Pub Date : 2023-06-07DOI: 10.15625/2525-2518/18064
Do Thuy Chi, Nguyen Thuy Van, Vu Duc Chinh, Hoang Thi Hong Cam, Vilaysak Sayyasone, Pham Thanh Binh, Bui Huy, Pham Van Hoi
Porous silicon photonic crystal (PSi PhC) decorated with silver nanoparticles (AgNPs) is shown to provide a new substrate for the improvement of SERS. AgNPs are obtained through immersion of PSi PhC samples in AgNO3 solutions and successive thermal annealing. The nanocomposite material generated by adding AgNPs inside the silicon nanoscale pores, integrates the ability of metal surfaces to amplify Raman scattering signals and an enlarged surface area. Besides, PhC structure formed by multilayer PSi increases interaction time of light and matter of the multilayer structure. The experimental results show that the AgNPs-decorated PSi PhC has the largest Raman intensity in comparison with other SERS substrates based on Si. The enhancement of Raman signals also reduces the threshold of the detection down to below 10-10 M. The analytical enhancement factor of AgNPs/PSi PhC SERS substrate for the detection of Rhodamine B (RhB) reaches 1010. This proposed AgNPs/PSi PhC SERS substrate could serve as a potential candidate for detecting RhB in foodstuffs at very low concentrations.
{"title":"Efficient and reliable detection of rhodamine B using SERS from silver-decorated photonic crystal silicon nanoscale pores","authors":"Do Thuy Chi, Nguyen Thuy Van, Vu Duc Chinh, Hoang Thi Hong Cam, Vilaysak Sayyasone, Pham Thanh Binh, Bui Huy, Pham Van Hoi","doi":"10.15625/2525-2518/18064","DOIUrl":"https://doi.org/10.15625/2525-2518/18064","url":null,"abstract":"Porous silicon photonic crystal (PSi PhC) decorated with silver nanoparticles (AgNPs) is shown to provide a new substrate for the improvement of SERS. AgNPs are obtained through immersion of PSi PhC samples in AgNO3 solutions and successive thermal annealing. The nanocomposite material generated by adding AgNPs inside the silicon nanoscale pores, integrates the ability of metal surfaces to amplify Raman scattering signals and an enlarged surface area. Besides, PhC structure formed by multilayer PSi increases interaction time of light and matter of the multilayer structure. The experimental results show that the AgNPs-decorated PSi PhC has the largest Raman intensity in comparison with other SERS substrates based on Si. The enhancement of Raman signals also reduces the threshold of the detection down to below 10-10 M. The analytical enhancement factor of AgNPs/PSi PhC SERS substrate for the detection of Rhodamine B (RhB) reaches 1010. This proposed AgNPs/PSi PhC SERS substrate could serve as a potential candidate for detecting RhB in foodstuffs at very low concentrations.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74375210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-07DOI: 10.15625/2525-2518/17250
T. Pham, T. Nguyen, K. To
In this study, the culture medium and fermentation modes were studied aiming to improve the cell density of Bacillus clausii. Firstly, the factorial design method using Minimum Run Resolution IV design was used to evaluate the relative importance of culture medium components to the growth of Bacillus clausii. The results showed that three components peptone, yeast extract and malt extract were the components significantly affecting the bacterial biomass. Then, the optimization of these three ingredient concentrations using a response surface methodology with the Box-Behnken design resulted that the maximal biomass had been achieved using the medium containing 7.64 g/L peptone, 10 g/L yeast extract and 6.36 g/L malt extract. Finally, the pH-stat fed batch fermentation was conducted in a 2-liter bioreactor where the 9X concentrated optimal culture medium was feed into the bioreactor based on the pH signal. As a result, the microbial cell density increased by 2.9-fold compared to that achieved through batch fermentation.
{"title":"Improvement of Bacillus clausii cell HH1 density via culture medium optimization and pH-stat fed batch fermentation","authors":"T. Pham, T. Nguyen, K. To","doi":"10.15625/2525-2518/17250","DOIUrl":"https://doi.org/10.15625/2525-2518/17250","url":null,"abstract":"\u0000In this study, the culture medium and fermentation modes were studied aiming to improve the cell density of Bacillus clausii. Firstly, the factorial design method using Minimum Run Resolution IV design was used to evaluate the relative importance of culture medium components to the growth of Bacillus clausii. The results showed that three components peptone, yeast extract and malt extract were the components significantly affecting the bacterial biomass. Then, the optimization of these three ingredient concentrations using a response surface methodology with the Box-Behnken design resulted that the maximal biomass had been achieved using the medium containing 7.64 g/L peptone, 10 g/L yeast extract and 6.36 g/L malt extract. Finally, the pH-stat fed batch fermentation was conducted in a 2-liter bioreactor where the 9X concentrated optimal culture medium was feed into the bioreactor based on the pH signal. As a result, the microbial cell density increased by 2.9-fold compared to that achieved through batch fermentation.\u0000","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79196283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-05DOI: 10.15625/2525-2518/17249
Nguyễn Chính Nghĩa, Thi Phuong Duyen Vu, S. Chu-Ky, Hong Son Vu
The application of raw-starch hydrolysis enzymes to ethanol production by no-cook technology at very high gravity (VHG) (311.5 g/L) from rice would reduce the energy consumption compared to traditional process. In this study, the Taguchi design and the desirability function were used to identify the optimized production process for economic and technological targets. Three factors: dosage of alpha- and gluco-amylase enzyme (Stargen 002 – A), dosage of protease enzyme (Fermgen – B) and dosage of yeast (C) were investigated. With the expected Desirability = 0.74, the no-cook process from rice would be optimized with following dosage: Stargen 002, Fermgen and yeast at 700 µL/L (1140 GAU/kg raw material), 151 µL/L (431 SAPU/kg raw material) and 0.25 g/L (6.25 x 106 cells/mL), respectively. After 120h of fermentation, ethanol concentration reached 17.45 ± 0.07 %v/v, corresponding to ethanol yield of 86.08 ± 0.2 %, and the cost of raw material was at 3225.5 VND/L of rice slurry.
{"title":"OPTIMIZATION OF NO COOK TECHNOLOGY AT VERY HIGH GRAVITY FOR RICE-BASED ETHANOL PRODUCTION","authors":"Nguyễn Chính Nghĩa, Thi Phuong Duyen Vu, S. Chu-Ky, Hong Son Vu","doi":"10.15625/2525-2518/17249","DOIUrl":"https://doi.org/10.15625/2525-2518/17249","url":null,"abstract":"The application of raw-starch hydrolysis enzymes to ethanol production by no-cook technology at very high gravity (VHG) (311.5 g/L) from rice would reduce the energy consumption compared to traditional process. In this study, the Taguchi design and the desirability function were used to identify the optimized production process for economic and technological targets. Three factors: dosage of alpha- and gluco-amylase enzyme (Stargen 002 – A), dosage of protease enzyme (Fermgen – B) and dosage of yeast (C) were investigated. With the expected Desirability = 0.74, the no-cook process from rice would be optimized with following dosage: Stargen 002, Fermgen and yeast at 700 µL/L (1140 GAU/kg raw material), 151 µL/L (431 SAPU/kg raw material) and 0.25 g/L (6.25 x 106 cells/mL), respectively. After 120h of fermentation, ethanol concentration reached 17.45 ± 0.07 %v/v, corresponding to ethanol yield of 86.08 ± 0.2 %, and the cost of raw material was at 3225.5 VND/L of rice slurry.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74165333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-26DOI: 10.15625/2525-2518/17372
Nguyen Duc Hung, Tran Van Cong, Do Le Thanh Hung, Tran Thi Ngoc Dung
Electrochemical plasma is formed by an electrochemical reaction with a high voltage direct current (DC) in a non-electrolyte solution such as distilled water to create a gaseous state on the electrodes. Under conditions of high voltage, strong electric field and suitable temperature rise due to the Joule-Lenz effect, an electrochemical plasma will appear and be noted by the glow on the electrode as well as the characteristic amperage of the reaction rate leading to a rapid increase in the electrochemical reaction. Plasma reactions produce substances with strong chemical reactivity such as H2, H2O2, or OH* free radicals, etc. in the aquatic environment. Electrochemical plasma formation can be controlled by technological parameters such as voltage, the distance between electrodes, conductivity, and the metallic nature of electrodes, etc. Using electrochemical plasma, it is possible to treat pollutants harmful to the aquatic environment such as 2,4-D; 2,4,5-T, and ammonia. In the process of treating polluted water by electrochemical plasma technology, chemicals and treatment materials are not used, so it is an environmentally friendly technology.
{"title":"Environmentally friendly technology for treatment of pesticide and ammonia contaminated water with electrochemical plasma","authors":"Nguyen Duc Hung, Tran Van Cong, Do Le Thanh Hung, Tran Thi Ngoc Dung","doi":"10.15625/2525-2518/17372","DOIUrl":"https://doi.org/10.15625/2525-2518/17372","url":null,"abstract":"Electrochemical plasma is formed by an electrochemical reaction with a high voltage direct current (DC) in a non-electrolyte solution such as distilled water to create a gaseous state on the electrodes. Under conditions of high voltage, strong electric field and suitable temperature rise due to the Joule-Lenz effect, an electrochemical plasma will appear and be noted by the glow on the electrode as well as the characteristic amperage of the reaction rate leading to a rapid increase in the electrochemical reaction. Plasma reactions produce substances with strong chemical reactivity such as H2, H2O2, or OH* free radicals, etc. in the aquatic environment. Electrochemical plasma formation can be controlled by technological parameters such as voltage, the distance between electrodes, conductivity, and the metallic nature of electrodes, etc.\u0000Using electrochemical plasma, it is possible to treat pollutants harmful to the aquatic environment such as 2,4-D; 2,4,5-T, and ammonia. In the process of treating polluted water by electrochemical plasma technology, chemicals and treatment materials are not used, so it is an environmentally friendly technology.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72817605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-26DOI: 10.15625/2525-2518/17167
Le Thi Huyen, Nguyen Tien Dung
Ixeris dentata (Thunb. ex Thunb.) Nakai (family Asteraceae) a plant widely distributed in Viet Nam, is a perennial herb that grows to a height of 0.5 m. It has been used as a medicinal herb medicine since ancient times for the treatment of calculi, dyspepsia, indigestion, pneumonia, hepatitis, contusion, and tumors in Northeast Asia. The biological activities of this plant have been shown to be neuroprotective, anti-mutagenic, anti-hyperlipidemic, anti-inflammatory, anti-allergic, and anti-proliferative activities. It is also composed of aliphatics, triterpenoids, sesquiterpene, and glycosides. However, research on the phytochemistry of this plant has yet to be conducted in Viet Nam. In this study, five phenolics including chlorogenic acid (1), 3,5-di-O-caffeoylquinic acid (2), 4,5-di-O-caffeoylquinic acid (3), citrusin C (4), 4-allyl-2,6-dimethoxyphenol O-β-D-glupyranoside (5), and two sesquiterpene lactones, 8-epidesacylcynaropicrin glucoside (6) and ixerisoside A (7) were isolated from the methanol extract of the leaves of Ixeris dentata using combined chromatographic methods. Their chemical structures were determined by analysis of MS, NMR spectra data as well as comparison with those reported in the literature.
刺齿蛇(拇指)例研究)。Nakai(菊科)一种广泛分布于越南的植物，是一种多年生草本植物，生长高度为0.5米。自古以来，它就被用作一种草药，用于治疗肾结石、消化不良、消化不良、肺炎、肝炎、挫伤和肿瘤。该植物的生物活性已被证明具有神经保护、抗诱变、抗高脂血症、抗炎、抗过敏和抗增殖活性。它也由脂肪族、三萜、倍半萜和糖苷组成。然而，关于这种植物的植物化学研究尚未在越南进行。本研究采用联合色谱法从雪芹叶甲醇提取物中分离得到绿原酸(1)、3,5-二-O-咖啡酰基奎宁酸(2)、4,5-二-O-咖啡酰基奎宁酸(3)、柑橘素C(4)、4-烯丙基-2,6-二甲氧基苯酚O-β- d -葡糖苷(5)和两种倍半萜内酯8-epidesacylcynaropicrin gluco苷(6)和雪芹苷A(7)。通过质谱、核磁共振谱数据分析并与文献报道的化学结构进行比较，确定了它们的化学结构。
{"title":"Study on chemical constituents from Ixeris dentata","authors":"Le Thi Huyen, Nguyen Tien Dung","doi":"10.15625/2525-2518/17167","DOIUrl":"https://doi.org/10.15625/2525-2518/17167","url":null,"abstract":"Ixeris dentata (Thunb. ex Thunb.) Nakai (family Asteraceae) a plant widely distributed in Viet Nam, is a perennial herb that grows to a height of 0.5 m. It has been used as a medicinal herb medicine since ancient times for the treatment of calculi, dyspepsia, indigestion, pneumonia, hepatitis, contusion, and tumors in Northeast Asia. The biological activities of this plant have been shown to be neuroprotective, anti-mutagenic, anti-hyperlipidemic, anti-inflammatory, anti-allergic, and anti-proliferative activities. It is also composed of aliphatics, triterpenoids, sesquiterpene, and glycosides. However, research on the phytochemistry of this plant has yet to be conducted in Viet Nam. In this study, five phenolics including chlorogenic acid (1), 3,5-di-O-caffeoylquinic acid (2), 4,5-di-O-caffeoylquinic acid (3), citrusin C (4), 4-allyl-2,6-dimethoxyphenol O-β-D-glupyranoside (5), and two sesquiterpene lactones, 8-epidesacylcynaropicrin glucoside (6) and ixerisoside A (7) were isolated from the methanol extract of the leaves of Ixeris dentata using combined chromatographic methods. Their chemical structures were determined by analysis of MS, NMR spectra data as well as comparison with those reported in the literature.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89914223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, four common bivalves (including Meretrix lusoria (clam), Anadara subcrenata (ark clam), Crassostrea gigas (oyster), and Perna viridi (green mussels)), which are more popularly consumed were collected from a fishery market in Phu Yen, Central Vietnam to investigate the occurrence of microplastics (MPs) in their tissues. Samples weretreated with a KOH solution of 10 % for 48 hours and incubated at 40 °C. The base-sample ratio was 10:1 v:w (i.e 10 mL of 10 % KOH solution per 1.0 gram of a tissue sample). The treatment followed by sodium iodide-based extraction was set as the protocol for the separation of MPs from biological materials. The number of MP items determined in clam, ark clam, oyster, and green mussels registered by weight and individual were 0.3 ± 0.1, 0.2 ± 0.2, 0.3 ± 0.1, and 0.5 ± 0.3(item/g-ww), and 1,4 ± 0.8, 1.5 ± 1.4, 3.0 ± 2.4, 2.3 ± 1.2 (items/individual), respectively. Fiber is the common shape (69-92% of total items), followed by fragment. Furthermore, Polyethylene terephthalate was confirmed as the polymer of the common fiber MPs found in collected samples. As the first investigation on MPs in bivalve tissue from Phu Yen, Central Vietnam, this study indicated that a relatively high level of MPs was found in the commercial bivalves collected from fishery market of Central Vietnam, warning a potentially high risk of human consuming bivalves as daily food is posed. Keywords: microplastics, bivalves, Phu Yen, Central Vietnam. Classification numbers: 3.2.1, 3.6.1.
{"title":"Preliminary determination of microplastics in bivalves collected from Phu Yen, central Viet Nam","authors":"Tran Thi Ai My, Nguyen Duy Dat, Nguyen Quoc Hung, Duong Tuan Quang","doi":"10.15625/2525-2518/17032","DOIUrl":"https://doi.org/10.15625/2525-2518/17032","url":null,"abstract":"In this study, four common bivalves (including Meretrix lusoria (clam), Anadara subcrenata (ark clam), Crassostrea gigas (oyster), and Perna viridi (green mussels)), which are more popularly consumed were collected from a fishery market in Phu Yen, Central Vietnam to investigate the occurrence of microplastics (MPs) in their tissues. Samples weretreated with a KOH solution of 10 % for 48 hours and incubated at 40 °C. The base-sample ratio was 10:1 v:w (i.e 10 mL of 10 % KOH solution per 1.0 gram of a tissue sample). The treatment followed by sodium iodide-based extraction was set as the protocol for the separation of MPs from biological materials. The number of MP items determined in clam, ark clam, oyster, and green mussels registered by weight and individual were 0.3 ± 0.1, 0.2 ± 0.2, 0.3 ± 0.1, and 0.5 ± 0.3(item/g-ww), and 1,4 ± 0.8, 1.5 ± 1.4, 3.0 ± 2.4, 2.3 ± 1.2 (items/individual), respectively. Fiber is the common shape (69-92% of total items), followed by fragment. Furthermore, Polyethylene terephthalate was confirmed as the polymer of the common fiber MPs found in collected samples. As the first investigation on MPs in bivalve tissue from Phu Yen, Central Vietnam, this study indicated that a relatively high level of MPs was found in the commercial bivalves collected from fishery market of Central Vietnam, warning a potentially high risk of human consuming bivalves as daily food is posed. \u0000Keywords: microplastics, bivalves, Phu Yen, Central Vietnam. \u0000Classification numbers: 3.2.1, 3.6.1.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"221 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76637304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-18DOI: 10.15625/2525-2518/18040
V. Nguyen, San-Lang Wang, T. Nguyen, A. Nguyen
Black pepper is one of the most important industrial crops with high economic value for export. This crop is planted in Vietnam, Indonesia, India, and Brazil. Vietnam is the largest producer and exporter of peppercorns. This crop is widely cultivated in the Central Highlands and southeastern areas, with about 124,500 hectares, producing the largest amount of black peppercorns at 193,300 tons per year. However, the cultivation of this plant has faced various pathogen diseases, including the root–knot nematodes (RKNs). For years, many methods have been investigated for managing this disease. Especially in recent years, the number of studies on screening and utilization of beneficial microbes and their active secondary metabolites for effective treatment of RKNs has increased and is also recognized as an emerging research topic. In this review, we discuss and emphasize the significant research results concerning this novel strategy for the effective management of black pepper root-knot nematodes, as well as future studies in this direction. This review provides complete scientific information which may be useful for further investigation of sustainable cultivation of black pepper crops. Keywords: Black pepper, root–knot nematodes, beneficial microbes, nematocidal compounds, microbial fermentation.
{"title":"Emerging strategy for eco-friendly management of black pepper root-knot nematodes","authors":"V. Nguyen, San-Lang Wang, T. Nguyen, A. Nguyen","doi":"10.15625/2525-2518/18040","DOIUrl":"https://doi.org/10.15625/2525-2518/18040","url":null,"abstract":"Black pepper is one of the most important industrial crops with high economic value for export. This crop is planted in Vietnam, Indonesia, India, and Brazil. Vietnam is the largest producer and exporter of peppercorns. This crop is widely cultivated in the Central Highlands and southeastern areas, with about 124,500 hectares, producing the largest amount of black peppercorns at 193,300 tons per year. However, the cultivation of this plant has faced various pathogen diseases, including the root–knot nematodes (RKNs). For years, many methods have been investigated for managing this disease. Especially in recent years, the number of studies on screening and utilization of beneficial microbes and their active secondary metabolites for effective treatment of RKNs has increased and is also recognized as an emerging research topic. In this review, we discuss and emphasize the significant research results concerning this novel strategy for the effective management of black pepper root-knot nematodes, as well as future studies in this direction. This review provides complete scientific information which may be useful for further investigation of sustainable cultivation of black pepper crops. \u0000Keywords: Black pepper, root–knot nematodes, beneficial microbes, nematocidal compounds, microbial fermentation.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83679394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-18DOI: 10.15625/2525-2518/17218
C. Le, Vien Do Thi Hoa, Ha Ho Phu
Phytoestrogens, structurally and functionally of mammalian estrogens, are phenolic soybean-derived compounds. The aglycone phytoestrogens in soy germ including daidzein, genistein, and glycitein have been known for higher biological activity than the other derivative forms. The objective of this study was to assess the potential for pure β-glucosidase to hydrolyze the glucoside isoflavones in phytoestrogens extract from the soy germ to enrich the aglycones content of the extract. The optimum condition for the enrichment of aglycones from soy germ evaporated extract is β-glucosidase at 4 units/g of defatted soy germ flour, and an incubation time of 5.5 hours. Under this condition, the hydrolysis of β-glycosides, malonyl glycosides, and acetyl glycosides, were more than 90%, 60%, and 60%, respectively. The isoflavone aglycones content was 31.36 ± 0.06 µmol/g (14.97 ± 0.03 µmol/g daidzein, 7.58 ± 0.05 µmol glycitein, 8.81 ± 0.02 µmol/g genistein) was 5 fold more than that of defatted soy germ flour.
{"title":"Enrichment aglycone phytoestrogens of the extract from soy germ: A comprehensive study","authors":"C. Le, Vien Do Thi Hoa, Ha Ho Phu","doi":"10.15625/2525-2518/17218","DOIUrl":"https://doi.org/10.15625/2525-2518/17218","url":null,"abstract":"Phytoestrogens, structurally and functionally of mammalian estrogens, are phenolic soybean-derived compounds. The aglycone phytoestrogens in soy germ including daidzein, genistein, and glycitein have been known for higher biological activity than the other derivative forms. The objective of this study was to assess the potential for pure β-glucosidase to hydrolyze the glucoside isoflavones in phytoestrogens extract from the soy germ to enrich the aglycones content of the extract. The optimum condition for the enrichment of aglycones from soy germ evaporated extract is β-glucosidase at 4 units/g of defatted soy germ flour, and an incubation time of 5.5 hours. Under this condition, the hydrolysis of β-glycosides, malonyl glycosides, and acetyl glycosides, were more than 90%, 60%, and 60%, respectively. The isoflavone aglycones content was 31.36 ± 0.06 µmol/g (14.97 ± 0.03 µmol/g daidzein, 7.58 ± 0.05 µmol glycitein, 8.81 ± 0.02 µmol/g genistein) was 5 fold more than that of defatted soy germ flour.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"239 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76882170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.15625/2525-2518/17571
Le Nguyen Thanh, Tran Huu Giap, Vu Thi Hue, Diep Thi Lan Phuong, Nguyen Thi Hue, Nguyen Hoang Nam, Nguyen Quoc Vuong
Knema (Myristicaceae family) is a large genus, comprising over 100 species found in of South East Asian countries, among that over 10 species was found in Vietnam. Knema pachycarpa is a native tree distributed in central provinces of Viet Nam. Until now, only few chemical studies of Knema species in Viet Nam have been reported. Phytochemical study of Knema pachycarpa leaves led to the isolation of seven known compounds including six flavonoids 5,7,3′-trihydroxy-5′-methoxy isoflavone (1), luteolin (2), (-)-narigenin (3), (+)-catechin (4), isoquercetrin (5), and nicotiflorin (6), and a biphenyl compound 3,3',4,4'-tetrahydroxybiphenyl (7). Their structures were elucidated by MS and NMR data analysis and compared with those previously reported. Compounds 3 and 6-7 were found from Knema genus for the first time. In the cytotoxic assay, biphenyl compound (7) exhibited strong cytotoxicity to KB cancer cell line with IC50 value of 2.38 ± 0.11 mg/mL. Compound 7 also displayed showed activity against HepG2, Lu and MCF7 cancer cell lines with IC50 ranges from 18.25 ± 0.92 mg/mL to 35.56 ± 1.17 mg/mL, whereas flavonoids 1-6 were weakly active or inactive against tested cell lines.
{"title":"Phenolic compounds from knema pachycarpa leaves","authors":"Le Nguyen Thanh, Tran Huu Giap, Vu Thi Hue, Diep Thi Lan Phuong, Nguyen Thi Hue, Nguyen Hoang Nam, Nguyen Quoc Vuong","doi":"10.15625/2525-2518/17571","DOIUrl":"https://doi.org/10.15625/2525-2518/17571","url":null,"abstract":"Knema (Myristicaceae family) is a large genus, comprising over 100 species found in of South East Asian countries, among that over 10 species was found in Vietnam. Knema pachycarpa is a native tree distributed in central provinces of Viet Nam. Until now, only few chemical studies of Knema species in Viet Nam have been reported. Phytochemical study of Knema pachycarpa leaves led to the isolation of seven known compounds including six flavonoids 5,7,3′-trihydroxy-5′-methoxy isoflavone (1), luteolin (2), (-)-narigenin (3), (+)-catechin (4), isoquercetrin (5), and nicotiflorin (6), and a biphenyl compound 3,3',4,4'-tetrahydroxybiphenyl (7). Their structures were elucidated by MS and NMR data analysis and compared with those previously reported. Compounds 3 and 6-7 were found from Knema genus for the first time. In the cytotoxic assay, biphenyl compound (7) exhibited strong cytotoxicity to KB cancer cell line with IC50 value of 2.38 ± 0.11 mg/mL. Compound 7 also displayed showed activity against HepG2, Lu and MCF7 cancer cell lines with IC50 ranges from 18.25 ± 0.92 mg/mL to 35.56 ± 1.17 mg/mL, whereas flavonoids 1-6 were weakly active or inactive against tested cell lines.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84191994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.15625/2525-2518/17620
Le Dang Quang, Nguyen Tuan Anh, Tran Quang De, Nguyen Thi Thu Trang, Vu Xuan Minh, Nguyen Cuong Quoc, Vo T. Kieu Anh, Bui Van Cuong, Tran Dai Lam, Tran Thanh Men, Do Tan Khang, Nguyen Trong Tuan
Colletotrichum species cause anthracnose in various tropical crops in both cultivation and postharvest periods. The current synthetic fungicides to treat anthracnose sometime show toxic effects for farmers, residues on foods, and environmental impacts. In this work, turmeric extract containing curcuminoids was used as an active ingredient and formulated into a nanoemulsion to control anthracnose in red pepper and tomato fruits. The nanoparticles containing turmeric extract (25 %) were successfully prepared by encapsulation using Tween-80 and PEG 400 as emulsifiers. Out of the three main curcuminoids in the turmeric extract, BDMC was determined as the most abundant constituent via HPLC analysis. Turmeric nanoparticles showed an average particle size of 203 nm, polydispersity index (PI) of 0.322, and zeta potential of -92.7 mV. In the in vitro antifungal bioassay, this nanoformulation significantly inhibited the mycelial growth of Colletotrichum gloeosporioides and Colletotrichum orbiculare in a dose-dependent manner. Red pepper anthracnose was also consistently suppressed by turmeric nanoparticles but the tomato anthracnose was resistant to this formulation at 10 mg/mL in vivo. The study results proved the effectiveness of turmeric nanoparticles to control red pepper anthracnose in postharvest preservation and suggested developing the innovative nanoformulation as a green fungicide.
{"title":"Preparation and potential of nanoparticles containing curcuminoids to control fungal diseases in tropical fruits","authors":"Le Dang Quang, Nguyen Tuan Anh, Tran Quang De, Nguyen Thi Thu Trang, Vu Xuan Minh, Nguyen Cuong Quoc, Vo T. Kieu Anh, Bui Van Cuong, Tran Dai Lam, Tran Thanh Men, Do Tan Khang, Nguyen Trong Tuan","doi":"10.15625/2525-2518/17620","DOIUrl":"https://doi.org/10.15625/2525-2518/17620","url":null,"abstract":"Colletotrichum species cause anthracnose in various tropical crops in both cultivation and postharvest periods. The current synthetic fungicides to treat anthracnose sometime show toxic effects for farmers, residues on foods, and environmental impacts. In this work, turmeric extract containing curcuminoids was used as an active ingredient and formulated into a nanoemulsion to control anthracnose in red pepper and tomato fruits. The nanoparticles containing turmeric extract (25 %) were successfully prepared by encapsulation using Tween-80 and PEG 400 as emulsifiers. Out of the three main curcuminoids in the turmeric extract, BDMC was determined as the most abundant constituent via HPLC analysis. Turmeric nanoparticles showed an average particle size of 203 nm, polydispersity index (PI) of 0.322, and zeta potential of -92.7 mV. In the in vitro antifungal bioassay, this nanoformulation significantly inhibited the mycelial growth of Colletotrichum gloeosporioides and Colletotrichum orbiculare in a dose-dependent manner. Red pepper anthracnose was also consistently suppressed by turmeric nanoparticles but the tomato anthracnose was resistant to this formulation at 10 mg/mL in vivo. The study results proved the effectiveness of turmeric nanoparticles to control red pepper anthracnose in postharvest preservation and suggested developing the innovative nanoformulation as a green fungicide.","PeriodicalId":23553,"journal":{"name":"Vietnam Journal of Science and Technology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84579705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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