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THE FIRST REPORT OF GEOSMIN AND 2-METHYLISOBORNEOL PRODUCER CYANOBACTERIA FROM TURKISH FRESHWATERS 土耳其淡水中土臭素和2-甲基异龙脑蓝藻的首次报道
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-06-16 DOI: 10.23902/trkjnat.884423
Zuhal Tunç, R. Akçaalan, Latife Köker, Meriç Albay
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引用次数: 1
BIOMOLECULES AND NATURAL PRODUCTS REGULATE THE HUMAN ACE2 GENE EXPRESSION ON THE LUNG CANCER CELLS 生物分子和天然产物调控人ace2基因在肺癌细胞上的表达
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-06-15 DOI: 10.23902/trkjnat.896013
Kaan Hürkan, Ş. Arslan, M. Atalar, A. Aydin, I. Demirtaş, Dogukan Mutlu, Bahattin Tabar, M. Alma
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引用次数: 0
SYNTHESIS, CHARACTERIZATION AND BIOCOMPATIBILITY OF PLANT-OIL BASED HYDROGELS 植物油基水凝胶的合成、表征及生物相容性研究
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-06-10 DOI: 10.23902/trkjnat.925742
Ozlem Yalcin Capan, Pinar Cakir Hatir
Biocompatible hydrogels are used in a variety of biomedical applications, including tissue scaffolds, drug delivery systems, lab/organ-on-a-chips, biosensors, cell-culture studies and contact lenses. The demand for novel and functional monomers to be used in hydrogel synthesis is increasing as the number of biomedical applications and need for biomaterials increase. The purpose of the study was to develop novel hydrogels from renewable materials. Acrylated methyl ricinoleate, a plant oil-based monomer, was used as the renewable material. The effects of acrylated methyl ricinoleate/N-isopropyl acrylamide molar ratio on hydrogel structural properties, thermal stability and in vitro cytotoxicity were studied. FTIR spectroscopy was used to characterize the structural properties of the hydrogels, while TGA was used to characterize the thermal properties. HEK293 and Cos-7 cell lines were used to test the cytotoxicity of the monomers and hydrogels. IC50 values for acrylated methyl ricinoleate and N-isopropyl acrylamide were found to be greater than 25 mg/mL. Cell viability of hydrogels containing 50% or more acrylated methyl ricinoleate was greater than 60%, while hydrogel biocompatibility decreased with decreasing molar ratio of acrylated methyl ricinoleate. Cells showed a minimum viability of 80% when incubated in hydrogel degradation products. An environmentally friendly synthesis method was developed and novel biocompatible hydrogels from renewable materials were produced for biomedical applications.
生物相容性水凝胶用于各种生物医学应用,包括组织支架、药物输送系统、实验室/芯片上的器官、生物传感器、细胞培养研究和隐形眼镜。随着生物医学应用的增加和对生物材料的需求增加,对用于水凝胶合成的新型功能单体的需求也在增加。本研究的目的是利用可再生材料开发新型水凝胶。以植物油基单体丙烯化蓖麻油酸甲酯为可再生材料。研究了丙烯化蓖麻油酸甲酯/ n -异丙基丙烯酰胺摩尔比对水凝胶结构性能、热稳定性和体外细胞毒性的影响。利用红外光谱(FTIR)表征了水凝胶的结构性质,热重分析仪(TGA)表征了水凝胶的热性能。用HEK293和Cos-7细胞株检测单体和水凝胶的细胞毒性。丙烯酸化蓖麻油酸甲酯和n -异丙基丙烯酰胺的IC50值均大于25 mg/mL。丙烯酸甲基蓖麻油酸含量≥50%的水凝胶细胞活力大于60%,但随着丙烯酸甲基蓖麻油酸摩尔比的降低,水凝胶的生物相容性降低。当在水凝胶降解产物中孵育时,细胞的存活率最低为80%。开发了一种环境友好的合成方法,并利用可再生材料制备了新型生物相容性水凝胶。
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引用次数: 0
THE ANTI HRSV ACTIVITY OF Ferula halophila Peşmen AQUEOUS AND METHANOL EXTRACT BY MTT ASSAY MTT法测定嗜盐阿魏提取物的抗HRSV活性
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-03-22 DOI: 10.23902/TRKJNAT.805545
H. Doğan, Rüstem Duman
The trend towards natural products in the world is increasing due to the increased drug resistance of infectious diseases, the high prices for drugs and the difficulty of access. Also, bacterial or viral diseases that are difficult to treat and need a long time for proper treatment cause important infections in people. Since effective drugs could not be developed for most viral infections, it is very important to find natural products against viruses to introduce them to the world of science. The antiviral activities of the aqueous and methanol extract from Ferula halophila Pesmen, an endemic species to Turkey, has been investigated against human respiratory syncytial virus (HRSV, ATCC-VR-26). The cytotoxic and antiviral properties of plant extracts were investigated in HRSV / HEp-2 cell systems, respectively by the colorimetric MTT assay. In the study, the titer of RSV was used as 100 TCID50 (50% tissue culture infective dose). While MNTC (Maximum non-toxic concentration) of methanol extract was 195.313 µg/mL and CC50 (50% cytotoxic concentration) was 4366.22 µg/mL, it was determined as MNTC = 390.625 µg/mL and CC50 = 4366.22 for aqueous extract. According to the results, methanol extract showed more toxicity than the aqueous extract. The MNTC of Ribavirin used as a positive control was determined as 0.98 µg/mL and CC50 was 110.40 µg/mL. As a result of the study, it was determined that the methanol extract was not effective, whereas the aqueous extract had a significant anti-HRSV activity with the values of 50.69 µg/mL EC50 (50% Effective Concentration) and 97.54 Selectivity Index (SI). The EC50 and SI values of Ribavirin were 2.39 µg/mL and 46.19, respectively. According to the results, we can explain the presence of antiviral activity only in the aqueous extract and the absence in the methanol extract by the high toxicity of it and the insolubility of the antiviral compounds in the methanol extract.
由于传染病的耐药性增强、药品价格高企和难以获得,世界上使用天然产品的趋势正在增加。此外,细菌或病毒疾病难以治疗,需要很长时间才能得到适当治疗,会导致严重的感染。由于无法开发出针对大多数病毒感染的有效药物,因此找到对抗病毒的天然产品并将其引入科学界非常重要。研究了土耳其特有种嗜盐阿魏(Ferula halophila Pesmen)水提物和甲醇提物对人呼吸道合胞病毒(HRSV, ATCC-VR-26)的抗病毒活性。采用比色MTT法分别研究了植物提取物在HRSV / HEp-2细胞系统中的细胞毒和抗病毒特性。本研究采用RSV滴度为100 TCID50(50%组织培养感染剂量)。甲醇提取物的MNTC(最大无毒浓度)为195.313µg/mL, CC50(50%细胞毒浓度)为4366.22µg/mL,水提物的MNTC = 390.625µg/mL, CC50 = 4366.22。结果表明,甲醇提取物的毒性大于水提取物。阳性对照利巴韦林的MNTC为0.98µg/mL, CC50为110.40µg/mL。结果表明,甲醇提取物的抗hrsv活性不明显,而水提取物的抗hrsv活性显著,EC50(50%有效浓度)为50.69µg/mL,选择性指数(SI)为97.54。利巴韦林的EC50和SI值分别为2.39µg/mL和46.19。根据实验结果,我们可以解释为什么只有水提物有抗病毒活性,而甲醇提物没有抗病毒活性的原因是水提物的高毒性和甲醇提物中抗病毒化合物的不溶性。
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引用次数: 2
Distinctive SIRE1 Retrotransposon Patterns on Barley Chromosomes? 大麦染色体上独特的SIRE1反转录转座子模式?
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-10-09 DOI: 10.23902/trkjnat.773302
E. Karlik, N. Gozukirmizi
: SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily. Distinctive SIRE1 elements ( ENV and GAG ) distributions in barley genome were observed by using fluorescent in situ hybridization (FISH). We performed PCR to obtain tetramethylrhodamine-dUTP (TRITC)-labelled probes. Localizations of SIRE1 ENV and GAG domains were demonstrated under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed the distributions of SIRE1 elements ENV and GAG in barley genome. These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome. Özet: SIRE1 , Tyl/Copia Uzun Uç Tekrarlı (Long Terminal Repeats- LTR) retrotranspozon üst ailesine ait olan aktif, nispeten yüksek kopyalı bir retroementtir. Arpa genomundaki ayırt edici SIRE1 elementlerinin ( ENV ve GAG ) dağılımları floresan in situ hibridizasyonu (FISH) kullanılarak gözlemlendi. Tetramethylrhodamine-dUTP (TRITC)-işaretli probların elde edilmesinde PCR gerçekleştirildi. SIRE1 ENV ve GAG domainlerinin yerleşimleri, Hordeum vulgare L. cv. Hasat kök preparatlarında konfokal mikroskobu altında gösterildi. Sonuçlarımız, arpa genomundaki SIRE1 elementlerinin ENV ve GAG dağılımlarını göstermektedir. Bu sonuçlar, SIRE1 elementlerinin arpa genomunun organizasyonunun ortaya çıkarılmasına katkı sağlayacaktır.
SIRE1是一个活跃的、拷贝数相对较高的逆转录元件,属于Tyl/Copia长末端重复(LTR)逆转录转座子超家族。利用荧光原位杂交技术(FISH)观察了SIRE1基因ENV和GAG在大麦基因组中的不同分布。我们通过PCR获得TRITC标记的四甲基罗丹明探针。在共聚焦显微镜下,研究了普通葡萄球菌SIRE1 ENV和GAG结构域的定位。哈萨特根制剂。我们的结果揭示了SIRE1元件ENV和GAG在大麦基因组中的分布。这些结果可能有助于揭示大麦基因组中SIRE反转录转座子模式的组织结构。Özet: SIRE1, Tyl/Copia Uzun Uç tekrarlyi (Long Terminal Repeats- LTR)反转录转座子 st ailesine ait olan aktif, nispeten yksek kopyalyi bir retroementtir。Arpa genomundaki ayırt dici SIRE1 elementlerinin (ENV ve GAG) dağılımları floresan in situ hibridizasyonu (FISH) kullanılarak gözlemlendi。四甲基罗丹明- dutp (TRITC)- i aretli probların elde edilmesinde PCRSIRE1 envve GAG域lerinin yerle imleri, Hordeum vulgere,等。Hasat kök preparatlarında konfokal microskobu altında gösterildi。Sonuçlarımız, arpa genomundaki SIRE1 elementlerinin ENV ve GAG dağılımlarını göstermektedir。但是,相对而言,SIRE1元件在arpa基因组组织中的作用为çıkarılmasına katkki sağlayacaktır。
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引用次数: 0
USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4 桑树渣作为黑曲霉MT-4产柠檬酸底物的研究
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-10-02 DOI: 10.23902/trkjnat.670859
Rufina Aidynova, N. Arslan, M. Aydoğan
Dut posasi (DP), geleneksel bir Turk yiyecegi olan pekmez'in uretiminden sonra ortaya cikan bir atik maddedir. Bu calisma, Aspergillus niger MT-4 ile sitrik asit (SA) uretimi icin DP’nin substrat olarak kullanilabilirligini test etmek icin gerceklestirilmistir. Calismada ayrica, SA uretimini artirmak icin bazi kultur kosullari da optimize edilmistir. MP’nin nem, toplam karbonhidrat, suda-cozunur karbonhidrat, protein, lipid ve kul icerikleri sirasiyla %31,1; 47,1; 1,3; 13,4; 1,8 ve 1,4 olarak belirlenmistir. Deneyler, uretim besiyerinin 100 ml’sini iceren 250 ml'lik erlenler icerisinde gerceklestirilmistir. Hem fungal biyokutle (FB) hem de SA uretimi icin optimum DP konsantrasyonu, 120 gr/lt olarak belirlenmistir. DP bazli ortama eklenen tum KH2PO4 konsantrasyonlarinin SA uretimini azalttigi fakat FB uretimini artirdigi belirlenmistir. SA uretimi icin MgSO4 ve (NH4)2SO4’un optimal konsantrasyonlari sirasiyla 1 ve 2 gr/lt olarak bulunmustur. Diger optimal parametreler, baslangic pH'si 7,0 ve inkubasyon suresi 5 gun olarak belirlenmistir. Optimize edilmis kultur kosullari altinda, uretilen SA miktari 24,6 gr/lt olarak belirlenmistir. Besinci gunde, Yp/s; Yp/x ve Yx/s sirasiyla 0,2 gr SA/gr DP; 1,43 gr SA/gr FB ve 0,14 gr FB/gr DP olarak hesaplanmistir. DP'nin SA dahil mikrobiyal metabolitlerin uretimi icin fermentasyon substrati olarak kullanilabilirligi ilk kez bu calismada test edilmistir.
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引用次数: 4
CONSERVATION STATUS OF THREE RARE AND ENDEMIC SPECIES FROM TURKEY (KALIDIUM WAGENITZII, MUSCARI ADILII AND VERBASCUM GYPSICOLA) 土耳其3种珍稀特有物种(wagenitzii kalidium、muscari adilii和verbascum gypsicola)的保护现状
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-09-23 DOI: 10.23902/trkjnat.751851
Ali Murat Keser, G. Ayyildiz, Merve Yıldırım, A. Yaprak, G. Tug
In this study, we aimed to determine the population size and distribution areas and to re-evaluate IUCN threat categories of Kalidium wagenitzii (Aellen) Freitag & G. Kadereit, Muscari adilii M.B. Guner & H. Duman and Verbascum gypsicola Vural & Aydogdu, which are endemics of the Central Anatolia region of Turkey. The three species have limited distribution areas and high risk of extinction as a result of human impact. The perennial halophytic species K. wagenitzii was found in 5 localities around Salt Lake (Tuz Golu) and a total number of 6458 mature individuals were determined. The area of occupancy of the species is 36 km2 and the extent of occurrence is 213 km2. Uncontrolled use of water resources for agricultural purposes is a serious threat factor for the species. Kalidium wagenitzii is listed in EN category according to the IUCN Red List criteria. Muscari adilii prefers marly soils and its habitat type is formed by secondary succession after the destruction of Pinus nigra and oak forests. It is known from 3 localities around Nallihan-Beypazari with a population size of 6144 mature individuals. The area of occupancy and the extent of occurrence of the species is 12 km2 and 28 km2, respectively. The pressures on the species are road construction and increase of farmland, afforestation and factory establishment. According to the IUCN Red List criteria, the species is listed in CR category. Verbascum gypsicola is distributed on marly soils. It is known from 3 localities around Nallihan–Beypazari and one locality in Sivrihisar-Eskisehir with 2755 mature individuals in total. The area of occupancy of the species is 16 km2 and the extent of occurrence is 269 km2. Overgrazing and expansion of agricultural land, together with factory establishment are the major threats for this species, which is listed as EN in IUCN Red List.
在本研究中,我们旨在确定种群规模和分布区域,并重新评估土耳其安纳托利亚中部地区特有的Kalidium wagenitzii (Aellen) Freitag & G. Kadereit, Muscari adilii M.B. Guner & H. Duman和Verbascum gypsicola Vural & Aydogdu的IUCN威胁类别。由于人类的影响,这三个物种的分布区域有限,灭绝的风险很高。在盐湖(Tuz Golu)周围的5个地点发现了多年生盐生植物wagenitzi,共测定了6458个成熟个体。该物种的占用面积为36 km2,发生范围为213 km2。不受控制的农业用水是该物种的一个严重威胁因素。根据世界自然保护联盟红色名录标准,瓦格尼茨钾被列为EN类。黑松林和栎林被破坏后,其生境类型由次生演替形成。在Nallihan-Beypazari周围的3个地点已知,种群大小为6144只成熟个体。该物种的占用面积为12 km2,发生范围为28 km2。道路建设和农田、造林、工厂的增加对该物种造成了压力。根据世界自然保护联盟的红色名录标准,该物种被列为CR类。石膏菊分布在沼泽土壤上。在Nallihan-Beypazari周围的3个地方和Sivrihisar-Eskisehir的一个地方已知,共有2755只成熟个体。该物种的占用面积为16 km2,发生范围为269 km2。过度放牧和农业用地扩张以及工厂建设是对该物种的主要威胁,已被列入IUCN红色名录EN。
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引用次数: 2
THE COMMERCIAL AND DISCARD CATCH RATES OF THE TRAWL FISHERY IN THE ISKENDERUN BAY (NORTHEASTERN LEVANTINE SEA) 伊斯肯德伦湾(东北黎凡特海)拖网渔业的商业捕捞率和废弃捕捞率
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-09-18 DOI: 10.23902/TRKJNAT.773435
C. Dalyan
Deniz kaynaklarinin surdurulebilirligi icin balikcilik yonetimi son derece onemlidir. Bu nedenle balikcilik alanlari surekli olarak izlenmelidir. Bu calismada kullanilan veriler Iskenderun Korfezi'nde (Kuzeydogu Levant Denizi) avlanan bir ticari trol teknesi ile toplanmistir. Balikciliga bagli veriler, 2012-2013 balikcilik sezonunda tekne murettebat yardimi ile kaydedilmistir. 26 saat suren 33 trol cekimi 44 mm rombik ag gozu boyutuna sahip torba kullanilarak gerceklestirilmistir. Trol cekim hizi 2,5 ila 3,0 knot arasinda degismis ve en fazla 70 dakika surmustur. Iskenderun Korfezi’nde 39-69 m derinlik konturu incelenmistir. 32 tur iskarta olarak degerlendirilirken, 35 tur ticari av icinde yer almistir. Buna karsin, toplam av CPUEW (birim zamanda elde edilen biyokutle) acisindan degerlendirildiginde %67,2’sinin ticari ava %32,8'inin ise iskarta ait oldugu saptanmistir.
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引用次数: 3
NOTES ON LEAF AND STEM ANATOMY OF Thlaspi Sensu Lato 标题海苔叶茎解剖注释
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-09-16 DOI: 10.23902/TRKJNAT.762509
Cengiz Karaismailoğlu, Osman Erol
In this study, anatomical characteristics of leaves and stems of 19 taxa of Thlaspi sensu lato, 9 of which are endemic to Turkey, were investigated in order to determine taxonomic relationships among the studied taxa. The anatomical characteristics of all taxa were determined and assessed using the cluster analysis and the principal component analysis. The transverse sections of the leaves and stems exhibited various diagnostic characters in terms of the pattern of epidermal cell and mesophyll layers, the number and size of vascular bundles, and the thickness of the cortex and endodermis. Important differences were detected in number, size, and index of stomata, and in epidermal cell wall structures in the adaxial and abaxial surfaces. The results showed that the compared anatomical characteristics among taxa are partially compatible with their sectional delimitation in their traditional rank in The Flora of Turkey and the East Aegean Islands. The results also point out that some taxonomic re-arrangements may be required.
本文对土耳其特有的19个树属(Thlaspi sensu lato)的叶和茎的解剖特征进行了研究,以确定不同分类群之间的分类关系。利用聚类分析和主成分分析对各分类群的解剖特征进行了分析和评价。叶片和茎的横切面在表皮细胞和叶肉层的形态、维管束的数量和大小、皮层和内胚层的厚度等方面表现出不同的诊断特征。气孔的数量、大小、指数以及近、背面表皮细胞壁结构均存在显著差异。结果表明,《土耳其植物志》和《东爱琴海群岛植物志》中各分类群的比较解剖特征与它们在传统等级上的剖面划分部分一致。结果还指出,可能需要进行一些分类上的重新安排。
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引用次数: 3
AGE, GROWTH AND OTOLITH BIOMETRY-BODY LENGTH RELATIONSHIPS OF RED BANDFISH (Cepola macrophthalma L., 1758) IN THE SEA OF MARMARA, TURKEY 土耳其马尔马拉海红带鱼(Cepola macrophthalma L., 1758)的年龄、生长和耳石生物特征-体长关系
IF 0.7 Q4 MULTIDISCIPLINARY SCIENCES Pub Date : 2020-09-11 DOI: 10.23902/trkjnat.714201
A. İşmen, M. A. İhsanoğlu, I. B. Daban, H. Inceoğlu
Cepola macrophthalma (Linnaeus, 1758)’in yas, buyume ve otolit biyometri-total boy iliskileri arastirilmistir. Bireyler Mart 2012-Haziran 2014 tarihleri arasinda Marmara Denizi'nde algarna ile yakalanmistir. Bu calismada orneklenen 51,5 cm'lik C. macrophthalma bireyinin boyu, Marmara Denizi'nde bu tur icin maksimum olarak kaydedilmistir. Boy-agirlik iliskisi denklemine gore buyume tipi negatif allometrik olarak belirlenmistir (b=1,36). Otolith boyu-otolith agirligi, otolith genisligi- otolith agirligi, otolith boyu-total boy, otolith genisligi-total boy, otolit boyu-otolit genisligi, total boy-otolit agirligi iliskileri OW=0,0002*OL2,6377 (R2=0,91), OW=0,001*OWi2,6215 (R2=0,94), OL=0,057*TL+3,2087 (R2=0,57), OWi=0,0316*TL+1,8511 (R2=0,55), OWi=0,527*OL+0,2015 (R2=0,86) OW=0,0004*TL+0,0029 (R2=0,58) olarak hesaplanmistir. Yaslar 80 adet otolit okumasindan hesaplanmistir; gozlemlenen en kucuk ve en buyuk yaslar sirasiyla 1 ve 5’tir. von Bertalanffy denklemine gore hesaplanan buyume parametreleri degerleri L∞=61,95 cm, K=0,19 yil-1, t0 = -0,05 yil olarak belirlenmistir. Stogun buyuk cogunlugu (%77) genc bireylerden (1-2 yas grubu) olusmaktadir. Marmara Denizi’nde C. macrophthalma stogunun daha cok genc bireylerden olusmasi stoklarin av baskisi altinda oldugunu dusundurmektedir.
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引用次数: 0
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Trakya University Journal of Natural Sciences
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