Veterinary microbiology最新文献_第2页

STAT6 promotes innate immunity against BEFV and VSV by inhibiting STUB1 and NIX-mediated MAVS degradation STAT6 通过抑制 STUB1 和 NIX 介导的 MAVS 降解，促进对 BEFV 和 VSV 的先天免疫。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110290

Fuzhen Zhang , Hongmei Wang , Hongbin He , Peili Hou

Signal transducers and activators of transcription 6 (STAT6), an essential member of the STAT protein family, plays vital roles in innate immunity, however, its function in regulating innate immunity through the degradation of MAVS has not been described. In this study, we found that STAT6 suppresses the replication of both bovine ephemeral fever virus (BEFV) and vesicular stomatitis virus (VSV). Further investigations revealed that STAT6 promotes the type I IFN (IFN-I) signaling pathway in the context of BEFV and VSV infection. Moreover, the knockout of STAT6 leads to the degradation of MAVS through both the ubiquitin-proteasome and autophagolysosomal pathways. Mechanistically, STAT6 results in the downregulation of E3 ubiquitin ligase STIP1 homology and Ubox-containing protein 1 (STUB1), inhibits the interaction between STUB1 and MAVS, and reduces STUB1- mediated K48-linked MAVS ubiquitination, thereby inhibiting the MAVS degradation through the ubiquitin-proteasome pathway. Furthermore, STAT6 also suppresses MAVS degradation through the autophagy receptor Bcl2 interacting protein 3 like (NIX)-mediated autophagy pathway. Taken together, our study unveils a novel mechanism by which STAT6 acts as a positive regulator of the type I IFN signaling pathway during BEFV and VSV infection, predominantly by inhibiting MAVS degradation and ultimately suppressing BEFV and VSV infection. These findings provide valuable insights into the regulation of MAVS degradation by STAT6, which may serve as a basis for the design of novel antiviral agents.

信号转导子和转录激活子 6（STAT6）是 STAT 蛋白家族的重要成员，在先天性免疫中发挥着重要作用，但其通过降解 MAVS 来调节先天性免疫的功能尚未被描述。在这项研究中，我们发现 STAT6 可抑制牛短暂热病毒（BEFV）和水泡性口炎病毒（VSV）的复制。进一步的研究发现，STAT6 能在 BEFV 和 VSV 感染的情况下促进 I 型 IFN（IFN-I）信号通路。此外，敲除 STAT6 会导致 MAVS 通过泛素蛋白酶体和自噬溶酶体途径降解。从机理上讲，STAT6会导致E3泛素连接酶STIP1同源和含Ubox蛋白1（STUB1）的下调，抑制STUB1与MAVS之间的相互作用，减少STUB1介导的与K48连接的MAVS泛素化，从而抑制MAVS通过泛素-蛋白酶体途径降解。此外，STAT6 还能抑制 MAVS 通过自噬受体 Bcl2 交互蛋白 3 like（NIX）介导的自噬途径降解。综上所述，我们的研究揭示了一种新的机制，即 STAT6 在 BEFV 和 VSV 感染过程中作为 I 型 IFN 信号通路的正向调节因子，主要通过抑制 MAVS 降解并最终抑制 BEFV 和 VSV 感染。这些发现为 STAT6 对 MAVS 降解的调控提供了有价值的见解，可作为设计新型抗病毒药物的基础。

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引用次数: 0

The modulation of proteomics and antioxidant stress is involved in the effect of nitazoxanide against Japanese encephalitis virus in vitro 蛋白质组学和抗氧化压力的调节参与了硝唑沙尼对日本脑炎病毒的体外抗病毒作用

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110289

Yu Su , Yanping Wang , Chengeng Xiong , Xiaoyang Wang , Chunmei Wang , Wen Zhou , Donghai Zhou , Keyu Zhang

Japanese encephalitis virus (JEV) is a significant circulating arbovirus flavivirus and the primary cause of viral encephalitis in Asia. Previous studies have demonstrated that nitazoxanide (NTZ), an antiparasitic gastroenteritis medication classified as a thiazolide, exhibits efficacy against JEV both in vitro and in vivo. To explore the potential antiviral mechanisms, we employed Tandem Mass Tag (TMT)-based quantitative proteomics to identify differentially expressed proteins (DEPs) among three groups: Blank cell group, JEV-infected cell group, and JEV-infected cells treated with NTZ. Our analysis revealed that NTZ treatment led to the upregulation of 30 DEPs and downregulation of 54 DEPs in JEV-infected cells. Enrichment analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that these DEPs are involved in various biological processes and signaling pathways, including transport, localization, response to wounding, P53 pathway activation, and fatty acid metabolism-related pathways. Moreover, we observed that the expression trend of TMX2, a protein associated with redox homeostasis, was consistent with findings from TMT-based quantitative proteomics. Further investigations into reactive oxygen species (ROS), mitochondrial membrane potential, antioxidant enzyme activity, and the KEAP1-NRF2 pathway demonstrated that NTZ effectively regulates the KEAP1-NRF2 pathway while suppressing oxidative stress induced by JEV infection. In conclusion, the proteomic data along with antioxidant stress results presented herein provide a foundational basis for further research into the molecular mechanisms and potential targets of NTZ against JEV.

日本脑炎病毒（JEV）是一种重要的流行性虫媒黄病毒，也是亚洲病毒性脑炎的主要病因。以前的研究表明，硝唑沙尼（NTZ）是一种抗寄生虫胃肠炎药物，属于噻唑类药物，在体外和体内对日本脑炎病毒均有疗效。为了探索潜在的抗病毒机制，我们采用了基于串联质量标签（TMT）的定量蛋白质组学方法来鉴定三组之间的差异表达蛋白（DEPs）：空白细胞组、JEV 感染细胞组和经 NTZ 处理的 JEV 感染细胞组。我们的分析表明，NTZ 处理导致 JEV 感染细胞中 30 个 DEPs 上调，54 个 DEPs 下调。利用基因本体（GO）和京都基因组百科全书（KEGG）进行的富集分析表明，这些DEPs参与了多种生物过程和信号通路，包括转运、定位、对创伤的反应、P53通路激活和脂肪酸代谢相关通路。此外，我们还观察到与氧化还原稳态相关的蛋白质 TMX2 的表达趋势与基于 TMT 的定量蛋白质组学研究结果一致。对活性氧（ROS）、线粒体膜电位、抗氧化酶活性和 KEAP1-NRF2 通路的进一步研究表明，NTZ 能有效调节 KEAP1-NRF2 通路，同时抑制 JEV 感染诱导的氧化应激。总之，本文介绍的蛋白质组数据和抗氧化应激结果为进一步研究 NTZ 抗 JEV 的分子机制和潜在靶点提供了基础。

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引用次数: 0

Corrigendum to “ Development and characterization of monoclonal antibody against the critical loop structure of african swine fever virus P72 protein” [Vet. Microbiol. 283 (2023) 109776] 针对非洲猪瘟病毒 P72 蛋白关键环结构的单克隆抗体的开发和特征描述"[Vet. Microbiol.

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110272

Zejie Chang , Yongkun Du , Ruiqi Li , Xueke Sun , Yilan Chen , Minghui Li , Lu Fan , Siyuan Liu , Siqiao Wang , Peiyang Ding , Gaiping Zhang

引用次数: 0

A novel inactivated oral rabies vaccine with the incorporation of U-OMP19 enhances the immunogenicity by reducing viral proteins degradation and activating dendritic cells in a mouse model 加入 U-OMP19 的新型口服狂犬病灭活疫苗在小鼠模型中通过减少病毒蛋白降解和激活树突状细胞增强了免疫原性。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110287

Zonghui Zeng , Di Wu , Jingyi Xiong , Jianqing Zhao , Chuan Liang , Qiong Wu , Chengli Huang , Rui Zhou , Zhen F. Fu , Ling Zhao , Ming Zhou

Currently, dogs, especially stray dogs, and/or wild animals are the main sources of rabies transmission, and oral vaccination is the most practical way to control rabies in these animals. Safety and efficacy are two key criteria for developing oral vaccines. Concerning the efficacy of oral vaccines, degradation of immunogens by gastrointestinal fluid is a major challenge, resulting in suboptimal immune responses after vaccination. For safety reasons, inactivated vaccines are the most optimal choice. In the present study, a recombinant rabies virus (RABV) with un-lipidated outer membrane protein 19 (U-OMP19) of Brucella spp incorporated into RABV virions, designated as LBNSE-OMP19-G, was constructed and rescued. We found that U-OMP19 was incorporated into LBNSE-OMP19-G virion, which could protect RABV G protein from digestion by gastrointestinal fluids in vitro. Moreover, the immunogenicity of LBNSE-OMP19-G as an inactivated oral vaccine was evaluated, and the inactivated LBNSE-OMP19-G could activate more dendritic cells (DCs) and promote the generation of follicular helper T (T_FH) cells, germinal center (GC) B cells, and plasma cells in immunized mice compared with those in mice immunized with parent virus LNBSE, which consequently induced a higher level of virus neutralizing antibody and provided better protection after a lethal challenge of rabies. These data indicate that LBNSE-OMP19-G, which has good safety and immunogenicity, could be a potential inactivated oral rabies vaccine candidate.

目前，狗（尤其是流浪狗）和/或野生动物是狂犬病的主要传播源，而口服疫苗是控制这些动物感染狂犬病的最实用方法。安全性和有效性是开发口服疫苗的两个关键标准。关于口服疫苗的有效性，免疫原被胃肠液降解是一个主要挑战，导致疫苗接种后的免疫反应不理想。出于安全考虑，灭活疫苗是最佳选择。在本研究中，我们构建并挽救了一种重组狂犬病病毒（RABV），在 RABV 病毒中加入了布鲁氏菌的非脂质外膜蛋白 19（U-OMP19），命名为 LBNSE-OMP19-G。我们发现，将 U-OMP19 加入 LBNSE-OMP19-G 病毒中，可以保护 RABV G 蛋白不被体外胃肠液消化。此外，我们还评估了 LBNSE-OMP19-G 作为口服灭活疫苗的免疫原性，灭活的 LBNSE-OMP19-G 能激活更多的树突状细胞（DCs），促进滤泡辅助性 T 细胞（TFH）、生殖中心 B 细胞（GC）和浆细胞的生成、从而诱导更高水平的病毒中和抗体，并在狂犬病致死性挑战后提供更好的保护。这些数据表明，LBNSE-OMP19-G 具有良好的安全性和免疫原性，可作为一种潜在的口服狂犬病灭活疫苗候选物。

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引用次数: 0

Porcine reproductive and respiratory syndrome virus variant emergence: Example and considerations for prospective monitoring 猪繁殖与呼吸综合征病毒变种的出现：前瞻性监测的实例和考虑因素

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110293

Mariana Kikuti , Claudio Marcello Melini , Xiaomei Yue , Igor A.D. Paploski , Nakarin Pamornchainavakul , Julia P. Baker , Dennis N. Makau , Kimberly VanderWaal , Amy Maschhoff , Kayla Henness , Donna Drebes , Cesar A. Corzo

New PRRSV variants are constantly emerging due to the rapid evolution of this virus. We aimed to describe the emergence of a new PRRSV variant within sub-lineage 1 C, its space-time distribution, and its impact on affected herds. Additionally, we discuss considerations on how to monitor emerging PRRSV variants. This newly emerging variant was first detected in June 2022 on a sow herd undergoing a mild PRRS outbreak. Cases were defined by ORF5 nucleotide identity of ≥98 % between samples using the first detected case as a seed. A total of 382 case sequences were identified in sixteen production systems. Although most sequences originated from breeding sites (58.4 %) compared to grow-finishing sites (33.3 %), they corresponded to 118 individual sites (73 grow-finishing, 37 breeding, and 8 with no farm type information). Two spatial-temporal clusters in the Midwest were detected, but only when system was not accounted for. 63.6 % (21/33) of breeding herds reached stability in a median of 87 weeks (57 weeks in herds in which only the studied variant was detected, and 91 weeks when multiple PRRSV variants were involved). The average mortality in growing pig sites affected by this variant was not statistically different from the one found in L1C1–4–4 variant-affected sites. Altogether, these results pinpoint this as a variant of interest for continued surveillance due to increased time to stability than previously reported in the literature. Prospective monitoring of emerging variants should acknowledge the complex relationship between data limitations and multi-variant outbreaks, amongst other factors.

由于 PRRSV 的快速进化，该病毒不断出现新的变种。我们旨在描述 1 C 亚系中一种新的 PRRSV 变种的出现、其时空分布及其对受影响畜群的影响。此外，我们还讨论了如何监测新出现的 PRRSV 变种的注意事项。这种新出现的变异体于 2022 年 6 月在一个发生轻微 PRRS 疫情的母猪群中首次被检测到。以第一个检测到的病例为种子，通过样本间 ORF5 核苷酸同一性≥98%来定义病例。在 16 个生产系统中共鉴定出 382 个病例序列。虽然大多数序列来自育种基地（58.4%），而不是种植-精加工基地（33.3%），但它们对应于 118 个基地（73 个种植-精加工基地、37 个育种基地和 8 个无农场类型信息的基地）。在中西部发现了两个时空集群，但仅在不考虑系统的情况下。63.6%（21/33）的种猪群在中位数 87 周内达到稳定（仅检测到所研究变异体的种猪群为 57 周，涉及多个 PRRSV 变异体的种猪群为 91 周）。受该变异体影响的生长猪群的平均死亡率与受 L1C1-4-4 变异体影响的生长猪群的平均死亡率没有统计学差异。总之，这些结果表明，与以前的文献报道相比，该变异体的稳定时间更长，因此应继续对其进行监测。对新变异体的前瞻性监测应考虑到数据限制和多变异体爆发等因素之间的复杂关系。

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引用次数: 0

Retraction notice to “Andrographolide inhibits porcine epidemic diarrhea virus by inhibiting the JAK2-STAT3 pathway and promoting apoptosis” [Vet. Microbiol. 298 (2024) 110235] 穿心莲内酯通过抑制 JAK2-STAT3 通路和促进细胞凋亡抑制猪流行性腹泻病毒》[Vet. Microbiol.

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110300

Cong He , Rongjie Zhang , Liangyu Yang , Bin Xiang

引用次数: 0

Efficacy of a DNA vaccine encoding the E2 glycoprotein of bovine viral diarrhea virus 1 fused to mouse lysosome-associated membrane protein 1 编码与小鼠溶酶体相关膜蛋白 1 融合的牛病毒性腹泻病毒 1 的 E2 糖蛋白的 DNA 疫苗的功效。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110283

Yusuke Sakai , Shinji Yamada , Maho Inoue , Toshinori Shiga , Kotomi Konagayoshi , Kei Kasai , Atsushi Kimura , Kenji Murakami

The E2 protein of bovine viral diarrhea virus (BVDV) is a known protective antigen and a major target for DNA vaccines. DNA vaccines have various advantages; however, their immunogenicity needs to be enhanced by using adjuvants or drug delivery systems. In this study, we used mouse lysosome-associated membrane protein 1 (mLAMP1) as a molecular adjuvant and developed a DNA vaccine encoding an mLAMP1-BVDV E2 chimeric protein (pVax-mLAMP1-E2). We constructed DNA plasmids in which the E2 gene was inserted within the hinge region (H) or membrane proximal domain (D) of the mLAMP1 gene. Transfection of these plasmids into cultured cells led to high expression of E2 antigen from pVax-mLAMP1-E2 (H). Intradermal immunization of mice with pVax-mLAMP1-E2 (H) induced sufficient neutralizing antibodies and splenocytes with E2 antigen-specific IFN-γ production compared with pVax-mLAMP1-E2 (D). However, the immunogenicity of pVax mLAMP1-E2 (H) in mice did not differ from that of a control plasmid without the LAMP1 molecule (pVax-E2). In cattle, geometric mean serum neutralizing antibody titers after intradermal or intramuscular injection tended to be higher with pVax-mLAMP1-E2 (H) than with pVax that expressed E2 without mLAMP1. In addition, E2 antigen-specific IFN-γ production in peripheral blood mononuclear cells from cattle immunized intradermally with pVax-mLAMP1-E2 (H) was not significantly different from that of pVax-E2. These results suggest that mLAMP1 fusion antigens effectively induce humoral and cellular immunity in mice and cattle, especially when the antigen is inserted in the hinge region of mLAMP1. The LAMP1-E2 fusion antigen may be a useful candidate for a BVDV DNA vaccine in cattle.

牛病毒性腹泻病毒（BVDV）的 E2 蛋白是一种已知的保护性抗原，也是 DNA 疫苗的主要靶标。DNA 疫苗具有多种优势，但需要通过使用佐剂或给药系统来增强其免疫原性。在本研究中，我们使用小鼠溶酶体相关膜蛋白1（mLAMP1）作为分子佐剂，并开发了编码mLAMP1-BVDV E2嵌合蛋白的DNA疫苗（pVax-mLAMP1-E2）。我们构建了 DNA 质粒，将 E2 基因插入 mLAMP1 基因的铰链区（H）或膜近端结构域（D）。将这些质粒转染到培养细胞后，pVax-mLAMP1-E2（H）的E2抗原得到了高表达。与pVax-mLAMP1-E2（D）相比，用pVax-mLAMP1-E2（H）皮内免疫小鼠可诱导足够的中和抗体和脾细胞产生E2抗原特异性IFN-γ。然而，pVax mLAMP1-E2（H）在小鼠中的免疫原性与不含 LAMP1 分子的对照质粒（pVax-E2）没有区别。在牛身上，皮内或肌肉注射 pVax-mLAMP1-E2 (H) 后的几何平均血清中和抗体滴度往往高于表达 E2 而不表达 mLAMP1 的 pVax。此外，用 pVax-mLAMP1-E2 (H) 皮内免疫的牛的外周血单核细胞产生的 E2 抗原特异性 IFN-γ 与 pVax-E2 没有显著差异。这些结果表明，mLAMP1 融合抗原能有效诱导小鼠和牛的体液免疫和细胞免疫，尤其是当抗原插入 mLAMP1 的铰链区时。LAMP1-E2 融合抗原可能是牛 BVDV DNA 疫苗的有用候选抗原。

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引用次数: 0

Mycoplasma bovis activates apoptotic caspases to suppress xenophagy for its intracellular survival 牛支原体为在细胞内生存而激活凋亡树突酶抑制噬异性。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110298

Yinjuan Song , Li Tang , Na Li , Jian Xu , Zhengyang Zhang , Hui Ma , Yi Liao , Yuefeng Chu

Mammalian caspases are categorized into apoptotic and inflammatory types. Apoptotic caspases mediate apoptosis activation, while inflammatory caspases participate in inflammasome activation. Previous studies have shown that apoptotic caspases regulate autophagy in both cancer and pharmacological treatment models. However, the relationship between apoptotic caspases and xenophagy during pathogen infection remains elusive. In the current study, we used Mycoplasma bovis (M. bovis) as a model pathogen investigating the relationship between apoptotic caspases and xenophagy during infection. We found that M. bovis activated apoptotic caspases by triggering mitochondrial damage in macrophages, and the intracellular survival of M. bovis was enhanced by the activation of apoptotic caspases and restricted by the inhibition of apoptotic caspases. Moreover, confocal microscopy and Western blot analysis revealed that the activation of apoptotic caspases impedes host xenophagy by cleaving autophagy-related protein Beclin 1. Our findings indicate that M. bovis utilizes host apoptotic caspases to suppress xenophagy, thereby enhancing its intracellular survival. This research contributes to understanding the interplay between apoptotic caspases and xenophagy during pathogen infection, offering novel insights into the intracellular survival mechanisms of mycoplasma in macrophages.

哺乳动物的caspases分为凋亡型和炎症型。凋亡caspases介导细胞凋亡的激活，而炎症caspases则参与炎性体的激活。以往的研究表明，在癌症和药物治疗模型中，凋亡caspases都能调节自噬。然而，在病原体感染过程中，凋亡caspases与异种吞噬之间的关系仍然难以捉摸。在本研究中，我们以牛支原体（M. bovis）为模型病原体，研究了感染过程中凋亡caspases与吞噬异种细胞之间的关系。我们发现，牛支原体通过引发巨噬细胞线粒体损伤来激活凋亡caspases，凋亡caspases的激活增强了牛支原体在细胞内的存活，而凋亡caspases的抑制则限制了牛支原体在细胞内的存活。此外，共聚焦显微镜和 Western 印迹分析显示，凋亡caspases的激活通过裂解自噬相关蛋白Beclin 1来阻碍宿主的异噬作用。我们的研究结果表明，牛海绵状芽孢杆菌利用宿主的凋亡caspases来抑制异噬作用，从而提高其胞内存活率。这项研究有助于理解病原体感染过程中凋亡caspases和噬异性之间的相互作用，为了解支原体在巨噬细胞内的生存机制提供了新的视角。

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引用次数: 0

Genomic insights into qnrVC1 gene located on an IncP6 plasmid carried by multidrug resistant Pseudomonas aeruginosa from clinical asinine isolates 从临床阴性分离株中发现的具有多重耐药性的铜绿假单胞菌携带的 IncP6 质粒上的 qnrVC1 基因的基因组学启示

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110285

Yufei Zhao , Yiping Zhu , Weishuai Zhai , Luo Yang , Cong Peng , Junpeng Mi , Rongzheng Wu , Yuxin Xie , Dejun Liu , Jing Li

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen, causing significant global health threat due to its antimicrobial resistance. Among equines, P. aeruginosa can cause infections, particularly in the reproductive tract, leading to reproductive failure. Multidrug-resistant (MDR) P. aeruginosa has been a major concern in animal husbandry, including the donkey industry. The study aims to elucidate the phylogenetic relationship of P. aeruginosa strains isolated from donkeys with endometritis farmed in a large intensive unit in Hebei Province, China. Genes coding for multiple antimicrobial resistances were predicted by whole genomic sequencing. Multilocus sequence typing (MLST) revealed that all strains belonged to the same sequence type (ST1058). An IncP6 plasmid encoding the qnrVC1 gene, associated with quinolone resistance, was identified. Comparative genomic analysis illustrated the characteristics of the strains and genetic context of qnrVC1. This study is the first to report that these MDR P. aeruginosa asinine strains exhibited high levels of antimicrobial and metal resistance conferred by a qnrVC1-carrying plasmid. Additionally, P. aeruginosa strains with integrated mega-plasmids were identified. From a One Health perspective, the study underlined the significance of monitoring antimicrobial resistance genes in food animals, including donkeys.

铜绿假单胞菌（P. aeruginosa）是一种机会性病原体，由于其抗菌药耐药性而对全球健康造成严重威胁。在马匹中，铜绿假单胞菌可引起感染，尤其是生殖道感染，导致繁殖失败。耐多药（MDR）铜绿假单胞菌一直是包括驴业在内的畜牧业关注的焦点。本研究旨在阐明从中国河北省一个大型集约化养殖场中患有子宫内膜炎的驴身上分离出的铜绿假单胞菌株的系统发育关系。通过全基因组测序预测了编码多种抗菌素耐药性的基因。多焦点序列分型（MLST）显示，所有菌株都属于同一序列类型（ST1058）。鉴定出了编码 qnrVC1 基因的 IncP6 质粒，该质粒与喹诺酮类药物耐药性有关。基因组比较分析表明了菌株的特征和 qnrVC1 的遗传背景。本研究首次报道了这些 MDR 铜绿微囊桿菌asinine 菌株表现出了由携带 qnrVC1 的质粒赋予的高水平抗菌药和金属抗性。此外，还发现了整合了巨型质粒的铜绿假单胞菌菌株。从 "一体健康 "的角度来看，该研究强调了监测包括驴在内的食用动物抗菌药耐药性基因的重要性。

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引用次数: 0

A new subtype of serovar 6, K6b:O3, of Actinobacillus pleuropneumoniae based on genotypic analysis 基于基因型分析的胸膜肺炎放线杆菌 6 号血清 K6b:O3 新亚型。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology

Pub Date : 2024-11-01 DOI: 10.1016/j.vetmic.2024.110291

Ho To , Nobuyuki Tsutsumi , Michiha Kon , Nayu Kawashima , Fumiko Koike , Sonia Lacouture , Marcelo Gottschalk , Joachim Frey , Shinya Nagai

We have analyzed the capsule (CPS) and the lipopolysaccharide O-Antigen (O-Ag) biosynthesis loci of fifteen field isolates of Actinobacillus pleuropneumoniae, including eleven North American and four Japanese ones, reactive to antisera against serovars 3, 6, 8 and/or 15. Ten North American isolates amplified a serovar 6-indicative fragment derived from the capsular loci, whereas one North American isolate and all four Japanese isolates amplified the serovar 6-indicative fragment as well as the serovar 3-indicative fragment. The five isolates producing a 3/6 banding pattern contain a type I CPS locus, named K6b, similar to serovar 6, but with differences in the cpxABCD and cpsABC gene sequences and the length of intergenic regions (modF-cpxA, and cpsC-cpsD). The main difference found between the K6 and K6b cps genes is a loss of function of a 113 AA UDP-glycosyltransferase found in type 6b due to the amino acid substitutions in the C-terminal domain of Cps6bA. Additionally, the isolates harbor a LPS O-Ag locus highly identical to those of field and reference strains of serovars 3, 8, 15, 17 and 19 but different from that of serovar 6. Taken together, our results indicate the existence of a subtype of A. pleuropneumoniae, serovar 6, that we called “K6b:O3′′, and we propose isolate EH1248 as the reference strain.

我们分析了 15 个胸膜肺炎放线杆菌野外分离株（包括 11 个北美分离株和 4 个日本分离株）的胶囊（CPS）和脂多糖 O 抗原（O-Ag）生物合成位点，这些分离株对血清 3、6、8 和/或 15 抗血清反应。十个北美分离物扩增了来自荚膜位点的血清 6-指示性片段，而一个北美分离物和所有四个日本分离物扩增了血清 6-指示性片段以及血清 3-指示性片段。产生 3/6 带状模式的五个分离株含有一个 I 型 CPS 基因座，命名为 K6b，与血清 6 类似，但 cpxABCD 和 cpsABC 基因序列以及基因间区（modF-cpxA 和 cpsC-cpsD）的长度不同。K6 和 K6b cps 基因的主要区别在于，由于 Cps6bA C 端结构域的氨基酸替换，6b 型中的 113 AA UDP-糖基转移酶失去了功能。此外，这些分离株的 LPS O-Ag 基因座与 3、8、15、17 和 19 型血清的野外菌株和参考菌株的基因座高度一致，但与 6 型血清的基因座不同。综上所述，我们的研究结果表明胸膜肺炎甲菌存在一个亚型，即血清 6 型，我们称之为 "K6b:O3''，并建议以分离株 EH1248 作为参考菌株。

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引用次数: 0