Abstract Objectives Cancer chemotherapeutic treatments come with many adverse effects. Anticancer studies with natural products have been carried out to minimize these issues. This study aimed to evaluate the anticancer potential of endemic Phlomis brevibracteata Turrill against four different Hepatocellular Carcinoma (HCC) cell lines and find new natural candidates for cancer treatment. Methods The chemical composition of 70 % aqueous methanol extract (PBM) of P. brevibracteata was analyzed using the LC-MS/MS method. Additionally, the effect of PBM on the proliferation, motility, and oxidative state of four different HCC cell lines of SK-HEP-1, PLC/PRF/5, HuH-7, and Mahlavu have been investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), wound healing, and DCFH-DA assays respectively. Results Our results identified caffeoylquinic acids and Forsythoside B as the main chemical constituents of the PBM. A significant decrease in cell viability was recorded at certain extract concentrations. The motility of the HCC cell lines was inhibited at different levels when treated with PBM. PBM reduced basal and induced oxidative states in a concentration-dependent manner. Conclusions We conclude P. brevibracteata plant extract can be a potential candidate for further studies with the goal of new anticancer chemotherapeutic discovery.
{"title":"New data for endemic Phlomis brevibracteata Turrill from North Cyprus: biological activities and chemical composition","authors":"I. Kunter, Niloufar Zabib, Fatih Göger, M. Koşar","doi":"10.1515/tjb-2023-0012","DOIUrl":"https://doi.org/10.1515/tjb-2023-0012","url":null,"abstract":"Abstract Objectives Cancer chemotherapeutic treatments come with many adverse effects. Anticancer studies with natural products have been carried out to minimize these issues. This study aimed to evaluate the anticancer potential of endemic Phlomis brevibracteata Turrill against four different Hepatocellular Carcinoma (HCC) cell lines and find new natural candidates for cancer treatment. Methods The chemical composition of 70 % aqueous methanol extract (PBM) of P. brevibracteata was analyzed using the LC-MS/MS method. Additionally, the effect of PBM on the proliferation, motility, and oxidative state of four different HCC cell lines of SK-HEP-1, PLC/PRF/5, HuH-7, and Mahlavu have been investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), wound healing, and DCFH-DA assays respectively. Results Our results identified caffeoylquinic acids and Forsythoside B as the main chemical constituents of the PBM. A significant decrease in cell viability was recorded at certain extract concentrations. The motility of the HCC cell lines was inhibited at different levels when treated with PBM. PBM reduced basal and induced oxidative states in a concentration-dependent manner. Conclusions We conclude P. brevibracteata plant extract can be a potential candidate for further studies with the goal of new anticancer chemotherapeutic discovery.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76646335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Oxidative stress is closely associated with atherosclerosis and acute coronary syndromes. The purpose of this study was to evaluate well-known and proportional oxidative stress biomarkers in ST-Elevation Myocardial Infarction (STEMI) patients. Methods In this single center, prospective and cross-sectional study, 107 individuals (63 patients) were studied. Total oxidative status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), ischemia modified albumin (IMA), myeloperoxidase (MPO), paraoxonase-1 (PON-1) and arylesterase (AREase) enzyme activities as well as MPO/PON-1, MPO/AREase and MPO/HDL-C ratios were studied. As short-term in-hospital prognosis biomarkers; in-hospital mortality, early systolic dysfunction and spontaneous complete revascularization were investigated. Results Our results indicated that TOS, OSI, IMA, MPO, MPO/PON-1 and MPO/HDL ratios were significantly higher, PON-1 and AREase were significantly lower in STEMI patients compared to the control group. However, in the regression analysis performed by adjusting the differences between the groups, only IMA was found as an independent risk factor (OR=2.711, 95 % CI=1.094–6.719, p=0.031). In terms of in-hospital short-term prognostic biomarkers, a significant relationship was found only between OSI and spontaneous complete revascularization. The OSI value was higher in the group with TIMI grade 3 flow than in the group with TIMI grade 0–2 flow (2.42 [0.81–4.49] vs. 1.63 [0.33–6.07], p=0.016). Conclusions In STEMI patients, both the well-known (TOS, OSI, and MPO) and proportional (MPO/PON-1 and MPO/HDL cholesterol ratios) oxidative stress markers were elevated and can be considered as having a role in the pathogenesis of STEMI.
{"title":"Evaluation of oxidative stress biomarkers together with myeloperoxidase/paraoxonase-1 and myeloperoxidase/high density lipoprotein cholesterol in ST-elevation myocardial infarction","authors":"A. Hamamcioglu, Belma Kalaycı, S. Kalaycı","doi":"10.1515/tjb-2022-0200","DOIUrl":"https://doi.org/10.1515/tjb-2022-0200","url":null,"abstract":"Abstract Objectives Oxidative stress is closely associated with atherosclerosis and acute coronary syndromes. The purpose of this study was to evaluate well-known and proportional oxidative stress biomarkers in ST-Elevation Myocardial Infarction (STEMI) patients. Methods In this single center, prospective and cross-sectional study, 107 individuals (63 patients) were studied. Total oxidative status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), ischemia modified albumin (IMA), myeloperoxidase (MPO), paraoxonase-1 (PON-1) and arylesterase (AREase) enzyme activities as well as MPO/PON-1, MPO/AREase and MPO/HDL-C ratios were studied. As short-term in-hospital prognosis biomarkers; in-hospital mortality, early systolic dysfunction and spontaneous complete revascularization were investigated. Results Our results indicated that TOS, OSI, IMA, MPO, MPO/PON-1 and MPO/HDL ratios were significantly higher, PON-1 and AREase were significantly lower in STEMI patients compared to the control group. However, in the regression analysis performed by adjusting the differences between the groups, only IMA was found as an independent risk factor (OR=2.711, 95 % CI=1.094–6.719, p=0.031). In terms of in-hospital short-term prognostic biomarkers, a significant relationship was found only between OSI and spontaneous complete revascularization. The OSI value was higher in the group with TIMI grade 3 flow than in the group with TIMI grade 0–2 flow (2.42 [0.81–4.49] vs. 1.63 [0.33–6.07], p=0.016). Conclusions In STEMI patients, both the well-known (TOS, OSI, and MPO) and proportional (MPO/PON-1 and MPO/HDL cholesterol ratios) oxidative stress markers were elevated and can be considered as having a role in the pathogenesis of STEMI.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81655694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Centrifugation is a time-consuming step which increases the turnaround time (TAT) in laboratories. A few studies have addressed the effect of altering centrifugation settings on analytical quality for clinical chemistry analytes, and most of these studies have used collection tubes with gel separators. However, gel separator tubes may be unsuitable for some laboratories because they are slightly more expensive than tubes without gel separators and are not appropriate for some special tests. The aim of this study was to investigate the effect of centrifugation conditions on clinical chemistry analytes. Methods We compared centrifugation times of 7 min at 2,200×g and 5 min at 2,750×g with the manufacturer’s protocol of 10 min at 1,300×g as the reference condition. Twenty general chemistry analytes were studied in lithium heparin plasma tubes without gel separators. Results For all analytes except carbon dioxide (CO2), no significant differences in analyte results were observed when the centrifugation time was reduced. Deming regression and Bland–Altman plots demonstrated an acceptable clinical concordance within the limits of total allowable error for all analytes between the two rapid centrifugation conditions with the reference centrifugation condition. Conclusions Our results confirmed that alternate centrifugation conditions for either 7 min at 2,200×g or 5 min at 2,750×g of samples collected in lithium heparin tubes without gel are acceptable for clinical chemistry analytes. Our data support using centrifugation at higher speeds for shorter times to improve TAT without altering the quality of the analytical results.
{"title":"Influence of reduced centrifugation time on clinical chemistry analytes and literature review","authors":"Piraya Tantisaranon, Kanyarat Dumkengkhachornwong, Areerat Hnoonual","doi":"10.1515/tjb-2022-0211","DOIUrl":"https://doi.org/10.1515/tjb-2022-0211","url":null,"abstract":"Abstract Objectives Centrifugation is a time-consuming step which increases the turnaround time (TAT) in laboratories. A few studies have addressed the effect of altering centrifugation settings on analytical quality for clinical chemistry analytes, and most of these studies have used collection tubes with gel separators. However, gel separator tubes may be unsuitable for some laboratories because they are slightly more expensive than tubes without gel separators and are not appropriate for some special tests. The aim of this study was to investigate the effect of centrifugation conditions on clinical chemistry analytes. Methods We compared centrifugation times of 7 min at 2,200×g and 5 min at 2,750×g with the manufacturer’s protocol of 10 min at 1,300×g as the reference condition. Twenty general chemistry analytes were studied in lithium heparin plasma tubes without gel separators. Results For all analytes except carbon dioxide (CO2), no significant differences in analyte results were observed when the centrifugation time was reduced. Deming regression and Bland–Altman plots demonstrated an acceptable clinical concordance within the limits of total allowable error for all analytes between the two rapid centrifugation conditions with the reference centrifugation condition. Conclusions Our results confirmed that alternate centrifugation conditions for either 7 min at 2,200×g or 5 min at 2,750×g of samples collected in lithium heparin tubes without gel are acceptable for clinical chemistry analytes. Our data support using centrifugation at higher speeds for shorter times to improve TAT without altering the quality of the analytical results.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"72 1","pages":"376 - 387"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76035393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Murat Kaya, Ilknur Suer, E. Ozgur, Ozel Capik, O. Karatas, S. Ozturk, U. Gezer, S. Palanduz, K. Çefle
Abstract Objectives Multiple myeloma (MM) is a common hematological cancer. Hence, it is important to conduct further studies investigating the molecular mechanisms in detail that contributes to myeloma genesis. In addition to genetic changes, epigenetic factors such as miRNAs may influence the expression of myeloma-related genes. Methods Our study aimed to detect genes closely related to MM and miRNAs involved in the cancer process by changing the expression of these genes with bioinformatics tools and in vitro methods. Bioinformatics approaches identified hub miRNAs in our study that may have a role in the expression change of genes connected to myeloma. The functional impacts of the chosen miRNA on RPMI8226 and U266 cell lines and the effect of this miRNA on the expression changes of putative target genes were investigated. Results The viability of miR-145-5p transfected cells was found to decrease compared to control cells and the expression of IGF1R and NRAS genes were found to be significantly suppressed in both cell lines at mRNA level. Decreased levels of the IGF1R and NRAS genes were confirmed in miR-145-5p transfected cells at the protein level as well as compared to control cells. In addition, IGF1R/miR-145-5p interaction was demonstrated via luciferase reporter assay. However, expression levels of EGFR, KLF4, IRS1, CDK4 and CDK6 candidate genes had no statistically significant difference in miR-145-5p transfected cells compared to control cells. Conclusions Mir-145-5p was demonstrated to act as a tumor suppressor miRNA and inhibit the proliferation in MM cell lines via targeting IGF1R and NRAS.
{"title":"miR-145-5p suppresses cell proliferation by targeting IGF1R and NRAS genes in multiple myeloma cells","authors":"Murat Kaya, Ilknur Suer, E. Ozgur, Ozel Capik, O. Karatas, S. Ozturk, U. Gezer, S. Palanduz, K. Çefle","doi":"10.1515/tjb-2023-0042","DOIUrl":"https://doi.org/10.1515/tjb-2023-0042","url":null,"abstract":"Abstract Objectives Multiple myeloma (MM) is a common hematological cancer. Hence, it is important to conduct further studies investigating the molecular mechanisms in detail that contributes to myeloma genesis. In addition to genetic changes, epigenetic factors such as miRNAs may influence the expression of myeloma-related genes. Methods Our study aimed to detect genes closely related to MM and miRNAs involved in the cancer process by changing the expression of these genes with bioinformatics tools and in vitro methods. Bioinformatics approaches identified hub miRNAs in our study that may have a role in the expression change of genes connected to myeloma. The functional impacts of the chosen miRNA on RPMI8226 and U266 cell lines and the effect of this miRNA on the expression changes of putative target genes were investigated. Results The viability of miR-145-5p transfected cells was found to decrease compared to control cells and the expression of IGF1R and NRAS genes were found to be significantly suppressed in both cell lines at mRNA level. Decreased levels of the IGF1R and NRAS genes were confirmed in miR-145-5p transfected cells at the protein level as well as compared to control cells. In addition, IGF1R/miR-145-5p interaction was demonstrated via luciferase reporter assay. However, expression levels of EGFR, KLF4, IRS1, CDK4 and CDK6 candidate genes had no statistically significant difference in miR-145-5p transfected cells compared to control cells. Conclusions Mir-145-5p was demonstrated to act as a tumor suppressor miRNA and inhibit the proliferation in MM cell lines via targeting IGF1R and NRAS.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78138650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mustafa Karademir, H. Doğan, Z. D. Sahin İnan, K. Dogan, Demet Kablan
Abstract Objectives Our study aimed to investigate the role of endoplasmic reticulum stress (ER) in brain damage following hepatic ischemia-reperfusion (HIR) injury. Specifically, we characterized the expression of markers of ER stress and histopathologic changes in the brain following HIR. Methods 12 adults female Wistar rats were divided into two experimental groups equally. Group 1 was designed as the control group, and Group 2 was designed as the HIR group. Blood, liver, and brain tissue samples were collected during the sacrifice. The quantitative ELISA kits were used to detect glucose-regulated protein 78 (GRP-78), activating transcription factor 4 (ATF-4), eukaryotic initiation factor 2 alpha (EIF2-A), caspase-3, caspase-9, and CCAAT/enhancer-binding protein (CEBP) in plasma. Histopathological examination was performed for liver and brain tissues. Results Higher levels of GRP-78 (p=0.006), ATF4 (p=0.001), and EIF2-Α (p=0.007) were detected in group 2. More damage was detected in liver and brain samples in the histopathological examination of group 2 than in group 1. Conclusions Our results demonstrate that ER stress is involved in developing brain damage following hepatic ischemia-reperfusion injury, as evidenced by increased expression of markers of ER stress and neuronal injury.
{"title":"Increased endoplasmic reticulum stress might be related to brain damage in hepatic ischemia-reperfusion injury","authors":"Mustafa Karademir, H. Doğan, Z. D. Sahin İnan, K. Dogan, Demet Kablan","doi":"10.1515/tjb-2022-0292","DOIUrl":"https://doi.org/10.1515/tjb-2022-0292","url":null,"abstract":"Abstract Objectives Our study aimed to investigate the role of endoplasmic reticulum stress (ER) in brain damage following hepatic ischemia-reperfusion (HIR) injury. Specifically, we characterized the expression of markers of ER stress and histopathologic changes in the brain following HIR. Methods 12 adults female Wistar rats were divided into two experimental groups equally. Group 1 was designed as the control group, and Group 2 was designed as the HIR group. Blood, liver, and brain tissue samples were collected during the sacrifice. The quantitative ELISA kits were used to detect glucose-regulated protein 78 (GRP-78), activating transcription factor 4 (ATF-4), eukaryotic initiation factor 2 alpha (EIF2-A), caspase-3, caspase-9, and CCAAT/enhancer-binding protein (CEBP) in plasma. Histopathological examination was performed for liver and brain tissues. Results Higher levels of GRP-78 (p=0.006), ATF4 (p=0.001), and EIF2-Α (p=0.007) were detected in group 2. More damage was detected in liver and brain samples in the histopathological examination of group 2 than in group 1. Conclusions Our results demonstrate that ER stress is involved in developing brain damage following hepatic ischemia-reperfusion injury, as evidenced by increased expression of markers of ER stress and neuronal injury.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86112430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serdar Doğan, H. Okuyan, Tayibe Bal, M. Çabalak, Mehmet A. Begen
Abstract Objectives Roles of Thrombospondin-1 (TSP-1) and Thrombospondin-2 (TSP-2) in tissue repair and inflammation are well-documented, but the association of their serum expressions with the pathogenesis of COVID-19 remains unclear. We investigate the roles of TSP-1 and TSP-2 in COVID-19. Methods 106 SARS-CoV-2 infected patients and 23 healthy people were enrolled in our study. COVID-19 patients were divided into two groups as non-severe and severe. TSP-1 and TSP-2 concentrations were measured with an enzyme-linked Immunosorbent Assay, and blood markers were analyzed with routine laboratory techniques. Results COVID-19 patients had significantly higher TSP-1 and TSP-2 levels than healthy controls. TSP-1 and TSP-2 positively correlated with inflammatory markers, including ESR, CRP, PCT, ferritin, and biochemical parameters such as ALT, AST, BUN, CK, and LDH. In addition, TSP-1 and TSP-2 were negatively correlated with hematological markers such as LYM, EOS, and HGB. Receiver operating characteristic analyses revealed that COVID-19 may be predicted with TSP-1 levels over 189.94 ng/mL and TSP-2 levels higher than 0.70 ng/mL. Conclusions Our analysis suggests that TSP-1 and TSP-2 expressions at the systemic level may have clinical importance for COVID-19.
{"title":"Relationship of Thrombospondin-1 and Thrombospondin-2 with hematological, biochemical and inflammatory markers in COVID-19 patients","authors":"Serdar Doğan, H. Okuyan, Tayibe Bal, M. Çabalak, Mehmet A. Begen","doi":"10.1515/tjb-2022-0265","DOIUrl":"https://doi.org/10.1515/tjb-2022-0265","url":null,"abstract":"Abstract Objectives Roles of Thrombospondin-1 (TSP-1) and Thrombospondin-2 (TSP-2) in tissue repair and inflammation are well-documented, but the association of their serum expressions with the pathogenesis of COVID-19 remains unclear. We investigate the roles of TSP-1 and TSP-2 in COVID-19. Methods 106 SARS-CoV-2 infected patients and 23 healthy people were enrolled in our study. COVID-19 patients were divided into two groups as non-severe and severe. TSP-1 and TSP-2 concentrations were measured with an enzyme-linked Immunosorbent Assay, and blood markers were analyzed with routine laboratory techniques. Results COVID-19 patients had significantly higher TSP-1 and TSP-2 levels than healthy controls. TSP-1 and TSP-2 positively correlated with inflammatory markers, including ESR, CRP, PCT, ferritin, and biochemical parameters such as ALT, AST, BUN, CK, and LDH. In addition, TSP-1 and TSP-2 were negatively correlated with hematological markers such as LYM, EOS, and HGB. Receiver operating characteristic analyses revealed that COVID-19 may be predicted with TSP-1 levels over 189.94 ng/mL and TSP-2 levels higher than 0.70 ng/mL. Conclusions Our analysis suggests that TSP-1 and TSP-2 expressions at the systemic level may have clinical importance for COVID-19.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82980654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmet Dumanlı, E. Günay, Suphi Aydın, Ş. Çilekar, A. Gencer, Emira Kurbaseviç, Gürhan Öz, Sefa Çelik, A. Balcı, Mehmet Özcan, Mujgan Ercan Karadag
Abstract Objectives We aimed to investigate the usability of pleural pyruvate kinase (PK), total antioxidant status (TAS), and total oxidant status (TOS) as an alternative to Light’s criteria in exudate-transudate differentiation. Methods This prospective study was conducted among 84 patients (42 transudates and 42 exudates) with pleural effusion. The levels of PK, TAS, and TOS were measured by using ELISA kits, and the ROC analysis was used to evaluate the diagnostic efficiency. Results PK (p=0.001), TAS (p=0.027), and TOS (p=0.002) levels in pleural fluids were found to be significantly higher in the exudate group. The cut-off values for PK, TAS, and TOS were 10.64 U/L, 13.54 mmol trolox equivalent/L, and 13.88 μmol H2O2 equivalent/L, respectively. While the sensitivity values were 97.62 % for PK, 66.67 % for TAS, and 64.29 % for TOS, the specificity values were 80.95 % for PK, 52.38 % for TAS, and 57.14 % for TOS. Conclusions PK levels in pleural effusion can be useful in suspected cases to differentiate between exudate and transudate in addition to Light’s criteria. However, pleural TOS and TAS parameters could not be as sensitive and specific as Light’s criteria.
{"title":"Evaluation of pyruvate kinase and oxidative stress parameters in differentiation between transudate and exudate in pleural liquids","authors":"Ahmet Dumanlı, E. Günay, Suphi Aydın, Ş. Çilekar, A. Gencer, Emira Kurbaseviç, Gürhan Öz, Sefa Çelik, A. Balcı, Mehmet Özcan, Mujgan Ercan Karadag","doi":"10.1515/tjb-2022-0255","DOIUrl":"https://doi.org/10.1515/tjb-2022-0255","url":null,"abstract":"Abstract Objectives We aimed to investigate the usability of pleural pyruvate kinase (PK), total antioxidant status (TAS), and total oxidant status (TOS) as an alternative to Light’s criteria in exudate-transudate differentiation. Methods This prospective study was conducted among 84 patients (42 transudates and 42 exudates) with pleural effusion. The levels of PK, TAS, and TOS were measured by using ELISA kits, and the ROC analysis was used to evaluate the diagnostic efficiency. Results PK (p=0.001), TAS (p=0.027), and TOS (p=0.002) levels in pleural fluids were found to be significantly higher in the exudate group. The cut-off values for PK, TAS, and TOS were 10.64 U/L, 13.54 mmol trolox equivalent/L, and 13.88 μmol H2O2 equivalent/L, respectively. While the sensitivity values were 97.62 % for PK, 66.67 % for TAS, and 64.29 % for TOS, the specificity values were 80.95 % for PK, 52.38 % for TAS, and 57.14 % for TOS. Conclusions PK levels in pleural effusion can be useful in suspected cases to differentiate between exudate and transudate in addition to Light’s criteria. However, pleural TOS and TAS parameters could not be as sensitive and specific as Light’s criteria.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81476896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Periodontal diseases are chronic diseases of oral cavity comprising of inflammatory conditions which effect the supporting structures of dentition. It is a multifactorial disease which is also known to be affected by genetic and environmental factors. However, some of the clinical parameters such as probing depth, attachment level, plaque index, bleeding on probing and radiographic assessment of alveolar bone are known to assess the severity of disease, although the disease activity is not measured. In the current scenario the salivary diagnostic markers for diagnosis of periodontal diseases have included the salivary enzymes, immunoglobulins, bacterial components or products, phenotypic markers such as epithelial markers. Also, saliva is a mirror of oral and systemic health and a valuable source to find out the physiological aspects of periodontal diseases. The present review thus highlights various salivary biomarkers which are quick, easy and reliable method for assessing and monitoring periodontal disease that improves and speeds treatment decisions and moves the field closer to individualized point-of-care diagnostics.
{"title":"Predictive salivary biomarkers for early diagnosis of periodontal diseases – current and future developments","authors":"Fang-ping Shi, Wei Liu, Yuexian Yao, Qingbin Zhang, Zhengji Chen, Yankui Xian, Bhavana Sujanamulk","doi":"10.1515/tjb-2022-0153","DOIUrl":"https://doi.org/10.1515/tjb-2022-0153","url":null,"abstract":"Abstract Periodontal diseases are chronic diseases of oral cavity comprising of inflammatory conditions which effect the supporting structures of dentition. It is a multifactorial disease which is also known to be affected by genetic and environmental factors. However, some of the clinical parameters such as probing depth, attachment level, plaque index, bleeding on probing and radiographic assessment of alveolar bone are known to assess the severity of disease, although the disease activity is not measured. In the current scenario the salivary diagnostic markers for diagnosis of periodontal diseases have included the salivary enzymes, immunoglobulins, bacterial components or products, phenotypic markers such as epithelial markers. Also, saliva is a mirror of oral and systemic health and a valuable source to find out the physiological aspects of periodontal diseases. The present review thus highlights various salivary biomarkers which are quick, easy and reliable method for assessing and monitoring periodontal disease that improves and speeds treatment decisions and moves the field closer to individualized point-of-care diagnostics.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76279175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Özlem Şahin Ata, Cenk Fatih Canakci, Yerda Özkan Karasu
Abstract Objectives This study aimed to examine the effects of chronic periodontitis and essential hypertension on serum and salivary cartonectin (CTRP3) and procalcitonin (ProCT) levels. Methods 60 non-smokers were seperated into four groups as; 15 people with essential hypertension (EH) and chronic periodontitis (CP) (HT+ CP+), 15 with EH (HT+ CP-), 15 with CP (HT- CP+), 15 control (HT- CP-). PPD, CAL, PI and GI were measured. All groups had their serum and saliva samples collected. Serum and saliva procalcitonin (ProCT) were measured using an electroluminescence method, and cartonectin (CTRP3) levels were determined by enzyme-linked immunosorbent assay. Results When compared to the control group, serum and saliva cartonectin (CTRP3) levels were considerably lower in all groups (respectively p<0.0001, p<0.0001). The serum cartonectin (CTRP3) levels were substantially higher in the HT- CP+ group than in the HT+ CP- group (p=0.002). Serum procalcitonin (ProCT) concentrations were found to be lowest in the HT- CP- group and highest in the HT+ CP+ group. Serum ProCT concentrations did not vary significantly across groups (p=0.110). Salivary procalcitonin (ProCT) levels were below the detection limit in all groups. Conclusions When periodontitis coexist with hypertension in individuals, they may have adversely affect each other due to the same sathways in the pathogenesis of these two disorders. So we can suggest that, serum and saliva cartonectin (CTRP3) may play a role during hypertension and periodontal inflammation and represent a novel future therapeutic target.
{"title":"The levels of cartonectin and procalcitonin in patients with chronic periodontitis and hypertension","authors":"Özlem Şahin Ata, Cenk Fatih Canakci, Yerda Özkan Karasu","doi":"10.1515/tjb-2022-0237","DOIUrl":"https://doi.org/10.1515/tjb-2022-0237","url":null,"abstract":"Abstract Objectives This study aimed to examine the effects of chronic periodontitis and essential hypertension on serum and salivary cartonectin (CTRP3) and procalcitonin (ProCT) levels. Methods 60 non-smokers were seperated into four groups as; 15 people with essential hypertension (EH) and chronic periodontitis (CP) (HT+ CP+), 15 with EH (HT+ CP-), 15 with CP (HT- CP+), 15 control (HT- CP-). PPD, CAL, PI and GI were measured. All groups had their serum and saliva samples collected. Serum and saliva procalcitonin (ProCT) were measured using an electroluminescence method, and cartonectin (CTRP3) levels were determined by enzyme-linked immunosorbent assay. Results When compared to the control group, serum and saliva cartonectin (CTRP3) levels were considerably lower in all groups (respectively p<0.0001, p<0.0001). The serum cartonectin (CTRP3) levels were substantially higher in the HT- CP+ group than in the HT+ CP- group (p=0.002). Serum procalcitonin (ProCT) concentrations were found to be lowest in the HT- CP- group and highest in the HT+ CP+ group. Serum ProCT concentrations did not vary significantly across groups (p=0.110). Salivary procalcitonin (ProCT) levels were below the detection limit in all groups. Conclusions When periodontitis coexist with hypertension in individuals, they may have adversely affect each other due to the same sathways in the pathogenesis of these two disorders. So we can suggest that, serum and saliva cartonectin (CTRP3) may play a role during hypertension and periodontal inflammation and represent a novel future therapeutic target.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86894528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Bildirici, Sedat Gülten, Neslihan Cihan Çalışgan
Abstract Objectives The reference interval is the primary tool used to interpret laboratory test results. Each laboratory should determine reference intervals (RIs) that reflect their population. In this study, it was aimed to determine the RIs of hemogram routine and advanced clinical test parameters for our hospital and region by indirect method and to compare these calculated RIs with the limits recommended by the current manufacturer and the literature. Methods The hemogram results of patients aged 18–65 years who applied to Kastamonu Training and Research Hospital between July 2020 and June 2022, were included in the study. Hemogram analyzes were performed on Sysmex XN-1000 (Kobe, Japan) hematology auto analyzers. The RIs were determined by indirect method from the obtained data using the non-parametric percentage estimation method. Harris-Boyd method was used to decide on subgroup separation based on gender. Results All parameters had non-parametric distribution. RBC, HGB, HCT, MCH, MCHC, PLT, RDW-CV, RDW-SD, PCT, Monocytes count, Eosinophils count, Monocytes % and MacroR parameters which required gender-spesific RIs were determined separately for genders. Conclusions When the results are evaluated, it shows that the manufacturer’s recommendations together with the studies in the literature do not fully reflect the RIs of our population. Therefore, it is very important for each laboratory to determine its own RIs due to the differences in population, diet, technical equipment used and reference group. In addition, we think that our study will make a significant contribution to the literature, since there is insufficient data in the literature on RIs for advanced clinical parameters.
{"title":"Determination of reference intervals of hemogram with advanced clinical parameters by indirect method on Sysmex XN-1000","authors":"M. A. Bildirici, Sedat Gülten, Neslihan Cihan Çalışgan","doi":"10.1515/tjb-2022-0287","DOIUrl":"https://doi.org/10.1515/tjb-2022-0287","url":null,"abstract":"Abstract Objectives The reference interval is the primary tool used to interpret laboratory test results. Each laboratory should determine reference intervals (RIs) that reflect their population. In this study, it was aimed to determine the RIs of hemogram routine and advanced clinical test parameters for our hospital and region by indirect method and to compare these calculated RIs with the limits recommended by the current manufacturer and the literature. Methods The hemogram results of patients aged 18–65 years who applied to Kastamonu Training and Research Hospital between July 2020 and June 2022, were included in the study. Hemogram analyzes were performed on Sysmex XN-1000 (Kobe, Japan) hematology auto analyzers. The RIs were determined by indirect method from the obtained data using the non-parametric percentage estimation method. Harris-Boyd method was used to decide on subgroup separation based on gender. Results All parameters had non-parametric distribution. RBC, HGB, HCT, MCH, MCHC, PLT, RDW-CV, RDW-SD, PCT, Monocytes count, Eosinophils count, Monocytes % and MacroR parameters which required gender-spesific RIs were determined separately for genders. Conclusions When the results are evaluated, it shows that the manufacturer’s recommendations together with the studies in the literature do not fully reflect the RIs of our population. Therefore, it is very important for each laboratory to determine its own RIs due to the differences in population, diet, technical equipment used and reference group. In addition, we think that our study will make a significant contribution to the literature, since there is insufficient data in the literature on RIs for advanced clinical parameters.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82555576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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