Theriogenology最新文献

{"title":"Beta-aminoisobutyric acid improves bovine oocyte maturation and subsequent embryonic development by promoting lipid catabolism","authors":"Canqiang Lu ,&nbsp;Zhengda Li ,&nbsp;Fan Xia ,&nbsp;Ruru Jia ,&nbsp;Yun Wang ,&nbsp;Yuwei Bai ,&nbsp;Chunye Wei ,&nbsp;Yanyu Chen ,&nbsp;Mingzhe Lu ,&nbsp;Deshun Shi ,&nbsp;Fenghua Lu","doi":"10.1016/j.theriogenology.2024.12.016","DOIUrl":"10.1016/j.theriogenology.2024.12.016","url":null,"abstract":"<div><div>Energy metabolism homeostasis is essential for oocyte maturation and acquisition of developmental capacity. However, bovine oocyte <em>in vitro</em> maturation (IVM) is highly susceptible to metabolic stress and lipid accumulation. β-Aminoisobutyric acid (BAIBA), a metabolite produced in response to skeletal muscle exercise, has been reported to be involved in lipid and glucose metabolism, as well as inflammation and oxidative stress. This work aimed to evaluate the potential effects of BAIBA on bovine oocyte IVM and its mechanisms. Different concentrations of BAIBA (10, 20, 50, 100, and 200 μmol/L) were supplemented to bovine oocyte IVM medium. Results shown the BAIBA (50 μmol/L) had no effect on the extrusion rate of the first polar body of oocytes but significantly improved the subsequent blastocyst formation rate and embryo quality. Further revealed that supplementing BAIBA significantly up-regulated expression levels of genes to fatty acid β-oxidation metabolism (<em>CPT1A</em>, <em>CPT1B</em> and <em>CPT2</em>), promoted lipid metabolism, lowered lipid content, and improved mitochondrial membrane potential and active mitochondria content. Importantly, BAIBA stimulation significantly increased the phosphorylation of AMP-activated protein kinase (AMPK); and the inhibition of AMPK activity (Compound C, AMPK inhibitor) suppressed the ability of BAIBA to promote lipid metabolism in oocytes. Besides, inhibition of AMPK lowered the oocyte maturation rate and the subsequent zygote cleavage and blastocyst formation rate when compared to that of the BAIBA treatment. The results indicated that BAIBA was mainly involved in promoting lipid catabolism by activation of AMPK, consequently enhancing oocyte development potential.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 153-163"},"PeriodicalIF":2.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of BMP15 and GDF9 in the IVM medium on subsequent oocyte competence and embryo development of prepubertal goats","authors":"Mònica Ferrer-Roda,&nbsp;Maria-Teresa Paramio,&nbsp;Judith Vila-Beltrán,&nbsp;Dolors Izquierdo","doi":"10.1016/j.theriogenology.2024.12.015","DOIUrl":"10.1016/j.theriogenology.2024.12.015","url":null,"abstract":"<div><div>Oocyte-secreted factors (OSFs), such as BMP15 and GDF9, are soluble paracrine factors that drive cumulus cell differentiation and function, sustaining oocyte competence acquisition and embryo development. This study aimed to assess the effect of BMP15 and GDF9 on IVM medium of prepubertal goat oocytes. COCs were in vitro matured in absence (control group) or presence of 100 ng/mL of BMP15, GDF9, or both. To determine cumulus-oocyte communication, transzonal projections (TZP) density at 0h, 6h, 12h and 24h of IVM were evaluated. After IVM, mitochondrial activity, intracellular ROS and glutathione (GSH) levels, the epidermal growth factor receptor (EGFR) expression in oocytes and cumulus cells, and cumulus expansion were assessed. Blastocyst production and quality were evaluated after parthenogenetic activation (PA) and IVF. IVM supplementation with BMP15 increased the TZP density during the first 6 h of culture. After IVM, BMP15 increased mitochondrial activity, EGFR expression in oocytes and cumulus cells, and cumulus expansion compared to control, but ROS and GSH levels were similar to control. BMP15 improved blastocyst production following PA (15.5 % vs 6.3 %) and the number of cells in the blastocyst inner cell mass. No differences were observed on blastocyst production or quality following IVF. IVM supplementation with GDF9 did not improve results from control group in any parameters studied. Additionally, GDF9 in combination with BMP15 only improved mitochondrial activity and cumulus expansion over control. In conclusion, IVM medium supplementation with BMP15 (100 ng/ml) improves COCs quality parameters and PA-blastocyst production and quality of prepubertal goat oocytes. However, GDF9 (100 ng/mL) did not have any beneficial effect in this study and was possibly antagonistic to BMP15.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 164-173"},"PeriodicalIF":2.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial content and mtDNA copy number in spermatozoa and penetrability into oocytes","authors":"Hai Thanh Nguyen ,&nbsp;Son Quang Do ,&nbsp;Takuya Wakai ,&nbsp;Hiroaki Funahashi","doi":"10.1016/j.theriogenology.2024.12.012","DOIUrl":"10.1016/j.theriogenology.2024.12.012","url":null,"abstract":"<div><div>The current narrative review aims to summarize the relation of mitochondrial content (MC) and mitochondrial DNA copy number (MDCN) in spermatozoa with sperm penetrability, and to discuss the various determining factors during the process of spermatogenesis in mammals. There are many potential factors associated with the quantitative alteration of MC and MDCN in male gametes from spermatogenesis to ejaculation. Particularly, spermatogenesis may be the first step to jointly contribute to an incomplete reduction of MC and MDCN in spermatozoon. It appears to be now quite clear that some abnormalities during spermatogenesis and oxidative stress are the main factors highly associated with the quantitative change of MC and MDCN in spermatozoa, consequently affecting sperm quality and their penetrability into oocytes. Currently, a series of proteins contributing to form sperm midpiece during spermatogenesis and cytoplasmic elimination during spermiation have been currently identified. The present review provides insight into how these factors interact with sperm MC and MDCN, and handholds to gain a better understanding of their roles. This review also highlights the uniqueness of normal fertile spermatozoa which have relatively lower MC and MDCN, but have mitochondria that function completely in multiple pivotal physiological pathways.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 125-132"},"PeriodicalIF":2.4,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celebrating a milestone and looking at the future of Theriogenology","authors":"Marc-Antoine Driancourt ,&nbsp;Pierre Comizzoli ,&nbsp;Leonardo F.C. Brito","doi":"10.1016/j.theriogenology.2024.12.013","DOIUrl":"10.1016/j.theriogenology.2024.12.013","url":null,"abstract":"<div><div>In 2024, Theriogenology turned 50! The purpose of this special issue is to emphasize the pivotal role of reproduction and fertility in improving the efficacy and sustainability of animal production in a range of environments (different production systems, different climates, or different consumer-related expectations). A first series of articles summarizes the possibilities and constraints linked to embryo production (in vivo and in vitro) as well as the potential of novel embryo technologies. A second series provides an overview of the future of production and reproduction for the next 20 years in different species and geographical areas. A last series aims to illustrate the diversity and quality of the original research recently published in Theriogenology. Please enjoy the special issue at <span><span>https://www.sciencedirect.com/special-issue/10JH4BB3RP7</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 151-152"},"PeriodicalIF":2.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian stimulation of Nelore calves and prepubertal heifers with a long-acting recombinant human FSH (corifollitropin-alpha) and subsequent ovum pick-up and in vitro embryo production outcomes","authors":"Rodrigo Moura ,&nbsp;Carlos Antonio Fernandes ,&nbsp;Luiz Gustavo Siqueira ,&nbsp;Ricardo Alamino Figueiredo ,&nbsp;Carlos Frederico Martins ,&nbsp;Maurício Antonio Peixer ,&nbsp;Marcelo Cunha Xavier ,&nbsp;Joao Henrique Viana","doi":"10.1016/j.theriogenology.2024.12.011","DOIUrl":"10.1016/j.theriogenology.2024.12.011","url":null,"abstract":"<div><div>The aim of this study was to evaluate the use of a long-acting recombinant human FSH (rhFSH, corifollitropin-alpha) to induce ovarian stimulation in Nelore breed (<em>Bos indicus</em>) calves and prepubertal heifers prior to ovum pick-up (OPU) for <em>in vitro</em> embryo production (IVEP). In Experiment 1, a dose-response trial was performed to determine the optimal dose of rhFSH, which was determined to be 10 μg. In Experiment 2, 6–7 mo old calves were randomly allocated to receive rhFSH either via sc (n = 5) or im (n = 5). Ovarian follicular development was monitored daily by transrectal ultrasonography for five days. There was no effect of route (<em>P</em> = 0.1348) nor route × time interaction (<em>P</em> = 0.8336) on follicle development. In Experiment 3, 7–8 mo old calves (n = 90) were randomly allocated into 5 groups: 1) Control: no ovarian stimulation; 2) rhFSH-96: 10 μg rhFSH sc followed by OPU 96h later; 3) rhFSH-120: 10 μg rhFSH sc and OPU 120h later; 4) eCG-96: 300 IU eCG im and OPU 96h later; and 5) eCG-120: 300 IU eCG im and OPU 120h later. Non-rhFSH treated Nelore mature cows (n = 10) were used as reference-controls for IVEP outcomes. Treatment with rhFSH increased the proportion of grade I cumulus-oocyte complexes (COC) collected by OPU at either timepoints (96 or 120h) compared with eCG or controls (<em>P</em> &lt; 0.0001). Blastocyst rate for rhFSH-120 calves was similar to mature cows (<em>P</em> &gt; 0.05). Treatment with rhFSH, however, increased the proportion of expanded COC and decreased the proportion of viable COC (<em>P</em> &lt; 0.0001) compared with eCG and controls. In Experiment 4, yearling heifers (n = 60) were treated or not (control group) with 10 μg rhFSH sc and OPU was performed 72 or 96h later. Heifers treated with rhFSH had a greater proportion of grade I COC (<em>P</em> = 0.0188) and blastocyst rate (<em>P</em> &lt; 0.0098) than controls, regardless of the interval used. These groups, however, yielded a lesser number of viable COC (<em>P</em> = 0.0264), resulting in a similar (<em>P</em> = 0.5869) number of embryos produced by donor per OPU compared with controls. Pregnancy rate after embryo transfer was also similar between controls and rhFSH groups (19.3 vs 25.0 %, <em>P</em> = 0.4142). In summary, treatment with a single sc injection of corifollitropin-alpha was effective to promote ovarian stimulation in calves prior to OPU. The potential benefits of stimulatory protocols using rhFSH, however, have been overshadowed by a decrease in the total number of viable COC recovered per donor, thus failing to increase the number of embryos produced per OPU.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 110-116"},"PeriodicalIF":2.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different concentrations of N-acetylcysteine on the sperm quality, antioxidant enzyme activity, and antioxidant gene expression of cryopreserved goat semen","authors":"Xiaodong Wang ,&nbsp;Jinhong Luo ,&nbsp;Maosheng Cao ,&nbsp;Yonghong Ju ,&nbsp;Qingmeng Long ,&nbsp;Rong Yang ,&nbsp;Quan Ji ,&nbsp;Guangbin Zhou ,&nbsp;Junwei Zhang ,&nbsp;Ruiyang Li ,&nbsp;Xiang Chen","doi":"10.1016/j.theriogenology.2024.12.009","DOIUrl":"10.1016/j.theriogenology.2024.12.009","url":null,"abstract":"<div><div>During cryopreservation, spermatozoa produce excess reactive oxygen species (ROS), which attack the plasma membrane, disrupt the physiological structure of the sperm, and ultimately decrease semen quality. This study investigated the effects of different N-acetylcysteine (NAC) concentrations on the cryopreservation of semen from Qianbei Ma goats. Semen samples were collected from five bucks with motility rates above 80 %. The treatment groups were diluted 20-fold in extenders containing 3 or 9 mM NAC and cryopreserved in liquid nitrogen, whereas the control group did not include NAC. After thawing, the sperm motility, antioxidant gene expression, enzyme activity, and cell structure were analysed. The NAC-treated groups showed improved post-thaw sperm motility. The 9 mM NAC group presented the highest catalase (CAT) and glutathione peroxidase activities, lowest ROS levels, and fewest apoptotic sperms. Moreover, the 3 mM NAC group presented the highest superoxide dismutase activity and L-cysteine levels and the lowest malondialdehyde levels. Additionally, sperm membrane integrity and mitochondrial membrane potential were significantly higher in the NAC-treated group than that in the control. Further analysis of antioxidant and apoptotic gene expression in the treated sperm revealed that the 9 mM NAC group presented significantly greater <em>CAT</em> and <em>GPX4</em> expression than the control and 3 mM NAC groups, whereas the apoptotic genes <em>BAX</em> and <em>Caspase3</em> were elevated in the control group compared to both the NAC groups. In summary, adding NAC to semen extenders enhanced antioxidant gene expression, increased enzyme activity, and improved post-thaw semen quality, with the 9 mM NAC treatment showing the optimal effects.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 101-109"},"PeriodicalIF":2.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presynchronization with a progesterone device and prostaglandin F2α enhances ovulatory response to first GnRH, estrus expression and tended to increase fertility in beef heifers submitted to a 5-day CO-Synch protocol","authors":"T. Flores ,&nbsp;J.M. Sánchez ,&nbsp;I. Lopez-Helguera ,&nbsp;E. Rojas Canadas","doi":"10.1016/j.theriogenology.2024.12.010","DOIUrl":"10.1016/j.theriogenology.2024.12.010","url":null,"abstract":"<div><div>The main objectives of the present study were to determine the effects of presynchronizing with a 1.0 g intravaginal progesterone device (IVPD) and prostaglandin F2α and to assess the effects of re-utilization of IVPD in a 2x2 factorial design, on the ovulatory response to first GnRH, ovarian status at different protocol stages, estrus expression and fertility in beef heifers submitted to a 5d-CO-Synch + Progesterone (P4) protocol. Beef heifers (n = 564) were assigned to 1 of 2 treatments at D-15: Pres5 (n = 283), where heifers received a (IVPD) for 5 days and administration of prostaglandin F2α (25 mg of dinoprost) at D-10; and Control (n = 281), where heifers received no treatment. At D-8, all heifers received 100 μg of GnRH (gonadorelin acetate) and were assigned to 1 of 2 IVPD-use treatments: new-IVPD (n = 279), where animals received a new IVPD for 5 days, and once-used IVPD (n = 285), where heifers received a once-used IVPD for 5 days (used previously for 5 days). On D-3, IVPD was removed and 50 mg of prostaglandin F2α was administered. All heifers were timed artificially inseminated (AI; D0) 62 h after IVPD removal concomitant with an administration of 100 μg of GnRH. Estrus detection patches were placed on heifers at D-3 and evaluated at the time of AI. In a subset of heifers (n = 278), transrectal ultrasonography of the ovaries was carried out at D-8 and D-3 to assess presence and diameter of CL and largest follicle diameter (LFD). A blood sample was collected on D-3 to determine serum P4 concentration. Heifers with a once-used IVPD had a greater (<em>P</em> = 0.01) pregnancy per AI (P/AI) than heifers treated with a new-IVPD [62.8 % (179/285) vs 51.2 % (143/279)]. There was a lower percentage of animals (<em>P</em> = 0.002) having a corpus luteum (CL) on D-8 in Pres5 group compared to Control [16.4 % (22/134) vs 69.4 % (100/144)]. Similarly, a greater percentage of Pres5 heifers had a dominant follicle on D-8 (<em>P</em> &lt; 0.0001) than Control heifers [97.7 % (131/134) vs 75.7 % (109/144)]. At D-3, Pres5 heifers had a greater ovulatory response to D-8 GnRH (<em>P</em> &lt; 0.0001) compared to Control animals [82.8 % (111/134) vs 49.3 % (71/144)]. In addition, Pres5 heifers had a greater estrus expression behavior [87.6 % (248/283) vs 72.9 % (205/281); <em>P</em> &lt; 0.0001] and tended to have (<em>P</em> = 0.10) a greater P/AI [61.8 % (175/283) vs 52.3 % (147/281)] than Control heifers. In conclusion, the tendency for a greater fertility observed in Pres5 heifers (∼10 %) justifies the extra animal handling required for presynchronization. A once-used IVPD represents a viable strategy to enhance P/AI and reduce the cost in beef heifers submitted to timed AI.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 117-124"},"PeriodicalIF":2.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoglycyrrhizin supplementation of frozen goat semen-extender improves post-thaw sperm quality and in vitro fertilization rates","authors":"Fuqin Liu ,&nbsp;Lingwei Sun ,&nbsp;Jiehuan Xu ,&nbsp;Mengqian He ,&nbsp;Caifeng Wu ,&nbsp;Haoxing Shen ,&nbsp;Huibin Zhu ,&nbsp;Feng Luo ,&nbsp;Jun Gao ,&nbsp;Jianjun Dai","doi":"10.1016/j.theriogenology.2024.12.008","DOIUrl":"10.1016/j.theriogenology.2024.12.008","url":null,"abstract":"<div><div>This study examined the effect of supplementation of freezing extender with isoglycyrrhizin (ISL), a natural antioxidant agent, on the quality and fertility potential of goat spermatozoa after cryopreservation. Forty ejaculates were collected from eight Chongming White rams and diluted with five concentrations of ISL: 0 (control group), 50, 100, 150, and 200 μg/mL. The quality, motility parameters, antioxidant properties, mRNA expression of antioxidant and ferroptosis genes, and ability to induce fertilization, were evaluated following freezing/thawing. Total motility and progressive motility were significantly increased in spermatozoa following the addition of 150 and 200 μg/mL ISL. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities were enhanced in all ISL-supplemented groups compared to controls. Adding ISL decreased the levels of reactive oxygen species (ROS), malondialdehyde (MDA) and nitric oxide (NO). mRNA levels of ferroptosis-related genes, voltage-dependent anion channel protein 2 (<em>VDAC2</em>), voltage-dependent anion channel protein 3 (<em>VDAC3</em>), protein 53 (<em>P53</em>), and nuclear factor erythroid 2-related factor 2 (<em>NRF2</em>) tended to decrease after adding ISL, whereas the levels of antioxidant genes glutathione peroxidase 4 (<em>GPX4</em>) and glutathione peroxidase 4 (<em>GPX5</em>), tended to increase. The best spermatozoa quality and the strongest antioxidant properties were obtained after adding 150 μg/mL ISL. Therefore, fresh semen, frozen semen, and frozen semen with 150 μg/mL ISL was used for <em>in vitro</em> fertilization of oocytes. The cleavage and blastocyst rates were significantly lower in frozen semen compared with fresh semen, whereas frozen semen containing 150 μg/mL ISL showed a significant increase in cleavage and blastocyst rates compared with frozen semen. In conclusion, ISL can be used as an antioxidant in goat semen cryodilution, and the addition of ISL can improve the quality and antioxidant properties of frozen and thawed spermatozoa. ISL can also protect the ability of spermatozoa to be fertilized and develop; 150 μg/mL ISL was the optimal concentration for addition.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 133-142"},"PeriodicalIF":2.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of PPARγ in the development of early sheep embryos in vitro","authors":"Hengbin Yu ,&nbsp;Chang Liu ,&nbsp;Yue Zhang,&nbsp;Zhenghang Li,&nbsp;Wenhui Pi,&nbsp;Guangdong Hu","doi":"10.1016/j.theriogenology.2024.12.007","DOIUrl":"10.1016/j.theriogenology.2024.12.007","url":null,"abstract":"<div><div>Lipid metabolism plays an important role in the regulation of early embryonic development in mammals. However, the effect of lipid metabolism mediated by peroxisome proliferator-activated receptor γ (PPARγ) on the early embryonic development of sheep remains unclear. In this study, rosiglitazone (RSG), a PPARγ activator, was added to the <em>in vitro</em> embryo culture (IVC) medium to regulate the continuous expression of PPARγ. This study aimed to evaluate PPARγ expression during early embryonic development in sheep as well as its effects on lipid deposition, reactive oxygen species (ROS) and glutathione (GSH) levels, apoptosis and lipid metabolism-related gene expression, and embryonic development. PPARγ was not detected at 2-cell, 4-cell, 8-cell, and morula stage, while widely expressed with obvious nuclear expression features in blastocysts. Notably, treatment with 5 μM RSG in sheep parthenogenetic activated (PA) embryos significantly increased the blastocyst rate, lipid content, and GSH levels, while decreasing ROS levels. Further analysis revealed that RSG treatment upregulated the expression levels of antioxidant genes (<em>SOD2</em> and <em>CAT</em>), anti-apoptotic gene (<em>BCL2</em>), and lipid metabolism-related genes (<em>SCD-1</em>, <em>CD36</em>, <em>PLIN2</em>, <em>FABP3</em><em>,</em> and <em>FABP4</em>). Taken together, these results suggest that PPARγ plays a vital role in promoting embryonic development by enhancing lipid metabolism and reducing oxidative stress.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 143-150"},"PeriodicalIF":2.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered expression of systemic and ovarian factors involved in the recruitment and activation of immune cells in bovine cystic ovarian disease","authors":"Lucas Etchevers ,&nbsp;Pablo Uriel Díaz ,&nbsp;María Sol Renna ,&nbsp;Ulises Sebastian Notaro ,&nbsp;Fernanda Mariel Rodriguez ,&nbsp;Hugo Hector Ortega ,&nbsp;Natalia Raquel Salvetti ,&nbsp;Ayelén Noelia Amweg","doi":"10.1016/j.theriogenology.2024.12.006","DOIUrl":"10.1016/j.theriogenology.2024.12.006","url":null,"abstract":"<div><div>Despite the involvement of both immune cells and soluble factors in the etiopathogenesis of cystic ovarian disease (COD), the precise interplay between these components in this reproductive condition remains unclear. Based on this, in the present study we aimed to evaluate the expression of key molecules involved both locally and systemically in the recruitment, infiltration and activation of immune cells in Holstein cows diagnosed with follicular cysts (N = 5). For this, animals from commercial dairy farms located in the milk-productive region of Santa Fe, Argentina were included after their diagnosis were confirmed by ultrasonographic examination and hormonal evaluation. Healthy animals in proestrus, after G6G-Ovsynch hormonal synchronization, were used as controls (N = 5). Protein expression of adhesion molecules, along with mRNA expression of chemokine receptors and cytokines were evaluated. In peripheral blood mononuclear cells, gene expression analysis of inflammatory markers showed that <em>TNF</em> and <em>TGFB1</em> mRNA expression was significantly lower in COD animals. Also, immunohistochemical analysis revealed that expression of vascular cell adhesion molecule 1 and platelet endothelial cell adhesion molecule was lower in endothelial cells of medullary blood vessels in the COD group. These changes support the hypothesis that alterations in the expression patterns of immune factors, both at systemic and local level, could contribute to the etiopathogenesis of this anovulatory condition.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"Pages 92-100"},"PeriodicalIF":2.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}