The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury (EBI) following hemorrhagic stroke. Sprague-Dawley (SD) rats (n = 48) were randomly divided into sham operation (sham group), subarachnoid hemorrhage (SAH) model (SAH group), normal saline (NS group), and Ginaton (Extract of Ginkgo Biloba Leaves Drops) intervention (gin group) groups. MCP-1 mRNA and tumor necrosis factor levels were detected using reverse transcription–polymerase chain reaction. The relative expression of mRNA was detected by Western blotting. (1) Compared with the sham group, the SAH, NS, and gin groups had different degrees of neurological dysfunction. Compared with the SAH and NS groups, the neurological deficits in the gin group were significantly improved (P < 0.05). (2) Compared with the sham group, the relative expression levels of MCP-1 mRNA in the SAH, NS, and gin groups were 5.1 ± 0.9, 3.4 ± 0.6, and 2.5 ± 0.4, respectively; the relative expression levels of mRNA were 13.3 ± 2.4, 11.2 ± 1.8, and 3.8 ± 0.6, respectively. (3) The apoptosis rates of brain tissue in the sham, SAH, NS, and gin groups were 4.8 ± 0.7, 54.2 ± 10.3, 50.1 ± 7.4, and 28.4 ± 4.5, respectively. (4) Western blot showed that the relative expression levels of toll-like receptor-4 (TLR-4) protein in the sham, SAH, NS, and gin groups were 0.29 ± 0.03, 0.87 ± 0.15, 0.65 ± 0.13, and 0.41 ± 0.17, respectively; the relative expression levels of B protein were 0.21 ± 0.04, 0.96 ± 0.14, 0.73 ± 0.18, and 0.30 ± 0.05, respectively. Gin treatment could inhibit TLR-4 and nuclear factor-κB (NF-κB) protein expression. Dona tablets may inhibit activation of the NF-κB signaling pathway, and SAH-induced inflammatory response, so as to reduce cerebral vasospasm and EBI.
{"title":"Study on the Effect of Ginaton on Reducing Cerebral Vasospasm and Early Brain Injury after Hemorrhagic Stroke by Inhibiting Inflammatory Response","authors":"Xue-Bo Pang, Xiao-Lin Zhang, Mei-Rong Wang, Ying Yuan, Xiao Zhang","doi":"10.4103/2311-8571.393753","DOIUrl":"https://doi.org/10.4103/2311-8571.393753","url":null,"abstract":"\u0000 \u0000 \u0000 The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury (EBI) following hemorrhagic stroke.\u0000 \u0000 \u0000 \u0000 Sprague-Dawley (SD) rats (n = 48) were randomly divided into sham operation (sham group), subarachnoid hemorrhage (SAH) model (SAH group), normal saline (NS group), and Ginaton (Extract of Ginkgo Biloba Leaves Drops) intervention (gin group) groups. MCP-1 mRNA and tumor necrosis factor levels were detected using reverse transcription–polymerase chain reaction. The relative expression of mRNA was detected by Western blotting.\u0000 \u0000 \u0000 \u0000 (1) Compared with the sham group, the SAH, NS, and gin groups had different degrees of neurological dysfunction. Compared with the SAH and NS groups, the neurological deficits in the gin group were significantly improved (P < 0.05). (2) Compared with the sham group, the relative expression levels of MCP-1 mRNA in the SAH, NS, and gin groups were 5.1 ± 0.9, 3.4 ± 0.6, and 2.5 ± 0.4, respectively; the relative expression levels of mRNA were 13.3 ± 2.4, 11.2 ± 1.8, and 3.8 ± 0.6, respectively. (3) The apoptosis rates of brain tissue in the sham, SAH, NS, and gin groups were 4.8 ± 0.7, 54.2 ± 10.3, 50.1 ± 7.4, and 28.4 ± 4.5, respectively. (4) Western blot showed that the relative expression levels of toll-like receptor-4 (TLR-4) protein in the sham, SAH, NS, and gin groups were 0.29 ± 0.03, 0.87 ± 0.15, 0.65 ± 0.13, and 0.41 ± 0.17, respectively; the relative expression levels of B protein were 0.21 ± 0.04, 0.96 ± 0.14, 0.73 ± 0.18, and 0.30 ± 0.05, respectively. Gin treatment could inhibit TLR-4 and nuclear factor-κB (NF-κB) protein expression.\u0000 \u0000 \u0000 \u0000 Dona tablets may inhibit activation of the NF-κB signaling pathway, and SAH-induced inflammatory response, so as to reduce cerebral vasospasm and EBI.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139791098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury (EBI) following hemorrhagic stroke. Sprague-Dawley (SD) rats (n = 48) were randomly divided into sham operation (sham group), subarachnoid hemorrhage (SAH) model (SAH group), normal saline (NS group), and Ginaton (Extract of Ginkgo Biloba Leaves Drops) intervention (gin group) groups. MCP-1 mRNA and tumor necrosis factor levels were detected using reverse transcription–polymerase chain reaction. The relative expression of mRNA was detected by Western blotting. (1) Compared with the sham group, the SAH, NS, and gin groups had different degrees of neurological dysfunction. Compared with the SAH and NS groups, the neurological deficits in the gin group were significantly improved (P < 0.05). (2) Compared with the sham group, the relative expression levels of MCP-1 mRNA in the SAH, NS, and gin groups were 5.1 ± 0.9, 3.4 ± 0.6, and 2.5 ± 0.4, respectively; the relative expression levels of mRNA were 13.3 ± 2.4, 11.2 ± 1.8, and 3.8 ± 0.6, respectively. (3) The apoptosis rates of brain tissue in the sham, SAH, NS, and gin groups were 4.8 ± 0.7, 54.2 ± 10.3, 50.1 ± 7.4, and 28.4 ± 4.5, respectively. (4) Western blot showed that the relative expression levels of toll-like receptor-4 (TLR-4) protein in the sham, SAH, NS, and gin groups were 0.29 ± 0.03, 0.87 ± 0.15, 0.65 ± 0.13, and 0.41 ± 0.17, respectively; the relative expression levels of B protein were 0.21 ± 0.04, 0.96 ± 0.14, 0.73 ± 0.18, and 0.30 ± 0.05, respectively. Gin treatment could inhibit TLR-4 and nuclear factor-κB (NF-κB) protein expression. Dona tablets may inhibit activation of the NF-κB signaling pathway, and SAH-induced inflammatory response, so as to reduce cerebral vasospasm and EBI.
{"title":"Study on the Effect of Ginaton on Reducing Cerebral Vasospasm and Early Brain Injury after Hemorrhagic Stroke by Inhibiting Inflammatory Response","authors":"Xue-Bo Pang, Xiao-Lin Zhang, Mei-Rong Wang, Ying Yuan, Xiao Zhang","doi":"10.4103/2311-8571.393753","DOIUrl":"https://doi.org/10.4103/2311-8571.393753","url":null,"abstract":"\u0000 \u0000 \u0000 The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury (EBI) following hemorrhagic stroke.\u0000 \u0000 \u0000 \u0000 Sprague-Dawley (SD) rats (n = 48) were randomly divided into sham operation (sham group), subarachnoid hemorrhage (SAH) model (SAH group), normal saline (NS group), and Ginaton (Extract of Ginkgo Biloba Leaves Drops) intervention (gin group) groups. MCP-1 mRNA and tumor necrosis factor levels were detected using reverse transcription–polymerase chain reaction. The relative expression of mRNA was detected by Western blotting.\u0000 \u0000 \u0000 \u0000 (1) Compared with the sham group, the SAH, NS, and gin groups had different degrees of neurological dysfunction. Compared with the SAH and NS groups, the neurological deficits in the gin group were significantly improved (P < 0.05). (2) Compared with the sham group, the relative expression levels of MCP-1 mRNA in the SAH, NS, and gin groups were 5.1 ± 0.9, 3.4 ± 0.6, and 2.5 ± 0.4, respectively; the relative expression levels of mRNA were 13.3 ± 2.4, 11.2 ± 1.8, and 3.8 ± 0.6, respectively. (3) The apoptosis rates of brain tissue in the sham, SAH, NS, and gin groups were 4.8 ± 0.7, 54.2 ± 10.3, 50.1 ± 7.4, and 28.4 ± 4.5, respectively. (4) Western blot showed that the relative expression levels of toll-like receptor-4 (TLR-4) protein in the sham, SAH, NS, and gin groups were 0.29 ± 0.03, 0.87 ± 0.15, 0.65 ± 0.13, and 0.41 ± 0.17, respectively; the relative expression levels of B protein were 0.21 ± 0.04, 0.96 ± 0.14, 0.73 ± 0.18, and 0.30 ± 0.05, respectively. Gin treatment could inhibit TLR-4 and nuclear factor-κB (NF-κB) protein expression.\u0000 \u0000 \u0000 \u0000 Dona tablets may inhibit activation of the NF-κB signaling pathway, and SAH-induced inflammatory response, so as to reduce cerebral vasospasm and EBI.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139850941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-02DOI: 10.4103/2311-8571.395060
G. Zia, Tinku Gupta, Vandana Garg, Mahima Chauhan, Rohit Dutt
The concurrent usage of oral hypoglycemic agents produces side effects, and the herbal medicine Plumbago zeylanica L. (PZ) is being studied to reduce these effects. The antioxidant and antidiabetic effects of PZ roots on streptozotocin-nicotinamide-induced diabetic rats were investigated. Triple maceration method was used for the development of PZ extracts. Standardization of the plant extract and in vitro study was carried out by the physicochemical study, 1, 1-diphenyl-2-picryl-hydrazyl, and α-amylase methods. Animals were divided into seven groups, and the experiment was designed into two parts. Seven groups were taken normal control, diabetic control, hydroalcoholic extract of PZ at different doses (100–200 mg/kg), and metformin (MET, 25 mg/kg) for 21 days. The coadministration of a low dose of PZ (100 mg/kg) and MET (100 mg/kg) was administered orally. Blood glucose level (BGL) was measured continuously for 3 weeks of overnight-fasted animals. Biochemical estimation was assessed by total protein, catalase, nitric oxide, and reduced glutathione content. Histological features of the β-cells were observed through histopathological study. Hydroalcoholic PZ (HAPZ) showed the highest antioxidant and antidiabetic activities, respectively. Oral administration of HAPZ extract (200 mg/ml) for 21 days diminished the BGL significantly from 298 ± 7.05 to 230 ± 5.69 mg/dL, whereas coadministration of HAPZ (100 mg/kg) with MET (10 mg/kg) had also reduced BGL from 286 ± 4.10 to 231 ± 5.75 mg/dL. The best outcomes were obtained when PZ extract (100 mg/kg) was combined with a low dose of MET (10 mg/kg). We can conclude that PZ might be the alternative to synthetic medicines for the management of diabetic disorders.
{"title":"Antidiabetic and Antioxidant Activities of Plumbago zeylanica Roots in Streptozotocin-induced Diabetic Rats","authors":"G. Zia, Tinku Gupta, Vandana Garg, Mahima Chauhan, Rohit Dutt","doi":"10.4103/2311-8571.395060","DOIUrl":"https://doi.org/10.4103/2311-8571.395060","url":null,"abstract":"\u0000 \u0000 \u0000 The concurrent usage of oral hypoglycemic agents produces side effects, and the herbal medicine Plumbago zeylanica L. (PZ) is being studied to reduce these effects. The antioxidant and antidiabetic effects of PZ roots on streptozotocin-nicotinamide-induced diabetic rats were investigated.\u0000 \u0000 \u0000 \u0000 Triple maceration method was used for the development of PZ extracts. Standardization of the plant extract and in vitro study was carried out by the physicochemical study, 1, 1-diphenyl-2-picryl-hydrazyl, and α-amylase methods. Animals were divided into seven groups, and the experiment was designed into two parts. Seven groups were taken normal control, diabetic control, hydroalcoholic extract of PZ at different doses (100–200 mg/kg), and metformin (MET, 25 mg/kg) for 21 days. The coadministration of a low dose of PZ (100 mg/kg) and MET (100 mg/kg) was administered orally. Blood glucose level (BGL) was measured continuously for 3 weeks of overnight-fasted animals. Biochemical estimation was assessed by total protein, catalase, nitric oxide, and reduced glutathione content. Histological features of the β-cells were observed through histopathological study.\u0000 \u0000 \u0000 \u0000 Hydroalcoholic PZ (HAPZ) showed the highest antioxidant and antidiabetic activities, respectively. Oral administration of HAPZ extract (200 mg/ml) for 21 days diminished the BGL significantly from 298 ± 7.05 to 230 ± 5.69 mg/dL, whereas coadministration of HAPZ (100 mg/kg) with MET (10 mg/kg) had also reduced BGL from 286 ± 4.10 to 231 ± 5.75 mg/dL. The best outcomes were obtained when PZ extract (100 mg/kg) was combined with a low dose of MET (10 mg/kg).\u0000 \u0000 \u0000 \u0000 We can conclude that PZ might be the alternative to synthetic medicines for the management of diabetic disorders.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139869185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qu Du Qiang Fei 1 Hao Fang (QDQF1) is a novel Chinese herbal medicine formula used to treat coronavirus disease 2019 (COVID-19). However, the pharmacological mechanisms of action of QDQF1 remain unclear. The objective of this study was to identify the effective ingredients and biological targets of QDQF1 for COVID-19 treatment. The effective ingredients and mechanisms of action of QDQF1 were analyzed by using network pharmacology methods, which included an analysis of the effective ingredients and corresponding targets, COVID-19-related target acquisition, compound-target network analyses, protein-protein interaction network analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses, and molecular docking studies. In total, 288 effective QDQF1 ingredients were identified. We identified 51 core targets from the 148 targets through an overlap between putative QDQF1 targets and COVID-19-related targets. Six key components, including formononetin, kaempferol, luteolin, naringenin, quercetin, and wogonin were identified through component-target network analyses. GO functional enrichment analysis of the core targets revealed 1296 items, while KEGG pathway enrichment analysis identified 148 signaling pathways. Nine central targets (CCL2, CXCL8, IL1B, IL6, MAPK1, MAPK3, MAPK8, STAT3, and TNF) related to the COVID-19 pathway were identified in the KEGG pathway enrichment analysis. Furthermore, molecular docking analysis suggested that the docking scores of the six key components to the nine central targets were better than those to remdesivir. QDQF1 may regulate multiple immune-and inflammation-related targets to inhibit the progression of severe acute respiratory syndrome coronavirus 2, and thus, may be suitable for the treatment of COVID-19.
{"title":"Investigating the Mechanism of Qu Du Qiang Fei 1 Hao Fang Formula against Coronavirus Disease 2019 Based on Network Pharmacology Method","authors":"Yuan-Hua Wang, He-Yang Zhou, Jin-Yun Ma, Gui-Qing Ding, Hua Yu, Yong-Sheng Jin, Xiaodong Cheng","doi":"10.4103/2311-8571.395061","DOIUrl":"https://doi.org/10.4103/2311-8571.395061","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 Qu Du Qiang Fei 1 Hao Fang (QDQF1) is a novel Chinese herbal medicine formula used to treat coronavirus disease 2019 (COVID-19). However, the pharmacological mechanisms of action of QDQF1 remain unclear. The objective of this study was to identify the effective ingredients and biological targets of QDQF1 for COVID-19 treatment.\u0000 \u0000 \u0000 \u0000 The effective ingredients and mechanisms of action of QDQF1 were analyzed by using network pharmacology methods, which included an analysis of the effective ingredients and corresponding targets, COVID-19-related target acquisition, compound-target network analyses, protein-protein interaction network analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses, and molecular docking studies.\u0000 \u0000 \u0000 \u0000 In total, 288 effective QDQF1 ingredients were identified. We identified 51 core targets from the 148 targets through an overlap between putative QDQF1 targets and COVID-19-related targets. Six key components, including formononetin, kaempferol, luteolin, naringenin, quercetin, and wogonin were identified through component-target network analyses. GO functional enrichment analysis of the core targets revealed 1296 items, while KEGG pathway enrichment analysis identified 148 signaling pathways. Nine central targets (CCL2, CXCL8, IL1B, IL6, MAPK1, MAPK3, MAPK8, STAT3, and TNF) related to the COVID-19 pathway were identified in the KEGG pathway enrichment analysis. Furthermore, molecular docking analysis suggested that the docking scores of the six key components to the nine central targets were better than those to remdesivir.\u0000 \u0000 \u0000 \u0000 QDQF1 may regulate multiple immune-and inflammation-related targets to inhibit the progression of severe acute respiratory syndrome coronavirus 2, and thus, may be suitable for the treatment of COVID-19.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139869444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-02DOI: 10.4103/2311-8571.395060
G. Zia, Tinku Gupta, Vandana Garg, Mahima Chauhan, Rohit Dutt
The concurrent usage of oral hypoglycemic agents produces side effects, and the herbal medicine Plumbago zeylanica L. (PZ) is being studied to reduce these effects. The antioxidant and antidiabetic effects of PZ roots on streptozotocin-nicotinamide-induced diabetic rats were investigated. Triple maceration method was used for the development of PZ extracts. Standardization of the plant extract and in vitro study was carried out by the physicochemical study, 1, 1-diphenyl-2-picryl-hydrazyl, and α-amylase methods. Animals were divided into seven groups, and the experiment was designed into two parts. Seven groups were taken normal control, diabetic control, hydroalcoholic extract of PZ at different doses (100–200 mg/kg), and metformin (MET, 25 mg/kg) for 21 days. The coadministration of a low dose of PZ (100 mg/kg) and MET (100 mg/kg) was administered orally. Blood glucose level (BGL) was measured continuously for 3 weeks of overnight-fasted animals. Biochemical estimation was assessed by total protein, catalase, nitric oxide, and reduced glutathione content. Histological features of the β-cells were observed through histopathological study. Hydroalcoholic PZ (HAPZ) showed the highest antioxidant and antidiabetic activities, respectively. Oral administration of HAPZ extract (200 mg/ml) for 21 days diminished the BGL significantly from 298 ± 7.05 to 230 ± 5.69 mg/dL, whereas coadministration of HAPZ (100 mg/kg) with MET (10 mg/kg) had also reduced BGL from 286 ± 4.10 to 231 ± 5.75 mg/dL. The best outcomes were obtained when PZ extract (100 mg/kg) was combined with a low dose of MET (10 mg/kg). We can conclude that PZ might be the alternative to synthetic medicines for the management of diabetic disorders.
{"title":"Antidiabetic and Antioxidant Activities of Plumbago zeylanica Roots in Streptozotocin-induced Diabetic Rats","authors":"G. Zia, Tinku Gupta, Vandana Garg, Mahima Chauhan, Rohit Dutt","doi":"10.4103/2311-8571.395060","DOIUrl":"https://doi.org/10.4103/2311-8571.395060","url":null,"abstract":"\u0000 \u0000 \u0000 The concurrent usage of oral hypoglycemic agents produces side effects, and the herbal medicine Plumbago zeylanica L. (PZ) is being studied to reduce these effects. The antioxidant and antidiabetic effects of PZ roots on streptozotocin-nicotinamide-induced diabetic rats were investigated.\u0000 \u0000 \u0000 \u0000 Triple maceration method was used for the development of PZ extracts. Standardization of the plant extract and in vitro study was carried out by the physicochemical study, 1, 1-diphenyl-2-picryl-hydrazyl, and α-amylase methods. Animals were divided into seven groups, and the experiment was designed into two parts. Seven groups were taken normal control, diabetic control, hydroalcoholic extract of PZ at different doses (100–200 mg/kg), and metformin (MET, 25 mg/kg) for 21 days. The coadministration of a low dose of PZ (100 mg/kg) and MET (100 mg/kg) was administered orally. Blood glucose level (BGL) was measured continuously for 3 weeks of overnight-fasted animals. Biochemical estimation was assessed by total protein, catalase, nitric oxide, and reduced glutathione content. Histological features of the β-cells were observed through histopathological study.\u0000 \u0000 \u0000 \u0000 Hydroalcoholic PZ (HAPZ) showed the highest antioxidant and antidiabetic activities, respectively. Oral administration of HAPZ extract (200 mg/ml) for 21 days diminished the BGL significantly from 298 ± 7.05 to 230 ± 5.69 mg/dL, whereas coadministration of HAPZ (100 mg/kg) with MET (10 mg/kg) had also reduced BGL from 286 ± 4.10 to 231 ± 5.75 mg/dL. The best outcomes were obtained when PZ extract (100 mg/kg) was combined with a low dose of MET (10 mg/kg).\u0000 \u0000 \u0000 \u0000 We can conclude that PZ might be the alternative to synthetic medicines for the management of diabetic disorders.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139808875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qu Du Qiang Fei 1 Hao Fang (QDQF1) is a novel Chinese herbal medicine formula used to treat coronavirus disease 2019 (COVID-19). However, the pharmacological mechanisms of action of QDQF1 remain unclear. The objective of this study was to identify the effective ingredients and biological targets of QDQF1 for COVID-19 treatment. The effective ingredients and mechanisms of action of QDQF1 were analyzed by using network pharmacology methods, which included an analysis of the effective ingredients and corresponding targets, COVID-19-related target acquisition, compound-target network analyses, protein-protein interaction network analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses, and molecular docking studies. In total, 288 effective QDQF1 ingredients were identified. We identified 51 core targets from the 148 targets through an overlap between putative QDQF1 targets and COVID-19-related targets. Six key components, including formononetin, kaempferol, luteolin, naringenin, quercetin, and wogonin were identified through component-target network analyses. GO functional enrichment analysis of the core targets revealed 1296 items, while KEGG pathway enrichment analysis identified 148 signaling pathways. Nine central targets (CCL2, CXCL8, IL1B, IL6, MAPK1, MAPK3, MAPK8, STAT3, and TNF) related to the COVID-19 pathway were identified in the KEGG pathway enrichment analysis. Furthermore, molecular docking analysis suggested that the docking scores of the six key components to the nine central targets were better than those to remdesivir. QDQF1 may regulate multiple immune-and inflammation-related targets to inhibit the progression of severe acute respiratory syndrome coronavirus 2, and thus, may be suitable for the treatment of COVID-19.
{"title":"Investigating the Mechanism of Qu Du Qiang Fei 1 Hao Fang Formula against Coronavirus Disease 2019 Based on Network Pharmacology Method","authors":"Yuan-Hua Wang, He-Yang Zhou, Jin-Yun Ma, Gui-Qing Ding, Hua Yu, Yong-Sheng Jin, Xiaodong Cheng","doi":"10.4103/2311-8571.395061","DOIUrl":"https://doi.org/10.4103/2311-8571.395061","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 Qu Du Qiang Fei 1 Hao Fang (QDQF1) is a novel Chinese herbal medicine formula used to treat coronavirus disease 2019 (COVID-19). However, the pharmacological mechanisms of action of QDQF1 remain unclear. The objective of this study was to identify the effective ingredients and biological targets of QDQF1 for COVID-19 treatment.\u0000 \u0000 \u0000 \u0000 The effective ingredients and mechanisms of action of QDQF1 were analyzed by using network pharmacology methods, which included an analysis of the effective ingredients and corresponding targets, COVID-19-related target acquisition, compound-target network analyses, protein-protein interaction network analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses, and molecular docking studies.\u0000 \u0000 \u0000 \u0000 In total, 288 effective QDQF1 ingredients were identified. We identified 51 core targets from the 148 targets through an overlap between putative QDQF1 targets and COVID-19-related targets. Six key components, including formononetin, kaempferol, luteolin, naringenin, quercetin, and wogonin were identified through component-target network analyses. GO functional enrichment analysis of the core targets revealed 1296 items, while KEGG pathway enrichment analysis identified 148 signaling pathways. Nine central targets (CCL2, CXCL8, IL1B, IL6, MAPK1, MAPK3, MAPK8, STAT3, and TNF) related to the COVID-19 pathway were identified in the KEGG pathway enrichment analysis. Furthermore, molecular docking analysis suggested that the docking scores of the six key components to the nine central targets were better than those to remdesivir.\u0000 \u0000 \u0000 \u0000 QDQF1 may regulate multiple immune-and inflammation-related targets to inhibit the progression of severe acute respiratory syndrome coronavirus 2, and thus, may be suitable for the treatment of COVID-19.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139809642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-16DOI: 10.4103/2311-8571.393789
E. Chan, H. T. Chan, S. Wong
The rich chemical constituents and diverse pharmacological properties of Ramulus Mori (RM) or the twig of Morus alba with evidence supported by clinical trials and patents are reviewed. Known as Sangzhi in Chinese, RM is widely used in traditional Chinese medicine to treat gout, arthritis, and rheumatism. Chemical constituents include flavonoids, benzofurans, alkaloids, stilbenes, chalcones, phenolic acids, and coumarins. Bioactivities of RM include antidiabetic, anti-obesity, anti-inflammatory, antityrosinase, neuroprotective, antioxidant, hepatoprotective, cytoprotective, renoprotective, antihyperuricemic, analgesic, antifungal, DNA protective, anticancer, gastroprotective, cardioprotective, anti-hair aging, anti-arthritic, and antiplatelet aggregation properties. The most active compounds from RM are mulberrin, oxyresveratrol, and mulberroside A. All four clinical trials on RM are focused on the treatment of diabetes. The patents entail using RM extracts as cosmetics for skin whitening and as agents for hypoglycemia. Topics for further research on RM are suggested.
{"title":"An Overview of Chemical Constituents, Medicinal Properties, Clinical Trials, and Patents of Twigs of Morus alba (Ramulus Mori)","authors":"E. Chan, H. T. Chan, S. Wong","doi":"10.4103/2311-8571.393789","DOIUrl":"https://doi.org/10.4103/2311-8571.393789","url":null,"abstract":"\u0000 The rich chemical constituents and diverse pharmacological properties of Ramulus Mori (RM) or the twig of Morus alba with evidence supported by clinical trials and patents are reviewed. Known as Sangzhi in Chinese, RM is widely used in traditional Chinese medicine to treat gout, arthritis, and rheumatism. Chemical constituents include flavonoids, benzofurans, alkaloids, stilbenes, chalcones, phenolic acids, and coumarins. Bioactivities of RM include antidiabetic, anti-obesity, anti-inflammatory, antityrosinase, neuroprotective, antioxidant, hepatoprotective, cytoprotective, renoprotective, antihyperuricemic, analgesic, antifungal, DNA protective, anticancer, gastroprotective, cardioprotective, anti-hair aging, anti-arthritic, and antiplatelet aggregation properties. The most active compounds from RM are mulberrin, oxyresveratrol, and mulberroside A. All four clinical trials on RM are focused on the treatment of diabetes. The patents entail using RM extracts as cosmetics for skin whitening and as agents for hypoglycemia. Topics for further research on RM are suggested.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139528088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-12DOI: 10.4103/2311-8571.393751
Haixia Shang, Yi Fang, Bin Guan, Jian-Hua Guan, Jun Peng, Jin-yan Zhao, Jiumao Lin
The aim of this study was to explore the effects of Babao dan (BBD), a traditional Chinese medicine, on gastric cancer (GC) progression in vivo. A subcutaneous xenograft mouse model of GC was established using MGC80-3 cells. The terminal deoxynucleotidyl transferase-mediated dUTP: 2’-deoxyuridine 5’-triphosphate -biotin nick-end labeling method was adopted to detect cell apoptosis in vivo. The expression levels of proteins associated with proliferation, apoptosis, and angiogenesis were measured by immunohistochemical staining or western blotting (WB). The activation and protein levels of p-c-Jun N-terminal kinase (JNK), p-p38, p-extracellular-regulated kinase 1/2, p-nuclear factor-κB (NF-κB), and p-STAT3 were examined by Bio-plex and WB. BBD significantly inhibited tumor growth in GC mouse models with no adverse effect on body weight or organ function. It was also found that BBD significantly suppressed the proliferation of GC tumor cells, induced the apoptosis of tumor cells, and inhibited angiogenesis through inactivating with mitogen-activated protein kinase, NF-κB, and STAT3 pathways. BBD exerts suppressive effects on GC tumor growth by regulating multiple pathways in vivo, which may provide a novel treatment option for GC therapy.
{"title":"Babao Dan Inhibits Gastric Cancer Progression in vivo through Multiple Signaling Pathways","authors":"Haixia Shang, Yi Fang, Bin Guan, Jian-Hua Guan, Jun Peng, Jin-yan Zhao, Jiumao Lin","doi":"10.4103/2311-8571.393751","DOIUrl":"https://doi.org/10.4103/2311-8571.393751","url":null,"abstract":"\u0000 \u0000 \u0000 The aim of this study was to explore the effects of Babao dan (BBD), a traditional Chinese medicine, on gastric cancer (GC) progression in vivo.\u0000 \u0000 \u0000 \u0000 A subcutaneous xenograft mouse model of GC was established using MGC80-3 cells. The terminal deoxynucleotidyl transferase-mediated dUTP: 2’-deoxyuridine 5’-triphosphate -biotin nick-end labeling method was adopted to detect cell apoptosis in vivo. The expression levels of proteins associated with proliferation, apoptosis, and angiogenesis were measured by immunohistochemical staining or western blotting (WB). The activation and protein levels of p-c-Jun N-terminal kinase (JNK), p-p38, p-extracellular-regulated kinase 1/2, p-nuclear factor-κB (NF-κB), and p-STAT3 were examined by Bio-plex and WB.\u0000 \u0000 \u0000 \u0000 BBD significantly inhibited tumor growth in GC mouse models with no adverse effect on body weight or organ function. It was also found that BBD significantly suppressed the proliferation of GC tumor cells, induced the apoptosis of tumor cells, and inhibited angiogenesis through inactivating with mitogen-activated protein kinase, NF-κB, and STAT3 pathways.\u0000 \u0000 \u0000 \u0000 BBD exerts suppressive effects on GC tumor growth by regulating multiple pathways in vivo, which may provide a novel treatment option for GC therapy.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139624357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.4103/2311-8571.393752
Elham Najafi, Bahareh Babaei Hoolari, Akbar Karimi, Aliasghar Pilehvarian
Dracaena cinnabari is considered a rich source of phytochemicals used widely in traditional medicine. In the present study, the effect of D. cinnabari hydraulic extract on the reproductive system of female rats was investigated. The samples were randomly divided into four groups (six samples in each group), including three treatment groups and one control group, and all samples were kept at the same conditions. Hydraulic extract of D. cinnabari and injected intraperitoneally daily for 10 days, while physiological serum was used for injection in to the control group. After 10 days of injection, estrogen and progesterone levels were measured by enzyme immunoassay technique. After dissection, the ovaries and uterine tissues were isolated for histological examination, and tissue changes were carefully examined. The results revealed that the levels of estrogen and progesterone in experimental Groups 2 and 3 had a significant increase (P < 0.001). Regarding tissue changes, a significant increase was observed in epithelial thickness (P < 0.001), number of corpus luteum (P < 0.01), and Graafian follicle (P < 0.01) in doses of 100 and 150 mg/kg. Based on the results, it seems that D. cinnabari extract has an effect on the ovarian follicles.
{"title":"Examining the Effect of Hydroalcoholic Extract of Dracaena cinnabari on Sex Hormones and Ovarian and Uterine Tissues of Rats","authors":"Elham Najafi, Bahareh Babaei Hoolari, Akbar Karimi, Aliasghar Pilehvarian","doi":"10.4103/2311-8571.393752","DOIUrl":"https://doi.org/10.4103/2311-8571.393752","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 Dracaena cinnabari is considered a rich source of phytochemicals used widely in traditional medicine. In the present study, the effect of D. cinnabari hydraulic extract on the reproductive system of female rats was investigated.\u0000 \u0000 \u0000 \u0000 The samples were randomly divided into four groups (six samples in each group), including three treatment groups and one control group, and all samples were kept at the same conditions. Hydraulic extract of D. cinnabari and injected intraperitoneally daily for 10 days, while physiological serum was used for injection in to the control group. After 10 days of injection, estrogen and progesterone levels were measured by enzyme immunoassay technique. After dissection, the ovaries and uterine tissues were isolated for histological examination, and tissue changes were carefully examined.\u0000 \u0000 \u0000 \u0000 The results revealed that the levels of estrogen and progesterone in experimental Groups 2 and 3 had a significant increase (P < 0.001). Regarding tissue changes, a significant increase was observed in epithelial thickness (P < 0.001), number of corpus luteum (P < 0.01), and Graafian follicle (P < 0.01) in doses of 100 and 150 mg/kg.\u0000 \u0000 \u0000 \u0000 Based on the results, it seems that D. cinnabari extract has an effect on the ovarian follicles.\u0000","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: Call on people to treat the causes of physical diseases is to take into account the causes of psychological factors as well as external causes. Materials and Methods: The analysis was conducted by combining the classical medical books of Tibetan medicine, as well as the traditional culture and living habits of Tibetan people, with modern research results. Results: Many of the theories mentioned in Tibetan medicine related to mind-body medicine have been confirmed by modern research. Mental and physical treatment related to psychosomatic diseases should be administered simultaneously. First, when a person is healthy, he should cultivate his mind to build a solid psychological defense against diseases. Developing both the mind and body contributes to creating a stable physical protection barrier against diseases. When a person is ill, he should realign his mind and help his body adjust and promote its early recovery with the help of medications. Conclusions: Treating related psychosomatic diseases should treat the mind and body simultaneously. And the mind and body should be cultivated before diseases to reinforce the psychological and physical defense against diseases.
{"title":"The psychosomatic thought of tibetan medicine and its treatment methods","authors":"Zijuan Zhang, Xin Li, Yasong Cheng, Amatti Jorigori, Dongzhu Renqing, Liwen Pan, Meng Mao, Xiao-Qiao Ren, Huizhen Zhao","doi":"10.4103/2311-8571.373598","DOIUrl":"https://doi.org/10.4103/2311-8571.373598","url":null,"abstract":"Objectives: Call on people to treat the causes of physical diseases is to take into account the causes of psychological factors as well as external causes. Materials and Methods: The analysis was conducted by combining the classical medical books of Tibetan medicine, as well as the traditional culture and living habits of Tibetan people, with modern research results. Results: Many of the theories mentioned in Tibetan medicine related to mind-body medicine have been confirmed by modern research. Mental and physical treatment related to psychosomatic diseases should be administered simultaneously. First, when a person is healthy, he should cultivate his mind to build a solid psychological defense against diseases. Developing both the mind and body contributes to creating a stable physical protection barrier against diseases. When a person is ill, he should realign his mind and help his body adjust and promote its early recovery with the help of medications. Conclusions: Treating related psychosomatic diseases should treat the mind and body simultaneously. And the mind and body should be cultivated before diseases to reinforce the psychological and physical defense against diseases.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":4.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45603556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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