Pub Date : 2022-07-14DOI: 10.23902/trkjnat.1113991
E. C. Fi̇dan, Ü. Sirin
Despite Anatolia's rich biodiversity potential, the blackfly fauna of Turkey has not been fully explored. 15 blackfly species from 73 different lotic habitats from the Western Black Sea Region have been reported. One species, Simulium (Nevermannia) carthusiense Grenier & Dorier, 1959 is new for the Turkish fauna. 10 species have been recorded from this region for the first time. The material was mainly collected in the spring from 2016 to 2018. Distributional remarks both in Turkey and world are given along with brief taxonomic evaluations for each species.
{"title":"CONTRIBUTIONS TO THE BLACK FLY FAUNA (DIPTERA, SIMULIIDAE) OF TURKEY WITH A NEW RECORD FROM THE WESTERN BLACK SEA REGION","authors":"E. C. Fi̇dan, Ü. Sirin","doi":"10.23902/trkjnat.1113991","DOIUrl":"https://doi.org/10.23902/trkjnat.1113991","url":null,"abstract":"Despite Anatolia's rich biodiversity potential, the blackfly fauna of Turkey has not been fully explored. 15 blackfly species from 73 different lotic habitats from the Western Black Sea Region have been reported. One species, Simulium (Nevermannia) carthusiense Grenier & Dorier, 1959 is new for the Turkish fauna. 10 species have been recorded from this region for the first time. The material was mainly collected in the spring from 2016 to 2018. Distributional remarks both in Turkey and world are given along with brief taxonomic evaluations for each species.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"44 24 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78792366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-12DOI: 10.23902/trkjnat.1123152
S. Ülgentürk, Cansu Ercan, B. Yaşar, M. Kaydan
Coccoidea üstfamilyası (Hemiptera: Sternorryncha: Coccomorpha) tüm dünyada birçok zararlı türe sahiptir. Türkiye’de bulunan coccidlerin control listesi en son 2013 tarihinde yayınlanmış olup liste 12 familyadan 359 tür içermiştir. Bu çalışmada kontrol listesi 18 farklı familyaya bağlı 134 cins içerisinde bulunan 359 tür olacak şekilde güncellenmiştir. Bu çalışmada 20 familyaya bağlı 149 cins içerisinde bulunan 401 tür olacak şekilde güncellenmektedir. Türkiye’de bulunan coccoidlerin çoğunluğu Palaearktik yayılım göstermektedir. Tür sayısı bakımından Diaspididae familyası 46 cinse bağlı 122 tür ile en çok türü barındıran familya iken bunu 40 cinsten 119 tür ile Pseudococcidae familyası ve 29 cinse ait 71 tür ile Coccidae familyası takip etmektedir. Diğer familyalar Acanthococcidae (11 cins, 30 tür), Eriococcidae (1 cins, 1 tür), Asterolecaniidae (4 cins, 12 tür), Kermesidae (2 cins, 12 tür), Margarodidae (2 cins, 9 tür), Cryptococcidae (1 cins, 1 tür), Monophlebidae (3 cins, 3 tür), Matsucoccidae (1 cins, 2 tür), Putoidae (1 cins, 4 tür), Rhizoecidae (1 cins, 4 tür), Cerococcidae (1 cins, 2 tür), Leconodiaspidae (1 cins, 1 tür), Dactylopiidae (1 cins, 1 tür), Marchalinidae (1 cins, 2 tür), Micrococcidae (1 cins, 1 tür) Ortheziidae ve (1 cins, 3 tür) Phoenicococcidae (1 cins, 1 tür). olarak listelenmiştir.
{"title":"Checklist of Turkish Scale Insect (Hemiptera: Sternorryncha: Coccomorpha) Species","authors":"S. Ülgentürk, Cansu Ercan, B. Yaşar, M. Kaydan","doi":"10.23902/trkjnat.1123152","DOIUrl":"https://doi.org/10.23902/trkjnat.1123152","url":null,"abstract":"Coccoidea üstfamilyası (Hemiptera: Sternorryncha: Coccomorpha) tüm dünyada birçok zararlı türe sahiptir. Türkiye’de bulunan coccidlerin control listesi en son 2013 tarihinde yayınlanmış olup liste 12 familyadan 359 tür içermiştir. Bu çalışmada kontrol listesi 18 farklı familyaya bağlı 134 cins içerisinde bulunan 359 tür olacak şekilde güncellenmiştir. Bu çalışmada 20 familyaya bağlı 149 cins içerisinde bulunan 401 tür olacak şekilde güncellenmektedir. Türkiye’de bulunan coccoidlerin çoğunluğu Palaearktik yayılım göstermektedir. Tür sayısı bakımından Diaspididae familyası 46 cinse bağlı 122 tür ile en çok türü barındıran familya iken bunu 40 cinsten 119 tür ile Pseudococcidae familyası ve 29 cinse ait 71 tür ile Coccidae familyası takip etmektedir. Diğer familyalar Acanthococcidae (11 cins, 30 tür), Eriococcidae (1 cins, 1 tür), Asterolecaniidae (4 cins, 12 tür), Kermesidae (2 cins, 12 tür), Margarodidae (2 cins, 9 tür), Cryptococcidae (1 cins, 1 tür), Monophlebidae (3 cins, 3 tür), Matsucoccidae (1 cins, 2 tür), Putoidae (1 cins, 4 tür), Rhizoecidae (1 cins, 4 tür), Cerococcidae (1 cins, 2 tür), Leconodiaspidae (1 cins, 1 tür), Dactylopiidae (1 cins, 1 tür), Marchalinidae (1 cins, 2 tür), Micrococcidae (1 cins, 1 tür) Ortheziidae ve (1 cins, 3 tür) Phoenicococcidae (1 cins, 1 tür). olarak listelenmiştir.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"42 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87974494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-08DOI: 10.23902/trkjnat.1115893
F. Can, B. Ulasli, Evrim Karaçetin, Serdar Akar
The aim of this article is to contribute to the recent distribution and systematic classification of Zygaenidae (Lepidoptera) in 12 provinces in the Aegean, eastern Anatolia and Thrace regions of Turkey, with the records obtained in 2020 and 2021. Twenty Zygaenidae species from five genera belonging to Procridinae and Zygaeninae subfamilies were recorded: Theresimima Strand, 1917 (1 species), Rhagades Wallengren, 1863 (1 species), Adscita Retzius, 1783 (1 species), Jordanita Verity, 1946 (5 species), and Zygaena Fabricius, 1775 (12 species). It is the first record of Zygaena (Zygaena) ephialtes (Linnaeus, 1767) in Thrace region of Turkey since Herrich-Schäffer (1845). Zygaena (Mesembrynus) lydia Staudinger, 1887 endemic to Turkey, was also recently recorded in this study. In addition, Zygaena (Agrumenia) armena Eversman, 1851, which is known only in Turkey and Georgia, was also recorded from the province of Ardahan in the eastern Anatolia region of Turkey.
{"title":"CONTRIBUTION TO THE KNOWLEDGE OF THE ZYGAENIDAE (LEPIDOPTERA) OF TURKEY","authors":"F. Can, B. Ulasli, Evrim Karaçetin, Serdar Akar","doi":"10.23902/trkjnat.1115893","DOIUrl":"https://doi.org/10.23902/trkjnat.1115893","url":null,"abstract":"The aim of this article is to contribute to the recent distribution and systematic classification of Zygaenidae (Lepidoptera) in 12 provinces in the Aegean, eastern Anatolia and Thrace regions of Turkey, with the records obtained in 2020 and 2021. Twenty Zygaenidae species from five genera belonging to Procridinae and Zygaeninae subfamilies were recorded: Theresimima Strand, 1917 (1 species), Rhagades Wallengren, 1863 (1 species), Adscita Retzius, 1783 (1 species), Jordanita Verity, 1946 (5 species), and Zygaena Fabricius, 1775 (12 species). It is the first record of Zygaena (Zygaena) ephialtes (Linnaeus, 1767) in Thrace region of Turkey since Herrich-Schäffer (1845). Zygaena (Mesembrynus) lydia Staudinger, 1887 endemic to Turkey, was also recently recorded in this study. In addition, Zygaena (Agrumenia) armena Eversman, 1851, which is known only in Turkey and Georgia, was also recorded from the province of Ardahan in the eastern Anatolia region of Turkey.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75620684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-07DOI: 10.23902/trkjnat.1106928
Zafer Şakacı, Y. Camlitepe
Mosquitoes are the most important vectors that can transmit many diseases agents to humans and animals. This study was conducted in Edirne central district between July 2017 and July 2018 to identify important vector mosquito species, determine their seasonality and distribution pattern in general terms. Larvae, pupae, and adults were collected from areas assessed as being particularly suitable for medically important species included in the genus of Aedes, Culex, and Anopheles. In addition to the foci naturally found in the areas, ovitraps placed in the proper points were also used. As a result, 3155 female and 353 male mosquitoes belonging to 13 species of 5 genera were obtained. Among these species, Anopheles sacharovi (the primary vector of malaria in Turkey) and Culex pipiens s.l. (the primary vector of West Nile Fever) has been recognized as a public health threat to the province. An. sacharovi was represented at a very low population level, while Cx. pipiens s.l. was determined as the most common and dense species in the study area. Known to have a high preference for warmer climate compared to members of the Anopheles maculipennis s.l., An. sacharovi has the risk of increasing its population in the region with possible global warming in the future. The importance of this risk increases even more since rice production is widespread especially in Edirne and this species can use the paddy fields as an effective breeding place. While Aedes caspius was commonly encountered, Ae. albopictus was not found during the field observation and ovitrap controls.
{"title":"A MOSQUITO SURVEY OF CULICIDAE SPECIES AT EDİRNE CENTRAL DISTRICT FOR DISEASE VECTOR","authors":"Zafer Şakacı, Y. Camlitepe","doi":"10.23902/trkjnat.1106928","DOIUrl":"https://doi.org/10.23902/trkjnat.1106928","url":null,"abstract":"Mosquitoes are the most important vectors that can transmit many diseases agents to humans and animals. This study was conducted in Edirne central district between July 2017 and July 2018 to identify important vector mosquito species, determine their seasonality and distribution pattern in general terms. Larvae, pupae, and adults were collected from areas assessed as being particularly suitable for medically important species included in the genus of Aedes, Culex, and Anopheles. In addition to the foci naturally found in the areas, ovitraps placed in the proper points were also used. As a result, 3155 female and 353 male mosquitoes belonging to 13 species of 5 genera were obtained. Among these species, Anopheles sacharovi (the primary vector of malaria in Turkey) and Culex pipiens s.l. (the primary vector of West Nile Fever) has been recognized as a public health threat to the province. An. sacharovi was represented at a very low population level, while Cx. pipiens s.l. was determined as the most common and dense species in the study area. Known to have a high preference for warmer climate compared to members of the Anopheles maculipennis s.l., An. sacharovi has the risk of increasing its population in the region with possible global warming in the future. The importance of this risk increases even more since rice production is widespread especially in Edirne and this species can use the paddy fields as an effective breeding place. While Aedes caspius was commonly encountered, Ae. albopictus was not found during the field observation and ovitrap controls.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"6 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72569807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-05DOI: 10.23902/trkjnat.1124483
M. Fent, A. Dursun
In this study, an up-to-date list of Pentatomidae family, one of the large families of Heteroptera suborder, in Turkey with the latest taxonomic developments is presented. As a result of the literature review, 61 genera and 174 species/subspecies (2 species only in European Turkey, 97 species only in Asian Turkey) belonging to 4 subfamilies and 13 tribes from the Turkish Pentatomidae fauna were determined. Distribution of the species by subfamilies: 14 species of Asopinae (10 in European and 13 in Asian Turkey), 125 species of Pentatominae (57 in European and 125 in Asian Turkey), Phyllocephalinae 1 species (only in Asian Turkey) and 34 species Podopinae (10 in European and 33 in Asian Turkey). The distributions of these species in Turkey (in European and Asian Turkey), Palaearctic distributions and changes in their taxonomic positions are given. In addition, additional records for 53 species, mainly from the Thrace Region, were given from various localities in Thrace and Anatolia. Pentatoma rufipes (Linnaeus, 1758) is first record for the Thrace Region. Four species, which were given in 19. century without a specific locality record, need to be verified. Two species were excluded from the Turkey list.
{"title":"An up-to-date checklist of Turkish Pentatomidae (Hemiptera: Heteroptera) with additional records","authors":"M. Fent, A. Dursun","doi":"10.23902/trkjnat.1124483","DOIUrl":"https://doi.org/10.23902/trkjnat.1124483","url":null,"abstract":"In this study, an up-to-date list of Pentatomidae family, one of the large families of Heteroptera suborder, in Turkey with the latest taxonomic developments is presented. As a result of the literature review, 61 genera and 174 species/subspecies (2 species only in European Turkey, 97 species only in Asian Turkey) belonging to 4 subfamilies and 13 tribes from the Turkish Pentatomidae fauna were determined. Distribution of the species by subfamilies: 14 species of Asopinae (10 in European and 13 in Asian Turkey), 125 species of Pentatominae (57 in European and 125 in Asian Turkey), Phyllocephalinae 1 species (only in Asian Turkey) and 34 species Podopinae (10 in European and 33 in Asian Turkey). The distributions of these species in Turkey (in European and Asian Turkey), Palaearctic distributions and changes in their taxonomic positions are given. In addition, additional records for 53 species, mainly from the Thrace Region, were given from various localities in Thrace and Anatolia. Pentatoma rufipes (Linnaeus, 1758) is first record for the Thrace Region. Four species, which were given in 19. century without a specific locality record, need to be verified. Two species were excluded from the Turkey list.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89962971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-03DOI: 10.23902/trkjnat.1106190
S. Anlaş
In the study, a new species of the genus Leptobium Casey from central northern Anatolia in Turkey is described, illustrated, and distinguished from congeners: Leptobium orgeli sp. n. (Çorum province). Distributions of the endemic Leptobium species in central northern Anatolia are mapped. Additional records of five species of Leptobium from Turkey are presented. A total of 21 species is now known from Turkey.
在这项研究中，描述了土耳其安纳托利亚中北部的Leptobium Casey属的一个新种，并说明了它与同属植物的区别:Leptobium orgeli sp. n. (Çorum省)。绘制了安那托利亚中北部特有的细鳞菌的分布图。介绍了土耳其五种细鳞菌的附加记录。目前已知的土耳其共有21种。
{"title":"A new species of the genus Leptobium Casey (Coleoptera: Staphylinidae: Paederinae) from central northern Anatolia, and a distributional checklist to Turkish species","authors":"S. Anlaş","doi":"10.23902/trkjnat.1106190","DOIUrl":"https://doi.org/10.23902/trkjnat.1106190","url":null,"abstract":"In the study, a new species of the genus Leptobium Casey from central northern Anatolia in Turkey is described, illustrated, and distinguished from congeners: Leptobium orgeli sp. n. (Çorum province). Distributions of the endemic Leptobium species in central northern Anatolia are mapped. Additional records of five species of Leptobium from Turkey are presented. A total of 21 species is now known from Turkey.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"166 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77559722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-03DOI: 10.23902/trkjnat.1110724
Ş. Kök, I. Özdemir
This study aims to reveal important results on the relationship and the variation between the number of aphid species and the number of invasive alien aphid species in Turkey, the taxonomic diversity of invasive alien aphids, the distribution in Turkey and the regions of origin of invasive alien aphids. The comprehensive study present the presence of 60 invasive alien aphid species belonging to 35 genus from the Aphidomorpha suborder (Hemiptera) recorded in Turkey between 1903 and 2021. It was determined that the invasive alien aphid constitute about 10% of the aphid fauna of Turkey including 592 species belonging to 148 genera. Based on 119 years of data, the average introduction rate of invasive alien aphids into the aphid fauna of Turkey was calculated as 0.51 species per year. Also, it was determined that the invasive alien aphids are distributed in 60 of 81 provinces in Turkey. This study is the first comprehensive research including important data on invasive alien aphids in Turkey. As a result, it is thought that more local and territorial studies should be carried out in order to determine detally the current status and distribution of invasive alien aphids in Turkey.
{"title":"INVASIVE ALIEN APHIDS (HEMIPTERA: APHIDOMORPHA) OF TURKEY","authors":"Ş. Kök, I. Özdemir","doi":"10.23902/trkjnat.1110724","DOIUrl":"https://doi.org/10.23902/trkjnat.1110724","url":null,"abstract":"This study aims to reveal important results on the relationship and the variation between the number of aphid species and the number of invasive alien aphid species in Turkey, the taxonomic diversity of invasive alien aphids, the distribution in Turkey and the regions of origin of invasive alien aphids. The comprehensive study present the presence of 60 invasive alien aphid species belonging to 35 genus from the Aphidomorpha suborder (Hemiptera) recorded in Turkey between 1903 and 2021. It was determined that the invasive alien aphid constitute about 10% of the aphid fauna of Turkey including 592 species belonging to 148 genera. Based on 119 years of data, the average introduction rate of invasive alien aphids into the aphid fauna of Turkey was calculated as 0.51 species per year. Also, it was determined that the invasive alien aphids are distributed in 60 of 81 provinces in Turkey. This study is the first comprehensive research including important data on invasive alien aphids in Turkey. As a result, it is thought that more local and territorial studies should be carried out in order to determine detally the current status and distribution of invasive alien aphids in Turkey.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82892406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.23902/trkjnat.1103047
A. Üstün, Serkan Örtucu
In this study, fluconazole(FLZ) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles(NPs) were prepared with two different formulations consisting of polyvinyl alcohol(PVA) and PVA-rhamnolipid(R) in order to improve antibiofilm activity against Candida albicans ATCC 90028. The encapsulation efficiency, drug loading capacity, in-vitro release, characterization and antibiofilm activity of these formulations were compared. Characterization of NPs were analyzed by scanning electron microscopy(SEM) and Zetasizer. Drug loading capacity and encapsulation efficiency percentages were measured by spectrophotometric method. PLGA-NPs were spherical in shape with mean sizes of ~300 nm and surface charge of FLZ loaded PVA and PVA-R-PLGA NPs -25,9±1.99, -48,1±2.46, respectively. Sustained release of FLZ (≥60% after 6 h) were obtained in PVA-R PLGA-NPs. The encapsulation efficiency percentages of PVA-FLZ-PLGA and PVA-R-FLZ-PLGA were 50% and %85 respectively. Antibiofilm inhibition percentages are 55% and 63% respectively. These results show that the PVA-R-FLZ-PLGA drug delivery system is a new therapeutic approach that can be used in infections caused by C.albicans.
本研究以聚乙烯醇(PVA)和PVA-鼠李糖脂(R)两种不同的配方制备了负载氟康唑(FLZ)的聚乳酸-羟基乙酸(PLGA)纳米颗粒(NPs)，以提高其对白色念珠菌ATCC 90028的抗菌膜活性。比较了这些制剂的包封效率、载药量、体外释放度、表征及抗生物膜活性。利用扫描电子显微镜(SEM)和Zetasizer分析了NPs的表征。用分光光度法测定载药量和包封率。PLGA-NPs呈球形，平均尺寸为~300 nm, FLZ负载的PVA和PVA- r - plga NPs的表面电荷分别为-25、9±1.99、-48、1±2.46。PVA-R PLGA-NPs中FLZ的缓释(6 h后≥60%)。PVA-FLZ-PLGA和PVA-R-FLZ-PLGA的包封率分别为50%和85%。抗菌膜抑制率分别为55%和63%。这些结果表明PVA-R-FLZ-PLGA给药系统是一种新的治疗白色念珠菌感染的方法。
{"title":"EVALUATION OF THE ANTIBIOFILM EFFECT OF FLUCONAZOLE LOADED PLGA NANOPARTICLES PREPARED USING RHAMNOLIPID","authors":"A. Üstün, Serkan Örtucu","doi":"10.23902/trkjnat.1103047","DOIUrl":"https://doi.org/10.23902/trkjnat.1103047","url":null,"abstract":"In this study, fluconazole(FLZ) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles(NPs) were prepared with two different formulations consisting of polyvinyl alcohol(PVA) and PVA-rhamnolipid(R) in order to improve antibiofilm activity against Candida albicans ATCC 90028. The encapsulation efficiency, drug loading capacity, in-vitro release, characterization and antibiofilm activity of these formulations were compared. Characterization of NPs were analyzed by scanning electron microscopy(SEM) and Zetasizer. Drug loading capacity and encapsulation efficiency percentages were measured by spectrophotometric method. PLGA-NPs were spherical in shape with mean sizes of ~300 nm and surface charge of FLZ loaded PVA and PVA-R-PLGA NPs -25,9±1.99, -48,1±2.46, respectively. Sustained release of FLZ (≥60% after 6 h) were obtained in PVA-R PLGA-NPs. The encapsulation efficiency percentages of PVA-FLZ-PLGA and PVA-R-FLZ-PLGA were 50% and %85 respectively. Antibiofilm inhibition percentages are 55% and 63% respectively. These results show that the PVA-R-FLZ-PLGA drug delivery system is a new therapeutic approach that can be used in infections caused by C.albicans.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"AES-10 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84517253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-17DOI: 10.23902/trkjnat.1085192
E. Demirkan, Elif Ertürk, G. Yıldız, Tuba Sevgi̇, A. Aybey
In this study, different parts of Phytolacca americana L. (Pokeweed) fruit from Turkey were investigated for their antioxidant, antibacterial, antibiofilm and anticancer potentials. To appraise of the antioxidant potentials the radical scavenging activities, reducing power and total phenolic content were determined. The antibacterial and antibiofilm activities of the extract against Enterococcus faecalis, Yersinia enterocolitica, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Salmonella typhimurium were evaluated by using agar-well diffusion, minimum inhibitory concentration (MIC) and biofilm inhibitory concentration (BIC) assays. In addition of latening the onset of apoptosis depending on dose, the potential of the anti-proliferative effects were investigated on MDA-MB-231 and MCF-7 cells. The highest free radical scavenging activity and phenolic content were found in seed extract of P. americana L. Seed extract showed the highest inhibition zones and showed good antibacterial activity at 2.5-5 mg/ml MIC concentrations against all bacterial strains. More significantly, seed extract was found effective on inhibition of early phase biofilm formation at 2.5- 10 mg/ml BIC concentrations against all used bacterial strains. In addition, only seed extract exhibited strong antigrowth effects on cells at different concentrations for 48 and 72 h. Subsequently, the main mechanisms of cell death of seed extract in MDA-MB-231 and MCF-7 cells were investigated led to late stage of apoptosis in the cells that cell showed positivity both Hoechst 33342 and PI at the dose dependent manner. This study showed that P. americana seed extract can contribute to alternative medicine studies and have potential power in the pharmaceutical industry.
{"title":"IN VITRO EVALUATIONS OF ANTIOXIDANT, ANTIMICROBIAL AND ANTICANCER POTENTIAL OF Phytolacca americana L. (POKEWEED) SEED EXTRACT","authors":"E. Demirkan, Elif Ertürk, G. Yıldız, Tuba Sevgi̇, A. Aybey","doi":"10.23902/trkjnat.1085192","DOIUrl":"https://doi.org/10.23902/trkjnat.1085192","url":null,"abstract":"In this study, different parts of Phytolacca americana L. (Pokeweed) fruit from Turkey were investigated for their antioxidant, antibacterial, antibiofilm and anticancer potentials. To appraise of the antioxidant potentials the radical scavenging activities, reducing power and total phenolic content were determined. The antibacterial and antibiofilm activities of the extract against Enterococcus faecalis, Yersinia enterocolitica, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Salmonella typhimurium were evaluated by using agar-well diffusion, minimum inhibitory concentration (MIC) and biofilm inhibitory concentration (BIC) assays. In addition of latening the onset of apoptosis depending on dose, the potential of the anti-proliferative effects were investigated on MDA-MB-231 and MCF-7 cells. The highest free radical scavenging activity and phenolic content were found in seed extract of P. americana L. Seed extract showed the highest inhibition zones and showed good antibacterial activity at 2.5-5 mg/ml MIC concentrations against all bacterial strains. More significantly, seed extract was found effective on inhibition of early phase biofilm formation at 2.5- 10 mg/ml BIC concentrations against all used bacterial strains. In addition, only seed extract exhibited strong antigrowth effects on cells at different concentrations for 48 and 72 h. Subsequently, the main mechanisms of cell death of seed extract in MDA-MB-231 and MCF-7 cells were investigated led to late stage of apoptosis in the cells that cell showed positivity both Hoechst 33342 and PI at the dose dependent manner. This study showed that P. americana seed extract can contribute to alternative medicine studies and have potential power in the pharmaceutical industry.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74065040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-24DOI: 10.23902/trkjnat.1082308
F. Turgut
This study was carried out in May-October 2013 and May-November 2017 in Sinop Province (Western Black Sea Region-Turkey). The Culicoides Latreille biting midges were collected with light traps and mounted on microscope slides in phenol-Canada balsam. The basic morphological features of Culicoides clastrieri Callot, Kremer & Deduit, 1962 and Culicoides tbilisicus Dzhafarov, 1964 in Sinop (Turkey) province were studied. Culicoides clastrieri is recorded for the first time from Turkey. Thus, the number of Culicoides species in Turkey reached to 72. In addition, the first taxonomic measurements and data of males and females of the poorly-known C. tbilisicus, whose distribution in Turkey was reported recently, were evaluated.
{"title":"MORPHOLOGICAL CHARACTERISTICS OF TWO Culicoides Latreille BITING MIDGES (DIPTERA, CERATOPOGONIDAE) WITH A NEW RECORD FOR TURKISH FAUNA","authors":"F. Turgut","doi":"10.23902/trkjnat.1082308","DOIUrl":"https://doi.org/10.23902/trkjnat.1082308","url":null,"abstract":"This study was carried out in May-October 2013 and May-November 2017 in Sinop Province (Western Black Sea Region-Turkey). The Culicoides Latreille biting midges were collected with light traps and mounted on microscope slides in phenol-Canada balsam. The basic morphological features of Culicoides clastrieri Callot, Kremer & Deduit, 1962 and Culicoides tbilisicus Dzhafarov, 1964 in Sinop (Turkey) province were studied. Culicoides clastrieri is recorded for the first time from Turkey. Thus, the number of Culicoides species in Turkey reached to 72. In addition, the first taxonomic measurements and data of males and females of the poorly-known C. tbilisicus, whose distribution in Turkey was reported recently, were evaluated.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"27 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78734912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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