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Biological performance of the predatory mite Neoseiulus barkeri Hughes (Phytoseiidae): a candidate for controlling of three mite species infesting grape trees 巴氏新绥螨(植物绥螨科)对葡萄树上三种螨的生物学性能研究
Pub Date : 2021-02-17 DOI: 10.5073/VITIS.2021.60.11-20
M. Al-Azzazy
The life history and predation rate of Neoseiulus barkeri (Phytoseiidae) feeding on Colomerus vitis (Eriophyidae), Tetranychus urticae (Tetranychidae) and Brevipalpus lewisi (Tenuipalpidae) were determined in the laboratory at constant temperatures of 25, 30 and 35 °C, relative humidity of 50 ± 5 % and a photoperiod of 16:8 h (light:dark). Within the temperature range studied, the increase of temperature from 25-35 °C led to a shortened development period and an increased total predation rate and reproduction. Survival during immature stages development surpassed 94 % at all the temperatures from 25 to 35 °C. The highest fecundity (59.50, 48.25 and 35.30 eggs per female) was recorded at 35 °C, while the minimum (40.25, 31.00 and 20.50 eggs per female) was at 25 °C when N. barkeri fed on C. vitis, T. urticae and B. lewisi, respectively. It is demonstrated in the life table parameters that when the predatory mite N. barkeri is fed on C. vitis, the highest reproduction rates (rm = 0.195, 0.210 and 0.232 females/female/day) are obtained, while feeding on B. lewisi gave the minimum of reproduction rates (rm = 0.095, 0.105 and 0.115) at 25 °C, 30 °C and 35 °C, respectively. The population of N. barkeri multiplied 20.45, 22.63 and 24.89 times in a generation time of 16.80, 14.75 and 12.50 days when fed on C. vitis at the same temperatures mentioned above, respectively, while N. barkeri multiplied 10.70, 12.88 and 14.36 times in a generation time of 23.20, 21.11 and 18.08 days when fed on B. lewisi at the same temperatures, respectively. This shows that N. barkeri is a promising control agent for C. vitis, T. urticae and B. lewisi on grape trees.
在恒温25℃、30℃和35℃,相对湿度50±5%,光照周期16:8 h(明暗)的条件下,测定了巴氏新绥螨(植物绥螨科)摄食绿蛾、荨麻叶螨(叶螨科)和柳氏短喙螨(栉螨科)的生活史和捕食率。在研究的温度范围内,温度升高25 ~ 35℃,导致发育周期缩短,总捕食率和繁殖率增加。在25 ~ 35℃的所有温度下,未成熟期的成活率均超过94%。在35℃条件下,barkeri N. vitis、T. urticae和B. lewisi的产卵量最高,分别为59.50、48.25和35.30个卵,最低,分别为40.25、31.00和20.50个卵。从生命表参数中可以发现,在25℃、30℃和35℃条件下,捕食螨barkeri在葡萄球菌上的繁殖率最高,分别为0.195、0.210和0.232只/只/d,而捕食螨lewisi的繁殖率最低,分别为0.095、0.105和0.115只/d。在相同温度下取食葡萄球菌时,巴氏易僵螨在16.80、14.75和12.50 d的世代内分别繁殖20.45、22.63和24.89倍;在相同温度下取食刘易斯白僵螨时,巴氏易僵螨在23.20、21.11和18.08 d的世代内繁殖10.70、12.88和14.36倍。这表明巴氏奈瑟菌是防治葡萄树上葡萄球菌、荨麻疹和刘易斯白僵菌的有效药剂。
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引用次数: 2
Projecting changes in phenology and grape composition of 'Tempranillo' and 'Grenache' varieties under climate warming in Rioja DOCa 气候变暖对里奥哈产区“丹美乐”和“歌海娜”葡萄物候和成分变化的影响
Pub Date : 2020-11-02 DOI: 10.5073/VITIS.2020.59.181-190
M. C. Ramos, F. Toda
The aim of this research was to predict the changes in vine phenology under future climate change of two red varieties, cultivated under rainfed conditions, and how grape composition can be affected. The research was conducted in Rioja Designation of Origin (DOCa), a viticultural area located in north central Spain, where red varieties represent near 90 % of the cultivated vineyards. The research focuses on 'Tempranillo' and 'Grenache', which represent about 85 % and 10 %, respectively, of cultivated red varieties in the area. The analysis included data related to vineyards located at different elevations and with different climatic conditions, recorded during the period 2008-2018. Phenological dates related to separated flowers (stage H), veraison (stage M) and maturity of the two varieties as well as the grape composition during the ripening period and at maturity were evaluated. The future scenarios were based on the predicted temperature and precipitation changes under two Representative Concentration Pathway (RCP) scenarios –RCP4.5 and RCP8.5-, which were simulated with an ensemble of models. Projections for 2050 and 2070 were made based on the observed phenological dates and the heat accumulation needed to reach each stage along the growing cycle. An advance of all phenological stages was projected, higher for veraison and maturity than for the earlier stages, and without large differences between both varieties but with differences at different elevations. Veraison is expected to be advanced up to 11 days for 2050 and up to 12 days for 2070 under the RCP4.5 scenario, while under the RCP8.5 scenario, the advance by 2070 could be up to 20 days for both varieties. For maturity, the advance could be up to 27 days for 'Tempranillo' and 25 days for 'Grenache', with differences between the cooler and the warmer areas. These changes resulted in a shortening of the periods between phenological stages, giving rise to maturity under warmer conditions. Based on the variability of grape composition observed in the warmer in relation to cooler years, and on the relationship with climate variables, a decrease in acidity as well as a reduction in the content of total anthocyanins is expected for both varieties, which may be higher for 'Grenache' than for 'Tempranillo'.
本研究旨在预测两种雨养红品种在未来气候变化下的葡萄物候变化,以及对葡萄成分的影响。这项研究是在里奥哈原产地指定区(DOCa)进行的,这是一个位于西班牙中北部的葡萄种植区,红色品种占种植葡萄园的近90%。研究的重点是“丹魄”和“歌海娜”,它们分别占该地区种植的红色品种的85%和10%。该分析包括2008年至2018年期间记录的与不同海拔和不同气候条件下的葡萄园相关的数据。对两个品种的分花期(H期)、期(M期)、成熟期的物候数据以及成熟期和成熟期的葡萄成分进行了评价。未来情景基于代表性浓度路径(Representative Concentration Pathway, rcp4.5)和RCP8.5-两种情景下的预测温度和降水变化,并采用多模式组合进行模拟。2050年和2070年的预估是基于观测到的物候日期和达到生长周期每个阶段所需的热量积累。预测各物候期均提前,变异和成熟度均高于前几个物候期,两个品种间差异不大,但不同海拔处存在差异。在RCP4.5情景下,到2050年,Veraison预计将提前11天,2070年将提前12天,而在RCP8.5情景下,到2070年,这两个品种的提前时间可能高达20天。就成熟期而言,“丹pranillo”最多可提前27天,“歌海娜”最多可提前25天,这在较冷和较热地区有所不同。这些变化导致物候阶段之间的周期缩短,在温暖的条件下成熟。根据在较暖年份与较冷年份所观察到的葡萄成分的可变性,以及与气候变量的关系,预计这两个品种的酸度和总花青素含量都将下降,“歌海娜”的含量可能高于“丹pranillo”。
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引用次数: 5
Ripening effect on the concentration of polyfunctional thiol precursors in 'Gewürztraminer' 成熟对‘gew<s:1> rztraminer’中多功能硫醇前体浓度的影响
Pub Date : 2020-10-29 DOI: 10.5073/VITIS.2020.59.149-153
G. Nicolini, L. Tonidandel, A. Barbero, M. Paolini, E. Celotti, M. Bottura, T. Román
The effect of ripeness on the concentration of polyfunctional thiol precursors was investigated in 'Gewurztraminer' juices in two vintages characterised by very different climate conditions. An incremental trend of glutathionyl-3-mercaptohexan-1-ol and cysteinyl-3-mercaptohexan-1-ol during ripening was observed. The increase in the last phase of maturation was noticeable in both vintages. The concentration of 4-S-glutathionyl-4-methylpentan-2-one and 4-S-cysteinyl-4-methylpentan-2-one was confirmed to be low in 'Gewurztraminer', although the latter was found at quantifiable levels in a couple of samples. The management of the harvest date appears to be highly important in order to exploit the potential grapefruit-like note related to polyfunctional thiols of little aromatic GWT wines, particularly in case of challenging vintage years.
在气候条件非常不同的两个年份中,研究了成熟度对“酒浆”果汁中多功能硫醇前体浓度的影响。在成熟过程中,谷胱甘肽-3-巯基己烷-1-醇和半胱氨酸-3-巯基己烷-1-醇呈增加趋势。这两个年份在成熟的最后阶段都有明显的增加。4- s -谷胱甘肽-4-甲基戊烷-2- 1和4- s -半胱氨酸-4-甲基戊烷-2- 1的浓度被证实在“Gewurztraminer”中很低,尽管后者在几个样品中被发现具有可量化的水平。采收日期的管理似乎是非常重要的,以开发潜在的葡萄柚的味道,与多功能性硫醇的小芳香GWT葡萄酒,特别是在具有挑战性的年份。
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引用次数: 0
Mechanical tests and definition of new indexes of grape berry firmness. Evolution of berry skin hardness during alcoholic fermentation 葡萄果实硬度新指标的力学试验及定义。酒精发酵过程中浆果果皮硬度的变化
Pub Date : 2020-10-29 DOI: 10.5073/VITIS.2020.59.163-168
P. Abbal, A. Vernhet, E. Abi-Habib, S. Carrillo, M. Ducasse, C. Legrand
The mechanical strength or firmness of a fruit is considered an important parameter to characterise its state of ripeness or conservation, as well as other parameters such as sugar level or color. The mechanical hardness of grapes influences the integrity and sanitary quality of the harvest. In this study, the mechanical characteristics of grapevine berries were studied at harvest time in order to determine their rheological properties (firmness and hardness of the berry skin) during alcoholic fermentation. Special indexes were defined measuring the energy needed to crush the berries to 50 % of their initial diameter, and applied successively to two different varieties. The entire berry firmness and the skin hardness were both different. Mechanical indexes linked to grape firmness were defined. Using these indexes, a significant effect on the firmness behavior due to variety was recorded: the skin of 'Grenache Noir' was found firmer and harder than 'Carignan Noir'. Furthermore, during the alcoholic fermentation, no change in skin hardness was observed for both varieties, despite changes in the composition of the must. These results give new information on mechanical properties of berries and could be used as an aid in the winemaking process. Indeed, they would probably help the winemaker to better choose the type of fermentation and maceration adapted to his grapes according to the type of wine he wishes to produce.
水果的机械强度或硬度被认为是表征其成熟或保存状态的重要参数,以及其他参数,如糖含量或颜色。葡萄的机械硬度影响收获的完整性和卫生质量。在本研究中,研究了葡萄果实在收获时的机械特性,以确定其在酒精发酵过程中的流变特性(浆果皮的硬度和硬度)。定义了特殊的指标,测量将浆果粉碎到其初始直径的50%所需的能量,并先后应用于两个不同的品种。果实的整体硬度和果皮硬度均有差异。定义了与葡萄硬度相关的机械指标。使用这些指标,记录了品种对紧实行为的显著影响:发现“黑歌海娜”的表皮比“黑丽酿”更紧实、更硬。此外,在酒精发酵过程中,尽管果皮的成分发生了变化,但两种品种的果皮硬度都没有变化。这些结果为浆果的机械特性提供了新的信息,可以作为酿酒过程的辅助手段。事实上,它们可能会帮助酿酒师根据他希望生产的葡萄酒类型,更好地选择适合他的葡萄的发酵和浸渍方式。
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引用次数: 2
Growth, heavy metal uptake, and photosynthesis in 'Paulsen 1103' (Vitis berlandieri x rupestris) grapevine rootstocks inoculated with arbuscular mycorrhizal fungi from vineyard soils with high copper contents 从高铜含量的葡萄园土壤中接种丛枝菌根真菌后,‘Paulsen 1103’(Vitis berlandieri x rupestris)葡萄砧木的生长、重金属吸收和光合作用
Pub Date : 2020-10-29 DOI: 10.5073/VITIS.2020.59.169-180
M. Betancur-Agudelo, E. Meyer, P. E. Lovato
Soils in old vineyards in southern Brazil have high copper accumulation due to fungicide applications over the years, which can affect physiology and growth of young grapevine plants. Arbuscular mycorrhizal fungi (AMF) alleviate toxic effects of metals and increase photosynthesis and plant growth. We evaluated whether inoculation with Rhizophagus clarus (Rh) from a mining area or with a trap-culture-enriched AMF community (Tc) isolated from a high-copper vineyard soil, improved growth and photosynthesis in grapevine rootstocks planted in young ( 60 years) vineyards soils of Vale da Uva Goethe, SC, Brazil. Mycorrhizal colonization was higher in grapevines installed in young vineyard soil than those planted in old vineyard soil. Plants grew more in the old vineyard soil than in the soil from a young vineyard, and that was related to plant nutrient concentration in the soil. In both soils, Tc-inoculated grapevines had higher photosynthetic activity, while those inoculated with R. clarus had higher carbon assimilation. In conclusion, grapevines showed a positive response to AMF inoculation in different soil conditions, and the native AMF community from high copper soils are promising for inoculation of grapevines.
巴西南部老葡萄园的土壤由于多年施用杀菌剂而具有高铜积累,这可能影响年轻葡萄植株的生理和生长。丛枝菌根真菌(AMF)减轻金属的毒性作用,促进光合作用和植物生长。我们评估了接种来自矿区的根噬菌(Rh)或从高铜葡萄园土壤中分离的富含诱杀培养的AMF群落(Tc)是否能改善巴西Vale da Uva Goethe年轻(60年)葡萄园土壤中种植的葡萄砧木的生长和光合作用。种植在年轻葡萄园土壤中的葡萄菌根定植量高于种植在老葡萄园土壤中的葡萄。植物在老葡萄园土壤中比在新葡萄园土壤中生长得多,这与土壤中植物养分浓度有关。在两种土壤中,接种了tc的葡萄具有更高的光合活性,而接种了红曲霉的葡萄具有更高的碳同化能力。综上所述,葡萄在不同土壤条件下对AMF接种均表现出积极响应,高铜土壤的原生AMF群落具有接种葡萄的良好前景。
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引用次数: 5
Diverse and strain-specific metabolites patterns induced by fungal endophytes in grape cells of different varieties 不同品种葡萄细胞中真菌内生菌诱导的不同和菌株特异性代谢模式
Pub Date : 2020-10-29 DOI: 10.5073/VITIS.2020.59.155-162
X. Pan, Ming-Quan Yuan, Jing-Chao Chen, Man Yu, J. Qu, Fang Liu, S. Xiang, You-yong Zhu, Ming-Zhi Yang
The potential for endophytes to initiate changes in host secondary metabolism has been well documented. However, the mechanisms underlying endophyte-plant metabolic interactions are still poorly understood. Here, we analysed the effects of fungal endophytes on the metabolite profiles of grape cells from two cultivars: 'Cabernet Sauvignon' (CS) and 'Rose honey' (RH). Our results clearly showed that co-culture with endophytic fungi greatly modified the metabolic profiles in grape cells of both varieties. Treatments with endophytic fungal strains caused the numbers of detected metabolites to vary from 10 to 19 in CS cells and from 8 to 14 in RH cells. In addition, 5 metabolites were detected in all CS cell samples, while 4 metabolites were detected in all RH cell samples. Some endophytic fungal strains could even introduce novel metabolites into the co-cultured grape cells. The metabolic profiles of grape leaves shaped by endophytic fungi exhibited host selectivity and fungal strain specificity. In this assay, the fungal strains RH32 (Alternaria sp.) and MDR36 (Colletotrichum sp.) triggered an increased response of the detected metabolites, including the greatest increase in the metabolite contents in grape cells of both cultivars. No obvious effects in terms of metabolite numbers and contents in grape cells when co-cultured with fungal strains RH7 (Epicoccum sp.) and RH48 (Colletotrichum sp.) were observed. The results of this experiment suggest that endophytic fungi could be used to control the metabolic profiles of grapes and thus increase grape quality.
内生菌引发宿主次生代谢变化的潜力已被充分证明。然而,潜在的内生植物代谢相互作用的机制仍然知之甚少。在这里,我们分析了真菌内生菌对“赤霞珠”(Cabernet Sauvignon, CS)和“玫瑰蜂蜜”(Rose honey, RH)两个葡萄品种的细胞代谢物谱的影响。我们的研究结果清楚地表明,与内生真菌共培养极大地改变了两个品种葡萄细胞的代谢谱。内生真菌菌株处理导致CS细胞检测到的代谢物数量从10到19不等,RH细胞检测到的代谢物数量从8到14不等。此外,CS细胞样品中检测到5种代谢物,RH细胞样品中检测到4种代谢物。一些内生真菌菌株甚至可以将新的代谢物引入共培养的葡萄细胞中。葡萄内生真菌叶片代谢谱表现出寄主选择性和真菌菌株特异性。在本实验中,真菌菌株RH32 (Alternaria sp.)和MDR36 (Colletotrichum sp.)对检测到的代谢物的反应增加,其中两个品种的葡萄细胞中代谢物含量的增加最大。与真菌菌株RH7 (Epicoccum sp.)和RH48 (Colletotrichum sp.)共培养对葡萄细胞代谢物数量和含量无明显影响。本试验结果提示,内生真菌可用于控制葡萄代谢谱,从而提高葡萄品质。
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引用次数: 0
Physiological impacts of early defoliation on the cold hardiness of grapevine (Vitis vinifera L.) 'Sultana' 早期落叶对葡萄抗寒性的生理影响“Sultana”
Pub Date : 2020-10-29 DOI: 10.5073/VITIS.2020.59.141-147
H. Sarikhani, H. Delgarm, S. Mansouri
Low winter temperatures are one of the limiting factors of grape production worldwide. This study was undertaken to inquire about the effects of postharvest early defoliation on the cold hardiness of grapevine. The grapevines samples, cv. Sultana, were defoliated at two stages (10 and 25 days after harvest), and then they were compared with natural leaf fall. Cane samples were collected in December 2017 and February 2018, and analyzed in terms of water content, soluble carbohydrate, and proline concentrations in both bud and cane tissues. The samples were then subjected to freezing treatments i. e. -8, -12, -15, -18, -21, and -24 °C for evaluating the levels of cold hardiness. Based on these results, early defoliation reduced proline and soluble carbohydrate concentrations but increased the water content compared to the control. Leaf removal also decreased abscisic acid concentration in the bud samples. Investigation of cold hardiness by electrolyte leakage and tetrazolium staining examinations showed that the defoliation decreased cold hardiness. Results demonstrated that leaf removal between the growing season and the beginning of the acclimation stage decreased the metabolite concentration in buds and canes and resulted to a reduction of cold hardiness.
冬季低温是全球葡萄生产的限制因素之一。本研究旨在探讨采收后早期落叶对葡萄抗寒性的影响。葡萄藤样本,cv。在收获后10天和25天分别进行落叶处理,并与自然落叶进行比较。研究人员于2017年12月和2018年2月采集了甘蔗样本,分析了甘蔗芽和甘蔗组织中的含水量、可溶性碳水化合物和脯氨酸浓度。然后将样品进行冷冻处理,即-8、-12、-15、-18、-21和-24°C,以评估耐寒性水平。基于这些结果,与对照相比,早期落叶降低了脯氨酸和可溶性碳水化合物浓度,但增加了水分含量。去叶也降低了芽样品中脱落酸的浓度。电解液渗漏和四氮唑染色对抗寒性的研究表明,落叶降低了抗寒性。结果表明,在生长季节和驯化阶段开始之间的叶片去除降低了芽和藤中的代谢物浓度,导致抗寒性降低。
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引用次数: 0
Confirming the function of a Final Bronze Age wine processing site in the Nuraghe Genna Maria in Villanovaforru (South Sardinia) 确认位于南撒丁岛Villanovaforru的Nuraghe Genna Maria最后青铜时代葡萄酒加工遗址的功能
Pub Date : 2020-07-30 DOI: 10.5073/VITIS.2020.59.93-100
G. Damasco, D. Delpiano, R. Larcher, T. Nardin, M. Perra, G. Lovicu
The stone artefact in the hut γ of the NuragheGenna Maria, object of this study, is part of a compound still unpublished today and dated to the Nuragic period. It was found during a 1991 excavation, revealing a situation unchanged since the collapse occurred between the 10th and 9th century B.C., thus preserving the situation at the time of the collapse to this day. The presence of tartaric acid - the marker considered to determinate the presence of wines or products deriving from grapes - has been determined using HPLC-DAD and UHPLC-HQOMS. So the findings under examination, together with the overall evaluation of the archaeological aspects examined, suggests to positively consider the stone artifact as a "laccus" (the latin word for wine presses, still used in the Sardinian language today ) for grape crushing. The internal slope of the floor of the "laccus" allowed the extraction of juice with rapid separation of juice from berry skins. The presence in Sardinia of a large number of "stone wine presses" ("palmenti" in Italian) such as that of the Nuraghe Genna Maria studied in this article, brings a contribution to their dating and confirm the existence of an oenological industry on the island in the Archaic period (9th-10th century B.C.).
这项研究的对象——努拉赫根纳玛丽亚(NuragheGenna Maria)小屋γ中的石制人工制品,是一个至今仍未发表的化合物的一部分,可追溯到努拉吉纪时期。在1991年的一次发掘中发现了它,从公元前10世纪到公元前9世纪发生坍塌以来,它的情况一直没有改变,因此保存了坍塌时的情况。酒石酸的存在-被认为是确定葡萄酒或葡萄衍生产品存在的标记-已使用HPLC-DAD和UHPLC-HQOMS测定。因此,研究中的发现,加上对考古方面的总体评估,表明可以肯定地认为这个石制工艺品是“laccus”(拉丁语中的葡萄酒压榨机,今天仍在撒丁岛语中使用),用于压榨葡萄。“laaccus”地板的内部斜坡允许提取果汁,并从浆果皮中快速分离果汁。在撒丁岛有大量的“石头压酒机”(意大利语为“palmenti”),例如本文研究的Nuraghe Genna Maria,这有助于确定它们的年代,并证实了在古代时期(公元前9 -10世纪)岛上存在酿酒工业。
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引用次数: 1
Using rootstocks to lower berry potassium concentrations in 'Cabernet Sauvignon' grapevines 利用砧木降低赤霞珠葡萄果实中的钾含量
Pub Date : 2020-07-30 DOI: 10.5073/VITIS.2020.59.117-126
Zeyu Xiao, Kerry DeGaris, T. Baby, Suzanne McLoughlin, B. Holzapfel, R. Walker, L. Schmidtke, S. Rogiers
Potassium is the most abundant cation in grape berries. It has important roles in grapevine physiology and winemaking. This study investigates the feasibility of using rootstocks to lower berry potassium concentrations ([K]) in 'Cabernet Sauvignon' grapevines. The ultimate target is to achieve lower pH and higher titratable acidity (TA) in grape juice so as to bring down the cost of acid adjustment during winemaking. The specific objective here is to provide new insights into the potential of particular rootstocks to modify K uptake by 'Cabernet Sauvignon' grapevines and their partitioning and accumulation into grape berries. The vineyard soils of a replicated rootstock trial located in the Limestone Coast of South Australia were characterised. Petiole, berry and juice nutrient content were assessed at oenological maturity of 'Cabernet Sauvignon' grown on eight different rootstocks. Rootstock had an impact on cations of the vegetative tissue of 'Cabernet Sauvignon', with Merbein 5512 having the lowest petiole [K]. The concentrations of major cations in the berry were, however, not altered by rootstock. While no particular rootstock stood out in limiting 'Cabernet Sauvignon' berry K accumulation, berries grown on the 'Borner' rootstock tended to have slightly lower concentrations (< 10 %) relative to vines on their own roots. Across the rootstocks, juice pH tended to increase with greater juice [K], while juice TA tended to decrease with greater juice [K]. It was found that juice TA was higher for the rootstocks 140 Ruggeri and 110 Richter, and juice pH tended to be lower for the rootstocks 110 Richter, 140 Ruggeri, Merbein 5512 and Merbein 5489. There was no effect of rootstock on total soluble solids.
钾是葡萄果实中含量最多的阳离子。它在葡萄的生理和酿酒中具有重要的作用。本研究探讨了利用砧木降低赤霞珠葡萄果实钾浓度([K])的可行性。最终目标是实现葡萄汁的低pH和高可滴定酸度(TA),从而降低酿酒过程中酸度调整的成本。本文的具体目标是提供新的见解,以了解特定砧木对“赤霞珠”葡萄吸收钾的潜力以及它们在葡萄果实中的分配和积累。位于南澳大利亚石灰石海岸的一个复制砧木试验的葡萄园土壤被表征。在8种不同砧木上生长的赤霞珠(Cabernet Sauvignon)在酿酒成熟时,对叶柄、浆果和果汁的营养成分进行了评估。砧木对赤霞珠(Cabernet Sauvignon)营养组织阳离子有影响,其中Merbein 5512的叶柄最低[K]。然而,砧木没有改变浆果中主要阳离子的浓度。虽然没有特定的砧木在限制“赤霞珠”浆果K积累方面表现突出,但“博纳”砧木上生长的浆果相对于自己根部的葡萄藤,其浓度略低(< 10%)。在整个砧木中,汁液pH值随着汁液[K]的增大而增加,汁液TA值随着汁液[K]的增大而降低。结果表明,140 Ruggeri和110 Richter的汁液TA较高,110 Richter、140 Ruggeri、Merbein 5512和Merbein 5489的汁液pH有降低的趋势。砧木对总可溶性固形物没有影响。
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引用次数: 4
Accumulated data and results from the recent study of dsRNA isolated from grapevines used in experiments of insect and graft transmission of 'Shiraz' disease 从葡萄中分离的dsRNA用于“设拉子”病昆虫和移植物传播实验的最新研究积累的数据和结果
Pub Date : 2020-07-30 DOI: 10.5073/VITIS.2020.59.111-116
D. Goszczynski
RT-PCR amplicons of dsRNA isolated from various grapevines, which were used in the experiments of transmission of 'Shiraz' disease (SD) from 'Cinsaut Blanc' clone P163/12 to SD-susceptible 'Merlot' and 'Shiraz' using mealybug Planococcus ficus and grafting were investigated. The amplicons were generated in RT-PCR based on virus-specific or random hexamers oligonucleotide primers. Standard molecular techniques and high-throughput sequencing (HTS), respectively, were applied. The results supported the hypothesis that GVA M5v variant present in 'Cinsaut Blanc' P163/12, which is a member of group II of GVA variants associated with SD, is crucial for developing this disease. HTS data did not reveal any other grapevine viruses besides GLRaV-3 and GVA in SD-affected grapevines, except for GVE which, however, was not present in all diseased plants.
采用RT-PCR技术,研究了从不同葡萄中分离到的dsRNA的扩增产物,这些葡萄从‘Cinsaut Blanc’无性系P163/12中分离到‘设拉子’病(SD),并利用粉蚧Planococcus ficus和嫁接将SD易感的‘梅洛’和‘设拉子’传播。扩增子是基于病毒特异性或随机六聚体寡核苷酸引物的RT-PCR产生的。分别采用标准分子技术和高通量测序技术。结果支持了GVA M5v变异存在于'Cinsaut Blanc' P163/12中的假设,该变异是与SD相关的GVA变异II组的成员,对该病的发生至关重要。HTS数据未发现除glav -3和GVA外的其他葡萄病毒,但GVE并不存在于所有病株中。
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Vitis: Journal of Grapevine Research
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