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Generation Mean Analysis of Net Blotch and Scald Diseases on Barley 大麦网斑病和烫伤病世代平均分析
Pub Date : 2020-10-22 DOI: 10.12691/WJAR-8-4-6
Z. Jalata, F. Mekbib, B. Lakew
The information on the nature and magnitude of genes controlling the resistance to net blotch and scald in barley is useful in resistance breeding. Thus, field experiment was conducted at Holetta, Ethiopia in 2015 on barley using six basic generations of (P1, P2, F1, F2, BC1, and BC2) which was derived from ‘HB42’x‘Sabini’ parental cross which were evaluated in RCB design with three replications to investigate the gene actions and interactions gene effects involved in controlling resistance to net blotch and scald diseases in barley. The result of scaling tests and generation mean analysis indicated the predominance of non-additive gene effects including epistasis gene effects than additive gene effects for all parameters and this was also confirmed by variance component analysis. Moreover, the digenic epistatic model failed to explain variation in generation means for all parameters may due to the presence of higher-order interaction and linkages. In general, the results suggest greater influence of non-additive genes including epistasis in the control of both disease parameters studied making early selection ineffective. And ‘HB42’ cultivar contained double resistance to scald and net blotch diseases which needs further study for its exploitation as source of resistance in improving barley yield.
研究控制大麦网斑病和烫病抗性的基因的性质和大小,对大麦的抗性育种具有重要意义。因此,我们于2015年在埃塞俄比亚的Holetta进行了大田试验,利用‘hb42’与‘sabini’亲本杂交获得的6个基本代(P1、P2、F1、F2、BC1和BC2),在RCB设计中进行了3个重复的评价,研究了控制大麦网斑病和烫病抗性的基因作用和相互作用。标度检验和代均值分析结果表明,包括上位基因效应在内的非加性基因效应在各参数上均优于加性基因效应,方差成分分析也证实了这一点。此外,遗传上位模型未能解释所有参数的代均值变化,这可能是由于存在高阶相互作用和联系。总的来说,结果表明,包括上位性在内的非加性基因在控制所研究的两种疾病参数方面的影响更大,这使得早期选择无效。HB42具有对烫伤病和网斑病的双重抗性,作为大麦增产的抗性来源有待进一步研究。
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引用次数: 2
Changing Pattern of Rainfall Amount and Raindays in Samaru, Northern Nigeria and Their Implications on Crop Production 尼日利亚北部萨马鲁地区降雨量和日数变化格局及其对作物生产的影响
Pub Date : 2020-10-14 DOI: 10.12691/wjar-8-4-5
A. Lawal, A. Yamusa
The rainfall amount and number of rainy days determines the length of growing season, which equally determines the types of crops to be planted in a particular region. This study analyzed the pattern of rainfall in Samaru Zaria, Kaduna State, Northern part of Nigeria from 1961-2017 using data collected from the Institute for Agricultural Research Samaru, Zaria. The result from this research reveals that an inverse relationship exists between the amount of rainfall and the rainy days. Hence, the higher the rainfall amount the lower the rainy days and vice versa. This is attributed to the current change in climate which increases the intensity of annual rainfall but shortens the duration of the rainy days. The equations generated for amount of rainfall pattern was (y= 3.162x - 5270.9) and number of rainy days was obtained as (y = -0.172x + 417.85). As expected, the months of November to March had the lowest mean rainy days (MRDs) <1 day. As a recurrent pattern for the period of study, the onset of appreciable rainy days was observed in the month of April and the number of rainy days increased steadily to reach its peak in August and a sharp decline in October. From the obtained data, the onset and cessation of rainfall could be predicted to fall towards the end of April and September respectively.
降雨量和雨天的数量决定了生长季节的长短,同样也决定了在特定地区种植的作物类型。本研究使用从萨马鲁农业研究所收集的数据,分析了1961-2017年尼日利亚北部卡杜纳州萨马鲁扎里亚的降雨模式。研究结果表明,降雨量与降雨日数呈反比关系。因此,降雨量越大,阴雨天数越少,反之亦然。这是由于目前的气候变化,增加了年降雨量的强度,但缩短了雨天的持续时间。生成的降水模式量方程为(y= 3.162x - 5270.9),得到的降雨日数为(y= -0.172x + 417.85)。正如预期的那样,11月至3月的平均阴雨日数(MRDs) <1天最低。作为研究期间的经常性模式,在4月份观察到明显的雨天的开始,雨天的数量稳步增加,在8月达到高峰,10月急剧减少。根据所获得的资料,可以预测降雨的开始和停止分别在4月底和9月底。
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引用次数: 3
Value Chain Analysis of Wheat (Triticum Aestivum) in Dembecha District, Ethiopia 埃塞俄比亚登贝查地区小麦(Triticum Aestivum)价值链分析
Pub Date : 2020-09-26 DOI: 10.12691/WJAR-8-4-3
Yonnas Addis, Worku Mengesha
This study aimed to analyze the value chain of wheat in Dembecha district of West Gojjam zone. A total of 130 farm households, 30 traders, 2 bakers, 1 processor, 4 cooperatives and 10 consumers were used to collect the data. Functional and institutional approach of value chain analysis showed input providers, producers, cooperatives, collectors, wholesalers, retailers, and processors were found to be important wheat value chain actors. Four firms concentration ratio (CR4) was found to be 39.78% and there were information asymmetry and observed barriers to enter into wheat market. Buying, selling and pricing strategies showed deviation of wheat market from competitive market norms. Analysis of marketing margins revealed that processors received the highest (39.72%) marketing margin and retailers received the least marketing margins (4.85%) from consumers’ price. Even if wheat in the study area deviate from competitive market norms as a result of oligopolistic market structure and conduct, profitability analysis of wheat market showed all market actors operated at profitable level. Supporting farmers and increase productivity through practical research, develop pie-growing mechanisms among actors, founding agro-processing firm, providing infrastructural facilities, designing advance way of disseminating market information, determining appropriate pricing strategies should be done to strength value chain development.
本研究旨在分析西Gojjam地区Dembecha地区的小麦价值链。共有130个农户、30个贸易商、2个面包师、1个加工商、4个合作社和10个消费者被用于收集数据。价值链分析的功能和制度方法表明,投入提供者、生产者、合作社、收集者、批发商、零售商和加工商是小麦价值链的重要参与者。四家企业集中度(CR4)为39.78%,存在信息不对称和进入壁垒。小麦市场的购买、销售和定价策略均表现出对竞争性市场规范的偏离。市场利润率分析显示,加工者从消费者价格中获得的市场利润率最高(39.72%),零售商从消费者价格中获得的市场利润率最低(4.85%)。即使研究区域的小麦由于寡头垄断的市场结构和行为而偏离了竞争性市场规范,小麦市场的盈利能力分析表明,所有市场参与者都在盈利水平上运作。应通过实际研究支持农民并提高生产力,在行动者之间建立馅饼种植机制,建立农产品加工企业,提供基础设施,设计传播市场信息的先进方式,确定适当的定价策略,以加强价值链的发展。
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引用次数: 0
Management of Bacterial Wilt Using Grafting Technique in Tomato (Ralstonia solanacearum) 嫁接技术防治番茄青枯病
Pub Date : 2020-09-26 DOI: 10.12691/WJAR-8-4-4
Sudeep Pandey, Utsav Koirala, Prabin Acharya, S. Shrestha
Tomato farmers have been facing a major threat of entire crop loss because of bacterial wilt of tomato. Grafting of highly productive scion onto resistant rootstock is one of the best methods to prevent crops from bacterial wilt disease. An experiment was conducted using tomato (RS-101) and eggplant (KER-DC-117) rootstocks grafted with six scion varieties in different combinations to make total of nine treatments replicated three times in completely randomized design in the naturally infected farmer’s fields at Dahachok, Nepal in 2019. All the three treatments with eggplant rootstock (KER-DC-117) were found to be resistant. Karma 777, Shrijana and Samjhana scions grafted onto tomato rootstock (RS-101) were moderately resistant, whereas Sarita and Karma 555 grafted onto tomato rootstock (RS-101) were moderately susceptible. Non-grafted Sarita was highly susceptible with 100% disease incidence. There were no bacterial browning and bacterial oozing in treatments with tomato rootstock (RS-101). The yield and number of fruits was maximum with Karma 444 grafted onto eggplant rootstock (KER-DC-117). So, Karma 444 + ER (KER-DC-117) is the best scion-rootstock combination to manage bacterial wilt against tomato at Dahachok, Kathmandu.
由于番茄细菌性枯萎病,番茄种植者一直面临着整个作物损失的重大威胁。将高产接穗嫁接到抗病砧木上是预防作物青枯病的最佳方法之一。2019年,在尼泊尔达哈乔克自然感染的农民田间,采用完全随机设计,将番茄(RS-101)和茄子(KER-DC-117)砧木以不同组合嫁接6个接穗品种,共9个处理重复3次。3种砧木(KER-DC-117)处理均具有抗性。嫁接到番茄砧木(RS-101)上的Karma 777、Shrijana和Samjhana接穗具有中等抗性,嫁接到番茄砧木(RS-101)上的Sarita和Karma 555接穗具有中等抗性。未嫁接的莎丽塔易感,发病率100%。番茄砧木(RS-101)处理无细菌褐变和渗菌现象。嫁接到茄子砧木(KER-DC-117)上的Karma 444产量和果实数最高。因此,Karma 444 + ER (KER-DC-117)是防治番茄青枯病的最佳接穗-砧木组合。
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引用次数: 0
Evaluation of the Performance of Sweet Potato (Ipomoea batatas) Clones under Water Stress in the Coastal Lowlands of Kenya 肯尼亚沿海低地甘薯无性系在水分胁迫下的生产性能评价
Pub Date : 2020-09-17 DOI: 10.12691/WJAR-8-4-2
B. Abdallah, S. Githiri, W. Kariuki, Hemedi Mkuzi Saha
The production of sweet potato (Ipomoea batatas (L.) Lam.) in coastal Kenya is diminishing due to unreliable rainfall, coupled with farmers’ use of unimproved sweet potato varieties that are not drought tolerant. A study was conducted at Pwani University from 2016 to 2017, to identify sweet potato clones with best performance under low water stress. Nine clones (6.1A, 4.10, 7.8, 15.10, 7.6AO, 10.10B, 4.2B, 7.6B and 4.2A) and a farmer-preferred variety “Rabai” were evaluated under the following four water stress levels: (i) Watering for the first two months after planting and stressing the plants for the next three months - S3, (ii) Watering for the first three months after planting and stressing the plants for the next two months - S2, (iii) Watering for the first four months after planting and stressing the plants for the next one month - S1, and (iv) Watering throughout the growing period and not stressing the plants at all - S0. A randomized complete block design was used, with factorial arrangement of treatments. Treatments were replicated three times. Tuber circumference was reduced by low water stress irrespective of the time it started, relative to the growing season. Water stress that was imposed early in the season, at three or two months after planting, reduced tuber yield by about 52 and 70%, respectively. Sweet potato clones C2 and C8 performed relatively well across seasons, irrespective of the water stress level. Farmers are likely to realize improved tuber yields by planting sweet potato early in the rainfall season to ensure adequate soil water during the first four months of crop growth. Sweet potato clones C2 and C8 are therefore recommended for multi-locational trials in coastal lowland Kenya, to ascertain their performance across agro-ecological zones.
甘薯(Ipomoea batatas)的生产由于降雨不可靠,加上农民使用不耐干旱的未经改良的甘薯品种,肯尼亚沿海地区的甘薯数量正在减少。2016年至2017年,Pwani大学进行了一项研究,以确定在低水分胁迫下表现最佳的甘薯无性系。对9个无性系(6.1A、4.10、7.8、15.10、7.6AO、10.10B、4.2B、7.6B和4.2A)和一个农民首选品种“拉拜”在以下4个水分胁迫水平下进行了评价:(i)种植后的头两个月浇水,并在接下来的三个月给植物施加压力- S3, (ii)种植后的头三个月浇水,并在接下来的两个月给植物施加压力- S2, (iii)种植后的头四个月浇水,并在接下来的一个月给植物施加压力- S1,以及(iv)在整个生长期间浇水,根本不给植物施加压力- S0。采用随机完全区组设计,对治疗进行阶乘安排。实验重复了三次。相对于生长季节而言,低水分胁迫使块茎周长减小,与开始时间无关。在种植后3个月或2个月的季节早期施加水分胁迫,块茎产量分别减少约52%和70%。甘薯无性系C2和C8在不同季节表现相对较好,与水分胁迫水平无关。农民可以通过在降雨季节早期种植甘薯来提高块茎产量,以确保作物生长的头四个月有足够的土壤水分。因此,建议在肯尼亚沿海低地进行多地点试验红薯C2和C8无性系,以确定它们在整个农业生态区的表现。
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引用次数: 3
Field Evaluation of Some Varieties/Accessions of Maize for Their Performances in a Derived Savannah Belt of Nigeria 尼日利亚萨凡纳衍生带部分玉米品种/材料田间性能评价
Pub Date : 2020-09-09 DOI: 10.12691/WJAR-8-4-1
Abba Nathan Yatai, Chukwulobe Modesta Ngozi, Echezona Bonaventure Chukwujindu
Vegetation growth is functionally dependent on climate. Though increased yields have been attained through plant breeding, yet it is well established that genetic factors alone cannot cause a plant character to develop to its maximum potential without a favorable environment. Plants can be made to adapt to conditions which deviate from their natural habitat through breeding and acclimatization. Three local, Oba super-2 hybrid and seventeen maize accessions/varieties newly developed by the Institute for Agricultural Research (IAR) Samaru Zaria, in collaboration with the International Institute of Tropical Agriculture (IITA), for the northern guinea savanna belt, were evaluated for their relative yield performances in the derived savanna belt of Nigeria. The assessments were carried out at the Department of Crop Science teaching and research farm, University of Nigeria, Nsukka. Parameters assessed include field germination percentage, days to first tassel appearance, days to fifty percent tassel appearance, days to fifty percent silking, percentage, double cob per stand, percentage triple cob per stand, plant height, days to maturity, percentage harvest index, cob weight, grain weight per ear e.tc. Correlation and path analysis were used to ascertain the degree of association of traits with yield. Biomass weight was observed to have the highest direct positive influence on yield. Therefore, varieties with high biomass weight (Sammaz-14, 17 and 18) consistently produced high yield. This results could be useful to breeders as they work to produce maize varieties that will perform better in the derived savanna zone of the country. That will make for increased availability and choice of planting materials for farmers and ultimately, increased productivity and sustainability of maize production in the derived Savanna Zones of Nigeria. Further screening of these accessions should also be encouraged in the zone until adaptation is established.
植被生长在功能上依赖于气候。虽然通过植物育种提高了产量,但众所周知,如果没有良好的环境,单靠遗传因素是不能使植物性状发展到最大潜力的。植物可以通过繁殖和驯化来适应偏离其自然栖息地的条件。农业研究所(IAR) Samaru Zaria与国际热带农业研究所(IITA)合作为几内亚北部稀树草原带新开发的3个地方杂交品种Oba super-2和17个玉米材料/品种在尼日利亚衍生稀树草原带的相对产量表现进行了评价。这些评估是在尼日利亚大学作物科学系的教学和研究农场进行的。评估的参数包括田间发芽率、出穗天数、出穗天数至50%、出丝天数至50%、百分比、每株双穗轴、每株三穗轴百分比、株高、成熟天数、收获指数百分比、穗轴重量、每穗粒重等。利用相关分析和通径分析确定各性状与产量的相关程度。生物量对产量的直接正向影响最大。因此,高生物量的品种(Sammaz-14、17和18)持续高产。这一结果可能对育种者有用,因为他们正在努力生产在该国衍生热带稀树草原地区表现更好的玉米品种。这将增加农民种植材料的可获得性和可选择性,并最终提高尼日利亚热带稀树草原衍生区玉米生产的生产力和可持续性。还应鼓励在保护区对这些物种进行进一步筛选,直到适应情况确定为止。
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引用次数: 1
Machine Crop Parameters’ Model of Spike - Tooth Thresher for Soybean 大豆穗齿脱粒机机械作物参数模型
Pub Date : 2020-07-22 DOI: 10.12691/WJAR-8-3-5
T. A. Ilori, T. Dauda, Adewumi I.O
The goal of regular modification of threshing machine is to increase soybean processing efficiency. This study was carried out to deconstruct the thresher’s parameter - output relationship and to evolve a model for such relationship at the Engineering workshop of the Federal College of Agriculture, Ibadan, Oyo State Nigeria. Unthreshed soybeans, CGX14x1448 were used to evaluate the performance of a modified spike tooth thresher fabricated at the Workshop. The summary statistics of the machine output showed that concave clearance had percentage unthreshed soybean of 16.98%, percentage mechanically damaged seed of 8.625%, blown seed of 23.403kg and high seed loss of 44.241kg. Both the mean cleaning efficiency (94.175) and threshing efficiency (83.022) were very high. The trend of the cylinder speed however contrasts with those of concave clearance and moisture content. The percentage unthreshed (83.212%), percentage mechanical damage (16.792%) were in contrast to each other. Similarly, the threshing (44.108) and cleaning (23.321) efficiency were low. The relationships between cylinder speed and machine output showed an increasing trends for blown seed, percentage damaged seed and seed loss. Concave clearance - threshing efficiency can be predicted using 3 most parsimonious models (xy-inverse, exponential and simple linear model). High adjusted coefficients of determinations (R2) were obtained for the best model of cylinder speed - threshing efficiency model. These were 0.9997 for the xy inverse and 0.9998 for quadratic 0.993 for exponential model with estimation variance of 0.00047 (quadratic), 0.000 for inverse xy and 0.000191 (exponential model). The practical application of the models is in the specificity of the measurement of the relationship between thresher’s parameters and output.
对脱粒机进行定期改造的目的是提高大豆的加工效率。本研究是在尼日利亚奥约州伊巴丹联邦农业学院的工程研讨会上进行的,目的是解构脱粒机的参数-输出关系,并为这种关系建立一个模型。以未脱粒大豆CGX14x1448为试验材料,对车间生产的脱粒机进行了性能评价。对机器产量的汇总统计表明,凹间隙未脱粒大豆占16.98%,机械损坏种子占8.625%,吹散种子占23.403kg,高损失种子占44.241kg。平均清洗效率(94.175)和脱粒效率(83.022)均较高。然而,滚筒速度的变化趋势与凹间隙和含水率的变化趋势相反。未脱粒率为83.212%,机械损伤率为16.792%。脱粒效率(44.108)和清洗效率(23.321)均较低。汽缸转速与机器产量的关系表现出吹种率、破损率和籽粒损失率呈增加趋势。凹间隙脱粒效率可以用3种最简洁的模型(x -逆模型、指数模型和简单线性模型)进行预测。圆筒速度-脱粒效率模型的最佳模型具有较高的校正系数(R2)。这些是0.9997的xy逆和0.9998的二次0.993指数模型的估计方差为0.00047(二次),0.000的逆xy和0.000191(指数模型)。该模型的实际应用在于对脱粒机参数与产量关系的测量的专用性。
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引用次数: 0
Genetic Associations under Aphids and Rosette Stress in Groundnut (Arachis hypogaea L.) 花生蚜虫和莲座胁迫下的遗传关联
Pub Date : 2020-07-20 DOI: 10.12691/WJAR-8-3-4
V. Musa, S. E. Akogu, F. Agbaji, H. Adah
Groundnut production is largely constrained by biotic stresses with groundnut rosette virus disease seriously contributing to losses in yield in Nigeria and sub Saharan Africa. This study was conducted to understand performances and correlations between aphid resistance, rosette resistance and other quantitative characters. Two aphid resistance, one rosette resistance, one aphid susceptible and one rosette susceptible lines were used as parents to develop F1s, F2s, BC1P1 and BC1P2. The seventeen generations obtained were evaluated along with three checks in three replications using randomized complete block design. The F1 generations had higher mean performance compared to the parents suggesting heterosis. The segregating generations (F2s, BC1P1 and BC1P2) had mean values higher or lower than the parents due to transgressive genes. The genotypic correlation coefficients in contrasting magnitudes and directions exceeded those of the corresponding phenotypic correlation coefficients for most of the character pairs indicating that the correlations were more genetic than environmental in the three sets of crosses studied.
花生生产在很大程度上受到生物胁迫的制约,花生花环病毒病严重造成尼日利亚和撒哈拉以南非洲的产量损失。本研究旨在了解抗蚜、抗结花等数量性状的表现及其相互关系。以2个抗蚜系、1个抗莲座系、1个感蚜系和1个感蚜系为亲本,培养F1s、F2s、BC1P1和BC1P2。采用随机完全区组设计,对获得的17代进行评估,并在3个重复中进行3次检查。F1代的平均表现高于亲本,表明杂种优势。各分离代(F2s、BC1P1和BC1P2)的平均值均因基因入侵而高于或低于亲本。绝大多数性状对的基因型相关系数在对比大小和方向上均大于相应的表型相关系数,说明遗传相关性大于环境相关性。
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引用次数: 0
Compositional Analysis of Volatile Compounds of Paspangiri - A Mixture of Leaves of Five Rutaceae Plants Used as an Effective Remedy for Respiratory System Microbial Infections in Traditional Systems of Medicine 传统医学中治疗呼吸道微生物感染的有效药物五种芸香科植物叶片的混合物——凡香的挥发性成分分析
Pub Date : 2020-07-03 DOI: 10.12691/WJAR-8-3-3
R. Dharmadasa, Jayasinghe J.A.T.U., Arawwawala L.D.A.M., Fonseka D.L.C.K
Steaming with aromatic poly herbal formulations has been practiced as an effective treatment for curing or controlling infectious diseases, deterring of pest species, destroying of unwanted microbial contaminations by several cultures. Steaming of Pasppangiri, a mixture of leaves of five species (Citrus aurantium Linn, Citrus aurantifolia (Christm. & Panzer) Swingle, Citrus sinensis Linn, Atlantia ceylanica (Am.) Oliver, Citrus reticulata Blanco) has been used for an effective treatment for curing of microbial infected, respiratory system related diseases. However, information on volatile compounds, compositional analysis of Paspangiri is lacking or scattered. Leaves of five citrus species were collected from previously authenticated plants grown in same soil and climatic conditions. Essential oil was separated by hydro distillation method. The chemical composition of the essential oil of Paspangiri was analysed using gas chromatography-mass spectrometry (GC-MS). Results revealed that the number of compounds observed in each species were varied as Citrus sinensis (27)>aurantium (25) >Citrus reticulata (20)>Citrus aurantifolia (19)>Atlantia ceylanica (5) respectively. The major bioactive molecules identified in essential oils of Paspangiri such as linalool, limonene, caryophyllene, eugenol, z-citral, ∞- pinene, sabinene, and myrcene, either singularly or as a mixture have been exhibited antimicrobial properties. Therefore, the present study, partially validate the traditional claims of inhaling of Paspangiri steam for prevention or curing of infectious disease.
用芳香的多草药配方蒸制已被实践为一种有效的治疗方法,用于治疗或控制传染病,阻止害虫物种,通过几种培养破坏有害的微生物污染。蒸的Pasppangiri,五种叶子的混合物(Citrus aurantium Linn, Citrus aurantifolia, Christm。& Panzer) swing, Citrus sinensis Linn, atlantic ceylanica (Am.)Oliver, Citrus reticulata Blanco)已被用于治疗微生物感染,呼吸系统相关疾病的有效治疗。然而,对其挥发性化合物、成分分析等方面的资料缺乏或分散。在相同的土壤和气候条件下,从以前鉴定的植物中收集了五种柑橘的叶子。采用水力蒸馏法分离精油。采用气相色谱-质谱联用(GC-MS)技术分析了白菖蒲挥发油的化学成分。结果表明,各种中化合物的含量差异较大,分别为柑橘(Citrus sinensis)(27)、柑橘(Citrus aurantium)(25)、柑橘(Citrus reticulata)(20)、柑橘(Citrus aurantifolia)(19)、柑橘(Atlantia ceylanica)(5)。在香椿精油中发现的主要生物活性分子,如芳樟醇、柠檬烯、石竹烯、丁香酚、z-柠檬醛、∞-蒎烯、沙滨烯和月桂烯,无论是单独的还是混合的,都表现出抗菌性能。因此,本研究部分验证了吸入帕潘吉里蒸汽预防或治疗传染病的传统说法。
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引用次数: 0
Identification, Determination and Quantification of Indole-3-Acetic Acid Produced by Pseudomonas aeruginosa UPMP3 and Its Effect on The Growth of Oil Palm (Elaeis guineensis Jacq) 铜绿假单胞菌UPMP3产吲哚-3-乙酸的鉴定、测定与定量及其对油棕生长的影响
Pub Date : 2020-06-20 DOI: 10.12691/WJAR-8-3-2
W. Parvin, M. Rahman, Nisha Govender, M. Wong
Pseudomonas species have founded as greatest and potentially most promising group of plant growth promoting rhizobacteria (PGPR). Pseudomonas aeruginosa UPMP3 is an important PGPR isolated from oil palm rhizosphere. This rhizobacteria is likely to synthesize and release phytohormone indole-3 acetic acid (IAA). Production of IAA is one of the main reasons to promote plant growth and yield. The aim of this study was to detect, identify and quantify the IAA production by P. aeruginosa UPMP3 in vitro and its influence on oil palm seedling growth. Nutrient broth medium supplemented with 1-5 mg/ml L-tryptophan and without L- tryptophan were used for bacterial culture. The pH levels of culture media were optimized under shaken and static conditions and incubated at 28±2°C in different incubation periods. The production of IAA by P. aeruginosa UPMP3 was extracted, purified, detected and quantified by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) analyses. Production of IAA was quantified by HPLC in liquid culture and achieved 12.08µg/ml with a retention time of 13.711 min. On the other hand, the maximum 52 µg/ml IAA was recorded in the medium supplemented with 4 mg/ml L- tryptophan in compare to control. The optimum pH level of the culture medium was recorded as 7 under shaken conditions at 150 rpm with 5 days incubation. The influence of IAA produced by the UPMP3 on oil palm seedling growth was carried out in the pot experiment. The germinated oil palm seedlings were treated with the extract of bacterial strain and observed a positive effect on seedling growth in respect to average root and leaf number, root, shoot, and leaf length compare to the synthetic IAA and the control.
假单胞菌是促进植物生长的根瘤菌(PGPR)中最大和最有潜力的一类。铜绿假单胞菌UPMP3是从油棕根际分离到的一种重要的PGPR。该菌可能合成并释放植物激素吲哚-3乙酸(IAA)。IAA的产生是促进植物生长和产量的主要原因之一。本研究旨在检测、鉴定和定量铜绿假单胞菌UPMP3体外产生IAA及其对油棕幼苗生长的影响。采用添加1-5 mg/ml L-色氨酸和不添加L-色氨酸的营养肉汤培养基进行细菌培养。在摇摇和静态条件下优化培养基的pH值,并在28±2℃条件下进行不同培养时间的培养。采用薄层色谱(TLC)和高效液相色谱(HPLC)对P. aeruginosa UPMP3产IAA进行提取、纯化、检测和定量。液相高效液相色谱法测定IAA在液体培养中的产量为12.08µg/ml,保留时间为13.711 min,而在添加4 mg/ml色氨酸的培养基中,IAA的最高产量为52µg/ml。在150转/分振荡条件下,培养5天,培养基的最佳pH值为7。通过盆栽试验,研究了UPMP3产生的IAA对油棕幼苗生长的影响。与合成IAA和对照相比,该菌株提取物对发芽油棕幼苗的平均根叶数、根、芽和叶长均有显著的促进作用。
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引用次数: 1
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World Journal of Agricultural Research
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