Vancomycin and high level gentamicin resistant enterococci detection is important for effective treatment and control of nosocomial infection. The present study was undertaken to determine the species distribution of Enterococcus and the rate of vancomycin and high level gentamicin resistant enterococci (HLGRE) in clinical samples in a tertiary care hospital of Dhaka city. Enterococci were identified to species level by standard biochemical and serological methods. Their susceptibilities to antibiotics were determined by disc diffusion method according to CLSI guideline. Minimum inhibitory concentration (MIC) of vancomycin and gentamicin were determined by agar dilution method. The study was conducted from July 2009 to February 2010. Among 80 isolates, 95% and 5% were identified as Enterococcus faecalis and Enterococcus faecium respectively. Out of 80 isolates 72 (90%) were sensitive and 8 (10%) were intermediate resistant to vancomycin (30μg) by disc diffusion method, but all isolates were susceptible by agar dilution MIC method. Out of 80 enterococci, 37 (46.25%) showed high level resistance to gentamicin (MIC: > 500 μg/ml) by MIC method but, initially six of which showed sensitive result to gentamicin by disc diffusion method using 120 μg disc. The study indicated high prevalence of HLGRE in our hospital population. MIC method was more accurate in detecting high level gentamycin resistant enterococci compared to disc diffusion method with 120 μg gentamicin disc. However, none of the enterococcal strains showed resistance to vancomycin. HLGRE should be monitored regularly in clinical samples as it is difficult to treat. DOI: http://dx.doi.org/10.3329/imcj.v7i2.20103 Ibrahim Med. Coll. J. 2013; 7(2): 28-31
{"title":"HIGH LEVEL GENTAMICIN RESISTANCE AND SUSCEPTIBILITY TO VANCOMYCIN IN ENTEROCOCCI IN A TERTIARY CARE HOSPITAL OF DHAKA CITY","authors":"Shakila Tamanna, L. Barai, A. Ahmed, J. Haq","doi":"10.3329/IMCJ.V7I2.20103","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I2.20103","url":null,"abstract":"Vancomycin and high level gentamicin resistant enterococci detection is important for effective treatment and control of nosocomial infection. The present study was undertaken to determine the species distribution of Enterococcus and the rate of vancomycin and high level gentamicin resistant enterococci (HLGRE) in clinical samples in a tertiary care hospital of Dhaka city. Enterococci were identified to species level by standard biochemical and serological methods. Their susceptibilities to antibiotics were determined by disc diffusion method according to CLSI guideline. Minimum inhibitory concentration (MIC) of vancomycin and gentamicin were determined by agar dilution method. The study was conducted from July 2009 to February 2010. Among 80 isolates, 95% and 5% were identified as Enterococcus faecalis and Enterococcus faecium respectively. Out of 80 isolates 72 (90%) were sensitive and 8 (10%) were intermediate resistant to vancomycin (30μg) by disc diffusion method, but all isolates were susceptible by agar dilution MIC method. Out of 80 enterococci, 37 (46.25%) showed high level resistance to gentamicin (MIC: > 500 μg/ml) by MIC method but, initially six of which showed sensitive result to gentamicin by disc diffusion method using 120 μg disc. The study indicated high prevalence of HLGRE in our hospital population. MIC method was more accurate in detecting high level gentamycin resistant enterococci compared to disc diffusion method with 120 μg gentamicin disc. However, none of the enterococcal strains showed resistance to vancomycin. HLGRE should be monitored regularly in clinical samples as it is difficult to treat. DOI: http://dx.doi.org/10.3329/imcj.v7i2.20103 Ibrahim Med. Coll. J. 2013; 7(2): 28-31","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128562584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sayeed, S. Jahan, M. M. Rhaman, M. M. H. Chowdhury, P. Khanam, T. Begum, U. Ruman, A. Banu, H. Mahtab
Gestational diabetes mellitus (GDM) or hyperglycemia in pregnancy is associated with adverse perinatal outcomes such as large for gestational age (LGA), excess fetal adiposity and cesarean delivery. This study addressed the prevalence of diabetes in pregnancy and to compare the perinatal outcomes between GDM and non-GDM in a rural pregnancy cohort of Bangladesh. Ten villages were purposively selected in a rural area about 100 km off Dhaka City. A population census was conducted. A randomized sample of married women of age 15-45y was drawn from the census data. These women having either regular menstruation (non-regnant) or cessation of menstruation for ≥24weeks (pregnant) were considered eligible. Both the pregnant and non-pregnant women were invited to volunteer the study. Weight, height, waist- and hip-girth and blood pressure were taken. Fasting blood sample was collected for the estimation of plasma glucose (FPG), triglycerides (TG), cholesterol (chol), high-density lipoprotein (HDL). FPG >5.1 mmol/L was taken as cut-off for hyperglycemia in non-pregnant and gestational diabetes mellitus (GDM) for the pregnant women. The biophysical characteristics were compared between pregnant and non-pregnant; and then GDM and non-GDM. Only the pregnant women were taken as a pregnancy cohort. The cohort had followup from 24wks of pregnancy through 28 post-natal days. Results The census yielded 23545 (m / f=11896 / 11649) people of all ages. The married women of age 15-45y were 4526. Of them, 2100 were randomly selected for investigation and 1585 (75.5%) volunteered. The overall prevalence (95% CI) of hyperglycemia (FPG >5.1 mmol/L) was 18.5% (16.7 – 20.3). The prevalence of GDM was 8.9% (7.0 – 10.8) and non-GDM was 19.8% (18.8 – 20.8). The BMI and WHR were significantly higher in the pregnant than non-pregnant women; whereas, there was no significant difference between GDM and non-GDM group. The prevalence rates of abortions, stillbirths, hospital delivery, cesarean delivery, hospital stay ≥7days, puerperal sepsis and neonatal death did not differ between GDM and non-GDM subjects significantly. The prevalence of GDM in rural Bangladesh is comparable with any other population with higher prevalence of GDM. The prevalence of hyperglycemia was found significantly higher in the non-pregnant than the pregnant women. The anthropometric measures did not differ significantly between GDM and non-GDM though FPG was found significantly higher in the former. Compared with the non-GDM the GDM subjects had no significantly higher fetomaternal morbidity and mortality possibly due to non-sedentary habit, non-obesity, non-dyslipidemia or may be due to inherent genetic makeup. A well designed study in a larger sample may explain our findings. DOI: http://dx.doi.org/10.3329/imcj.v7i2.20101 Ibrahim Med. Coll. J. 2013; 7(2): 21-27
{"title":"Prevalence and perinatal outcomes in GDM and non-GDM in a rural pregnancy cohort of Bangladesh","authors":"M. Sayeed, S. Jahan, M. M. Rhaman, M. M. H. Chowdhury, P. Khanam, T. Begum, U. Ruman, A. Banu, H. Mahtab","doi":"10.3329/IMCJ.V7I2.20101","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I2.20101","url":null,"abstract":"Gestational diabetes mellitus (GDM) or hyperglycemia in pregnancy is associated with adverse perinatal outcomes such as large for gestational age (LGA), excess fetal adiposity and cesarean delivery. This study addressed the prevalence of diabetes in pregnancy and to compare the perinatal outcomes between GDM and non-GDM in a rural pregnancy cohort of Bangladesh. Ten villages were purposively selected in a rural area about 100 km off Dhaka City. A population census was conducted. A randomized sample of married women of age 15-45y was drawn from the census data. These women having either regular menstruation (non-regnant) or cessation of menstruation for ≥24weeks (pregnant) were considered eligible. Both the pregnant and non-pregnant women were invited to volunteer the study. Weight, height, waist- and hip-girth and blood pressure were taken. Fasting blood sample was collected for the estimation of plasma glucose (FPG), triglycerides (TG), cholesterol (chol), high-density lipoprotein (HDL). FPG >5.1 mmol/L was taken as cut-off for hyperglycemia in non-pregnant and gestational diabetes mellitus (GDM) for the pregnant women. The biophysical characteristics were compared between pregnant and non-pregnant; and then GDM and non-GDM. Only the pregnant women were taken as a pregnancy cohort. The cohort had followup from 24wks of pregnancy through 28 post-natal days. Results The census yielded 23545 (m / f=11896 / 11649) people of all ages. The married women of age 15-45y were 4526. Of them, 2100 were randomly selected for investigation and 1585 (75.5%) volunteered. The overall prevalence (95% CI) of hyperglycemia (FPG >5.1 mmol/L) was 18.5% (16.7 – 20.3). The prevalence of GDM was 8.9% (7.0 – 10.8) and non-GDM was 19.8% (18.8 – 20.8). The BMI and WHR were significantly higher in the pregnant than non-pregnant women; whereas, there was no significant difference between GDM and non-GDM group. The prevalence rates of abortions, stillbirths, hospital delivery, cesarean delivery, hospital stay ≥7days, puerperal sepsis and neonatal death did not differ between GDM and non-GDM subjects significantly. The prevalence of GDM in rural Bangladesh is comparable with any other population with higher prevalence of GDM. The prevalence of hyperglycemia was found significantly higher in the non-pregnant than the pregnant women. The anthropometric measures did not differ significantly between GDM and non-GDM though FPG was found significantly higher in the former. Compared with the non-GDM the GDM subjects had no significantly higher fetomaternal morbidity and mortality possibly due to non-sedentary habit, non-obesity, non-dyslipidemia or may be due to inherent genetic makeup. A well designed study in a larger sample may explain our findings. DOI: http://dx.doi.org/10.3329/imcj.v7i2.20101 Ibrahim Med. Coll. J. 2013; 7(2): 21-27","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131994417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was designed to determine the extent of drug resistance of M . tuberculosis (MTB) isolated from category II treatment failure pulmonary tuberculosis (PTB) patients. A total of 100 Ziehl-Neelsen (Z-N) smear positive category II failure PTB patients were included in this study. Sputum culture was done in Lowenstein-Jensen (L-J) media. Conventional proportion method on Lowenstein-Jensen (L-J) media was used to determine the drug susceptibility of M. tuberculosis to isoniazid (INH), rifampicin (RMP), ofloxacin (OFX) and kanamycin (KA). Out of 100 sputum samples, a total of 87 samples were positive by culture. Drug susceptibility test (DST) revealed that 82 (94.25%) isolates were resistant to one or more anti -TB drugs. Resistance to isoniazide (INH), rifampicin (RMP), ofloxacin (OFX) and kanamycin (KA) was 94.25%, 82.75%, 29.90% and 3.45% respectively. Among these isolates, 79.31% and 3.45% isolates were multi-drug resistant (MDR) and extended drug resistant (XDR) M. tuberculosis respectively. High rate of anti-tubercular drug resistance was observed among the category II treatment failure TB patients. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17741 Ibrahim Med. Coll. J. 2013; 7(1): 9-11
{"title":"Drug resistance pattern of M. tuberculosis in category II treatment failure pulmonary tuberculosis patients","authors":"F. Rahman, S. Sharmin, M. Kamal, R. Miah","doi":"10.3329/IMCJ.V7I1.17741","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17741","url":null,"abstract":"This study was designed to determine the extent of drug resistance of M . tuberculosis (MTB) isolated from category II treatment failure pulmonary tuberculosis (PTB) patients. A total of 100 Ziehl-Neelsen (Z-N) smear positive category II failure PTB patients were included in this study. Sputum culture was done in Lowenstein-Jensen (L-J) media. Conventional proportion method on Lowenstein-Jensen (L-J) media was used to determine the drug susceptibility of M. tuberculosis to isoniazid (INH), rifampicin (RMP), ofloxacin (OFX) and kanamycin (KA). Out of 100 sputum samples, a total of 87 samples were positive by culture. Drug susceptibility test (DST) revealed that 82 (94.25%) isolates were resistant to one or more anti -TB drugs. Resistance to isoniazide (INH), rifampicin (RMP), ofloxacin (OFX) and kanamycin (KA) was 94.25%, 82.75%, 29.90% and 3.45% respectively. Among these isolates, 79.31% and 3.45% isolates were multi-drug resistant (MDR) and extended drug resistant (XDR) M. tuberculosis respectively. High rate of anti-tubercular drug resistance was observed among the category II treatment failure TB patients. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17741 Ibrahim Med. Coll. J. 2013; 7(1): 9-11","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130549674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acid violence is a barbaric form of violence in Bangladesh. Acid violence also called acid throwing or vitriolage, is defined as the act of throwing of strong corrosives on face and body of a person with the intention of causing permanent disfiguration, intense pain, scarring and sometimes blindness. All of these injuries are considered as ‘grievous hurt’ under section 320 of B.P.C (Bangladesh Penal Code). For the last few years it is on the rise in both urban and rural areas of Bangladesh. The perpetrators are mostly men and adolescent boys. The overwhelming majority of the victims are women and many of them are girls and young females. Recently, however, there have been acid attacks on children, older women and also men. These attacks are often the result of family and land dispute, dowry demands or a desire for revenge due to failure in love affairs or marriage proposals. It is considered as one of the extreme forms of repression and violation of women’s right. This review article is aimed to focus on the present situation of this barbaric act of vengeance against women and young adolescent girls with regard to frequency, causes, long term consequences and creating public awareness on the issue by tightly regulating the sale and transport of acid as well as enacting harsher penalties for perpetrators. Data collection is based on information from Dhaka Medical College hospital, One Stop Crisis Center (OCC), Acid Survivors’ Foundation, Naripokkho Action Aid and several Dhaka based organizations. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17744 Ibrahim Med. Coll. J. 2013; 7(1): 18-20
{"title":"ACID VIOLENCE: A BURNING ISSUE IN BANGLADESH","authors":"Gulshan Akhter, F. Islam","doi":"10.3329/IMCJ.V7I1.17744","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17744","url":null,"abstract":"Acid violence is a barbaric form of violence in Bangladesh. Acid violence also called acid throwing or vitriolage, is defined as the act of throwing of strong corrosives on face and body of a person with the intention of causing permanent disfiguration, intense pain, scarring and sometimes blindness. All of these injuries are considered as ‘grievous hurt’ under section 320 of B.P.C (Bangladesh Penal Code). For the last few years it is on the rise in both urban and rural areas of Bangladesh. The perpetrators are mostly men and adolescent boys. The overwhelming majority of the victims are women and many of them are girls and young females. Recently, however, there have been acid attacks on children, older women and also men. These attacks are often the result of family and land dispute, dowry demands or a desire for revenge due to failure in love affairs or marriage proposals. It is considered as one of the extreme forms of repression and violation of women’s right. This review article is aimed to focus on the present situation of this barbaric act of vengeance against women and young adolescent girls with regard to frequency, causes, long term consequences and creating public awareness on the issue by tightly regulating the sale and transport of acid as well as enacting harsher penalties for perpetrators. Data collection is based on information from Dhaka Medical College hospital, One Stop Crisis Center (OCC), Acid Survivors’ Foundation, Naripokkho Action Aid and several Dhaka based organizations. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17744 Ibrahim Med. Coll. J. 2013; 7(1): 18-20","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122825201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Rashid, H. Rabbi, Md. Amirul Islam, M. Husain, A. Minn, Mohammad Ali
Pancreatolithiasis is a risk factor for developing pancreatic cancer. We report here a rare case of sarcomatoid carcinoma of the pancreas in a 55-year old diabetic male associated with pancreatolithiasis. CT scan of abdomen revealed a large operable mass occupying the distal body and tail of the pancreas. Per-operative survey revealed a small metastatic nodule in the surface of hepatic segment IVa. Histopathology of the distal pancreatic lesion revealed sarcomatoid carcinoma. Hepatic nodule was a metastatic adenocarcinoma. Distal pancreatectomy and splenectomy was done en-mass, along with non-anatomical resection of the hepatic metastatic nodule. Combined with six cycles of chemotherapy, the patient survived a total of another fourteen months. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17742 Ibrahim Med. Coll. J. 2013; 7(1): 12-15
{"title":"A rare case of sarcomatoid carcinoma of the pancreas associated with pancreatolithiasis","authors":"M. Rashid, H. Rabbi, Md. Amirul Islam, M. Husain, A. Minn, Mohammad Ali","doi":"10.3329/IMCJ.V7I1.17742","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17742","url":null,"abstract":"Pancreatolithiasis is a risk factor for developing pancreatic cancer. We report here a rare case of sarcomatoid carcinoma of the pancreas in a 55-year old diabetic male associated with pancreatolithiasis. CT scan of abdomen revealed a large operable mass occupying the distal body and tail of the pancreas. Per-operative survey revealed a small metastatic nodule in the surface of hepatic segment IVa. Histopathology of the distal pancreatic lesion revealed sarcomatoid carcinoma. Hepatic nodule was a metastatic adenocarcinoma. Distal pancreatectomy and splenectomy was done en-mass, along with non-anatomical resection of the hepatic metastatic nodule. Combined with six cycles of chemotherapy, the patient survived a total of another fourteen months. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17742 Ibrahim Med. Coll. J. 2013; 7(1): 12-15","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124147862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salmonella enterica serovar Typhi ( S. Typhi) is occasionally associated with abscess formation in various organs of the body. But breast abscess by S. Typhi without the general and specific symptoms of typhoid fever is unusual. We report a case of breast abscess due to S. Typhi in a 20 year old nonlactating diabetic female without the features of typhoid fever. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17743 Ibrahim Med. Coll. J. 2013; 7(1): 16-17
{"title":"BREAST ABSCESS DUE TO SALMONELLA ENTERICA SEROVAR TYPHI IN A YOUNG DIABETIC FEMALE","authors":"L. Barai, S. Jilani, J. Haq","doi":"10.3329/IMCJ.V7I1.17743","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17743","url":null,"abstract":"Salmonella enterica serovar Typhi ( S. Typhi) is occasionally associated with abscess formation in various organs of the body. But breast abscess by S. Typhi without the general and specific symptoms of typhoid fever is unusual. We report a case of breast abscess due to S. Typhi in a 20 year old nonlactating diabetic female without the features of typhoid fever. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17743 Ibrahim Med. Coll. J. 2013; 7(1): 16-17","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121262646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to evaluate the efficacy of MRSA Chrome agar to detect methicillin resistant Staphylococcus aureus (MRSA) and compare it with 1μg oxacillin disc diffusion tests and detection of mecA gene by PCR. A total 116 Staphylococcus aureus ( S. aureus ), isolated from various clinical samples, were obtained from three tertiary care hospitals of Dhaka city. S. aureus was identified by colony characters, Gram stain and standard biochemical procedures. MRSA was detected by susceptibility to 1μg oxacillin disc, growth of denim blue color colonies of S. aureus on the Brilliance MRSA Chrome agar at 24 and 48 hours of incubation. PCR was performed for amplification of mecA gene as a gold standard method. Out of 116 isolated S. aureus , 33 (28.44%) were MRSA by oxacillin disc diffusion test where mecA gene was detected in 28 strains. On MRSA Chrome agar, 29 (25.0%) S. aureus produced denim blue colonies at 24 hours, of which 28 isolates possessed mecA gene. At 48 hours incubation, an additional 4 isolates yielded denim blue colonies from which mecA gene could not be identified. All the strains of S. aureus that produced denim blue colonies at 24 and 48 hours were resistant to oxacillin. The sensitivity, specificity and accuracy of oxacillin disc diffusion test were 100%, 94.31% and 95.68% and Chrome agar at 24 hours were 100%, 98.86% and 99.13% respectively. Thus MRSA Chrome agar could be good choice in clinical microbiology laboratory for rapid and accurate identification of MRSA. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17697 Ibrahim Med. Coll. J. 2013; 7(1): 1-4
{"title":"Evaluation of MRSA Chrome agar for the detection of methicillin resistant Staphylococcus aureus","authors":"D. Chowdhury, S. T. Jhora, T. Khan, S. Afroz","doi":"10.3329/IMCJ.V7I1.17697","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17697","url":null,"abstract":"The aim of this study was to evaluate the efficacy of MRSA Chrome agar to detect methicillin resistant Staphylococcus aureus (MRSA) and compare it with 1μg oxacillin disc diffusion tests and detection of mecA gene by PCR. A total 116 Staphylococcus aureus ( S. aureus ), isolated from various clinical samples, were obtained from three tertiary care hospitals of Dhaka city. S. aureus was identified by colony characters, Gram stain and standard biochemical procedures. MRSA was detected by susceptibility to 1μg oxacillin disc, growth of denim blue color colonies of S. aureus on the Brilliance MRSA Chrome agar at 24 and 48 hours of incubation. PCR was performed for amplification of mecA gene as a gold standard method. Out of 116 isolated S. aureus , 33 (28.44%) were MRSA by oxacillin disc diffusion test where mecA gene was detected in 28 strains. On MRSA Chrome agar, 29 (25.0%) S. aureus produced denim blue colonies at 24 hours, of which 28 isolates possessed mecA gene. At 48 hours incubation, an additional 4 isolates yielded denim blue colonies from which mecA gene could not be identified. All the strains of S. aureus that produced denim blue colonies at 24 and 48 hours were resistant to oxacillin. The sensitivity, specificity and accuracy of oxacillin disc diffusion test were 100%, 94.31% and 95.68% and Chrome agar at 24 hours were 100%, 98.86% and 99.13% respectively. Thus MRSA Chrome agar could be good choice in clinical microbiology laboratory for rapid and accurate identification of MRSA. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17697 Ibrahim Med. Coll. J. 2013; 7(1): 1-4","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122564035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conjunctival flora refers to population of microorganisms that dwell within the eyes of healthy individuals and is important in maintaining a healthy ocular surface and normal conjunctival function. Conjunctival flora may be altered by a variety of factors that include age, immunosuppression and geography. Immune function is compromised in diabetes mellitus. The aim of the present study was to see the pattern of conjunctival bacterial flora in diabetic and non-diabetic patients. This cross sectional study was carried out in BSMMU during the period of January 2011 to December 2011. Total 500 conjunctival swabs were collected from both eyes of 50 diabetic patients attending OPD of Endocrinology Department of BSMMU and 200 non-diabetic individuals. Significant number of culture was positive in diabetic patients (64.0%) compared to that of non-diabetic individuals (38.0%). Staphylococcus epidermidis was predominant in both study groups (diabetic vs non-diabetic: 41.3% vs 65.26%). Staphylococcus aureus (15.22%), Escherichia coli (6.52%) and Enterobacter (8.33%) were isolated in diabetic patients. Rate of positive culture in both and single eyes were higher in diabetic (28%, 36.0%) than that of non-diabetic individuals (9.5%, 28.5%). DOI: http://dx.doi.org/10.3329/imcj.v7i1.17698 Ibrahim Med. Coll. J. 2013; 7(1): 5-8
结膜菌群是指居住在健康人眼睛内的微生物种群，对维持健康的眼表和正常的结膜功能很重要。结膜菌群可能受到多种因素的影响，包括年龄、免疫抑制和地理位置。糖尿病患者的免疫功能受损。本研究的目的是观察糖尿病和非糖尿病患者结膜细菌菌群的模式。本横断面研究于2011年1月至2011年12月在BSMMU进行。收集50例就诊于BSMMU内分泌科门诊的糖尿病患者和200例非糖尿病患者的双眼结膜拭子共500份。糖尿病患者(64.0%)的培养阳性显著高于非糖尿病患者(38.0%)。表皮葡萄球菌在两个研究组中均占主导地位(糖尿病vs非糖尿病:41.3% vs 65.26%)。糖尿病患者中检出金黄色葡萄球菌(15.22%)、大肠杆菌(6.52%)和肠杆菌(8.33%)。糖尿病患者双眼和单眼培养阳性率(28%，36.0%)高于非糖尿病患者(9.5%，28.5%)。DOI: http://dx.doi.org/10.3329/imcj.v7i1.17698 Ibrahim Med. col。j . 2013;7 (1): 5 - 8
{"title":"Conjunctival bacterial flora in diabetic patients","authors":"N. Nahar, S. Anwar, R. Miah","doi":"10.3329/IMCJ.V7I1.17698","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17698","url":null,"abstract":"Conjunctival flora refers to population of microorganisms that dwell within the eyes of healthy individuals and is important in maintaining a healthy ocular surface and normal conjunctival function. Conjunctival flora may be altered by a variety of factors that include age, immunosuppression and geography. Immune function is compromised in diabetes mellitus. The aim of the present study was to see the pattern of conjunctival bacterial flora in diabetic and non-diabetic patients. This cross sectional study was carried out in BSMMU during the period of January 2011 to December 2011. Total 500 conjunctival swabs were collected from both eyes of 50 diabetic patients attending OPD of Endocrinology Department of BSMMU and 200 non-diabetic individuals. Significant number of culture was positive in diabetic patients (64.0%) compared to that of non-diabetic individuals (38.0%). Staphylococcus epidermidis was predominant in both study groups (diabetic vs non-diabetic: 41.3% vs 65.26%). Staphylococcus aureus (15.22%), Escherichia coli (6.52%) and Enterobacter (8.33%) were isolated in diabetic patients. Rate of positive culture in both and single eyes were higher in diabetic (28%, 36.0%) than that of non-diabetic individuals (9.5%, 28.5%). DOI: http://dx.doi.org/10.3329/imcj.v7i1.17698 Ibrahim Med. Coll. J. 2013; 7(1): 5-8","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126217851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
No abstract available. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17696 Ibrahim Med. Coll. J. 2013; 7(1): i-ii
没有摘要。DOI: http://dx.doi.org/10.3329/imcj.v7i1.17696 Ibrahim Med. col。j . 2013;7 (1): i ii
{"title":"Maternal and childhood undernutrition in Bangladesh: Keeping the issue on the agenda","authors":"M. Mohsena","doi":"10.3329/IMCJ.V7I1.17696","DOIUrl":"https://doi.org/10.3329/IMCJ.V7I1.17696","url":null,"abstract":"No abstract available. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17696 Ibrahim Med. Coll. J. 2013; 7(1): i-ii","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123805592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maternal mortality is an important indicator which reflects the health status of a community. It can be calculated by maternal mortality ratio (MMR), maternal mortality rate (MMRate), and adult life time risk of maternal death. MMR estimates are based on varieties of methods that include household surveys, sisterhood methods, reproductive-age mortality studies (RAMOS), verbal autopsies and censuses. Main causes of maternal mortality are hemorrhage, infection, unsafe abortion, hypertensive disorder of pregnancy and obstructed labour. Factors of maternal mortality have been conceptualized by three delays model. Estimates of maternal mortality ratio (MMR) trend between 1990 and 2010 (over 20 years period) suggest a global reduction (47%), with a greater reduction in developing countries (47%) including Bangladesh than in developed countries (39%). However, to meet the challenge of Fifth Millennium Development Goal (MDG5 i.e. to ensure 75% reduction of MMR by the year 2015), the annual rate of MMR decline and increase of skilled attendant at birth need to be still faster. DOI: http://dx.doi.org/10.3329/imcj.v6i2.14735 Ibrahim Med. Coll. J. 2012; 6(2): 64-69
{"title":"Maternal Mortality - A Public Health Problem","authors":"S. Shirin, S. Nahar","doi":"10.3329/IMCJ.V6I2.14735","DOIUrl":"https://doi.org/10.3329/IMCJ.V6I2.14735","url":null,"abstract":"Maternal mortality is an important indicator which reflects the health status of a community. It can be calculated by maternal mortality ratio (MMR), maternal mortality rate (MMRate), and adult life time risk of maternal death. MMR estimates are based on varieties of methods that include household surveys, sisterhood methods, reproductive-age mortality studies (RAMOS), verbal autopsies and censuses. Main causes of maternal mortality are hemorrhage, infection, unsafe abortion, hypertensive disorder of pregnancy and obstructed labour. Factors of maternal mortality have been conceptualized by three delays model. Estimates of maternal mortality ratio (MMR) trend between 1990 and 2010 (over 20 years period) suggest a global reduction (47%), with a greater reduction in developing countries (47%) including Bangladesh than in developed countries (39%). However, to meet the challenge of Fifth Millennium Development Goal (MDG5 i.e. to ensure 75% reduction of MMR by the year 2015), the annual rate of MMR decline and increase of skilled attendant at birth need to be still faster. DOI: http://dx.doi.org/10.3329/imcj.v6i2.14735 Ibrahim Med. Coll. J. 2012; 6(2): 64-69","PeriodicalId":226732,"journal":{"name":"Ibrahim Medical College Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130911923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：