Veterinary Sciences最新文献

Assisted reproductive technologies (ART) use has increased over the past decades. However, reports concerning ART's low efficiency continue to emerge, citing causes related to lower embryo quality and pregnancy rates compared to their in vivo counterparts. One of the setbacks of ART is oxidative stress, which can impair embryo developmental rates. Mitochondrial redox and energetic homeostasis determine both cell survival and death, so mitochondria are a key target for therapeutic intervention strategies. In the present work, our objective was to improve the quality of viable embryos by adding new mitochondria-targeted antioxidants in the embryo culture media to reduce oxidative stress. Two naturally derived antioxidants synthesized by our team, AntiOxBEN₂ and AntiOxCIN₄, based on hydroxybenzoic and hydroxycinnamic scaffolds, respectively, were studied in two different experimental protocols (here called experiments). The first experiment investigated the effects of the antioxidants on embryo development to determine their optimal concentrations. The first assay of the first experiment focused on the effects of AntiOxCIN₄ at concentrations of 1, 2.5, and 10 μM, while the second assay focused on the effects of AntiOxBEN₂ at the same concentrations. A control group without supplementation was run simultaneously. The second experiment aimed to compare the best concentrations of these antioxidant molecules in the embryo culture media and their effect on embryos' resistance to vitrification/warming. In each experiment, the embryos were morphologically evaluated, and the total and viable cell numbers were examined. Reactive oxygen species (ROS) and mitochondrial polarization were also evaluated using specific fluorescent dyes. In experiment 1, an increased embryo quality was identified by using 2.5 μM AntiOxCIN₄ (p = 0.03) and 2.5 μM AntiOxBEN₂ (p = 0.001). Moreover, blastocysts supplemented with 2.5 μM AntiOxCIN₄ had higher viability (p = 0.008), while those supplemented with 2.5 μM AntiOxBEN₂ presented a greater total cell number (p = 0.01). An improvement in embryo cryosurvival following the supplementation during the culture process with either antioxidant was identified in experiment 2, with superior expansion scores after vitrification/warming and culture (2.5 μM AntiOxCIN₄, p = 0.056 and 2.5 μM AntiOxBEN₂, p = 0.059). In conclusion, both AntiOxCIN₄ and AntiOxBEN₂ had a beneficial effect on embryo development and cryosurvival, suggesting a potential intervention to reduce oxidative stress in assisted reproductive technologies.

This study investigated the effects of dietary black cumin seed meal (BCSM) supplementation on growth performance, carcass traits, intestinal histomorphology, cecal microbiota, meat quality, and breast meat malondialdehyde (MDA) levels in Japanese quail. A total of 200 one-week-old quail were randomly allocated to four dietary treatments containing 0, 5, 10, or 20 g/kg BCSM for a 35-day experimental period, with five replicates per treatment. Dietary BCSM supplementation did not significantly affect body weight, body weight gain, feed intake, or conversion ratio (p > 0.05). However, carcass weight was significantly increased in birds fed 20 g/kg BCSM (p < 0.05), while carcass yield and relative internal organ weight remained unchanged. Intestinal histomorphology was markedly influenced by dietary treatments (p < 0.05), with improved villus height and villus height-to-crypt depth ratio in the jejunum and ileum of BCSM-fed birds. In addition, cecal microbiota analysis revealed a dose-dependent increase in Lactobacillus spp. (p < 0.05), whereas Escherichia coli counts were numerically reduced but not statistically affected (p > 0.05). Meat quality evaluation showed that BCSM supplementation significantly increased breast meat lightness (L*) and water-holding capacity and reduced post-slaughter pH values (p < 0.05). Lipid oxidation, as assessed by malondialdehyde (MDA) concentrations, was observed to be significantly lower in breast meat during refrigerated storage, suggesting a potential improvement in oxidative stability and a possible contribution to extended shelf life (p < 0.05). In conclusion, dietary inclusion of black cumin seed meal, particularly at 20 g/kg, positively modulated intestinal health, cecal microbiota composition, and meat oxidative stability without compromising growth performance. Owing to its high nutritional value and rich bioactive compound profile, BCSM can be considered a functional and sustainable feed ingredient for quail nutrition.

Background: Poultry-associated Enterococcus spp. are widespread commensals but may serve as One Health indicators when virulence-associated determinants and antimicrobial resistance co-occur. We characterized paired phenotypic and genomic profiles to delineate species-stratified virulome and resistome patterns. Methods: Isolates originated from a previously established poultry collection with MIC testing. Genotype-phenotype analyses were restricted to the whole-genome sequenced subset (n = 31). The acquired antimicrobial resistance genes were identified using the Comprehensive Antibiotic Resistance Database (CARD), and virulence-associated determinants were screened using the Virulence Factors Database (VFDB). Results were summarized as isolate-level presence/absence matrices and integrated with MIC-derived susceptible/intermediate/resistant categories. Results: The WGS subset comprised E. faecalis (n = 23) and E. faecium (n = 8) with diverse sequence types. Virulome architecture was strongly species-dependent: E. faecalis carried a broad repertoire of adhesion/biofilm-associated determinants, whereas E. faecium showed a limited set of high-confidence virulence-associated hits. Acquired resistance determinants were common across isolates, and resistome profiles displayed structured co-occurrence. Integrated analyses suggested only a modest overall association between virulence-gene burden and acquired resistome size, largely driven by species-level differences. Genotype-phenotype concordance was class-dependent, with incomplete alignment in several antimicrobial classes, consistent with mechanisms beyond the screened acquired gene set. The acquired resistance determinants detected in the WGS subset predominantly mapped to antimicrobial classes commonly used in food-producing animals (e.g., tetracyclines, macrolides, lincosamides, aminoglycosides, and phenicols), supporting interpretation in the context of production-associated antimicrobial selection rather than implying last-line clinical resistance by default. Conclusions: Poultry-derived enterococci may combine genetic features compatible with persistence/colonization and acquired antimicrobial resistance, with co-occurrence patterns shaped primarily by species/lineage background. These findings support risk-stratified One Health surveillance and targeted functional and mechanism-focused follow-up. This integrated virulome-resistome view highlights species-specific risk signatures in poultry-associated Enterococcus and identifies discordant high-level phenotypes that merit targeted mechanistic follow-up.

Feline heartworm disease, caused by Dirofilaria immitis, is often underdiagnosed, particularly during its larval stage, known as Heartworm-Associated Respiratory Disease, (HARD). This study aimed to quantify CT-detectable pulmonary changes in naturally infected cats. Computed tomography (CT) was performed in 38 cats: Group A (n = 30, symptomatic seropositive) and Group B (n = 8, asymptomatic seronegative). Bronchial and pulmonary artery diameters were indexed to the sixth thoracic vertebral body (T6) to calculate bronchial-to-vertebral body (B/VB) and pulmonary artery-to-vertebral body (A/VB) ratios across all lung lobes. Group A cats showed significantly higher B/VB ratios in the left cranial and right middle lobes compared with Group B (p < 0.05), with increases of 42.0% and 47.5%, respectively. Conversely, A/VB ratios did not differ significantly between groups (p > 0.05), indicating the absence of relevant vascular remodelling. Intra-operator and inter-observer reliability were excellent (intraclass correlation coefficients > 0.85). These findings suggest that bronchial dilation in the absence of arterial enlargement represents a key tomographic feature of larval D. immitis infection. Quantitative CT ratios, particularly the B/VB ratio, provide objective imaging markers for the diagnosis of HARD in clinical practice, and may assist in differentiating this condition from other feline respiratory diseases in endemic areas.

Artificial intelligence (AI), including machine learning (ML) and deep learning (DL), is rapidly transforming clinical veterinary practice by enhancing diagnostics, disease surveillance and decision support processes across animal health domains. The safe and effective clinical deployment of these technologies, however, depends critically on the preparedness of the veterinary workforce, positioning veterinary education as a strategic enabler of translational adoption. This narrative review examines the integration of AI within veterinary education as a foundational step toward its responsible application in clinical practice. We synthesize current evidence on AI-driven tools relevant to veterinary curricula, including generative and multimodal large language models, intelligent tutoring systems, virtual and augmented reality platforms and AI-based decision support tools applied to imaging, epidemiology, parasitology, food safety and animal health. Particular attention is given to how the structured educational use of AI mirrors real-world clinical workflows and supports the development of competencies essential for clinical translation, such as data interpretation, uncertainty management, ethical reasoning and professional accountability. The review further addresses ethical, regulatory and cognitive considerations associated with AI adoption, including algorithmic bias, data privacy, equity of access and the risks of overreliance, emphasizing their direct implications for diagnostic reliability and animal welfare. By framing veterinary education as a controlled and reflective environment for AI engagement, this article highlights how pedagogically grounded training can facilitate safer clinical deployment, foster interdisciplinary collaboration and align technological innovation with professional standards in veterinary medicine.

Although the gut microbiome constitutes a key component of vertebrate physiology, comparative baseline data for companion birds, particularly parrots, remain limited. Therefore, this study profiled the fecal gut microbiota of 31 privately owned companion parrots representing 14 psittacine species maintained in indoor household environments for >6 months. Amplicons targeting the V3-V4 region of the 16S rRNA gene were sequenced, denoised into amplicon sequence variants using QIIME 2 with DADA2, and taxonomically assigned against the SILVA v132 database. Community composition was broadly dominated by Firmicutes and Proteobacteria, with recurrent detection of Lactobacillus across most samples, consistent with a potential core component of the gut microbiome of captive parrots. Taken together, this study provides an exploratory comparative snapshot of fecal gut microbiota across diverse companion parrot species and establishes baseline reference data for future research linking diet, husbandry practices, and health to microbiome variation, including longitudinal and wild-captive comparative investigations.

With the rapid development of the livestock and poultry industry, the availability of feed resources in China has become a critical limiting factor, posing a significant challenge to the sustainable growth of animal husbandry. Black soldier fly (BSF) larvae are rich in protein, lipids, minerals, and trace elements and possess an essential amino acid profile comparable to that of fishmeal and soybean meal, which makes them a promising novel protein source for feed. This study aimed to investigate the effects of dietary BSF larvae protein supplementation on the growth performance, egg production, as well as meat and egg quality, blood biochemical parameters, and cecal microbiota diversity of Yunshang countryside chickens. The results showed that the inclusion of BSF larvae protein in the diet significantly reduced the feed-to-egg ratio and enhanced egg quality. Dietary supplementation with BSF larvae protein also effectively increased the abundance of dominant bacterial phyla and genera in the cecum, with the optimal inclusion level identified as 7.5%. Overall, the results demonstrate that BSF larvae can serve as a high-quality protein source in poultry production, thereby providing a scientific reference for the development and application of new feed resources and offering a theoretical basis for the utilization of BSF larvae as an alternative protein ingredient.

Veterinary metabolomics has redefined bile acids (BAs) from simple digestive surfactants to systemic endocrine signals within a microbial-host metabolic axis. This review aims to evaluate how BA dysmetabolism acts as a central pathogenic factor in canine and feline disease. We analyze the BA pool's integrity, which depends on a specialized functional guild, primarily Peptacetobacter hiranonis, responsible for 7α-dehydroxylation. We delineate two principal pathological profiles: (1) microbial collapse, characterized by secondary bile acid (SBA) depletion and compromised farnesoid X receptor (FXR) and Takeda G protein-coupled receptor 5 (TGR5) signaling, which exacerbates inflammation in chronic enteropathy (CE), protein-losing enteropathy (PLE), and exocrine pancreatic insufficiency (EPI); and (2) hepato-biliary spillover, wherein host-induced dysfunction results in primary bile acid (PBA) excess. Recent data have linked these disruptions to skeletal health, feline renal fibrosis, cardiac remodeling in myxomatous mitral valve disease (MMVD), and neuroinflammation in epilepsy and hepatic encephalopathy. The discovery of microbially conjugated bile acids (MCBAs) and microbial extracellular vesicles (MEVs) reveals highly specific, vesicle-mediated communication pathways impacting systemic health. Diagnostic protocols should prioritize functional profiling, including the dysbiosis index (DI), serum conjugated BA analysis, and SBA/PBA ratios. Clinical management is moving beyond empirical fecal microbiota transplantation (FMT), towards precision synthetic microbial consortia (SynComs), neuroprotective BAs like tauroursodeoxycholic acid (TUDCA), and molecular postbiotics to restore the collaborative metabolome.

Background: Canine pyoderma is a common bacterial skin disease that can be classified as superficial or deep and is associated with inflammatory processes. Systemic inflammatory biomarkers such as procalcitonin (PCT), interleukin-8 (IL-8), and beta-defensin-2 (Defb2) may reflect immune activation; however, their diagnostic and clinical relevance in canine pyoderma remains unclear. Materials and Methods: Serum concentrations of PCT, IL-8, and Defb2 were measured using enzyme-linked immunosorbent assays (ELISA) in healthy control dogs (group 1, n = 40), dogs with superficial pyoderma (group 2a, n = 16), and dogs with deep pyoderma (group 3a, n = 7). A subset of dogs with superficial pyoderma (group 2b, n = 12) was re-evaluated after clinical remission. Biomarker concentrations were statistically compared between groups and over time. Results: Dogs with superficial and deep pyoderma exhibited significantly higher serum concentrations of PCT and IL-8 compared to healthy controls, whereas Defb2 concentrations were significantly reduced in both disease groups. No statistically significant differences were detected between superficial and deep pyoderma for any of the biomarkers, although IL-8 showed a trend toward higher concentrations in dogs with deep pyoderma (p = 0.07). Follow-up examinations after clinical improvement revealed no significant changes in biomarker concentrations. Conclusions: Canine pyoderma is associated with measurable systemic inflammatory alterations, characterized by increased serum concentrations of PCT and IL-8 and decreased Defb2 levels, irrespective of disease depth. The lack of biomarker normalization following clinical remission suggests that systemic inflammatory responses may persist beyond visible clinical healing. While these biomarkers may provide complementary information on inflammatory activity, their utility for monitoring treatment response appears limited.

Bovine viral diarrhea virus (BVDV) is a critical pathogen affecting the global cattle industry, causing severe economic losses primarily through persistent infection, immunosuppression, and reproductive failure. The virus exhibits substantial genetic diversity, with marked geographic variation in circulating subtypes, which complicates effective disease control. BVDV evades host immune responses by suppressing type I interferon signaling, impairing neutrophil function, and reprogramming host cellular metabolism, ultimately leading to the generation of persistently infected (PI) animals that serve as the principal reservoir for viral transmission. Current prevention and control strategies rely mainly on the identification and elimination of PI animals in combination with vaccination. However, conventional vaccines, including inactivated vaccines (IVs) and modified live vaccines (MLVs), have notable limitations, such as suboptimal subtype matching, interference by maternal antibodies, and safety concerns associated with MLV use in pregnant cattle. Emerging vaccine platforms, including mRNA vaccines, subunit vaccines, and multi-epitope vaccines, offer promising alternatives owing to their improved safety profiles, rapid design and production, and potential to elicit broad and robust immune responses. Future BVDV vaccine development should integrate artificial intelligence-driven design strategies with high-throughput sequencing and molecular epidemiological surveillance to enable the rational development of multivalent and multi-epitope vaccines. In addition, coordinated implementation of strain monitoring, PI animal clearance, and enhanced biosecurity practices will be essential for establishing a comprehensive and sustainable BVDV prevention and control framework.