Veterinary Medicine International最新文献

Enterobacteriaceae that produce extended-spectrum β-lactamases (ESBLs) can result in severe human infections, contributing to the development of complex diseases. Klebsiella pneumoniae is one of the ESBL-producing pathogens that helps to set antimicrobial resistance as a major public health problem worldwide. The current study aimed to isolate, identify, and characterize ESBL-producing K. pneumoniae and their antimicrobial resistance pattern in retail cattle meat samples. A comprehensive set of 225 cattle meat samples was gathered from 13 upazilas within the Sylhet district of Bangladesh. The bacterial isolates were obtained through biochemical and cultural techniques, and the identification of K. pneumoniae was accomplished using polymerase chain reactions (PCRs). Antimicrobial susceptibilities were assessed using disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI, 2020) guidelines. Genes encoding ESBL enzymes were detected by the double-disk synergy test (DDST) and multiplex PCR. The overall prevalence of Klebsiella spp. was 28.89% (65/225), whereas the positive percentage of K. pneumoniae was 59.2% (29/49) confirmed by PCR. Antimicrobial resistance was observed against 12 antibiotics. According to the phenotypic resistance pattern determined through the disk diffusion method, all isolates (100%) were resistant to ampicillin, amoxicillin, cefuroxime, cefotaxime, and colistin. On the other hand, the highest susceptibility was observed towards gentamicin (97.95%), followed by ciprofloxacin (85.71%), tetracycline (83.67%), and trimethoprim-sulfamethoxazole (81.63%). Out of the total K. pneumoniae isolates analyzed, ESBL genes were present, and the highest percentage, 82.8% (24/29), tested positive for bla _TEM genes. Interestingly, among the nine ESBL genes, six were identified in K. pneumoniae isolates, except for bla _OXA, bla _CTX-M-grp2, and MultiCase _DHA. The study's results reveal the presence of extended-spectrum β-lactamase (ESBL)-producing multidrug-resistant (MDR) K. pneumoniae in retail cattle meat samples posing a substantial public health threat.

Dry eye disease (DED) is a very common disease in dogs, especially that of immune-mediated origin, with Shih Tzu dogs being some of the most affected. In this study, ultrasound changes of the orbital lacrimal gland (LG) were described in healthy Shih Tzu dogs and those diagnosed with different intensities of DED. For training purposes, a macroscopic and ultrasonographic study of the orbits of brachycephalic dog cadavers (n = 6) was conducted. Subsequently, thirty-five eyes from 23 male and female dogs aged between two and eight years, distributed into four groups according to the Schirmer tear test (STT-1), including a control group (CG, n = 10) (STT-1 ≥15 mm/min), early DED group (DED1, n = 8) (STT-1 between 14 and 11 mm/min), moderate DED group (DED2, n = 7) (STT-1 between 10 and 6 mm/min), and severe DED group (DED3, n = 10) (STT-1 ≤5 mm/min), were used. Ultrasound evaluation of LG showed a higher occurrence of glandular tissue heterogeneity (p=0.755) and the presence of hyperechoic areas (p=0.027) in dogs with DED3. The variables measuring the major axis were lower in dogs with DED3 (p=0.002). The area and perimeter were smaller in dogs with DED3 than in CG and DED1 groups (p < 0.001) and the perimeter of the LG of dogs in the DED2 group was smaller than that of CG (p < 0.001). The LG major axis (r = 0.56), area (r = 0.66), and perimeter (r = 0.66) decreased as the STT-1 of the different groups decreased. LG ultrasound of Shih Tzu dogs is a feasible technique that allows the evaluation of characteristics of the glandular tissue. The ultrasound could identify changes in texture, echogenicity, and size in LG of Shih Tzu dogs with immune-mediated DED.

This study investigated the epidemiology of gastrointestinal (GI) parasite infections among 42 meat goat herds in Khon Kaen, Northeast Thailand, based on 913 fecal samples. The predominant parasites identified in the herd were strongyle (100.0%, 42/42), Trichuris spp. (73.8%, 31/42), Eimeria spp. (66.7%, 28/42), Moniezia spp. (64.3%, 27/42), Strongyloides spp. (38.1%, 16/42), and Paramphistomum spp. (7.1%, 3/42). Coinfection with at least two GI parasites was observed in 90.4% of the herds. Molecular analysis confirmed Haemonchus contortus and Trichostrongylus spp. as the strongyle species. The study explored parasite prevalence among animals, finding significant correlations with season, sex, age, and breed. Notably, the wet season showed increased strongyle and Eimeria spp. infections. Female animals had higher odds of strongyle infection, while younger animals (less than 1 year) were more susceptible. Conversely, animals aged over 1 year were more likely to be positive for Trichuris spp., Moniezia spp., and Eimeria spp. infections. Female animals exhibited poor body condition scores (BCS) and anemia, as indicated by the FAMACHA score and packed cell volume (PCV) levels. Correlations between age, clinical signs, hematological parameters, biochemistry, and GI parasite burdens were investigated, revealing significant associations. These findings emphasize the need for tailored intervention strategies considering seasonal variations, age, and sex differences for effective GI parasite control in meat goats. Prioritizing animals exhibiting poor BCS and elevated FAMACHA score is imperative to mitigate the deleterious impacts of GI parasitic infections on health and productivity.

The distribution, epidemiology, and socioeconomic impact of Rift Valley fever (RVF) and West Nile (WN) viruses are poorly known in areas of sub-Saharan countries like Ethiopian pastoral region. The human and livestock density in the area has increased greatly in recent years, but little work has been done on arboviral diseases and their potential impact on human and livestock health. The aim of this study was to detect the circulation of zoonotic arboviruses such as Rift Valley fever virus and West Nile viruses in the livestock population and to estimate seroprevalence in Afar pastoral area northeast Ethiopia. Cross-sectional serological survey was carried out in 736 serum samples from which cattle (224), camel (155), goats (121), sheep (144), and donkeys (92) were tested for the presence of anti-RVFV and anti-WNV IgG using a competitive enzyme-linked immunosorbent assay (c-ELISA) in two purposively selected districts of the Afar region. The present study revealed a 9.1% (95% CI = 8.86-9.29) seroprevalence of co-circulation of RVF and WNV. High 32/155 (20.7%) seroprevalence of co-circulation was seen in camels, followed by goat 14/121 (11.6%), cattle 16/224 (7.14%), and sheep 5/144 (3.5%), respectively, and higher 41/421 (9.7%) seroprevalence of co-circulation was observed in Amibara district than Haruka district. Camels were seven times (OR: 7.016, 95% CI = 2.639-18.653) more likely to be seropositive for the co-circulation than sheep (p ≤ 0.001). Livestock herds found in Amibara district were 1.2 times (OR: 1.165, 95% CI = 0.680-1.996) more likely to be seropositive for RVFV infection than those in Haruka areas. Given the co-occurrence of RVFV and WNV circulations, along with often suboptimal human and animal health surveillance in many similar areas' attention should be given. Investigation of the potential socioeconomic and health impacts of zoonotic arbovirus infections in such areas is crucial. Since both RVFV and WNFV are transmitted through a mosquito vector, avoiding mosquito bites is the primary method of prevention.

Photobiomodulation therapy, also termed as low-level laser therapy, is commonly used as an adjunctive therapy for various medical conditions in veterinary practice. The ACTIVet PRO low-level laser has been used for treatment of various nondomestic species, yet the effects of dermal attributes such as pigment, feathers, or scales have not been evaluated. The effects of low-level laser therapy with the ACTIVet PRO have been investigated in laboratory animals, including a study in rats that evaluated the passage of laser light through the skin in postmortem samples. The objective of this study was to measure the power of a low-level laser (ACTIVet PRO) after penetration through dermal tissue (∼1 mm thickness) in a variety of postmortem animal tissue. This study sought to determine the impact of fur, feathers, scales, and different pigments on the ability of the laser to penetrate. Frozen and thawed skin tissue samples from various species were placed inside a light restricted laser box and exposed to a preprogrammed laser level from a Multi Radiance ACTIVet PRO photobiomodulation (PBM) device, with a power meter to measure the light penetration through the tissue samples. Light penetration measurements via power output measurements (mW) were recorded at 7 time points (range, 1-150 sec). A Friedman test was performed to evaluate the difference of the mean tissue penetration by each species at each time point. Lighter colored specimens had higher power readings than darker colored or pigmented samples, and feathers appeared to inhibit the laser, showing minimal to no power readings on bird skin covered in covert and down feathers. There was statistically significant mean tissue penetration for all time points between the rabbit and green sea turtle (p=0.0046), the red-tailed hawk and green iguana (p=0.0046), and the red-tailed hawk and green sea turtle (p=0.000034). Overall findings found that certain skin coverings, such as feathers, appear to inhibit passage of laser light through tissue to the photo meter. Darker pigmented areas of tissue appeared to absorb the laser light, which also did not allow light passage through the tissue to the photo meter. All of this illustrates that there are differences in tissue penetration between different animal species, at least in postmortem tissues. This could necessitate adjustment of machine settings for therapeutic effect in different species, though further studies would be warranted to determine the extent to which this would be necessary. Additional studies evaluating biologically active tissues would be needed as a next step, as photobiomodulation has an effect at the cellular level and the exact amount of medical benefit is not measurable in skin samples that are separate from a living organism.

The genetic diversity of Breton horses in Brazil is a critical concern, mainly due to the small population size and low number of births per year. Given that the inbreeding was overlooked by breeders for multiple generations, we estimated the genetic diversity of this population utilizing pedigree-based measures of population diversity. A total of 1394 six-generation pedigrees representing the full population of registered Breton horses in Brazil defined a total population (TP, N = 2679), with horses born between 2000 and 2022, reproductively active and alive, as reported by the breed association, representing the reference population (RP, N = 731). Using the R package PurgeR, we estimated inbreeding coefficient (F _ped ), maternal inbreeding coefficient (F _da m ), paternal inbreeding coefficient (F _sire ), individual reproductive values, number of equivalents to complete generations (t), and unbiased ancestral inbreeding coefficient (Fa). We established the equivalent complete generations (ECG), effective population size (Ne), total number of founders (Nf), effective number of founders (Nfe), total number of ancestors (Na), effective number of ancestors (Nae), founder genomes (Ng), and the inbreeding coefficient estimated with effective population size (Ne) and generation numbers (t) (F _Ne:t ), as well as Nfe/Nae and Nfe/Ng ratios for the RP. The RP inbreeding levels have stabilized, although they are still significantly rising by generation (t), and the Nfe/Ng ratio strongly suggests genetic drift. Pedigree-based analysis demonstrates that only five stallions have sired 52.83% of the RP individuals, which along with the Nae value of 36.73 implies that the observed inbreeding can be arising from patrilines. Our results suggest that observed inbreeding is due to Popular Sire Effect, highlighting the importance of monitoring breeding schemes and genetic diversity to maintain health.

This study aimed to investigate the impact of dietary inclusion of babassu oil (BO) associated with sunflower oil (SO) on feeding behavior, water intake, and physiological parameters of feedlot lambs. Thirty-five castrated male lambs (16.6 kg ± 3.9 kg) were distributed in a randomized block design with 5 treatments (diets) and 7 replications. The tested diets were oil-free diet (OF), 45 g/kg BO (BO), 30 g/kg BO with an additional 15 g/kg SO (1.5 SO), 22.5 g/kg BO with an additional 22.5 g/kg SO (2.25 SO), and 30 g/kg SO with an additional 15 g/kg BO (3.0 SO) on dry matter (DM) basis. The experimental period lasted 60 days. Animals that received BO diet and the combination of BO with SO had lower intakes of DM and neutral detergent fiber (NDF) compared to the control diet (P < 0.05). Differences on the respiratory rate (RR) was observed between animals in the control diet and those in the diets containing SO (P=0.001), with a linear increase in RR as the levels of SO in the diets increased (P=0.004). All physiological parameters showed a time effect (P < 0.05). Animals fed with the control diet had higher water intake via drinking fountain (P=0.030) and total water intake (P=0.029) compared to animals fed with diets containing SO. In relation to SO levels, water intake via drinking fountain (P=0.002), total water intake (P=0.002), and total water intake per kg of DM ingested (P=0.001) linearly increased with the levels increase in the composition of the diets. The tested diets did not alter the feeding behavior of the feedlot lambs. However, the combination of BO with different levels of SO reduced DM and water intake via drinking fountain and RR.

Surra caused by Trypanosoma evansi (T. evansi) is widely distributed and has significant impact on equine sector and international trades. However, there are no available data about the genetic characterization of this parasite in horses in Egypt. So, the goal of this study was to study the molecular characterization of T. evansi in horses and determine the changes in hematological parameters and oxidative stress associated with T. evansi infection. A total of 12 horses were examined using PCR targeting RoTat 1.2 VSG gene, and we evaluated the changes in hematological and oxidative stress between infected and healthy animals. The results revealed a notable reduction in red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) levels in the infected horses, as compared to the control healthy group. In contrast, the infected group showed a substantial increase in mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), and mean corpuscular volume (MCV). In addition, the infected group exhibited monocytopenia, eosinopenia, and notable lymphocytopenia. Regarding oxidative stress profile, the infected horses showed decreased levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and antioxidant capacity (TAC) compared to the control group. Moreover, the PCR assay targeting RoTat 1.2 VSG gene revealed positive specific band (205 bp) in all examined samples for T. evansi and the phylogenetic analysis demonstrated that strain of this study is closely related to T. evansi isolate in horses from India (MT501210) while showed difference from sequences of other species. The results emphasize the changes in blood composition and the body's response to oxidative stress caused by T. evansi infection in horses.

Lumpy skin disease (LSD) is an economically significant viral disease because of its high morbidity and high production loss. Vaccination of cattle using LSD vaccines is a more effective disease preventive and control strategy in endemic countries such as Ethiopia. Despite high vaccination coverage, there is an increasing number of field reports of the disease outbreaks. Thus, an observational study was designed to investigate disease, characterize the disease-causing agent, and isolate the virus from a local isolate for future vaccine development. Wera Jarso and Amuru districts in North West Oromia were chosen based on outbreak occurrence. For this study skin, 13 pooled biopsy samples were collected from affected cattle. In this outbreak investigation, the morbidity rate was 6.50%, the mortality rate was 0.50%, and the case fatality rate was 7.77%. The virus was isolated from all skin samples on both lamb testis and lamb kidney primary cells and confirmed to be LSDV using conventional and real-time PCR genotyping. Therefore, after each suspected LSD outbreak, a molecular test should be carried out to confirm the cause of the disease, targeting the previously suggested RPO30 or GPCR genes. Further studies targeting more regions and outbreaks, including full genome sequencing to check for genetic differences between the field viruses and vaccine strains, are recommended.

Peste des petits ruminants (PPR), a contagious virus that infects sheep and goats, damages livestock globally. This study examined the clinical features and phylogenetic analysis of the PPR virus in Iraqi breed sheep from Al-Diwaniyah province. A clinical trial of 610 sheep from different flocks found 150 oral lesions. Special primers for RT-PCR and Mega11 for phylogenetic analysis were used to study the PPR virus nucleoprotein (N) gene. The PPR infection rate was 44.6% in 4-12 month olds (n = 33/131) and 4.8% in 36-48 month olds (n = 3/75). A 608-bp PPR virus partial N gene sequence was found in 49.3% of samples by RT-PCR. In leucine, isoleucine, proline, glycine, alanine, glutamine, asparagine, threonine, serine, arginine, and lysine codons, 25 amino acid alterations were found. The protein codon 56 alanine-valine alteration was most significant. Moving from a smaller hydrophobic amino acid to one with a bigger side chain may reduce protein stability. Steric hindrance or protein shape change from Valine's extended side chain may impact folding, stability, functionality, and interactions with other molecules. Furthermore, phylogenetic analysis showed that the Nigerian strain (MN271586) was most similar to our Iraqi strain, with 100% identity and coverage. This study found the Peste des Petits Ruminants (PPR) virus in sheep flocks in Al-Diwaniyah Governorate, Iraq, which is genetically similar to neighboring countries. PPR virus strains must be monitored and genetically characterized since N gene alterations can affect infection and propagation.