Veterinary Sciences最新文献

The lesser mealworm (Alphitobius diaperinus) is a significant pest in poultry facilities, transmitting pathogens such as Salmonella and causing structural damage in poultry operations. Challenges such as insecticide resistance and environmental concerns associated with the widespread use of traditional insecticides underscore the need for sustainable alternatives. This review examines biological and sustainable management strategies for managing the lesser mealworm, including entomopathogenic fungi, bacteria, nematodes, plant essential oils-extracts, pheromones, and diatomaceous earth. These eco-friendly approaches highlight fungi and nematodes for their effectiveness in larval control and essential oils for their insecticidal and repellent properties. The application of pheromones and diatomaceous earth further complements other methods within integrated pest management (IPM) systems. Integrating these biological tools into IPM offers a sustainable and effective solution for reducing A. diaperinus populations, addressing both health and environmental concerns.

This study aimed to analyze the effect of reducing litter size variability on growth rate, carcass traits, and meat quality in rabbits. Two divergent lines selected by litter size variability were compared: a line selected to reduce litter size variability (HO) and one selected to increase litter size variability (HE). All animals were fed the same commercial diet and kept under identical rearing conditions. The HO line had lower live weight at weaning compared to the HE line (p ≤ 0.05), but the growth rate was higher in the HO line than in the HE line (p ≤ 0.05). Carcass weight and composition were similar between the lines. No differences in meat pH at 45 min and 24 h post-mortem were found between lines. However, the HO line had a higher lightness (L*) value at 45 min post-mortem than the HE line (p ≤ 0.05). The HO line showed higher Ca concentrations than the HE line (p ≤ 0.05). The HO line also had higher protein content and lower saturated fatty acid content than the HE line (p ≤ 0.05). No differences in fat oxidation were observed among the lines. In conclusion, the selection to reduce litter size variability did not negatively impact growth rate and had a favorable effect on protein content, as well as a reduction in saturated fatty acid content in carcass.

Veterinary anaesthesia requires theoretical knowledge and quick decision-making skills. Traditional education may not adequately prepare students, while simulation-based learning enhances engagement and skill development. This study evaluates the effectiveness of a Twine-based web system in improving experiential learning, engagement, knowledge retention, and decision-making skills in veterinary anaesthesia students. Five interactive clinical cases were developed using Twine, simulating realistic anaesthesia scenarios with decision points and gamified elements, such as scoring systems and resource management. These modules were accessible on various devices via the web. Following a workshop for second- to fourth-year students of the Degree in Veterinary Sciences, an anonymous survey assessed the module's effectiveness. Quantitative data were analysed descriptively, while qualitative feedback was processed through a hybrid AI-human thematic analysis. Out of 849 invited students, 367 responded (42% response rate). Feedback was highly positive; 90.8% found it effective for training, and 97.0% agreed it improved knowledge. User-friendliness was rated as "easy" or "very easy" by 94.6%. Regarding overall satisfaction, 96.7% of students described the workshop as "good" or "excellent". Some participants suggested improvements in mobile device compatibility and the need for additional resources to understand the concepts better. Twine's interactive format fosters experiential learning while reducing reliance on live animals, aligning with modern ethical standards. Its accessibility via web and translation-enabled browsers enhances its reach. Future research should examine Twine's impact on clinical skills retention and adaptability in various educational contexts, providing a flexible approach to veterinary anaesthesia education through gamified learning.

The study of the function of the right ventricle (RV) is a topic of great interest in the scientific community, and some studies have evaluated parameters of the right ventricular systolic function and have correlated them to various RV pathologies and possible clinical findings. Less information was obtained on the right ventricular diastolic function. In veterinary medicine, the characteristics of the restrictive RV and the presence of end-diastolic forward flow (EDFF) in patients with pulmonary stenosis have not yet been described. This type of flow is an antegrade flow that is observed with the Doppler study of the pulmonary artery and occurs at the end of the diastolic phase. Pulmonary valve stenosis (PVS) is one of the most common congenital heart diseases in dogs. The echocardiographic parameter that is mainly taken as a reference for evaluating the severity of the disease is the peak gradient of the pulmonary antegrade flow. For our retrospective study, we consider a total of 164 dogs with PVS divided into 149 dogs with type A and 15 type B, seen at a reference veterinary clinic from 2020 to 2024. All these dogs underwent echocardiographic examinations to observe the morphological characteristics of the PVS and consider the presence or absence of restrictive RV with EDFF. An independent sample t-test analysis revealed that the severity of PVS is greater in dogs with EDFF, both in the group of patients with type A stenosis and in patients with type B stenosis. This study suggests that RV restrictive physiology is common in dogs with severe PVS.

The isolation of multidrug-resistant (MDR) bacteria from the urinary tracts of pets is increasingly common, particularly in animals with concurrent health conditions. Pseudomonas aeruginosa (PA) is one of the most significant antimicrobial-resistant bacteria affecting cats and dogs within the European Union (EU). This study aims to review the prevalence and antimicrobial resistance patterns of PA isolated from urine samples of small animals globally. This pathogen is known for its opportunistic infections and is a significant concern in veterinary medicine due to its inherent resistance to multiple antibiotics and its ability to acquire additional resistance mechanisms. This review seeks to enhance educational initiatives regarding the management of emerging MDR bacteria.

The early detection of lameness in elephants is essential for effective treatment and welfare enhancement, but subtle gait abnormalities are often difficult to identify visually. This study aimed to evaluate vertical movement symmetry in Asian elephants using cross-correlation analysis of data from inertial measurement units (IMUs). Six elephants were assessed, including individuals with normal gait and one exhibiting an abnormal gait. IMU sensors were attached to the proximal and distal segments of the forelimbs and hindlimbs to record vertical movements during straight-line walking. Cross-correlation coefficients were calculated to quantify the symmetry between the left and right limbs, providing an objective measure of gait patterns. This method provided an objective assessment of gait patterns and demonstrated potential in detecting lameness in elephants. This approach could facilitate the early identification of gait abnormalities, enabling timely interventions and potentially improving treatment outcomes and the welfare of captive elephant populations. Further studies involving a larger number of elephants with confirmed gait abnormalities are necessary to validate the robustness and reliability of this approach.

As global income levels and population increase, meat consumption and production are rising, raising concerns about climate change, animal welfare, and environmental sustainability. Offal offers a sustainable, nutrient-rich, and economical alternative to muscle meat, providing proteins, vitamins, and minerals. This study was triggered by the scarcity of information on the microbiological quality of edible bovine offal, which is often associated with poor hygienic conditions during handling and processing at slaughterhouse. This study assessed the superficial microbiota present on cattle liver and tongue in a vertical slaughterhouse across various stages of the procurement process and on days with different slaughter volumes, with three sampling points: immediately after post-mortem inspection (Point 1); after the arrival of the viscera in the preparation room (Point 2); and in the refrigeration chamber at 3 °C (Point 3). Samples were analysed according to ISO methods for enumeration of mesophilic aerobic bacteria, Enterobacteriaceae, generic Escherichia coli, presumptive Escherichia coli O157, and Staphylococcus aureus and detection of presumptive Escherichia coli O157, Staphylococcus aureus, and Salmonella spp. Microbiological counts were within the acceptability values stipulated in the amendment of Regulation EC 1441/2007 to Regulation EC 2073/2005 for cattle carcasses. In general, both viscera showed higher microbial counts on days with higher slaughter volumes, with the exception of Enterobacteriaceae and Staphylococcus counts on the tongue. There was also an increase in microorganism levels across the offal processing stages. Out of 144 samples, 20.83% were contaminated with presumptive Escherichia coli O157, 3.47% Salmonella spp., and 25% S. aureus. Therefore, although 20.83% of the samples tested were presumptive for E. coli O157, it is important to note that a considerable number of these presumptive positives may in fact have been negative after confirmation by molecular methods. However, the presence of presumptive E. coli O157 at high levels is one reason that the prevention of offal contamination requires more stringent measures. Preventing offal contamination requires stricter measures during slaughter procedures to ensure safety, reduce food losses, and enhance sustainability in meat production.

In order to obtain high-performing yeast strains from ruminants, it is necessary to select them from species such as beef cattle, dairy cows, goats, and buffalo. A total of 91 isolated yeasts were collected using the standard methods of microbial culture on agar medium followed by streaking on a plate at least three times until pure yeast colonies were formed. The API 20C AUX Kit and sequencing of the D1/D2 domain of the 26S rRNA gene were used to identify the genera Candida spp., namely, C. glabrata (99% identification), C. tropicallis (99%), C. rugosa (98%), and Issatchenkia orientalis (99%). A total of 12 yeast strains (Dc4, 14, 18; Be1, 2, 7; Bu3, 4, 7; and Go10, 16, 19) were chosen for further analyses. The performance criteria included the ability to tolerate pH values between 3.5 and 7.5, total volatile fatty acids (TVFAs, 0, 0.25, 0.5, 1, 2, and 4% of broth medium), anaerobic growth rate, and in vitro gas production efficiency. First, when all strains were grown at pH values between 3.5 and 7.5, Bu3 and Dc18 performed better than the other strains. Second, at a ruminal pH of 6.5 and a TVFA concentration of between 2 and 4% of the broth medium, strain Bu3 was more resistant than the other strains. Under anaerobic conditions, all strains experienced a decline in viable cell counts when compared with those under aerobic conditions. However, compared to strains Dc14, Be1, Be2, Be7, and Bu3, strain Dc18 exhibited more viable cells under anaerobic conditions in broth medium. The response of strain Dc18 did not differ from those of strains Dc4, Bu4, Bu7, or G16. Strains Be7, Bu3, and Dc18 were used for an in vitro fermentation experiment involving incubation for 2, 4, 6, 8, 10, 12, 24, 36, 48, and 72 h. Three ruminal cannulated dairy cows were used as donors of ruminal fluid. The treatments were run in triplicate. The addition of yeast culture had no effect on gas kinetics, gas accumulation, or the ratio of acetic acid and propionic acid, but led to significantly greater butyric acid concentrations at 24 h of incubation. In conclusion, strain Dc18 isolated from dairy cows is suitable for future studies of probiotic yeast development.

The liver, as the largest metabolic and detoxification organ in mammals, metabolizes approximately 80-90% of drugs. However, drug-induced liver injury (DILI) is common and driven by factors such as individual variability, differences in liver metabolism, and improper drug use. Mesenchymal stem cells (MSCs), with their self-renewal and multipotent differentiation capabilities, offer therapeutic potential, but face challenges such as limited proliferation and increased apoptosis during in vitro expansion. Although MSCs exhibit low immunogenicity, they are often cleared by the host immune system, which limits their survival and engraftment. Glutathione peroxidase 3 (GPX3) is a key antioxidant enzyme that reduces reactive oxygen species (ROS), protecting cells from oxidative damage. CD47, also known as integrin-associated protein (IAP), helps cells evade immune clearance by binding to signal regulatory protein alpha (SIRPα) on the immune cells. Here, we used an acetaminophen (APAP)-induced DILI mouse model to evaluate the therapeutic efficacy of intravenously infused MSCs overexpressing GPX3 and CD47. Compared to unmodified MSCs, modified MSCs showed improved survival, reduced liver inflammation, and alleviated oxidative damage, offering enhanced protection against APAP-induced DILI.

This study aimed to determine the impact of supplementations of copper, vitamins A and D (ADCU), and a bovine colostrum extract (BC) on the micronutrient status, antioxidant status, and intestinal microbiota of piglets until the post-weaning period. Twenty-three sows were fed conventional gestation and lactation diets, and twenty-four sows were fed conventional diets supplemented with ADCU. For each litter, all piglets received one of four treatments during lactation: no supplementation; ADCU; BC; and ADCU + BC. Within each litter, one low (LW) and one high birth weight (HW) piglet were euthanized before and after weaning to collect liver and intestinal samples. Serum vitamin D, liver retinol, and liver Cu were greater in ADCU piglets (p < 0.01), mostly before weaning. After weaning, liver Cu decreased markedly with a drop of 75% in all treatments, despite high levels of Cu in their post-weaning diets. The antioxidant status of piglets was not globally altered by treatments (p > 0.05). For microbiota composition, sow supplementation increased (p < 0.01) richness in bacterial species in the piglet colon, either before or shortly after weaning. Short-chain fatty acids in caecal digesta were increased by sow supplementation in LW piglets before weaning at 16 days of age (p < 0.05). In conclusion, oral supplementations to piglets increased postnatal micronutrient statuses during lactation, but this did not generally persist after weaning. Treatments to sows or piglets did not improve the response of piglets to oxidative stress, but supplementation to sows favoured gut microbiota diversity, particularly in LW piglets.