Tissue Engineering. Part B, Reviews最新文献

Despite antiretroviral therapy's success in human immunodeficiency virus (HIV) management, no cure or preventive vaccine exists; three-dimensional (3D) human tissue models-emerging from biomedical research, tissue engineering, and microfluidics-offer new potential, yet a scientometric analysis of their progress remains lacking. We reviewed the current status of three in vitro 3D models for HIV research: organoids, organ-on-a-chip, and 3D bioprinting. We conducted a bibliometric comparative analysis of 3D human tissue models in HIV research. A total of 852 documents published between 2014 and 2024 were retrieved and analyzed. We found that brain organoids, intestinal organoids, tonsil organoids, kidney organoids, and thymus and spleen organoids effectively support HIV infection and are widely used in in vitro HIV research. Organ-on-a-chip has been primarily used for rapid HIV detection, while 3D bioprinting models have been used in areas such as in vitro HIV detection and diagnosis. Our results showed that the yearly output of articles in 3D human tissue models for HIV has remained relatively stable over the past decade. European institutions impacted greatly on the scientific society of HIV research in 3D human tissue models. The hotspots of 3D human tissue models for HIV research expanded from antiretroviral therapy and molecular docking to 3D printing and organoids. This comparative study presented a unique perspective to understand the evolutive history and future trends of 3D human tissue models for HIV and emerging human-relevant in vitro organotypic models.

Tooth regeneration is an exciting frontier in regenerative medicine, yet comprehensive cross-disciplinary analysis of its research landscape remains limited. This study presents the bibliometric analysis, integrating data from Web of Science (WOS) and Scopus, to quantify publication dynamics, journal influence, thematic structure, and translational priorities. Following PRISMA guidelines, we conducted a comprehensive search using keywords related to tooth regeneration, dental tissue engineering, and regenerative dentistry. After systematic screening and quality assessment, 925 articles were analyzed using descriptive statistics to identify publication trends, the most active and cited journals, and VOSviewer co-occurrence analysis to visualize the thematic mapping. The analysis revealed robust field growth. Among the 395 journals that published articles, the top 10 contributed 20% of publications, with the Journal of Dental Research (n = 35) and the Journal of Endodontics (n = 31) leading in productivity. The journals Scientific Reports and Biomaterials achieved the highest Eigenfactor score, while the Science Translational Medicine demonstrated the greatest journal prestige (SJR = 6.722). Co-occurrence analysis identified 384 unique keywords, revealing the presence of four research clusters: Biomaterials and Advanced Scaffold Design; Cellular and Experimental Foundations; Clinical Endodontics and Periodontal Regeneration; Developmental Biology and Tooth Morphogenesis. Stem cell dynamics emphasizes three groups of stem cells: dental-derived cells, specialized cell types and non-dental derived cells. Our bibliometric analysis provides a comprehensive review of the tooth regeneration landscape. Thematic synthesis of stem cells led to an understanding of the field's current limitations, challenges, and cutting-edge trends. This manuscript represents the first dual-database bibliometric and visualization-driven analysis of tooth regeneration research. It quantifies global publication dynamics, highlights the pivotal contributions of leading journals, and delineates four critical thematic clusters: Biomaterials and Advanced Scaffold Design, Cellular and Experimental Foundations, Clinical Endodontics and Periodontal Regeneration, and Developmental Biology and Tooth Morphogenesis. By systematically mapping stem cell applications into dental-derived, specialized, and non-dental populations, this study provides a novel cellular framework for evaluating translational readiness. Furthermore, it underscores emerging frontiers such as 3D bioprinting, bioactive scaffolds, exosome-based therapies, and genetic modulation, while identifying persistent challenges in vascularization, innervation, enamel regeneration, and clinical scalability. Collectively, this analysis offers clinicians, researchers, and policymakers a strategic roadmap for advancing functional tooth regeneration from laboratory innovation to clinical application.

The repair and reconstruction of oral mucosal defects are critical for restoring both function and aesthetics of the oral cavity. Tissue engineering, which integrates principles from engineering and life sciences, has enabled the development of biological substitutes that closely mimic the native structure and function of oral mucosa, significantly reducing the risks and complications associated with autologous transplantation. With the rapid advancement of tissue-engineered oral mucosa (TEOM) technology, its applications in regenerative medicine and oral disease modeling have become increasingly prominent. In recent years, innovative strategies such as the development of organoids, prevascularization, immunomodulation, and dermal-epidermal junction biomimicry have emerged, providing effective solutions to challenges related to inadequate vascularization, immune dysregulation, and mechanical performance in TEOM constructs. In addition, the application of cutting-edge manufacturing technologies such as 3D bioprinting has accelerated the translation of TEOM toward clinical use. This review outlines the fundamental principles, design strategies, and potential applications of TEOM, and discusses novel approaches and challenges that must be addressed to facilitate its clinical implementation. Impact Statement This review provides a critical synthesis of recent advances in tissue-engineered oral mucosa, emphasizing cutting-edge methodologies in biomaterial development, cell engineering, and microenvironment modulation. By identifying unresolved challenges such as vascularization and immunomodulation, and proposing innovative strategies, including organoids and smart biomaterials, this article provides a valuable framework for researchers and clinicians striving to translate laboratory breakthroughs into effective regenerative therapies. This integrative perspective is poised to accelerate progress in oral mucosal repair across a variety of clinical applications.

Synthetic bone transplantation has emerged in recent years as a highly promising strategy to address the major clinical challenge of bone tissue defects. In this field, bioactive glasses (BGs) have been widely recognized as a viable alternative to traditional bone substitutes due to their unique advantages, including favorable biocompatibility, pronounced bioactivity, excellent biodegradability, and superior osseointegration properties. This article begins with a comprehensive overview of the development and success of BGs in bone tissue engineering, and then focuses on their composite reinforcement systems with biodegradable metals, calcium-phosphorus (Ca-P)-based bioceramics, and biodegradable medical polymers, respectively. Moreover, the article outlines some frequently used manufacturing methods for three-dimensional BG-based bone bioscaffolds and highlights the remarkable achievements of these scaffolds in the field of bone defect repair in recent years. Lastly, based on the many potential challenges encountered in the preparation and application of BGs, a brief outlook on their future directions is presented. This review may help to provide new ideas for researchers to develop ideal BG-based bone substitutes for bone reconstruction and functional recovery.

Osteoarthritis (OA) is a common degenerative joint disease characterized by progressive cartilage degradation, subchondral bone remodeling, and synovial inflammation. Current treatments cannot halt or reverse OA progression, necessitating the development of novel noninvasive therapies. Therapeutic ultrasound (US), particularly low-intensity pulsed US, has demonstrated efficacy in slowing OA progression. Therapeutic US generates significant thermal and nonthermal effects through noninvasive mechanical forces, exerting biological effects and regulating cell behavior. Therapeutic US has been explored for bone and cartilage repair and shows broad potential in tissue repair when combined with biomaterials. This review summarizes the enhanced or synergistic effects of US and biomaterials in OA. This study elucidated the molecular mechanisms underlying the effects of US on synovium, cartilage, subchondral bone, and mesenchymal stem cells. Notably, the combination of US with various biomaterials can modulate cellular behavior in OA through synergistic effects, including tissue regeneration, enhanced mechanical stimulation, drug delivery, and microenvironment regulation. For each cell type, we summarize the biological mechanisms underlying the therapeutic effects of US and biomaterials, demonstrating their potential to mitigate OA progression. Furthermore, this article explores the limitations and future research prospects of combining US and biomaterials as a therapeutic strategy. Overall, the integration of US and biomaterials holds significant promise as a novel treatment for OA, with potential applications in broader musculoskeletal tissue repair and regenerative medicine. Impact Statement Osteoarthritis (OA) is a complex degenerative disorder that remains challenging to manage. This review highlights the innovative therapeutic potential of combining ultrasound (US), particularly low-intensity pulsed US, with biomaterials for OA treatment. By leveraging synergistic effects such as enhanced tissue repair, targeted drug delivery, and microenvironment regulation, this approach offers a noninvasive and effective strategy to mitigate OA progression and paves the way for advancements in musculoskeletal regenerative medicine.

Multiple studies have been conducted recently to fabricate lyophilized platelet-rich fibrin (LyPRF) as a biological agent. These analyses have also encompassed the integration of LyPRF into various biomaterials for the objective of bone tissue engineering (BTE). However, a definitive manufacturing procedure has not yet been established, and precise data regarding the characterization of LyPRF are still lacking. This systematic literature review aimed to compile existing evidence on the physicochemical and biological properties of this biomaterial as a scaffold for BTE. A comprehensive literature search was performed in SCOPUS, ScienceDirect, PubMed, and Web of Science to identify eligible articles published related to the various in vitro analyses conducted on the biomaterial for its characterization. The inclusion criteria allowed us to concentrate on papers published in English between 2019 and 2025. The study excluded review papers, meta-analyses, editorials, conference pieces, theses, methodological articles, and research that conducted clinical trials or exclusively in vivo analyses. This classification also includes literature with no open access. The preliminary database search produced 3,047 publications, of which only 15 were selected following the application of inclusion and exclusion criteria. LyPRF is beneficial to lengthen the shelf life of the product and can be incorporated into other biomaterials to improve compatibility and reduce degradation time. Therefore, based on the compiled analysis of the included studies, it is found that the surface morphology of LyPRF is irregular, porous, densely populated with fibrin network, and exhibits a uniform aggregation of cells. Furthermore, it is shown that LyPRF demonstrates elements that are analogous to craniofacial bone properties, thereby enhancing its utility in BTE. Additionally, the lyophilization process preserves growth factors present in LyPRF, leading to its consistent and gradual release, increasing the cell proliferation potential of this biomaterial. Existing evidence indicates that LyPRF is a promising candidate for BTE. Future research should prioritize comparative evaluations of fabrication protocols and rigorous biocompatibility testing to establish its suitability as a biomaterial for bioscaffold production in BTE.

Osteoporosis, affecting the entire skeletal system, can cause bone mass to diminish, thereby reducing bone strength and elevating fracture risk. Fracture nonunion and bone defects are common in patients with fractures, and pain and loss of function may cause serious distress. The search for a new therapeutic strategy is essential because of the limited therapeutic options available. Bone marrow mesenchymal stem cells (BMSCs) are crucial for bone metabolism and development due to their high self-renewal capabilities. Wnt signaling is a key pathway that plays a significant role in bone formation by regulating the differentiation of BMSCs. Therefore, the osteogenic differentiation of BMSCs can be regulated by activating Wnt signaling as an idea for bone tissue repair. In this review, we systematically compile and analyze the roles of various drugs, biomolecules, exosomes, and biomaterials in influencing the Wnt/β-catenin signaling pathway during the osteogenic differentiation of BMSCs. It is also discussed how these factors impact on BMSCs and the Wnt/β-catenin pathway. Finally, we also present recent advances in combining bone regeneration materials through these factors, which will help subsequent clinical treatment and translation.

Myofascial pain syndromes, stemming from trigger points within the muscles, represent a prevalent cause of localized or generalized pain in clinical practice. They have a high incidence rate and currently lack specific curative methods. Trigger point injection therapy is the most popular clinical approach, focusing primarily on these trigger points. Injectable drugs like glucose, normal saline, local anesthetics, botulinum toxin type A, steroid preparations, and platelet-rich plasma are available for this purpose. This treatment is advantageous due to its low cost and minimally invasive nature, showing promising results in early clinical use. However, the lack of consensus on the optimal injectable substance presents a significant challenge in clinical practice. This article reviews the progress in clinical research on trigger point injection therapy and drug efficacy, along with precautions for drug administration in managing myofascial pain syndrome. It aims to offer fresh perspectives for future studies and establish a theoretical foundation for treating and caring for myofascial pain syndrome.

Tissue and organ dysfunction are major causes of worldwide morbidity and mortality with all medical specialties being impacted. Tissue engineering is an interdisciplinary field relying on the combination of scaffolds, cells, and biologically active molecules to restore form and function. However, clinical translation is still largely hampered by limitations in vascularization. Consequently, a thorough understanding of the microvasculature is warranted. This review provides an overview of (1) angiogenesis, including sprouting angiogenesis, intussusceptive angiogenesis, vascular remodeling, vascular co-option, and inosculation; (2) strategies for vascularized engineered tissue fabrication such as scaffold modulation, prevascularization, growth factor utilization, and cell-based approaches; (3) guided microvascular development via scaffold modulation with electromechanical cues, 3D bioprinting, and electrospinning; (4) surgical approaches to bridge the micro- and macrovasculatures in order to hasten perfusion; and (5) building specific vasculature in the context of tissue repair and organ transplantation, including skin, adipose, bone, liver, kidney, and lung. Our goal is to provide the reader with a translational overview that spans developmental biology, tissue engineering, and clinical surgery.

Epicatechin (EC)-based derivatives have garnered significant attention for their powerful antioxidant, anti-inflammatory, anticancer, and antibacterial properties, all of which are attributed to the phenolic hydroxyl groups in their structure. These compounds are promising in regenerative medicine, particularly as bioactive components in scaffolds. This review provides an in-depth analysis of the mechanisms by which EC-based materials enhance tissue repair, examining their application in various scaffold forms, such as hydrogels, nanoparticles, and nanofibers. This study also addresses the challenges of stability and bioavailability associated with ECs and proposes encapsulation techniques to overcome these barriers. The potential clinical benefits of ECs in regenerative medicine and their role in fostering advancements in tissue engineering are discussed, making this review a valuable resource for guiding future studies on the integration of ECs into clinical practice.