Veterinary Medicine International最新文献

This study aimed to determine the seroprevalence and molecular detection of Mycobacterium tuberculosis (M. tuberculosis) in dairy cattle using ELISA and PCR techniques. A total of 500 samples (250 blood and 250 milk) were collected from various farms in Sylhet, Bangladesh. The seroprevalence was found to be 5.6% in blood samples, with PCR confirming 1.60% and 2.80% positivity in blood and milk samples, respectively. These findings highlight the zoonotic potential and public health significance of M. tuberculosis in cattle, suggesting a need for integrated One Health surveillance.

COVID-19 pandemic is considered a global crisis that adversely impacted the world economy. The virus possessed a serious threat to different sectors including agricultural, livestock, poultry and fish sectors in both developing and developed countries. COVID-19 pandemic and the associated lockdown for a long period have not only caused enormous distress to the millions of poor and marginal farmers for saving their crops and/or livestock but also affected livestock, poultry production systems and associated value chains, nutrition, health care and labour availability. In addition, COVID-19 pandemic significantly impacted the fishery sector through disruption in fish supply and value chains and had noteworthy effects on income of fish stakeholders, especially in developing countries. In this regard, the current review discussed the impact of COVID-19 pandemic on agricultural, livestock, poultry and fish sectors.

The livestock industry faces significant challenges, with disease outbreaks being a particularly devastating issue. These diseases can disrupt the food supply chain and the livelihoods of those involved in the sector. To address this, there is a growing need to enhance the health and well-being of livestock animals, ultimately improving their performance while minimizing their environmental impact. To tackle the considerable challenge posed by disease epidemics, multiomics approaches offer an excellent opportunity for scientists, breeders, and policymakers to gain a comprehensive understanding of animal biology, pathogens, and their genetic makeup. This understanding is crucial for enhancing the health of livestock animals. Multiomic approaches, including phenomics, genomics, epigenomics, metabolomics, proteomics, transcriptomics, microbiomics, and metaproteomics, are widely employed to assess and enhance animal health. High-throughput phenotypic data collection allows for the measurement of various fitness traits, both discrete and continuous, which, when mathematically combined, define the overall health and resilience of animals, including their ability to withstand diseases. Omics methods are routinely used to identify genes involved in host-pathogen interactions, assess fitness traits, and pinpoint animals with disease resistance. Genome-wide association studies (GWAS) help identify the genetic factors associated with health status, heat stress tolerance, disease resistance, and other health-related characteristics, including the estimation of breeding value. Furthermore, the interaction between hosts and pathogens, as observed through the assessment of host gut microbiota, plays a crucial role in shaping animal health and, consequently, their performance. Integrating and analyzing various heterogeneous datasets to gain deeper insights into biological systems is a challenging task that necessitates the use of innovative tools. Initiatives like MiBiOmics, which facilitate the visualization, analysis, integration, and exploration of multiomics data, are expected to improve prediction accuracy and identify robust biomarkers linked to animal health. In this review, we discuss the details of multiomics concerning the health and well-being of livestock animals.

Brucellosis is a significant zoonotic disease with global implications for animal and human public health. This study investigated the prevalence of caprine brucellosis in 39 meat goat herds in northeastern Thailand using serological and molecular methods. Seroprevalence, determined by the modified Rose Bengal test (mRBT), was negative, indicating no detectable antibodies against Brucella. However, real-time PCR identified Brucella spp. DNA in 11 samples from 8 herds. Intraherd prevalence varied from 0.0% to 9.09%, averaging 6.73% (95% CI, 4.74-8.72). Univariate analysis revealed significant risk factors associated with brucellosis at the herd level. Larger herd size correlated with increased brucellosis odds ratio (OR: 6.30; 95% CI: 1.07-36.93; p=0.041). Herds with multiple reproductive failures, including abortion, repeat breeding, and sterile, together with weak offspring, showed higher prevalence (OR: 9.37; 95% CI: 1.17-74.84; p=0.034). Multivariable analysis identified herd sizes over thirteen as a significant risk factor (OR: 10.20; 95% CI: 1.06-97.40; p=0.044). Notably, herds where owners were aware of direct transmission risks exhibited lower infection rates (OR: 0.05; 95% CI: 0.006-0.54; p=0.012). This study underscores the complementary role of molecular techniques alongside serological tests in detecting Brucella infection accurately. The findings highlight the importance of effective herd management, reproductive health monitoring, and owner education in mitigating brucellosis transmission. Implementing robust control measures, including stringent biosecurity protocols and enhanced stakeholder awareness, is crucial for controlling brucellosis in meat goat populations.

Feline herpesvirus type 1 (FeHV-1) is a primary pathogen in cats responsible for respiratory and ocular signs. There are presently no antiviral drugs that are officially licensed for veterinary use in several countries. Consequently, veterinarians must depend on off-label antivirals designed for human use. Recent advances in virus-host cell interaction have resulted in new insights into FeHV-1 replication, establishing the importance of the PI3K/Akt axis. The aim of this study was to employ this new information to assess the efficacy of two compounds whose activities involve this pathway. The antiviral properties of miltefosine and nitazoxanide were examined using seven different concentrations, evaluating cell viability and viral titers after 24 h of infection. Furthermore, selected concentrations were supplied at different time points to investigate the influence of the timing of the addition. The best results were obtained when the drugs were added both before and after viral adsorption (in particular for nitazoxanide). Each compound was further investigated by real-time PCR, western blot, and immunofluorescence. Nitazoxanide was the most effective treatment, reducing the expression of viral glycoproteins as measured by western blot and immunofluorescence, as well as reducing the release of virions in the supernatant (measured by real-time PCR). Moreover, treatment with nitazoxanide and miltefosine was associated with a decrease in Akt phosphorylation. This work emphasized the significance of comprehending the pathways necessary for viral replication and their use in the assessment of novel and effective antivirals.

Bacterial antimicrobial resistance (AMR) is a global threat to both human and animal health. This is mainly because the same antimicrobial molecules are used for the treatment and prophylaxis of bacterial diseases in both cases, and about 60% of human pathogens are shared with animals. For effective control of AMR in any country, the current situation has to be established; this is done through surveillance exercises. In Kenya, there is scanty data on the prevailing AMR situation, especially in animals. This paper reports on AMR profiles of 54 E. coli strains isolated from chickens in a cross-sectional study, out of which 36/54 (72%) were from clinically ill chickens, 11/54 (22%) were from farm chickens, and 7/54 (9.7%) were from slaughtered chicken, respectively. All 54 isolates exhibited varying antimicrobial resistance profiles with the majority showing resistance to Ampicillin (85.22%), Tetracycline (66.7%), Co-trimoxazole (57.4%), and Streptomycin (40.7%). Very few isolates were resistant to Amoxicillin and Gentamicin (each at 3.7%), Ampicillin (11.1%), and Nalidixic acid (24.1%). A total of 44/54 (81.5%) showed multiple resistance to up to 6 antimicrobial agents. This information will augment current data on the AMR status of bacteria harbored by chickens in Kenya. It will also inform policymakers in their fight against AMR.

Wound or injury can be defined as partial or complete separation of the skin, while the healing of the wounds is defined as the sequences of processes by which the skin heals and returns to its normal appearance. This study aims to evaluate the efficacy of Laurus nobilis leaf extracts on the healing of open wounds in rabbits. Twenty-four healthy rabbits were used, divided randomly into control and treated groups, each consisting of 12 rabbits. The rabbits were caused to experience circular wound defects (2.5 cm) in diameter. All animals in the control (C) group were left without treatment while those in the Laurus nobilis leaf extract group were treated with drops of the aqueous extract of Laurus nobilis in a dose of 200 mg/kg per day for 20 days. The results of macroscopic observation revealed that all animals in both groups showed equitable viability and good appetite, no mortality occurred, and no signs of infection. In the group with the Laurus nobilis extract, we noticed a significant improvement in wound-healing activity and a reduction in the wound area at p ≤ 0.05 compared with the C group. Histopathological results at the end of the study show that the thick epidermal layer covers a mass of granulation with congested blood vessels and the dermis transforms into a denser layer gradually due to the improvements of the cellularity in the C group and also revealed a well-formed skin appearance, widespread of collagen, and fibrosis within the dermis with an appearance near the normal dermis in the group treated with Laurus nobilis leaf extract. In conclusion, the results confirmed that using Laurus nobilis methanolic extract solution in a daily dose of 200 mg/kg promotes healing of open wounds in rabbits.

Introduction: Propofol is an intravenous anesthetic administered as a bolus or continuous infusion during anesthetic induction and maintenance. Its pharmacokinetic characteristics include hepatic and extrahepatic metabolism with a rapid onset of action and short duration, which provides a smooth anesthetic induction without excitatory effects. Objective: To evaluate whether the isolated use of propofol in anesthetic induction in dogs changes the hemodynamic variables assessed via echocardiography. Study Design: Prospective clinical study. Animals: Twelve healthy dogs. Methods: The dogs were induced with propofol (dose/effect) at 3 mg/kg/minute, and echocardiographic evaluations were performed immediately before anesthetic induction (MB) and immediately after its interruption (MI), at the end of the supply of the anesthetic agent. Results: A significant reduction was observed between the values of the following hemodynamic variables: Ejection Fraction (EF%), which varied from 70% to 65% (p=0.011) between moments, and the Doppler Ejection Index (DEI), which ranged from 27.1 mL/beat/m² to 22.4 mL/beat/m² (p=0.044). The heart rate (HR) and the other studied hemodynamic variables showed no significant differences between the evaluated moments. Conclusion and Clinical Relevance: Propofol was a safe anesthetic-inducing agent, maintaining stable hemodynamic indices during anesthetic induction at the used rate.

Equine herpesviruses pose a threat to equine health and potentially cause substantial economic losses to the global equine industry. EHV outbreaks have been reported in various parts of Ethiopia and the Amhara region specifically. This study aimed to detect EHVs from suspected outbreak cases in selected districts of the Northwest Amhara region. A cross-sectional study was performed from January 2022 to July 2022 to detect EHVs from suspected outbreak cases. Clinical observation was conducted for the presumptive identification of equine herpesvirus infection, and nasopharyngeal swab samples were collected for molecular detection of the viruses for confirmation. Out of 463 donkeys observed, 23 donkeys showed clinical signs suggestive of equine herpesvirus infection. Samples from 10 suspected donkeys were further subjected to polymerase chain reaction (PCR) test, amplifying ORF30 for EHV-1 and gB for EHV-2 and EHV-5. Among the 10 donkeys tested, seven (n = 7) were positive for EHV-5. All ten (n = 10) tested donkeys were negative for EHV-1 and EHV-2. EHV-5 was detected in animals with nervous signs, respiratory signs, a combination of nervous and respiratory signs, and a combination of abortion, respiratory, and nervous signs. Generally, only EHV-5 was identified from the outbreak, and more detailed epidemiological/molecular studies should be performed to better understand its dynamics and inform preventive measures.

Marek's disease (MD) is a highly infectious poultry illness with a tendency to form tumours in peripheral nerves and internal organs of affected birds. Tumours accompany MD, mostly caused by oncogenic Gallid alpha herpesvirus 2 (MD Herpes virus serotype I). Studies on avian tumours associated with MD infection are limited in Iraq. In the presented study, the positive samples of ocular tumour were 168 out of 282 MD positive samples, which accomplished in farm suffered from an unexpectedly high mortality rate. We investigated a rapidly developed tumour mass that was observed in an MD-vaccinated layer flock that showed obvious clinical signs of MD, accompanied by forming a small lump in one eye at age 21 weeks, which developed to a big lump at week 28 of age, leading to death. The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. Histopathological examination of the ocular tumour core revealed aggregation of neoplastic cells and haemorrhage that replaced the normal eye tissue, as well as early tumour formation in internal organs such as the lung and liver. In addition, abnormal lesions are susceptible to tumours in the gizzard and spleen. To our knowledge, this is the first record of an aggressive MD virus infection-mediated ocular tumour in a layer flock in Al-Najaf province, Iraq.