Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609979
Boonrasri Seeleang, S. Ongwattanakul, C. Promptmas, S. Boonruang, W. Mohammed, R. Jolivot
An alternative optical label-free sensor, Guided Mode Resonance (GMR), is demonstrated in this paper for detection of glycated hemoglobin (HbA1c). The GMR sensor surface is modified by cross-linking with 3-amino phenylboronic acids (3-APBA) using 3-(glycidyloxypropyl) trimethoxysilane (GOPTMS). 3-APBA provides a specific interaction with HbA1c under alkaline condition. The sensing scheme is performed by tracking a peak resonance in a reflection spectrum. That relies on changes in refractive index on the sensor’s surface. Percent of HbA1c in total hemoglobin can be estimated using a ration of resonance shift due to absorbed HbA1c and the shift from changes in a bulk refractive index of a whole blood (ΔλHbA1c/ΔλTotalHb). In the measurement, Lypochek diabetes controls having 5.1% (range: 4.6–5.6% measured by HPLC) and 9.5% (range: 8.5–10.4% measured by HPLC) HbA1c are used. The results from a resonance shift ration is linearly proportional to the measurement from the HPLC standard method with a precision of 7.71–20.77% CV. This guarantees boronate-modified GMR affinity sensor for the monitoring of glycemic status in diabetes management.
{"title":"Boronic acid Functionalized Guided Mode Resonance Sensor for HbA1c Detection","authors":"Boonrasri Seeleang, S. Ongwattanakul, C. Promptmas, S. Boonruang, W. Mohammed, R. Jolivot","doi":"10.1109/BMEICON.2018.8609979","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609979","url":null,"abstract":"An alternative optical label-free sensor, Guided Mode Resonance (GMR), is demonstrated in this paper for detection of glycated hemoglobin (HbA1c). The GMR sensor surface is modified by cross-linking with 3-amino phenylboronic acids (3-APBA) using 3-(glycidyloxypropyl) trimethoxysilane (GOPTMS). 3-APBA provides a specific interaction with HbA1c under alkaline condition. The sensing scheme is performed by tracking a peak resonance in a reflection spectrum. That relies on changes in refractive index on the sensor’s surface. Percent of HbA1c in total hemoglobin can be estimated using a ration of resonance shift due to absorbed HbA1c and the shift from changes in a bulk refractive index of a whole blood (ΔλHbA1c/ΔλTotalHb). In the measurement, Lypochek diabetes controls having 5.1% (range: 4.6–5.6% measured by HPLC) and 9.5% (range: 8.5–10.4% measured by HPLC) HbA1c are used. The results from a resonance shift ration is linearly proportional to the measurement from the HPLC standard method with a precision of 7.71–20.77% CV. This guarantees boronate-modified GMR affinity sensor for the monitoring of glycemic status in diabetes management.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133248922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609927
Y. Iwata, K. Fukuda, M. Fujii
Herein, we introduce a depth-selective filter to our recently developed equilateral-triangle probe arrangement to suppress the influence of skin-blood-flow changes on cerebral-blood flow in near-infrared spectroscopy (NIRS) brain-function measurements. The effect of the filter was evaluated by both simulations and experiments. According to the simulations, the influence of the absorption-coefficient perturbations in the superficial layer below the triangle’s vertex can be successfully reduced using the filter. We found that applying the new filter process to experiments performed using a system containing an equilateral-triangle sensor arrangement can help in successfully reducing the influence of blood-flow variations accompanying postural changes. Thus, it is valuable to obtain topographic images using this depth-selective filter, and the equilateral-triangle sensor arrangement is suitable for enhancing the filter effect.
{"title":"Suppression of superficial hemodynamic changes using a depth-selective filter in near-infrared spectroscopy measurements","authors":"Y. Iwata, K. Fukuda, M. Fujii","doi":"10.1109/BMEICON.2018.8609927","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609927","url":null,"abstract":"Herein, we introduce a depth-selective filter to our recently developed equilateral-triangle probe arrangement to suppress the influence of skin-blood-flow changes on cerebral-blood flow in near-infrared spectroscopy (NIRS) brain-function measurements. The effect of the filter was evaluated by both simulations and experiments. According to the simulations, the influence of the absorption-coefficient perturbations in the superficial layer below the triangle’s vertex can be successfully reduced using the filter. We found that applying the new filter process to experiments performed using a system containing an equilateral-triangle sensor arrangement can help in successfully reducing the influence of blood-flow variations accompanying postural changes. Thus, it is valuable to obtain topographic images using this depth-selective filter, and the equilateral-triangle sensor arrangement is suitable for enhancing the filter effect.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130916120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study is to measure a floor reacting force by the wearable device. We attached six pressure sensors under a sole and created the estimating equation of floor reacting force using the sensor outputs by the data stepping on a force plate. Estimation was tried by the stepping motion and a normal gait. We tried four types of estimating equation: multiple coefficients or a single coefficient and individual or general, respectively. As a result, the errors were minimum during the expecting motion with multiple and individual coefficients. However, the errors increased too much during unexpected motions. In case of such motions, estimation with a single coefficient would rather better. Moreover, using the general estimating equation for any test subjects, estimation with multiple coefficients could not reduce the errors much relative to the case with a single coefficient. These results indicated that using many explanatory variables might be good for a limited condition, however, a few variables might be enough for covering a wide range of situations, motions or test subjects. After all, using a generalized estimating equation, the estimation error was about 20kgf on average, which is too large for using on rehabilitation. Our future plan is to reduce the errors by using individual parameters or using multiple formulas determining which one is proper to use automatically.
{"title":"Development of the Wearable Device to Measure a Floor Reacting Force","authors":"Michihiko Fukunaga, Hayato Ichinose, Shogo Ishizaka","doi":"10.1109/BMEICON.2018.8609940","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609940","url":null,"abstract":"The objective of this study is to measure a floor reacting force by the wearable device. We attached six pressure sensors under a sole and created the estimating equation of floor reacting force using the sensor outputs by the data stepping on a force plate. Estimation was tried by the stepping motion and a normal gait. We tried four types of estimating equation: multiple coefficients or a single coefficient and individual or general, respectively. As a result, the errors were minimum during the expecting motion with multiple and individual coefficients. However, the errors increased too much during unexpected motions. In case of such motions, estimation with a single coefficient would rather better. Moreover, using the general estimating equation for any test subjects, estimation with multiple coefficients could not reduce the errors much relative to the case with a single coefficient. These results indicated that using many explanatory variables might be good for a limited condition, however, a few variables might be enough for covering a wide range of situations, motions or test subjects. After all, using a generalized estimating equation, the estimation error was about 20kgf on average, which is too large for using on rehabilitation. Our future plan is to reduce the errors by using individual parameters or using multiple formulas determining which one is proper to use automatically.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128683423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microscope is one of the essential devices in medical profession. It is usually used for finding out types of abnormal cells or tissues, detecting the diseases, and defining a range treatment option for patient. Recently, there is a new technology of whole-slide imaging (WSI) which has been developed in the microscope. The technology provides the scanning of conventional glass slides to produce digital images of cumulative data. Since the WSI is a complex system and very expensive price, hence we aim to implement the prototype of a cost- effective whole – slide imaging system based on the principle of microscopic design. The implemented prototype consisting of optical and mechanical parts was developed using 3D printing. The optical system employed a 40x objective lens aligning with the digital camera for imaging the specimen on the standard slide. The mechanical system was designed for the movement of the slide in 3 dimensions automatically and controlled by stepper motors and microcontrollers based on controlling computer program. To accumulate the whole- slide image, the slide was scanned and captured in the X-Y axes and the focal (Z) axis in a sequence. The cumulative data was then analyzed and rendered the whole- slide image. The implemented device would be advantageous to cytologists and doctors for the biomedical imaging and recording numerous medical data of the patients.
{"title":"Low-cost instrumentation of Automated Whole – slide Microscope for Biomedical Imaging","authors":"Suejit Pechprasarn, Chayanisa Sukkasem, Phitsini Suvarnaphaet, Rujirada Thongchoom, Suteeboon Chuwittaya, M. Janyasupab","doi":"10.1109/BMEICON.2018.8609942","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609942","url":null,"abstract":"Microscope is one of the essential devices in medical profession. It is usually used for finding out types of abnormal cells or tissues, detecting the diseases, and defining a range treatment option for patient. Recently, there is a new technology of whole-slide imaging (WSI) which has been developed in the microscope. The technology provides the scanning of conventional glass slides to produce digital images of cumulative data. Since the WSI is a complex system and very expensive price, hence we aim to implement the prototype of a cost- effective whole – slide imaging system based on the principle of microscopic design. The implemented prototype consisting of optical and mechanical parts was developed using 3D printing. The optical system employed a 40x objective lens aligning with the digital camera for imaging the specimen on the standard slide. The mechanical system was designed for the movement of the slide in 3 dimensions automatically and controlled by stepper motors and microcontrollers based on controlling computer program. To accumulate the whole- slide image, the slide was scanned and captured in the X-Y axes and the focal (Z) axis in a sequence. The cumulative data was then analyzed and rendered the whole- slide image. The implemented device would be advantageous to cytologists and doctors for the biomedical imaging and recording numerous medical data of the patients.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126101793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609946
M. P. Paing, C. Pintavirooj, S. Tungjitkusolmun, S. Choomchuay, K. Hamamoto
Imbalanced data classification is a serious and challenging task for most of the medical image diagnosis applications. They usually produce a larger number of false samples compared to the actual ones. That is the number of samples for the class of interest (minority) is significantly fewer than other types of class (majority). The classification performed using such data is called imbalanced data classification. As a consequence, the learning model bias towards the majority class and fails the classification of the minority class. Data sampling and ensemble methods are common ways to compensate for this issue. Random forest (RF), an ensemble of multiple decision trees, is very famous in both of the classification and regression problems because of its robust and accurate predictions. However, it also suffers class bias in the imbalanced data classification problems. This paper proposes and compares different sampling methods to solve the imbalanced data classification in RF.
{"title":"Comparison of Sampling Methods for Imbalanced Data Classification in Random Forest","authors":"M. P. Paing, C. Pintavirooj, S. Tungjitkusolmun, S. Choomchuay, K. Hamamoto","doi":"10.1109/BMEICON.2018.8609946","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609946","url":null,"abstract":"Imbalanced data classification is a serious and challenging task for most of the medical image diagnosis applications. They usually produce a larger number of false samples compared to the actual ones. That is the number of samples for the class of interest (minority) is significantly fewer than other types of class (majority). The classification performed using such data is called imbalanced data classification. As a consequence, the learning model bias towards the majority class and fails the classification of the minority class. Data sampling and ensemble methods are common ways to compensate for this issue. Random forest (RF), an ensemble of multiple decision trees, is very famous in both of the classification and regression problems because of its robust and accurate predictions. However, it also suffers class bias in the imbalanced data classification problems. This paper proposes and compares different sampling methods to solve the imbalanced data classification in RF.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131287325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609994
C. Janeczek, M. Gföhler, M. Harasek, W. Mohl
An important performance criterion for blood pumps is blood damage due to the non-physiological loads on the blood cells. Especially for miniature pumps that are inserted into blood vessels in catheter devices and – due to their small diameters - operate at high rotational speeds, hemolysis is an important design issue. The Assistocor heart catheter pump is a left ventricular assist device which is inserted into the Aorta and has an outer diameter of 6.8 mm, it operates at rotational speeds up to 40000 rpm. In-vitro tests with animal blood and different pump prototypes of the Assistocor heart pump showed that hemolysis values stayed below the recommended values for five out of nine of the tested prototypes and are comparable with results from literature, indicating that the design process is adequate to keep the damage of blood cells within acceptable limits despite the high rotational speeds.
{"title":"Evaluation of Hemolysis Caused by a Miniature Heart Catheter Pump","authors":"C. Janeczek, M. Gföhler, M. Harasek, W. Mohl","doi":"10.1109/BMEICON.2018.8609994","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609994","url":null,"abstract":"An important performance criterion for blood pumps is blood damage due to the non-physiological loads on the blood cells. Especially for miniature pumps that are inserted into blood vessels in catheter devices and – due to their small diameters - operate at high rotational speeds, hemolysis is an important design issue. The Assistocor heart catheter pump is a left ventricular assist device which is inserted into the Aorta and has an outer diameter of 6.8 mm, it operates at rotational speeds up to 40000 rpm. In-vitro tests with animal blood and different pump prototypes of the Assistocor heart pump showed that hemolysis values stayed below the recommended values for five out of nine of the tested prototypes and are comparable with results from literature, indicating that the design process is adequate to keep the damage of blood cells within acceptable limits despite the high rotational speeds.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114213398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609992
Manao Bunkum, Parkbhum Reanaree, Nutthanan Wanluk, S. Visitsattapongse
One of the most common health risks for bedridden patient is a pressure sore or decubitus ulcers. Pressure sore developed in people who are not able to move or lying in the same position for a long period of time. To solve this problem, a caregiver must change the patient’s position every two hours if possible. In addition, the caregiver already has many kinds of care work to assist the patient such as changing clothes, administering medicine, daily health checking etc. This paper presented a design model of the bed used for bedridden patients, including paralysis, individuals with impaired mobility, and the elderly. The bed consists of an automatic bed lift control system and a patient monitoring system. The bed can change the patient’s position in left or right tilt by lifting the left or right side of the bed up automatically. The system came with a graphic user interface to communicate with the caregiver or physician. The patient monitoring system including, the heart rate monitor, the body temperature monitor and the blood pressure monitor which are then sent to a home or hospital server. All data can then be remotely monitored by caretaker or physician. The proposed of the bed is designed to better assist the daily life of bedridden patients and caregivers.
{"title":"Prototype Modeling of Bed for Bedridden Patients","authors":"Manao Bunkum, Parkbhum Reanaree, Nutthanan Wanluk, S. Visitsattapongse","doi":"10.1109/BMEICON.2018.8609992","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609992","url":null,"abstract":"One of the most common health risks for bedridden patient is a pressure sore or decubitus ulcers. Pressure sore developed in people who are not able to move or lying in the same position for a long period of time. To solve this problem, a caregiver must change the patient’s position every two hours if possible. In addition, the caregiver already has many kinds of care work to assist the patient such as changing clothes, administering medicine, daily health checking etc. This paper presented a design model of the bed used for bedridden patients, including paralysis, individuals with impaired mobility, and the elderly. The bed consists of an automatic bed lift control system and a patient monitoring system. The bed can change the patient’s position in left or right tilt by lifting the left or right side of the bed up automatically. The system came with a graphic user interface to communicate with the caregiver or physician. The patient monitoring system including, the heart rate monitor, the body temperature monitor and the blood pressure monitor which are then sent to a home or hospital server. All data can then be remotely monitored by caretaker or physician. The proposed of the bed is designed to better assist the daily life of bedridden patients and caregivers.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129420660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609960
Yue Wang, Xiaoyu Zheng, Qingyao Liao, Ling Xiao
HIFU is a noninvasive tissue ablation method for treatment of a broad range of cancer and benign maladies. Its ultrasonic energy can also be used to activate or deactivate nerves in recent years. To avoid unnecessary tissue ablation caused by high acoustic energy, treatment time is split. Dual-frequency ultrasound takes advantage of cavitation, but in many cases, increases the complexity of the HIFU system. The objective of this research was to measure the acoustic power of HIFU transducer with split treatment time, and compare it with that of the transducer with traditional treatment time. The effect of frequency difference on acoustic power of dual-frequency HIFU transducer is also evaluated in experiments. The results demonstrate that split treatment time has little effect on acoustic power. As frequency difference increases, the acoustic power does not decrease monotonically. Nevertheless, if frequency difference is above 10kHz, the acoustic power significantly decreases compared with that of smaller frequency differences. For dual-frequency HIFU, appropriate frequency difference should be chosen according to their respective application.
{"title":"The Effect of Treatment Time and Frequency Difference on Acoustic Power of High-intensity Focused Ultrasound","authors":"Yue Wang, Xiaoyu Zheng, Qingyao Liao, Ling Xiao","doi":"10.1109/BMEICON.2018.8609960","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609960","url":null,"abstract":"HIFU is a noninvasive tissue ablation method for treatment of a broad range of cancer and benign maladies. Its ultrasonic energy can also be used to activate or deactivate nerves in recent years. To avoid unnecessary tissue ablation caused by high acoustic energy, treatment time is split. Dual-frequency ultrasound takes advantage of cavitation, but in many cases, increases the complexity of the HIFU system. The objective of this research was to measure the acoustic power of HIFU transducer with split treatment time, and compare it with that of the transducer with traditional treatment time. The effect of frequency difference on acoustic power of dual-frequency HIFU transducer is also evaluated in experiments. The results demonstrate that split treatment time has little effect on acoustic power. As frequency difference increases, the acoustic power does not decrease monotonically. Nevertheless, if frequency difference is above 10kHz, the acoustic power significantly decreases compared with that of smaller frequency differences. For dual-frequency HIFU, appropriate frequency difference should be chosen according to their respective application.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123674055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609928
Jeerawan Thanarak, Hauwa Mohammed, Samand Pashneh-Tala, F. Claeyssens, N. Green
Poly(glycerol sebacate)-methacrylate (PGS-M) is a photocurable form of polyglycerol sebacate (PGS) that has recently been shown to be suitable for use as a scaffold for tissue engineering. It has the benefits of PGS, including biocompatibility and biodegradability, while also being much simpler to process into a variety of 3D structures. Cell compatibility has already been demonstrated on the 30% methacrylated PGS-M scaffolds. However no studies have yet assessed the collagen produced by cells growing on the PGS-M scaffold. Here we demonstrate that 50% methacrylated PGS-M 3D scaffolds are able to support the culture of human dermal fibroblasts for 1 week. We also show that collagen production is enhanced compared with the same cells growing on tissue culture plastic, with the cells producing approximately 50% more total collagen after 1 week in culture. These results go further to demonstrate the suitability of the PGS-M scaffolds for generating ECM based constructs for soft tissue engineering.
{"title":"Enhanced Collagen Production from Human Dermal Fibroblasts on Poly(glycerol sebacate)-methacrylate Scaffolds","authors":"Jeerawan Thanarak, Hauwa Mohammed, Samand Pashneh-Tala, F. Claeyssens, N. Green","doi":"10.1109/BMEICON.2018.8609928","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609928","url":null,"abstract":"Poly(glycerol sebacate)-methacrylate (PGS-M) is a photocurable form of polyglycerol sebacate (PGS) that has recently been shown to be suitable for use as a scaffold for tissue engineering. It has the benefits of PGS, including biocompatibility and biodegradability, while also being much simpler to process into a variety of 3D structures. Cell compatibility has already been demonstrated on the 30% methacrylated PGS-M scaffolds. However no studies have yet assessed the collagen produced by cells growing on the PGS-M scaffold. Here we demonstrate that 50% methacrylated PGS-M 3D scaffolds are able to support the culture of human dermal fibroblasts for 1 week. We also show that collagen production is enhanced compared with the same cells growing on tissue culture plastic, with the cells producing approximately 50% more total collagen after 1 week in culture. These results go further to demonstrate the suitability of the PGS-M scaffolds for generating ECM based constructs for soft tissue engineering.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127441710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.1109/BMEICON.2018.8609931
Yoshiki Tojyo, Haruna Saito, Keigo Enomoto, H. Kudo
A microfluidic biosensor for real-time monitoring of lactic acid (LA) contained in sweat was fabricated and tested. Sweat is one of the most promising body fluids for non-invasive assessment of biological components reflecting physiological condition. In order to measure sweat LA continuously, we developed a microfluidic biosensor, which is highly specific with LA, and a sampling element that transports whole secretions to the biosensor. In this study, the biosensor was first fabricated and its fundamental characteristics were investigated. The biosensor showed sufficient sensitivity (0.16 nA μM−1) to measure sweat LA and high specific activity suitable for the sweat monitoring purpose was also confirmed. Real-sample test was also carried out using our system. The microfluidic biosensor was attached on the upper arm of healthy male subject. Sweat LA was continuously monitored during bike exercise. As a result, elevation of LA was successfully detected (62.4 μg/cm2/min for anaerobic exercise), and the signal decreased to the background level after the exercise. This possibility of non-invasive evaluation of metabolic activity was demonstrated.
制备了一种用于实时监测汗液中乳酸的微流控生物传感器，并对其进行了测试。汗液是反映生理状况的生物成分的无创评估最有前途的体液之一。为了连续测量汗液的LA，我们开发了一种微流体生物传感器，它具有高度的LA特异性，以及一种将整个分泌物输送到生物传感器的采样元件。本研究首先制备了生物传感器，并对其基本特性进行了研究。该生物传感器具有足够的灵敏度(0.16 nA μM−1)来测量汗液LA，并且具有适合汗液监测目的的高比活性。并利用该系统进行了实样本测试。将微流控生物传感器贴于健康男性受试者上臂。在骑自行车运动期间连续监测汗液LA。结果，成功检测到LA升高(无氧运动时为62.4 μg/cm2/min)，运动后信号降至背景水平。这种无创评估代谢活动的可能性得到了证实。
{"title":"Sweat Lactic Acid Biosensor using Continuous Flow as Sample Career","authors":"Yoshiki Tojyo, Haruna Saito, Keigo Enomoto, H. Kudo","doi":"10.1109/BMEICON.2018.8609931","DOIUrl":"https://doi.org/10.1109/BMEICON.2018.8609931","url":null,"abstract":"A microfluidic biosensor for real-time monitoring of lactic acid (LA) contained in sweat was fabricated and tested. Sweat is one of the most promising body fluids for non-invasive assessment of biological components reflecting physiological condition. In order to measure sweat LA continuously, we developed a microfluidic biosensor, which is highly specific with LA, and a sampling element that transports whole secretions to the biosensor. In this study, the biosensor was first fabricated and its fundamental characteristics were investigated. The biosensor showed sufficient sensitivity (0.16 nA μM−1) to measure sweat LA and high specific activity suitable for the sweat monitoring purpose was also confirmed. Real-sample test was also carried out using our system. The microfluidic biosensor was attached on the upper arm of healthy male subject. Sweat LA was continuously monitored during bike exercise. As a result, elevation of LA was successfully detected (62.4 μg/cm2/min for anaerobic exercise), and the signal decreased to the background level after the exercise. This possibility of non-invasive evaluation of metabolic activity was demonstrated.","PeriodicalId":232271,"journal":{"name":"2018 11th Biomedical Engineering International Conference (BMEiCON)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127517724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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