Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-49-57
Anastasiya Yurevna Simonova, P. Rozhkov, Mariya Vladimirovna Belova, Kapitolina Konstsntinovna Ilyashenko, M. M. Potskhveriya, Y. Ostapenko, Y. S. Goldfarb, Anna Ivanovna Fetisova, A. N. Elkov, Tatyana Anatolevna Sokolova
Introduction. On March 11, 2020, WHO announced the global COVID-19 pandemic. According to literature data, the spread of coronavirus infection COVID-19 affected the structure of acute chemical poisoning. The aim. Assessment of the impact of the COVID-19 pandemic and restrictive measures on the peculiarities of seeking advice and the structure of acute chemical poisoning in Moscow. Material and methods. The data of the registration forms “Card of recording of a consultation of a patient with acute poisoning of chemical etiology” in the period from March 30 to June 30, 2020 and 2019 were studied. The analysis indicators of a hospitalization of patients in the department of acute poisoning and somatopsychiatric disorders of N.V. Sklifosovsky, the Research Institute of Emergency Medicine. Results. It was found that in the analyzed period of 2020 compared to 2019, the proportion of injured people of working age decreased by 11.2%, while it increased at the age of 0-17 years by 3.2 times. An increase in the number of consultations for individuals by 2.1 times was noted. There was an increase in the number of complaints about poisoning with antibiotics, drugs from the T45 group (anticoagulants, vitamins, etc.), T37 (antiviral, anti-microbial, etc.) by 1.7-2 times. In March-June 2020, there was an increase in the number of complaints about the toxic effects of alcohol (T51), an increase in hospitalized patients with acute alcohol poisoning by 4 times, and with combined alcohol poisoning - by 2.7 times. The number of complaints about poisoning with detergents and disinfectants in the first three months of the pandemic increased by 2.7 times. Conclusion. The pandemic and the introduction of restrictive measures for COVID-19 have affected the toxicological situation in Moscow, which must be taken into account when determining the guidelines for organizational measures for the provision of medical care for acute chemical poisoning during the COVID-19 pandemic.
{"title":"Analysis of the toxicological situation in Moscow in the first three months of the COVID-19 pandemic","authors":"Anastasiya Yurevna Simonova, P. Rozhkov, Mariya Vladimirovna Belova, Kapitolina Konstsntinovna Ilyashenko, M. M. Potskhveriya, Y. Ostapenko, Y. S. Goldfarb, Anna Ivanovna Fetisova, A. N. Elkov, Tatyana Anatolevna Sokolova","doi":"10.36946/0869-7922-2021-29-5-49-57","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-49-57","url":null,"abstract":"Introduction. On March 11, 2020, WHO announced the global COVID-19 pandemic. According to literature data, the spread of coronavirus infection COVID-19 affected the structure of acute chemical poisoning. The aim. Assessment of the impact of the COVID-19 pandemic and restrictive measures on the peculiarities of seeking advice and the structure of acute chemical poisoning in Moscow. Material and methods. The data of the registration forms “Card of recording of a consultation of a patient with acute poisoning of chemical etiology” in the period from March 30 to June 30, 2020 and 2019 were studied. The analysis indicators of a hospitalization of patients in the department of acute poisoning and somatopsychiatric disorders of N.V. Sklifosovsky, the Research Institute of Emergency Medicine. Results. It was found that in the analyzed period of 2020 compared to 2019, the proportion of injured people of working age decreased by 11.2%, while it increased at the age of 0-17 years by 3.2 times. An increase in the number of consultations for individuals by 2.1 times was noted. There was an increase in the number of complaints about poisoning with antibiotics, drugs from the T45 group (anticoagulants, vitamins, etc.), T37 (antiviral, anti-microbial, etc.) by 1.7-2 times. In March-June 2020, there was an increase in the number of complaints about the toxic effects of alcohol (T51), an increase in hospitalized patients with acute alcohol poisoning by 4 times, and with combined alcohol poisoning - by 2.7 times. The number of complaints about poisoning with detergents and disinfectants in the first three months of the pandemic increased by 2.7 times. Conclusion. The pandemic and the introduction of restrictive measures for COVID-19 have affected the toxicological situation in Moscow, which must be taken into account when determining the guidelines for organizational measures for the provision of medical care for acute chemical poisoning during the COVID-19 pandemic.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74999087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-34-42
B. Katsnelson, I. N. Chernyshov, S. Solovyeva, I. Minigalieva, V. Gurvich, I. E. Valamina, O. Makeyev, R. R. Sahautdinova, L. Privalova, A. E. Tsaregorodtseva, A. V. Korotkov, E. Shuman, V. Panov, M. Sutunkova
Introduction. Nickel oxide nanoparticles are of interest for toxicological science, not only as engineered nanoparticles, producing for industrial and scientific needs, but also as spontaneous pollutants of the atmosphere and the working area in industrial processes related to metallurgy and welding. Materials and methods. Rats were exposed to nickel-oxide aerosol at a concentration of 2.4 ± 0.4 µg/m3 in a “nose only” inhalation setup for 4 hours at a time, 5 times a week, during an overall period of 2 weeks to 6 months. Results. Of the several dozen examined parameters, only a few statistically significant manifestations associated with the reaction of the deep airways to inhaled nanoparticles were noted. However, in the biochemical and morphometric parameters of the lungs, even at the longest periods of exposure, the intergroup differences were insignificant. At the same time, even from the first weeks of the exposure period, genotoxic and allergic indices shifts are detected. Conclusion. For most of the evaluated effects, this level of exposure to nickel oxide nanoparticles may be considered as close to LOAEL, or even to NOAEL. However, according to some indicators, there are effects that suggest a non-threshold nature.
{"title":"About the threshold concentration of nickel oxide nanoparticles in long-term inhalation exposure of rats","authors":"B. Katsnelson, I. N. Chernyshov, S. Solovyeva, I. Minigalieva, V. Gurvich, I. E. Valamina, O. Makeyev, R. R. Sahautdinova, L. Privalova, A. E. Tsaregorodtseva, A. V. Korotkov, E. Shuman, V. Panov, M. Sutunkova","doi":"10.36946/0869-7922-2021-29-5-34-42","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-34-42","url":null,"abstract":"Introduction. Nickel oxide nanoparticles are of interest for toxicological science, not only as engineered nanoparticles, producing for industrial and scientific needs, but also as spontaneous pollutants of the atmosphere and the working area in industrial processes related to metallurgy and welding. Materials and methods. Rats were exposed to nickel-oxide aerosol at a concentration of 2.4 ± 0.4 µg/m3 in a “nose only” inhalation setup for 4 hours at a time, 5 times a week, during an overall period of 2 weeks to 6 months. Results. Of the several dozen examined parameters, only a few statistically significant manifestations associated with the reaction of the deep airways to inhaled nanoparticles were noted. However, in the biochemical and morphometric parameters of the lungs, even at the longest periods of exposure, the intergroup differences were insignificant. At the same time, even from the first weeks of the exposure period, genotoxic and allergic indices shifts are detected. Conclusion. For most of the evaluated effects, this level of exposure to nickel oxide nanoparticles may be considered as close to LOAEL, or even to NOAEL. However, according to some indicators, there are effects that suggest a non-threshold nature.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"232 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77009007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-58-63
Susanna B Poghosyan, Natalya S Tadevosyan, Susanna A Muradyan, Siranush Hovhannes Ter-Zakaryan, Ashot Norayr Jzhandzhapanyan, G. Kirakosyan
Introduction. Herbal medicines are unique therapeutic agents that represent multicomponent complexes of biologically active substances. They have low toxicity, a wide spectrum of therapeutic action with a minimum of side effects and a relatively low cost. Capparis spinosa L. has been widely used in traditional medicine for the treatment of various diseases and health conditions. Material and methods. An assessment of the toxicometric parameters of Capparis spinosa L. seed’s oil was done by a single oral and dermal exposure, the average lethal doses have been calculated for both routes, sensitivity by sex has been studied. The local irritant, skin resorptive, effect on the mucous membranes of the eyes of experimental animals was investigated. Sensitizing activity was studied according to the complex sensitization scheme. Processing of the results was carried out by the method of variation statistics. Standard errors and other indicators were calculated using the Litchfield-Wilcoxon probabilistic analysis method modified by Prozorovsky. The difference in mean values was estimated using Student’s t-test. Results. The acute toxicity of the oil was not expressed, death of animals was not observed. Average lethal dose for a single oral exposure was above 7000 mg/kg and dermal more than 3000 mg/kg. Sensitivity by sex in an acute experiment has not been established. The oil had a mild irritating effect on the mucous membranes of the eyes. It did not show skin-irritating, resorptive-toxic and sensitizing effects. Conclusion. Capparis spinosa L seed’s oil belongs to the 4th hazard class (low hazard) according to the hygienic classification and safety requirements of substances.
{"title":"Toxicological assessment of Capparis spinosa L. seed’s oil","authors":"Susanna B Poghosyan, Natalya S Tadevosyan, Susanna A Muradyan, Siranush Hovhannes Ter-Zakaryan, Ashot Norayr Jzhandzhapanyan, G. Kirakosyan","doi":"10.36946/0869-7922-2021-29-5-58-63","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-58-63","url":null,"abstract":"Introduction. Herbal medicines are unique therapeutic agents that represent multicomponent complexes of biologically active substances. They have low toxicity, a wide spectrum of therapeutic action with a minimum of side effects and a relatively low cost. Capparis spinosa L. has been widely used in traditional medicine for the treatment of various diseases and health conditions. Material and methods. An assessment of the toxicometric parameters of Capparis spinosa L. seed’s oil was done by a single oral and dermal exposure, the average lethal doses have been calculated for both routes, sensitivity by sex has been studied. The local irritant, skin resorptive, effect on the mucous membranes of the eyes of experimental animals was investigated. Sensitizing activity was studied according to the complex sensitization scheme. Processing of the results was carried out by the method of variation statistics. Standard errors and other indicators were calculated using the Litchfield-Wilcoxon probabilistic analysis method modified by Prozorovsky. The difference in mean values was estimated using Student’s t-test. Results. The acute toxicity of the oil was not expressed, death of animals was not observed. Average lethal dose for a single oral exposure was above 7000 mg/kg and dermal more than 3000 mg/kg. Sensitivity by sex in an acute experiment has not been established. The oil had a mild irritating effect on the mucous membranes of the eyes. It did not show skin-irritating, resorptive-toxic and sensitizing effects. Conclusion. Capparis spinosa L seed’s oil belongs to the 4th hazard class (low hazard) according to the hygienic classification and safety requirements of substances.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"180 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74510427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-4-16
A. I. Golovko, Jury Jurievich Ivnitsky, M. B. Ivanov, VL Rejnyuk
Introduction. The neurotoxic effect is considered as one of the variants of the toxicity of many xenobiotics. Neurotoxic effects develop not only in poisoning, but also when exposed to biological (for example, pathogens of infectious diseases) and physical (for example, ionizing and non-ionizing radiation) factors. Materials and methods. The subject of the analysis was the phenomenon of neurotoxicity. The information was obtained by studying the databases Scopus, Web of Science, PubMed, RSCI. Results. The absence of a single definition of the concept of «neurotoxicant» is noted. In addition to chemicals, other factors have neurotoxicity: biological, physical. The mechanisms of neurodegeneration under the influence of neurotoxicants with different mechanisms of action are similar and include excitotoxicity, neuroinflammation, suppression of mitochondrial function, inhibition of neurogenesis and gliogenesis, oxidative stress, increased BBB permeability and apoptosis. The presented features allow us to speak about the universality of the phenomenon of «neurotoxicity». Conclusion. When considering the phenomenon of «neurotoxicity», certain difficulties arise. A clear idea of the etiological factors of this phenomenon is not fully formulated. A comprehensive classification of neurotoxicants has not been created. At the same time, the processes of neurodegeneration are very similar in cases of poisoning with neurotoxicants with different mechanisms of action, which proves the universality of the phenomenon of «neurotoxicity».
介绍。神经毒性作用被认为是许多外源性药物毒性的变体之一。神经毒性不仅在中毒时产生，而且在暴露于生物(例如传染病病原体)和物理(例如电离和非电离辐射)因素时也会产生。材料和方法。分析的主题是神经毒性现象。研究结果来源于Scopus、Web of Science、PubMed、RSCI等数据库。结果。注意到缺乏“神经毒物”概念的单一定义。除了化学物质，其他因素也有神经毒性:生物的、物理的。不同作用机制的神经毒物影响下的神经变性机制相似，包括兴奋性毒性、神经炎症、线粒体功能抑制、神经发生和胶质发生抑制、氧化应激、血脑屏障通透性增加和细胞凋亡。所呈现的特征使我们能够谈论“神经毒性”现象的普遍性。结论。在考虑“神经毒性”现象时，出现了某些困难。对这一现象的病因还没有完全明确的认识。神经毒物的全面分类尚未形成。与此同时，不同作用机制的神经毒物中毒的神经变性过程非常相似，这证明了“神经毒性”现象的普遍性。
{"title":"Universality of the phenomenon of «neurotoxicity» (literature review)","authors":"A. I. Golovko, Jury Jurievich Ivnitsky, M. B. Ivanov, VL Rejnyuk","doi":"10.36946/0869-7922-2021-29-5-4-16","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-4-16","url":null,"abstract":"Introduction. The neurotoxic effect is considered as one of the variants of the toxicity of many xenobiotics. Neurotoxic effects develop not only in poisoning, but also when exposed to biological (for example, pathogens of infectious diseases) and physical (for example, ionizing and non-ionizing radiation) factors. Materials and methods. The subject of the analysis was the phenomenon of neurotoxicity. The information was obtained by studying the databases Scopus, Web of Science, PubMed, RSCI. Results. The absence of a single definition of the concept of «neurotoxicant» is noted. In addition to chemicals, other factors have neurotoxicity: biological, physical. The mechanisms of neurodegeneration under the influence of neurotoxicants with different mechanisms of action are similar and include excitotoxicity, neuroinflammation, suppression of mitochondrial function, inhibition of neurogenesis and gliogenesis, oxidative stress, increased BBB permeability and apoptosis. The presented features allow us to speak about the universality of the phenomenon of «neurotoxicity». Conclusion. When considering the phenomenon of «neurotoxicity», certain difficulties arise. A clear idea of the etiological factors of this phenomenon is not fully formulated. A comprehensive classification of neurotoxicants has not been created. At the same time, the processes of neurodegeneration are very similar in cases of poisoning with neurotoxicants with different mechanisms of action, which proves the universality of the phenomenon of «neurotoxicity».","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"126 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87673359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-17-24
A. Grebenyuk, V. N. Bykov
Introduction. Carbon monoxide (CO) is one of the most common causes of chemical injuries and the main toxic factor in the people death in fires. The mechanism of the toxic effect of CO, associated with the formation of carboxyhemoglobin and the development of hypoxia, determines the rapid development of the clinical picture of acute intoxication and the need for emergency first aid and medical care to the poisoned. Material and methods. Literature sources, summarized in the bibliographic databases eLIBRARY.RU, PubMed and Scopus, were the material for analysis. Results. First aid is based on quickly removing the victim from the fire zone or other area with a high concentration of CO and providing him with oxygen as soon as possible. During medical evacuation, it is necessary to carry out continuous inhalation of 80-100% oxygen, to ensure rest and warmth of the victim. In the emergency department of the hospital, oxygen inhalation and maintenance therapy should be continued, clinical and laboratory diagnostic measures aimed at assessing the severity of intoxication, identifying complications and concomitant pathology should be performed. In the case of severe CO poisoning, medical care continues to be provided to the victims in the intensive care unit or in the oxygen-barotherapy unit. The main antidote for CO poisoning is oxygen, which can be used in two versions - normobaric or hyperbaric oxygenation. As a pharmacological antidote to CO, zinc bisvinylimidazole diacetate (acizol) which can accelerate the breakdown of carboxyhemoglobin, improve the oxygen-binding and gas-transport properties of blood, as well as the dissociation of oxyhemoglobin in tissues is used. Along with antidotes, an important role in the treatment of CO poisoning is played by pathogenetic and symptomatic therapy aimed at the prevention and treatment of toxic encephalopathy, brain edema, cognitive dysfunction, toxic myocardiodystrophy and arrhythmia, prevention of pneumonia, correction of the acid-base state, compensation of the energy needs of the body, etc. Conclusion. Further improvement of existing means and methods for the treatment of intoxication, the development and introduction of new antidots into medical practice will increase the effectiveness of therapeutic measures, reduce the number of deaths and disabilities after acute poisoning with carbon monoxide.
{"title":"Carbon monoxide: modern concepts to the treatment of acute poisonings (literature review)","authors":"A. Grebenyuk, V. N. Bykov","doi":"10.36946/0869-7922-2021-29-5-17-24","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-17-24","url":null,"abstract":"Introduction. Carbon monoxide (CO) is one of the most common causes of chemical injuries and the main toxic factor in the people death in fires. The mechanism of the toxic effect of CO, associated with the formation of carboxyhemoglobin and the development of hypoxia, determines the rapid development of the clinical picture of acute intoxication and the need for emergency first aid and medical care to the poisoned. Material and methods. Literature sources, summarized in the bibliographic databases eLIBRARY.RU, PubMed and Scopus, were the material for analysis. Results. First aid is based on quickly removing the victim from the fire zone or other area with a high concentration of CO and providing him with oxygen as soon as possible. During medical evacuation, it is necessary to carry out continuous inhalation of 80-100% oxygen, to ensure rest and warmth of the victim. In the emergency department of the hospital, oxygen inhalation and maintenance therapy should be continued, clinical and laboratory diagnostic measures aimed at assessing the severity of intoxication, identifying complications and concomitant pathology should be performed. In the case of severe CO poisoning, medical care continues to be provided to the victims in the intensive care unit or in the oxygen-barotherapy unit. The main antidote for CO poisoning is oxygen, which can be used in two versions - normobaric or hyperbaric oxygenation. As a pharmacological antidote to CO, zinc bisvinylimidazole diacetate (acizol) which can accelerate the breakdown of carboxyhemoglobin, improve the oxygen-binding and gas-transport properties of blood, as well as the dissociation of oxyhemoglobin in tissues is used. Along with antidotes, an important role in the treatment of CO poisoning is played by pathogenetic and symptomatic therapy aimed at the prevention and treatment of toxic encephalopathy, brain edema, cognitive dysfunction, toxic myocardiodystrophy and arrhythmia, prevention of pneumonia, correction of the acid-base state, compensation of the energy needs of the body, etc. Conclusion. Further improvement of existing means and methods for the treatment of intoxication, the development and introduction of new antidots into medical practice will increase the effectiveness of therapeutic measures, reduce the number of deaths and disabilities after acute poisoning with carbon monoxide.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"121 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78431708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-25-33
V. Vasilkevich, R. Bogdanov, K. S. Gilevskaya, Victoria Igorevna Kulikouskaya
Introduction. Nanocomposites synthesized by the “green chemistry” method do not contain toxic chemicals (reducing agents and organic solvents) as carriers and/or stabilizing shells. One of the representatives of this group of materials are nanocomposites based on silver, which are increasingly used in medical practice, veterinary medicine, and in some other fields. Material and methods. The nanocomposite is Ag0 nanoparticles coated with a highly methoxylated pectin shell. The concentration of Ag0 nanoparticles in the hydrosol of the pectin-Ag nanocomposite is 1.65 mmol/l, and the pectin content is 7.5 mg/ml. The size of the synthesized pectin-Ag nanocomposite is ~20-30 nm, more than 90% of the particles have a diameter of less than 20 nm, the value of the ξ-potential is 45.3 ± 0.7 mV. Toxicological studies were carried out on outbred rats. The main goal of the research was to study the toxic effects of the pectin-Ag nanocomposite in a subchronic experiment (90 days). At the end of the experiment, a complex of behavioral and clinical and laboratory parameters was determined, which made it possible to assess the biological effect of the nanocomposite on animals. The research results were statistically processed. Results. With subchronic intragastric administration of the pectin-Ag nanocomposite to laboratory animals (rats) for 3 months at doses of 50, 500, and 5000 mg/kg, it was found that the nanocomposite exhibits a dose-dependent general toxic effect with critical target organs - the liver and spleen and the main biochemical markers of toxicity effect - aminotransferase, alkaline phosphatase and lactate dehydrogenase. Conclusion. Experimental studies have made it possible to substantiate the threshold doses of the hydrosol of the pectin-Ag nanocomposite for the intragastric route of intake.
{"title":"Study of toxicity and peculiarities of biological effects of nanocomposite pectin-Ag: results of a subchronic experiment","authors":"V. Vasilkevich, R. Bogdanov, K. S. Gilevskaya, Victoria Igorevna Kulikouskaya","doi":"10.36946/0869-7922-2021-29-5-25-33","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-25-33","url":null,"abstract":"Introduction. Nanocomposites synthesized by the “green chemistry” method do not contain toxic chemicals (reducing agents and organic solvents) as carriers and/or stabilizing shells. One of the representatives of this group of materials are nanocomposites based on silver, which are increasingly used in medical practice, veterinary medicine, and in some other fields. Material and methods. The nanocomposite is Ag0 nanoparticles coated with a highly methoxylated pectin shell. The concentration of Ag0 nanoparticles in the hydrosol of the pectin-Ag nanocomposite is 1.65 mmol/l, and the pectin content is 7.5 mg/ml. The size of the synthesized pectin-Ag nanocomposite is ~20-30 nm, more than 90% of the particles have a diameter of less than 20 nm, the value of the ξ-potential is 45.3 ± 0.7 mV. Toxicological studies were carried out on outbred rats. The main goal of the research was to study the toxic effects of the pectin-Ag nanocomposite in a subchronic experiment (90 days). At the end of the experiment, a complex of behavioral and clinical and laboratory parameters was determined, which made it possible to assess the biological effect of the nanocomposite on animals. The research results were statistically processed. Results. With subchronic intragastric administration of the pectin-Ag nanocomposite to laboratory animals (rats) for 3 months at doses of 50, 500, and 5000 mg/kg, it was found that the nanocomposite exhibits a dose-dependent general toxic effect with critical target organs - the liver and spleen and the main biochemical markers of toxicity effect - aminotransferase, alkaline phosphatase and lactate dehydrogenase. Conclusion. Experimental studies have made it possible to substantiate the threshold doses of the hydrosol of the pectin-Ag nanocomposite for the intragastric route of intake.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"205 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76962346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-30DOI: 10.36946/0869-7922-2021-29-5-43-48
G. V. Kuklina, D. Pechenkin, S. S. Ipatov, Andrei Valentinovich Eremkin, Aleksei Aleksandrovich Kytmanov, O. V. Tikhvinskaya
Introduction. The aim of the work was development of enzyme immunoassay for detecting I and II types of shiga-like toxins and assessment of it diagnostic properties. Materials and methods. For the research, we used hybridomas producing monoclonal antibodies to shiga-like toxins of types I and II, obtained at the branch of the Federal State Budgetary Institution “48 Central Research Institute” of the Ministry of Defense of Russian Federation (Kirov); BALB/c mice; shiga-like toxins of types I and II. Hybridoma cells were cultured in culture flasks and in the peritoneal cavity of BALB/c mice. Monoclonal antibodies were isolated from ascitic fluids by precipitation with a saturated solution of ammonium sulfate, followed by purification by ion exchange chromatography. The obtained preparations of monoclonal antibodies were used to develop enzyme immunoassay for the detection of shiga-like toxins of types I and II. Specific components of enzyme immunoassay were freeze-dried in a protective environment. Results. As a result of research, preparative quantities of monoclonal antibodies against I and II types of shiga-like toxins were obtained and purified; selection of monoclonal antibodies for sorption on the solid phase and for the synthesis of immunoperoxidase conjugates was carried out. Conclusion. experimental enzyme immunoassay allowing to identify 1 ng/ml I and II types of shiga-like toxins in «sandwich»-ELISA was developed.
{"title":"Development of enzyme immunoassay for detecting I and II types of shiga-like toxins","authors":"G. V. Kuklina, D. Pechenkin, S. S. Ipatov, Andrei Valentinovich Eremkin, Aleksei Aleksandrovich Kytmanov, O. V. Tikhvinskaya","doi":"10.36946/0869-7922-2021-29-5-43-48","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-5-43-48","url":null,"abstract":"Introduction. The aim of the work was development of enzyme immunoassay for detecting I and II types of shiga-like toxins and assessment of it diagnostic properties. Materials and methods. For the research, we used hybridomas producing monoclonal antibodies to shiga-like toxins of types I and II, obtained at the branch of the Federal State Budgetary Institution “48 Central Research Institute” of the Ministry of Defense of Russian Federation (Kirov); BALB/c mice; shiga-like toxins of types I and II. Hybridoma cells were cultured in culture flasks and in the peritoneal cavity of BALB/c mice. Monoclonal antibodies were isolated from ascitic fluids by precipitation with a saturated solution of ammonium sulfate, followed by purification by ion exchange chromatography. The obtained preparations of monoclonal antibodies were used to develop enzyme immunoassay for the detection of shiga-like toxins of types I and II. Specific components of enzyme immunoassay were freeze-dried in a protective environment. Results. As a result of research, preparative quantities of monoclonal antibodies against I and II types of shiga-like toxins were obtained and purified; selection of monoclonal antibodies for sorption on the solid phase and for the synthesis of immunoperoxidase conjugates was carried out. Conclusion. experimental enzyme immunoassay allowing to identify 1 ng/ml I and II types of shiga-like toxins in «sandwich»-ELISA was developed.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88147313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.36946/0869-7922-2021-29-4-4-13
O. Egorova
Introduction. The test for the induction of reverse gene mutations (Ames test, OECD* guideline No. 471) is one of the most popular methods for assessing mutagenicity due to its ease of execution and the ability to detect up to 70-80% of substances with carcinogenic activity. The experimental protocol requires a minimum amount of test substance and standard microbiological laboratory equipment. To obtain the primary data, several days from the start of the experiment are required. Despite the existence of publications devoted to the detailed description of the standard Ames test protocol, there is a gap affecting a number of aspects of the procedure for confirming the competence of a testing center using this method in its practice. Materials and methods. When preparing this article, we used the literature data published in domestic and foreign literature over the past 20 years concerning experimental approaches to the implementation of the Ames test. The literature search was carried out in the Scopus, Medline, Google Scholar, RSCI databases. Results. In the FBES “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing the method for assessing the bacterial reverse mutation has found application in assessing the safety of technical products of pesticides, their mixtures and preparative forms, as well as in the examination of equivalence. Testing laboratory center on the basis of the FBES “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing is accredited for compliance with the state standard GOST ISO / IEC 17025-2019 ”General requirements for the competence of testing and calibration laboratories.“ In this article, based on new published data and our own practical experience, a number of necessary conditions are considered for demonstrating the competence of a testing laboratory using the test for the induction of reverse gene mutations in its practice, its ability to obtain reliable results and take actions to manage the risks of laboratory activities. The main attention is paid to ensuring such parameters of the test quality as indicator cultures, metabolic activation system, control of the background of spontaneous mutation, etc. Conclusion. The discussed practical issues can be useful for specialists from research laboratories planning to introduce this method into practice.
介绍。诱导反向基因突变试验(Ames试验，OECD*指南第471号)是评估致突变性最常用的方法之一，因为它易于执行，并且能够检测到高达70-80%具有致癌活性的物质。实验方案需要最少量的测试物质和标准微生物实验室设备。为了获得原始数据，从实验开始需要几天的时间。尽管已有出版物专门对标准Ames测试方案进行了详细描述，但在确认测试中心在实践中使用该方法的能力的程序的许多方面存在差距。材料和方法。在准备本文时，我们参考了近20年来国内外发表的有关Ames检验实施的实验方法的文献资料。文献检索在Scopus、Medline、Google Scholar、RSCI数据库中进行。结果。在联邦消费者权利保护和人类福利监督局的FBES“以F.F. Erisman命名的联邦卫生科学中心”中，评估细菌反向突变的方法已应用于评估农药技术产品、其混合物和制剂形式的安全性以及等效性检查。以联邦消费者权益保护和人类福利监督局FBES“以F.F. Erisman命名的联邦卫生科学中心”为基础的测试实验室中心，通过了国家标准GOST ISO / IEC 17025-2019“测试和校准实验室能力的一般要求”的认证。“在本文中，基于新发表的数据和我们自己的实践经验，考虑了一些必要条件，以证明测试实验室在实践中使用诱导反向基因突变的测试的能力，其获得可靠结果的能力以及采取措施管理实验室活动风险的能力。”主要关注指标培养物、代谢激活系统、自发突变背景控制等检测质量参数的保证。结论。所讨论的实际问题对于计划将该方法引入实践的研究实验室的专家来说是有用的。
{"title":"To the procedure of confirmation of the laboratory’s competence in performing mutagenicity assessment using the Ames test","authors":"O. Egorova","doi":"10.36946/0869-7922-2021-29-4-4-13","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-4-4-13","url":null,"abstract":"Introduction. The test for the induction of reverse gene mutations (Ames test, OECD* guideline No. 471) is one of the most popular methods for assessing mutagenicity due to its ease of execution and the ability to detect up to 70-80% of substances with carcinogenic activity. The experimental protocol requires a minimum amount of test substance and standard microbiological laboratory equipment. To obtain the primary data, several days from the start of the experiment are required. Despite the existence of publications devoted to the detailed description of the standard Ames test protocol, there is a gap affecting a number of aspects of the procedure for confirming the competence of a testing center using this method in its practice. Materials and methods. When preparing this article, we used the literature data published in domestic and foreign literature over the past 20 years concerning experimental approaches to the implementation of the Ames test. The literature search was carried out in the Scopus, Medline, Google Scholar, RSCI databases. Results. In the FBES “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing the method for assessing the bacterial reverse mutation has found application in assessing the safety of technical products of pesticides, their mixtures and preparative forms, as well as in the examination of equivalence. Testing laboratory center on the basis of the FBES “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing is accredited for compliance with the state standard GOST ISO / IEC 17025-2019 ”General requirements for the competence of testing and calibration laboratories.“ In this article, based on new published data and our own practical experience, a number of necessary conditions are considered for demonstrating the competence of a testing laboratory using the test for the induction of reverse gene mutations in its practice, its ability to obtain reliable results and take actions to manage the risks of laboratory activities. The main attention is paid to ensuring such parameters of the test quality as indicator cultures, metabolic activation system, control of the background of spontaneous mutation, etc. Conclusion. The discussed practical issues can be useful for specialists from research laboratories planning to introduce this method into practice.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86470742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.36946/0869-7922-2021-29-4-30-39
K. Khamidulina, E. Tarasova, A. S. Proskurina, E. Guseva
Introduction. In recent years, due to the stricter requirements for compliance with the provisions of the Rotterdam Convention, the volume of export notifications on the permission to import dangerous chemicals into the Russian Federation has increased significantly. Therefore, the purpose of our research was to analyze the nomenclature, volume, toxicity and danger of industrial chemicals and pesticides entering the Russian market, despite prohibitions or restrictions on their use at the international and national levels. Materials and methods. The materials used are applications for the import of chemical products (export notifications) of the National Designated authorities of the European Union, China, Great Britain, Serbia to the Russian Register of Potentially Dangerous Chemical and Biological Substances-a branch of the Federal State Scientific Research Center named after F.F. Erisman - in the period from 2018 to 2020. The year 2019 was considered in the most detail. Results and discussion. The analysis of applications showed an ever-increasing number of chemical supplies; for example, 130 notifications were received in 2018, 539 in 2019, and 565 in 2020. In 2019, the number of tin compounds prevailed in the nomenclature of imported products to the territory of the Russian Federation. As for the substances from Annex III of the Rotterdam Convention, most of the imported chemicals by tonnage were substances included in this document - 1,2-dibromoethane (EDB), carbofuran, ethylene oxide, ethylene dichloride (1,2-dichloroethane), which are imported for industrial use, and not for use as pesticides, as they are declared in the convention. Chemicals and pesticides imported into the country under the PIC procedure, with the exception of numerous derivatives of dibutyl - and dioctyltin, creosote, have an established hygienic standard in at least one human habitat. In most cases, they are assigned to hazard classes 1 and 2 according to the maximum permissible concentration. Conclusion. Chemical products that are highly dangerous for human health and the environment, which are banned or severely restricted in many states, caused specific and long-term effects on the body, and having high toxicity to natural biota are used in the Russian Federation economy. In this regard, there is a need to develop regulatory decisions at the national level and within the framework of the Eurasian Economic Commission on the prohibition or restriction of the circulation of substances and pesticides characterized by an unacceptable risk of exposure.
{"title":"Analysis of banned or severely restricted chemicals and pesticides entering the Russian market under the Rotterdam Convention on the prior informed consent procedure for certain hazardous chemicals and pesticides in international trade","authors":"K. Khamidulina, E. Tarasova, A. S. Proskurina, E. Guseva","doi":"10.36946/0869-7922-2021-29-4-30-39","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-4-30-39","url":null,"abstract":"Introduction. In recent years, due to the stricter requirements for compliance with the provisions of the Rotterdam Convention, the volume of export notifications on the permission to import dangerous chemicals into the Russian Federation has increased significantly. Therefore, the purpose of our research was to analyze the nomenclature, volume, toxicity and danger of industrial chemicals and pesticides entering the Russian market, despite prohibitions or restrictions on their use at the international and national levels. Materials and methods. The materials used are applications for the import of chemical products (export notifications) of the National Designated authorities of the European Union, China, Great Britain, Serbia to the Russian Register of Potentially Dangerous Chemical and Biological Substances-a branch of the Federal State Scientific Research Center named after F.F. Erisman - in the period from 2018 to 2020. The year 2019 was considered in the most detail. Results and discussion. The analysis of applications showed an ever-increasing number of chemical supplies; for example, 130 notifications were received in 2018, 539 in 2019, and 565 in 2020. In 2019, the number of tin compounds prevailed in the nomenclature of imported products to the territory of the Russian Federation. As for the substances from Annex III of the Rotterdam Convention, most of the imported chemicals by tonnage were substances included in this document - 1,2-dibromoethane (EDB), carbofuran, ethylene oxide, ethylene dichloride (1,2-dichloroethane), which are imported for industrial use, and not for use as pesticides, as they are declared in the convention. Chemicals and pesticides imported into the country under the PIC procedure, with the exception of numerous derivatives of dibutyl - and dioctyltin, creosote, have an established hygienic standard in at least one human habitat. In most cases, they are assigned to hazard classes 1 and 2 according to the maximum permissible concentration. Conclusion. Chemical products that are highly dangerous for human health and the environment, which are banned or severely restricted in many states, caused specific and long-term effects on the body, and having high toxicity to natural biota are used in the Russian Federation economy. In this regard, there is a need to develop regulatory decisions at the national level and within the framework of the Eurasian Economic Commission on the prohibition or restriction of the circulation of substances and pesticides characterized by an unacceptable risk of exposure.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74966821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.36946/0869-7922-2021-29-4-45-50
M. S. Grechina, Alena V. Suslova, Viktoriya V. Belitskaya
Introduction. In the direction of normative and methodological assurance of the safety of environmental objects and imported food products, analytical approaches to the determination of a fungicide of the quinone class - dithianone in the air and citrus fruits were optimized. Materials and methods. Measurements were performed by tandem liquid mass spectrometry with a triple quadrupole mass detector (HPLC-MS/MS) in multiple reaction monitoring (MRM) mode using electrostatic spray as the ionization source. The substance from the air was concentrated on Tenax TA sorption tubes based on a porous polymer sorbent, followed by extraction of dithianone from the tubes with acetone. The preparation of samples of citrus fruits was carried out by extraction of acidified acetonitrile (selected optimal pH 2) in the presence of salts of magnesium sulfate, sodium chloride and sodium citrate two- and three-substituted, followed by centrifugation and filtration of the extract through disposable syringe membrane filters (QuEChERS technology). Results. The developed techniques were tested on real samples using the pesticide in agricultural practice. The revealed levels of dithianon do not exceed the lower limit of quantitative determination: 0.000071 mg/m3 in ambient air (with an RISL value of 0.0001 mg/m3) and 0.01 mg/kg in citrus fruits (with an established MRL value of 3 mg/kg). Conclusion. The developed methods are approved as official documents and supplement the base of analytical methods in terms of control of atmospheric air and imported food products.
{"title":"On the issue of analytical control of quinone derivatives in habitats","authors":"M. S. Grechina, Alena V. Suslova, Viktoriya V. Belitskaya","doi":"10.36946/0869-7922-2021-29-4-45-50","DOIUrl":"https://doi.org/10.36946/0869-7922-2021-29-4-45-50","url":null,"abstract":"Introduction. In the direction of normative and methodological assurance of the safety of environmental objects and imported food products, analytical approaches to the determination of a fungicide of the quinone class - dithianone in the air and citrus fruits were optimized. Materials and methods. Measurements were performed by tandem liquid mass spectrometry with a triple quadrupole mass detector (HPLC-MS/MS) in multiple reaction monitoring (MRM) mode using electrostatic spray as the ionization source. The substance from the air was concentrated on Tenax TA sorption tubes based on a porous polymer sorbent, followed by extraction of dithianone from the tubes with acetone. The preparation of samples of citrus fruits was carried out by extraction of acidified acetonitrile (selected optimal pH 2) in the presence of salts of magnesium sulfate, sodium chloride and sodium citrate two- and three-substituted, followed by centrifugation and filtration of the extract through disposable syringe membrane filters (QuEChERS technology). Results. The developed techniques were tested on real samples using the pesticide in agricultural practice. The revealed levels of dithianon do not exceed the lower limit of quantitative determination: 0.000071 mg/m3 in ambient air (with an RISL value of 0.0001 mg/m3) and 0.01 mg/kg in citrus fruits (with an established MRL value of 3 mg/kg). Conclusion. The developed methods are approved as official documents and supplement the base of analytical methods in terms of control of atmospheric air and imported food products.","PeriodicalId":23128,"journal":{"name":"Toxicological Review","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88294971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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