Pub Date : 2017-01-01DOI: 10.6116/KJH.2017.32.2.57
A. Lee, Gyo‐Nam Kim, kimhaeok, W. J. Song, S. Roh
Objectives : Yam bean (Pachyrhizus erosus) possess various nutrients, it has been widely used as traditional cosmetic material in Indonesia. The aim of this study was to investigate the anti-oxidant activity and the anti-melanogenic effect of Yambean (Pachyrhizus erosus) extract and its fractions. Methods : The anti-oxidant activity of yam bean extract assessed based on total polyphenol, flavonoid contents, DPPH and ABTS radical scavenging assay. To evaluate anti-melanogenic effects and cytotoxicity of Yambean extract and its fractions, B16F10 melanoma cell was used. Results : In results, total polyphenol content of yam bean water extract (YW) and Yambean 70% ethanol extract (YE) were 1.18±0.03 mg/g (mg of gallic acid/g of sample), 1.16±0.01 mg/g. Total flavonoid contents of YW, YE were 3.55±0.06 mg/g (mg of naringin/g of sample), 1.78±0.03 mg/g. Moreover, YE scavenged DPPH and ABTS effectively in 4 mg/ml compared to YW. Cytotoxicity of YE and its fractions in B16F10 melanoma cell was measured using MTT assays. It had no cytotoxicity up to 500 μg/ml. Melanin accumulation in B16F10 melanoma cell was induced using alpha-melanocyte stimulating hormone (α-MSH) and 3-isobutyl-1-methylxanthine (IBMX). B16F10 melanoma cell treated with 10-500 μg/ml YE and hexane, ethyl acetate, butanol, H2O fractions for 24h. Non treated B16F10 melanoma cell (Control) markedly increased melanin contents. In contrast, YE ethylacetate fraction effectively suppressed melanin accumulation in a dose-dependent manner. Conclusion : In conclusion, these results suggest that Yambean extract has the potential as a cosmetic material which possess anti-oxidant and anti-melanogenic activities.
{"title":"Antioxidant Activity and Melanin Inhibitory Effects of Yambean (Pachyrhizus erosus) Extract","authors":"A. Lee, Gyo‐Nam Kim, kimhaeok, W. J. Song, S. Roh","doi":"10.6116/KJH.2017.32.2.57","DOIUrl":"https://doi.org/10.6116/KJH.2017.32.2.57","url":null,"abstract":"Objectives : Yam bean (Pachyrhizus erosus) possess various nutrients, it has been widely used as traditional cosmetic material in Indonesia. The aim of this study was to investigate the anti-oxidant activity and the anti-melanogenic effect of Yambean (Pachyrhizus erosus) extract and its fractions. Methods : The anti-oxidant activity of yam bean extract assessed based on total polyphenol, flavonoid contents, DPPH and ABTS radical scavenging assay. To evaluate anti-melanogenic effects and cytotoxicity of Yambean extract and its fractions, B16F10 melanoma cell was used. Results : In results, total polyphenol content of yam bean water extract (YW) and Yambean 70% ethanol extract (YE) were 1.18±0.03 mg/g (mg of gallic acid/g of sample), 1.16±0.01 mg/g. Total flavonoid contents of YW, YE were 3.55±0.06 mg/g (mg of naringin/g of sample), 1.78±0.03 mg/g. Moreover, YE scavenged DPPH and ABTS effectively in 4 mg/ml compared to YW. Cytotoxicity of YE and its fractions in B16F10 melanoma cell was measured using MTT assays. It had no cytotoxicity up to 500 μg/ml. Melanin accumulation in B16F10 melanoma cell was induced using alpha-melanocyte stimulating hormone (α-MSH) and 3-isobutyl-1-methylxanthine (IBMX). B16F10 melanoma cell treated with 10-500 μg/ml YE and hexane, ethyl acetate, butanol, H2O fractions for 24h. Non treated B16F10 melanoma cell (Control) markedly increased melanin contents. In contrast, YE ethylacetate fraction effectively suppressed melanin accumulation in a dose-dependent manner. Conclusion : In conclusion, these results suggest that Yambean extract has the potential as a cosmetic material which possess anti-oxidant and anti-melanogenic activities.","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"3 1","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86193539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.6116/KJH.2017.32.3.71
A. Choi, Hyun-Seo Lee, J. Kim, B. Cho, J. Shin, Seung-Il Jeong, S. Jang
{"title":"Studies on Anti-Inflammatory and Anti-Melanogenic Effect of Grape Fruit Stem Extract","authors":"A. Choi, Hyun-Seo Lee, J. Kim, B. Cho, J. Shin, Seung-Il Jeong, S. Jang","doi":"10.6116/KJH.2017.32.3.71","DOIUrl":"https://doi.org/10.6116/KJH.2017.32.3.71","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"2 1","pages":"71-78"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80038948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.6116/KJH.2017.32.4.33
Minji An, Yeong-Chul Park
{"title":"Acute toxicity test and safety classification for Termitomyces albuminosus containing pharmacologically similar ingredient of Aconitum koreanum","authors":"Minji An, Yeong-Chul Park","doi":"10.6116/KJH.2017.32.4.33","DOIUrl":"https://doi.org/10.6116/KJH.2017.32.4.33","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"31 1","pages":"33-38"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85122386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Antioxidant and Anti-diabetic Effects of Sappan Lignum by Extraction Method","authors":"Y. Hong, Gyeong Han Jeong, Y. H. Jeong, K. Hoon","doi":"10.6116/KJH.2017.32.6.1","DOIUrl":"https://doi.org/10.6116/KJH.2017.32.6.1","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"26 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80914478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-30DOI: 10.6116/KJH.2016.31.6.73
Ji-Seung Lee, M. Kwon, Sungjoon Kweon, Jeeho Kim, J. Moon, Yoonchul Cho, Jung-Won Shin, Jong-Soo Lee, N. Sohn
{"title":"Effects of 18β-glycyrrhetinic acid on pro-inflammatory cytokines and neuronal apoptosis in the hippocampus of lipopolysaccharide-treated mice","authors":"Ji-Seung Lee, M. Kwon, Sungjoon Kweon, Jeeho Kim, J. Moon, Yoonchul Cho, Jung-Won Shin, Jong-Soo Lee, N. Sohn","doi":"10.6116/KJH.2016.31.6.73","DOIUrl":"https://doi.org/10.6116/KJH.2016.31.6.73","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"47 1","pages":"73-81"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73753222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-30DOI: 10.6116/KJH.2016.31.6.21
Y. Song, J. Chang, Ji-Hyun Yoo
{"title":"Antioxidant Activities of Water Extracts from Different Parts of Lycopus lucidus Turcz. ex Benth.","authors":"Y. Song, J. Chang, Ji-Hyun Yoo","doi":"10.6116/KJH.2016.31.6.21","DOIUrl":"https://doi.org/10.6116/KJH.2016.31.6.21","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"211 1","pages":"21-28"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75874854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-30DOI: 10.6116/KJH.2016.31.6.29
Ho-Yyoung Lee, Eun Hyoung Shim, Myeong Soo Lee, J. A. Lee
{"title":"Anti-adipogenesis Effects of 3 Herbal Formula on Blood Stasis","authors":"Ho-Yyoung Lee, Eun Hyoung Shim, Myeong Soo Lee, J. A. Lee","doi":"10.6116/KJH.2016.31.6.29","DOIUrl":"https://doi.org/10.6116/KJH.2016.31.6.29","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"20 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77714027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-30DOI: 10.6116/KJH.2016.31.6.45
Jun-wei Kang, J. Chang, Ji-Hyun Yoo, Eun-Soo Doh, K. Kil
Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were 24.6 mg/ml and 14.3 mg/ml, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.
{"title":"Antioxidant Activities of Extracts from Different Parts of Sasa borealis","authors":"Jun-wei Kang, J. Chang, Ji-Hyun Yoo, Eun-Soo Doh, K. Kil","doi":"10.6116/KJH.2016.31.6.45","DOIUrl":"https://doi.org/10.6116/KJH.2016.31.6.45","url":null,"abstract":"Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were 24.6 mg/ml and 14.3 mg/ml, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"55 1","pages":"45-52"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90054671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Choi, Se‐Jin Kim, In-su Kim, Ji-Beom Lee, Jong-Beom Kim, S. Moon, Hwa-Dong Lee
Objectives : Gamisoyosan (GMS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also, GMS has been used traditionally to improve systemic circulation and biological energy production. The purpose of this study was to assess the anti-oxidant activity and anti-inflammatory effects of Gamisoyosan Formulation (Soft extract, GMS-SE). Methods : The biological activities such as anti-oxidant and anti-inflammatory effects were measured through cell line-based in vitro assay. We investigated the anti-oxidant properties of GMS-SE on the 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, contents of total flavonoid and polyphenol. GMS-SE compared to butyl hydroxy anizole (BHA). Furthermore, based on this result the anti-inflammatory effects of GMS-SE have verified by mechanism from LPS-treated Raw264.7 macrophages. Results : The anti-oxidant activities of GMS-SE increased markedly, in a dose-dependent manner. The GMS-SE showed significant scavenging activity (GMS-SE 500 ㎍/㎖ : 32.77±1.65%, GMS-SE 1000 ㎍/㎖ : 45.06±1.04% and BHA 100 ㎍/㎖ : 39.25±2.41% for DPPH assay). and, The total phenolic compound and flavonoids contents of GMS-SE were 73.93±6.87 ㎍/㎎ and 698.75±6.78 ㎍/㎎. GMS-SE which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Moreover, GMS-SE inhibited the activation of phosphorylation of p38 and ERK MAPKs by induced LPS. Conclusion : The present results indicate that GMS-SE has an anti-oxidant and anti-inflammatory properties, therefore may be beneficial in diseases which related to oxidative stress-mediated inflammatory disorders.
{"title":"Evaluation on Anti-oxidant Activity and Anti-inflammatory Effects for the New Formulation of Gamisoyosan","authors":"H. Choi, Se‐Jin Kim, In-su Kim, Ji-Beom Lee, Jong-Beom Kim, S. Moon, Hwa-Dong Lee","doi":"10.6116/KJH.2016.31.6.1","DOIUrl":"https://doi.org/10.6116/KJH.2016.31.6.1","url":null,"abstract":"Objectives : Gamisoyosan (GMS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also, GMS has been used traditionally to improve systemic circulation and biological energy production. The purpose of this study was to assess the anti-oxidant activity and anti-inflammatory effects of Gamisoyosan Formulation (Soft extract, GMS-SE). Methods : The biological activities such as anti-oxidant and anti-inflammatory effects were measured through cell line-based in vitro assay. We investigated the anti-oxidant properties of GMS-SE on the 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, contents of total flavonoid and polyphenol. GMS-SE compared to butyl hydroxy anizole (BHA). Furthermore, based on this result the anti-inflammatory effects of GMS-SE have verified by mechanism from LPS-treated Raw264.7 macrophages. Results : The anti-oxidant activities of GMS-SE increased markedly, in a dose-dependent manner. The GMS-SE showed significant scavenging activity (GMS-SE 500 ㎍/㎖ : 32.77±1.65%, GMS-SE 1000 ㎍/㎖ : 45.06±1.04% and BHA 100 ㎍/㎖ : 39.25±2.41% for DPPH assay). and, The total phenolic compound and flavonoids contents of GMS-SE were 73.93±6.87 ㎍/㎎ and 698.75±6.78 ㎍/㎎. GMS-SE which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Moreover, GMS-SE inhibited the activation of phosphorylation of p38 and ERK MAPKs by induced LPS. Conclusion : The present results indicate that GMS-SE has an anti-oxidant and anti-inflammatory properties, therefore may be beneficial in diseases which related to oxidative stress-mediated inflammatory disorders.","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"1 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86465996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-30DOI: 10.6116/KJH.2016.31.6.37
J. Y. Lee, Bu-Il Seo, S. Roh
{"title":"Improving effect of Artemisiae Capillaris Herba extract in reflux esophagitis rats","authors":"J. Y. Lee, Bu-Il Seo, S. Roh","doi":"10.6116/KJH.2016.31.6.37","DOIUrl":"https://doi.org/10.6116/KJH.2016.31.6.37","url":null,"abstract":"","PeriodicalId":22911,"journal":{"name":"The Korea Journal of Herbology","volume":"1 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79880632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：