大霉素对山羊乳腺炎金黄色葡萄球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。采用棋盘格微量稀释技术,利用分数抑制浓度指数(FICI)检测AgNPs与庆大霉素的协同抑菌活性。研究了AgNPs的抗菌膜性能。结果表明,生物合成的AgNPs对金黄色葡萄球菌具有抗菌作用。此外,AgNPs和庆大霉素表现出协同作用。该研究结果表明,生物源性AgNPs可能作为抗生物膜剂治疗金黄色葡萄球菌引起的乳腺炎。综上所述,生物合成AgNPs与庆大霉素联用时具有较强的抗菌和抗生物膜效果及协同作用。
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引用次数: 1
The Prevalence of Canine Dipylidiasis in Baghdad city, Iraq‎ ‎ 伊拉克巴格达市犬双螺旋体病的流行病学研究
Pub Date : 2022-06-29 DOI: 10.30539/ijvm.v46i1.1314
Dunya AA Mohammad Salih, A. K. Abbas
Dipylidiasis is a zoonotic intestinal cestode disease caused by Dipylidium caninum. ‎The objective of this study was to investigate the prevalence of Dipylidium caninum infections ‎in dogs from Baghdad city. The study involved the impact of sexes, age, during nine months ‎‎(from January to October 2021) on dipylidiasis infection in pet and stray dogs. 108 faecal ‎samples were collected from (54 pets and 54 stray dogs) then examined for the detection of D. ‎caninum egg capsules and eggs using coprological diagnosis (flotation and sedimentation ‎methods) in the laboratory of parasitology / College of Veterinary Medicine, University of ‎Baghdad. The overall infection rate was 14.81% (16/108). The study revealed the infection rate ‎of D. caninum from pets and stray dogs was 9.26% (5/54), and 20.37% (11/54), respectively. ‎Infection rates in stray dogs were high during March, April, and May (12.5% for each month), ‎compared with pet dogs (0%, 6.25%, 6.25%) respectively, while in January, the rate of ‎infection was 0% in both types, significantly (P<0.05) during March. A significant difference ‎was recorded (P<0.01) between the sexes in both dog types, females recorded a higher 25% ‎‎(3/12) infection rate than males 19.05% (8/42) in stray dogs, compared to the females, 5.9% ‎‎(1/17) and males, 10.8% (4/37) in pet dogs, respectively. According to the age, a high infection ‎rate was found in stray dog adults (20.51%), while the infection rate was zero (0%) in pet dog ‎puppies, with a significant difference (p < 0.01) between the age groups in both types. The ‎results revealed that stray and pet dogs may play a larger role in the spread of zoonotic ‎dipylidiasis and that public health needs more concerted efforts to educate dog owners and ‎implement control programs to prevent the infective stage maturation by cutting life cycle of ‎intermediate hosts.
双螺旋虫病是由犬双螺旋虫引起的一种人畜共患的肠道寄生虫病。本研究的目的是调查巴格达市犬犬双螺旋体感染的流行情况。该研究涉及性别、年龄在9个月内(2021年1月至10月)对宠物和流浪狗双螺旋病感染的影响。收集了(54只宠物和54只流浪狗)108份粪便样本,然后在巴格达大学兽医学院寄生虫学实验室使用粪学诊断(浮选和沉降法)检测犬D.犬卵胶囊和卵。总感染率为14.81%(16/108)。调查结果显示,宠物和流浪狗感染犬D. caninum的比例分别为9.26%(5/54)和20.37%(11/54)。3月、4月和5月流浪狗的感染率较高(每月为12.5%),高于宠物狗(分别为0%、6.25%和6.25%),而1月流浪狗和宠物狗的感染率均为0%,差异有统计学意义(P<0.05)。两种犬的性别差异有统计学意义(P<0.01),其中流浪犬雌性感染率为25%(3/12),高于雄性19.05%(8/42),而宠物犬雌性感染率为5.9%(1/17),雄性感染率为10.8%(4/37)。按年龄分,流浪犬成年犬感染率较高(20.51%),而宠物犬幼犬感染率为0(0%),两种类型年龄组间差异有统计学意义(p < 0.01)。结果表明,流浪狗和宠物狗可能在人畜共患双螺旋病的传播中发挥更大的作用,公共卫生部门需要更多的协调努力来教育狗主人,并实施控制计划,通过缩短中间宿主的生命周期来防止感染阶段的成熟。
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引用次数: 0
Histomorphometric and Histopathological Alterations of Rat Testis Following Exposure to Hydrogen ‎Peroxide: Protective Role of Resveratrol Supplement 大鼠睾丸暴露于过氧化氢后的组织形态学和组织病理学改变:补充白藜芦醇的保护作用
Pub Date : 2022-06-29 DOI: 10.30539/ijvm.v46i1.1313
Jasim M Abdulla, B. N. Al-Okaily
Resveratrol (RS) is widely used in medical fields as an antioxidant. Current research investigated ‎the protective role of RS supplement on histomorphometric and histopathological alterations in ‎testes were caused by hydrogen peroxide (H2O2) of rats as an animal model. Thirty-two adult ‎rats were utilized in this study, divided randomly into 4 equal groups as follows. The group C ‎was given tap water only and served as control, the 2nd group (G1) was given 0.5% of H2O2 of ‎tap water, the 3rd group (G2) was given tap water containing 0.5% of H2O2 and intubated RS ‎supplement at 87 mg/kg BW, and 4th group intubated RS supplement 87 mg/kg BW. after 56 ‎days of treatment, rats were euthanized, dissected then, specimens of testes tissue were collected ‎for histomorphometric and histopathological evaluation. Our results showed that administration ‎of H2O2 caused a significant histomorphometric with histopathological changes in the form of a ‎thickness of fibrous tunica albuginea, disarrangement of germ cells, necrosis of spermatogonia, ‎edema, and loss of sperms as compared to other groups. Meanwhile, these histological alterations ‎were partially attenuated in the G2 group that intubated resveratrol. Thus, the current study ‎concluded that resveratrol may have therapeutic value in the treatment of induced testicular injury ‎by H2O2 due to its antioxidant activity and attenuation of harmful effects of oxidative ‎stress through a mechanism that should be elucidated in future studies.
白藜芦醇(Resveratrol, RS)作为抗氧化剂在医学领域有着广泛的应用。目前研究了RS补充剂对过氧化氢(H2O2)引起的大鼠睾丸组织形态学和组织病理学改变的保护作用。本研究选用32只成年大鼠,随机分为4组:C组只给予自来水作为对照,第二组(G1)给予0.5% H2O2自来水,第三组(G2)给予含0.5% H2O2自来水,并以87 mg/kg BW的剂量插管RS,第四组插管RS 87 mg/kg BW。治疗56 d后,将大鼠安乐死,解剖,取睾丸组织标本进行组织形态学和组织病理学评价。我们的研究结果表明,与其他组相比,H2O2处理引起了显著的组织形态学和组织病理学变化,表现为白膜纤维厚度、生殖细胞紊乱、精原细胞坏死、水肿和精子丢失。同时,这些组织学改变在输注白藜芦醇的G2组部分减弱。因此,本研究认为白藜芦醇在H2O2诱导睾丸损伤的治疗中可能具有一定的治疗价值,其抗氧化活性和抑制氧化应激的有害影响,其机制有待于进一步研究。
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引用次数: 3
Some Teratogenic Outcomes in Rats Exposed to Zinc Chloride Pre and Post Pregnancy 妊娠前后暴露于氯化锌的大鼠的一些致畸结局
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v45i2.1261
Abdul-Rahman Zaher, F. M. Al-Rekabi, Saad Akram Hatif
The aim of present study was to evaluate the possibility of teratogenicity in rats when exposed to zinc chloride (ZnCl2) pre and post pregnancy. To achieve this goal, a total of 40 mature Albino Wistar female rats were divided equally into four groups as follows: T1, dosed 0.7 mg/day ZnCl2 for two months before mating and till to the day 5th of pregnancy, the females of this group were mated with males dosed 0.7 mg/day ZnCl2 for two weeks before mating; T2, dosed 0.7 mg/day ZnCl2 for two months before mating and till to the day 16th of pregnancy and then were mated with control males (not exposed to any level of ZnCl2); T3, dosed 0.7mg/day ZnCl2 for two months before mating and till the end of pregnancy and were mated with control males; Control, dosed with water free from ZnCl2 along the period of experiment and were mated with control males. At the end of each pregnancy phase, results revealed that alpha fetoprotein serum levels were significantly (P<0.05) higher in all treatment groups compared to the control group, and the most prominent increase was observed in the T3 group. All treatment groups showed a significant (P<0.05) decrease in gestation, viability, and lactation indices when compared to the control group, with the T3 group showing the most significant decrease. Additionally, on days 1, 4, 7, 14, and 21 of lactation period, there was a significant (P<0.05) decrease in mean pup body weights in treated groups compared to the control group, with T3 group having the most prominent body weight decrease. The findings of this study revealed that ZnCl2 at a daily dose of 0.7 mg may cause teratogenic defects in rats at various stages of pregnancy, particularly at the third stage. As high-risk groups, pregnant women and children should use Zn supplementation carefully, whether as a food additive or for self-medication. Simultaneously, evaluating effect of low-dose Zn supplementation over a longer duration is required.
本研究的目的是评估大鼠妊娠前后暴露于氯化锌(ZnCl2)的致畸性可能性。为达到这一目的,将40只成年白化Wistar雌性大鼠平均分为4组:T1组,在交配前给药0.7 mg/d的ZnCl2 2个月,直到怀孕第5天,在交配前给药0.7 mg/d的雄性交配2周;T2组,在交配前两个月服用0.7 mg/天的ZnCl2,直到怀孕第16天,然后与对照组雄鼠交配(不暴露于任何水平的ZnCl2);T3组,在交配前2个月至妊娠结束时给药0.7mg/d,与对照雄鼠交配;对照,在实验期间给予不含ZnCl2的水,并与对照雄虫交配。各妊娠期结束时,各治疗组血清α胎蛋白水平均显著高于对照组(P<0.05),其中T3组升高最为显著。与对照组相比,各治疗组妊娠、生存力、泌乳指标均显著降低(P<0.05),其中T3组降低最为显著。在泌乳期第1、4、7、14和21天,各处理组幼犬平均体重均显著低于对照组(P<0.05),其中T3组体重下降最为显著。本研究结果显示,日剂量为0.7 mg的ZnCl2可引起妊娠各阶段大鼠的致畸缺陷,特别是在妊娠第三阶段。作为高危人群,孕妇和儿童应谨慎使用锌补充剂,无论是作为食品添加剂还是自行用药。同时,需要在较长时间内评估低剂量锌补充的效果。
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引用次数: 1
AN ELECTROPHORETIC STUDY ON SERUM PROTEINS IN ARABIAN RACE HORSES NATURALLY INFECTED WITH Babesia equi 自然感染马巴贝斯虫的阿拉伯赛马血清蛋白的电泳研究
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1239
A. Sultan
 Electrophoretic patterns of serum protein in 12 Arabian race horses acutely infected with Babesia equi revealed a significant decrease in albumin (P<0.01) and beta globulins (P<0.05) where, alpha globulins fractions significantly (P<0.01) increased. No significant (P>0.05) changes were recorded in gamma globulins fractions and total serum protein.
12匹急性巴贝斯虫感染的阿拉伯赛马血清蛋白电泳图谱显示,白蛋白含量显著降低(P0.05), γ球蛋白和总血清蛋白含量变化显著。
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引用次数: 0
HISTOMORPHOLOGICAL STUDY ON THE CAROTID SINUS IN RABBIT 家兔颈动脉窦的组织形态学研究
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1241
Ali Hammodi Sadik
Histomorphological study confirmed that the carotid sinus was characterized by an abrupt thinning of elastic tunica media which was rapidly replaced by the normal muscularity of the media on the distal progression in the internal carotid artery. The carotid sinus was richly supplied with sensory receptors which took the form of menisci and left the sinus as the nerve of Hering.
组织形态学研究证实,颈动脉窦的特征是弹性中膜突然变薄,在颈内动脉远端进展时,中膜的正常肌肉组织迅速取代了弹性中膜。颈动脉窦内有丰富的感觉感受器,这些感受器以半月板的形式存在,并留下窦作为Hering的神经。
{"title":"HISTOMORPHOLOGICAL STUDY ON THE CAROTID SINUS IN RABBIT","authors":"Ali Hammodi Sadik","doi":"10.30539/ijvm.v21i1.1241","DOIUrl":"https://doi.org/10.30539/ijvm.v21i1.1241","url":null,"abstract":"Histomorphological study confirmed that the carotid sinus was characterized by an abrupt thinning of elastic tunica media which was rapidly replaced by the normal muscularity of the media on the distal progression in the internal carotid artery. The carotid sinus was richly supplied with sensory receptors which took the form of menisci and left the sinus as the nerve of Hering.","PeriodicalId":22528,"journal":{"name":"The Iraqi Journal of Veterinary Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43908823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
The Iraqi Journal of Veterinary Medicine
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