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EFFECTS OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS ON SHEEP KIDNEYS 假结核棒状杆菌对绵羊肾脏的影响
Pub Date : 2021-11-05 DOI: 10.30539/ijvm.v25i1.1158
M. Hussain
Light and electron microscopic examinations were performed on kidneys of sheep infected with caseous lymphadenitis ( CLA ) caused by Corynebacterium pseudotuberculosis Membranproliferative glomerulonephritis was demonstrated. There were irregular thickenings of the glomeruelar basement membrane and glomerular cell proliferation, in contrast, no such lesions were found in the control lambs, indicating that CLA play a role in inducing glomerulorephritis
对绵羊肾脏进行了光镜和电镜检查,证实绵羊肾脏感染了由假结核棒状杆菌引起的干酪性淋巴结炎(CLA)。肾小球基底膜有不规则增厚和肾小球细胞增殖,而对照羔羊没有发现这种病变,表明CLA在诱导肾小球肾炎中起作用
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引用次数: 0
Protective activity of specific transfer factor against Salmonella typhimurium infection 特定转移因子对鼠伤寒沙门菌感染的保护作用
Pub Date : 2021-11-05 DOI: 10.30539/ijvm.v25i1.1156
A. Yousif, K. Khalifa, S. Al-Samarrae
Specific transfer factor (TF) extracted from spleens of sensitized and non-sensitized guinea pigs to study the efficacy of transfer of cellular immunity specific for salmonella . Two groups each of five guinea pigs were used for in vivo TF preparation. The First group was inoculated with 1 ml of aromatic dependent Salmonella typhimurium SL 1479 vaccine at a dose of 10' cfu/ml intramuscularly twice at two weeks intervals. The second group was injected with trypticase soy broth similarly . These two groups used as a donor for TF, and TFn respectively. Twenty one recipient guinea pigs were divided into three groups, the first group was TF at a dose of 1 ml equivalent to 5X10 cell intramuscularly three times/2 days intervals, Similarly the second group was given TF, where as the third group was given PBS- Cell mediated immunity in recipient animal was evaluated by delayed type hypersensitivity - skin test, Macrophage migration inhibition test (MIF) and then challenged with virulent Salmonella typhimurium. The TFt recipient group induced skin test and showed migration indices less than 0.8 and overcome the challenge organism. Contrary to TFn & PBS recipient groups which did not show any response for skin test and given migration indices more than 0.8 and did not show resistance for virulent Salmonella typhimurium .
从致敏和非致敏豚鼠脾脏中提取特异性转移因子(TF),研究沙门氏菌特异性细胞免疫转移的效果。采用两组,每组5只豚鼠进行体内TF制备。第一组接种芳香依赖性鼠伤寒沙门菌SL 1479疫苗1 ml,剂量为10 cfu/ml,每隔2周肌肉注射2次。第二组同样注射胰酶豆汤。这两组分别作为TF和TFn的供体。将21只受体豚鼠分为三组,第一组给予1 ml相当于5X10细胞的剂量的TF肌肉注射,间隔2天3次,第二组同样给予TF,第三组给予PBS。通过延迟型超敏皮肤试验、巨噬细胞迁移抑制试验(MIF)评估受体动物的细胞介导免疫,然后用毒力强的鼠伤寒沙门菌攻击。TFt受体组诱导皮肤试验,迁移指数小于0.8,克服了攻毒菌。与TFn和PBS受体组相反,TFn和PBS受体组皮肤试验无反应,迁移指数大于0.8,对致病菌鼠伤寒沙门菌无耐药性。
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引用次数: 0
The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats 欧芹种子水提物对实验性大鼠氧化应激的保护作用
Pub Date : 2021-11-05 DOI: 10.30539/ijvm.v25i1.1157
K. K. Khudiar, B. Abdullah, K. A. Al-Mzaien
In this study, the potential protective effect of aqueous extract of parsley (Petroselinum sativum) seeds against hydrogen peroxide (H2O2) – induced oxidative stress in male rats was assessed. Three groups of male albino rats were randomly divided (n=7) and were handled for twenty-eight days as follows: rats in group I served as control; animals in group || were provided with drinking water containing 0.5% H2O2 and those in group III received orally 8 mg/100 gm B.W. of aqueous extract of parsley seeds plus 0.5% H2O2 in drinking water. After four weeks experimental period, a significant increase in lipid peroxidation products (MDA), and decrease in glutathione (GSH) concentrations were observed in plasma, kidney, liver and heart tissues of H2O2 treated animals as compared with the control group. These biomarkers (GSH and MDA) are interrelated and indicate the occurrence of oxidative stress. Plasma total cholesterol (TC) concentration was significantly increased in H2O2 treated rats. By administration of aqueous extract of parsley along with H2O2, plasma and tissue GSH levels were significantly increased while the elevation in MDA level was diminished in plasma and different tissues examined. A decrease in plasma cholesterol concentration was recorded in H2O2 and parsley treated group as compared with the control one and H2O2 treated groups. These results indicate that aqueous extract of parsley have hypocholesterolemic and antioxidant effect.
在本研究中,评估了欧芹种子水提取物对过氧化氢(H2O2)诱导的雄性大鼠氧化应激的潜在保护作用。雄性白化大鼠随机分为三组(n=7),处理28天,如下:I组大鼠作为对照;向||组的动物提供含有0.5%H2O2的饮用水,并且第III组的动物口服8mg/100gm B.W.的欧芹种子水提取物加0.5%H2O2在饮用水中。经过四周的实验期,与对照组相比,在H2O2处理的动物的血浆、肾脏、肝脏和心脏组织中观察到脂质过氧化产物(MDA)显著增加,谷胱甘肽(GSH)浓度显著降低。这些生物标志物(GSH和MDA)是相互关联的,表明氧化应激的发生。H2O2处理的大鼠血浆总胆固醇(TC)浓度显著升高。通过给予欧芹水提取物和H2O2,血浆和组织GSH水平显著升高,而血浆和不同组织中MDA水平的升高则有所降低。与对照组和H2O2处理组相比,H2O2和欧芹处理组的血浆胆固醇浓度降低。这些结果表明,欧芹水提取物具有降胆固醇和抗氧化作用。
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引用次数: 1
Delayed Ovulation in Bovine: Diagnosis and Treatment 牛延迟排卵的诊断与治疗
Pub Date : 2021-11-04 DOI: 10.30539/ijvm.v25i1.1148
Al-Timimi I. H., Khammas D. J., A. M.
Repeated breeder dairy cows (96) were used in this study. They were diagnosed clinically as cases of delayed ovulation and divided into five groups according to type of treatment used post insemination. Group one which includes 28 cow (1=28) treated with 1 vial of fertagyl, (2=22) treated with 2 vials of fertagyl, (3=19) treated with 5 ml of chorionic gonadotropin, (4=17) treated with 1 vial of Nymfalom and (5=10) treated with 1 vial of chorulon. Results showed that the number of cows which did not return to estrus (responded) were 16(57.14%), 12(54.54%), 9(47.47%), 12(70.6%) and 4(40%) respectively. Treatment and insemination were repeated to a number of unresponded cows and their results revealed response of 3 cows (27.27%) from group (1=11), response of 4 cows (50%) from group (2=8), response of 3 cows (42.85%) from group (3=7), response of 1 cow (20%) from group (4=5) and response of 2 (50%) from group (5=4). Data concerning the number, type, sex and viability of the offspring born from the treated cows were included in this study. Finally we may conclude that Nymfalon showed better result in treating cases of delayed ovulation and in improving their conception and pregnancy rate. The remaining treatments were also advisable in the absence of Nymfalon. The use of the same treatment again was also beneficial in some unresponded cases.
本研究选用96头重复种奶牛。临床诊断为延迟排卵病例,根据人工授精后治疗方式分为5组。第一组28头奶牛(1=28头),1瓶fertagyl(2=22头),2瓶fertagyl(22头),5 ml绒毛膜促性腺激素(3=19头),1瓶Nymfalom(4=17头),1瓶chorulon(5=10头)。结果显示,未回发情(应答)奶牛分别为16头(57.14%)、12头(54.54%)、9头(47.47%)、12头(70.6%)和4头(40%)。对未应答的奶牛进行重复处理和授精,结果显示:1=11组有3头奶牛应答(27.27%),2=8组有4头奶牛应答(50%),3=7组有3头奶牛应答(42.85%),4=5组有1头奶牛应答(20%),5=4组有2头奶牛应答(50%)。本研究包括了处理奶牛所生后代的数量、类型、性别和生存能力的数据。结论:宁法龙在治疗延迟排卵患者,提高受孕率和受孕率方面有较好的效果。在没有尼姆法隆的情况下,其余的治疗方法也是可取的。在一些无反应的病例中,再次使用相同的治疗也是有益的。
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引用次数: 1
Comparative Study of Laser- attenuated and Gamma attenuated irradiated larval Vaccines of Dictyocaulus filaria in goats. 山羊丝虫病激光减毒疫苗与γ减毒疫苗的比较研究。
Pub Date : 2021-11-04 DOI: 10.30539/ijvm.v25i1.1149
A. K., A. G
A comparative trial between the effect of 3rd stage Dictyocaulus filaria larval vaccine attenuated by continuous emission of visible Helium – Neon Laser of ImW and wave length of ( 632.8 ) nm of (5) minutes exposure that of gamma attenuated larvae at 0.5 k. gray ( Co as radiation Source ) was designed. Each of the two attenuated larval vaccines was given to a group of ( 5 ) kids each . Double immunization doses at ( 35 ) days interval from each vaccine were given orally. The first dose contained ( 1000 ) larvae and the second one ( 2000 ) for each . A challenge dose of ( 100 ) non- irradiated larvae /kg body weight was given after ( 5 ) week to the vaccinated groups and a control non- vaccinated (5 kids ) 3rd group. All animals were slaughtered after 6 weeks post challenge.  Results showed that both vaccines revealed 80.7% and 78.1% protection respectively for worm burden as compared to control. Laser attenuated vaccine exhibited a statistically significant inhibition in the fecundity of female worms and larvae secretion in faeces in comparison with the gamma irradiated vaccine. 
设计了连续发射ImW可见氦-氖激光减毒的第三阶段丝虫Dictyocaulus幼虫疫苗与0.5 k灰度(Co作为辐射源)γ减毒幼虫暴露(5)分钟的(632.8)nm波长的效果的比较试验。两种减毒幼虫疫苗分别给一组(5)名儿童接种。每种疫苗间隔(35)天口服两次免疫剂量。第一剂含有(1000)只幼虫,第二剂含有(2000)只幼虫。接种组和对照组未接种疫苗(5名儿童)第三组在(5)周后给予(100)只未辐照幼虫/kg体重的攻击剂量。所有动物在挑战后6周后被屠宰。结果显示,与对照组相比,两种疫苗对蠕虫负荷的保护率分别为80.7%和78.1%。与γ辐照疫苗相比,激光减毒疫苗对雌性蠕虫的繁殖力和幼虫在粪便中的分泌表现出统计学上显著的抑制作用。
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引用次数: 0
EFFECT OF PENICILLIN -CIPROFLOXACIN COMBINATION ON SEMEN CHARACTERISTIC AND BACTERIAL CONTAMINANTS OF DILUTED BULL SEMEN 青霉素-环丙沙星联合应用对稀释公牛精液特征及细菌污染物的影响
Pub Date : 2021-11-02 DOI: 10.30539/ijvm.v26i1.1139
N. A. M. Ali, K. I. Al-Badry, S. F. AL-HISSEN, B. A. AL-KAFAGI
Effect of penicillin- ciprofloxacin combinations on some semen characteristics and bacterial contaminants of diluted bull semen was determined and compared with those of penicillin- streptomycin combinations. Sperm motility, percentage of dead and abnormal spermatozoa and survival index did not differ significantly for penicillin (1000 IU) and ciprofloxacin (125, 250, 500, 1000 ug) combinations per milliliter of diluted bull semen from those of penicillin (1000 I.U) and streptomycin (500 ug) combination / ml. Each penicillin ciprofloxacin combination effectively controlled bacterial growth. However penicillin streptomycin combination did not effectively control the growth. Accordingly, the combination of penicillin- ciprofloxacin seems to be a satisfactory substituent for penicillin- streptomycin combination for controlling bacterial contaminants in diluted bull semen without impairing semen characteristics.
测定了青霉素-环丙沙星联合用药对稀释公牛精液的某些特征及细菌污染物的影响,并与青霉素-链霉素联合用药进行了比较。每毫升稀释公牛精液中,青霉素(1000 IU)和环丙沙星(125、250、500、1000 ug)组合与青霉素(1000 IU)和链霉素(500 ug)组合相比,精子活力、死亡和异常精子百分比及存活指数无显著差异,两种青霉素环丙沙星组合均能有效控制细菌生长。然而,青霉素、链霉素联合用药并不能有效控制其生长。因此,青霉素-环丙沙星的组合似乎是一个令人满意的取代青霉素-链霉素组合控制细菌污染稀释公牛精液而不损害精液特征。
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引用次数: 0
THE EVALUATION OF IRAQI PUREBRED ARAB HORSES OVER 12 YEARS 伊拉克纯种阿拉伯马12年的评价
Pub Date : 2021-11-02 DOI: 10.30539/ijvm.v26i1.1140
Sinan A. Al- khazraji
Changes in the exterior purebred Arab horses during the last 12 years have been analyzed. Measurements with regard to the exterior were made on the presently living 238 horses from Baghdad, Nenava and Dialla. The exterior of purebred Arab horses has not been found principally changed: only the length of trunk has become a little longer in stallion and the girths circumference width of chest increased while in mares the length of trunk has become a little shorter and the girths circumference width of chest decreased. Nowadays, the living Iraqi purebred Arab horses present leveled type of horses with harmonious conformation. It seems that a stable. 
分析了过去12年来纯种阿拉伯马外观的变化。对来自巴格达、内纳瓦和迪亚拉的238匹马进行了外观测量。纯种阿拉伯马的外观并没有发生重大变化:种马只有躯干长度变长,胸部围宽增加,而母马的躯干长度变短,胸部围宽度减少。目前,伊拉克现存的纯种阿拉伯马呈现出体型协调的水平型马。看起来很稳定。
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引用次数: 0
PRELIMINARY STUDY ON AQUACULLURE OF CTENOPHARYNGODON IDELLA VAL. 青鳉鱼水产养殖的初步研究。
Pub Date : 2021-11-01 DOI: 10.30539/ijvm.v26i1.1132
J. M. Al-Jawda, A. N. Balasem, K. Asmar, A. S. Ali
The classical method of aquaculture in Iraq employed Cyprinus carpio L. (Common carp) as monoculture or as predominat species togather with grass carp and silver carp. In the present study monoculture of grass carp was performed. Three - hundreds fingerlings of grass carp of 1.5 g weight were cultured in about 600 m2 eartheen pond from 20th of June till 9th of November, 1997. For the first two months of the experiment, no additional feed was added to what had been available in the pond. For the rest of the culture period, 1773 kg of five species of plants were utilized. They were included 91 kg of Phargmites australis, 972 kg of Paspalim sp., 423 kg of Medicago sativa, 171 kg of Ceratophyllum demersum and 116 kg of Paspalum Paspaloides. In addition to that a total of 150 kg of manure was used as a fertilizer in three occasions. At the end of the experiment the average weight of the fishes was 498 gm ranged from 370 gm to 980 gm. Further study was commence where 300 fingerlings of grass carp weighting 34 gm as an average were cultured together with 50 fingerlings of silver carp (average weight 92 gm). The experiment January 1998. For the first two months of experiment the fishes didn't consume began in additional food probably due to the low temperature of water during this period.
伊拉克传统的水产养殖方法是将鲤鱼(鲤鱼)作为单一养殖或与草鱼和鲢鱼一起作为优势种。本研究对草鱼进行了单作养殖。1997年6月20日至11月9日,在约600平方米的泥池中饲养了300尾1.5克重的草鱼鱼苗。在试验的前两个月,没有在池塘中添加任何额外的饲料。其余培养期共利用5种植物1773 kg。其中,南方吸螨91公斤、斑草972公斤、紫花苜蓿423公斤、长尾角菜171公斤、雀稗116公斤。除此之外,总共150公斤的粪肥被三次用作肥料。实验结束时,鱼的平均体重为498克,范围从370克到980克不等。进一步的研究开始了,将300条草鱼鱼苗(平均体重34克)与50条鲢鱼鱼苗(平均体重92克)一起饲养。实验于1998年1月进行。在实验的前两个月,鱼没有进食额外的食物,这可能是由于这段时间水的温度较低。
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引用次数: 0
SINGLE AND MIXED BLOOD PROTOZOA INFECTION WITH ANAPLASMA AND THEILERIA IN BUFFALOES (BUBALUS BUBALIS ) IN BAGHDAD - IRAQ 伊拉克巴格达水牛(BUBALUS BUBALIS)单血和混合血原生动物无浆体和泰勒氏体感染
Pub Date : 2021-11-01 DOI: 10.30539/ijvm.v26i1.1133
S. A. Hasso, Nisreen A. AL - Nashy
One hundred & six blood smears from buffaloes, slaughtered at Al- Futhaliya slaughter house in Baghdad, were examined. Ages of buffaloes ranged from 6 months to 10 years. Numbers of positive blood smears were 48 (45.28%), of them only one showed clinical signs. Single, double, and triple protozoal infections with Anaplasma centrali , Anaplasma marginale, and Theileria annulata, were found. The most common type of infection was the Anaplasma marginale (22.64%), while Anaplasma centrali and Anaplasma centrali + Theileria annulata (0.94%) each. This investigation confirmed the presence of Anaplasma centrali infection in buffaloes for the first time.
对巴格达Al-Futhalya屠宰场屠宰的水牛的106份血液涂片进行了检查。水牛的年龄从6个月到10岁不等。血涂片阳性48例(45.28%),其中只有1例出现临床症状。发现了中央无浆体、边缘无浆体和环状泰勒虫的单、双和三种原生动物感染。最常见的感染类型是边缘无浆体(22.64%),而中央无浆体和中央无浆+环泰勒菌各占0.94%。这项研究首次证实水牛存在中心无浆体感染。
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引用次数: 1
IMMUNOPATHOLOGICAL EFFECT OF SENSITIZED TRANSFER FACTOR ON THE ORGANS OF WHITE MICE AGAINST SALMONELLA TYPHI CHALLENGE INFECTION. 致敏转移因子对小鼠脏器抗伤寒沙门菌侵袭的免疫病理作用。
Pub Date : 2021-11-01 DOI: 10.30539/ijvm.v26i1.1138
K. H. Al-Joboury
In an experimental study to evaluate the immunopathological effect of transfer factor on the reticuloendothelial organs of white mice and their protection against their challenge infection with Salmonella typhi. The results of this study were showed the followings: 1. Transfer factor recipient group: it was showed an early granulomatous lesions in the liver. Reactive hyperplasia in the T cell regions of the spleen and mediastinal lymph node. The early granulomas were persisted during 7th day and slightly regressed on 14th day postinoculation. 2. Infected group with S. typhi: It was showed a multifocal microabscesses consisted of aggregates of neutrophils in the focal area of necrosis, which was evident during 7th day and gradually transform into granulomas on 14th day postinoculation. 3. Transfer factor recipient and challenge infection group: It was showed well developed granulomatous reactions, which indicate an emergence of cellular immunity (delayed type hypersensitivity reaction). These granulomas were more evident on 7th day and slightly regressed on 14th day postinoculation; providing a transfer factor role in tissue reaction and termination of infection.
在一项实验研究中,评估转移因子对小白鼠网状内皮器官的免疫病理作用及其对其挑战性感染伤寒沙门氏菌的保护作用。研究结果表明:1。转移因子受体组:显示早期肝脏肉芽肿性病变。脾脏和纵隔淋巴结T细胞区域的反应性增生。早期肉芽肿在第7天持续存在,在植入后第14天略有消退。2、斑疹伤寒感染组:坏死灶内可见中性粒细胞聚集的多灶性微脓肿,坏死后第7天明显,第14天逐渐转变为肉芽肿。3.转移因子受体和激发感染组:表现出发育良好的肉芽肿性反应,表明出现了细胞免疫(迟发型超敏反应)。这些肉芽肿在第7天更明显,在造瘘后第14天略有消退;提供转移因子在组织反应和终止感染中的作用。
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引用次数: 0
期刊
The Iraqi Journal of Veterinary Medicine
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