Pub Date : 2018-10-10DOI: 10.5073/VITIS.2018.57.125-128
M. Almasi
Grapevine fleck virus (GFkV) produces a ubiquitous disease, latent in grapevine causing simple or complex infections with other more dangerous viruses. The aim of the present study is to detect GFkV through application of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay; its efficiency has been contrasted with other procedures such as Double antibody sandwich ELISA (DAS-ELISA) and RT-polymerase chain reaction (PCR). The coat protein (CP) gene of the virus is basically used for designing the primers. Using hydroxynaphthol blue (HNB) Dye, RT-LAMP was placed in a water bath after the optimization was done. In order to detect GFkV easily and rapidly, a new immunocapture (IC)-RT-LAMP assay was developed as well; it was further compared with other assays. The results show RT-LAMP is an advantageous method because it is highly sensitive, quite cheap, user-friendly, and safe; in addition, it is performed quickly by visual detection and does not require RNA extraction (in IC-RT-LAMP).
{"title":"Development of reverse transcription loop mediated isothermal amplification assay for visual detection of Grapevine fleck virus (GFkV)","authors":"M. Almasi","doi":"10.5073/VITIS.2018.57.125-128","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.125-128","url":null,"abstract":"Grapevine fleck virus (GFkV) produces a ubiquitous disease, latent in grapevine causing simple or complex infections with other more dangerous viruses. The aim of the present study is to detect GFkV through application of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay; its efficiency has been contrasted with other procedures such as Double antibody sandwich ELISA (DAS-ELISA) and RT-polymerase chain reaction (PCR). The coat protein (CP) gene of the virus is basically used for designing the primers. Using hydroxynaphthol blue (HNB) Dye, RT-LAMP was placed in a water bath after the optimization was done. In order to detect GFkV easily and rapidly, a new immunocapture (IC)-RT-LAMP assay was developed as well; it was further compared with other assays. The results show RT-LAMP is an advantageous method because it is highly sensitive, quite cheap, user-friendly, and safe; in addition, it is performed quickly by visual detection and does not require RNA extraction (in IC-RT-LAMP).","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"5 1","pages":"125-128"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81271611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-10DOI: 10.5073/VITIS.2018.57.143-150
M. Venier, C. Agüero, A. Bermejillo, M. Filippini, M. Hanana, M. Walker, E. Blumwald, A. Dandekar
Several transgenic plant species expressing AtNHX1, coding for a vacuolar Na+/H+ antiporter from Arabidopsis thaliana, have shown their ability to cope with salinity. The aim of this study was to analyze the response of Vitis vinifera cv. 'Thompson Seedless' transformed with AtNHX1 to salt stress, using soil substrate or hydroponic media, and to compare the response with untransformed 'Thompson Seedless' and allegedly tolerant 'Criolla' cultivars: 'Pedro Gimenez' and 'Criolla Chica'. 'Thompson Seedless' embryogenic calli were transformed with Agrobacterium tumefaciens carrying AtNHX1 under the control of CaMV 35S promoter. Transgenic and untransformed plants were grown in a greenhouse under hydroponics or in pots with soil, and were subjected to increasing concentrations of sodium chloride (NaCl) up to 150 mM for a period of 7 weeks. Growth and toxicity symptoms were less affected in transgenics as compared to the untransformed grapevines, and transgenic lines had higher shoot length, leaf area and dry weights at the end of the experiment. Root concentrations of Na in transgenics were similar or lower than that observed in untransformed genotypes. Growth impairment and toxicity symptoms were observed in all genotypes under both conditions, but effects were more severe in plants growing with hydroponic culture. Potassium content and shoot to root dry weight ratio decreased with NaCl in hydroponics but not in pots. 'Criolla' cultivars grew less than the other genotypes, although 'Pedro Gimenez' always exhibited highest shoot/root ratios.
{"title":"Analysis of salinity tolerance of \"Vitis vinifera\" 'Thompson Seedless' transformed with \"AtNHX1\"","authors":"M. Venier, C. Agüero, A. Bermejillo, M. Filippini, M. Hanana, M. Walker, E. Blumwald, A. Dandekar","doi":"10.5073/VITIS.2018.57.143-150","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.143-150","url":null,"abstract":"Several transgenic plant species expressing AtNHX1, coding for a vacuolar Na+/H+ antiporter from Arabidopsis thaliana, have shown their ability to cope with salinity. The aim of this study was to analyze the response of Vitis vinifera cv. 'Thompson Seedless' transformed with AtNHX1 to salt stress, using soil substrate or hydroponic media, and to compare the response with untransformed 'Thompson Seedless' and allegedly tolerant 'Criolla' cultivars: 'Pedro Gimenez' and 'Criolla Chica'. 'Thompson Seedless' embryogenic calli were transformed with Agrobacterium tumefaciens carrying AtNHX1 under the control of CaMV 35S promoter. Transgenic and untransformed plants were grown in a greenhouse under hydroponics or in pots with soil, and were subjected to increasing concentrations of sodium chloride (NaCl) up to 150 mM for a period of 7 weeks. Growth and toxicity symptoms were less affected in transgenics as compared to the untransformed grapevines, and transgenic lines had higher shoot length, leaf area and dry weights at the end of the experiment. Root concentrations of Na in transgenics were similar or lower than that observed in untransformed genotypes. Growth impairment and toxicity symptoms were observed in all genotypes under both conditions, but effects were more severe in plants growing with hydroponic culture. Potassium content and shoot to root dry weight ratio decreased with NaCl in hydroponics but not in pots. 'Criolla' cultivars grew less than the other genotypes, although 'Pedro Gimenez' always exhibited highest shoot/root ratios.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"65 1","pages":"143-150"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83957385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-10DOI: 10.5073/VITIS.2018.57.151-157
Xutong Zhang, Peijie Gong, Y. Shi, Yuejin Wang, Chaohong Zhang
Sequence-Related Amplified Polymorphism (SRAP) markers were used to assess genetic inter-relationships among 39 grape genotypes. These included 22 indigenous Chinese grape species/varieties, the north American V. riparia and the European V. vinifera L. 'Thompson seedless' and 'Pinot noir'. Of the 72 SRAP primer combinations tested, 25 primers generated 135 reliable bands, with an average of 5.52 bands per primer pair. Further analysis shows that 106 of 135 bands were generated by 25 polymorphic primer pairs, with a polymorphism efficiency of 79 %. The similarity coefficients of SRAP polymorphism varied from 0.463 to 0.981 among the genotypes analysed. A dendrogram analysis divided the 39 Vitis accessions into 21 groups with similarity coefficients of 0.83. It reveals broadly similar genetic relationships among the genotypes examined to those previously determined using classical taxonomic methods. Our results define V. heyneana subsp. ficifolia and V. baihensis as subspecies of V. heyneana and V. bashanica, respectively. We question the placement of V. davidii var. cyanocarpa and V. davidii var. ningqiangensis as varieties in V. davidii.
{"title":"Genetic inter-relationships among Chinese wild grapes based on SRAP marker analyses","authors":"Xutong Zhang, Peijie Gong, Y. Shi, Yuejin Wang, Chaohong Zhang","doi":"10.5073/VITIS.2018.57.151-157","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.151-157","url":null,"abstract":"Sequence-Related Amplified Polymorphism (SRAP) markers were used to assess genetic inter-relationships among 39 grape genotypes. These included 22 indigenous Chinese grape species/varieties, the north American V. riparia and the European V. vinifera L. 'Thompson seedless' and 'Pinot noir'. Of the 72 SRAP primer combinations tested, 25 primers generated 135 reliable bands, with an average of 5.52 bands per primer pair. Further analysis shows that 106 of 135 bands were generated by 25 polymorphic primer pairs, with a polymorphism efficiency of 79 %. The similarity coefficients of SRAP polymorphism varied from 0.463 to 0.981 among the genotypes analysed. A dendrogram analysis divided the 39 Vitis accessions into 21 groups with similarity coefficients of 0.83. It reveals broadly similar genetic relationships among the genotypes examined to those previously determined using classical taxonomic methods. Our results define V. heyneana subsp. ficifolia and V. baihensis as subspecies of V. heyneana and V. bashanica, respectively. We question the placement of V. davidii var. cyanocarpa and V. davidii var. ningqiangensis as varieties in V. davidii.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"29 1","pages":"151-157"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84059240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-06DOI: 10.5073/vitis.2018.57.111-117
K. Labonova, M. Sineux, O. Zekri
For an easier identification and quantification of R. irregularis in grapevine, a molecular tool was developed so that each DNA concentration calculated would relate to the degree of root system colonization. To correlate the results obtained by qPCR and microscopy, a different approach for the visualization technique was chosen. It combined the detailed standard method of evaluation on microscopic slides with the global magnifying glass evaluation method in the grids. The global assessment sampling was closer to the qPCR sampling that was made on a representative fraction of the whole root system. This fact became the base of successful correlation between microscopy and qPCR. The result of these measures were the attribution of an average qPCR value to each level of colonization defined as five different classes. Around 0.66 ng·µL-1, the DNA concentration corresponded to the first contacts between the fungus and the grapevine roots (class M1), while around 42 ng·µL-1 it accounted for the beginning of the mycorrhizal symbiosis (class M2). A satisfactory mycorrhization level could be concluded from a 258 ng·µL-1 DNA concentration (class M3), while all values above 563 ng·µL-1 (class M4) showed a full mycorrhization level. The development of this qPCR tool allowed the fast and accurate evaluation of the mycorrhization level in the root system without having to realize any microscopic observation.
{"title":"Correlation between the microscopy and qPCR methods (SYBR Green) to detect and quantify Rhizophagus irregularis in grapevine roots","authors":"K. Labonova, M. Sineux, O. Zekri","doi":"10.5073/vitis.2018.57.111-117","DOIUrl":"https://doi.org/10.5073/vitis.2018.57.111-117","url":null,"abstract":"For an easier identification and quantification of R. irregularis in grapevine, a molecular tool was developed so that each DNA concentration calculated would relate to the degree of root system colonization. To correlate the results obtained by qPCR and microscopy, a different approach for the visualization technique was chosen. It combined the detailed standard method of evaluation on microscopic slides with the global magnifying glass evaluation method in the grids. The global assessment sampling was closer to the qPCR sampling that was made on a representative fraction of the whole root system. This fact became the base of successful correlation between microscopy and qPCR. The result of these measures were the attribution of an average qPCR value to each level of colonization defined as five different classes. Around 0.66 ng·µL-1, the DNA concentration corresponded to the first contacts between the fungus and the grapevine roots (class M1), while around 42 ng·µL-1 it accounted for the beginning of the mycorrhizal symbiosis (class M2). A satisfactory mycorrhization level could be concluded from a 258 ng·µL-1 DNA concentration (class M3), while all values above 563 ng·µL-1 (class M4) showed a full mycorrhization level. The development of this qPCR tool allowed the fast and accurate evaluation of the mycorrhization level in the root system without having to realize any microscopic observation.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"20 1","pages":"111-117"},"PeriodicalIF":0.0,"publicationDate":"2018-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82896540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-06DOI: 10.5073/VITIS.2018.57.119-123
E. Zyprian, R. Eibach, O. Trapp, F. Schwander, R. Töpfer
Viticulture is of high economic value. Traditional grapevine cultivars (Vitis vinifera L.) are used in adaptation to the climatic conditions from Northern to Southern European growing areas. However, the recent trend of global warming causes quality deficits due to a shift of the plant's development to earlier times in the year. As a consequence, premature ripening happens under warm temperatures and interferes with the balanced accumulation of sugars, acids, aroma profiles and berry coloration. Modern grapevine breeding is challenged to include the ripening behavior as an important trait (besides pathogen resistance and other characteristics) into the development of novel cultivars well suited for sustainable viticulture. Breeders nowadays apply molecular markers to follow the introgression of desired traits on the genetic level. Previous work has identified a molecular tag on grapevine chromosome 16 strongly linked to the time of veraison, the start of the second phase of berry ripening, in a segregating cross population. In this study we analyzed the transferability of this marker to a set of 36 grapevine cultivars commonly used in German viticulture. Association analysis verified the predictive character of the time point of veraison for maturation time and confirmed the diagnostic potential of the veraison-linked marker in this extended set of cultivars.
{"title":"Grapevine breeding under climate change: Applicability of a molecular marker linked to véraison","authors":"E. Zyprian, R. Eibach, O. Trapp, F. Schwander, R. Töpfer","doi":"10.5073/VITIS.2018.57.119-123","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.119-123","url":null,"abstract":"Viticulture is of high economic value. Traditional grapevine cultivars (Vitis vinifera L.) are used in adaptation to the climatic conditions from Northern to Southern European growing areas. However, the recent trend of global warming causes quality deficits due to a shift of the plant's development to earlier times in the year. As a consequence, premature ripening happens under warm temperatures and interferes with the balanced accumulation of sugars, acids, aroma profiles and berry coloration. Modern grapevine breeding is challenged to include the ripening behavior as an important trait (besides pathogen resistance and other characteristics) into the development of novel cultivars well suited for sustainable viticulture. Breeders nowadays apply molecular markers to follow the introgression of desired traits on the genetic level. Previous work has identified a molecular tag on grapevine chromosome 16 strongly linked to the time of veraison, the start of the second phase of berry ripening, in a segregating cross population. In this study we analyzed the transferability of this marker to a set of 36 grapevine cultivars commonly used in German viticulture. Association analysis verified the predictive character of the time point of veraison for maturation time and confirmed the diagnostic potential of the veraison-linked marker in this extended set of cultivars.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"110 1","pages":"119-123"},"PeriodicalIF":0.0,"publicationDate":"2018-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81434762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.5073/VITIS.2018.57.103-109
Joanna E. Jones, Fiona Kerslake, R. Dambergs, D. Close
Winter pruning is the principal method for regulating yield in viticulture. The aim of this work was to investigate the effectiveness of cane and spur pruning on yield, and on grape and wine composition. Cane and spur pruning were investigated in Vitis vinifera L. 'Pinot noir' and 'Chardonnay' vertically-shoot-positioned vines over three seasons. Effects on vine carbohydrates, yield components, leaf area, grape and base wine composition were determined. The canopies of spur pruned vines established more rapidly than cane pruned vines in the 2009/10 season, for both 'Pinot noir' and 'Chardonnay'. The canopies were denser under spur pruning than cane pruning. Pruning treatment had no effect on total yield for either cultivar in any of the three seasons. Total soluble solids (TSS) and titratable acidity were unaffected by pruning treatment, except in 2012 where TSS and pH were higher for spur pruned 'Chardonnay' vines. Apart from spur pruned 'Pinot noir' vine wood being higher in starch in the winter of 2011, overwintering starch and soluble sugar concentrations were not different between pruning treatments for 'Pinot noir' and 'Chardonnay'. Although not different in yield or basic fruit composition, fruit from spur pruned vines resulted in distinctly different phenolic profiles of base wines, with cane pruning appearing to negatively impact on the low molecular weight phenolics in the wine. The results presented here provide confidence that quality is not lessened, in fact could be improved, by shifting from the industry norm of cane to spur pruning for sparkling wine production in cool climates.
{"title":"Spur pruning leads to distinctly different phenolic profiles of base sparkling wines than cane pruning","authors":"Joanna E. Jones, Fiona Kerslake, R. Dambergs, D. Close","doi":"10.5073/VITIS.2018.57.103-109","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.103-109","url":null,"abstract":"Winter pruning is the principal method for regulating yield in viticulture. The aim of this work was to investigate the effectiveness of cane and spur pruning on yield, and on grape and wine composition. Cane and spur pruning were investigated in Vitis vinifera L. 'Pinot noir' and 'Chardonnay' vertically-shoot-positioned vines over three seasons. Effects on vine carbohydrates, yield components, leaf area, grape and base wine composition were determined. The canopies of spur pruned vines established more rapidly than cane pruned vines in the 2009/10 season, for both 'Pinot noir' and 'Chardonnay'. The canopies were denser under spur pruning than cane pruning. Pruning treatment had no effect on total yield for either cultivar in any of the three seasons. Total soluble solids (TSS) and titratable acidity were unaffected by pruning treatment, except in 2012 where TSS and pH were higher for spur pruned 'Chardonnay' vines. Apart from spur pruned 'Pinot noir' vine wood being higher in starch in the winter of 2011, overwintering starch and soluble sugar concentrations were not different between pruning treatments for 'Pinot noir' and 'Chardonnay'. Although not different in yield or basic fruit composition, fruit from spur pruned vines resulted in distinctly different phenolic profiles of base wines, with cane pruning appearing to negatively impact on the low molecular weight phenolics in the wine. The results presented here provide confidence that quality is not lessened, in fact could be improved, by shifting from the industry norm of cane to spur pruning for sparkling wine production in cool climates.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"358 1","pages":"103-109"},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87017480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-29DOI: 10.5073/VITIS.2018.57.93-101
A. J. Cantizano, A. Luján, R. Arroyo-García
A collection of 317 table-grape accessions preserved in the Vitis Germplasm Bank at the Rancho de la Merced (IFAPA, Jerez de la Frontera, Spain) were genotyped at 20 microsatellite loci. A total of 264 different genotypes were obtained. Among them, 66 are presented for the first time. Twenty-one accession names are considered new synonyms, fourteen homonyms and 48 can be considered as "denomination mistakes" in the Rancho de la Merced collection.
对保存在Rancho de la Merced (IFAPA, Jerez de la Frontera, Spain)葡萄种质库中的317份鲜食葡萄进行了20个微卫星位点的基因分型。共获得264个不同的基因型。其中，首次亮相的有66个。在Rancho de la Merced收藏中，21个加入名称被认为是新的同义词，14个同音异义，48个可以被认为是“命名错误”。
{"title":"Molecular characterization of table grape varieties preserved in the Rancho de la Merced Grapevine Germplasm Bank (Spain)","authors":"A. J. Cantizano, A. Luján, R. Arroyo-García","doi":"10.5073/VITIS.2018.57.93-101","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.93-101","url":null,"abstract":"A collection of 317 table-grape accessions preserved in the Vitis Germplasm Bank at the Rancho de la Merced (IFAPA, Jerez de la Frontera, Spain) were genotyped at 20 microsatellite loci. A total of 264 different genotypes were obtained. Among them, 66 are presented for the first time. Twenty-one accession names are considered new synonyms, fourteen homonyms and 48 can be considered as \"denomination mistakes\" in the Rancho de la Merced collection.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"26 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85284099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-29DOI: 10.5073/VITIS.2018.57.83-91
M. J. García-Esparza, I. Abrisqueta, I. Escriche, D. Intrigliolo, I. Alvarez, V. Lizama
Aroma compounds and skin and seed polyphenols are determinants of wine composition. The aim of this study was to determine the effect of different post-veraison deficit irrigation strategies on volatile profile and the chemical composition of grape skin and seeds in a 'Cabernet Sauvignon' vineyard in Valencia (Spain). Besides a non-irrigated regime (rainfed), irrigation treatments consisted of replacing 25, 50 and 75 % of the estimated crop evapotranspiration (ETC). When compared to rainfed vines, watering during post-veraison at 75 % of the ETC, decreased concentrations of alcohols but increased those of aldehydes such as hexanal, related to herbaceous (non-desirable) aromas in wines. Irrigating at 25 % or 50 % of ETC resulted in similar concentrations of grape volatile compounds than rainfed vines. There was also a general trend in a reduction in skin to flesh ratio as irrigation regime increased. The concentration of skin anthocyanins and tannins increased with water applications, but seed tannins decreased in the most irrigated regimes. This suggests different effects of water stress on skin and seed polyphenol synthesis and accumulation. For the tannin content, water stress provoked higher tannin mean degree polymerization values, which positively affect must astringency. Under the experimental conditions of the present study, watering at 50 % ETC during post-veraison is the recommended irrigation strategy for optimizing grape composition and improving yield in comparison with rainfed vines.
{"title":"Volatile compounds and phenolic composition of skins and seeds of 'Cabernet Sauvignon' grapes under different deficit irrigation regimes","authors":"M. J. García-Esparza, I. Abrisqueta, I. Escriche, D. Intrigliolo, I. Alvarez, V. Lizama","doi":"10.5073/VITIS.2018.57.83-91","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.83-91","url":null,"abstract":"Aroma compounds and skin and seed polyphenols are determinants of wine composition. The aim of this study was to determine the effect of different post-veraison deficit irrigation strategies on volatile profile and the chemical composition of grape skin and seeds in a 'Cabernet Sauvignon' vineyard in Valencia (Spain). Besides a non-irrigated regime (rainfed), irrigation treatments consisted of replacing 25, 50 and 75 % of the estimated crop evapotranspiration (ETC). When compared to rainfed vines, watering during post-veraison at 75 % of the ETC, decreased concentrations of alcohols but increased those of aldehydes such as hexanal, related to herbaceous (non-desirable) aromas in wines. Irrigating at 25 % or 50 % of ETC resulted in similar concentrations of grape volatile compounds than rainfed vines. There was also a general trend in a reduction in skin to flesh ratio as irrigation regime increased. The concentration of skin anthocyanins and tannins increased with water applications, but seed tannins decreased in the most irrigated regimes. This suggests different effects of water stress on skin and seed polyphenol synthesis and accumulation. For the tannin content, water stress provoked higher tannin mean degree polymerization values, which positively affect must astringency. Under the experimental conditions of the present study, watering at 50 % ETC during post-veraison is the recommended irrigation strategy for optimizing grape composition and improving yield in comparison with rainfed vines.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"32 1","pages":"83-91"},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73368251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-23DOI: 10.5073/VITIS.2018.57.75-81
Q. Zha, X. Xi, Yani He, A. Jiang
Temperature is a crucial factor limiting plant growth. Grapevine is frequently subjected to high temperature during its maturation stage, and this seriously influences grape growth and development. Here, we selected 68 grapevine varieties and examined the heat damage index, relative electrolyte leakage, and Fv/Fm after exposure to 50 °C. 'Red Seedless', 'Hong Yuli', 'At Suma', 'Hupei 3#', and 'Tamina' were tolerant to high-temperature stress; however, 'Brazil', 'Shenfeng', 'Gold Finger', 'Heimeixiang', 'Kaiji', and 'Zuijinxiang' varieties were sensitive to high-temperature stress and died after exposure to 50 °C. Our findings provide a valuable insight into resistance breeding programs for grapevine.
{"title":"Comprehensive evaluation of heat resistance in 68 Vitis germplasm resources","authors":"Q. Zha, X. Xi, Yani He, A. Jiang","doi":"10.5073/VITIS.2018.57.75-81","DOIUrl":"https://doi.org/10.5073/VITIS.2018.57.75-81","url":null,"abstract":"Temperature is a crucial factor limiting plant growth. Grapevine is frequently subjected to high temperature during its maturation stage, and this seriously influences grape growth and development. Here, we selected 68 grapevine varieties and examined the heat damage index, relative electrolyte leakage, and Fv/Fm after exposure to 50 °C. 'Red Seedless', 'Hong Yuli', 'At Suma', 'Hupei 3#', and 'Tamina' were tolerant to high-temperature stress; however, 'Brazil', 'Shenfeng', 'Gold Finger', 'Heimeixiang', 'Kaiji', and 'Zuijinxiang' varieties were sensitive to high-temperature stress and died after exposure to 50 °C. Our findings provide a valuable insight into resistance breeding programs for grapevine.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"2 1","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"2018-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80088567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-17DOI: 10.5073/vitis.2018.57.41-46
T. Román, G. Nicolini, L. Barp, M. Malacarne, F. Tait, R. Larcher
Shikimic acid (SHA) has been used for years as variety marker in fraud control, especially for 'Pinot Noir', 'Pinot Gris' and 'Pinot Blanc' with very low amounts. Until now no data for the hybrid grapes 'Bronner', 'Helios', 'Johanniter', 'Muscaris', 'Solaris' and 'Souvignier Gris' from the Viticultural Institute of Freiburg (Germany) were published. These white varieties with resistance against downy and powdery mildew are increasingly planted in some Alpine Italian regions. Data obtained from white wines of different vintages and vineyards in Trentino by HPLC showed that 'Johanniter' had the highest average content of SHA, while 'Solaris' and 'Muscaris' had much lower levels. These data are compared with previously published data for Italian wines of 19 white varieties and new data for 'Muller-Thurgau' and 'Yellow Muscat' wines. For a subset of 10 grape batches the influence of four different winemaking protocols on the amount of SHA was investigated. Increasing levels were found in the expected order from direct pressing to pressing of crushed-destemmed grapes (+28 %), short maceration of crushed-destemmed grapes before pressing (+37 %) and a 7-day skin-contact fermentation (+107 %).
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