Pub Date : 2022-04-26DOI: 10.23902/trkjnat.1059974
Zeynep Sule Topatan, H. Katı
Secondary metabolites obtained from bacteria have an important role their diverse applications and use in various industrial fields. Today, the constant search for novel enzyme and antibiotic are carried out from actinomycetes obtained from different sources. Actinomycetes obtained from the extreme environments are known to be producers of novel enzymes and antibiotic with great industrial potential. In this study, Actinomycetes isolation was performed by serial dilution method from compost obtained Cystoseira sp. algae collected from Black Sea coast. Enzyme contents by qualitative method and antibacterial activities by using cross streak method of isolated actinomycetes were investigated. Identification of 6 actinomycetes isolates with high enzyme and antibacterial activity was performed according to morphological, physiological and 16S rDNA sequence analysis. A total of 73 actinomycetes isolates (BSC) were obtained from the compost samples. When the enzyme activities of actinomycetes isolates were evaluated, it is found that 68.5% of these have amylase, 100% have cellulase, 47.9% have chitinase, 94.5% have pectinase, 98.6% have protease and 96.3% have lipase/esterase enzyme production. The highest antibacterial activity was observed against Staphylococcus aureus and Enterococcus faecalis, which inhibited 98.6 and 84.9 % of the tested actinomycetes isolates, respectively. It has been determined that the actinomycetes isolates (BSC-13, BSC-17, BSC-37, BSC-38, BSC-45 and BSC-49) with high enzyme and antibacterial activity belong to Streptomyces genus according to 16S rDNA sequence analysis. Secondary metabolites from actinomycetes are likely to be used in different industrial areas in the future.
{"title":"SCREENING OF ACTINOMYCETES FROM Cystoseira barbata (Stackhouse) C. Agardh COMPOST FOR THEIR ENZYME AND ANTIBACTERIAL ACTIVITIES","authors":"Zeynep Sule Topatan, H. Katı","doi":"10.23902/trkjnat.1059974","DOIUrl":"https://doi.org/10.23902/trkjnat.1059974","url":null,"abstract":"Secondary metabolites obtained from bacteria have an important role their diverse applications and use in various industrial fields. Today, the constant search for novel enzyme and antibiotic are carried out from actinomycetes obtained from different sources. Actinomycetes obtained from the extreme environments are known to be producers of novel enzymes and antibiotic with great industrial potential. \u0000In this study, Actinomycetes isolation was performed by serial dilution method from compost obtained Cystoseira sp. algae collected from Black Sea coast. Enzyme contents by qualitative method and antibacterial activities by using cross streak method of isolated actinomycetes were investigated. Identification of 6 actinomycetes isolates with high enzyme and antibacterial activity was performed according to morphological, physiological and 16S rDNA sequence analysis. \u0000A total of 73 actinomycetes isolates (BSC) were obtained from the compost samples. When the enzyme activities of actinomycetes isolates were evaluated, it is found that 68.5% of these have amylase, 100% have cellulase, 47.9% have chitinase, 94.5% have pectinase, 98.6% have protease and 96.3% have lipase/esterase enzyme production. The highest antibacterial activity was observed against Staphylococcus aureus and Enterococcus faecalis, which inhibited 98.6 and 84.9 % of the tested actinomycetes isolates, respectively. It has been determined that the actinomycetes isolates (BSC-13, BSC-17, BSC-37, BSC-38, BSC-45 and BSC-49) with high enzyme and antibacterial activity belong to Streptomyces genus according to 16S rDNA sequence analysis. \u0000Secondary metabolites from actinomycetes are likely to be used in different industrial areas in the future.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79151443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-25DOI: 10.23902/trkjnat.1043241
Nuraniye Eruygur, N. Özpınar, Hülya Özpınar, M. Atas, M. Tekin, O. Cevik
The objective of the study to evaluate methanolic extracts of aerial parts of three Hypericum species: Hypericum perforatum, Hypericum thymbrifolium and Hypericum thymopsis for their in vitro antioxidant, anti-Trichomonas vaginalis activity, antimicrobial and cytotoxic activity. The antioxidant activity of methanol extract was tested with DPPH, iron chelating, total phenol and total flavonoid content assays. Cytotoxicity and apoptosis were evaluated on MCF-7 breast cancer and PC3 prostate cancer cell line. Antimicrobial activity were evaluated by a microdilution method on five microorganisms. The obtained results of the present study revealed that methanol extract of H. thymbrifolium, H. perforatum and H. thymopsis have significant antioxidant, cytotoxic activity. The results are provided the basic research data for further phytochemical and biological activity guided investigation on thes species for identicate and isolation of potential drug active compounds.
{"title":"Biological activity evaluation of three Hypericum species wild growing in Sivas province of Turkey","authors":"Nuraniye Eruygur, N. Özpınar, Hülya Özpınar, M. Atas, M. Tekin, O. Cevik","doi":"10.23902/trkjnat.1043241","DOIUrl":"https://doi.org/10.23902/trkjnat.1043241","url":null,"abstract":"The objective of the study to evaluate methanolic extracts of aerial parts of three Hypericum species: Hypericum perforatum, Hypericum thymbrifolium and Hypericum thymopsis for their in vitro antioxidant, anti-Trichomonas vaginalis activity, antimicrobial and cytotoxic activity. The antioxidant activity of methanol extract was tested with DPPH, iron chelating, total phenol and total flavonoid content assays. Cytotoxicity and apoptosis were evaluated on MCF-7 breast cancer and PC3 prostate cancer cell line. Antimicrobial activity were evaluated by a microdilution method on five microorganisms. The obtained results of the present study revealed that methanol extract of H. thymbrifolium, H. perforatum and H. thymopsis have significant antioxidant, cytotoxic activity. The results are provided the basic research data for further phytochemical and biological activity guided investigation on thes species for identicate and isolation of potential drug active compounds.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82931181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-06DOI: 10.23902/trkjnat.1037376
M. Yildiz, N. Tosun
{"title":"MOLECULAR CHARACTERIZATION OF BLACK FOOT DISEASE PATHOGENS IN GRAPEVINE NURSERIES AND EVALUATION OF FUNGICIDES FOR CONTROL OF THE MOST VIRULENT ISOLATES","authors":"M. Yildiz, N. Tosun","doi":"10.23902/trkjnat.1037376","DOIUrl":"https://doi.org/10.23902/trkjnat.1037376","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"20 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81034174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-28DOI: 10.23902/trkjnat.1023345
A. Aykal, E. Seven
: This study was conducted on the Geometridae fauna of Hasankeyf district of Batman between 2018 and 2020. 27 different localities were visited and 2.870 specimens in total were collected using insect net and UV light traps mostly in nighttimes, since the majority of Geometridae species are nocturnal. The taxonomic investigations of the collected specimens revealed presence of 66 species within 39 genera in 5 subfamilies (Desmobathrinae, Geometrinae, Ennominae, Larentiinae, Sterrhinae) in the study area. Gnophos chorista Wehrli, 1939, is reported as a new record for the Turkish fauna and photographs of
{"title":"THE GEOMETRID MOTHS (LEPIDOPTERA) OF THE ANCIENT CITY HASANKEYF (BATMAN) AND A NEW SPECIES FOR THE TURKISH FAUNA","authors":"A. Aykal, E. Seven","doi":"10.23902/trkjnat.1023345","DOIUrl":"https://doi.org/10.23902/trkjnat.1023345","url":null,"abstract":": This study was conducted on the Geometridae fauna of Hasankeyf district of Batman between 2018 and 2020. 27 different localities were visited and 2.870 specimens in total were collected using insect net and UV light traps mostly in nighttimes, since the majority of Geometridae species are nocturnal. The taxonomic investigations of the collected specimens revealed presence of 66 species within 39 genera in 5 subfamilies (Desmobathrinae, Geometrinae, Ennominae, Larentiinae, Sterrhinae) in the study area. Gnophos chorista Wehrli, 1939, is reported as a new record for the Turkish fauna and photographs of","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79861604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-25DOI: 10.23902/trkjnat.1000196
Zeynep Danisman, Süleyman İnan, Esma Özsoy, E. Yörük, T. Yli-Mattila
{"title":"TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum","authors":"Zeynep Danisman, Süleyman İnan, Esma Özsoy, E. Yörük, T. Yli-Mattila","doi":"10.23902/trkjnat.1000196","DOIUrl":"https://doi.org/10.23902/trkjnat.1000196","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"472 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88859113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IN VITRO AND IN SILICO EVALUATION OF THYMOQUINONE AS POTENTIAL ANTICANCER AGENT IN HUMAN ACUTE MYELOID LEUKEMIA HL-60 CELLS","authors":"Neslihan TEKİN KARACAER","doi":"10.23902/trkjnat.999403","DOIUrl":"https://doi.org/10.23902/trkjnat.999403","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"29 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75030046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-28DOI: 10.23902/trkjnat.1008271
Asiye Uzun, O. Demirözer
{"title":"POPULATION FLUCTUATION OF HAPLOTHRIPS TRITICI (KURDJUMOV, 1912","authors":"Asiye Uzun, O. Demirözer","doi":"10.23902/trkjnat.1008271","DOIUrl":"https://doi.org/10.23902/trkjnat.1008271","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90446960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PLASTIC MULCHING OR CONVENTIONAL CULTIVATION OF LAVENDER FLOWER: WHAT INFLUENCE ON THE YIELD, ESSENTIAL OIL AND THEIR NEUROPROTECTIVE EFFECTS?","authors":"N. Sekeroglu, Gul Cimen, M. Kulak, S. Gezici","doi":"10.23902/trkjnat.992275","DOIUrl":"https://doi.org/10.23902/trkjnat.992275","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"74 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77334150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DIFFERENTIATED PRE-ADIPOCYTES ACT AS A DRIVING FORCE FOR PROSTATE CANCER PROLIFERATION, MIGRATION AND EPITHELIAL-MESENCHYMAL TRANSITION","authors":"Hüseyin Abdik, Arya Lal Erkilinçoğlu, F. Şahin","doi":"10.23902/trkjnat.978266","DOIUrl":"https://doi.org/10.23902/trkjnat.978266","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"2013 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87886819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HEMATOLOGY AND PLASMA BIOCHEMISTRY OF WILD-CAUGHT GREATER MOUSE-EARED BAT Myotis myotis (CHIROPTERA: VESPERTILIONIDAE)","authors":"E. Paksuz","doi":"10.23902/trkjnat.962609","DOIUrl":"https://doi.org/10.23902/trkjnat.962609","url":null,"abstract":"","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90685091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：