Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100104
Uma Maheswari Katari, M. Ajitha
A Simple, Accurate and Precise method was developed for the simultaneous estimation of Daunorubicin and Cytarabine in bulk and pharmaceutical dosage form by RPHPLC technique. Chromatogram was run through standard Zorbax C18 (150mm x 4.6 mm, 5μ particle size). Mobile phase containing buffer 0.01N KH2PO4: Acetonitrile taken in the ratio of 60:40 was pumped through column at a flow rate of 1.0 ml/min. Buffer used in this method was 0.01N KH2PO4 buffer. Temperature was set to 30C. Optimized wavelength selected was 240.0 nm. Retention time of Cytarabine and Daunorubicin were found to be 2.325 min and 3.208 nm. % RSD of Daunorubicin and Cytarabine were found to be 0.6 and 0.6 respectively. %Recovery was obtained as 100.30% and 100.33% for Daunorubicin and Cytarabine respectively. LOD, LOQ values obtained from regression equation of Daunorubicin and Cytarabine were 0.09, 0.28 and 0.32, 0.97 respectively. The regression equation of Daunorubicin and Cytarabine is y=39589X+28710 and y=32928X+12457 respectively Retention times were decreased and that run was decreased, so the method was simple and economical that can be adopted in regular Quality control test in Industries.
{"title":"Stability Indicating Method Development and Validation for Determination of Daunorubicin and Cytarabine in Bulk and Pharmaceutical Dosage Form by RP-HPLC","authors":"Uma Maheswari Katari, M. Ajitha","doi":"10.54037/wjps.2022.100104","DOIUrl":"https://doi.org/10.54037/wjps.2022.100104","url":null,"abstract":"A Simple, Accurate and Precise method was developed for the simultaneous estimation of Daunorubicin and Cytarabine in bulk and pharmaceutical dosage form by RPHPLC technique. Chromatogram was run through standard Zorbax C18 (150mm x 4.6 mm, 5μ particle size). Mobile phase containing buffer 0.01N KH2PO4: Acetonitrile taken in the ratio of 60:40 was pumped through column at a flow rate of 1.0 ml/min. Buffer used in this method was 0.01N KH2PO4 buffer. Temperature was set to 30C. Optimized wavelength selected was 240.0 nm. Retention time of Cytarabine and Daunorubicin were found to be 2.325 min and 3.208 nm. % RSD of Daunorubicin and Cytarabine were found to be 0.6 and 0.6 respectively. %Recovery was obtained as 100.30% and 100.33% for Daunorubicin and Cytarabine respectively. LOD, LOQ values obtained from regression equation of Daunorubicin and Cytarabine were 0.09, 0.28 and 0.32, 0.97 respectively. The regression equation of Daunorubicin and Cytarabine is y=39589X+28710 and y=32928X+12457 respectively Retention times were decreased and that run was decreased, so the method was simple and economical that can be adopted in regular Quality control test in Industries.","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"140 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78537662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100702
Saurabh Saklani, Shivam Bisht, Yogesh Joshi
COPDisone of acommonprogressive diseasecharacterized by persistent airflow limitations in the lungs with symptoms of breathlessness, excessive sputum production and chronic cough. This was a prospective, observational study carried out among patients of COPD in a tertiary care hospital to evaluate demographic, etiologicandco-morbidity status.Study was carried out by reviewing prescription of 120 COPD patients,out of which 67.5% were malesand 32.5% were females. The majority of the patients were in age group of 56-60 years (34.16%). Among 120 patients, 65.83% were smokersand48.33% were alcoholics. Etiologic assessment reflected that 68.33% patientbelongs to low socioeconomic status followed by exposure to biomass smoker 8.33%. Out of 120 patients, 53.33% were not havingany other co-morbid condition and the most common co-morbidity found in remaining patients was hypertension 17.5%. This study was concluded to state that such studies were found helpful for existing patient population, publicsand also for scientific researchers.
{"title":"Assessment of Demographic, EtiologicandCo-morbidity Status AmongCOPD Patients","authors":"Saurabh Saklani, Shivam Bisht, Yogesh Joshi","doi":"10.54037/wjps.2022.100702","DOIUrl":"https://doi.org/10.54037/wjps.2022.100702","url":null,"abstract":"COPDisone of acommonprogressive diseasecharacterized by persistent airflow limitations in the lungs with symptoms of breathlessness, excessive sputum production and chronic cough. This was a prospective, observational study carried out among patients of COPD in a tertiary care hospital to evaluate demographic, etiologicandco-morbidity status.Study was carried out by reviewing prescription of 120 COPD patients,out of which 67.5% were malesand 32.5% were females. The majority of the patients were in age group of 56-60 years (34.16%). Among 120 patients, 65.83% were smokersand48.33% were alcoholics. Etiologic assessment reflected that 68.33% patientbelongs to low socioeconomic status followed by exposure to biomass smoker 8.33%. Out of 120 patients, 53.33% were not havingany other co-morbid condition and the most common co-morbidity found in remaining patients was hypertension 17.5%. This study was concluded to state that such studies were found helpful for existing patient population, publicsand also for scientific researchers.","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"40 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89500552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100302
L. S.
Sauropus androgynus (L.) Merr., known traditionally as a multivitamin plant being cultivated in kitchen garden throughout south Indian region. Different organic solvents such as Ethyl acetate, hexane, and methanol were used to extract the bioactive compounds from the fresh air dried plant leaves. Among which ethyl acetate extracted most of the bioactive compounds efficiently. The compounds like tannins, flavonoids, proteins, terpenoids, coumarins and steroids were extracted at varied concentration remain parallel with their bioactive principles. Test organisms used were Escherichia coli, Staphylococcus aureus and Bacillus subtilis showed their sensitiveness with the ethyl acetate extract in vitro. The percentage scavenging activity was also observed to be more with ethyl acetate extract, which was found to increase with concentration.
{"title":"Variation in the efficacy of organic extrants for the phytochemical validation of Sauropus androgynus – a multivitamin plant","authors":"L. S.","doi":"10.54037/wjps.2022.100302","DOIUrl":"https://doi.org/10.54037/wjps.2022.100302","url":null,"abstract":"Sauropus androgynus (L.) Merr., known traditionally as a multivitamin plant being cultivated in kitchen garden throughout south Indian region. Different organic solvents such as Ethyl acetate, hexane, and methanol were used to extract the bioactive compounds from the fresh air dried plant leaves. Among which ethyl acetate extracted most of the bioactive compounds efficiently. The compounds like tannins, flavonoids, proteins, terpenoids, coumarins and steroids were extracted at varied concentration remain parallel with their bioactive principles. Test organisms used were Escherichia coli, Staphylococcus aureus and Bacillus subtilis showed their sensitiveness with the ethyl acetate extract in vitro. The percentage scavenging activity was also observed to be more with ethyl acetate extract, which was found to increase with concentration.","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89736695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100404
Rakhi Sahu, Anurag Pandey, M. Tiwari, N. Mishra, A. Varshney
The genus Citrus contains a vast range of antioxidant metabolites, dietary metabolites and antioxidant polyphenols that protect plants from unfavourable environmental conditions, enhance their tolerance to abiotic and biotic stresses and possess multiple health-promoting effects in humans along with specifically Immunity boosting. Immunity is described as a condition of being able to resist a particular disease especially through preventing the development of a pathogenic microorganism or by counteracting the effects of its products. The immune system is a bodily system that produces the immune response to defend your body from foreign substances, cells and tissues. The immune system includes various parts of the body including the thymus, spleen, lymph nodes, special deposits of lymphoid tissue (such as those in the gastrointestinal tract and bone marrow), macrophages, lymphocytes including the B cells and T cells and antibodies. This review summarizes various citrus fruit and their bioactive components having a positive effect to promote immunity responses in humans body. Additionally, we highlight the health-promoting properties along with Pharmacodynamic and Phytochemical properties of Citrus fruit described in Ayurveda and their potential usage.
{"title":"Effects of citrus fruit and their bioactive components on immunity","authors":"Rakhi Sahu, Anurag Pandey, M. Tiwari, N. Mishra, A. Varshney","doi":"10.54037/wjps.2022.100404","DOIUrl":"https://doi.org/10.54037/wjps.2022.100404","url":null,"abstract":"The genus Citrus contains a vast range of antioxidant metabolites, dietary metabolites and antioxidant polyphenols that protect plants from unfavourable environmental conditions, enhance their tolerance to abiotic and biotic stresses and possess multiple health-promoting effects in humans along with specifically Immunity boosting. Immunity is described as a condition of being able to resist a particular disease especially through preventing the development of a pathogenic microorganism or by counteracting the effects of its products. The immune system is a bodily system that produces the immune response to defend your body from foreign substances, cells and tissues. The immune system includes various parts of the body including the thymus, spleen, lymph nodes, special deposits of lymphoid tissue (such as those in the gastrointestinal tract and bone marrow), macrophages, lymphocytes including the B cells and T cells and antibodies. This review summarizes various citrus fruit and their bioactive components having a positive effect to promote immunity responses in humans body. Additionally, we highlight the health-promoting properties along with Pharmacodynamic and Phytochemical properties of Citrus fruit described in Ayurveda and their potential usage.","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89912417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100207
Kanikella Sivaji, A. Sai, M. Prasad, Sundaresh Ram, N. Vamsi, N. Rahul
{"title":"A Case Report of Pyrogenic Meningitis","authors":"Kanikella Sivaji, A. Sai, M. Prasad, Sundaresh Ram, N. Vamsi, N. Rahul","doi":"10.54037/wjps.2022.100207","DOIUrl":"https://doi.org/10.54037/wjps.2022.100207","url":null,"abstract":"","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89580419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100204
Sweta Sharma, P. Patel, A. Yadav
{"title":"Nutraceuticals aid in managing COVID-19","authors":"Sweta Sharma, P. Patel, A. Yadav","doi":"10.54037/wjps.2022.100204","DOIUrl":"https://doi.org/10.54037/wjps.2022.100204","url":null,"abstract":"","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72647366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100112
Harshini Gandla, M. Ajitha
A simple, Accurate, precise method was developed for the simultaneous estimation of the Netupitant and Palonosetron in Pharmaceutical dosage form. Chromatogram was run through Phenomenex C18 150 mm (4.6 x 150mm, 5μm) Mobile phase containing Buffer 60% 0.01N KH2PO4: 40% Acetonitrile was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 230.0 nm. Retention time of Palonosetron and Netupitant were found to be 2.266 min and 2.945 min. %RSD of the Netupitant and Palonosetron were and found to be 0.8 and 0.8 respectively. %Recovery was obtained as 101.08% and 100.35%for Netupitant and Palonosetron respectively. LOD, LOQ values obtained from regression equations of Netupitant and Palonosetron were 1.27, 3.86 and 0.002, 0.006. respectively. Regression equation of Netupitant is y =18431x + 50471.and y = 13091x + 11.98. of Palonosetron. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
{"title":"Stability Indicating Method Development and Validation for Simultaneous Estimation of the Netupitant and Palonosetron in Bulk and Pharmaceutical Dosage Form by RP-HPLC","authors":"Harshini Gandla, M. Ajitha","doi":"10.54037/wjps.2022.100112","DOIUrl":"https://doi.org/10.54037/wjps.2022.100112","url":null,"abstract":"A simple, Accurate, precise method was developed for the simultaneous estimation of the Netupitant and Palonosetron in Pharmaceutical dosage form. Chromatogram was run through Phenomenex C18 150 mm (4.6 x 150mm, 5μm) Mobile phase containing Buffer 60% 0.01N KH2PO4: 40% Acetonitrile was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 230.0 nm. Retention time of Palonosetron and Netupitant were found to be 2.266 min and 2.945 min. %RSD of the Netupitant and Palonosetron were and found to be 0.8 and 0.8 respectively. %Recovery was obtained as 101.08% and 100.35%for Netupitant and Palonosetron respectively. LOD, LOQ values obtained from regression equations of Netupitant and Palonosetron were 1.27, 3.86 and 0.002, 0.006. respectively. Regression equation of Netupitant is y =18431x + 50471.and y = 13091x + 11.98. of Palonosetron. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91337348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2022.100701
Sankari M, Thamizharasi S, Mariyammal A, Meghana K, Parvatha Raja Kumaran V.S, Chetan A
Rheumatoid arthritis is a chronic systemic inflammation of the synovial joint and progressive destruction of cartilage and bone that may affect many tissues and organs-skin, blood vessels, heart, lungs and muscles but principally attacks the joints, producing a non-suppurative proliferative andinflammatory synovitis that often progresses to destruction of the articular cartilage and ankylosis of the joints. Angiogenesis and microvascular lesions are common features of RA inflammation, which leads to abnormal serum protein infiltration into the synovium. The objective of the study was to determine the preliminary phytochemical constituents, to evaluate acute toxicity studies, In-vitroanti-oxidant studies, In vitroand In-vivoanti-arthritic activity of theroot bark of Spathodea campanulata.. Paw edema was induced in rats by intra plantar injection of 0.1ml of Complete Freund’s Adjuvant (CFA) in left hind paw. The ethanolic extract of Spathodea campanulata (150mg/Kg & 300mg/Kg) and Diclofenac Sodium were orally administered for 28 consecutive days.Percentage inhibition was determined by Protein denaturation method and Membrane Stabilizing Activity Hematological, Histopathology, Radiological Analysis of CFA-induced arthritis in rat was performed. Statisticalsignificant was analyzed by one-way ANOVA followed by Tukey test. Our study concluded that ethanolic extract at the dose of 300 mg/kg showed significant anti-arthritic activity when compared to Diclofenac sodium
{"title":"Evaluation of anti-arthritic activity of Spathodea Campanulata of root bark extract on freund’s adjuvant induced arthritis in rats models","authors":"Sankari M, Thamizharasi S, Mariyammal A, Meghana K, Parvatha Raja Kumaran V.S, Chetan A","doi":"10.54037/wjps.2022.100701","DOIUrl":"https://doi.org/10.54037/wjps.2022.100701","url":null,"abstract":"Rheumatoid arthritis is a chronic systemic inflammation of the synovial joint and progressive destruction of cartilage and bone that may affect many tissues and organs-skin, blood vessels, heart, lungs and muscles but principally attacks the joints, producing a non-suppurative proliferative andinflammatory synovitis that often progresses to destruction of the articular cartilage and ankylosis of the joints. Angiogenesis and microvascular lesions are common features of RA inflammation, which leads to abnormal serum protein infiltration into the synovium. The objective of the study was to determine the preliminary phytochemical constituents, to evaluate acute toxicity studies, In-vitroanti-oxidant studies, In vitroand In-vivoanti-arthritic activity of theroot bark of Spathodea campanulata.. Paw edema was induced in rats by intra plantar injection of 0.1ml of Complete Freund’s Adjuvant (CFA) in left hind paw. The ethanolic extract of Spathodea campanulata (150mg/Kg & 300mg/Kg) and Diclofenac Sodium were orally administered for 28 consecutive days.Percentage inhibition was determined by Protein denaturation method and Membrane Stabilizing Activity Hematological, Histopathology, Radiological Analysis of CFA-induced arthritis in rat was performed. Statisticalsignificant was analyzed by one-way ANOVA followed by Tukey test. Our study concluded that ethanolic extract at the dose of 300 mg/kg showed significant anti-arthritic activity when compared to Diclofenac sodium","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88078440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.54037/wjps.2021.91210
C. Parthiban, Aneesa, M. Sudhakar
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Sitagliptinand Etruglifloxinin pharmaceutical dosage form. The column used was Discovery C18(250mm x 4.6 mm, 5m)in isocratic mode, with mobile phase containing phosphatebufferandacetonitrile (45:55v/v). The buffer is prepared by adding accurately weighed 1.36gm of PotassiumdihyrogenOrtho phosphate in a 1000ml of Volumetric flask add about 900ml of milli-Q water added and degas to sonicate and finally make up the volume with water then pH adjusted to 5.4with dil. Orthophosphoric acid solution.The flow rate was 1.0ml/ min and effluents were monitored at 260nm. The retention times of Sitagliptinand Etruglifloxinwere found to be 2.381min and 3.429min, respectively. The linearity for Sitagliptinand Etruglifloxinwere in the range of 25-150μg/mland 3.75-22.5μg/ml respectively. The recoveries of Sitagliptinand Etruglifloxinwere found to be 99.46to 101.19% and 99.36to 100.99%, respectively. The proposed method was validated and successfully applied to the estimation of Sitagliptinand Etruglifloxinin combined tablet dosage forms.
建立了一种简便、特异、准确的反相高效液相色谱法同时测定西格列汀和依格列星宁制剂剂型的方法。色谱柱为Discovery C18(250mm x 4.6 mm, 5m)，等压柱，流动相为磷酸缓冲液和乙腈(45:55v/v)。缓冲液的制备方法为:在1000ml的容量瓶中加入精确称量的1.36gm磷酸二氢钾，加入约900ml的ml - q水，然后脱气进行超声波处理，最后用水补齐体积，用dil调节pH至5.4。正磷酸溶液。流速1.0ml/ min，在260nm处监测流出物。西格列汀和依trugliloxin保留时间分别为2.381min和3.429min。西格列汀和依曲列酮的线性范围分别为25 ~ 150μg/ml和3.75 ~ 22.5μg/ml。西格列汀和依曲列酮的加样回收率分别为99.46 ~ 101.19%和99.36 ~ 100.99%。该方法经验证并成功应用于西格列汀和依曲列呋辛联用片剂剂型的评价。
{"title":"Analytical method development and validation for simultaneous estimation of sitagliptin and etruglifloxin in bulk and pharmaceutical dosage form by RP-HPLC","authors":"C. Parthiban, Aneesa, M. Sudhakar","doi":"10.54037/wjps.2021.91210","DOIUrl":"https://doi.org/10.54037/wjps.2021.91210","url":null,"abstract":"A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Sitagliptinand Etruglifloxinin pharmaceutical dosage form. The column used was Discovery C18(250mm x 4.6 mm, 5m)in isocratic mode, with mobile phase containing phosphatebufferandacetonitrile (45:55v/v). The buffer is prepared by adding accurately weighed 1.36gm of PotassiumdihyrogenOrtho phosphate in a 1000ml of Volumetric flask add about 900ml of milli-Q water added and degas to sonicate and finally make up the volume with water then pH adjusted to 5.4with dil. Orthophosphoric acid solution.The flow rate was 1.0ml/ min and effluents were monitored at 260nm. The retention times of Sitagliptinand Etruglifloxinwere found to be 2.381min and 3.429min, respectively. The linearity for Sitagliptinand Etruglifloxinwere in the range of 25-150μg/mland 3.75-22.5μg/ml respectively. The recoveries of Sitagliptinand Etruglifloxinwere found to be 99.46to 101.19% and 99.36to 100.99%, respectively. The proposed method was validated and successfully applied to the estimation of Sitagliptinand Etruglifloxinin combined tablet dosage forms.","PeriodicalId":23975,"journal":{"name":"World journal of Pharmacy and pharmaceutical sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80567380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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