Pub Date : 2022-09-21DOI: 10.25073/2588-1140/vnunst.5221
L. Ha, Hoang Thi Ngoc Tu, Cao Thi Tu Oanh, Nguyen Thuy Ngan, N. T. Quỳnh
This study was conducted to investigate the species composition of Chlorophyta and Charophyta in Bach Ma National Park, Thua Thien Hue province. Here, we identified 45 species and subspecies, belonging to 17 genera, 7 families, and 4 orders. Charophyta was the most diverse phylum, with 75.56% total species. The dominant orders and families are Desmidiales and Desmidiaceae. The most diverse genus belongs to Cosmarium (15), Euastrum (5) and Scenedesmus (5). Micrasterias foliacea Bailey ex Ralfs and Cosmarium pardalis Cohn are tropical algae, while transitional species and temperate species are also present such as Cosmarium pachydermum Lund. var. indicum Iyenf. et Vim Bai and Euastrum jenneri W. Archer, respectively. The dominant group is described as Desmidiales with Chlorococcales (Ch. I) index ranging from 0.087 to 0.19 in Thuy dien Dam and Thuy dien bridge, while there are several species of Sphaeropleales with Ch. I index of 0.67 in Hoang Yen stream.
研究了顺化省巴赫马国家公园绿藻和绿藻的种类组成。共鉴定出4目7科17属45个种和亚种。绿藻门种类最多，占总种数的75.56%。主要目和科为锦葵亚目和锦葵科。最多样化的属属是Cosmarium(15)、Euastrum(5)和Scenedesmus(5)。Micrasterias foliacea Bailey ex Ralfs和Cosmarium pardalis Cohn是热带藻类，同时也存在过渡种和温带物种，如Cosmarium pachydermum Lund。籼稻变种。et Vim Bai和Euastrum jenneri W. Archer。赤色坝和赤色桥的优势类群为绿球菌(Chlorococcales, Ch. I)指数在0.087 ~ 0.19之间的Desmidiales，黄岩河的优势类群为球藻(Sphaeropleales)， Ch. I指数为0.67。
{"title":"Species Composition Diversity of Chlorophyta and Charophyta Phyla in Bach Ma National Park, Thua Thien Hue Province","authors":"L. Ha, Hoang Thi Ngoc Tu, Cao Thi Tu Oanh, Nguyen Thuy Ngan, N. T. Quỳnh","doi":"10.25073/2588-1140/vnunst.5221","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5221","url":null,"abstract":"This study was conducted to investigate the species composition of Chlorophyta and Charophyta in Bach Ma National Park, Thua Thien Hue province. Here, we identified 45 species and subspecies, belonging to 17 genera, 7 families, and 4 orders. Charophyta was the most diverse phylum, with 75.56% total species. The dominant orders and families are Desmidiales and Desmidiaceae. The most diverse genus belongs to Cosmarium (15), Euastrum (5) and Scenedesmus (5). Micrasterias foliacea Bailey ex Ralfs and Cosmarium pardalis Cohn are tropical algae, while transitional species and temperate species are also present such as Cosmarium pachydermum Lund. var. indicum Iyenf. et Vim Bai and Euastrum jenneri W. Archer, respectively. The dominant group is described as Desmidiales with Chlorococcales (Ch. I) index ranging from 0.087 to 0.19 in Thuy dien Dam and Thuy dien bridge, while there are several species of Sphaeropleales with Ch. I index of 0.67 in Hoang Yen stream. \u0000 \u0000 ","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77978204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.25073/2588-1140/vnunst.5275
T. Thao, Kh Tam, N. T. Thom, Nguyễn Thương Tuấn
In this study, Zinc oxide nanoparticles (ZnO NPs) were green synthesized using Piper chaudocanum L. (PC) leaf extract by sol-gel method and used as an adsorbent for the removal of Pb (II) ions from aqueous solution. The synthesized ZnO NPs were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), Ultraviolet - visible spectroscopy (UV-Vis) and Brunauer - Emmet - Teller (BET). The results revealed high purity and wurtzite hexagonal structure of ZnO NPs in the size range of 28-37 nm. The morphology of the ZnO NPs varies in different polycrystalline aggregates depending on the amount of extract used, from nanorods to NPs clusters with a BET surface area of 8.56 m2g-1. Pb (II) ion adsorption was investigated at different pH, contact time, initial metal ion concentration, adsorbent dose and temperature. ZnO NPs were synthesized using 30 mL PC leaf extract (ZS30) is the best Pb (II) absorbent. The adsorption isotherm was well described by Langmuir (at low concentration of Pb (II)) and Freundlich isotherm model (at higher concentration of Pb (II)). The adsorption process follows pseudo-second-order reaction kinetic with a high regression coefficient. The calculated thermodynamic parameters, ΔGo (between - 8.699 and - 7.569 kJ/mol at 298 - 316 K), ∆Ho (-20.085 kJ/mol) showed that the adsorption of Pb (II) on ZS30 surface was feasible, spontaneous and exothermic, respectively. The first layer absorption is chemisorption while the next layers are physical adsorption.
{"title":"Green Synthesis of ZnO Nanoparticles using Piper chaudocanum L. Leaf Extract: Characterization and its Application in Pb (II) Adsorption","authors":"T. Thao, Kh Tam, N. T. Thom, Nguyễn Thương Tuấn","doi":"10.25073/2588-1140/vnunst.5275","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5275","url":null,"abstract":"In this study, Zinc oxide nanoparticles (ZnO NPs) were green synthesized using Piper chaudocanum L. (PC) leaf extract by sol-gel method and used as an adsorbent for the removal of Pb (II) ions from aqueous solution. The synthesized ZnO NPs were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), Ultraviolet - visible spectroscopy (UV-Vis) and Brunauer - Emmet - Teller (BET). The results revealed high purity and wurtzite hexagonal structure of ZnO NPs in the size range of 28-37 nm. The morphology of the ZnO NPs varies in different polycrystalline aggregates depending on the amount of extract used, from nanorods to NPs clusters with a BET surface area of 8.56 m2g-1. Pb (II) ion adsorption was investigated at different pH, contact time, initial metal ion concentration, adsorbent dose and temperature. ZnO NPs were synthesized using 30 mL PC leaf extract (ZS30) is the best Pb (II) absorbent. The adsorption isotherm was well described by Langmuir (at low concentration of Pb (II)) and Freundlich isotherm model (at higher concentration of Pb (II)). The adsorption process follows pseudo-second-order reaction kinetic with a high regression coefficient. The calculated thermodynamic parameters, ΔGo (between - 8.699 and - 7.569 kJ/mol at 298 - 316 K), ∆Ho (-20.085 kJ/mol) showed that the adsorption of Pb (II) on ZS30 surface was feasible, spontaneous and exothermic, respectively. The first layer absorption is chemisorption while the next layers are physical adsorption.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90854204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.25073/2588-1140/vnunst.5225
T. T. Huong, Nguyen Xuan Tòng
Three genes p53, rarα1, wnt and liver of Medaka fish tissue were selected to study the toxicity of DDT to fish embryo (8 days old) and adult Medaka fish (3 months old). TEM and real-time PCR methods were used to examine changes in structure of liver tissue and genetic expression. The TEM results recorded a clear difference between the control and the experimental sample. Adult Medaka fish exposed to 1µg/L DDT for 24 hours has a distorted liver cell nucleus with many hollow lipid particles, and the sinus-shaped meshes are clustered, interrupted; lysosome is not intact with many degenerative bubbles. The results of real-time PCR analysis showed that DDT affects the development, different and proliferation of cells in the embryonic stage and gene expression in adult stage. All three genes p53, rarα1 and wnt in fish embryos changed strongly and tended to be inhibited when exposuring with 1700µg/L DDT, the values were recorded respectively 0.9; 4.9 and 5.4 times compared to the control sample (1 times). For adult fish, gene expression was lower than fish embryos with real-time PCR analysis values for the three genes p53, rarα1 and wnt respectively 0.9 and 0.5; 0.36 and 0.09; 0.53 and 0.09 times after exposure to 1500 and 1700 µg/L DDT. These results demonstrated that gene expression is dependent on the developmental stage of the cell as well as on the dose of the chemical exposed. The development, cell differentiation/proliferation in the embryonic and adulthood stages affected differently to the cell gene expression.
{"title":"Evaluation of the Toxicity of Dichlorodiphenyl-trichloroethane (DDT) to the Expression Levels of Three Genes p53, rarα1, wnt in the Embryos and Liver of Fish Medaka Oryzias latipes","authors":"T. T. Huong, Nguyen Xuan Tòng","doi":"10.25073/2588-1140/vnunst.5225","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5225","url":null,"abstract":"Three genes p53, rarα1, wnt and liver of Medaka fish tissue were selected to study the toxicity of DDT to fish embryo (8 days old) and adult Medaka fish (3 months old). TEM and real-time PCR methods were used to examine changes in structure of liver tissue and genetic expression. The TEM results recorded a clear difference between the control and the experimental sample. Adult Medaka fish exposed to 1µg/L DDT for 24 hours has a distorted liver cell nucleus with many hollow lipid particles, and the sinus-shaped meshes are clustered, interrupted; lysosome is not intact with many degenerative bubbles. The results of real-time PCR analysis showed that DDT affects the development, different and proliferation of cells in the embryonic stage and gene expression in adult stage. All three genes p53, rarα1 and wnt in fish embryos changed strongly and tended to be inhibited when exposuring with 1700µg/L DDT, the values were recorded respectively 0.9; 4.9 and 5.4 times compared to the control sample (1 times). For adult fish, gene expression was lower than fish embryos with real-time PCR analysis values for the three genes p53, rarα1 and wnt respectively 0.9 and 0.5; 0.36 and 0.09; 0.53 and 0.09 times after exposure to 1500 and 1700 µg/L DDT. These results demonstrated that gene expression is dependent on the developmental stage of the cell as well as on the dose of the chemical exposed. The development, cell differentiation/proliferation in the embryonic and adulthood stages affected differently to the cell gene expression.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89584164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.25073/2588-1140/vnunst.5491
Le The Thai, Quach Hong Thai, Nguyen Thi Hong Loan
In this study, trypsin-like protease activity and expression of serine protease encoding genes (PmPPAE1, PmPPAE2 - clip-serine proteases and PmTLP1, PmTLP2 - trypsin-like proteases) in the different tissues of black tiger shrimp were determined. The expression level of PmPPAE1 and PmPPAE2 was the highest in the hemolymph; meanwhile that of PmTLP1 and PmTLP2 was the highest in the hepatopancreas. In the hemolymph of white spot syndrome virus (WSSV)-injected shrimp, the expression of PmPPAE1, PmPPAE2 and PmTLP2 genes was upregulated by 2.32, 2.57 and 4.56-folds, respectively, whereas the expression of PmTLP1 was downregulated by 1.47-folds relative to the control group (injected with phosphate buffered saline). In the hepatopancreas of WSSV-injected shrimp, the expression levels of PmPPAE1, PmPPAE2 increased by 2.24 and 3.99-folds, respectively, and that of PmTLP1 and PmTLP2 decreased by 1.51, 1.29-folds, respectively. Trypsin-like protease activity was found increased 3.25-fold and decreased 4.1-fold, in the hemolymph and hepatopancreas of WSSV-infected shrimp, respectively. These data indicate the role of serine proteases in immune response of the shrimp.
{"title":"Expression of some Serine Protease Encoding Genes and Trypsin-like Protease Activity in Black Tiger Shrimp (Penaeus monodon) Infected with White Spot Syndrome Virus","authors":"Le The Thai, Quach Hong Thai, Nguyen Thi Hong Loan","doi":"10.25073/2588-1140/vnunst.5491","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5491","url":null,"abstract":"In this study, trypsin-like protease activity and expression of serine protease encoding genes (PmPPAE1, PmPPAE2 - clip-serine proteases and PmTLP1, PmTLP2 - trypsin-like proteases) in the different tissues of black tiger shrimp were determined. The expression level of PmPPAE1 and PmPPAE2 was the highest in the hemolymph; meanwhile that of PmTLP1 and PmTLP2 was the highest in the hepatopancreas. In the hemolymph of white spot syndrome virus (WSSV)-injected shrimp, the expression of PmPPAE1, PmPPAE2 and PmTLP2 genes was upregulated by 2.32, 2.57 and 4.56-folds, respectively, whereas the expression of PmTLP1 was downregulated by 1.47-folds relative to the control group (injected with phosphate buffered saline). In the hepatopancreas of WSSV-injected shrimp, the expression levels of PmPPAE1, PmPPAE2 increased by 2.24 and 3.99-folds, respectively, and that of PmTLP1 and PmTLP2 decreased by 1.51, 1.29-folds, respectively. Trypsin-like protease activity was found increased 3.25-fold and decreased 4.1-fold, in the hemolymph and hepatopancreas of WSSV-infected shrimp, respectively. These data indicate the role of serine proteases in immune response of the shrimp.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78634675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.25073/2588-1140/vnunst.5371
Ngo Van Quang, Nguyen Ngoc Anh, Quach Thi Minh Thu, T. T. Vân, D. Cuong, Nguyen Quang Tam, Thanh Thi Thu Thuy
In this paper, the extraction conditions of ulvan from green seaweed Ulva lactuca by using Ultrasound-assisted extraction method were optimised. Experience was designed using Box-behnken model with experient areas as following: extraction temperature (X1: 50–90 °C), extraction time (X2: 20–40, min) and solvent-to-material ratio (X3: 50/1–70/1, v/w), objective function (Y: extraction yield) was required to get maximum value. The result showed that the optimization conditions for extraction were: extraction temperature 84.28 °C, extraction time 30.59 min with 60.21/1 (v/w) as solvent-to-material ratio, and the expected objective function Y is 22.86% based on dried seaweed weight.
{"title":"Optimization of Ultrasound-assisted Extraction of Ulvan from Green Seaweed Ulva lactuca","authors":"Ngo Van Quang, Nguyen Ngoc Anh, Quach Thi Minh Thu, T. T. Vân, D. Cuong, Nguyen Quang Tam, Thanh Thi Thu Thuy","doi":"10.25073/2588-1140/vnunst.5371","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5371","url":null,"abstract":"In this paper, the extraction conditions of ulvan from green seaweed Ulva lactuca by using Ultrasound-assisted extraction method were optimised. Experience was designed using Box-behnken model with experient areas as following: extraction temperature (X1: 50–90 °C), extraction time (X2: 20–40, min) and solvent-to-material ratio (X3: 50/1–70/1, v/w), objective function (Y: extraction yield) was required to get maximum value. The result showed that the optimization conditions for extraction were: extraction temperature 84.28 °C, extraction time 30.59 min with 60.21/1 (v/w) as solvent-to-material ratio, and the expected objective function Y is 22.86% based on dried seaweed weight.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76407385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-25DOI: 10.25073/2588-1140/vnunst.5461
Le Minh Thuy, Dang Thi Lieu, V. D. Nam, N. T. Ngọc, D. H. Anh, T. Tri
This study has focused on the development of the method LC-MS/MS to determine 2,2-bis(4-hydroxyphenyl)propane (bisphenol A: BPA) and 4,4’-dihydroxydiphenylmethane (bisphenol F: BPF) in indoor samples by using ultrasonication extraction and solid-phase extraction for preparation. The chromatographic procedure was performed on Kinetex C18 2.1 x 150 mm, 2.6 µm column with mobile phases: 2 mM HCOONH4 in MeOH and H2O:MeOH (90:10,…). The method quantification limits (MQL) of BPA and BPF were 15.0 and 12.0 ng/g, respectively. The mean recoveries were 80.4% for BPA and 93.8% for BPF (RSD<15%). The calibration curve for both BPA and BPF was linear over a concentration range of 1.0 to 500 ng/mL (R2 ≥ 0.999). Twenty-one samples were collected from private houses, laboratories, drug stores, and plastic recycling factories in Hanoi. BPA and BPF were found in dust samples with frequencies of 85.7 and 100%, respectively. Concentrations of BPA and BPF in dust samples were in the ranges of
本研究主要建立了液相色谱-质谱联用技术(LC-MS/MS)，通过超声提取和固相萃取制备，测定室内样品中的2,2-双(4-羟基苯基)丙烷(双酚A: BPA)和4,4 ' -二羟基二苯基甲烷(双酚F: BPF)。色谱采用Kinetex C18 2.1 x 150 mm, 2.6µm色谱柱，流动相:2mm HCOONH4 in MeOH和H2O:MeOH(90:10，…)。双酚a和双酚f的定量限(MQL)分别为15.0和12.0 ng/g。双酚a的平均回收率为80.4%，双酚f的平均回收率为93.8% (RSD<15%)。BPA和BPF在1.0 ~ 500 ng/mL范围内均呈线性关系(R2≥0.999)。从河内的私人住宅、实验室、药店和塑料回收厂收集了21个样本。BPA和BPF在粉尘样品中的检测频率分别为85.7和100%。粉尘样品中BPA和BPF的浓度分别在< MQL-2230 ng/g(平均值:626)和< mql - 149000 ng/g(平均值:14700)范围内。根据测量的浓度，估计了不同年龄组的人通过室内灰尘摄入双酚的暴露量。在塑料回收工厂工作的人的估计接触剂量最高(23.6纳克/公斤-体重/天)，但低于EPA和欧洲食品安全局的口服参考剂量(50微克/公斤-体重/天)。
{"title":"Determination of Bisphenol in Indoor Dust Samples by Liquid Chromatography in Combination with Quadrupole Mass Spectrometry (LC-MS/MS)","authors":"Le Minh Thuy, Dang Thi Lieu, V. D. Nam, N. T. Ngọc, D. H. Anh, T. Tri","doi":"10.25073/2588-1140/vnunst.5461","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5461","url":null,"abstract":"This study has focused on the development of the method LC-MS/MS to determine 2,2-bis(4-hydroxyphenyl)propane (bisphenol A: BPA) and 4,4’-dihydroxydiphenylmethane (bisphenol F: BPF) in indoor samples by using ultrasonication extraction and solid-phase extraction for preparation. The chromatographic procedure was performed on Kinetex C18 2.1 x 150 mm, 2.6 µm column with mobile phases: 2 mM HCOONH4 in MeOH and H2O:MeOH (90:10,…). The method quantification limits (MQL) of BPA and BPF were 15.0 and 12.0 ng/g, respectively. The mean recoveries were 80.4% for BPA and 93.8% for BPF (RSD<15%). The calibration curve for both BPA and BPF was linear over a concentration range of 1.0 to 500 ng/mL (R2 ≥ 0.999). Twenty-one samples were collected from private houses, laboratories, drug stores, and plastic recycling factories in Hanoi. BPA and BPF were found in dust samples with frequencies of 85.7 and 100%, respectively. Concentrations of BPA and BPF in dust samples were in the ranges of <MQL–2230 ng/g (mean: 626) and <MQL–149,000 ng/g (mean: 14,700), respectively. Based on the measured concentration, the human exposure to bisphenol via indoor dust ingestion was estimated for various age groups. The estimated exposure dose for people working at plastic recycling factories was the highest level (23.6 ng/kg-bw/day) but lower than the oral reference dose by the EPA and the European Food Safety Authority (50 µg/kg-bw/day).","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91012254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-25DOI: 10.25073/2588-1140/vnunst.5373
T. Yen, T. D. Manh, A. Mechler, N. Son, Huynh Thi Diem Uyen, Huynh Ngoc Bich, Vo Van Quan
Edaravone (EDV, 3-methyl-1-phenyl-2-pyrazolin-5-one) is a neuroprotective drug that has been used to treat acute stroke caused by cerebral thrombosis and embolism. Studies showed that EDV could be used to treat diseases related to oxidizing agents; however, the activity has not been fully studied yet. In this study, the free radical scavenging activity of EDV was investigated by thermodynamic and kinetic calculations. The results showed that EDV exhibited good HOO• radical scavenging activity in an aqueous medium at pH = 7.40 (koverall(HOO) = 8.58´107 M-1 s-1) through the electron transfer mechanism of the anion state. In contrast, this activity was insignificant in non-polar environments. EDV also exhibited excellent antiradical activity against HO·, CH3O·, CH3OO·, O2·-, NO2, SO4·-, N3· and DPPH radicals in the aqueous solution. Thus, it appears to suggest that EDV is a promising radical scavenger in polar environments.
{"title":"The Mechanisms and Kinetics of Radical Scavenging Activity of Edaravone: A Computational Insight","authors":"T. Yen, T. D. Manh, A. Mechler, N. Son, Huynh Thi Diem Uyen, Huynh Ngoc Bich, Vo Van Quan","doi":"10.25073/2588-1140/vnunst.5373","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5373","url":null,"abstract":"Edaravone (EDV, 3-methyl-1-phenyl-2-pyrazolin-5-one) is a neuroprotective drug that has been used to treat acute stroke caused by cerebral thrombosis and embolism. Studies showed that EDV could be used to treat diseases related to oxidizing agents; however, the activity has not been fully studied yet. In this study, the free radical scavenging activity of EDV was investigated by thermodynamic and kinetic calculations. The results showed that EDV exhibited good HOO• radical scavenging activity in an aqueous medium at pH = 7.40 (koverall(HOO) = 8.58´107 M-1 s-1) through the electron transfer mechanism of the anion state. In contrast, this activity was insignificant in non-polar environments. EDV also exhibited excellent antiradical activity against HO·, CH3O·, CH3OO·, O2·-, NO2, SO4·-, N3· and DPPH radicals in the aqueous solution. Thus, it appears to suggest that EDV is a promising radical scavenger in polar environments.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85657293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-09DOI: 10.25073/2588-1140/vnunst.5184
P. Oanh, D. Xuyen, N. Thanh
The vegetation diversity at Cham Chu nature reserve has two vegetation type groups at different heights (upper and under 700 m). The vegetation type group in upper 700 m with 4 types are subtropical evergreen closed forest (I), closed evergreen tropical rain forest on limestone mountains (II), closed forest mixed with broadleaf and coniferous trees (III), and tropical evergreen sparse forest that regeneration after exploiting (IV). The vegetation type group under 700 m with 3 types are closed evergreen tropical rain forest on lowland (V), closed evergreen tropical rain forest on limestone mountains (VI) and secondary vegetation that impacts by human (VII).
{"title":"Study on Vegetation at Cham Chu Nature Reserve, Tuyen Quang Province","authors":"P. Oanh, D. Xuyen, N. Thanh","doi":"10.25073/2588-1140/vnunst.5184","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5184","url":null,"abstract":"The vegetation diversity at Cham Chu nature reserve has two vegetation type groups at different heights (upper and under 700 m). The vegetation type group in upper 700 m with 4 types are subtropical evergreen closed forest (I), closed evergreen tropical rain forest on limestone mountains (II), closed forest mixed with broadleaf and coniferous trees (III), and tropical evergreen sparse forest that regeneration after exploiting (IV). The vegetation type group under 700 m with 3 types are closed evergreen tropical rain forest on lowland (V), closed evergreen tropical rain forest on limestone mountains (VI) and secondary vegetation that impacts by human (VII).","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73855784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-09DOI: 10.25073/2588-1140/vnunst.5199
P. Lưu, Tran Thi Hoang Yen, H. Thuy, Tu Thi Cam Loan
Cyanobacterial blooms in eutrophic waters pose serious threats to public health, water quality, and ecological management worldwide. Mitigation of the problem is essential to maintain stainable development. In this study, the effectiveness of local soils collected from the Tri An Reservoir (TAR) lakeside in the removal of cyanobacteria Microcystis aeruginosa was investigated. The organic matter content and clay mineral were characterized and used for the experiment. Cell density, percentage of growth inhibition, and changes in cell morphology were observed and used at endpoints of the experiment. The three soil samples named TA1, TA2, and TA3 were characterized with a high proportion of illit, montmorillonit, and kaolinite, respectively. Our results indicated that different soil compositions generated different inhibition on the growth of M. aeruginosa. Exposure to the soil high in kaolinite (52.1%) at a dose of 500 mg/L completely inhibited M. Aeruginosa growth, while the soil contained a high amount of montmorillonit (42.3%) inhibited only 38.4% of the growth of M. aeruginosa. The half-maximal effective concentration (EC50) values of the TA1, TA2, and TA3 soil samples were 351.6 mg/L, 668.9 mg/L, 201.3 mg/L, respectively. Our results suggested that soil rich in kaolinite can be used to prevent harmful cyanobacterial blooms in inland waters.
{"title":"Mitigation of Cyanobacteria Isolated from Tri An Reservoir Using Local Soils","authors":"P. Lưu, Tran Thi Hoang Yen, H. Thuy, Tu Thi Cam Loan","doi":"10.25073/2588-1140/vnunst.5199","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5199","url":null,"abstract":"Cyanobacterial blooms in eutrophic waters pose serious threats to public health, water quality, and ecological management worldwide. Mitigation of the problem is essential to maintain stainable development. In this study, the effectiveness of local soils collected from the Tri An Reservoir (TAR) lakeside in the removal of cyanobacteria Microcystis aeruginosa was investigated. The organic matter content and clay mineral were characterized and used for the experiment. Cell density, percentage of growth inhibition, and changes in cell morphology were observed and used at endpoints of the experiment. The three soil samples named TA1, TA2, and TA3 were characterized with a high proportion of illit, montmorillonit, and kaolinite, respectively. Our results indicated that different soil compositions generated different inhibition on the growth of M. aeruginosa. Exposure to the soil high in kaolinite (52.1%) at a dose of 500 mg/L completely inhibited M. Aeruginosa growth, while the soil contained a high amount of montmorillonit (42.3%) inhibited only 38.4% of the growth of M. aeruginosa. The half-maximal effective concentration (EC50) values of the TA1, TA2, and TA3 soil samples were 351.6 mg/L, 668.9 mg/L, 201.3 mg/L, respectively. Our results suggested that soil rich in kaolinite can be used to prevent harmful cyanobacterial blooms in inland waters.","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82662730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-31DOI: 10.25073/2588-1140/vnunst.5456
Nguyen Thi Ngoc Anh, Nguyen Huu Duc Ton, Dinh Minh Quang
Ellochelon vaigiensis (Quoy & Gaimard, 1825) distributes widely from marine to brackish and freshwater in the Indo-Pacific region, including Vietnam. In the Mekong Delta, this fish distributes mainly in the estuaries and is a commercial value fish species. However, information about this fish digestive tract morphology and energy storage capacity had been still unknown. These characteristics, after being elucidated, wil be the basis for further research on artificially rearing them, contributing to the diversity of cultured subjects. This study was carried out in several coastal areas of provinces in the Mekong Delta from Ben Tre to Tra Vinh, Soc Trang and Bac Lieu. The analysis results of a total of 656 samples (289 females and 367 males) collected monthly, from March to October 2021, showed that E. vaigiensis had a small mouth, thick gill comb, short stomach and long intestines. This showed that they belong to the group of herbivorous fish. Ellochelon vaigiensis displayed a high energy storage capacity due to its relatively large Clark coefficient (0.73±0.03 SE). This value in the wet season (0.66±0.01 SE) is lower than in the dry season (0.85±0.01 SE, t=16.98, p<0.01). This value changed significantly according to the study sites (1-way ANOVA, F=4.50, p<0.01), with the highest value in Thanh Phu, Ben Tre (0.77±0.02 SE) and the lowest in Duyen Hai, Tra Vinh (0.70±0.02 SE), but this coefficient was not statistically significant between males (0.73±0.01 SE) and female (0.73±0.01 SE, t=0.64 p=0.52).
{"title":"Morphological Characteristics of Digestive Tract and Clark Index of Ellochelon vaigiensis (Quoy & Gaimard, 1825) in some Coastal Estuarine Areas in the Mekong Delta","authors":"Nguyen Thi Ngoc Anh, Nguyen Huu Duc Ton, Dinh Minh Quang","doi":"10.25073/2588-1140/vnunst.5456","DOIUrl":"https://doi.org/10.25073/2588-1140/vnunst.5456","url":null,"abstract":"Ellochelon vaigiensis (Quoy & Gaimard, 1825) distributes widely from marine to brackish and freshwater in the Indo-Pacific region, including Vietnam. In the Mekong Delta, this fish distributes mainly in the estuaries and is a commercial value fish species. However, information about this fish digestive tract morphology and energy storage capacity had been still unknown. These characteristics, after being elucidated, wil be the basis for further research on artificially rearing them, contributing to the diversity of cultured subjects. This study was carried out in several coastal areas of provinces in the Mekong Delta from Ben Tre to Tra Vinh, Soc Trang and Bac Lieu. The analysis results of a total of 656 samples (289 females and 367 males) collected monthly, from March to October 2021, showed that E. vaigiensis had a small mouth, thick gill comb, short stomach and long intestines. This showed that they belong to the group of herbivorous fish. Ellochelon vaigiensis displayed a high energy storage capacity due to its relatively large Clark coefficient (0.73±0.03 SE). This value in the wet season (0.66±0.01 SE) is lower than in the dry season (0.85±0.01 SE, t=16.98, p<0.01). This value changed significantly according to the study sites (1-way ANOVA, F=4.50, p<0.01), with the highest value in Thanh Phu, Ben Tre (0.77±0.02 SE) and the lowest in Duyen Hai, Tra Vinh (0.70±0.02 SE), but this coefficient was not statistically significant between males (0.73±0.01 SE) and female (0.73±0.01 SE, t=0.64 p=0.52).","PeriodicalId":23524,"journal":{"name":"VNU Journal of Science: Natural Sciences and Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80139560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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