Pub Date : 2020-04-07DOI: 10.5073/VITIS.2020.59.53-61
J. Pouzoulet, A. Pivovaroff, E. Scudiero, M. E. D. Guzman, P. Rolshausen, L. Santiago
Xylem hydraulic properties of agricultural crop species can be linked to their region of origin, but because crop systems are often irrigated to reach optimum quality and yield, key differences in drought resistance are not often considered. We investigated how hydraulic conductivity and xylem vulnerability to drought-induced cavitation of two grapevine cultivars correspond to their centers of domestication with 'Merlot' (Vitis vinifera subspecies occidentalis) having been domesticated in a temperate forest region, and 'Thompson Seedless' (Vitis vinifera subspecies orientalis) domesticated in a semi-arid region. We used anatomical measurements and xylem vulnerability curves to evaluate hydraulic traits and drought resistance. Our results showed that 'Thompson Seedless' was significantly more vulnerable to drought-induced cavitation than 'Merlot'. Bench dehydration produced significantly different estimations of xylem vulnerability to cavitation in each cultivar. This result was consistent with anatomical measurement, with 'Thompson Seedless' stems having greater maximum stem-specific hydraulic conductivity, more vessels, higher vessel density and a greater lumen fraction than 'Merlot'. The relatively large amount of xylem vessels and lumen area in 'Thompson Seedless' is consistent with domestication in a semi-arid habitat where a greater number and size diversity of xylem vessels would be needed to transport water and meet evaporative demand as opposed to cultivars that were domesticated in temperate forest regions like 'Merlot'. These differences appear to expose 'Thompson Seedless' to high xylem vulnerability to cavitation.
{"title":"Contrasting adaptation of xylem to dehydration in two Vitis vinifera L. sub-species","authors":"J. Pouzoulet, A. Pivovaroff, E. Scudiero, M. E. D. Guzman, P. Rolshausen, L. Santiago","doi":"10.5073/VITIS.2020.59.53-61","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.53-61","url":null,"abstract":"Xylem hydraulic properties of agricultural crop species can be linked to their region of origin, but because crop systems are often irrigated to reach optimum quality and yield, key differences in drought resistance are not often considered. We investigated how hydraulic conductivity and xylem vulnerability to drought-induced cavitation of two grapevine cultivars correspond to their centers of domestication with 'Merlot' (Vitis vinifera subspecies occidentalis) having been domesticated in a temperate forest region, and 'Thompson Seedless' (Vitis vinifera subspecies orientalis) domesticated in a semi-arid region. We used anatomical measurements and xylem vulnerability curves to evaluate hydraulic traits and drought resistance. Our results showed that 'Thompson Seedless' was significantly more vulnerable to drought-induced cavitation than 'Merlot'. Bench dehydration produced significantly different estimations of xylem vulnerability to cavitation in each cultivar. This result was consistent with anatomical measurement, with 'Thompson Seedless' stems having greater maximum stem-specific hydraulic conductivity, more vessels, higher vessel density and a greater lumen fraction than 'Merlot'. The relatively large amount of xylem vessels and lumen area in 'Thompson Seedless' is consistent with domestication in a semi-arid habitat where a greater number and size diversity of xylem vessels would be needed to transport water and meet evaporative demand as opposed to cultivars that were domesticated in temperate forest regions like 'Merlot'. These differences appear to expose 'Thompson Seedless' to high xylem vulnerability to cavitation.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"10 1","pages":"53-61"},"PeriodicalIF":0.0,"publicationDate":"2020-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80398453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-13DOI: 10.5073/VITIS.2020.59.35-39
Nadine Steinmetz, Gertraud Michl, M. Maixner, C. Hoffmann
The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.
{"title":"A rapid and inexpensive RNA-extraction method for high-throughput virus detection in grapevine","authors":"Nadine Steinmetz, Gertraud Michl, M. Maixner, C. Hoffmann","doi":"10.5073/VITIS.2020.59.35-39","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.35-39","url":null,"abstract":"The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"147 1","pages":"35-39"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77957403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-13DOI: 10.5073/VITIS.2020.59.41-46
M. Ferrer, G. Pereyra, Julia Salvarrey, L. Arrillaga, Mercedes Fourment
Climate variability influence on the vine is widely studied for its impact on grape final composition and quality. During 1994-2016, thermal and water regimes and their influence on grapevine yield, sanitary status and berry composition were analyzed for 'Tannat' grown in commercial vineyards in the south of Uruguay (Lat 34° 37' S; 56° 17' W). Statistical analysis showed that the principal component analysis (PCA) separated years in three groups: Group 1: rainfall over the growing season higher than the average, limited sanitary status, acidity and yield higher than average, lower sugar content, late harvest. Group 2: greater thermal conditions and water component lower than average, better sanitary status, sugar contents and acidity lower than average, early harvest. Group 3: thermal conditions lower than average, rainfall higher during budbreak-fruitset period and lower than average in the month before harvest, berry size and sugar contents greater than average. Correlations between climate, yield and berry quality variables were established and stages of greater sensitivity to these climate elements were determined. In the studied years, climate variability within the region was high and 'Tannat' showed to be strongly influenced by such variability.
{"title":"'Tannat' (Vitis vinifera L.) as a model of responses to climate variability","authors":"M. Ferrer, G. Pereyra, Julia Salvarrey, L. Arrillaga, Mercedes Fourment","doi":"10.5073/VITIS.2020.59.41-46","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.41-46","url":null,"abstract":"Climate variability influence on the vine is widely studied for its impact on grape final composition and quality. During 1994-2016, thermal and water regimes and their influence on grapevine yield, sanitary status and berry composition were analyzed for 'Tannat' grown in commercial vineyards in the south of Uruguay (Lat 34° 37' S; 56° 17' W). Statistical analysis showed that the principal component analysis (PCA) separated years in three groups: Group 1: rainfall over the growing season higher than the average, limited sanitary status, acidity and yield higher than average, lower sugar content, late harvest. Group 2: greater thermal conditions and water component lower than average, better sanitary status, sugar contents and acidity lower than average, early harvest. Group 3: thermal conditions lower than average, rainfall higher during budbreak-fruitset period and lower than average in the month before harvest, berry size and sugar contents greater than average. Correlations between climate, yield and berry quality variables were established and stages of greater sensitivity to these climate elements were determined. In the studied years, climate variability within the region was high and 'Tannat' showed to be strongly influenced by such variability.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"23 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90542875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-13DOI: 10.5073/VITIS.2020.59.1-8
Rui Wang, Quan Sun, Xu Xing
This study was designed to investigate the influence of grass and film mulching on grape (V. vinifera) fruit quality and root transcriptome. The groundcovers (plastic film and seeds of herba portulacae and E. humifusa Willd) were set or sawn on the ground under wine grape plants ('Cabernet Sauvignon'). Test plots in the control group were treated with clean tillage. Properties of plant biochemicals under covers and grape berry quality were determined after two years. RNA-seq was analyzed for grape roots under different treatments. E. humifusa Willd cover increased plant total N and P; and film mulching increased plant total P and decreased plant total K. Herba portulacae cover decreased berry tannin and increased anthocyanin, soluble solid and titratable acid; E. humifusa Willd cover increased anthocyanin, soluble solid and titratable acid. Film mulching increased the contents of berry total phenols, soluble solid, and decreased titratable acid significantly in comparison with clean tillage. Root RNA-seq showed that there were 1, 0, and 42 differently expressed genes (DEGs) in Herba portulacae, E. humifusa Willd and film cover, compared with clean tillage, respectively. Film mulching increased the expression of root high-affinity nitrate transporter 2.1 (NRT2.1), NRT2.4 and glutamine synthetase, which associated with 'Nitrogen metabolism', and decreased the expression of root small class I/II heat shock proteins. Plastic film mulching increased grapple quality properties via activating N metabolism. Film mulching was a more excellent mulching for improving grape quality than grass cover in Ningxia, China.
{"title":"Root transcriptome reveals responses to plastic film mulching and grass cover in wine grape 'Cabernet Sauvignon' root and berry","authors":"Rui Wang, Quan Sun, Xu Xing","doi":"10.5073/VITIS.2020.59.1-8","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.1-8","url":null,"abstract":"This study was designed to investigate the influence of grass and film mulching on grape (V. vinifera) fruit quality and root transcriptome. The groundcovers (plastic film and seeds of herba portulacae and E. humifusa Willd) were set or sawn on the ground under wine grape plants ('Cabernet Sauvignon'). Test plots in the control group were treated with clean tillage. Properties of plant biochemicals under covers and grape berry quality were determined after two years. RNA-seq was analyzed for grape roots under different treatments. E. humifusa Willd cover increased plant total N and P; and film mulching increased plant total P and decreased plant total K. Herba portulacae cover decreased berry tannin and increased anthocyanin, soluble solid and titratable acid; E. humifusa Willd cover increased anthocyanin, soluble solid and titratable acid. Film mulching increased the contents of berry total phenols, soluble solid, and decreased titratable acid significantly in comparison with clean tillage. Root RNA-seq showed that there were 1, 0, and 42 differently expressed genes (DEGs) in Herba portulacae, E. humifusa Willd and film cover, compared with clean tillage, respectively. Film mulching increased the expression of root high-affinity nitrate transporter 2.1 (NRT2.1), NRT2.4 and glutamine synthetase, which associated with 'Nitrogen metabolism', and decreased the expression of root small class I/II heat shock proteins. Plastic film mulching increased grapple quality properties via activating N metabolism. Film mulching was a more excellent mulching for improving grape quality than grass cover in Ningxia, China.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"31 1 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81838409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-13DOI: 10.5073/VITIS.2020.59.9-18
R. Tosin, I. Pôças, I. Gonçalves, M. Cunha
Hyperspectral data collected through a handheld spectroradiometer (400-1010 nm) were tested for assessing the grapevine predawn leaf water potential (ѱpd) measured by a Scholander chamber in two test sites of Douro wine region. The study was implemented in 2017, being a year with very hot and dry summer, conditions prone to severe water shortage. Three grapevine cultivars, 'Touriga Nacional', 'Touriga Franca' and 'Tinta Barroca' were sampled both in rainfed and irrigated vineyards, with a total of 325 plants assessed in four post-flowering dates. A large set of vegetation indices computed with the hyperspectral data and optimized for the ѱpd values, as well as structural variables, were used as predictors in the model. From a total of 631 possible predictors, four variables were selected based on a stepwise forward procedure and the Wald statistics: irrigation treatment, test site, Anthocyanin Reflectance Index Optimized (ARIopt_656,647) and Normalized Ratio Index (NRI711,700). An ordinal logistic regression model was calibrated using 70 % of the dataset randomly selected and the 30 of the remaining observations where used in model validation. The overall model accuracy obtained with the validation dataset was 73.2 %, with the class of ѱpd corresponding to the high-water deficit presenting a positive prediction value of 79.3 %. The accuracy and operability of this predictive model indicates good perspectives for its use in the monitoring of grapevine water status, and to support the irrigation tasks.
{"title":"Estimation of grapevine predawn leaf water potential based on hyperspectral reflectance data in Douro wine region","authors":"R. Tosin, I. Pôças, I. Gonçalves, M. Cunha","doi":"10.5073/VITIS.2020.59.9-18","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.9-18","url":null,"abstract":"Hyperspectral data collected through a handheld spectroradiometer (400-1010 nm) were tested for assessing the grapevine predawn leaf water potential (ѱpd) measured by a Scholander chamber in two test sites of Douro wine region. The study was implemented in 2017, being a year with very hot and dry summer, conditions prone to severe water shortage. Three grapevine cultivars, 'Touriga Nacional', 'Touriga Franca' and 'Tinta Barroca' were sampled both in rainfed and irrigated vineyards, with a total of 325 plants assessed in four post-flowering dates. A large set of vegetation indices computed with the hyperspectral data and optimized for the ѱpd values, as well as structural variables, were used as predictors in the model. From a total of 631 possible predictors, four variables were selected based on a stepwise forward procedure and the Wald statistics: irrigation treatment, test site, Anthocyanin Reflectance Index Optimized (ARIopt_656,647) and Normalized Ratio Index (NRI711,700). An ordinal logistic regression model was calibrated using 70 % of the dataset randomly selected and the 30 of the remaining observations where used in model validation. The overall model accuracy obtained with the validation dataset was 73.2 %, with the class of ѱpd corresponding to the high-water deficit presenting a positive prediction value of 79.3 %. The accuracy and operability of this predictive model indicates good perspectives for its use in the monitoring of grapevine water status, and to support the irrigation tasks.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"29 1","pages":"9-18"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83097832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-13DOI: 10.5073/VITIS.2020.59.19-28
Hong Lin, Yinshan Guo, Xiaoxu Yang, S. Kondo, Yuhui Zhao, Zhendong Liu, Kun Li, Xiu-wu Guo
Great progress has been made during the last decade in clarifying the molecular details of aroma accumulation in grape berries. However, the multigene complex controlling monoterpene accumulation in grape is not well understood. To shed light on this issue, the grapes of 149 F1 progenies from the cross 87-1 (Vitis vinifera L.) × 9-22 (Vitis vinifera L.) were characterized at the mature stage for three representative free monoterpenes during five growing seasons. A total of 202, 184 and 255 polymorphic SSR (simple sequence repeat) markers were contracted on the maternal 87-1, paternal 9-22 and consensus genetic maps, respectively. On the consensus map, we confirmed a major QTL (quantitative trait locus) for free linalool, nerol and α-terpineol content on linkage group (LG) 5, and a stable QTL for free linalool and α-terpineol was detected on LG 10. In addition, two new stable QTLs for free monoterpene (linalool, nerol and α-terpineol) contents were identified on LG 11 and LG 18 that explained up to 42.5 % of the total variance. Eleven promising candidate genes related to pentatricopeptide repeat (PPR)-containing proteins, seed maturation protein, RING finger protein, and AP2/ERF transcription factors might be potentially involved in monoterpene accumulation. The stable QTLs and candidate genes identified in this study provide new insights into free monoterpene accumulation in grape.
{"title":"QTL identification and candidate gene identification for monoterpene content in grape (Vitis vinifera L.) berries","authors":"Hong Lin, Yinshan Guo, Xiaoxu Yang, S. Kondo, Yuhui Zhao, Zhendong Liu, Kun Li, Xiu-wu Guo","doi":"10.5073/VITIS.2020.59.19-28","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.19-28","url":null,"abstract":"Great progress has been made during the last decade in clarifying the molecular details of aroma accumulation in grape berries. However, the multigene complex controlling monoterpene accumulation in grape is not well understood. To shed light on this issue, the grapes of 149 F1 progenies from the cross 87-1 (Vitis vinifera L.) × 9-22 (Vitis vinifera L.) were characterized at the mature stage for three representative free monoterpenes during five growing seasons. A total of 202, 184 and 255 polymorphic SSR (simple sequence repeat) markers were contracted on the maternal 87-1, paternal 9-22 and consensus genetic maps, respectively. On the consensus map, we confirmed a major QTL (quantitative trait locus) for free linalool, nerol and α-terpineol content on linkage group (LG) 5, and a stable QTL for free linalool and α-terpineol was detected on LG 10. In addition, two new stable QTLs for free monoterpene (linalool, nerol and α-terpineol) contents were identified on LG 11 and LG 18 that explained up to 42.5 % of the total variance. Eleven promising candidate genes related to pentatricopeptide repeat (PPR)-containing proteins, seed maturation protein, RING finger protein, and AP2/ERF transcription factors might be potentially involved in monoterpene accumulation. The stable QTLs and candidate genes identified in this study provide new insights into free monoterpene accumulation in grape.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"28 1","pages":"19-28"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91204119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-13DOI: 10.5073/VITIS.2020.59.29-34
N. Bertazzon, V. Forte, E. Angelini
Grapevine 'Pinot gris' virus (GPGV) is a newly emergent virus associated with the appearance of grapevine leaf mottling and deformation disease (GLMD). The worldwide spreading of this virus, and sometimes of the associated disease, that has occurred in the last years, requests further epidemiological studies to verify the progress of natural infection in the field. In the present paper, GPGV infection and spatiotemporal spreading of GLMD, investigated in two vineyards with high disease occurrence, confirmed an elevated presence of the virus in vineyards of Northeastern Italy, and revealed an increasing of symptomatic plants over the time. At the same time, the progress of natural infection was monitored after the placement of new grafted plants near the symptomatic grapevines in the infected vineyards. After three years, 76 % of the plantlets that were initially GPGV-free became GPGV-infected, giving an evidence of the fast transmission of GPGV in the field. Only 14 % of the plantlets, all collocated inside a patch with diseased plants, showed typical GLMD-symptoms. Interestingly, some plantlets, which were already GPGV-infected with the "asymptomatic" GPGV variant before planting in the field, did not become infected with the "symptomatic" viral wild variant after three years and never showed GLMD symptoms.
{"title":"Fast transmission of grapevine 'Pinot gris' virus (GPGV) in vineyard","authors":"N. Bertazzon, V. Forte, E. Angelini","doi":"10.5073/VITIS.2020.59.29-34","DOIUrl":"https://doi.org/10.5073/VITIS.2020.59.29-34","url":null,"abstract":"Grapevine 'Pinot gris' virus (GPGV) is a newly emergent virus associated with the appearance of grapevine leaf mottling and deformation disease (GLMD). The worldwide spreading of this virus, and sometimes of the associated disease, that has occurred in the last years, requests further epidemiological studies to verify the progress of natural infection in the field. In the present paper, GPGV infection and spatiotemporal spreading of GLMD, investigated in two vineyards with high disease occurrence, confirmed an elevated presence of the virus in vineyards of Northeastern Italy, and revealed an increasing of symptomatic plants over the time. At the same time, the progress of natural infection was monitored after the placement of new grafted plants near the symptomatic grapevines in the infected vineyards. After three years, 76 % of the plantlets that were initially GPGV-free became GPGV-infected, giving an evidence of the fast transmission of GPGV in the field. Only 14 % of the plantlets, all collocated inside a patch with diseased plants, showed typical GLMD-symptoms. Interestingly, some plantlets, which were already GPGV-infected with the \"asymptomatic\" GPGV variant before planting in the field, did not become infected with the \"symptomatic\" viral wild variant after three years and never showed GLMD symptoms.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"50 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83719180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-13DOI: 10.5073/VITIS.2019.58.SPECIAL-ISSUE.83-88
A. Ruiz-Rodríguez, C. Carrera, M. P. Lovillo, C. Barroso
Grapes grown in warm climates have shorter ripening periods. This makes the regular level of several compounds related to sensory properties in wine to remain low. Therefore, those grapes need to receive a particular treatment during winemaking processes if they have to reach the adequate content levels of colour and aroma related compounds. Applying ultrasound during some of the winemaking procedures may contribute to improving the maceration process, which would result in a higher recovery of compounds from grape skins and seeds to the must. This work studies the effect of applying ultrasound to 'Syrah' musts for two different time lengths during its alcoholic fermentation. The wines produced according to regular winemaking procedures (reference wines) were then compared to wines that had been produced under the effect of ultrasound for 30 to 60 min per day. The results showed that the wines produced using ultrasound had concentrations of volatile compounds higher than their reference wine. These data were consistent with the results from the tasting panel, where the judges highlighted the red fruit notes of the wines resulting from the application of ultrasound during the alcoholic fermentation. On the other hand, there were differences between the two wines resulting from applying ultrasound for two different lengths of time, applying ultrasound for 30 min per day proved to be more effective in terms of aroma than applying ultrasounds for 60 min per day. The conclusion of this research is that applying ultrasound during the alcoholic fermentation favours the extraction of volatile compounds. However different times can produce different results. Furthermore, an excessive application of ultrasound may lead to the degradation of some of the compounds of interest.
{"title":"Ultrasonic treatments during the alcoholic fermentation of red wines: effects on 'Syrah' wines","authors":"A. Ruiz-Rodríguez, C. Carrera, M. P. Lovillo, C. Barroso","doi":"10.5073/VITIS.2019.58.SPECIAL-ISSUE.83-88","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.SPECIAL-ISSUE.83-88","url":null,"abstract":"Grapes grown in warm climates have shorter ripening periods. This makes the regular level of several compounds related to sensory properties in wine to remain low. Therefore, those grapes need to receive a particular treatment during winemaking processes if they have to reach the adequate content levels of colour and aroma related compounds. Applying ultrasound during some of the winemaking procedures may contribute to improving the maceration process, which would result in a higher recovery of compounds from grape skins and seeds to the must. This work studies the effect of applying ultrasound to 'Syrah' musts for two different time lengths during its alcoholic fermentation. The wines produced according to regular winemaking procedures (reference wines) were then compared to wines that had been produced under the effect of ultrasound for 30 to 60 min per day. The results showed that the wines produced using ultrasound had concentrations of volatile compounds higher than their reference wine. These data were consistent with the results from the tasting panel, where the judges highlighted the red fruit notes of the wines resulting from the application of ultrasound during the alcoholic fermentation. On the other hand, there were differences between the two wines resulting from applying ultrasound for two different lengths of time, applying ultrasound for 30 min per day proved to be more effective in terms of aroma than applying ultrasounds for 60 min per day. The conclusion of this research is that applying ultrasound during the alcoholic fermentation favours the extraction of volatile compounds. However different times can produce different results. Furthermore, an excessive application of ultrasound may lead to the degradation of some of the compounds of interest.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"58 1","pages":"83-88"},"PeriodicalIF":0.0,"publicationDate":"2019-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77812815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-13DOI: 10.5073/VITIS.2019.58.SPECIAL-ISSUE.135-142
N. Lavado, David Uriarte Hernández, L. Mancha, D. Moreno, M. Sánchez, M. H. P. Losada
In warmer regions, fruit ripening in the wine grape tends to take place during the hottest part of the growing season. This can have negative consequences on the qualitative characteristics of the grape berries at harvest. Forcing vines to regrow can be an aggressive but effective technique to delay the harvest date, but needs to be evaluated carefully in each growing condition. In 2017, in an experimental vineyard in Extremadura, forcing was conducted 3 (F1 treatment) and 22 (F2 treatment) days after anthesis (May 18 and June 6) by hedging growing shoots to seven nodes and removing summer laterals, leaves and primary clusters. Vines grown using conventional practices were used for the Control treatment. Forcing delayed the harvest date from August 22 (Control) to September 14 (F1) and October 19 (F2). Shifting the berry growth period modified the duration of the different fruit development stages. Compared to the Control treatment, the F1 and F2 berries were smaller at harvest, but had similar skin weight percentages; however, the seed weight percentage of the F2 berries was higher. The differences in grape composition observed at harvest between the various treatments were further accentuated in the wines. At harvest, the F2 berries had significantly higher total polyphenol and anthocyanin content than the Control and F1 berries, which had similar values. In the wines, both F1 and F2 characteristics differed considerably from the Control, most notably in the high F2 tannin concentration. These preliminary results from the first year of study indicate the potential of this technique to obtain wine grapes with very different characteristics, offering new viticultural perspectives in warm climate areas.
{"title":"Effect of forcing vine regrowth on 'Tempranillo' (Vitis vinifera L.) berry development and quality in Extremadura","authors":"N. Lavado, David Uriarte Hernández, L. Mancha, D. Moreno, M. Sánchez, M. H. P. Losada","doi":"10.5073/VITIS.2019.58.SPECIAL-ISSUE.135-142","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.SPECIAL-ISSUE.135-142","url":null,"abstract":"In warmer regions, fruit ripening in the wine grape tends to take place during the hottest part of the growing season. This can have negative consequences on the qualitative characteristics of the grape berries at harvest. Forcing vines to regrow can be an aggressive but effective technique to delay the harvest date, but needs to be evaluated carefully in each growing condition. In 2017, in an experimental vineyard in Extremadura, forcing was conducted 3 (F1 treatment) and 22 (F2 treatment) days after anthesis (May 18 and June 6) by hedging growing shoots to seven nodes and removing summer laterals, leaves and primary clusters. Vines grown using conventional practices were used for the Control treatment. Forcing delayed the harvest date from August 22 (Control) to September 14 (F1) and October 19 (F2). Shifting the berry growth period modified the duration of the different fruit development stages. Compared to the Control treatment, the F1 and F2 berries were smaller at harvest, but had similar skin weight percentages; however, the seed weight percentage of the F2 berries was higher. The differences in grape composition observed at harvest between the various treatments were further accentuated in the wines. At harvest, the F2 berries had significantly higher total polyphenol and anthocyanin content than the Control and F1 berries, which had similar values. In the wines, both F1 and F2 characteristics differed considerably from the Control, most notably in the high F2 tannin concentration. These preliminary results from the first year of study indicate the potential of this technique to obtain wine grapes with very different characteristics, offering new viticultural perspectives in warm climate areas.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"9 1","pages":"135-142"},"PeriodicalIF":0.0,"publicationDate":"2019-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82359190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-13DOI: 10.5073/VITIS.2019.58.SPECIAL-ISSUE.33-38
S. Vélez, J. A. Rubio, M. I. Andres, E. Barajas
A classification between three vineyards belonging to the Appellation of Origin Rueda (Castilla y Leon, Spain) has been established in veraison to determine the productive capacities of each vineyard and to study their impact on the grape quality. Several open-access multispectral images obtained from the SENTINEL-2A satellite in the year 2016 were used to calculate the NDVI (Normalized Difference Vegetation Index), which provides information about the vigour of the vineyards. Eleven cloud-free images were assessed and based on the NDVI, three vigour levels were established: high vigour (0.356-0.458), medium vigour (0.285-0.355) and low vigour (0.166-0.284). A level of vigour was assigned to each vineyard according to the NDVI mean values of its pixels. Significant differences were found in the pruning wood weight and yield: high, medium and low vigour values were 2438, 1895 and 1487 kg·ha-1 and 15984, 12990 and 10576 kg·ha-1, respectively. The highest values of total acidity (6.04 g·L-1) and tartaric acid (9.05 g·L-1) have been obtained in low vigour, as well as the lowest values of pH (3.26), malic acid (0.42 g·L-1) and potassium (1640 ppm). Finally, one wine per vigour was produced and a tasting was carried out to check if the differences between the vineyards were perceptible. According to the obtained results, the NDVI is a good indicator to classify vineyards, finding notable differences between the experimental treatments studied.
{"title":"Agronomic classification between vineyards ('Verdejo') using NDVI and Sentinel-2 and evaluation of their wines","authors":"S. Vélez, J. A. Rubio, M. I. Andres, E. Barajas","doi":"10.5073/VITIS.2019.58.SPECIAL-ISSUE.33-38","DOIUrl":"https://doi.org/10.5073/VITIS.2019.58.SPECIAL-ISSUE.33-38","url":null,"abstract":"A classification between three vineyards belonging to the Appellation of Origin Rueda (Castilla y Leon, Spain) has been established in veraison to determine the productive capacities of each vineyard and to study their impact on the grape quality. Several open-access multispectral images obtained from the SENTINEL-2A satellite in the year 2016 were used to calculate the NDVI (Normalized Difference Vegetation Index), which provides information about the vigour of the vineyards. Eleven cloud-free images were assessed and based on the NDVI, three vigour levels were established: high vigour (0.356-0.458), medium vigour (0.285-0.355) and low vigour (0.166-0.284). A level of vigour was assigned to each vineyard according to the NDVI mean values of its pixels. Significant differences were found in the pruning wood weight and yield: high, medium and low vigour values were 2438, 1895 and 1487 kg·ha-1 and 15984, 12990 and 10576 kg·ha-1, respectively. The highest values of total acidity (6.04 g·L-1) and tartaric acid (9.05 g·L-1) have been obtained in low vigour, as well as the lowest values of pH (3.26), malic acid (0.42 g·L-1) and potassium (1640 ppm). Finally, one wine per vigour was produced and a tasting was carried out to check if the differences between the vineyards were perceptible. According to the obtained results, the NDVI is a good indicator to classify vineyards, finding notable differences between the experimental treatments studied.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"2 1","pages":"33-38"},"PeriodicalIF":0.0,"publicationDate":"2019-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79524948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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