首页 > 最新文献

Toxicological Review最新文献

英文 中文
Methodical approaches to scale-up measurement of glyphosate concentration using HPLC-FLD 采用HPLC-FLD放大测量草甘膦浓度的方法
Pub Date : 2022-12-30 DOI: 10.47470/0869-7922-2022-30-6-395-402
Natalya E. Fedorova, A. A. Ivchenkova, Nataliya Andreevna Stepanova, S. D. Dobrev
Introduction. Traditionally, the hygienic assessment of working conditions when using glyphosate preparations, an extremely popular herbicide in the world, is based on the use of thin layer chromatography. In this paper, alternative approaches are considered. On the basis of experimental data, the conditions for the analysis of glyphosate content in air by high performance liquid chromatography were optimized. Material and methods. All work with glyphosate solutions was carried out in polypropylene vessels. Sampling from the air medium was carried out on a medium filtration filter (“blue ribbon”). Subsequent extraction was carried out with water. The measurements were performed by high performance liquid chromatography with a fluorimetric detector at an excitation wavelength of 270 nm and an emission wavelength of 313 nm. To transfer glyphosate to a molecule with fluorimetric properties after derivatization of glyphosate in an alkaline medium using 9-fluorenylmethyl chloroformate by heating and washing off the excess of the reagent with toluene. A C18 reverse phase column was used; ammonium acetate with the addition of acetic acid and acetonitrile were used as eluents. Results. Approbation of the developed technique was carried out on real samples taken during ground treatment of fallow fields and lands of industrial territories with glyphosate preparations. The detected levels of glyphosate did not exceed the lower limit of quantitative determination: 0.5 mg/m3 in the air of the working area and 0.025 mg/m3 in the atmospheric air (with maximum allowable concentrations of 1.0 and 0.1 mg/m3, respectively). Limitations. The study considers a limited number of chromatographic columns. The study is performed on 25 model samples of the air of the working area and atmospheric air. Conclusion. Based on the results of the work performed, methodological instructions “Measurement of glyphosate concentrations in the air by high-performance liquid chromatography” were formed and sent for metrological certification in the approved manner.
介绍。传统上,当使用草甘膦制剂(一种世界上非常流行的除草剂)时,对工作条件的卫生评估是基于薄层色谱法的使用。本文考虑了几种替代方法。在实验数据的基础上,优化了高效液相色谱法分析空气中草甘膦含量的条件。材料和方法。所有使用草甘膦溶液的工作都在聚丙烯容器中进行。在介质过滤过滤器(“蓝带”)上对空气介质进行采样。随后用水提取。采用高效液相色谱法,荧光检测器,激发波长为270 nm,发射波长为313 nm。用9-氟酰氯甲酸甲酯在碱性介质中衍生化草甘膦后,用甲苯加热并洗去过量的试剂,将草甘膦转化为具有荧光特性的分子。采用C18反相色谱柱;以乙酸铵加乙酸和乙腈为洗脱剂。结果。采用草甘膦制剂对休耕地和工业地区土地进行地面处理时取得的实际样品对所开发的技术进行了批准。检测到的草甘膦含量均未超过定量检测下限:工作区空气中草甘膦含量为0.5 mg/m3,大气中草甘膦含量为0.025 mg/m3(最大允许浓度分别为1.0和0.1 mg/m3)。的局限性。该研究考虑了有限数量的色谱柱。对25个工作区域空气和大气空气的模型样本进行了研究。结论。根据所执行的工作结果,形成了“用高效液相色谱法测量空气中草甘膦浓度”的方法说明,并以批准的方式发送计量认证。
{"title":"Methodical approaches to scale-up measurement of glyphosate concentration using HPLC-FLD","authors":"Natalya E. Fedorova, A. A. Ivchenkova, Nataliya Andreevna Stepanova, S. D. Dobrev","doi":"10.47470/0869-7922-2022-30-6-395-402","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-6-395-402","url":null,"abstract":"Introduction. Traditionally, the hygienic assessment of working conditions when using glyphosate preparations, an extremely popular herbicide in the world, is based on the use of thin layer chromatography. In this paper, alternative approaches are considered. On the basis of experimental data, the conditions for the analysis of glyphosate content in air by high performance liquid chromatography were optimized. Material and methods. All work with glyphosate solutions was carried out in polypropylene vessels. Sampling from the air medium was carried out on a medium filtration filter (“blue ribbon”). Subsequent extraction was carried out with water. The measurements were performed by high performance liquid chromatography with a fluorimetric detector at an excitation wavelength of 270 nm and an emission wavelength of 313 nm. To transfer glyphosate to a molecule with fluorimetric properties after derivatization of glyphosate in an alkaline medium using 9-fluorenylmethyl chloroformate by heating and washing off the excess of the reagent with toluene. A C18 reverse phase column was used; ammonium acetate with the addition of acetic acid and acetonitrile were used as eluents. Results. Approbation of the developed technique was carried out on real samples taken during ground treatment of fallow fields and lands of industrial territories with glyphosate preparations. The detected levels of glyphosate did not exceed the lower limit of quantitative determination: 0.5 mg/m3 in the air of the working area and 0.025 mg/m3 in the atmospheric air (with maximum allowable concentrations of 1.0 and 0.1 mg/m3, respectively). Limitations. The study considers a limited number of chromatographic columns. The study is performed on 25 model samples of the air of the working area and atmospheric air. Conclusion. Based on the results of the work performed, methodological instructions “Measurement of glyphosate concentrations in the air by high-performance liquid chromatography” were formed and sent for metrological certification in the approved manner.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76929704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
About combination of positive and negative outcomes of a subchronic exposure of rats to selenium oxide nanoparticles 关于大鼠亚慢性暴露于氧化硒纳米颗粒的阳性和阴性结果的组合
Pub Date : 2022-12-30 DOI: 10.47470/0869-7922-2022-30-6-386-394
Y. Ryabova, I. Minigalieva, L. Privalova, M. Sutunkova, R. R. Sakhautdinova, S. Klinova, A. V. Tazhigulova, T. Bushueva, O. Makeev, B. Katsnelson
Introduction. Occupational contact with selenium and its compounds, including nanoscale forms, occurs in the glass production, rubber industry, metallurgy (metallurgical processes of copper sludge processing, copper pyrite roasting, manganese, selenium and tellurium production). There are scarce data on the toxicity of selenium nanoparticles. Material and methods. Stable suspensions of nanoparticles or deionized water (control group) were administered to male rats 3 times a week for 6 weeks. A single dose of selenium oxide nanoparticles was 0.2 or 1 or 2 mg/kg of body weight). The condition of the animal organism was assessed with a number of indicators of toxic action at the end of the experiment. The statistical significance of intergroup differences was assessed by Student's t-test. Results. Activity of succinate dehydrogenase in blood lymphocytes reflecting the intensity of energy processes in the organism was decreased. The number of eosinophils in smears-imprints of parenchymal organs and mesenteric lymph nodes increased, indicating the ability of selenium nanooxide to trigger signaling cascades in immunocompetent cells. The number of degenerated cells in the proximal and distal tubules in smears of the kidneys was increased. A tendency to a decrease in all hemodynamics parameters was found. A change in the QT duration, together with an increase in the amplitude of the T wave, probably indicates a violation of the processes of myocardial repolarization. The coefficient of fragmentation of genomic DNA in nucleated blood cells decreased. Limitations. The research was limited to the study of indicators of toxic action in only one study using a limited dose range. Conclusion. An ambiguous effect of selenium oxide nanoparticles on rats was found. Along with negative impact of nanoparticles we have demonstrated, for the first time, some beneficial outcomes, in particular, genome -protective action which is in a striking contrast with the genotoxicity of all elemental and element-oxide nanoparticles previously studied in our laboratory.
介绍。与硒及其化合物(包括纳米级形式)的职业接触发生在玻璃生产、橡胶工业、冶金(铜污泥处理、铜黄铁矿焙烧、锰、硒和碲生产的冶金过程)。关于纳米硒的毒性研究资料很少。材料和方法。将稳定的纳米颗粒悬浮液或去离子水(对照组)给予雄性大鼠,每周3次,持续6周。单剂量的氧化硒纳米颗粒为0.2或1或2毫克/公斤体重)。在实验结束时,用一些毒性作用指标来评估动物机体的状况。组间差异的统计学意义采用Student’st检验。结果。血液淋巴细胞中琥珀酸脱氢酶的活性降低,反映了机体能量过程的强度。实质器官和肠系膜淋巴结涂片印迹中的嗜酸性粒细胞数量增加,表明纳米氧化硒能够触发免疫活性细胞的信号级联反应。肾涂片近端和远端小管变性细胞增多。所有血流动力学参数均有下降趋势。QT持续时间的改变,加上T波振幅的增加,可能表明心肌复极过程的破坏。有核血细胞中基因组DNA的断裂系数降低。的局限性。该研究仅限于毒性作用指标的研究,仅在一项研究中使用了有限的剂量范围。结论。发现氧化硒纳米颗粒对大鼠的影响不明确。随着纳米颗粒的负面影响,我们首次证明了一些有益的结果,特别是基因组保护作用,这与我们实验室先前研究的所有元素和元素氧化物纳米颗粒的遗传毒性形成鲜明对比。
{"title":"About combination of positive and negative outcomes of a subchronic exposure of rats to selenium oxide nanoparticles","authors":"Y. Ryabova, I. Minigalieva, L. Privalova, M. Sutunkova, R. R. Sakhautdinova, S. Klinova, A. V. Tazhigulova, T. Bushueva, O. Makeev, B. Katsnelson","doi":"10.47470/0869-7922-2022-30-6-386-394","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-6-386-394","url":null,"abstract":"Introduction. Occupational contact with selenium and its compounds, including nanoscale forms, occurs in the glass production, rubber industry, metallurgy (metallurgical processes of copper sludge processing, copper pyrite roasting, manganese, selenium and tellurium production). There are scarce data on the toxicity of selenium nanoparticles. Material and methods. Stable suspensions of nanoparticles or deionized water (control group) were administered to male rats 3 times a week for 6 weeks. A single dose of selenium oxide nanoparticles was 0.2 or 1 or 2 mg/kg of body weight). The condition of the animal organism was assessed with a number of indicators of toxic action at the end of the experiment. The statistical significance of intergroup differences was assessed by Student's t-test. Results. Activity of succinate dehydrogenase in blood lymphocytes reflecting the intensity of energy processes in the organism was decreased. The number of eosinophils in smears-imprints of parenchymal organs and mesenteric lymph nodes increased, indicating the ability of selenium nanooxide to trigger signaling cascades in immunocompetent cells. The number of degenerated cells in the proximal and distal tubules in smears of the kidneys was increased. A tendency to a decrease in all hemodynamics parameters was found. A change in the QT duration, together with an increase in the amplitude of the T wave, probably indicates a violation of the processes of myocardial repolarization. The coefficient of fragmentation of genomic DNA in nucleated blood cells decreased. Limitations. The research was limited to the study of indicators of toxic action in only one study using a limited dose range. Conclusion. An ambiguous effect of selenium oxide nanoparticles on rats was found. Along with negative impact of nanoparticles we have demonstrated, for the first time, some beneficial outcomes, in particular, genome -protective action which is in a striking contrast with the genotoxicity of all elemental and element-oxide nanoparticles previously studied in our laboratory.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"52 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88418830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acute poisoning with ваrbituric acid derivatves. Barbituric coma ваrbituric酸性衍生物急性中毒。巴比妥昏迷
Pub Date : 2022-12-30 DOI: 10.47470/0869-7922-2022-30-6-359-369
Vladimir Alexandrovsky, Mariya Vladimirovna Kareva, P. Rozhkov
Introduction. Aim of the study. To summarize the authors’ many years of experience in the diagnosis and treatment of acute poisoning with barbituric acid derivatives and to present the results of a detailed study of the clinic of barbituric intoxication, which prove a toxic damaging effect on the body, causing a deep inhibition of the functions of the central nervous system (CNS) with a predominant effect on the cortex and autonomic centers of the brain trunk. Material and methods. The analysis of 385 patients with acute poisoning with barbiturates of varying severity who were treated in the emergency toxicology department of the N.V. Sklifosovsky Research Institute for Emergency Medicine was carried out. The clinical and encephalographic picture of acute poisoning was assessed. The EEG data of two groups of patients were considered and analyzed, with the possibility of determining the type of bioelectric activity of the brain. The informative value of these types of EEG for a physician is in comparison with the characteristics of clinical and toxicological data. Results. Summarizing the obtained results of clinical and electroencephalographic changes in barbiturate poisoning, it was noted that the identified types of brain bioelectrical activity correspond to certain clinical symptoms and indicate different degrees of poisoning severity. This makes it possible for a practicing physician, after recording an EEG in a patient in a coma, to make a preliminary diagnosis of acute barbiturate poisoning, to purposefully conduct a toxicological study, and to begin specific treatment before receiving an analysis. Analeptic bemegrid causes a direct stimulating effect on the central nervous system. With the introduction of small doses of bеmegrid, a moderate activation of the bioelectrical activity of the brain was noted. Our experience in treating patients with this poisoning showed a negative effect of large doses of bemegrid on the central nervous system, which can lead to an inhibitory phase of parabiotic inhibition (according to N.E. Vvedensky). Conclusion. Poisoning with barbituric acid derivatives currently poses a serious danger to the life and health of patients. This makes the issue of timely diagnosis and treatment measures in the early stages of the disease relevant.
介绍。研究的目的。总结作者多年来对巴比妥酸衍生物急性中毒的诊断和治疗经验,并详细介绍巴比妥中毒的临床研究结果,证明巴比妥中毒对身体有毒性损害作用,引起中枢神经系统功能的深度抑制,主要作用于脑干皮层和自主神经中枢。材料和方法。对在N.V. Sklifosovsky急诊医学研究所急诊毒理学部接受治疗的385例不同严重程度的巴比妥类药物急性中毒患者进行了分析。评估急性中毒的临床和脑电图。对两组患者的脑电图数据进行考虑和分析,有可能确定大脑的生物电活动类型。这些类型的脑电图对医生的信息价值是与临床和毒理学数据的特征进行比较。结果。总结巴比妥酸盐中毒的临床和脑电图变化的结果,指出已确定的脑生物电活动类型与某些临床症状相对应,并表明不同程度的中毒严重程度。这使得执业医生可以在记录昏迷患者的脑电图后,对急性巴比妥酸盐中毒做出初步诊断,有目的地进行毒理学研究,并在接受分析之前开始特定治疗。催眠药对中枢神经系统产生直接刺激作用。随着小剂量的bembromegrid的引入,人们注意到大脑生物电活动的适度激活。我们治疗这种中毒患者的经验表明,大剂量bemegrid对中枢神经系统有负面影响,可导致异种生物抑制的抑制阶段(根据N.E. Vvedensky)。结论。巴比妥酸衍生物中毒目前已严重危及患者的生命和健康。这使得在疾病早期阶段及时诊断和治疗措施的问题具有相关性。
{"title":"Acute poisoning with ваrbituric acid derivatves. Barbituric coma","authors":"Vladimir Alexandrovsky, Mariya Vladimirovna Kareva, P. Rozhkov","doi":"10.47470/0869-7922-2022-30-6-359-369","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-6-359-369","url":null,"abstract":"Introduction. Aim of the study. To summarize the authors’ many years of experience in the diagnosis and treatment of acute poisoning with barbituric acid derivatives and to present the results of a detailed study of the clinic of barbituric intoxication, which prove a toxic damaging effect on the body, causing a deep inhibition of the functions of the central nervous system (CNS) with a predominant effect on the cortex and autonomic centers of the brain trunk. Material and methods. The analysis of 385 patients with acute poisoning with barbiturates of varying severity who were treated in the emergency toxicology department of the N.V. Sklifosovsky Research Institute for Emergency Medicine was carried out. The clinical and encephalographic picture of acute poisoning was assessed. The EEG data of two groups of patients were considered and analyzed, with the possibility of determining the type of bioelectric activity of the brain. The informative value of these types of EEG for a physician is in comparison with the characteristics of clinical and toxicological data. Results. Summarizing the obtained results of clinical and electroencephalographic changes in barbiturate poisoning, it was noted that the identified types of brain bioelectrical activity correspond to certain clinical symptoms and indicate different degrees of poisoning severity. This makes it possible for a practicing physician, after recording an EEG in a patient in a coma, to make a preliminary diagnosis of acute barbiturate poisoning, to purposefully conduct a toxicological study, and to begin specific treatment before receiving an analysis. Analeptic bemegrid causes a direct stimulating effect on the central nervous system. With the introduction of small doses of bеmegrid, a moderate activation of the bioelectrical activity of the brain was noted. Our experience in treating patients with this poisoning showed a negative effect of large doses of bemegrid on the central nervous system, which can lead to an inhibitory phase of parabiotic inhibition (according to N.E. Vvedensky). Conclusion. Poisoning with barbituric acid derivatives currently poses a serious danger to the life and health of patients. This makes the issue of timely diagnosis and treatment measures in the early stages of the disease relevant.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79069887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The use of neurotoxins in fundamental, medical and biological sciences on the example of the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine 神经毒素在基础科学、医学和生物科学中的应用,以1-甲基-4-苯基-1,2,3,6-四氢吡啶为例
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-307-314
V. V. Safandeev, T. A. Sinitskaya
Introduction. Neurotoxins are a class of chemicals that damage neurons. Some pesticides have a similar effect. The increase in areas with developed heavy industry and agriculture, uncontrolled use of pesticides contribute to the emergence and progression of Alzheimer’s and Parkinson’s disease (PD). The insidiousness of PD is that it is still incurable, since there is no early diagnosis: for decades it has been asymptomatic, and by the time of manifestation there are no target neurons for therapy. Considering that the diagnosis is made at the late stages of PD development, the treatment of the disease is ineffective. It follows that in order to increase the effectiveness of treatment, it should be started as early as possible - before the appearance of motor symptoms, at the preclinical stage. To do this, it is first necessary to develop an early (preclinical) diagnosis of PD based on an in-depth study of the cellular and molecular mechanisms of pathogenesis. In order to develop early diagnosis and preventive therapy of PD, proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used in this study. Material and methods. To simulate the preclinical and clinical stages of PD, inbred lines of animals were injected with MPTP according to certain schemes. The behavior of experimental animals was assessed, and after their decapitation, the content of monoamines in the structures of the brain, peripheral organs, and blood plasma was determined by HPLC, and a morphofunctional analysis of the studied tissues and organs was performed. Results. The clinical stage of PD was modeled in animals, which corresponds to the key characteristics of PD in humans. A hypothetical preclinical stage of PD was modeled in animals, on which the method of early diagnosis was successfully tested. This model shows a number of important biochemical changes in peripheral organs, indicating the systemic nature of the disease and preceding the onset of the clinical stage of PD. Research limitations. The results of this study can only be applied to humans only after similar results have been obtained in monkeys. Limitations. Only after obtaining similar results in monkeys the results of this study can be translated to humans. Conclusion. Knowledge about the mechanisms of action of some neurotoxins is expanding, which are successfully used for the needs of fundamental, medical and biological sciences.
介绍。神经毒素是一类破坏神经元的化学物质。一些杀虫剂也有类似的效果。在重工业和农业发达地区,不加控制地使用农药导致阿尔茨海默病和帕金森病(PD)的出现和发展。PD的阴险之处在于,它仍然是无法治愈的,因为没有早期诊断:几十年来,它一直没有症状,到表现出来的时候,没有治疗的目标神经元。考虑到诊断是在PD发展的后期阶段做出的,因此对疾病的治疗是无效的。因此,为了提高治疗的有效性,应该尽早开始-在运动症状出现之前,在临床前阶段。要做到这一点,首先需要在深入研究发病机制的细胞和分子机制的基础上,对PD进行早期(临床前)诊断。为了开展PD的早期诊断和预防治疗,本研究采用神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)。材料和方法。为了模拟帕金森病的临床前和临床阶段,按照一定的方案给自交系动物注射MPTP。对实验动物的行为进行评估,斩首后用高效液相色谱法测定脑、外周器官和血浆结构中的单胺含量,并对所研究的组织和器官进行形态功能分析。结果。PD的临床阶段以动物为模型,符合PD在人类中的关键特征。在动物模型上建立了一个假设的临床前阶段PD,并在此基础上成功地测试了早期诊断方法。该模型显示外周器官的许多重要生化变化,表明疾病的全身性,并在PD临床阶段开始之前。研究的局限性。只有在猴子身上取得了类似的结果,这项研究的结果才能应用于人类。的局限性。只有在猴子身上获得类似的结果,这项研究的结果才能转化为人类。结论。关于某些神经毒素的作用机制的知识正在扩大,这些知识已成功地用于基础科学、医学和生物科学的需要。
{"title":"The use of neurotoxins in fundamental, medical and biological sciences on the example of the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine","authors":"V. V. Safandeev, T. A. Sinitskaya","doi":"10.47470/0869-7922-2022-30-5-307-314","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-307-314","url":null,"abstract":"Introduction. Neurotoxins are a class of chemicals that damage neurons. Some pesticides have a similar effect. The increase in areas with developed heavy industry and agriculture, uncontrolled use of pesticides contribute to the emergence and progression of Alzheimer’s and Parkinson’s disease (PD). The insidiousness of PD is that it is still incurable, since there is no early diagnosis: for decades it has been asymptomatic, and by the time of manifestation there are no target neurons for therapy. Considering that the diagnosis is made at the late stages of PD development, the treatment of the disease is ineffective. It follows that in order to increase the effectiveness of treatment, it should be started as early as possible - before the appearance of motor symptoms, at the preclinical stage. To do this, it is first necessary to develop an early (preclinical) diagnosis of PD based on an in-depth study of the cellular and molecular mechanisms of pathogenesis. In order to develop early diagnosis and preventive therapy of PD, proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used in this study. Material and methods. To simulate the preclinical and clinical stages of PD, inbred lines of animals were injected with MPTP according to certain schemes. The behavior of experimental animals was assessed, and after their decapitation, the content of monoamines in the structures of the brain, peripheral organs, and blood plasma was determined by HPLC, and a morphofunctional analysis of the studied tissues and organs was performed. Results. The clinical stage of PD was modeled in animals, which corresponds to the key characteristics of PD in humans. A hypothetical preclinical stage of PD was modeled in animals, on which the method of early diagnosis was successfully tested. This model shows a number of important biochemical changes in peripheral organs, indicating the systemic nature of the disease and preceding the onset of the clinical stage of PD. Research limitations. The results of this study can only be applied to humans only after similar results have been obtained in monkeys. Limitations. Only after obtaining similar results in monkeys the results of this study can be translated to humans. Conclusion. Knowledge about the mechanisms of action of some neurotoxins is expanding, which are successfully used for the needs of fundamental, medical and biological sciences.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79153715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of principles for coding production and consumption waste by hazardous properties (carcinogenicity, mutagenicity and reprotoxicity) 制定按危险特性(致癌性、诱变性和生殖毒性)对生产和消费废物进行编码的原则
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-332-342
D. N. Rabikova, Khalidya Khizbulaevna Khamidulina, E. Tarasova
Introduction. The world practice of classifying wastes according to the degree of danger involves the simultaneous consideration of the physicochemical, toxic and ecotoxic properties of the waste components. According to Directive 2008/98/EC, waste is divided into hazardous, non-hazardous and mirror, i.e. waste that can be classified as hazardous or non-hazardous depending on the percentage of components. In order to implement part 2 of subparagraph "d" of paragraph 1 of the list of instructions of the President of the Russian Federation dated January 24, 2017 No. Pr-140GS on the harmonization of legislation in the field of environmental protection and legislation in the field of ensuring the sanitary and epidemiological welfare of the population and the development of unified approaches to the classification of waste with taking into account the world experience Russian Register of Potentially Hazardous Chemical and Biological Substances together with the Federal state budgetary institution "Ural state research institute of regional environmental problems", carried out research work to identify wastes with carcinogenic, mutagenic and reprotoxic properties. The aim of the study was to prepare lists of substances contained in production and consumption waste that have a carcinogenic, mutagenic and reprotoxic effects, indicating the forms and preferred route of entry into the body, and develop a methodology for coding waste by hazardous factors. Materials and methods. The analysis of domestic and foreign normative-legal acts on the regulation of waste hazards has been carried out. Results. A method for coding production and consumption wastes has been developed, taking into account carcinogenic, mutagenic and reprotoxic effects. The 905 of the 6130 wastes included in the FWC were coded according to these types of hazard, which requires a revision of their classification. Conclusion. The introduction of the developed coding methodology into the practice of waste management will make it possible to harmonize the classifications of wastes according to their impact on human health and the environment. Coding is an effective tool for waste management regulation, including disposal, recycling and reuse.
介绍。根据危险程度对废物进行分类的世界惯例涉及同时考虑废物成分的物理化学、毒性和生态毒性。根据指令2008/98/EC,废物分为危险、无害和镜像,即废物可根据成分的百分比分为危险或无害。为了执行2017年1月24日俄罗斯联邦总统指示清单第1段“d”项第2部分。关于协调环境保护领域的立法和确保人口卫生和流行病福利领域的立法以及在考虑到世界经验的情况下制定统一的废物分类方法的Pr-140GS俄罗斯潜在危险化学和生物物质登记处与联邦国家预算机构“乌拉尔国家区域环境研究所”问题”进行了研究工作,以确定具有致癌、诱变和生殖毒性的废物。这项研究的目的是编制生产和消费废物中所含具有致癌、诱变和生殖毒性作用的物质清单,说明其进入人体的形式和首选途径,并制订一种按危险因素对废物进行编码的方法。材料和方法。对国内外有关废物危害规制的规范性法律法规进行了分析。结果。考虑到致癌性、诱变性和生殖毒性影响,已经制定了一种对生产和消费废物进行编码的方法。在列入FWC的6130种废物中,有905种是根据这些类型的危害进行编码的,这需要对其分类进行修订。结论。在废物管理实践中采用已开发的编码方法,将有可能根据废物对人类健康和环境的影响统一废物分类。编码是废物管理法规的有效工具,包括处置、回收和再利用。
{"title":"Development of principles for coding production and consumption waste by hazardous properties (carcinogenicity, mutagenicity and reprotoxicity)","authors":"D. N. Rabikova, Khalidya Khizbulaevna Khamidulina, E. Tarasova","doi":"10.47470/0869-7922-2022-30-5-332-342","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-332-342","url":null,"abstract":"Introduction. The world practice of classifying wastes according to the degree of danger involves the simultaneous consideration of the physicochemical, toxic and ecotoxic properties of the waste components. According to Directive 2008/98/EC, waste is divided into hazardous, non-hazardous and mirror, i.e. waste that can be classified as hazardous or non-hazardous depending on the percentage of components. In order to implement part 2 of subparagraph \"d\" of paragraph 1 of the list of instructions of the President of the Russian Federation dated January 24, 2017 No. Pr-140GS on the harmonization of legislation in the field of environmental protection and legislation in the field of ensuring the sanitary and epidemiological welfare of the population and the development of unified approaches to the classification of waste with taking into account the world experience Russian Register of Potentially Hazardous Chemical and Biological Substances together with the Federal state budgetary institution \"Ural state research institute of regional environmental problems\", carried out research work to identify wastes with carcinogenic, mutagenic and reprotoxic properties. The aim of the study was to prepare lists of substances contained in production and consumption waste that have a carcinogenic, mutagenic and reprotoxic effects, indicating the forms and preferred route of entry into the body, and develop a methodology for coding waste by hazardous factors. Materials and methods. The analysis of domestic and foreign normative-legal acts on the regulation of waste hazards has been carried out. Results. A method for coding production and consumption wastes has been developed, taking into account carcinogenic, mutagenic and reprotoxic effects. The 905 of the 6130 wastes included in the FWC were coded according to these types of hazard, which requires a revision of their classification. Conclusion. The introduction of the developed coding methodology into the practice of waste management will make it possible to harmonize the classifications of wastes according to their impact on human health and the environment. Coding is an effective tool for waste management regulation, including disposal, recycling and reuse.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73851359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The effect of the combined effects of carbon monoxide and harmful physical factors on the behavioral activity of animals 一氧化碳和有害物理因素共同作用对动物行为活动的影响
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-297-306
V. E. Kriyt, V. L. Reinyuk, Yu. N. Sladkova, Alexander O. Pyatibrat
Introduction. In the course of their work, firefighters are exposed to many harmful and dangerous factors, among which the chemical factor takes the leading place. Carbon monoxide is an integral part of any combustion, and its leading role in the toxic effect of combustion products is confirmed by numerous experimental data. The results obtained in the experiment on animals are of great importance in the study of combined effects. The purpose of the study was to study the behavioral activity of animals under combined exposure to carbon monoxide and harmful physical factors. Material and methods. The study was performed on outbred white male rats 3 months of age, in two directions: the study of the combined effects of carbon monoxide and elevated air temperature and the study of the combined effects of carbon monoxide and vibroacoustic factor. In each of the experimental models, rats were randomly divided into 6 groups: the first group was exposed to a single physical factor under study, the second group was exposed to multiple physical factors (for 14 days), the third group was exposed to a single exposure to carbon monoxide, the fourth group was exposed to multiple exposures to monoxide carbon, the fifth group was subjected to a single combined effect of a physical factor and carbon monoxide; the sixth group was subjected to multiple combined effects of the physical factor and carbon monoxide. The study of the behavioral activity of animals was carried out using the “Open field” and “Elevated plus maze” methods. Results. The data obtained indicate that the combined effect of carbon monoxide and harmful physical factors causes a change in the indicators of behavioral reactions of animals, while if with a single exposure most of the indicators return to the background values in a day, then with repeated exposure they remain significantly changed. Conclusion. The data obtained can be applied in studying the long-term effects of the combined effects of carbon monoxide and harmful physical factors on the body of firefighters.
介绍。消防队员在工作过程中会接触到许多有害危险因素,其中以化学因素居首。一氧化碳是任何燃烧的组成部分,它在燃烧产物的毒性作用中的主导作用已被大量实验数据所证实。动物实验所得结果对联合效应的研究具有重要意义。本研究的目的是研究动物在一氧化碳和有害物理因素共同作用下的行为活动。材料和方法。本研究以3个月龄的远交种白种雄性大鼠为研究对象,从一氧化碳与气温升高的联合效应研究和一氧化碳与振动声因子的联合效应研究两个方向进行。在每个实验模型中,大鼠被随机分为6组:第一组暴露于单一物理因素,第二组暴露于多种物理因素(14天),第三组暴露于单一一氧化碳暴露,第四组暴露于多重一氧化碳暴露,第五组受到单一物理因素和一氧化碳的联合作用;第六组受物理因素和一氧化碳多重联合作用。采用“开阔场”和“高架+迷宫”两种方法对动物的行为活动进行了研究。结果。所获得的数据表明,一氧化碳与有害物理因素的共同作用会引起动物行为反应指标的变化,而单次暴露后,大部分指标在一天内恢复到背景值,而多次暴露后,这些指标仍有明显变化。结论。所得数据可用于研究一氧化碳与有害物理因素共同作用对消防员身体的长期影响。
{"title":"The effect of the combined effects of carbon monoxide and harmful physical factors on the behavioral activity of animals","authors":"V. E. Kriyt, V. L. Reinyuk, Yu. N. Sladkova, Alexander O. Pyatibrat","doi":"10.47470/0869-7922-2022-30-5-297-306","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-297-306","url":null,"abstract":"Introduction. In the course of their work, firefighters are exposed to many harmful and dangerous factors, among which the chemical factor takes the leading place. Carbon monoxide is an integral part of any combustion, and its leading role in the toxic effect of combustion products is confirmed by numerous experimental data. The results obtained in the experiment on animals are of great importance in the study of combined effects. The purpose of the study was to study the behavioral activity of animals under combined exposure to carbon monoxide and harmful physical factors. Material and methods. The study was performed on outbred white male rats 3 months of age, in two directions: the study of the combined effects of carbon monoxide and elevated air temperature and the study of the combined effects of carbon monoxide and vibroacoustic factor. In each of the experimental models, rats were randomly divided into 6 groups: the first group was exposed to a single physical factor under study, the second group was exposed to multiple physical factors (for 14 days), the third group was exposed to a single exposure to carbon monoxide, the fourth group was exposed to multiple exposures to monoxide carbon, the fifth group was subjected to a single combined effect of a physical factor and carbon monoxide; the sixth group was subjected to multiple combined effects of the physical factor and carbon monoxide. The study of the behavioral activity of animals was carried out using the “Open field” and “Elevated plus maze” methods. Results. The data obtained indicate that the combined effect of carbon monoxide and harmful physical factors causes a change in the indicators of behavioral reactions of animals, while if with a single exposure most of the indicators return to the background values in a day, then with repeated exposure they remain significantly changed. Conclusion. The data obtained can be applied in studying the long-term effects of the combined effects of carbon monoxide and harmful physical factors on the body of firefighters.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73806017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of guidelines for the control of lead content in paint and varnishes 制定控制油漆和清漆中铅含量的准则
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-323-331
N. Fedorova, Marina Valentinovna Egorova, Aleksandr Sergeevich Rodionov, Yuliya Yurevna Bogdanova, Khalidya Khizbulaevna Khamidulina, E. Tarasova, Andrey Konstantinovich Nazarenko
Introduction. Determination of low concentrations of lead in paint and varnishes is one of the actual problems in ensuring the safety of these products. The requirements set out in the draft Technical Regulation of the Eurasian Economic Union «on the Safety of Paint and Coating Materials» for a standard of 0.009% lead on a dry weight basis stipulate the availability of precision methods for determining lead in paint and varnishes with low detection limits. Materials and methods. Nine different types of paint samples were selected as test objects. The study was carried out by atomic absorption spectrometry with electrothermal atomisation with pre-microwave sample preparation. Paint samples were preliminarily applied on a sheet of sanded FC plywood, dried and taken from the surface with a spatula in the form of fine shavings. The absolutely dry chips were microwaved, centrifuged to separate the undecomposed sludge and analysed. The efficiency of the developed approach was proven by validating the method on 20 model samples of one of the paint types investigated. Results. Validation of the proposed method of lead determination in paints gave satisfactory results in terms of recovery rate and repeatability, so it can be concluded that the method is suitable for the solution of the posed analytical problem. Having analysed the results obtained by measuring lead content in 9 samples of paint products according to the proposed scheme in the described methodology, one can make a confident conclusion about the absence of exceeding the maximum permissible lead content, considering the expected reduction of the standard to 0.009%. Conclusion. The usage of the proposed method will make it possible to successfully control the lead content in paintwork materials at levels that comply with international standards and, in the long term, ensure that the requirements of the Technical Regulation of the Eurasian Economic Union «on the Safety of Paint and Coating Materials» are met in terms of reducing the regulatory indicator to 0.009%.
介绍。油漆和清漆中低浓度铅的测定是确保这些产品安全的实际问题之一。欧亚经济联盟《油漆和涂料材料安全》技术法规草案中对干重含铅量为0.009%的标准提出了要求,规定了用于测定油漆和清漆中含铅量的低检测限精确方法的可用性。材料和方法。选取9种不同类型的涂料样品作为测试对象。采用电热雾化原子吸收光谱法对样品进行预微波制备。将油漆样品初步涂在磨砂的FC胶合板上,干燥后用刮刀从表面取下细屑。绝对干燥的碎片被微波,离心分离未分解的污泥和分析。通过对其中一种涂料类型的20个模型样品进行验证,证明了所开发方法的有效性。结果。对所建立的涂料中铅的测定方法进行了验证,在回收率和重复性方面取得了令人满意的结果,因此可以得出结论,该方法适用于解决所提出的分析问题。根据上述方法中的建议方案,分析了9个油漆产品样本的铅含量测量结果后,考虑到预期标准将降至0.009%,我们可以自信地得出结论,没有超过最大允许铅含量。结论。使用拟议的方法将有可能成功地将油漆材料中的铅含量控制在符合国际标准的水平,并且从长远来看,确保欧亚经济联盟“油漆和涂层材料安全”技术法规的要求,将监管指标降低到0.009%。
{"title":"Development of guidelines for the control of lead content in paint and varnishes","authors":"N. Fedorova, Marina Valentinovna Egorova, Aleksandr Sergeevich Rodionov, Yuliya Yurevna Bogdanova, Khalidya Khizbulaevna Khamidulina, E. Tarasova, Andrey Konstantinovich Nazarenko","doi":"10.47470/0869-7922-2022-30-5-323-331","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-323-331","url":null,"abstract":"Introduction. Determination of low concentrations of lead in paint and varnishes is one of the actual problems in ensuring the safety of these products. The requirements set out in the draft Technical Regulation of the Eurasian Economic Union «on the Safety of Paint and Coating Materials» for a standard of 0.009% lead on a dry weight basis stipulate the availability of precision methods for determining lead in paint and varnishes with low detection limits. Materials and methods. Nine different types of paint samples were selected as test objects. The study was carried out by atomic absorption spectrometry with electrothermal atomisation with pre-microwave sample preparation. Paint samples were preliminarily applied on a sheet of sanded FC plywood, dried and taken from the surface with a spatula in the form of fine shavings. The absolutely dry chips were microwaved, centrifuged to separate the undecomposed sludge and analysed. The efficiency of the developed approach was proven by validating the method on 20 model samples of one of the paint types investigated. Results. Validation of the proposed method of lead determination in paints gave satisfactory results in terms of recovery rate and repeatability, so it can be concluded that the method is suitable for the solution of the posed analytical problem. Having analysed the results obtained by measuring lead content in 9 samples of paint products according to the proposed scheme in the described methodology, one can make a confident conclusion about the absence of exceeding the maximum permissible lead content, considering the expected reduction of the standard to 0.009%. Conclusion. The usage of the proposed method will make it possible to successfully control the lead content in paintwork materials at levels that comply with international standards and, in the long term, ensure that the requirements of the Technical Regulation of the Eurasian Economic Union «on the Safety of Paint and Coating Materials» are met in terms of reducing the regulatory indicator to 0.009%.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78171432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic toxicology in hygiene 卫生中的遗传毒理学
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-271-276
N. Ilyushina
The article discusses the directions of research in the field of genetic toxicology, which allow solving problems important for hygiene, aimed at ensuring the genetic health of the population. The main areas of research are: assessment of the genetic hazard of environmental factors and the level of pollution by genotoxicants; analysis of the total mutagenic activity of mixtures of chemical pollutants; control over mutational events in the somatic cells of people working in hazardous production conditions, which is necessary to improve the prevention, diagnosis and treatment of occupational diseases and multifactorial pathology; study of individual genetically determined human sensitivity to the impacts of adverse environmental factors in order to improve professional selection and development of personalized preventive medicine; assessment of mutagenicity in short-term tests to predict the carcinogenicity of the studied substances; search for antimutagens. The methods used in genetic toxicology are described. The main directions and results of the work of the Department of Genetic Toxicology of the FBES “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Rospotrebnadzor are given. Conclusion. The results of genotoxicological studies allow us to evaluate and establish hazard classes of the tested substances, predict possible long-term effects. Monitoring of mutational events in the somatic cells of people exposed to various environmental factors, assessment of individual human sensitivity to specific factors or their combinations make it possible to identify and evaluate potentially dangerous factors for genetic health, develop preventive measures and carry out reasonable professional selection of persons to work in harmful production conditions.
本文讨论了遗传毒理学领域的研究方向,以解决重要的卫生问题,旨在确保人群的遗传健康。主要研究领域有:环境因素遗传危害评价和基因毒物污染水平评价;化学污染物混合物的总诱变活性分析控制危险生产条件下工作人员体细胞突变事件,为提高职业病和多因素病理的预防、诊断和治疗水平所必需;研究个体基因决定的人类对不利环境因素影响的敏感性,以提高专业选择和个性化预防医学的发展;在预测研究物质致癌性的短期试验中评估突变性;寻找抗诱变剂。介绍了遗传毒理学的研究方法。介绍了俄罗斯联邦卫生科学中心遗传毒理学部的主要工作方向和研究成果。结论。基因毒理学研究的结果使我们能够评估和确定测试物质的危害等级,预测可能的长期影响。监测暴露于各种环境因素的人体细胞中的突变事件,评估个人对特定因素或其组合的敏感性,从而有可能确定和评价影响遗传健康的潜在危险因素,制定预防措施,并对在有害生产条件下工作的人员进行合理的专业选择。
{"title":"Genetic toxicology in hygiene","authors":"N. Ilyushina","doi":"10.47470/0869-7922-2022-30-5-271-276","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-271-276","url":null,"abstract":"The article discusses the directions of research in the field of genetic toxicology, which allow solving problems important for hygiene, aimed at ensuring the genetic health of the population. The main areas of research are: assessment of the genetic hazard of environmental factors and the level of pollution by genotoxicants; analysis of the total mutagenic activity of mixtures of chemical pollutants; control over mutational events in the somatic cells of people working in hazardous production conditions, which is necessary to improve the prevention, diagnosis and treatment of occupational diseases and multifactorial pathology; study of individual genetically determined human sensitivity to the impacts of adverse environmental factors in order to improve professional selection and development of personalized preventive medicine; assessment of mutagenicity in short-term tests to predict the carcinogenicity of the studied substances; search for antimutagens. The methods used in genetic toxicology are described. The main directions and results of the work of the Department of Genetic Toxicology of the FBES “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Rospotrebnadzor are given. Conclusion. The results of genotoxicological studies allow us to evaluate and establish hazard classes of the tested substances, predict possible long-term effects. Monitoring of mutational events in the somatic cells of people exposed to various environmental factors, assessment of individual human sensitivity to specific factors or their combinations make it possible to identify and evaluate potentially dangerous factors for genetic health, develop preventive measures and carry out reasonable professional selection of persons to work in harmful production conditions.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88639394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative evaluation of the genotoxicity of carbendazim technical grade active ingredients in the Ames test and micronucleus in vivo test 多菌灵工业级活性成分在Ames试验和微核体内试验中遗传毒性的比较评价
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-277-285
O. Egorova, N. S. Averyanova, L. A. Kara, Natalya Alexandrovna Ilyushina
Introduction. Carbendazim is a systemic benzimidazole fungicide used against a wide range of crop diseases. The ability of carbendazim to induce the incidence of chromosomal aberrations and micronuclei in mammalian cells by influencing the processes of mitotic spindle formation in the cell cycle have been shown in various in vitro and in vivo tests. Contradictory data were obtained in the bacterial test system Salmonella/microsomes, indicating both the absence and the presence of mutagenic activity of carbendazim. The discrepancy in the results may stem from the presence of impurities. The aim of the study was a comparative evaluation of the genotoxicity of various technical products of carbendazim. Materials and methods. The genotoxicity of carbendazim was studied using the plate incorporation version of the Ames test on 5 strains of Salmonella typhimurium in the presence and the absence of metabolic activation system (+S9/-S9) and in a micronucleus test in CD-1 mice. Two technical grade active ingredients (TGAI) and an analytical standard for carbendazim were tested. Results. In the Ames test, the analytical standard of carbendazim possessed no mutagenic activity (±S9) on any of the strains. The most pronounced mutagenic effect was observed for the TGAI I in TA98 strain, the number of revertants at the maximum concentration was 5-7 times higher than that in the negative control. The positive effects of carbendazim TGAIs in the Ames test are likely mediated by the presence of impurities. Under in vivo conditions, all tested TGAIs of carbendazim induced a statistically significant and dose-dependent formation of micronuclei in polychromatic erythrocytes (PCE) of mouse bone marrow. The mean frequency of PCE with micronuclei at the maximum dose exceeded this rate in the negative control by 21-24 times. Research limitations. The study is limited to testing the mutagenic activity of two samples of carbendazim technical products and one sample of its analytical standard in both in vivo and in vitro tests. Conclusion. Taking into account the high content of the active substance in the tested TGAIs, the bacterial reverse mutation test is a highly sensitive method for assessment of the equivalence of carbendazim generic products. The use of a micronucleus test for evaluating of the equivalence of carbendazim TGAIs to the original substance is inappropriate due to the pronounced aneugenic effect.
介绍。多菌灵是一种全身性苯并咪唑类杀菌剂,用于防治多种作物病害。多菌灵通过影响细胞周期中有丝分裂纺锤体形成的过程,在哺乳动物细胞中诱导染色体畸变和微核发生的能力已在各种体外和体内试验中得到证实。在细菌检测系统沙门氏菌/微粒体中得到矛盾的数据,表明多菌灵既不存在致突变活性,也存在致突变活性。结果的差异可能是由于杂质的存在造成的。本研究的目的是比较评价多菌灵的各种技术产品的遗传毒性。材料和方法。采用平板掺入Ames试验研究了多菌灵对5株鼠伤寒沙门菌在存在和不存在代谢激活系统(+S9/-S9)及CD-1小鼠微核试验条件下的遗传毒性。对多菌灵的两种技术级有效成分(TGAI)和一种分析标准进行了检测。结果。在Ames试验中,多菌灵分析标准品对所有菌株均无致突变活性(±S9)。TA98菌株对TGAI - 1的诱变效果最为显著,最大浓度下的应答数是阴性对照的5-7倍。多菌灵TGAIs在Ames试验中的积极作用可能是由杂质的存在介导的。在体内条件下,所有测试的多菌灵TGAIs诱导小鼠骨髓多染红细胞(PCE)微核的形成具有统计学意义和剂量依赖性。最大剂量下微核PCE的平均发生频率是阴性对照的21-24倍。研究的局限性。本研究仅限于对两种多菌灵技术产品样品和一种多菌灵分析标准样品的体内和体外诱变活性进行测试。结论。考虑到被测TGAIs中活性物质的高含量,细菌反向突变试验是评价多菌灵仿制产品等效性的高灵敏度方法。由于明显的非优生效应,使用微核试验来评估多菌灵TGAIs与原物质的等效性是不合适的。
{"title":"Comparative evaluation of the genotoxicity of carbendazim technical grade active ingredients in the Ames test and micronucleus in vivo test","authors":"O. Egorova, N. S. Averyanova, L. A. Kara, Natalya Alexandrovna Ilyushina","doi":"10.47470/0869-7922-2022-30-5-277-285","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-277-285","url":null,"abstract":"Introduction. Carbendazim is a systemic benzimidazole fungicide used against a wide range of crop diseases. The ability of carbendazim to induce the incidence of chromosomal aberrations and micronuclei in mammalian cells by influencing the processes of mitotic spindle formation in the cell cycle have been shown in various in vitro and in vivo tests. Contradictory data were obtained in the bacterial test system Salmonella/microsomes, indicating both the absence and the presence of mutagenic activity of carbendazim. The discrepancy in the results may stem from the presence of impurities. The aim of the study was a comparative evaluation of the genotoxicity of various technical products of carbendazim. Materials and methods. The genotoxicity of carbendazim was studied using the plate incorporation version of the Ames test on 5 strains of Salmonella typhimurium in the presence and the absence of metabolic activation system (+S9/-S9) and in a micronucleus test in CD-1 mice. Two technical grade active ingredients (TGAI) and an analytical standard for carbendazim were tested. Results. In the Ames test, the analytical standard of carbendazim possessed no mutagenic activity (±S9) on any of the strains. The most pronounced mutagenic effect was observed for the TGAI I in TA98 strain, the number of revertants at the maximum concentration was 5-7 times higher than that in the negative control. The positive effects of carbendazim TGAIs in the Ames test are likely mediated by the presence of impurities. Under in vivo conditions, all tested TGAIs of carbendazim induced a statistically significant and dose-dependent formation of micronuclei in polychromatic erythrocytes (PCE) of mouse bone marrow. The mean frequency of PCE with micronuclei at the maximum dose exceeded this rate in the negative control by 21-24 times. Research limitations. The study is limited to testing the mutagenic activity of two samples of carbendazim technical products and one sample of its analytical standard in both in vivo and in vitro tests. Conclusion. Taking into account the high content of the active substance in the tested TGAIs, the bacterial reverse mutation test is a highly sensitive method for assessment of the equivalence of carbendazim generic products. The use of a micronucleus test for evaluating of the equivalence of carbendazim TGAIs to the original substance is inappropriate due to the pronounced aneugenic effect.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88965213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicometabolomics — integration of preventive and analytical toxicology 毒物代谢组学-预防和分析毒理学的整合
Pub Date : 2022-10-30 DOI: 10.47470/0869-7922-2022-30-5-286-296
A. Radilov, A. Ukolov
Introduction. This paper considers aspects of the development of a toxicokinetically based model for scaling the limit concentrations of toxic compounds in the air, estimating the coefficients of their material cumulation, as well as estimating the concentrations of toxicants in biological media expected in the course of biological control. The results of testing the model on the example of organophosphorus pesticides (OP) and volatile industrial pollutants (VIP) are presented. Material and methods. For experimental modeling of intoxication, male chinchilla rabbits obtained from the Rappolovo nursery were used. Blood sampling was carried out from the marginal vein of the ear. For the highly sensitive determination of OP and VIP in biological samples, previously developed highly sensitive gas chromatographic techniques were used. Calculation of toxicokinetic parameters was made using a two-compartment model. Results. A toxicokinetically based model for scaling the limit concentrations of toxic compounds in the air, estimating the coefficients of their material cumulation, and estimating the concentrations of toxicants in biological media expected in the course of biological control is proposed. Research limitations. The proposed algorithm for scaling toxicokinetic parameters can be applied under the condition that the bioavailability of chemical compounds for the animal and human body is close, as well as the area under the toxicokinetic curve that is close to a linear dependence on the dose. Conclusion. Using the proposed toxicokinetically substantiated model for scaling the threshold concentrations, recommendations were made on chemical-analytical methods of biological control in the working area and atmospheric air for OP and VIP.
介绍。本文考虑了基于毒物动力学模型的发展的各个方面,该模型用于缩放空气中有毒化合物的极限浓度,估计其物质累积系数,以及估计生物控制过程中预期的生物介质中有毒物质的浓度。以有机磷农药(OP)和挥发性工业污染物(VIP)为例,给出了模型的检验结果。材料和方法。中毒实验模型选用Rappolovo苗圃获得的雄性栗鼠家兔。从耳缘静脉采血。对于生物样品中OP和VIP的高灵敏度测定,采用了先前开发的高灵敏度气相色谱技术。采用双室模型计算毒理动力学参数。结果。提出了一种基于毒物动力学的模型,用于计算空气中有毒化合物的极限浓度,估计其物质累积系数,以及估计生物控制过程中预期的生物介质中有毒物质的浓度。研究的局限性。本文提出的毒代动力学参数标度算法适用于化合物对动物和人体的生物利用度接近,以及毒代动力学曲线下的面积与剂量接近线性依赖的情况。结论。利用提出的毒物动力学模型来确定阈值浓度,对OP和VIP工作区域和大气中的生物控制化学分析方法提出了建议。
{"title":"Toxicometabolomics — integration of preventive and analytical toxicology","authors":"A. Radilov, A. Ukolov","doi":"10.47470/0869-7922-2022-30-5-286-296","DOIUrl":"https://doi.org/10.47470/0869-7922-2022-30-5-286-296","url":null,"abstract":"Introduction. This paper considers aspects of the development of a toxicokinetically based model for scaling the limit concentrations of toxic compounds in the air, estimating the coefficients of their material cumulation, as well as estimating the concentrations of toxicants in biological media expected in the course of biological control. The results of testing the model on the example of organophosphorus pesticides (OP) and volatile industrial pollutants (VIP) are presented. Material and methods. For experimental modeling of intoxication, male chinchilla rabbits obtained from the Rappolovo nursery were used. Blood sampling was carried out from the marginal vein of the ear. For the highly sensitive determination of OP and VIP in biological samples, previously developed highly sensitive gas chromatographic techniques were used. Calculation of toxicokinetic parameters was made using a two-compartment model. Results. A toxicokinetically based model for scaling the limit concentrations of toxic compounds in the air, estimating the coefficients of their material cumulation, and estimating the concentrations of toxicants in biological media expected in the course of biological control is proposed. Research limitations. The proposed algorithm for scaling toxicokinetic parameters can be applied under the condition that the bioavailability of chemical compounds for the animal and human body is close, as well as the area under the toxicokinetic curve that is close to a linear dependence on the dose. Conclusion. Using the proposed toxicokinetically substantiated model for scaling the threshold concentrations, recommendations were made on chemical-analytical methods of biological control in the working area and atmospheric air for OP and VIP.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83822727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
Toxicological Review
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1