{"title":"A new view of RNA: the 1989 discovery by Sidney Altman and Thomas Cech","authors":"M. Grigorieva, V. M. Danilova, S. Komisarenko","doi":"10.15407/ubj92.05.155","DOIUrl":"https://doi.org/10.15407/ubj92.05.155","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"155-160"},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47315439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Early in the 20th century, a number of researchers in the field of immunology investigated this science chemically. Immunochemistry is the study of antigens and antibodies and their chemical basis and resistance to disease, developed from immunology. The immunochemistry period began in 1918 and continued through the early 1960s. Since the beginning of the immunochemical period, many researchers have been working in the field of immunochemistry by introducing important immunohistochemical and immunocytochemical methods. Hsien Wu was inspired by the science of immunochemistry and was able to determine a method for the determination of hemoglobin. In this article, I attempt to illustrate Hsien’s achievements in this period by presenting Hsien Wu's scientific biography and immunochemical history. Furthermore, providing documentary and scientific information on the course of immunochemistry and the role of Hsien in this course may be a spark for some researchers to explore the reasons for some of the chemical approaches and theories of this period.
{"title":"A glance on the role of Hsien Wu in immunology development","authors":"M. Ebrahimi","doi":"10.15407/ubj92.05.161","DOIUrl":"https://doi.org/10.15407/ubj92.05.161","url":null,"abstract":"Early in the 20th century, a number of researchers in the field of immunology investigated this science chemically. Immunochemistry is the study of antigens and antibodies and their chemical basis and resistance to disease, developed from immunology. The immunochemistry period began in 1918 and continued through the early 1960s. Since the beginning of the immunochemical period, many researchers have been working in the field of immunochemistry by introducing important immunohistochemical and immunocytochemical methods. Hsien Wu was inspired by the science of immunochemistry and was able to determine a method for the determination of hemoglobin. In this article, I attempt to illustrate Hsien’s achievements in this period by presenting Hsien Wu's scientific biography and immunochemical history. Furthermore, providing documentary and scientific information on the course of immunochemistry and the role of Hsien in this course may be a spark for some researchers to explore the reasons for some of the chemical approaches and theories of this period.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"161-165"},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44932093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research on structure, mechanism and regulation of enzyme activity. Works of Nobel laureates C. Anfinsen, S. Moore, W. Stein, S. Prusiner, J. Skou, P. Boyer, J. Walker","authors":"R. P. Vynogradova, V. M. Danilova, S. Komisarenko","doi":"10.15407/ubj92.05.134","DOIUrl":"https://doi.org/10.15407/ubj92.05.134","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"134-154"},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42234430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Rezaeinasab, A. Habibi, M. Nikbakht, M. Rashno, S. Shakerian
lactate accumulation can activate the pathways of mitochondrial biogenesis in the heart muscle. the purpose of this study was to investigate the effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) inhibition and endurance training on the gene expression of lactate carriers (MCT1 and CD147) in the cardiac muscle of Stz-diabetic rats. In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after induction of diabetes with streptozotocin (STZ). The endurance training protocol was performed on a treadmill for 6 weeks. Intraperitoneal injection of DCa of 50 mg/ kg body weight was used for the inhibition of PDk4 in the myocardium. Gene expression were measured using real-time PCR. the two-way aNoVa test was used to analyze the data. the results of the study showed that after endurance training, the expression of MCT1, PDK4, and CD147 genes increased significantly in line with each other (P < 0.05), and by inhibition of PDK4 in the heart muscle, the expression of MCT1 and CD147 genes in the endurance training group + diabetes + DCA and in the diabetes group + DCA decreased significantly (P < 0.05). According to the results of this study, it can be concluded that the repeated accumulation of lactate caused by exercise training in diabetic patients decrease through mitochondrial adaptation by DCa injection and subsequently oxidative stress can be reduced in cardiac tissue of diabetic patients and heart efficacy can be increased.
{"title":"Changes in gene expression of lactate carriers (MCT1 and CD147) in cardiac muscle of diabetic male rats: the effect of dichloroacetate and endurance training","authors":"H. Rezaeinasab, A. Habibi, M. Nikbakht, M. Rashno, S. Shakerian","doi":"10.15407/ubj92.05.111","DOIUrl":"https://doi.org/10.15407/ubj92.05.111","url":null,"abstract":"lactate accumulation can activate the pathways of mitochondrial biogenesis in the heart muscle. the purpose of this study was to investigate the effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) inhibition and endurance training on the gene expression of lactate carriers (MCT1 and CD147) in the cardiac muscle of Stz-diabetic rats. In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after induction of diabetes with streptozotocin (STZ). The endurance training protocol was performed on a treadmill for 6 weeks. Intraperitoneal injection of DCa of 50 mg/ kg body weight was used for the inhibition of PDk4 in the myocardium. Gene expression were measured using real-time PCR. the two-way aNoVa test was used to analyze the data. the results of the study showed that after endurance training, the expression of MCT1, PDK4, and CD147 genes increased significantly in line with each other (P < 0.05), and by inhibition of PDK4 in the heart muscle, the expression of MCT1 and CD147 genes in the endurance training group + diabetes + DCA and in the diabetes group + DCA decreased significantly (P < 0.05). According to the results of this study, it can be concluded that the repeated accumulation of lactate caused by exercise training in diabetic patients decrease through mitochondrial adaptation by DCa injection and subsequently oxidative stress can be reduced in cardiac tissue of diabetic patients and heart efficacy can be increased.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"111-119"},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43817297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Estimation of cellular thiols metallothioneins (mts) sensitivity to continuous pressure of environmental chemical ‘cocktail’ of xenobiotics needs investigation in correct model experiments. the aim of this study was to elucidate mts contribution into bivalve mollusk response to co-exposure to xenobiotics and elevated temperature. We treated the mussels Unio tumidus Philipson, 1788 (Unionidae) with drugs diclofenac (Dc, 2 nm), nifedipine (Nf, 2 nm) or with organophosphonate herbicide glyphosate (Gl, formulation roundup maX, 79 nm) separately at 18°c and in combination at 18°c (DcNfGl) and 25°c (DcNfGl+t) during 14 days. mts were isolated from digestive gland by size-exclusion chromatography. the concentration of mts in the tissue was assessed according to metals (Zn, cu, cd) in the eluted peak of mts (mt-me) and thiols (mt-Sh) content. tissue redox status was assessed using lactate/pyruvate ratio. the assay of cells viability was based on the lysosomes ability of hemocytes to concentrate the Neutral red (Nr) dye. It was found, that mt-Sh content in the digestive gland was increased under all exposures. treatment with Dc increased the level of mt-me, whereas treatment with Gl decreased it and increased lactate/pyruvate ratio. Nf decreased this ratio by elevating pyruvate level and increased lysosomal membrane stability in hemocytes. at co-exposure to xenobiotics and elevated temperature the number of hemocytes with nuclear abnormalities was increased indicating the exceeding of organisms’ adaptive limits. multivariate statistical analyses showed negative correlations in pairs mt-Sh/mt-me and mt-Sh/pyruvate and distinguished Gl and DcNfGl+t exposed groups from other groups.
{"title":"Metallothioneins contribution to the response of bivalve mollusk to xenobiotics","authors":"V. Khoma, L. Gnatyshyna, V. Martyniuk","doi":"10.15407/ubj92.05.087","DOIUrl":"https://doi.org/10.15407/ubj92.05.087","url":null,"abstract":"Estimation of cellular thiols metallothioneins (mts) sensitivity to continuous pressure of environmental chemical ‘cocktail’ of xenobiotics needs investigation in correct model experiments. the aim of this study was to elucidate mts contribution into bivalve mollusk response to co-exposure to xenobiotics and elevated temperature. We treated the mussels Unio tumidus Philipson, 1788 (Unionidae) with drugs diclofenac (Dc, 2 nm), nifedipine (Nf, 2 nm) or with organophosphonate herbicide glyphosate (Gl, formulation roundup maX, 79 nm) separately at 18°c and in combination at 18°c (DcNfGl) and 25°c (DcNfGl+t) during 14 days. mts were isolated from digestive gland by size-exclusion chromatography. the concentration of mts in the tissue was assessed according to metals (Zn, cu, cd) in the eluted peak of mts (mt-me) and thiols (mt-Sh) content. tissue redox status was assessed using lactate/pyruvate ratio. the assay of cells viability was based on the lysosomes ability of hemocytes to concentrate the Neutral red (Nr) dye. It was found, that mt-Sh content in the digestive gland was increased under all exposures. treatment with Dc increased the level of mt-me, whereas treatment with Gl decreased it and increased lactate/pyruvate ratio. Nf decreased this ratio by elevating pyruvate level and increased lysosomal membrane stability in hemocytes. at co-exposure to xenobiotics and elevated temperature the number of hemocytes with nuclear abnormalities was increased indicating the exceeding of organisms’ adaptive limits. multivariate statistical analyses showed negative correlations in pairs mt-Sh/mt-me and mt-Sh/pyruvate and distinguished Gl and DcNfGl+t exposed groups from other groups.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"87-96"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45277805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Development of new effective drugs with low side effects and definite chemical characteristics needs identification of bioactive scaffolds for further structural optimization. New synthesized derivatives of 4-hetaryl-5-amino-1-aryl-1H-1,2,3-triazoles and 3H-[1,2,3]triazolo[4,5-b]pyridines were tested for anticancer activity using 60 human tumor cell lines within 9 cancer types. The selective influence of (5-amino-1H1,2,3-triazol-4-yl)quinazolin-4(3H)-ones: 2-(5-amino-1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl)quinazolin4(3H)-one and 2-(5-amino-1-phenyl-1H-1,2,3-triazol-4-yl)-6-bromoquinazolin-4(3H)-one on ovarian cancer OVCAR-4 cells with growth percentage (GP) = -4.08 and 6.63%, respectively, was found. The derivative 5,7-diamino-3-(3-(trifluoromethyl)phenyl)-3H-[1,2,3]triazolo[4,5-b]pyridine-6-carbonitrile possessed high activity towards lung cancer EKVX cells (GP = 29.14%). The compounds were shown to be less toxic than doxorubicin towards non-tumor human embryonic kidney cells of HEK293 line. Thus, the results of our study confirm the anticancer potential of compounds based on 5-amino-1-aryl-1H-1,2,3-triazoles scaffolds and their fused polycyclic derivatives.
{"title":"Selected 5-amino-1-aryl-1H-1,2,3-triazole scaffolds as promising antiproliferative agents","authors":"N. Pokhodylo, O. Shyyka, N. Finiuk, R. Stoika","doi":"10.15407/ubj92.05.023","DOIUrl":"https://doi.org/10.15407/ubj92.05.023","url":null,"abstract":"Development of new effective drugs with low side effects and definite chemical characteristics needs identification of bioactive scaffolds for further structural optimization. New synthesized derivatives of 4-hetaryl-5-amino-1-aryl-1H-1,2,3-triazoles and 3H-[1,2,3]triazolo[4,5-b]pyridines were tested for anticancer activity using 60 human tumor cell lines within 9 cancer types. The selective influence of (5-amino-1H1,2,3-triazol-4-yl)quinazolin-4(3H)-ones: 2-(5-amino-1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl)quinazolin4(3H)-one and 2-(5-amino-1-phenyl-1H-1,2,3-triazol-4-yl)-6-bromoquinazolin-4(3H)-one on ovarian cancer OVCAR-4 cells with growth percentage (GP) = -4.08 and 6.63%, respectively, was found. The derivative 5,7-diamino-3-(3-(trifluoromethyl)phenyl)-3H-[1,2,3]triazolo[4,5-b]pyridine-6-carbonitrile possessed high activity towards lung cancer EKVX cells (GP = 29.14%). The compounds were shown to be less toxic than doxorubicin towards non-tumor human embryonic kidney cells of HEK293 line. Thus, the results of our study confirm the anticancer potential of compounds based on 5-amino-1-aryl-1H-1,2,3-triazoles scaffolds and their fused polycyclic derivatives.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"23-32"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43852858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was studied. cryoprotective agents within the range of concentrations which provide a cryoprotective effect were found to inhibit significantly protein synthesis in cell-free systems under investigation. The most effective inhibition was exerted by polymeric cryoprotective agent PEG-400. Cryoprotective agents more efficiently inhibited protein synthesis at a cell level as compared with that in cell-free system that was likely associated with their effect on amino acids transport system. An inhibitory effect of cryoprotective agents on the protein synthesizing apparatus of cells was determined to be Mg2+-dependent and reversible.
{"title":"Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals","authors":"A. K. Gulevskyy, Yu. S. Akhatova, A. Nikolchenko","doi":"10.15407/ubj92.05.041","DOIUrl":"https://doi.org/10.15407/ubj92.05.041","url":null,"abstract":"The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was studied. cryoprotective agents within the range of concentrations which provide a cryoprotective effect were found to inhibit significantly protein synthesis in cell-free systems under investigation. The most effective inhibition was exerted by polymeric cryoprotective agent PEG-400. Cryoprotective agents more efficiently inhibited protein synthesis at a cell level as compared with that in cell-free system that was likely associated with their effect on amino acids transport system. An inhibitory effect of cryoprotective agents on the protein synthesizing apparatus of cells was determined to be Mg2+-dependent and reversible.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"41-49"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47785900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It was shown previously that inhibition of erN1 (endoplasmic reticulum to nucleus signaling 1) pathway, a central mediator of the unfolded protein response, leads to suppression of tumor growth through downregulation of key pro-proliferative and up-regulation of tumor suppressor factors and modifies the sensitivity of these genes to glucose and glutamine deprivation. However, the executive mechanisms of erN1 mediated control of glioma cell proliferation are not yet known. The goal of this study was to estimate the effect of glucose and glutamine deprivations on expression of cancer related genes in glioma U87 cells at erN1 signaling inhibition for evaluation of their possible significance in ERN1 mediated control of glioma cell proliferation. We studied the effect of glucose and glutamine deprivations on the expression level of cancer related genes encoding TMED10 (transmembrane p24 trafficking protein 10), MYL9 (myosin, light chain 9, regulatory), SPOck1 (sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1), cUl4a (cullin 4a), and cUl4B in U87 glioma control cells and cells with erN1 knockdown. It was shown that at glucose deprivation, the expression level of MYL9, SPOCK1 and CUL4B genes was significantly up-regulated in control glioma cells. ERN1 knockdown modified the sensitivity to glucose deprivation of all studied genes except TMED10 gene. At glutamine deprivation, the expression of Myl9, cUl4a and cUl4B genes was shown to be up-regulated in control glioma cells. the sensitivity of Myl9, tMeD10 and cUl4B gene expression to glutamine deprivation in glioma cells with ERN1 knockdown was significantly modified, while CUL4A and SPOCK1 gene expression did not respond to erN1 inhibition. the present study demonstrates that glucose and glutamine deprivation affected the expression of the most studied genes in a specific manner and that inhibition of ERN1 signaling preferentially modified their expression at glucose and glutamine deprivation.
{"title":"ERN1 dependent regulation of TMED10, MYL9, SPOCK1, CUL4A and CUL4B genes expression at glucose and glutamine deprivations in U87 glioma cells","authors":"O. Minchenko, O. Hnatiuk, D. O. Tsymbal","doi":"10.15407/ubj92.05.050","DOIUrl":"https://doi.org/10.15407/ubj92.05.050","url":null,"abstract":"It was shown previously that inhibition of erN1 (endoplasmic reticulum to nucleus signaling 1) pathway, a central mediator of the unfolded protein response, leads to suppression of tumor growth through downregulation of key pro-proliferative and up-regulation of tumor suppressor factors and modifies the sensitivity of these genes to glucose and glutamine deprivation. However, the executive mechanisms of erN1 mediated control of glioma cell proliferation are not yet known. The goal of this study was to estimate the effect of glucose and glutamine deprivations on expression of cancer related genes in glioma U87 cells at erN1 signaling inhibition for evaluation of their possible significance in ERN1 mediated control of glioma cell proliferation. We studied the effect of glucose and glutamine deprivations on the expression level of cancer related genes encoding TMED10 (transmembrane p24 trafficking protein 10), MYL9 (myosin, light chain 9, regulatory), SPOck1 (sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1), cUl4a (cullin 4a), and cUl4B in U87 glioma control cells and cells with erN1 knockdown. It was shown that at glucose deprivation, the expression level of MYL9, SPOCK1 and CUL4B genes was significantly up-regulated in control glioma cells. ERN1 knockdown modified the sensitivity to glucose deprivation of all studied genes except TMED10 gene. At glutamine deprivation, the expression of Myl9, cUl4a and cUl4B genes was shown to be up-regulated in control glioma cells. the sensitivity of Myl9, tMeD10 and cUl4B gene expression to glutamine deprivation in glioma cells with ERN1 knockdown was significantly modified, while CUL4A and SPOCK1 gene expression did not respond to erN1 inhibition. the present study demonstrates that glucose and glutamine deprivation affected the expression of the most studied genes in a specific manner and that inhibition of ERN1 signaling preferentially modified their expression at glucose and glutamine deprivation.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"50-61"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48120180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Kotyk, R. Iskra, O. Slivinska, N. Liubas, A. Pylypets, V. Lubenets, V. I. Pryimych, U. Lviv
ethylthiosulfanylate is alkyl ester of thiosulfoacid and belongs to the class of thiosulfonate compounds. structurally, thiosulfonates are synthetic analogues of natural phytoncides. It is known that, natural organic sulfur-containing compounds are characterized by antioxidant and detoxification properties against heavy metals toxicity. therefore, the purpose of the study was to investigate the influence of ethylthiosulfanylate, as a synthetic analogue of natural phytoncides, on the state of the pro/antioxidant system in the liver of laboratory rats exposed to cr(vI). It was found that ethylthiosulfanylate exposure at a dose 100 mg/kg body weight daily for 14 days led to a decrease in the intensity of increasing of the lipid hydroperoxides (lhP) content in the rat liver caused by cr(vI) action. In addition, ethylthiosulfanylate pretreatment prevented depletion of reduced glutathione (Gsh) pool under the action of potassium dichromate oxidative stress and performed the accumulation of cellular Gsh in rat liver.
{"title":"Effects of ethylthiosulfanylate and chromium (VI) on the state of pro/antioxidant system in rat liver","authors":"B. Kotyk, R. Iskra, O. Slivinska, N. Liubas, A. Pylypets, V. Lubenets, V. I. Pryimych, U. Lviv","doi":"10.15407/ubj92.05.078","DOIUrl":"https://doi.org/10.15407/ubj92.05.078","url":null,"abstract":"ethylthiosulfanylate is alkyl ester of thiosulfoacid and belongs to the class of thiosulfonate compounds. structurally, thiosulfonates are synthetic analogues of natural phytoncides. It is known that, natural organic sulfur-containing compounds are characterized by antioxidant and detoxification properties against heavy metals toxicity. therefore, the purpose of the study was to investigate the influence of ethylthiosulfanylate, as a synthetic analogue of natural phytoncides, on the state of the pro/antioxidant system in the liver of laboratory rats exposed to cr(vI). It was found that ethylthiosulfanylate exposure at a dose 100 mg/kg body weight daily for 14 days led to a decrease in the intensity of increasing of the lipid hydroperoxides (lhP) content in the rat liver caused by cr(vI) action. In addition, ethylthiosulfanylate pretreatment prevented depletion of reduced glutathione (Gsh) pool under the action of potassium dichromate oxidative stress and performed the accumulation of cellular Gsh in rat liver.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"78-89"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44441094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Strilbytska, T. Strutynska, U. Semaniuk, N. Burdyliyk, O. Lushchak
Nutrition affects various life-history traits. We used fruit flies Drosophila melanogaster to determine whether life-history traits, particularly life span and metabolism, are affected by dietary sucrose content. We fed flies by four different diets containing constant yeast concentration and increasing amounts of sugar ranged from 1% to 20%. We found that low sucrose diet increases female lifespan. We also showed, that low dietary sucrose maximized malate dehydrogenase, aspartate aminotransferase activity in males and lactate dehydrogenase activity in females. In addition, dietary carbohydrate has a considerable impact on urea level, suggesting that dietary carbohydrate impacts overall metabolism. Our findings revealed the influence of dietary sugar on metabolic enzymes activities, indicating an existence of optimal nutritional conditions for prolongevity phenotype and confirming an important impact of dietary sugar on life-history traits.
{"title":"Dietary sucrose defines lifespan and metabolism in Drosophila","authors":"O. Strilbytska, T. Strutynska, U. Semaniuk, N. Burdyliyk, O. Lushchak","doi":"10.15407/ubj92.05.097","DOIUrl":"https://doi.org/10.15407/ubj92.05.097","url":null,"abstract":"Nutrition affects various life-history traits. We used fruit flies Drosophila melanogaster to determine whether life-history traits, particularly life span and metabolism, are affected by dietary sucrose content. We fed flies by four different diets containing constant yeast concentration and increasing amounts of sugar ranged from 1% to 20%. We found that low sucrose diet increases female lifespan. We also showed, that low dietary sucrose maximized malate dehydrogenase, aspartate aminotransferase activity in males and lactate dehydrogenase activity in females. In addition, dietary carbohydrate has a considerable impact on urea level, suggesting that dietary carbohydrate impacts overall metabolism. Our findings revealed the influence of dietary sugar on metabolic enzymes activities, indicating an existence of optimal nutritional conditions for prolongevity phenotype and confirming an important impact of dietary sugar on life-history traits.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"97-105"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46321158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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