Indoleamine 2,3-dioxygenase (IDO) is a tryptophan-degrading enzyme belonging to the kynurenine pathway. IDO activity has been suggested as a biomarker for diagnosis of chronic kidney disease. The aim of the study was to estimate the level of IDO, urea, creatinine, uric acid, phosphate, calcium, albumin, MDA, GSH, and activity of peroxidase, catalase, arylesterase in the serum of chronic renal failure (CRF) patients treated with dialysis compared to the healthy control group. The results showed a significant increment in IDO level in patients compared with the control. Linear regression analysis using the Pearson correlation coefficient showed that increased IDO level correlates positively with urea, creatinine, uric acid, phosphate, MDA level and peroxidase activity whereas negatively with albumin, calcium, glutathione level, catalase activity and glomerular filtration rate. We concluded that IDO level might be a possible marker of oxidative stress and inflammation in patients with CRF. Keywords: 3-dioxygenase, biochemical parameters, correlation analysis, indoleamine 2, renal failure, serum
{"title":"Indoleamine 2,3-dioxygenase level and oxidative stress parameters in the serum of patients with chronic renal failure","authors":"F. M. Y. Saeed, R. F. Jasim","doi":"10.15407/ubj95.04.017","DOIUrl":"https://doi.org/10.15407/ubj95.04.017","url":null,"abstract":"Indoleamine 2,3-dioxygenase (IDO) is a tryptophan-degrading enzyme belonging to the kynurenine pathway. IDO activity has been suggested as a biomarker for diagnosis of chronic kidney disease. The aim of the study was to estimate the level of IDO, urea, creatinine, uric acid, phosphate, calcium, albumin, MDA, GSH, and activity of peroxidase, catalase, arylesterase in the serum of chronic renal failure (CRF) patients treated with dialysis compared to the healthy control group. The results showed a significant increment in IDO level in patients compared with the control. Linear regression analysis using the Pearson correlation coefficient showed that increased IDO level correlates positively with urea, creatinine, uric acid, phosphate, MDA level and peroxidase activity whereas negatively with albumin, calcium, glutathione level, catalase activity and glomerular filtration rate. We concluded that IDO level might be a possible marker of oxidative stress and inflammation in patients with CRF. Keywords: 3-dioxygenase, biochemical parameters, correlation analysis, indoleamine 2, renal failure, serum","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. D. Startseva, D. M. Hodyna, I. V. Semenyuta, O. P. Tarasyuk, S. P. Rogalsky, L. O. Metelytsia
Evaluation of undecylenic acid (UA) and its tertiary amide N,N-dibutylundecenamide (DBUA) activity in vitro against the standard and antibiotic-resistant Escherichia coli and Staphylococcus aureus strains was carried out. The antibacterial potential of the acid and its amide at 2.5 and 5.0 μM concentration both against gram-positive bacteria (S. aureus) and gram-negative (E. coli) cultures was confirmed by monitoring the diameter of the bacterial growth inhibition zones. The docking study identified methionine aminopeptidase (MAP) as the most energy-favorable potential biotarget associated with the drug resistance of E. coli and S. aureus with a binding energy in the range from -8.0 to -8.5 kcal/mol. The ligands complexation was due to the formation of hydrogen bonds with ASP108, HIS171, HIS178, GLU204, GLU235, HIS76, ASP104, GLU233, ASP93 and metal-acceptor interactions with Co2+. Overall, the results indicated that UA and DBUA activity against antibiotic-resistant strains creates prospects for the development of new antibacterial formulations. Keywords: Escherichia coli, methionine aminopeptidase, molecular docking, Staphylococcus aureus, tertiary amide, undecylenic acid
{"title":"Undecylenic acid and N,N-dibutylundecenamide as effective antibacterials against antibiotic-resistant strains","authors":"Y. D. Startseva, D. M. Hodyna, I. V. Semenyuta, O. P. Tarasyuk, S. P. Rogalsky, L. O. Metelytsia","doi":"10.15407/ubj95.04.055","DOIUrl":"https://doi.org/10.15407/ubj95.04.055","url":null,"abstract":"Evaluation of undecylenic acid (UA) and its tertiary amide N,N-dibutylundecenamide (DBUA) activity in vitro against the standard and antibiotic-resistant Escherichia coli and Staphylococcus aureus strains was carried out. The antibacterial potential of the acid and its amide at 2.5 and 5.0 μM concentration both against gram-positive bacteria (S. aureus) and gram-negative (E. coli) cultures was confirmed by monitoring the diameter of the bacterial growth inhibition zones. The docking study identified methionine aminopeptidase (MAP) as the most energy-favorable potential biotarget associated with the drug resistance of E. coli and S. aureus with a binding energy in the range from -8.0 to -8.5 kcal/mol. The ligands complexation was due to the formation of hydrogen bonds with ASP108, HIS171, HIS178, GLU204, GLU235, HIS76, ASP104, GLU233, ASP93 and metal-acceptor interactions with Co2+. Overall, the results indicated that UA and DBUA activity against antibiotic-resistant strains creates prospects for the development of new antibacterial formulations. Keywords: Escherichia coli, methionine aminopeptidase, molecular docking, Staphylococcus aureus, tertiary amide, undecylenic acid","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. V. Gudzenko, N. V. Borzova, L. D. Varbanets, I. I. Seifullina, O. E. Martsinko, E. V. Afanasenko
The search for effectors capable of influencing the catalytic activity of enzymes is an important area of modern enzymology. The aim of the study was to investigate the ability of 6 coordination compounds with malatogermanate/stannate anions and 1,10-phenanthroline cations of 3d metals to modify α-L-rhamnosidase activity of Penicillium tardum, Penicillium restrictum and Eupenicillium еrubescens strains. α-L-Rhamnosidase activity was determined by the Davis method using naringin as a substrate. It was demonstrated that [Ni(phen)3]2[{Sn(HMal)2(Mal)}Cl]•14H2O) in 0.1% concentration had the most pronounced activating effect on α-L-rhamnosidase activity of all strains studied. Noncompetitive inhibition of α-L-rhamnosidase in E. еrubescens by [Cu(phen)3]2[{Sn(HMal)2(Mal)}Cl]•10H2O was shown. The obtained results expand the idea of glycosidases possible activators and inhibitors and indicate the perspective of their use in modern biotechnological processes. Keywords: d-metals, double coordination compounds, Eupenicillium erubescens, germanium(IV), Penicillium restrictum, Penicillium tardum, stannum(IV), α-L-rhamnosidase
寻找能够影响酶的催化活性的效应器是现代酶学的一个重要领域。研究了6种具有苹果果酸盐/锡酸盐阴离子和三维金属1,10-菲罗啉阳离子的配位化合物对延迟青霉、限制青霉和丛绿绿青霉菌株α- l -鼠李糖苷酶活性的影响。以柚皮苷为底物，采用Davis法测定α- l -鼠李糖苷酶活性。结果表明，0.1%浓度的[Ni(phen)3]2[{Sn(HMal)2(Mal)}Cl]•14H2O]对各菌株α- l -鼠李糖苷酶活性的激活作用最为显著。结果表明，[Cu(phen)3]2[{Sn(HMal)2(Mal)}Cl]•10H2O对红花α- l -鼠李糖苷酶具有非竞争性抑制作用。所获得的结果扩展了糖苷酶可能的激活剂和抑制剂的想法，并指出了它们在现代生物技术过程中的应用前景。关键词:d金属，双配位化合物，绿绿青霉，锗，限制青霉，延迟青霉，锡，α- l -鼠李糖苷酶
{"title":"The influence of coordination compounds with malatogermanate/stannate anions and 1,10-phenanthroline cations of 3D metals on α-L-rhamnosidase activity of Penicillium tardum, Penicillium restrictum and Eupenicillium erubescens","authors":"O. V. Gudzenko, N. V. Borzova, L. D. Varbanets, I. I. Seifullina, O. E. Martsinko, E. V. Afanasenko","doi":"10.15407/ubj95.04.046","DOIUrl":"https://doi.org/10.15407/ubj95.04.046","url":null,"abstract":"The search for effectors capable of influencing the catalytic activity of enzymes is an important area of modern enzymology. The aim of the study was to investigate the ability of 6 coordination compounds with malatogermanate/stannate anions and 1,10-phenanthroline cations of 3d metals to modify α-L-rhamnosidase activity of Penicillium tardum, Penicillium restrictum and Eupenicillium еrubescens strains. α-L-Rhamnosidase activity was determined by the Davis method using naringin as a substrate. It was demonstrated that [Ni(phen)3]2[{Sn(HMal)2(Mal)}Cl]•14H2O) in 0.1% concentration had the most pronounced activating effect on α-L-rhamnosidase activity of all strains studied. Noncompetitive inhibition of α-L-rhamnosidase in E. еrubescens by [Cu(phen)3]2[{Sn(HMal)2(Mal)}Cl]•10H2O was shown. The obtained results expand the idea of glycosidases possible activators and inhibitors and indicate the perspective of their use in modern biotechnological processes. Keywords: d-metals, double coordination compounds, Eupenicillium erubescens, germanium(IV), Penicillium restrictum, Penicillium tardum, stannum(IV), α-L-rhamnosidase","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. P. Vasetska, V. S. Lisovska, M. H. Prodanchuk, P. H. Zhminko
The effect of concomitant pesticides and plant growth regulators on humans is still not well understood. N-oxide-2,6-dimethylpyridine (Ivin) is the plant growth regulator known to reduce the acute toxicity of pesticides, but its protective mechanisms need to be investigated. The aim of the study was to assess the hepatoprotective ability of 2,6-dimethylpyridine N-oxide (Ivin) using a model of acute CCl4-induced hepatitis. Male Wistar Han rats received two subcutaneous CCl4 injections (0.8 ml/100 g b.w.). Oral Ivin (13 or 0.13 mg/kg) and hepatoprotector “Silybor-35” (5 mg/kg) as reference substances were administered orally one hour pre- and 2 hours post-CCl4 injection. The biochemical assay of blood plasma, estimation of lipid peroxidation products in the liver tissue and histological liver analysis were done. The results of functional tests and histomorphological studies of liver tissue demonstrated that Ivin exhibited a pronounced hepatoprotective effect, more pronounced when it was administered at a low 0.13 mg/kg dose. Calculation of the hepatoprotection efficiency index for Ivin showed that it was comparable to that for “Silybor-35”. Keywords: hepatoprotective effect, Ivin, СCl4-induced hepatitis of rats
{"title":"Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats","authors":"O. P. Vasetska, V. S. Lisovska, M. H. Prodanchuk, P. H. Zhminko","doi":"10.15407/ubj95.04.035","DOIUrl":"https://doi.org/10.15407/ubj95.04.035","url":null,"abstract":"The effect of concomitant pesticides and plant growth regulators on humans is still not well understood. N-oxide-2,6-dimethylpyridine (Ivin) is the plant growth regulator known to reduce the acute toxicity of pesticides, but its protective mechanisms need to be investigated. The aim of the study was to assess the hepatoprotective ability of 2,6-dimethylpyridine N-oxide (Ivin) using a model of acute CCl4-induced hepatitis. Male Wistar Han rats received two subcutaneous CCl4 injections (0.8 ml/100 g b.w.). Oral Ivin (13 or 0.13 mg/kg) and hepatoprotector “Silybor-35” (5 mg/kg) as reference substances were administered orally one hour pre- and 2 hours post-CCl4 injection. The biochemical assay of blood plasma, estimation of lipid peroxidation products in the liver tissue and histological liver analysis were done. The results of functional tests and histomorphological studies of liver tissue demonstrated that Ivin exhibited a pronounced hepatoprotective effect, more pronounced when it was administered at a low 0.13 mg/kg dose. Calculation of the hepatoprotection efficiency index for Ivin showed that it was comparable to that for “Silybor-35”. Keywords: hepatoprotective effect, Ivin, СCl4-induced hepatitis of rats","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N-methyl-D-aspartate receptors (NMDARs), are one of the major ionotropic glutamate receptors found in excitatory synapses which play a key role in glutamatergic synaptic transmission. The receptors are regulated by post translational modifications such as phosphorylation. One of the major receptor subunits is GluN2A which is likely to get phosphorylated in vitro at a putative site Ser1291. However, the regulation of phosphorylation of this site by kinases and phosphatases is not yet completely understood. In the present study, we have used the fusion constructs of GluN2A tagged with glutathione S-transferase (GST) as substrate for phosphorylation, purified calcium/calmodulin dependent protein kinase type II (CaMKII) and radioactive P32. We demonstrated that the site phosphorylated by αCaMKII on GluN2A was Ser1291 and that protein phosphatases 1, 2A and 2C were able to dephosphorylate this phospho-GST-GluN2A-Ser1291 in vitro. In the rat brain tissue post synaptic density and cytosolic fraction the major phosphatase responsible for dephosphorylating phospho-GluN2A-Ser1291 was protein phosphatase 1. Keywords: CaMKII, cytosol, GluN2A-Ser1291 subunite, N-methyl-D-aspartate receptors, protein phosphatase 1, rat brain
n -甲基- d -天冬氨酸受体(NMDARs)是存在于兴奋性突触中的主要嗜离子型谷氨酸受体之一，在谷氨酸能突触传递中起关键作用。受体受磷酸化等翻译后修饰的调控。其中一个主要的受体亚基是GluN2A，在体外可能在一个假定的Ser1291位点被磷酸化。然而，激酶和磷酸酶对该位点磷酸化的调控尚不完全清楚。在本研究中，我们使用谷胱甘肽s -转移酶(GST)标记的GluN2A融合构建物作为磷酸化底物，纯化钙/钙调素依赖性蛋白激酶II型(CaMKII)和放射性P32。我们证明了αCaMKII在GluN2A上磷酸化的位点是Ser1291，蛋白磷酸酶1、2A和2C能够在体外使这个磷酸化的gst -GluN2A-Ser1291去磷酸化。在大鼠脑组织突触后密度和细胞质部分中，负责磷酸化glun2a - ser1291的主要磷酸酶是蛋白磷酸酶1。关键词:CaMKII，细胞质，GluN2A-Ser1291亚基，n -甲基- d -天冬氨酸受体，蛋白磷酸酶1，大鼠脑
{"title":"Ser-Thr phosphatases in the rat brain that dephosphorylate phospho-Ser(1291)-GluN2A subunit of glutamate receptor","authors":"R. R. Prabhu","doi":"10.15407/ubj95.04.010","DOIUrl":"https://doi.org/10.15407/ubj95.04.010","url":null,"abstract":"N-methyl-D-aspartate receptors (NMDARs), are one of the major ionotropic glutamate receptors found in excitatory synapses which play a key role in glutamatergic synaptic transmission. The receptors are regulated by post translational modifications such as phosphorylation. One of the major receptor subunits is GluN2A which is likely to get phosphorylated in vitro at a putative site Ser1291. However, the regulation of phosphorylation of this site by kinases and phosphatases is not yet completely understood. In the present study, we have used the fusion constructs of GluN2A tagged with glutathione S-transferase (GST) as substrate for phosphorylation, purified calcium/calmodulin dependent protein kinase type II (CaMKII) and radioactive P32. We demonstrated that the site phosphorylated by αCaMKII on GluN2A was Ser1291 and that protein phosphatases 1, 2A and 2C were able to dephosphorylate this phospho-GST-GluN2A-Ser1291 in vitro. In the rat brain tissue post synaptic density and cytosolic fraction the major phosphatase responsible for dephosphorylating phospho-GluN2A-Ser1291 was protein phosphatase 1. Keywords: CaMKII, cytosol, GluN2A-Ser1291 subunite, N-methyl-D-aspartate receptors, protein phosphatase 1, rat brain","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. S. Korolova, O. V. Hornytska, A. S. Lavrik, N. M. Druzhyna, N. Prysyazhna, T. M. Platonova
Obesity is a complex metabolic disorder that can be followed by blood coagulation disorders, atherosclerosis and atherothrombosis. In the present work, the levels of fibrinogen, soluble fibrin, D-dimer as well as protein C were measured in the blood plasma of 24 morbidly obese patients (the body mass index exceeds 40 kg/m2) to evaluate the risk of prothrombotic state. The study showed that near by 80% of patients had substantially increased fibrinogen concentration, 33% had increased concentration of soluble fibrin, 42% had increased level of D-dimer in blood plasma as compared to control. According to the results of individual analysis, the high level of fibrinogen and soluble fibrin while reduced protein C indicated the threat of thrombosis, which requires complex diagnostics to be identified. Therefore, simultaneous quantification of hemostatic system biomarkers in the blood plasma is the confident way to predict the risk of thrombotic complications in morbidly obese patients. Keywords: D-dimer, hemostasis, obesity, protein C, soluble fibrin, thrombosis
{"title":"Characterization of the blood coagulation system in morbidly obese patients","authors":"D. S. Korolova, O. V. Hornytska, A. S. Lavrik, N. M. Druzhyna, N. Prysyazhna, T. M. Platonova","doi":"10.15407/ubj95.04.003","DOIUrl":"https://doi.org/10.15407/ubj95.04.003","url":null,"abstract":"Obesity is a complex metabolic disorder that can be followed by blood coagulation disorders, atherosclerosis and atherothrombosis. In the present work, the levels of fibrinogen, soluble fibrin, D-dimer as well as protein C were measured in the blood plasma of 24 morbidly obese patients (the body mass index exceeds 40 kg/m2) to evaluate the risk of prothrombotic state. The study showed that near by 80% of patients had substantially increased fibrinogen concentration, 33% had increased concentration of soluble fibrin, 42% had increased level of D-dimer in blood plasma as compared to control. According to the results of individual analysis, the high level of fibrinogen and soluble fibrin while reduced protein C indicated the threat of thrombosis, which requires complex diagnostics to be identified. Therefore, simultaneous quantification of hemostatic system biomarkers in the blood plasma is the confident way to predict the risk of thrombotic complications in morbidly obese patients. Keywords: D-dimer, hemostasis, obesity, protein C, soluble fibrin, thrombosis","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135982591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An increase in the prevalence of overweight and obese adolescents is often combined with a low level of vitamin D that may be associated with lipid and carbohydrate metabolism disorders, which underlie the development of arterial hypertension and cardiometabolic changes. This work aimed to determine the association between vitamin D status and the main parameters of lipid and carbohydrate metabolism, and anthropometric measurements. A total of 196 adolescents (129 boys and 67 girls) from the Ternopil region were examined. Their mean age was 15.5 ± 2.3 years. Based on the body mass index (BMI): 60 adolescents had normal body weight, 60 were overweight, and 76 were obese. Anthropometric measurements and general examinations were performed. In blood serum 25(OH)D levels and the main parameters of lipid and carbohydrate metabolism were determined. Low levels of 25(OH)D were found. Prevalence of vitamin D deficiency among adolescents with BMI up to the 85th percentile was 56.7%, with BMI in the 85-97th percentiles was 70.0% and with BMI over the 97th percentile was 77.6%. Serum 25(OH)D levels in overweight and obese adolescents had a significant correlation with BMI (r = -0.427, P < 0.001), waist circumference (r = -0.462, P < 0.001), highdensity lipoprotein cholesterol (HDL-C, r = 0.214, P = 0.023), low-density lipoprotein cholesterol (LDL-C, r = -0.226, P = 0.011), atherogenic index (r = -0.284, P = 0.001), insulin level (r = -0.483, P < 0.001), and HOMA-IR index (r = -0.454, P < 0.001). We concluded that the development of cardiometabolic disorders in overweight and obese adolescents is associated with vitamin D status.
超重和肥胖青少年患病率的增加往往与维生素D水平低相结合，维生素D水平低可能与脂质和碳水化合物代谢紊乱有关，而脂质和碳水化合物代谢紊乱是动脉高血压和心脏代谢变化的基础。这项工作旨在确定维生素D状态与脂质和碳水化合物代谢主要参数以及人体测量值之间的关系。共有来自捷尔诺比尔地区的196名青少年(129名男孩和67名女孩)接受了检查。平均年龄15.5±2.3岁。根据体重指数(BMI): 60名青少年体重正常，60名超重，76名肥胖。进行了人体测量和一般检查。测定血清25(OH)D水平及脂质、碳水化合物代谢主要指标。25(OH)D水平较低。BMI在第85百分位数以下的青少年中维生素D缺乏症的患病率为56.7%，BMI在85-97百分位数之间的为70.0%，BMI在97百分位数以上的为77.6%。超重和肥胖青少年血清25(OH)D水平与BMI (r = -0.427, P < 0.001)、腰围(r = -0.462, P < 0.001)、高密度脂蛋白胆固醇(r = 0.214, P = 0.023)、低密度脂蛋白胆固醇(r = -0.226, P = 0.011)、动脉粥样硬化指数(r = -0.284, P = 0.001)、胰岛素水平(r = -0.483, P < 0.001)、HOMA-IR指数(r = -0.454, P < 0.001)有显著相关性。我们的结论是，超重和肥胖青少年的心脏代谢紊乱与维生素D水平有关。
{"title":"The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents","authors":"H. Pavlyshyn, Anna-Mariia Shulhai","doi":"10.15407/UBJ93.02.072","DOIUrl":"https://doi.org/10.15407/UBJ93.02.072","url":null,"abstract":"An increase in the prevalence of overweight and obese adolescents is often combined with a low level of vitamin D that may be associated with lipid and carbohydrate metabolism disorders, which underlie the development of arterial hypertension and cardiometabolic changes. This work aimed to determine the association between vitamin D status and the main parameters of lipid and carbohydrate metabolism, and anthropometric measurements. A total of 196 adolescents (129 boys and 67 girls) from the Ternopil region were examined. Their mean age was 15.5 ± 2.3 years. Based on the body mass index (BMI): 60 adolescents had normal body weight, 60 were overweight, and 76 were obese. Anthropometric measurements and general examinations were performed. In blood serum 25(OH)D levels and the main parameters of lipid and carbohydrate metabolism were determined. Low levels of 25(OH)D were found. Prevalence of vitamin D deficiency among adolescents with BMI up to the 85th percentile was 56.7%, with BMI in the 85-97th percentiles was 70.0% and with BMI over the 97th percentile was 77.6%. Serum 25(OH)D levels in overweight and obese adolescents had a significant correlation with BMI (r = -0.427, P < 0.001), waist circumference (r = -0.462, P < 0.001), highdensity lipoprotein cholesterol (HDL-C, r = 0.214, P = 0.023), low-density lipoprotein cholesterol (LDL-C, r = -0.226, P = 0.011), atherogenic index (r = -0.284, P = 0.001), insulin level (r = -0.483, P < 0.001), and HOMA-IR index (r = -0.454, P < 0.001). We concluded that the development of cardiometabolic disorders in overweight and obese adolescents is associated with vitamin D status.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67022133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inflammation is the common mechanism of diseases (CMD) in COVID-19 disease during pregnancy and in gestational diabetes mellitus","authors":"S. Vari","doi":"10.15407/UBJ93.02.005","DOIUrl":"https://doi.org/10.15407/UBJ93.02.005","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"93 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45118857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The discovery of the mechanisms of biological synthesis of nucleic acids: 1959 Nobel laureates S. Ochoa and A. Kornberg","authors":"O. Matyshevska, V. M. Danilova, S. Komisarenko","doi":"10.15407/UBJ93.01.129","DOIUrl":"https://doi.org/10.15407/UBJ93.01.129","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"93 1","pages":"129-138"},"PeriodicalIF":0.0,"publicationDate":"2021-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41608279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Skachkova, O. Gorbach, M. Inomistova, L. Garmanchuk, N. Khranovska
{"title":"Comparative characteristic of lung cancer stem-like cells generated in vitro under different culture conditions","authors":"O. Skachkova, O. Gorbach, M. Inomistova, L. Garmanchuk, N. Khranovska","doi":"10.15407/UBJ93.01.088","DOIUrl":"https://doi.org/10.15407/UBJ93.01.088","url":null,"abstract":"","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"93 1","pages":"88-95"},"PeriodicalIF":0.0,"publicationDate":"2021-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46360580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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