Ling He, Jie Zhang, Yang Peng, Hongwei Wu, Zhiqiang Sun
Abstract Objectives Long noncoding RNAs (lncRNAs) plays important role in disease spread and its invasion. Overexpression of prostate cancer antigen 3 gene (PCA3gene) is reported in prostate cancer. To analyze the PCA3 lncRNA expression in chronic myeloid leukemia (CML) patients. Methods The study included clinically confirmed 100 CML patients and 100 healthy subjects. Relative quantification using Sybr Green dye was used to calculate the PCA3 lncRNAs expression. Total RNA was extracted by TRIzol method and quantitative real-time polymerase chain reaction. Results In CML patients, 9.96 ± 4.77-folds increased noncoding PCA3 lncRNA expression was observed compared to healthy subjects. Patients of chronic phase, accelerated phase, and blast crisis phase had 4.46 ± 1.36, 7.31 ± 3.10, and 12.91 ± 4.85-fold PCA3 lncRNA expression compared to healthy subjects (p<0.0001), respectively. CML patients who have a complaint of splenomegaly had higher PCA3 lncRNA expression than those who did not complain splenomegaly compared to healthy subjects (12.04 ± 5.02-fold vs. 6.09 ± 3.39-fold, p<0.0001). Patients who had ≤20,000 TLC showed fewer PCA3 lncRNA expression than those who had >20 thousand of TLC (4.45 ± 1.84 vs. 11.25 ± 5.05, p<0.0001). Receiver operating characteristic showed correlation of PCA3 lncRNA expression with severity of cancer. Conclusions lncRNA PCA3 expression to be linked with different stages of the disease and a prognostic indicator for disease in CML patients.
{"title":"Clinical importance of PCA3 lncRNA Aberrant expression in chronic myeloid leukemia patients: a comparative method","authors":"Ling He, Jie Zhang, Yang Peng, Hongwei Wu, Zhiqiang Sun","doi":"10.1515/tjb-2021-0114","DOIUrl":"https://doi.org/10.1515/tjb-2021-0114","url":null,"abstract":"Abstract Objectives Long noncoding RNAs (lncRNAs) plays important role in disease spread and its invasion. Overexpression of prostate cancer antigen 3 gene (PCA3gene) is reported in prostate cancer. To analyze the PCA3 lncRNA expression in chronic myeloid leukemia (CML) patients. Methods The study included clinically confirmed 100 CML patients and 100 healthy subjects. Relative quantification using Sybr Green dye was used to calculate the PCA3 lncRNAs expression. Total RNA was extracted by TRIzol method and quantitative real-time polymerase chain reaction. Results In CML patients, 9.96 ± 4.77-folds increased noncoding PCA3 lncRNA expression was observed compared to healthy subjects. Patients of chronic phase, accelerated phase, and blast crisis phase had 4.46 ± 1.36, 7.31 ± 3.10, and 12.91 ± 4.85-fold PCA3 lncRNA expression compared to healthy subjects (p<0.0001), respectively. CML patients who have a complaint of splenomegaly had higher PCA3 lncRNA expression than those who did not complain splenomegaly compared to healthy subjects (12.04 ± 5.02-fold vs. 6.09 ± 3.39-fold, p<0.0001). Patients who had ≤20,000 TLC showed fewer PCA3 lncRNA expression than those who had >20 thousand of TLC (4.45 ± 1.84 vs. 11.25 ± 5.05, p<0.0001). Receiver operating characteristic showed correlation of PCA3 lncRNA expression with severity of cancer. Conclusions lncRNA PCA3 expression to be linked with different stages of the disease and a prognostic indicator for disease in CML patients.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74176488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives The aim of this study is to determine the opinions of midwives and nurses about the efficiency and effectiveness of the biochemistry courses they took during their undergraduate education, in the education process and in their fields of study. Methods Two hundred eighty-four students studying in the department of midwifery and nursing (1–4 classes) participated in this research voluntarily. A questionnaire consisting of 10 multiple-choice questions compiled by the authors with the support of the sociodemographic data form, including age, gender, type of lycee school graduated, and the department they studied, and the literature on biochemistry education were applied to the students. Results The mean age of the students was 21.25 ± 0.12. 58.2 % of midwifery department students and 69.1 % of nursing department students reported that biochemistry education is necessary. 64.1 % of midwifery department students and 59.7 % of nursing department students stated that they thought that the biochemistry application course should be absolutely necessary. There was a significant difference between nursing and midwifery students in favor of nursing students in terms of finding the biochemistry course interesting and loving the biochemistry course. A significant difference was found between the two groups in favor of midwifery students in terms of their desire to pursue postgraduate education in biochemistry and to increase biochemistry course hours (p<0.05). Conclusions It is thought that the results of this study, which include students’ opinions, will contribute to the efficiency of biochemistry education and help the lecturer provide a new perspective.
{"title":"Examining the views of student midwives and nurses on biochemistry education","authors":"Veysel Tahiroğlu","doi":"10.1515/tjb-2022-0137","DOIUrl":"https://doi.org/10.1515/tjb-2022-0137","url":null,"abstract":"Abstract Objectives The aim of this study is to determine the opinions of midwives and nurses about the efficiency and effectiveness of the biochemistry courses they took during their undergraduate education, in the education process and in their fields of study. Methods Two hundred eighty-four students studying in the department of midwifery and nursing (1–4 classes) participated in this research voluntarily. A questionnaire consisting of 10 multiple-choice questions compiled by the authors with the support of the sociodemographic data form, including age, gender, type of lycee school graduated, and the department they studied, and the literature on biochemistry education were applied to the students. Results The mean age of the students was 21.25 ± 0.12. 58.2 % of midwifery department students and 69.1 % of nursing department students reported that biochemistry education is necessary. 64.1 % of midwifery department students and 59.7 % of nursing department students stated that they thought that the biochemistry application course should be absolutely necessary. There was a significant difference between nursing and midwifery students in favor of nursing students in terms of finding the biochemistry course interesting and loving the biochemistry course. A significant difference was found between the two groups in favor of midwifery students in terms of their desire to pursue postgraduate education in biochemistry and to increase biochemistry course hours (p<0.05). Conclusions It is thought that the results of this study, which include students’ opinions, will contribute to the efficiency of biochemistry education and help the lecturer provide a new perspective.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84568500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Galunska, A. Salim, M. Nikolova, S. Angelova, Y. Kiselova-Kaneva, S. Peev, D. Ivanova
Abstract Objectives Plaque-induced gingivitis is one of the most widely distributed periodontal disorder during childhood. The control of the pathogenic potential of the plaque is associated with oral hygiene status at individual, group, and population levels. We hypothesize that regular application of complex oral hygiene-prophylaxis could beneficially modulate salivary amino acids profile in children with different stage of plaque-induced gingivitis. Therefore, we aimed to study the salivary amino acids’ profile in relation to certain clinical indicators and environmental variables for plaque-induced gingivitis in children. Methods Fifty children (29 girls, 21 boys; mean age 8.18 ± 2.32 years) without anamnestic data for common diseases, no medication, and no data for allergy were selected. Plaque and gingival indexes were determined for assessment oral hygiene and plaque accumulation. Unstimulated whole saliva was collected, centrifuged and supernatants stored at −80 °C. Amino acid analysis was performed by liquid chromatography using analytical grade AccQ·Tag-Ultra-derivatization kit. Results Gingivitis was indicated in most of the examined children over 6 years. More than half (63.6 %) of them revealed moderate stage of the disease and a tendency to satisfactory good oral hygiene and degree of gingival inflammation. Salivary glycine, proline, arginine, serine, lysine, aspartate, glutamate, threonine, methionine, and isoleucine were higher in gingivitis children, while cysteine, tyrosine and phenylalanine decrease. In gingivitis children without regular oral hygiene-prophylaxis, some structural amino acids like glycine and proline were increased, while amino acids with protective antioxidant potential like cysteine were diminished. Conclusions Plaque-induced gingivitis is associated with increased salivary levels of certain amino acids. These may serve as distinguishing markers among children with gingivitis.
{"title":"Gingival status and prophylactic oral hygiene measures modulate salivary amino acids’ profile in children with plaque-induced gingivitis","authors":"B. Galunska, A. Salim, M. Nikolova, S. Angelova, Y. Kiselova-Kaneva, S. Peev, D. Ivanova","doi":"10.1515/tjb-2023-0107","DOIUrl":"https://doi.org/10.1515/tjb-2023-0107","url":null,"abstract":"Abstract Objectives Plaque-induced gingivitis is one of the most widely distributed periodontal disorder during childhood. The control of the pathogenic potential of the plaque is associated with oral hygiene status at individual, group, and population levels. We hypothesize that regular application of complex oral hygiene-prophylaxis could beneficially modulate salivary amino acids profile in children with different stage of plaque-induced gingivitis. Therefore, we aimed to study the salivary amino acids’ profile in relation to certain clinical indicators and environmental variables for plaque-induced gingivitis in children. Methods Fifty children (29 girls, 21 boys; mean age 8.18 ± 2.32 years) without anamnestic data for common diseases, no medication, and no data for allergy were selected. Plaque and gingival indexes were determined for assessment oral hygiene and plaque accumulation. Unstimulated whole saliva was collected, centrifuged and supernatants stored at −80 °C. Amino acid analysis was performed by liquid chromatography using analytical grade AccQ·Tag-Ultra-derivatization kit. Results Gingivitis was indicated in most of the examined children over 6 years. More than half (63.6 %) of them revealed moderate stage of the disease and a tendency to satisfactory good oral hygiene and degree of gingival inflammation. Salivary glycine, proline, arginine, serine, lysine, aspartate, glutamate, threonine, methionine, and isoleucine were higher in gingivitis children, while cysteine, tyrosine and phenylalanine decrease. In gingivitis children without regular oral hygiene-prophylaxis, some structural amino acids like glycine and proline were increased, while amino acids with protective antioxidant potential like cysteine were diminished. Conclusions Plaque-induced gingivitis is associated with increased salivary levels of certain amino acids. These may serve as distinguishing markers among children with gingivitis.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78880354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives We aimed to establish the relationship between pre-operatively measured mean platelet volume (MPV), neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR) values and early anesthetic complications like bronchospasm & laryngospasm and hypotension in patients undergoing adenotonsillar surgery and non-adenotonsillar surgeries in both pediatric and adult population. Methods Patients from both sexes aged 1–63 years, and the American Society of Anesthesiology (ASA) physical status I–II were included in the study with elective adenotonsillectomy and non-adenotonsillar surgeries. Results In total, we included 330 patients in our study. The respiratory complications observed significantly more frequent in pediatric adenotonsillar surgery group (p=0.001). When the post-operative complications were compared with the MPV, NLR and PLR values, it was observed that the MPV values were significantly higher in patients who had hypotension (p=0.01) and PLR values were significantly higher in pediatric adenotonsillectomy group who developed bronchospasm and laryngospasm. There was no relationship between other complications and the laboratory values of the groups. Conclusions In the study, we found that PLR values were significantly high in the pediatric patients having hypoxia who underwent adenotonsillectomy. MPV values were significantly high in the patients who had hypotension in the early post-operative period. Based on these findings, it has been suggested that pre-operative PLR value can be a predicting guide for bronchospasm, laryngospasm. On the other hand, MPV values can be used as a guide in terms of predicting hypotension.
{"title":"Mean platelet volume, neutrophil/lymphocyte ratio, platelet/lymphocyte ratio and early post-operative anesthesia complications","authors":"A. Altınbaş, Azime Bulut","doi":"10.1515/tjb-2023-0040","DOIUrl":"https://doi.org/10.1515/tjb-2023-0040","url":null,"abstract":"Abstract Objectives We aimed to establish the relationship between pre-operatively measured mean platelet volume (MPV), neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR) values and early anesthetic complications like bronchospasm & laryngospasm and hypotension in patients undergoing adenotonsillar surgery and non-adenotonsillar surgeries in both pediatric and adult population. Methods Patients from both sexes aged 1–63 years, and the American Society of Anesthesiology (ASA) physical status I–II were included in the study with elective adenotonsillectomy and non-adenotonsillar surgeries. Results In total, we included 330 patients in our study. The respiratory complications observed significantly more frequent in pediatric adenotonsillar surgery group (p=0.001). When the post-operative complications were compared with the MPV, NLR and PLR values, it was observed that the MPV values were significantly higher in patients who had hypotension (p=0.01) and PLR values were significantly higher in pediatric adenotonsillectomy group who developed bronchospasm and laryngospasm. There was no relationship between other complications and the laboratory values of the groups. Conclusions In the study, we found that PLR values were significantly high in the pediatric patients having hypoxia who underwent adenotonsillectomy. MPV values were significantly high in the patients who had hypotension in the early post-operative period. Based on these findings, it has been suggested that pre-operative PLR value can be a predicting guide for bronchospasm, laryngospasm. On the other hand, MPV values can be used as a guide in terms of predicting hypotension.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84987934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kerkutluoglu, H. Gunes, Ali Eren Onus, M. Dagli, O. Yucel
Abstract Objectives Advanced heart failure is the last stage of heart failure in which the life expectancy of patients is significantly reduced. Many mortality markers have been identified in advanced heart failure. Although the nesfatin-1 molecule is known as a satiety hormone, it has also been shown to be associated with many cardiovascular diseases. This study aims to elucidate the association between in-hospital mortality and nesfatin-1 level in advanced heart failure patients. Methods The research included 74 cases of advanced heart failure. During the coronary intensive care surveillance of these patients, 22 patients had in-hospital mortality. The cases, divided into groups with and without in-hospital mortality, were compared using laboratory data, echocardiography, and demographic properties. Results The age of the cases with in-hospital mortality was older than the cases without mortality [(74 (66–95) vs. 67 (26–90); p=0.019)]. Serum nesfatin-1 level and tricuspid annular plane systolic excursion (TAPSE) were statistically lower in the in-hospital mortality group (43.8 ± 5.5 vs. 40.5 ± 6.1; p=0.027, 13.5 ± 1.9 vs. 16.2 ± 2.6 p=0.001, respectively). Serum nesfatin-1 level and TAPSE were detected as independent predictors for in-hospital mortality in advanced heart failure via multivariate analysis using parameters that were significant in the univariate analysis. Receiver operator characteristic curve analysis showed that the optimum cut-off level for Nesfatin-1 in determining in-hospital mortality was ≤23.57 (pg/mL) with a specificity of 73.1 % and a sensitivity of 77.3 % (AUC=0.763, 95 % CI=0.647–0.879, p<0.001). Conclusions This research revealed that in advanced heart failure patients, serum nesfatin-1 amounts are associated with mortality and seem to be an independent predictor of mortality.
{"title":"Predictive value of nesfatin-1 in heart failure mortality","authors":"M. Kerkutluoglu, H. Gunes, Ali Eren Onus, M. Dagli, O. Yucel","doi":"10.1515/tjb-2022-0227","DOIUrl":"https://doi.org/10.1515/tjb-2022-0227","url":null,"abstract":"Abstract Objectives Advanced heart failure is the last stage of heart failure in which the life expectancy of patients is significantly reduced. Many mortality markers have been identified in advanced heart failure. Although the nesfatin-1 molecule is known as a satiety hormone, it has also been shown to be associated with many cardiovascular diseases. This study aims to elucidate the association between in-hospital mortality and nesfatin-1 level in advanced heart failure patients. Methods The research included 74 cases of advanced heart failure. During the coronary intensive care surveillance of these patients, 22 patients had in-hospital mortality. The cases, divided into groups with and without in-hospital mortality, were compared using laboratory data, echocardiography, and demographic properties. Results The age of the cases with in-hospital mortality was older than the cases without mortality [(74 (66–95) vs. 67 (26–90); p=0.019)]. Serum nesfatin-1 level and tricuspid annular plane systolic excursion (TAPSE) were statistically lower in the in-hospital mortality group (43.8 ± 5.5 vs. 40.5 ± 6.1; p=0.027, 13.5 ± 1.9 vs. 16.2 ± 2.6 p=0.001, respectively). Serum nesfatin-1 level and TAPSE were detected as independent predictors for in-hospital mortality in advanced heart failure via multivariate analysis using parameters that were significant in the univariate analysis. Receiver operator characteristic curve analysis showed that the optimum cut-off level for Nesfatin-1 in determining in-hospital mortality was ≤23.57 (pg/mL) with a specificity of 73.1 % and a sensitivity of 77.3 % (AUC=0.763, 95 % CI=0.647–0.879, p<0.001). Conclusions This research revealed that in advanced heart failure patients, serum nesfatin-1 amounts are associated with mortality and seem to be an independent predictor of mortality.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91334593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives This study aims to assess the capability of monocyte-to-lymphocyte ratio (MLR) and C-reactive protein (CRP) to diagnose and differentiate diagnosis various types of lung diseases, including non-small-cell lung cancer (NSCLC), small-cell lung cancer (SCLC) and benign pulmonary diseases (BPD). Methods Patients diagnosed with lung cancer and BPD by pathology and healthy volunteers were enrolled. Laboratory test data and clinical pathologic characteristics were recorded, including complete blood counts, CRP, NSE, CYFRA21-1 levels, age, gender, and histological type. The differences between the groups were calculated and compared. Receiver operating characteristic (ROC) analysis was performed to specify the diagnostic value of MLR and CRP in NSCLC, SCLC, and BPD. Results 2042 patients and 996 healthy volunteers were involved (NSCLC, SCLC, and BPD patients were 1,245, 302, and 495, respectively). Compared to healthy volunteers, MLR and CRP in patients with NSCLC, SCLC, and BPD were significantly higher (p<0.0001). The areas under the curve (AUC) were 0.703, 0.828, 0.784, 0.703, 0.813, and 0.798, respectively. Through the combined analysis of MLR and CRP, the AUC could be improved to 0.765, 0.882, and 0.843, respectively. Additionally, an evaluation of the diagnostic value of MLR+CRP+ NSE+CYFRA21-1 gave the AUC of 0.898 (95 % CI:0.882–0.914), 0.986 (95 % CI:0.975–0.996) and 0.925 (95 % CI:0.906–0.945), respectively. Moreover, MLR and CRP could differentiate early-stage patients (0 and I stages) from late-stage (IV stage) for NSCLC and SCLC patients, with p-values of less than 0.0001, respectively. Conclusions MLR and CRP could be good diagnostic indicators of lung diseases, especially for SCLC and BPD. Both could improve the diagnostic efficiency of traditional lung cancer biomarkers, demonstrating excellent diagnostic value, particularly in SCLC. This may supply early treatment and survival advantages for patients.
{"title":"Evaluation of the monocyte-to-lymphocyte ratio (MLR) and c-reactive protein (CRP) as diagnostic biomarkers in different lung diseases, especially for SCLC","authors":"Yue Zhang, Zhigang Xin, Qun Zhang, Zhijun Zhang, Xiao-yuan Feng","doi":"10.1515/tjb-2022-0282","DOIUrl":"https://doi.org/10.1515/tjb-2022-0282","url":null,"abstract":"Abstract Objectives This study aims to assess the capability of monocyte-to-lymphocyte ratio (MLR) and C-reactive protein (CRP) to diagnose and differentiate diagnosis various types of lung diseases, including non-small-cell lung cancer (NSCLC), small-cell lung cancer (SCLC) and benign pulmonary diseases (BPD). Methods Patients diagnosed with lung cancer and BPD by pathology and healthy volunteers were enrolled. Laboratory test data and clinical pathologic characteristics were recorded, including complete blood counts, CRP, NSE, CYFRA21-1 levels, age, gender, and histological type. The differences between the groups were calculated and compared. Receiver operating characteristic (ROC) analysis was performed to specify the diagnostic value of MLR and CRP in NSCLC, SCLC, and BPD. Results 2042 patients and 996 healthy volunteers were involved (NSCLC, SCLC, and BPD patients were 1,245, 302, and 495, respectively). Compared to healthy volunteers, MLR and CRP in patients with NSCLC, SCLC, and BPD were significantly higher (p<0.0001). The areas under the curve (AUC) were 0.703, 0.828, 0.784, 0.703, 0.813, and 0.798, respectively. Through the combined analysis of MLR and CRP, the AUC could be improved to 0.765, 0.882, and 0.843, respectively. Additionally, an evaluation of the diagnostic value of MLR+CRP+ NSE+CYFRA21-1 gave the AUC of 0.898 (95 % CI:0.882–0.914), 0.986 (95 % CI:0.975–0.996) and 0.925 (95 % CI:0.906–0.945), respectively. Moreover, MLR and CRP could differentiate early-stage patients (0 and I stages) from late-stage (IV stage) for NSCLC and SCLC patients, with p-values of less than 0.0001, respectively. Conclusions MLR and CRP could be good diagnostic indicators of lung diseases, especially for SCLC and BPD. Both could improve the diagnostic efficiency of traditional lung cancer biomarkers, demonstrating excellent diagnostic value, particularly in SCLC. This may supply early treatment and survival advantages for patients.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84082037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rand Sayed Issa, Gohar Mushtaq, A. Unnisa, A. Mahli
Abstract Objectives Leukemoid Reaction (LR) signifies leukocytosis characterized by mature neutrophils. The incidence of LR is about 1 % among hospitalized patients. Mucormycosis is a rare, aggressive, fatal fungal infection that afflicts immune-compromised patients. This study discusses the case of concomitant leukemoid reaction and mucormycosis in a patient with severe COVID-19 infection. Case presentation A 45-year-old female patient was presented to the hospital with confirmed SARS-CoV-2 and was given supplemental oxygen and placed on mechanical ventilation. Her skin biopsy revealed non-septate hyphae with wide-angle branching. Her blood tests also revealed the presence of LR. Conclusions Severe COVID-19 infection causes new-onset hyperglycemia, which can lead to metabolic acidosis, toxic metabolite accumulation in the body due to renal failure, the release of stress hormones and inflammatory cytokines, and the occurrence of secondary bacterial and opportunistic fungal infections. The study has shown that LR in severe COVID-19 may be associated with severe infections (bacterial or fungal) and other pathophysiological changes in the body.
{"title":"A case of concomitant leukemoid reaction and mucormycosis in a patient with severe COVID-19 infection","authors":"Rand Sayed Issa, Gohar Mushtaq, A. Unnisa, A. Mahli","doi":"10.1515/tjb-2023-0016","DOIUrl":"https://doi.org/10.1515/tjb-2023-0016","url":null,"abstract":"Abstract Objectives Leukemoid Reaction (LR) signifies leukocytosis characterized by mature neutrophils. The incidence of LR is about 1 % among hospitalized patients. Mucormycosis is a rare, aggressive, fatal fungal infection that afflicts immune-compromised patients. This study discusses the case of concomitant leukemoid reaction and mucormycosis in a patient with severe COVID-19 infection. Case presentation A 45-year-old female patient was presented to the hospital with confirmed SARS-CoV-2 and was given supplemental oxygen and placed on mechanical ventilation. Her skin biopsy revealed non-septate hyphae with wide-angle branching. Her blood tests also revealed the presence of LR. Conclusions Severe COVID-19 infection causes new-onset hyperglycemia, which can lead to metabolic acidosis, toxic metabolite accumulation in the body due to renal failure, the release of stress hormones and inflammatory cytokines, and the occurrence of secondary bacterial and opportunistic fungal infections. The study has shown that LR in severe COVID-19 may be associated with severe infections (bacterial or fungal) and other pathophysiological changes in the body.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"214 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75588254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Myeloperoxidase from polymorphonuclear leukocytes is an important enzyme in oxidative metabolism and has a key role in tissue injuries in oxidative stress and inflammatory conditions. Therefore, its inhibitors have become the focus of studies on new drug development in recent years. The aim of the study was to determine the inhibitory effect of organic acids on the peroxidation, chlorination, and nitration activities of myeloperoxidase. Methods Seven organic acids naturally abundant in plants were tested. Different activities of myeloperoxidase were measured in the presence of various amounts of organic acids, and inhibition rates and kinetic parameters were determined for each organic acid separately. Results All the organic acids examined had inhibitory effects on the different activities of myeloperoxidase. Comparison of the IC50 values obtained for peroxidation, chlorination, and nitration activities showed that oxalic acid was the strongest inhibitor of myeloperoxidase activity, while citric acid and succinic acid were the weakest. Conclusions The results suggested that all the organic acids examined are inhibitors of myeloperoxidase. In particular, oxalic acid and fumaric acid are popular candidates for drug development research. More studies are needed to determine the in vivo effects of organic acids and their effects in the treatment of disease.
{"title":"Inhibitory effect of organic acids on human neutrophil Myeloperoxidase’s peroxidation, chlorination, and nitration activities","authors":"B. Sarkarati","doi":"10.1515/tjb-2022-0260","DOIUrl":"https://doi.org/10.1515/tjb-2022-0260","url":null,"abstract":"Abstract Objectives Myeloperoxidase from polymorphonuclear leukocytes is an important enzyme in oxidative metabolism and has a key role in tissue injuries in oxidative stress and inflammatory conditions. Therefore, its inhibitors have become the focus of studies on new drug development in recent years. The aim of the study was to determine the inhibitory effect of organic acids on the peroxidation, chlorination, and nitration activities of myeloperoxidase. Methods Seven organic acids naturally abundant in plants were tested. Different activities of myeloperoxidase were measured in the presence of various amounts of organic acids, and inhibition rates and kinetic parameters were determined for each organic acid separately. Results All the organic acids examined had inhibitory effects on the different activities of myeloperoxidase. Comparison of the IC50 values obtained for peroxidation, chlorination, and nitration activities showed that oxalic acid was the strongest inhibitor of myeloperoxidase activity, while citric acid and succinic acid were the weakest. Conclusions The results suggested that all the organic acids examined are inhibitors of myeloperoxidase. In particular, oxalic acid and fumaric acid are popular candidates for drug development research. More studies are needed to determine the in vivo effects of organic acids and their effects in the treatment of disease.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84254450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Quercetin is a plant flavonol from the flavonoid group of polyphenols or can also be formulated as a synthetic supplement chemically. Approximately 80 % of people in Africa and other developing nations still depend on traditional herbal remedies to treat ailments. Quercetin has been demonstrated to have a variety of anticancer effects. However, the effect of quercetin on oral cancer cells remains rare. According to our systematic review, quercetin includes anti-cell viability, anti-cell survival and anti-cell proliferation. Quercetin also possesses an anti-metastatic effect by regulating the expression of epithelial-to-mesenchymal transition-related genes in oral cancer cells. The apoptotic effect of quercetin in oral cancer cells is probably via inducing cell surface death receptors, endoplasmic reticulum stress and mitochondria-mediated signaling pathways. Additionally, quercetin reduces drug resistance in KB/vincristine oral cancer cells and enhances cell sensitivity to vincristine treatment. Quercetin induces apoptosis of human oral cancer SAS cells through the endoplasmic reticulum and mitochondria-mediated signaling pathways. Quercetin inhibits cell survival and metastatic ability via the epithelial-to-mesenchymal transition-mediated signaling pathways in oral squamous cell carcinoma. Quercetin is an anti-tumour agent candidate and can also inhibit oral tumour metastasis. Indeed, the efficacy of quercetin against chemically induced oral squamous cell carcinoma remains to be elucidated.
{"title":"Quercetin induces cytotoxicity and apoptosis, reduces metastasis and drug resistance in oral cancer cells","authors":"Nan Li, Jun Wang","doi":"10.1515/tjb-2023-0003","DOIUrl":"https://doi.org/10.1515/tjb-2023-0003","url":null,"abstract":"Abstract Quercetin is a plant flavonol from the flavonoid group of polyphenols or can also be formulated as a synthetic supplement chemically. Approximately 80 % of people in Africa and other developing nations still depend on traditional herbal remedies to treat ailments. Quercetin has been demonstrated to have a variety of anticancer effects. However, the effect of quercetin on oral cancer cells remains rare. According to our systematic review, quercetin includes anti-cell viability, anti-cell survival and anti-cell proliferation. Quercetin also possesses an anti-metastatic effect by regulating the expression of epithelial-to-mesenchymal transition-related genes in oral cancer cells. The apoptotic effect of quercetin in oral cancer cells is probably via inducing cell surface death receptors, endoplasmic reticulum stress and mitochondria-mediated signaling pathways. Additionally, quercetin reduces drug resistance in KB/vincristine oral cancer cells and enhances cell sensitivity to vincristine treatment. Quercetin induces apoptosis of human oral cancer SAS cells through the endoplasmic reticulum and mitochondria-mediated signaling pathways. Quercetin inhibits cell survival and metastatic ability via the epithelial-to-mesenchymal transition-mediated signaling pathways in oral squamous cell carcinoma. Quercetin is an anti-tumour agent candidate and can also inhibit oral tumour metastasis. Indeed, the efficacy of quercetin against chemically induced oral squamous cell carcinoma remains to be elucidated.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89261767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objectives Gene expression changes during embryonic stem cell (ESC) differentiation is regulated by epigenetic mechanisms. Understanding these can help uncover how cell fate decisions are made during early embryonic development. Comparison of chromatin of ESCs with lineage-committed cells can implicate chromatin factors functional in exit from pluripotency and the choice of proper lineages. Therefore, we quantitatively analyzed histone modifications in mouse ESC differentiation towards neuroectoderm and endoderm. Methods We cultured mouse ESCs (mESCs) and differentiated them towards neuroectoderm or endoderm lineages and performed mass spectrometry on total histones. Subsequent Western blots verified significantly altered H3K36me2. RT-qPCR analyses showed changes in H3K36-specific methyltransferases, demethylases and readers at mESC stage or during neuroectoderm/endoderm commitment. Results We presented quantitative histone modification levels in mESCs and lineage-committed cells. H3K36me2 increased specifically in neuroectoderm compared to mESCs or endoderm-committed cells. Regulation of H3K36 methylation might have a role in pluripotency exit and/or differentiation. Nsd2, Dnmt3b and Zmynd11 increased during differentiation regardless of lineage. Conversely, mESCs had higher Kdm4c and Msh6 expression than differentiated cells. Comparing neuroectoderm and endoderm-committed cells, we revealed Nsd1, Setd5 and Dnmt3a had lineage specific expression pattern. Conclusions Our results show quantitative changes in histone modifications during mESC lineage commitment and implicate H3K36me2 regulation for not only pluripotency exit but also lineage choice. Its regulatory proteins show stage (mESC vs. committed) or lineage (neuroectoderm vs. endoderm) dependent expression changes. Further work will be needed to discover their possible involvement in cell fate decisions and target genes.
【摘要】目的胚胎干细胞(ESC)分化过程中基因表达变化受表观遗传机制调控。了解这些可以帮助揭示细胞命运是如何在早期胚胎发育过程中决定的。比较ESCs与谱系承诺细胞的染色质可以暗示染色质因子在多能性退出和适当谱系选择中的功能。因此，我们定量分析了小鼠ESC向神经外胚层和内胚层分化过程中的组蛋白修饰。方法培养小鼠ESCs，将其分化为神经外胚层和内胚层谱系，并对总组蛋白进行质谱分析。随后的Western blots证实H3K36me2显著改变。RT-qPCR分析显示，在mESC阶段或神经外胚层/内胚层发育期间，h3k36特异性甲基转移酶、去甲基化酶和读取器发生了变化。结果我们在mESCs和谱系承诺细胞中获得了定量的组蛋白修饰水平。与mESCs或内胚层细胞相比，H3K36me2在神经外胚层中特异性增加。H3K36甲基化的调控可能在多能性退出和/或分化中起作用。无论谱系如何，Nsd2、Dnmt3b和Zmynd11在分化过程中均有所增加。相反，mESCs的Kdm4c和Msh6表达高于分化后的细胞。比较神经外胚层细胞和内胚层细胞，我们发现Nsd1、Setd5和Dnmt3a具有谱系特异性表达模式。我们的研究结果表明，组蛋白修饰在mESC谱系承诺过程中发生了定量变化，并暗示H3K36me2调控不仅影响多能性退出，还影响谱系选择。其调节蛋白表现出阶段(mESC vs. committed)或谱系(神经外胚层vs.内胚层)依赖性表达变化。需要进一步的工作来发现它们可能参与细胞命运决定和靶基因。
{"title":"Histone proteomics implicates H3K36me2 and its regulators in mouse embryonic stem cell pluripotency exit and lineage choice","authors":"Dersu Sezginmert, Nihal Terzİ ÇİzmecİoĞlu","doi":"10.1515/tjb-2023-0030","DOIUrl":"https://doi.org/10.1515/tjb-2023-0030","url":null,"abstract":"Abstract Objectives Gene expression changes during embryonic stem cell (ESC) differentiation is regulated by epigenetic mechanisms. Understanding these can help uncover how cell fate decisions are made during early embryonic development. Comparison of chromatin of ESCs with lineage-committed cells can implicate chromatin factors functional in exit from pluripotency and the choice of proper lineages. Therefore, we quantitatively analyzed histone modifications in mouse ESC differentiation towards neuroectoderm and endoderm. Methods We cultured mouse ESCs (mESCs) and differentiated them towards neuroectoderm or endoderm lineages and performed mass spectrometry on total histones. Subsequent Western blots verified significantly altered H3K36me2. RT-qPCR analyses showed changes in H3K36-specific methyltransferases, demethylases and readers at mESC stage or during neuroectoderm/endoderm commitment. Results We presented quantitative histone modification levels in mESCs and lineage-committed cells. H3K36me2 increased specifically in neuroectoderm compared to mESCs or endoderm-committed cells. Regulation of H3K36 methylation might have a role in pluripotency exit and/or differentiation. Nsd2, Dnmt3b and Zmynd11 increased during differentiation regardless of lineage. Conversely, mESCs had higher Kdm4c and Msh6 expression than differentiated cells. Comparing neuroectoderm and endoderm-committed cells, we revealed Nsd1, Setd5 and Dnmt3a had lineage specific expression pattern. Conclusions Our results show quantitative changes in histone modifications during mESC lineage commitment and implicate H3K36me2 regulation for not only pluripotency exit but also lineage choice. Its regulatory proteins show stage (mESC vs. committed) or lineage (neuroectoderm vs. endoderm) dependent expression changes. Further work will be needed to discover their possible involvement in cell fate decisions and target genes.","PeriodicalId":23344,"journal":{"name":"Turkish Journal of Biochemistry","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86611379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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