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Current Understandings and Open Hypotheses on Extracellular Circular RNAs. 关于细胞外环状 RNA 的现有认识和开放性假设。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-01 DOI: 10.1002/wrna.1872
Jasper Verwilt, Marieke Vromman

Circular RNAs (circRNAs) are closed RNA loops present in humans and other organisms. Various circRNAs have an essential role in diseases, including cancer. Cells can release circRNAs into the extracellular space of adjacent biofluids and can be present in extracellular vesicles. Due to their circular nature, extracellular circRNAs (excircRNAs) are more stable than their linear counterparts and are abundant in many biofluids, such as blood plasma and urine. circRNAs' link with disease suggests their extracellular counterparts have high biomarker potential. However, circRNAs and the extracellular space are challenging research domains, as they consist of complex biological systems plagued with nomenclature issues and a wide variety of protocols with different advantages and disadvantages. Here, we summarize what is known about excircRNAs, the current challenges in the field, and what is needed to improve extracellular circRNA research.

环状 RNA(circRNA)是存在于人类和其他生物体内的闭合 RNA 环。各种 circRNA 在疾病(包括癌症)中发挥着重要作用。细胞可将 circRNA 释放到邻近生物流体的细胞外空间,也可存在于细胞外囊泡中。由于细胞外 circRNAs(excircRNAs)具有环状性质,因此比其线性对应物更稳定,在血浆和尿液等许多生物流体中含量丰富。circRNAs 与疾病的联系表明,其细胞外对应物具有很高的生物标记潜力。然而,circRNAs 和细胞外空间是极具挑战性的研究领域,因为它们由复杂的生物系统组成,存在着命名问题和优缺点各异的各种方案。在此,我们总结了人们对细胞外 circRNA 的了解、该领域目前面临的挑战以及改进细胞外 circRNA 研究的需要。
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引用次数: 0
Decoding the role of RNA sequences and their interactions in influenza A virus infection and adaptation. 解码 RNA 序列及其相互作用在甲型流感病毒感染和适应中的作用。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-01 DOI: 10.1002/wrna.1871
Satya P Sharma, Mamta Chawla-Sarkar, Rajat Sandhir, Dipanjan Dutta

Influenza viruses (types A, B, C, and D) belong to the family orthomyxoviridae. Out of all the influenza types, influenza A virus (IAV) causes human pandemic outbreaks. Its pandemic potential is predominantly attributed to the genetic reassortment favored by a broad spectrum of host species that could lead to an antigenic shift along with a high rate of mutations in its genome, presenting a possibility of subtypes with heightened pathogenesis and virulence in humans (antigenic drift). In addition to antigenic shift and drift, there are several other inherent properties of its viral RNA species (vRNA, vmRNA, and cRNA) that significantly contribute to the success of specific stages of viral infection. In this review, we compile the key features of IAV RNA, such as sequence motifs and secondary structures, their functional significance in the infection cycle, and their overall impact on the virus's adaptive and evolutionary fitness. Because many of these motifs and folds are conserved, we also assess the existing antiviral approaches focused on targeting IAV RNA. This article is categorized under: RNA Structure and Dynamics > Influence of RNA Structure in Biological Systems RNA Interactions with Proteins and Other Molecules > Protein-RNA Interactions: Functional Implications RNA in Disease and Development > RNA in Disease.

流感病毒(甲型、乙型、丙型和丁型)属于正粘病毒科。在所有流感病毒类型中,甲型流感病毒(IAV)会导致人类流感大爆发。甲型流感病毒之所以具有大流行的潜能,主要是由于其基因重组受到广泛宿主物种的青睐,这可能导致抗原转变以及基因组的高突变率,从而可能产生对人类具有更强致病性和毒力的亚型(抗原漂移)。除抗原转移和漂移外,病毒 RNA(vRNA、vmRNA 和 cRNA)还具有其他一些固有特性,这些特性对病毒感染特定阶段的成功起着重要作用。在这篇综述中,我们梳理了 IAV RNA 的关键特征,如序列基序和二级结构、它们在感染周期中的功能意义,以及它们对病毒适应性和进化适应性的总体影响。由于这些基序和褶皱中有许多是保守的,因此我们还评估了现有的以 IAV RNA 为靶标的抗病毒方法。本文归类于RNA 结构与动力学 > RNA 结构对生物系统的影响 RNA 与蛋白质和其他分子的相互作用 > 蛋白质与 RNA 的相互作用:疾病和发育中的 RNA > 疾病中的 RNA。
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引用次数: 0
The Definition of RNA Age Related to RNA Sequence Changes. 与RNA序列变化相关的RNA年龄的定义。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-11-01 DOI: 10.1002/wrna.1876
Zhongneng Xu, Shuichi Asakawa

Ribonucleic acid (RNA) undergoes dynamic changes in its structure and function under various intracellular and extracellular conditions over time. However, there is a lack of research on the concept of the RNA age to describe its diverse fates. This study proposes a definition of RNA age to address this issue. RNA age was defined as a sequence of numbers wherein the elements in the sequence were the nucleotide ages of the ribonucleotide residues in the RNA. Mean nucleotide age was used to represent RNA age. This definition describes the temporal properties of RNAs that have undergone diverse life histories and reflects the dynamic state of each ribonucleotide residue, which can be expressed mathematically. Notably, events (including base insertions, base deletions, and base substitutions) are likely to cause RNA to become younger or older when using mean nucleotide ages to represent the RNA age. Although information, including the presence of added markers in RNA, chemical modification structure of the RNA, and the excision of introns in the mRNA in cells, may provide a basis for identifying RNA age, little is known about determining the RNA age of extracellular RNA in the wild. Nonetheless, we believe that RNA age has an important relationship with the diverse biological properties of RNA under intracellular and extracellular conditions. Therefore, our proposed definition of RNA age offers new perspectives for studying dynamic changes in RNA function, RNA aging, ancient RNA, environmental RNA, and the ages of other biomolecules.

随着时间的推移,核糖核酸(RNA)的结构和功能在不同的细胞内和细胞外条件下发生动态变化。然而,缺乏对RNA时代概念的研究来描述其多样化的命运。本研究提出了RNA年龄的定义来解决这个问题。RNA年龄被定义为一个数字序列,其中序列中的元素是RNA中核糖核苷酸残基的核苷酸年龄。平均核苷酸年龄表示RNA年龄。这个定义描述了经历了不同生活史的rna的时间性质,反映了每个核糖核苷酸残基的动态状态,可以用数学的方式表达。值得注意的是,当使用平均核苷酸年龄来表示RNA年龄时,事件(包括碱基插入、碱基缺失和碱基替换)可能会导致RNA变得更年轻或更老。虽然信息,包括RNA中添加标记物的存在、RNA的化学修饰结构以及细胞中mRNA内含子的切除,可能为鉴定RNA年龄提供了基础,但对于确定野生细胞外RNA的RNA年龄知之甚少。尽管如此,我们认为RNA年龄与RNA在细胞内和细胞外条件下的多种生物学特性有着重要的关系。因此,我们提出的RNA年龄定义为研究RNA功能、RNA老化、古代RNA、环境RNA和其他生物分子年龄的动态变化提供了新的视角。
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引用次数: 0
The landscape of lncRNAs in cell granules: Insights into their significance in cancer. 细胞颗粒中的 lncRNAs 景观:洞察它们在癌症中的意义
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-09-01 DOI: 10.1002/wrna.1870
Carolina Mathias, Ana Carolina Rodrigues, Suelen Cristina Soares Baal, Alexandre Luiz Korte de Azevedo, Vanessa Nascimento Kozak, Leticia Ferreira Alves, Jaqueline Carvalho de Oliveira, Sonia Guil, Daniela Fiori Gradia

Cellular compartmentalization, achieved through membrane-based compartments, is a fundamental aspect of cell biology that contributes to the evolutionary success of cells. While organelles have traditionally been the focus of research, membrane-less organelles (MLOs) are emerging as critical players, exhibiting distinct morphological features and unique molecular compositions. Recent research highlights the pivotal role of long noncoding RNAs (lncRNAs) in MLOs and their involvement in various cellular processes across different organisms. In the context of cancer, dysregulation of MLO formation, influenced by altered lncRNA expression, impacts chromatin organization, oncogenic transcription, signaling pathways, and telomere lengthening. This review synthesizes the current understanding of lncRNA composition within MLOs, delineating their functions and exploring how their dysregulation contributes to human cancers. Environmental challenges in tumorigenesis, such as nutrient deprivation and hypoxia, induce stress granules, promoting cancer cell survival and progression. Advancements in biochemical techniques, particularly single RNA imaging methods, offer valuable tools for studying RNA functions within live cells. However, detecting low-abundance lncRNAs remains challenging due to their limited expression levels. The correlation between lncRNA expression and pathological conditions, particularly cancer, should be explored, emphasizing the importance of single-cell studies for precise biomarker identification and the development of personalized therapeutic strategies. This article is categorized under: RNA Export and Localization > RNA Localization RNA in Disease and Development > RNA in Disease RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes.

通过以膜为基础的区室实现的细胞区隔是细胞生物学的一个基本方面,有助于细胞进化的成功。虽然细胞器历来是研究的重点,但无膜细胞器(MLOs)正逐渐成为关键角色,表现出不同的形态特征和独特的分子组成。最近的研究强调了长非编码 RNA(lncRNA)在 MLOs 中的关键作用,以及它们在不同生物体的各种细胞过程中的参与。就癌症而言,受 lncRNA 表达改变的影响,MLO 形成失调会影响染色质组织、致癌转录、信号通路和端粒延长。这篇综述综述了目前对MLO内lncRNA组成的理解,描述了它们的功能,并探讨了它们的失调是如何导致人类癌症的。肿瘤发生过程中的环境挑战,如营养匮乏和缺氧,会诱导应激颗粒,促进癌细胞的存活和发展。生化技术的进步,尤其是单 RNA 成像方法,为研究活细胞内的 RNA 功能提供了宝贵的工具。然而,由于低丰度 lncRNA 的表达水平有限,检测它们仍然具有挑战性。应探讨lncRNA表达与病理状况(尤其是癌症)之间的相关性,强调单细胞研究对于精确鉴定生物标志物和开发个性化治疗策略的重要性。本文归类于RNA 输出和定位 > RNA 定位 RNA 在疾病和发育中的作用 > RNA 在疾病中的作用 RNA 与蛋白质和其他分子的相互作用 > RNA 蛋白复合物。
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引用次数: 0
Orchestrated centers for the production of proteins or "translation factories". 精心策划的蛋白质生产中心或 "翻译工厂"。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-07-01 DOI: 10.1002/wrna.1867
Robert A Crawford, Matthew Eastham, Martin R Pool, Mark P Ashe

The mechanics of how proteins are generated from mRNA is increasingly well understood. However, much less is known about how protein production is coordinated and orchestrated within the crowded intracellular environment, especially in eukaryotic cells. Recent studies suggest that localized sites exist for the coordinated production of specific proteins. These sites have been termed "translation factories" and roles in protein complex formation, protein localization, inheritance, and translation regulation have been postulated. In this article, we review the evidence supporting the translation of mRNA at these sites, the details of their mechanism of formation, and their likely functional significance. Finally, we consider the key uncertainties regarding these elusive structures in cells. This article is categorized under: Translation Translation > Mechanisms RNA Export and Localization > RNA Localization Translation > Regulation.

人们对蛋白质如何从 mRNA 生成的机理有了越来越深入的了解。然而,人们对蛋白质是如何在拥挤的细胞内环境中协调生成的却知之甚少,尤其是在真核细胞中。最近的研究表明,存在着协调生产特定蛋白质的局部位点。这些位点被称为 "翻译工厂",并被推测在蛋白质复合物形成、蛋白质定位、遗传和翻译调控中发挥作用。在本文中,我们回顾了支持在这些位点翻译 mRNA 的证据、其形成机制的细节及其可能的功能意义。最后,我们将探讨细胞中这些难以捉摸的结构的主要不确定性。本文归类于翻译 翻译 > 机制 RNA 输出和定位 > RNA 定位 翻译 > 调节。
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引用次数: 0
Pericentromeric satellite RNAs as flexible protein partners in the regulation of nuclear structure. 核周边卫星 RNA 是调节核结构的灵活蛋白质伙伴。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-07-01 DOI: 10.1002/wrna.1868
Mariana Lopes, Sandra Louzada, Margarida Gama-Carvalho, Raquel Chaves

Pericentromeric heterochromatin is mainly composed of satellite DNA sequences. Although being historically associated with transcriptional repression, some pericentromeric satellite DNA sequences are transcribed. The transcription events of pericentromeric satellite sequences occur in highly flexible biological contexts. Hence, the apparent randomness of pericentromeric satellite transcription incites the discussion about the attribution of biological functions. However, pericentromeric satellite RNAs have clear roles in the organization of nuclear structure. Silencing pericentromeric heterochromatin depends on pericentromeric satellite RNAs, that, in a feedback mechanism, contribute to the repression of pericentromeric heterochromatin. Moreover, pericentromeric satellite RNAs can also act as scaffolding molecules in condensate subnuclear structures (e.g., nuclear stress bodies). Since the formation/dissociation of nuclear condensates provides cell adaptability, pericentromeric satellite RNAs can be an epigenetic platform for regulating (sub)nuclear structure. We review current knowledge about pericentromeric satellite RNAs that, irrespective of the meaning of biological function, should be functionally addressed in regular and disease settings. This article is categorized under: RNA Methods > RNA Analyses in Cells RNA in Disease and Development > RNA in Disease.

近染色质异染色质主要由卫星 DNA 序列组成。尽管卫星 DNA 序列在历史上与转录抑制有关,但有些中心染色质周围的卫星 DNA 序列也会被转录。中心周卫星序列的转录事件发生在高度灵活的生物环境中。因此,中心染色体周围卫星转录的明显随机性引发了有关生物功能归属的讨论。然而,近中心染色质卫星 RNA 在核结构的组织中具有明确的作用。沉默周染色质异染色质依赖于周染色质卫星 RNA,后者在反馈机制中有助于抑制周染色质异染色质。此外,围中心染色质卫星 RNA 还可以在凝聚的亚核结构(如核应激体)中充当支架分子。由于核凝聚体的形成/解离提供了细胞的适应性,因此包心染色质卫星 RNA 可以成为调节(亚)核结构的表观遗传平台。我们回顾了目前有关核周卫星 RNA 的知识,无论其生物学功能的意义如何,都应在常规和疾病环境中加以功能性处理。本文归类于RNA 方法 > 细胞中的 RNA 分析 疾病和发育中的 RNA > 疾病中的 RNA。
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引用次数: 0
Synthetic antibodies for accelerated RNA crystallography. 用于加速 RNA 晶体学的合成抗体。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-07-01 DOI: 10.1002/wrna.1869
Saurja DasGupta

RNA structure is crucial to a wide range of cellular processes. The intimate relationship between macromolecular structure and function necessitates the determination of high-resolution structures of functional RNA molecules. X-ray crystallography is the predominant technique used for macromolecular structure determination; however, solving RNA structures has been more challenging than their protein counterparts, as reflected in their poor representation in the Protein Data Bank (<1%). Antibody-assisted RNA crystallography is a relatively new technique that promises to accelerate RNA structure determination by employing synthetic antibodies (Fabs) as crystallization chaperones that are specifically raised against target RNAs. Antibody chaperones facilitate the formation of ordered crystal lattices by minimizing RNA flexibility and replacing unfavorable RNA-RNA contacts with contacts between chaperone molecules. Atomic coordinates of these antibody fragments can also be used as search models to obtain phase information during structure determination. Antibody-assisted RNA crystallography has enabled the structure determination of 15 unique RNA targets, including 11 in the last 6 years. In this review, I cover the historical development of antibody fragments as crystallization chaperones and their application to diverse RNA targets. I discuss how the first structures of antibody-RNA complexes informed the design of second-generation antibodies and led to the development of portable crystallization modules that have greatly reduced the uncertainties associated with RNA crystallography. Finally, I outline unexplored avenues that can increase the impact of this technology in structural biology research and discuss potential applications of antibodies as affinity reagents for interrogating RNA biology outside of their use in crystallography. This article is categorized under: RNA Structure and Dynamics > RNA Structure, Dynamics and Chemistry RNA Interactions with Proteins and Other Molecules > Protein-RNA Recognition RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes.

RNA 结构对多种细胞过程至关重要。由于大分子结构与功能之间的密切关系,有必要确定功能 RNA 分子的高分辨率结构。X 射线晶体学是用于确定大分子结构的主要技术;然而,解决 RNA 结构问题比解决蛋白质结构问题更具挑战性,这反映在它们在蛋白质数据库(RNA Structure, Dynamics and Chemistry RNA Interactions with Proteins and Other Molecules > Protein-RNA Recognition RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes)中的代表性较差。
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引用次数: 0
Novel insights on the positive correlation between sense and antisense pairs on gene expression. 有义和反义配对基因表达正相关的新见解。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-07-01 DOI: 10.1002/wrna.1864
Subhadeep Das, Maria Paula Zea Rojas, Elizabeth J Tran

A considerable proportion of the eukaryotic genome undergoes transcription, leading to the generation of noncoding RNA molecules that lack protein-coding information and are not subjected to translation. These noncoding RNAs (ncRNAs) are well recognized to have essential roles in several biological processes. Long noncoding RNAs (lncRNAs) represent the most extensive category of ncRNAs found in the human genome. Much research has focused on investigating the roles of cis-acting lncRNAs in the regulation of specific target gene expression. In the majority of instances, the regulation of sense gene expression by its corresponding antisense pair occurs in a negative (discordant) manner, resulting in the suppression of the target genes. The notion that a negative correlation exists between sense and antisense pairings is, however, not universally valid. In fact, several recent studies have reported a positive relationship between corresponding cis antisense pairs within plants, budding yeast, and mammalian cancer cells. The positive (concordant) correlation between anti-sense and sense transcripts leads to an increase in the level of the sense transcript within the same genomic loci. In addition, mechanisms such as altering chromatin structure, the formation of R loops, and the recruitment of transcription factors can either enhance transcription or stabilize sense transcripts through their antisense pairs. The primary objective of this work is to provide a comprehensive understanding of both aspects of antisense regulation, specifically focusing on the positive correlation between sense and antisense transcripts in the context of eukaryotic gene expression, including its implications towards cancer progression. This article is categorized under: RNA Processing > 3' End Processing Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs.

真核生物基因组中有相当一部分要进行转录,从而产生缺乏蛋白质编码信息且不需要翻译的非编码 RNA 分子。这些非编码 RNA(ncRNA)被公认在多个生物过程中发挥着重要作用。长非编码 RNA(lncRNA)是人类基因组中发现的最广泛的一类 ncRNA。许多研究都集中在研究顺式作用的 lncRNA 在调控特定靶基因表达中的作用。在大多数情况下,相应的反义配对对有义基因表达的调控是以负相关(不和谐)的方式进行的,从而导致靶基因受到抑制。然而,有义和反义配对之间存在负相关关系的观点并非普遍有效。事实上,最近有几项研究报告称,植物、萌发酵母和哺乳动物癌细胞中相应的顺反义配对之间存在正相关关系。反义转录本与有义转录本之间的正相关(一致)关系会导致同一基因组位点内有义转录本水平的提高。此外,染色质结构的改变、R 环的形成以及转录因子的招募等机制都会通过反义对加强转录或稳定有义转录本。这项研究的主要目的是全面了解反义调控的两个方面,特别是在真核生物基因表达中有义和反义转录本之间的正相关性,包括其对癌症进展的影响。本文归类于RNA 处理 > 3' 端处理 调控 RNAs/RNAi/Riboswitches > 调控 RNAs。
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引用次数: 0
Mechanisms and regulation of spliceosome-mediated pre-mRNA splicing in Saccharomyces cerevisiae. 酿酒酵母中剪接体介导的前 mRNA 剪接的机制和调控。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-07-01 DOI: 10.1002/wrna.1866
Katherine Anne Senn, Aaron A Hoskins

Pre-mRNA splicing, the removal of introns and ligation of flanking exons, is a crucial step in eukaryotic gene expression. The spliceosome, a macromolecular complex made up of five small nuclear RNAs (snRNAs) and dozens of proteins, assembles on introns via a complex pathway before catalyzing the two transesterification reactions necessary for splicing. All of these steps have the potential to be highly regulated to ensure correct mRNA isoform production for proper cellular function. While Saccharomyces cerevisiae (yeast) has a limited set of intron-containing genes, many of these genes are highly expressed, resulting in a large number of transcripts in a cell being spliced. As a result, splicing regulation is of critical importance for yeast. Just as in humans, yeast splicing can be influenced by protein components of the splicing machinery, structures and properties of the pre-mRNA itself, or by the action of trans-acting factors. It is likely that further analysis of the mechanisms and pathways of splicing regulation in yeast can reveal general principles applicable to other eukaryotes. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Processing > Splicing Regulation/Alternative Splicing.

前核糖核酸剪接,即去除内含子和连接侧翼外显子,是真核生物基因表达的关键步骤。剪接体是由五种小核 RNA(snRNA)和数十种蛋白质组成的大分子复合体,它通过复杂的途径在内含子上组装,然后催化剪接所需的两个酯化反应。所有这些步骤都有可能受到高度调控,以确保产生正确的 mRNA 异构体,从而实现适当的细胞功能。虽然酿酒酵母(酵母)的含内含子基因数量有限,但其中许多基因表达量很高,导致细胞中大量转录本被剪接。因此,剪接调控对酵母至关重要。与人类一样,酵母的剪接也会受到剪接机制的蛋白质成分、前核糖核酸本身的结构和特性或反式作用因子的影响。对酵母剪接调控机制和途径的进一步分析很可能揭示出适用于其他真核生物的一般原则。本文归类于RNA 处理 > 剪接机制 RNA 处理 > 剪接调节/替代剪接。
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引用次数: 0
Deciphering brain cellular and behavioral mechanisms: Insights from single-cell and spatial RNA sequencing. 解密脑细胞和行为机制:单细胞和空间 RNA 测序的启示。
IF 6.4 2区 生物学 Q1 CELL BIOLOGY Pub Date : 2024-07-01 DOI: 10.1002/wrna.1865
Renrui Chen, Pengxing Nie, Jing Wang, Guang-Zhong Wang

The brain is a complex computing system composed of a multitude of interacting neurons. The computational outputs of this system determine the behavior and perception of every individual. Each brain cell expresses thousands of genes that dictate the cell's function and physiological properties. Therefore, deciphering the molecular expression of each cell is of great significance for understanding its characteristics and role in brain function. Additionally, the positional information of each cell can provide crucial insights into their involvement in local brain circuits. In this review, we briefly overview the principles of single-cell RNA sequencing and spatial transcriptomics, the potential issues and challenges in their data processing, and their applications in brain research. We further outline several promising directions in neuroscience that could be integrated with single-cell RNA sequencing, including neurodevelopment, the identification of novel brain microstructures, cognition and behavior, neuronal cell positioning, molecules and cells related to advanced brain functions, sleep-wake cycles/circadian rhythms, and computational modeling of brain function. We believe that the deep integration of these directions with single-cell and spatial RNA sequencing can contribute significantly to understanding the roles of individual cells or cell types in these specific functions, thereby making important contributions to addressing critical questions in those fields. This article is categorized under: RNA Evolution and Genomics > Computational Analyses of RNA RNA in Disease and Development > RNA in Development RNA in Disease and Development > RNA in Disease.

大脑是一个复杂的计算系统,由众多相互作用的神经元组成。该系统的计算输出决定了每个人的行为和感知。每个脑细胞表达数千个基因,这些基因决定了细胞的功能和生理特性。因此,破译每个细胞的分子表达对了解其特征和在大脑功能中的作用具有重要意义。此外,每个细胞的位置信息也能为了解它们在局部脑回路中的参与情况提供重要依据。在这篇综述中,我们简要概述了单细胞 RNA 测序和空间转录组学的原理、数据处理中的潜在问题和挑战,以及它们在脑研究中的应用。我们进一步概述了神经科学中可与单细胞 RNA 测序结合的几个前景广阔的方向,包括神经发育、新型大脑微结构的鉴定、认知与行为、神经元细胞定位、与高级大脑功能相关的分子和细胞、睡眠-觉醒周期/昼夜节律以及大脑功能的计算建模。我们相信,将这些方向与单细胞和空间 RNA 测序深度整合,可大大有助于理解单个细胞或细胞类型在这些特定功能中的作用,从而为解决这些领域的关键问题做出重要贡献。本文归类于RNA 进化与基因组学 > RNA 的计算分析 RNA 在疾病与发育中的作用 > RNA 在发育中的作用 RNA 在疾病与发育中的作用 > RNA 在疾病中的作用。
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引用次数: 0
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Wiley Interdisciplinary Reviews: RNA
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