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Utilization of Hydrogels in Mesenchymal Stem Cell-Based Therapy for Kidney Diseases. 水凝胶在基于间充质干细胞的肾脏疾病治疗中的应用。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-06-01 Epub Date: 2023-11-20 DOI: 10.1089/ten.TEB.2023.0196
Jing Peng, Tinghang Yang, Shanshan Chen, Ningyue Deng, Xinyao Luo, Ruoxi Liao, Baihai Su

Kidney diseases are major global health problems, with high prevalence and mortality. However, current treatment strategies for kidney diseases fail to achieve satisfactory efficacy. Mesenchymal stem cell (MSC)-based therapy has been a promising strategy for treating kidney diseases. Preclinical studies have proven their safety and effectiveness in treating acute kidney injury (AKI) and chronic kidney disease (CKD), but the outcomes of clinical trials have shown very limited clinical efficacy. A variety of innovative approaches have been proposed to enhance the therapeutic potential of MSCs, and hydrogels are attractive candidates. Hydrogels are three-dimensional (3D) networks formed by hydrophilic polymers of natural or synthetic origin with diverse physical and chemical properties. They have been widely applied in the field of drug delivery and regenerative medicine, including MSC-based therapy. Many studies have proven that hydrogels can improve the therapeutic efficacy of MSCs for kidney diseases, but there are still challenges limiting the widespread application of this method. In this review, we introduce the application of MSCs in kidney diseases and the factors that influence therapeutic efficiency and focus on the beneficial effects of hydrogels in MSC-based therapy for AKI and CKD.

肾脏疾病是全球主要的健康问题,发病率和死亡率都很高。然而,目前肾脏疾病的治疗策略未能达到令人满意的疗效。以间充质干细胞(MSC)为基础的治疗已成为治疗肾脏疾病的一种很有前途的策略。临床前研究已经证明其在治疗急性肾损伤和慢性肾脏疾病方面的安全性和有效性,但临床试验的结果显示其临床疗效非常有限。已经提出了各种创新的方法来增强MSCs的治疗潜力,水凝胶是有吸引力的候选者。水凝胶是由具有不同物理和化学性质的天然或合成亲水聚合物形成的三维网络。它们已被广泛应用于药物递送和再生医学领域,包括基于MSC的治疗。许多研究已经证明水凝胶可以提高MSCs对肾脏疾病的治疗效果,但限制这种方法的广泛应用仍然存在挑战。在这篇综述中,我们介绍了MSC在肾脏疾病中的应用以及影响治疗效率的因素,并重点介绍了水凝胶在基于MSC的AKI和CKD治疗中的有益作用。
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引用次数: 0
Three-Dimensional Bioprinting in Vascular Tissue Engineering and Tissue Vascularization of Cardiovascular Diseases. 血管组织工程中的3D生物打印和心血管疾病的组织血管化。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-06-01 Epub Date: 2024-01-05 DOI: 10.1089/ten.TEB.2023.0175
Ben Omondi Ochieng, Leqian Zhao, Zhiyi Ye

In the 21st century, significant progress has been made in repairing damaged materials through material engineering. However, the creation of large-scale artificial materials still faces a major challenge in achieving proper vascularization. To address this issue, researchers have turned to biomaterials and three-dimensional (3D) bioprinting techniques, which allow for the combination of multiple biomaterials with improved mechanical and biological properties that mimic natural materials. Hydrogels, known for their ability to support living cells and biological components, have played a crucial role in this research. Among the recent developments, 3D bioprinting has emerged as a promising tool for constructing hybrid scaffolds. However, there are several challenges in the field of bioprinting, including the need for nanoscale biomimicry, the formulation of hydrogel blends, and the ongoing complexity of vascularizing biomaterials, which requires further research. On a positive note, 3D bioprinting offers a solution to the vascularization problem due to its precise spatial control, scalability, and reproducibility compared with traditional fabrication methods. This paper aims at examining the recent advancements in 3D bioprinting technology for creating blood vessels, vasculature, and vascularized materials. It provides a comprehensive overview of the progress made and discusses the limitations and challenges faced in current 3D bioprinting of vascularized tissues. In addition, the paper highlights the future research directions focusing on the development of 3D bioprinting techniques and bioinks for creating functional materials.

在21世纪,通过材料工程修复受损材料取得了重大进展。然而,在实现适当的血管化方面,大规模人工材料的制造仍然面临着重大挑战。为了解决这个问题,研究人员转向了生物材料和三维(3D)生物打印技术,这种技术可以将多种具有改进的机械和生物性能的生物材料结合起来,模仿天然材料。水凝胶以其支持活细胞和生物成分的能力而闻名,在这项研究中发挥了至关重要的作用。在最近的发展中,3D生物打印已成为构建混合支架的一种很有前途的工具。然而,生物打印领域存在一些挑战,包括对纳米级仿生的需求、水凝胶混合物的配方,以及血管化生物材料的持续复杂性,需要进一步研究。积极的一面是,与传统制造方法相比,3D生物打印具有精确的空间控制、可扩展性和再现性,为血管形成问题提供了解决方案。本文旨在研究3D生物打印技术在创建血管、脉管系统和血管材料方面的最新进展。它全面概述了所取得的进展,并讨论了当前血管化组织的3D生物打印所面临的局限性和挑战。此外,该论文还强调了未来的研究方向,重点是开发3D生物打印技术和用于制造功能材料的生物墨水。
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引用次数: 0
Technological Interventions Enhancing Curcumin Bioavailability in Wound-Healing Therapeutics. 技术干预提高姜黄素在伤口愈合治疗中的生物利用度。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-12-28 DOI: 10.1089/ten.TEB.2023.0085
Hemant Singh, Mukesh Dhanka, Indu Yadav, Sneh Gautam, Showkeen Muzamil Bashir, Narayan Chandra Mishra, Taruna Arora, Shabir Hassan

Wound healing has been a challenge in the medical field. Tremendous research has been carried out to expedite wound healing by fabricating various formulations, some of which are now commercially available. However, owing to their natural source, people have been attracted to advanced formulations with herbal components. Among various herbs, curcumin has been the center of attraction from ancient times for its healing properties due to its multiple therapeutic effects, including antioxidant, antimicrobial, anti-inflammatory, anticarcinogenic, neuroprotective, and radioprotective properties. However, curcumin has a low water solubility and rapidly degrades into inactive metabolites, which limits its therapeutic efficacy. Henceforth, a carrier system is needed to carry curcumin, guard it against degradation, and keep its bioavailability and effectiveness. Different formulations with curcumin have been synthesized, and exist in the form of various synthetic and natural materials, including nanoparticles, hydrogels, scaffolds, films, fibers, and nanoemulgels, improving its bioavailability dramatically. This review discusses the advances in different types of curcumin-based formulations used in wound healing in recent times, concentrating on its mechanisms of action and discussing the updates on its application at several stages of the wound healing process. Impact statement Curcumin is a herbal compound extracted from turmeric root and has been used since time immemorial for its health benefits including wound healing. In clinical formulations, curcumin shows low bioavailability, which mainly stems from the way it is delivered in the body. Henceforth, a carrier system is needed to carry curcumin, guard it against degradation, while maintaining its bioavailability and therapeutic efficacy. This review offers an overview of the advanced technological interventions through tissue engineering approaches to efficiently utilize curcumin in different types of wound healing applications.

伤口愈合一直是医学领域的一个挑战。已经进行了大量的研究,通过制造各种配方来加快伤口愈合,其中一些配方现在已经商业化。然而,由于其天然来源,人们被含有草药成分的先进配方所吸引。在各种草药中,姜黄素由于其多种治疗作用,包括抗氧化、抗菌、抗炎、抗癌、神经保护和放射保护作用,自古以来就因其治疗特性而备受关注。然而,姜黄素的水溶性低,并迅速降解为无活性代谢产物,这限制了其治疗效果。因此,需要一种载体系统来携带姜黄素,防止其降解,并保持其生物利用度和有效性。姜黄素的不同配方已经被合成,并以各种合成和天然材料的形式存在,包括纳米颗粒、水凝胶、支架、薄膜、纤维和纳米乳液,极大地提高了其生物利用度。这篇综述讨论了近年来用于伤口愈合的不同类型姜黄素制剂的进展,重点讨论了其作用机制,并讨论了其在伤口愈合过程的几个阶段应用的最新进展。
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引用次数: 0
Three-Dimensional Scaffolds for Intestinal Cell Culture: Fabrication, Utilization, and Prospects. 用于肠道细胞培养的三维支架:制造、利用和前景。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-09-22 DOI: 10.1089/ten.TEB.2023.0124
Tiange Liu, Jia Gu, Caili Fu, Lingshan Su
<p><p>The intestine is a visceral organ that integrates absorption, metabolism, and immunity, which is vulnerable to external stimulus. Researchers in the fields such as food science, immunology, and pharmacology have committed to developing appropriate <i>in vitro</i> intestinal cell models to study the intestinal absorption and metabolism mechanisms of various nutrients and drugs, or pathogenesis of intestinal diseases. In the past three decades, the intestinal cell models have undergone a significant transformation from conventional two-dimensional cultures to three-dimensional (3D) systems, and the achievements of 3D cell culture have been greatly contributed by the fabrication of different scaffolds. In this review, we first introduce the developing trend of existing intestinal models. Then, four types of scaffolds, including Transwell, hydrogel, tubular scaffolds, and intestine-on-a-chip, are discussed for their 3D structure, composition, advantages, and limitations in the establishment of intestinal cell models. Excitingly, some of the <i>in vitro</i> intestinal cell models based on these scaffolds could successfully mimic the 3D structure, microenvironment, mechanical peristalsis, fluid system, signaling gradients, or other important aspects of the original human intestine. Furthermore, we discuss the potential applications of the intestinal cell models in drug screening, disease modeling, and even regenerative repair of intestinal tissues. This review presents an overview of state-of-the-art scaffold-based cell models within the context of intestines, and highlights their major advances and applications contributing to a better knowledge of intestinal diseases. Impact statement The intestine tract is crucial in the absorption and metabolism of nutrients and drugs, as well as immune responses against external pathogens or antigens in a complex microenvironment. The appropriate experimental cell model <i>in vitro</i> is needed for in-depth studies of intestines, due to the limitation of animal models in dynamic control and real-time assessment of key intestinal physiological and pathological processes, as well as the "R" principles in laboratory animal experiments. Three-dimensional (3D) scaffold-based cell cultivation has become a developing tendency because of the superior cell proliferation and differentiation and more physiologically relevant environment supported by the customized 3D scaffolds. In this review, we summarize four types of up-to-date 3D cell culture scaffolds fabricated by various materials and techniques for a better recapitulation of some essential physiological and functional characteristics of original intestines compared to conventional cell models. These emerging 3D intestinal models have shown promising results in not only evaluating the pharmacokinetic characteristics, security, and effectiveness of drugs, but also studying the pathological mechanisms of intestinal diseases at cellular and molecular levels. Impor
肠道是一个集吸收、代谢和免疫于一体的内脏器官,易受外部刺激。食品科学、免疫学和药理学等领域的研究人员致力于开发合适的体外肠道细胞模型,以研究各种营养物质和药物的肠道吸收和代谢机制,或肠道疾病的发病机制。在过去的三十年里,肠道细胞模型经历了从传统的二维培养到三维(3D)系统的重大转变,不同支架的制造对3D细胞培养的成就做出了巨大贡献。在这篇综述中,我们首先介绍了现有肠道模型的发展趋势。然后,讨论了四种类型的支架,包括Transwell、水凝胶、管状支架和芯片肠道支架,以了解它们的3D结构、组成、优点和在建立肠道细胞模型方面的局限性。令人兴奋的是,一些基于这些支架的体外肠道细胞模型可以成功模拟原始人类肠道的3D结构、微环境、机械蠕动、流体系统、信号梯度或其他重要方面。此外,我们还讨论了肠道细胞模型在药物筛选、疾病建模甚至肠道组织再生修复中的潜在应用。这篇综述概述了肠道中最先进的基于支架的细胞模型,并强调了它们的主要进展和应用,有助于更好地了解肠道疾病。
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引用次数: 0
Trends in Adipose-Derived Stem Cell-Conditioned Medium: A Bibliometric and Visualized Review. 脂肪干细胞调节培养基的发展趋势:文献计量和可视化回顾。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-09-04 DOI: 10.1089/ten.TEB.2023.0107
Yu Cheng, Guangpeng Liu

Adipose-derived stem cell-conditioned medium (ADSC-CM) has been widely studied and used as a stem cell-based cell-free therapy. Due to the explosion of scientific publications in this field, it is difficult to review all relevant publications systematically, not mention quantitively. In this study, we combined bibliometrics with the conventional review method to summarize, analyze, and visualize the characteristics of nearly all published articles related to ADSC-CM using CiteSpace-a bibliometrics software. We applied this software to quantitively and vividly show (a) annual publications and citations; (b) distributions and co-occurrence networks of countries/regions, authors, journals, and institutions; (c) keyword co-occurrence networks and clusters in different time periods; (d) cocitation networks of references; and (e) ongoing challenges and new topics in ADSC-CM. Altogether, we found that ADSC-CM is at a hot stage with an increasing number of publications and citations, extensive and close scientific collaborations, and dense cocited networks. Impact statement To our best knowledge, it is the first bibliometric and visualized review in the field of adipose-derived stem cell-conditioned medium (ADSC-CM). This review systematically and quantitatively revealed the developments, challenges, and emerging hot spots of ADSC-CM, providing a panoramic view to assist researchers to decide the direction of their future study in the fields of ADSCs and CM derived from stem cells.

脂肪来源干细胞调节培养基(ADSC-CM)作为一种基于干细胞的无细胞疗法已被广泛研究和使用。由于该领域的科学论文数量激增,很难对所有相关论文进行系统性综述,更不用说定量综述了。在本研究中,我们将文献计量学与传统的综述方法相结合,利用文献计量学软件 CiteSpace 对几乎所有已发表的 ADSC-CM 相关文章的特点进行了总结、分析和可视化。我们应用该软件定量而生动地展示了:(a)年度论文发表和引用情况;(b)国家/地区、作者、期刊和机构的分布和共现网络;(c)不同时间段的关键词共现网络和集群;(d)参考文献的共现网络;以及(e)ADSC-CM领域正在面临的挑战和新课题。总之,我们发现 ADSC-CM 正处于一个热点阶段,其论文数量和引用次数不断增加,科学合作广泛而密切,共现网络密集。影响声明 据我们所知,这是脂肪源性干细胞调节培养基(ADSC-CM)领域的第一篇文献计量和可视化综述。这篇综述系统地、定量地揭示了 ADSC-CM 的发展、挑战和新兴热点,为研究人员提供了一个全景视角,帮助他们决定未来在 ADSCs 和干细胞培养基领域的研究方向。
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引用次数: 0
Extracellular Vesicles from Urine-Derived Stem Cell for Tissue Engineering and Regenerative Medicine. 用于组织工程和再生医学的尿来源干细胞的细胞外小泡。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-09-22 DOI: 10.1089/ten.TEB.2023.0100
Yi-Jun Dong, Juan-Juan Hu, Yu-Ting Song, Ya-Ya Gao, Mei-Jun Zheng, Chen-Yu Zou, Ming Xiong, Jesse Li-Ling, Hui Yang, Hui-Qi Xie

The potential of urine-derived stem cells (USCs) for tissue engineering and regenerative medicine has attracted much attention during the last few decades. However, it has been suggested that the effects of the USCs may be endowed by their paracrine extracellular vesicles (EVs) rather than their differentiation. Compared with the USCs, the USC-EVs can cross the barriers more easily and safely, and their inclusions may mediate intercellular communication and promote the tissue repair. This article has summarized the current knowledge and applications about the USC-EVs in tissue engineering and regenerative medicine, and discussed the prospects and challenges for using them as an alternative to cell therapy. Impact statement Urine-derived stem cells (USCs) represent a newly discovered type of stem cells, and studies have proved that the beneficial effects of the USCs may be manifested through their paracrine extracellular vesicles (EVs) rather than through their own differentiation, which opens up new avenues for tissue engineering and regenerative medicine strategies. Therefore, this review aims to summarize the latest research progress and potential clinical applications of the USC-EVs, highlighting the promising potential of the USC-EVs as a therapeutic option in kidney regeneration, genital regeneration, nerve regeneration, bone and cartilage regeneration, and wound healing.

在过去的几十年里,尿液来源的干细胞在组织工程和再生医学中的潜力引起了人们的广泛关注。然而,有人认为,USCs的作用可能是由其旁分泌细胞外小泡(EV)赋予的,而不是由其分化赋予的。与USCs相比,USC-EVs可以更容易、更安全地跨越屏障,其内含物可以介导细胞间通讯,促进组织修复。本文总结了USC EV在组织工程和再生医学中的最新知识和应用,并讨论了将其作为细胞治疗的替代品的前景和挑战。
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引用次数: 0
Nanoparticle and Nanotopography-Induced Activation of the Wnt Pathway in Bone Regeneration. 纳米颗粒和纳米形貌诱导骨再生中Wnt途径的激活。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-11-20 DOI: 10.1089/ten.TEB.2023.0108
Chitra Jagannathan, Rachel Waddington, Wayne Nishio Ayre
<p><p><b><i>Background and Aims:</i></b> Recent research has focused on developing nanoparticle and nanotopography-based technologies for bone regeneration. The Wingless-related integration site (Wnt) signaling pathway has been shown to play a vital role in this process, in particular in osteogenic differentiation and proliferation. The exact mechanisms by which nanoparticles and nanotopographies activate the Wnt signaling pathway, however, are not fully understood. This review aimed to elucidate the mechanisms by which nanoscale technologies activate the Wnt signaling pathway during bone regeneration. <b><i>Methods:</i></b> The terms "Wnt," "bone," and "nano*" were searched on PubMed and Ovid with no date limit. Only original research articles related to Wnt signaling and bone regeneration in the context of nanotopographies, nanoparticles, or scaffolds with nanotopographies/nanoparticles were reviewed. <b><i>Results:</i></b> The primary mechanism by which nanoparticles activated the Wnt pathway was by internalization through the endocytic pathway or diffusion through the cell membrane, leading to accumulation of nonphosphorylated β-catenin in the cytoplasm and subsequently downstream osteogenic signaling (e.g., upregulation of runt-related transcription factor 2 [RUNX2]). The specific size of the nanoparticles and the process of endocytosis itself has been shown to modulate the Wnt-β-catenin pathway. Nanotopographies were shown to directly activate frizzled receptors, initiating Wnt/β-catenin signaling. Additional studies showed nanotopographies to activate the Wnt/calcium (Wnt/Ca<sup>2+</sup>)-dependent and Wnt/planar cell polarity pathways through nuclear factor of activated T cells, and α5β1 integrin stimulation. Finally, scaffolds containing nanotopographies/nanoparticles were found to induce Wnt signaling through a combination of ion release (e.g., lithium, boron, lanthanum, and icariin), which inhibited glycogen synthase kinase 3 beta (GSK-3β) activity, and through similar mechanisms to the nanotopographies. <b><i>Conclusion:</i></b> This review concludes that nanoparticles and nanotopographies cause Wnt activation through several different mechanisms, specific to the size, shape, and structure of the nanoparticles or nanotopographies. Endocytosis-related mechanisms, integrin signaling and ion release were the major mechanisms identified across nanoparticles, nanotopographies, and scaffolds, respectively. Knowledge of these mechanisms will help develop more effective targeted nanoscale technologies for bone regeneration. Impact statement Nanoparticles and nanotopographies can activate the Wingless-related integration site (Wnt) signaling pathway, which is essential for bone regeneration. This review has identified that activation is due to endocytosis, integrin signaling and ion release, depending on the size, shape, and structure of the nanoparticles or nanotopographies. By identifying and further understanding these mechanisms, more effe
背景和目的:最近的研究重点是开发基于纳米颗粒和纳米形貌的骨再生技术。Wnt信号通路已被证明在这一过程中起着至关重要的作用,特别是在成骨分化和增殖中。然而,纳米颗粒和纳米拓扑结构激活Wnt信号通路的确切机制尚不完全清楚。这篇综述旨在阐明纳米技术在骨再生过程中激活Wnt信号通路的机制。方法:在PubMed和Ovid上搜索术语“Wnt”、“bone”和“nano*”,没有日期限制。仅回顾了在纳米拓扑结构、纳米颗粒或具有纳米拓扑结构或纳米颗粒的支架的背景下与Wnt信号传导和骨再生相关的原始研究文章。结果:纳米颗粒激活Wnt途径的主要机制是通过内吞途径内化或通过细胞膜扩散,导致非磷酸化的β-连环蛋白在细胞质中积累,随后产生下游成骨信号(例如RUNX2的上调)。纳米颗粒的特定大小和内吞过程本身已被证明可以调节Wnt-β-连环蛋白途径。纳米形貌显示可以直接激活卷曲的受体,启动Wnt/β-catenin通路信号传导。其他研究表明,纳米拓扑图通过活化T细胞的核因子和α5β1整合素刺激来激活Wnt/Ca2+依赖性和Wnt/平面细胞极性途径。最后,发现含有纳米拓扑图/纳米颗粒的支架通过抑制GSK-3β活性的离子释放(如锂、硼、镧和icariin)的组合,以及通过与纳米拓扑图类似的机制,诱导Wnt信号传导。结论:这篇综述的结论是,纳米颗粒和纳米拓扑结构通过几种不同的机制引起Wnt活化,这些机制与纳米颗粒或纳米拓扑结构的大小、形状和结构有关。细胞内积相关机制、整合素信号传导和离子释放分别是纳米颗粒、纳米拓扑结构和支架的主要机制。了解这些机制将有助于开发更有效的骨再生靶向纳米技术。
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引用次数: 0
Global Bibliometric and Visualized Analysis of Tracheal Tissue Engineering Research. 气管组织工程研究的全球文献计量和可视化分析。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-09-28 DOI: 10.1089/ten.TEB.2023.0129
Jianwei Zhu, Yi Lu, Yibo Shan, Lei Yuan, Qiang Wu, Zhiming Shen, Fei Sun, Hongcan Shi

The development of tracheal tissue engineering (TTE) has seen a rapid growth in recent years. The purpose of this study was to investigate the global status, trends, and hotspots of TTE research based on bibliometrics and visualization analysis. Publications related to TTE were retrieved and included in the Web of Science Core Collection. VOSviewer and CiteSpace were used to generate knowledge maps. Six hundred fifty-five publications were identified, and the quantity of the annual publications worldwide was on the increase. International collaboration is a widespread reality. The United States led the world in the field of trachea tissue engineering, whereas University College London was the institution with the greatest contribution. In addition, Biomaterials had a great influence in this field, attracting the largest number of papers. Moreover, the topics of TTE research largely concentrated on the biomechanical scaffold preparation, the vascularization and epithelialization of scaffold, the tracheal cartilage regeneration, and the tissue-engineered tracheal transplantation. And the research on the application of decellularization and 3D printing for the construction of a tissue-engineered trachea was likely to receive more widespread attention in the future. Impact statement In recent years, tracheal tissue engineering (TTE) has experienced rapid growth. In this study, we investigated the worldwide status and trends of TTE research, and revealed the countries, institutions, journals, and authors that had made significant contributions to the field of TTE. Moreover, the possible research hotspots in the future were predicted. According to our research, researchers can gain a better understanding of the trends in this field, and stay informed of the most current research by tracking key journals, institutions, and authors.

近年来,气管组织工程(TTE)发展迅速。本研究的目的是基于文献计量学和可视化分析,调查TTE研究的全球现状、趋势和热点。检索到了与TTE相关的出版物,并将其纳入了网络科学核心收藏。VOSviewer和CiteSpace用于生成知识地图。已确定了605种出版物,全球年度出版物的数量正在增加。国际合作是一个普遍存在的现实。美国在气管组织工程领域处于世界领先地位,而伦敦大学学院是贡献最大的机构。此外,生物材料在这一领域有着巨大的影响力,吸引了最多的论文。此外,TTE研究的主题主要集中在生物力学支架的制备、支架的血管化和上皮化、气管软骨再生和组织工程化气管移植。脱细胞和3D打印在组织工程气管构建中的应用研究在未来可能会受到更广泛的关注。
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引用次数: 0
Biomechanical Aspects in Bone Tumor Engineering. 骨肿瘤工程中的生物力学方面。
IF 6.4 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-11-16 DOI: 10.1089/ten.TEB.2023.0106
Ksenia Menshikh, Ivana Banicevic, Bojana Obradovic, Lia Rimondini

In the past decades, anticancer drug development brought the field of tumor engineering to a new level by the need of robust test systems. Simulating tumor microenvironment in vitro remains a challenge, and osteosarcoma-the most common primary bone cancer-is no exception. The growing evidence points to the inevitable connection between biomechanical stimuli and tumor chemosensitivity and aggressiveness, thus making this component of the microenvironment a mandatory requirement to the developed models. In this review, we addressed the question: is the "in vivo - in vitro" gap in osteosarcoma engineering bridged from the perspective of biomechanical stimuli? The most notable biomechanical cues in the tumor cell microenvironment are observed and compared in the contexts of in vivo conditions and engineered three-dimensional in vitro models. Impact statement The importance of biomechanical stimuli in three-dimensional in vitro models for drug testing is becoming more pronounced nowadays. This review might assist in understanding the key players of the biophysical environment of primary bone cancer and the current state of bone tumor engineering from this perspective.

在过去的几十年里,由于需要强大的测试系统,抗癌药物的开发将肿瘤工程领域提升到了一个新的水平。体外模拟肿瘤微环境仍然是一个挑战,骨肉瘤——最常见的原发性癌症——也不例外。越来越多的证据表明,生物力学刺激与肿瘤化学敏感性和侵袭性之间存在不可避免的联系,因此,微环境的这一组成部分成为开发模型的强制性要求。在这篇综述中,我们提出了一个问题:从生物力学刺激的角度来看,骨肉瘤工程中的“体内-体外”差距是否弥合了?在体内条件和工程三维体外模型的背景下,观察和比较了肿瘤细胞微环境中最显著的生物力学线索。
{"title":"Biomechanical Aspects in Bone Tumor Engineering.","authors":"Ksenia Menshikh, Ivana Banicevic, Bojana Obradovic, Lia Rimondini","doi":"10.1089/ten.TEB.2023.0106","DOIUrl":"10.1089/ten.TEB.2023.0106","url":null,"abstract":"<p><p>In the past decades, anticancer drug development brought the field of tumor engineering to a new level by the need of robust test systems. Simulating tumor microenvironment <i>in vitro</i> remains a challenge, and osteosarcoma-the most common primary bone cancer-is no exception. The growing evidence points to the inevitable connection between biomechanical stimuli and tumor chemosensitivity and aggressiveness, thus making this component of the microenvironment a mandatory requirement to the developed models. In this review, we addressed the question: is the \"<i>in vivo - in vitro</i>\" gap in osteosarcoma engineering bridged from the perspective of biomechanical stimuli? The most notable biomechanical cues in the tumor cell microenvironment are observed and compared in the contexts of <i>in vivo</i> conditions and engineered three-dimensional <i>in vitro</i> models. Impact statement The importance of biomechanical stimuli in three-dimensional <i>in vitro</i> models for drug testing is becoming more pronounced nowadays. This review might assist in understanding the key players of the biophysical environment of primary bone cancer and the current state of bone tumor engineering from this perspective.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"217-229"},"PeriodicalIF":6.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11001506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41213835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematic Review of Cell Therapy Efficacy in Human Chronic Spinal Cord Injury. 细胞治疗人类慢性脊髓损伤疗效的系统评价。
IF 5.1 2区 医学 Q2 CELL & TISSUE ENGINEERING Pub Date : 2024-04-01 Epub Date: 2023-12-26 DOI: 10.1089/ten.TEB.2023.0130
Reyhaneh Abolghasemi, Esmat Davoudi-Monfared, Fakhri Allahyari, Gholamreza Farzanegan

Spinal cord injury (SCI) is one of the most debilitating problems for humans. About 6 months after the initial injury, a cascade of secondary cellular and molecular events occurs and the primary damage enters the chronic phase. Current treatments are not curative. One of the new treatment methods is the use of cell therapy, which is gradually being tested in clinical trials to improve the symptoms of SCI patients. In this review article, we investigated the effect of different cell therapy trials in improving patients' symptoms and their paraclinical indicators. In the 72 final reviewed studies with 1144 cases and 186 controls, 20 scores were recorded as outcomes. We categorized the scores into seven groups. In upper extremity motor score, daily living function, trunk stability, postural hypotension, somatosensory evoked potential, and motor evoked potential scores, the bone marrow hematopoietic stem cell therapy had a more healing effect. In the International Association of Neurorestoratology SCI Functional Rating Scale, light touch score, bowel function, decreased spasticity, Visual Analog Scale, and electromyography scores, the bone marrow mesenchymal stem cell had more impact. The olfactory ensheathing cell had a greater effect on lower extremity motor score and pinprick scores than other cells. The embryonic stem cell had the greatest effect in improving the important score of the American Spinal Injury Association scale. Based on the obtained results, it seems that a special cell should be used to improve each symptom of patients with chronic SCI, and if the improvement of several harms is involved, the combination of cells may be effective. Impact statement Compared to similar review articles published so far, we reviewed the largest number of published articles, and so the largest number of cases and controls, and the variety of cells we examined was more than other published articles. We concluded that different cells are effective for improving the symptoms and paraclinical indicators of patients with chronic spinal cord injury. Bone marrow hematopoietic stem cell and bone marrow mesenchymal stem cell have had the higher overall mean effect in more scores (each in six scores). If the improvement of several harms is involved, the combination of cells may be effective.

脊髓损伤是人类最虚弱的问题之一。六个月后,病变相对稳定,疾病进入慢性期。目前的治疗方法无法治愈。利用独特的细胞治疗方法在慢性脊柱损伤中取得进展,为患者的询问和治疗打开了现代信任。在这篇综述文章中,我们研究了不同细胞治疗试验在改善患者症状方面的效果。在对1144例病例和186名对照进行的72项最终审查研究中,20分被记录为结果。在上肢运动评分、日常生活功能、躯干稳定性、体位性低血压、体感诱发电位和运动诱发电位评分方面,骨髓造血干细胞治疗的疗效较好。在国际神经修复学协会脊髓损伤功能评定量表、轻度触摸评分、排便功能、痉挛减轻、视觉模拟量表和肌电图评分中,骨髓间充质干细胞的影响更大。嗅鞘细胞对下肢运动评分和针刺评分的影响大于其他细胞。胚胎干细胞在提高美国脊髓损伤协会量表的重要评分方面效果最大。根据所获得的结果,似乎应该使用一种特殊的细胞来改善慢性脊髓损伤患者的每一种症状,如果涉及几种危害的改善,细胞的组合可能是有效的。
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Tissue Engineering. Part B, Reviews
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