Roberto Vázquez-Euan, Estefanía Garibay-Valdez, M. Martínez‐Porchas, L. R. Martínez-Córdova, L. F. Enríquez-Ocaña, R. Vílchez-Vargas, K. Calderón
Microbial communities, mainly those with probiotic characteristics, positively affect the culture of diverse organisms, including shrimp. In this study, three diets containing different types of probiotics were evaluated on the composition and diversity of the microbial community in the water column and gut of Litopenaeus vannamei, as well as on the expression of genes related to the immune system and resistance of the organisms after being challenged with WSSV and Vibrio parahaemolyticus. Results showed that probiotics affected the microbial community structure in water and shrimp. The expression of beta-glucan binding protein and prophenoloxidase increased in the treatments with probiotics compared to the control before and after the challenge. Overall the survival of shrimp was higher in all the treatments than in control. Results suggest that using probiotics in BFT systems could provide an extra benefit for these kinds of systems.
{"title":"Effect of different probiotic diets on microbial gut characterization and gene expression of Litopenaeus vannamei cultivated in BFT system","authors":"Roberto Vázquez-Euan, Estefanía Garibay-Valdez, M. Martínez‐Porchas, L. R. Martínez-Córdova, L. F. Enríquez-Ocaña, R. Vílchez-Vargas, K. Calderón","doi":"10.4194/trjfas21358","DOIUrl":"https://doi.org/10.4194/trjfas21358","url":null,"abstract":"Microbial communities, mainly those with probiotic characteristics, positively affect the culture of diverse organisms, including shrimp. In this study, three diets containing different types of probiotics were evaluated on the composition and diversity of the microbial community in the water column and gut of Litopenaeus vannamei, as well as on the expression of genes related to the immune system and resistance of the organisms after being challenged with WSSV and Vibrio parahaemolyticus. Results showed that probiotics affected the microbial community structure in water and shrimp. The expression of beta-glucan binding protein and prophenoloxidase increased in the treatments with probiotics compared to the control before and after the challenge. Overall the survival of shrimp was higher in all the treatments than in control. Results suggest that using probiotics in BFT systems could provide an extra benefit for these kinds of systems.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44495914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rabia Anwar Rao, A. Qurashi, I. Liaqat, M. Gohar, U. Zafar, Muhammad Amjad Khan, Saraj Bahadur
Waste materials are of major concern around the globe. This study aimed to utilize the scales of fish (Labeo rohita) in microbe-mediated gelatin extraction. Three bacterial isolates were isolated from the fish scales and one bacterial isolate FSW3 showing maximum gelatin yield (61.4 %) was subjected to further analysis. The bacterial culture FSW3 (identified as Staphylococcus spp.) with enhanced production of extracted gelatin was set for physiochemical testing, FTIR analysis, UV-Vis spectroscopy, and stereomicroscopic analysis. The extracted gelatin was successfully utilized in various applications including plant growth promotion under salt stress, anti-adhesion films, firmness of yogurt, biodegradable plastic, and anti-microbial food coatings. Results showed that 0.01 mM gelatin promoted plant growth promotion under salt stress. FSW3 extracted gelatin decreased the 2-fold biofilm-forming ability of E. coli, when exposed to the gelatin-based anti-adhesion films coated on a glass slide and polystyrene sheet. The gelatin showed promising findings as anti-microbial food coatings for tomatoes preservation after 21 days and helpful in the firmness of yogurt. Another advantage of gelatin was casting it into biodegradable plastic. The current study provides a promising approach for fish scales microbe-mediated gelatin extraction and uses it to explore new avenues in biotechnology and microbiology.
{"title":"Utilization of Fish Scales for Microbe-mediated Gelatin Extraction: A New Biotechnology and Microbiology Avenue","authors":"Rabia Anwar Rao, A. Qurashi, I. Liaqat, M. Gohar, U. Zafar, Muhammad Amjad Khan, Saraj Bahadur","doi":"10.4194/trjfas21768","DOIUrl":"https://doi.org/10.4194/trjfas21768","url":null,"abstract":"Waste materials are of major concern around the globe. This study aimed to utilize the scales of fish (Labeo rohita) in microbe-mediated gelatin extraction. Three bacterial isolates were isolated from the fish scales and one bacterial isolate FSW3 showing maximum gelatin yield (61.4 %) was subjected to further analysis. The bacterial culture FSW3 (identified as Staphylococcus spp.) with enhanced production of extracted gelatin was set for physiochemical testing, FTIR analysis, UV-Vis spectroscopy, and stereomicroscopic analysis. The extracted gelatin was successfully utilized in various applications including plant growth promotion under salt stress, anti-adhesion films, firmness of yogurt, biodegradable plastic, and anti-microbial food coatings. Results showed that 0.01 mM gelatin promoted plant growth promotion under salt stress. FSW3 extracted gelatin decreased the 2-fold biofilm-forming ability of E. coli, when exposed to the gelatin-based anti-adhesion films coated on a glass slide and polystyrene sheet. The gelatin showed promising findings as anti-microbial food coatings for tomatoes preservation after 21 days and helpful in the firmness of yogurt. Another advantage of gelatin was casting it into biodegradable plastic. The current study provides a promising approach for fish scales microbe-mediated gelatin extraction and uses it to explore new avenues in biotechnology and microbiology.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45299248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. A. Shaaban, S. Tawfik, Wael El-Tarras, T. El-Sayed Ali
Due to freshwater scarcity, the use of drainage water for aquaculture is a severe challenge in Egypt (one of the cultured tilapia producers worldwide). The present study was conducted, in the highest fish producer governorate (Kafr El-Sheikh). The study aims to assess the water quality of different fish farms fed with different water: agricultural drainage (ADW) and Nile River (Ri-N) water, and examine their suitability for fish culturing. Environmental parameters, nutrients, and metals in the water of fishponds and their sources, were measured. Results revealed that fish farms' waters were brackish (salinity <12 ppt), and slightly alkaline (pH of 8.5). The distribution of DO and BOD between farms was significantly different (p-value<0.05). Metals varied from high Fe levels to below detection limits (Pb and Cd). Feeding water sources showed lower metal content. However, farm fed by Ri-N was enriched by nutrients and attend low metals contents relative to farms fed by ADW, which was verified by the significant statistical differences. Most studied parameters were lower than the acceptable guidelines for fisheries and aquatic life recommended by national and international standards. Overall, the water qualities of fish farms were in good condition and were suitable for tilapia culture.
由于淡水短缺，在埃及（世界养殖罗非鱼生产国之一），使用排水进行水产养殖是一项严峻的挑战。本研究是在鱼类产量最高的省份（Kafr El Sheikh）进行的。该研究旨在评估使用不同水（农业排水（ADW）和尼罗河（Ri-N））喂养的不同渔场的水质，并检查其是否适合鱼类养殖。测量了鱼塘及其水源中的环境参数、营养物质和金属。结果显示，养殖场的水域为微咸（盐度＜12ppt）和微碱性（pH为8.5）。养殖场之间的DO和BOD分布存在显著差异（p值＜0.05）。金属含量从高Fe水平到低于检测限（Pb和Cd）不等。供水水源的金属含量较低。然而，与ADW饲养的农场相比，Ri-N饲养的农场营养丰富，金属含量较低，这一点通过显著的统计差异得到了验证。大多数研究的参数都低于国家和国际标准建议的可接受的渔业和水生生物准则。总的来说，养鱼场的水质状况良好，适合养殖罗非鱼。
{"title":"Are The Water Quality of the Agricultural Drainage and Nile River Suitable for Tilapia Culture? A Case Study from Kafr El-Shaikh's Fish Farms, Egypt","authors":"N. A. Shaaban, S. Tawfik, Wael El-Tarras, T. El-Sayed Ali","doi":"10.4194/trjfas21395","DOIUrl":"https://doi.org/10.4194/trjfas21395","url":null,"abstract":"Due to freshwater scarcity, the use of drainage water for aquaculture is a severe challenge in Egypt (one of the cultured tilapia producers worldwide). The present study was conducted, in the highest fish producer governorate (Kafr El-Sheikh). The study aims to assess the water quality of different fish farms fed with different water: agricultural drainage (ADW) and Nile River (Ri-N) water, and examine their suitability for fish culturing. Environmental parameters, nutrients, and metals in the water of fishponds and their sources, were measured. Results revealed that fish farms' waters were brackish (salinity <12 ppt), and slightly alkaline (pH of 8.5). The distribution of DO and BOD between farms was significantly different (p-value<0.05). Metals varied from high Fe levels to below detection limits (Pb and Cd). Feeding water sources showed lower metal content. However, farm fed by Ri-N was enriched by nutrients and attend low metals contents relative to farms fed by ADW, which was verified by the significant statistical differences. Most studied parameters were lower than the acceptable guidelines for fisheries and aquatic life recommended by national and international standards. Overall, the water qualities of fish farms were in good condition and were suitable for tilapia culture.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42022100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Koffi Bienvenu N’Goran, K. J. Konan, Kouassi Yves Narcisse Kouamé, Annigbé Justin Eyi, K. Kouakou
Hybrids of Coptodon guineensis x Coptodon zillii are omnivorous species with a phytophagous tendency found in most of water bodies of Côte d`Ivoire. This study aims to better understand its reproductive biology and possible influence of macrophytes on the breeding season. A total of 608 fish were caught monthly from September 2017 to August 2019. The overall sex-ratio showed significant predominance of females in Ono (1:1.37; χ² = 7.10; P<0.05) and Kodjoboue (1:1.84; χ² = 28.62; P<0.05) lagoons. The sexual maturity recorded in Ono lagoon for males (148.2 mm, SL) and females (145.3 mm) was slightly higher than that of males (144.4 mm, SL) and females (141.8 mm) in Kodjoboue lagoon. March and August–October (Ono lagoon) and February and July–September (Kodjoboue lagoon) were the most intensive spawning months. The absolute fecundity was higher in Ono lagoon (1472–6116 oocytes) than in Kodjoboue lagoon (1676–4210 oocytes), indicating a better food supply due to the high abundance of macrophytes in Ono lagoon. Oocyte size frequency distributions show oocytes of different sizes, indicating more than one spawning within a reproduction season. These findings might facilitate future studies establishing its potential production and its exploitation and management.
几内亚Coptodon x zillii Coptodon的杂种是杂食性物种，在Côte d ' ivivire的大部分水体中都有植物噬性倾向。本研究旨在更好地了解其生殖生物学以及大型植物对繁殖季节的可能影响。2017年9月至2019年8月，每月共捕获608条鱼。总体性别比显示雌鼠显著优势(1:1.37);χ²= 7.10;P<0.05)和Kodjoboue (1:1.84;χ²= 28.62;P < 0.05)泻湖。小野湖雄性(148.2 mm, SL)和雌性(145.3 mm)的性成熟值略高于Kodjoboue湖雄性(144.4 mm, SL)和雌性(141.8 mm)的性成熟值。3月和8 - 10月(Ono泻湖)以及2月和7 - 9月(Kodjoboue泻湖)是产卵最密集的月份。Ono泻湖的绝对繁殖力(1472-6116个卵母细胞)高于Kodjoboue泻湖(1676-4210个卵母细胞)，表明Ono泻湖的大型植物丰度较高，食物供应较好。卵母细胞大小频率分布显示卵母细胞大小不同，表明在一个繁殖季节内不止一次产卵。这些发现可能有助于未来研究确定其潜在生产和开发管理。
{"title":"Reproductive Biology of the Hybrid Coptodon guineensis x Coptodon zillii within Two Marginal Lagoons Invaded by Macrophytes in South Eastern of Côte d`Ivoire","authors":"Koffi Bienvenu N’Goran, K. J. Konan, Kouassi Yves Narcisse Kouamé, Annigbé Justin Eyi, K. Kouakou","doi":"10.4194/trjfas21680","DOIUrl":"https://doi.org/10.4194/trjfas21680","url":null,"abstract":"Hybrids of Coptodon guineensis x Coptodon zillii are omnivorous species with a phytophagous tendency found in most of water bodies of Côte d`Ivoire. This study aims to better understand its reproductive biology and possible influence of macrophytes on the breeding season. A total of 608 fish were caught monthly from September 2017 to August 2019. The overall sex-ratio showed significant predominance of females in Ono (1:1.37; χ² = 7.10; P<0.05) and Kodjoboue (1:1.84; χ² = 28.62; P<0.05) lagoons. The sexual maturity recorded in Ono lagoon for males (148.2 mm, SL) and females (145.3 mm) was slightly higher than that of males (144.4 mm, SL) and females (141.8 mm) in Kodjoboue lagoon. March and August–October (Ono lagoon) and February and July–September (Kodjoboue lagoon) were the most intensive spawning months. The absolute fecundity was higher in Ono lagoon (1472–6116 oocytes) than in Kodjoboue lagoon (1676–4210 oocytes), indicating a better food supply due to the high abundance of macrophytes in Ono lagoon. Oocyte size frequency distributions show oocytes of different sizes, indicating more than one spawning within a reproduction season. These findings might facilitate future studies establishing its potential production and its exploitation and management.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48280821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tetradesmus obliquus is a unicellular green microalga and considered as a potential source for biotechnological production of pigments such as lutein. No genome-related data is available for T. obliquus that would increase the ability to develop new approaches in biotechnological applications. We present the first transcriptome data for T. obliquus. The aim of this study is to provide a comprehensive transcriptome annotation and identification of conserved genes involved in lutein pigment biosynthesis in Tetradesmus obliquus cells by analyzing pooled RNA-Seq data. Next-Generation Sequencing was applied for the pooled cDNAs library prepared by combining the cell cultures collected from samples exposed to dark and high light intensity conditions. Transcripts were assembled by the de novo assembly approach. Trinotate software was used for functional annotation of assembled transcripts. We also carried out BLAST analysis comparing the transcriptome data against known lutein biosynthesis genes. The 49.15% of the assembled sequences were functionally annotated, providing a total of 21490 unigenes. Our data also revealed the transcript sequences for ten conserved genes required for lutein biosynthesis. The data produced in this study can be used for molecular approaches in biotechnological applications related to T. obliquus, such as increasing the yield of pigment production.
{"title":"De Novo Assembly and Annotation of Microalga Tetradesmus obliquus Transcriptome","authors":"Omer Can Unuvar, E. Unlu","doi":"10.4194/trjfas21341","DOIUrl":"https://doi.org/10.4194/trjfas21341","url":null,"abstract":"Tetradesmus obliquus is a unicellular green microalga and considered as a potential source for biotechnological production of pigments such as lutein. No genome-related data is available for T. obliquus that would increase the ability to develop new approaches in biotechnological applications. We present the first transcriptome data for T. obliquus. The aim of this study is to provide a comprehensive transcriptome annotation and identification of conserved genes involved in lutein pigment biosynthesis in Tetradesmus obliquus cells by analyzing pooled RNA-Seq data. Next-Generation Sequencing was applied for the pooled cDNAs library prepared by combining the cell cultures collected from samples exposed to dark and high light intensity conditions. Transcripts were assembled by the de novo assembly approach. Trinotate software was used for functional annotation of assembled transcripts. We also carried out BLAST analysis comparing the transcriptome data against known lutein biosynthesis genes. The 49.15% of the assembled sequences were functionally annotated, providing a total of 21490 unigenes. Our data also revealed the transcript sequences for ten conserved genes required for lutein biosynthesis. The data produced in this study can be used for molecular approaches in biotechnological applications related to T. obliquus, such as increasing the yield of pigment production.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46483944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to identify Pseudoalteromonas strain OS-9 and evaluate its anti-Vibrio potential. Based on the morphological, biochemical, and 16S rRNA sequence analysis, the strain was identified as Pseudoalteromonas haloplanktis. Experiments designed with Box Behnken showed that cell density and cell-free supernatant activity were simultaneously maximum at 30°C, pH 7, and 90 h. The coefficient values obtained from the response surface methodology regression equations determined that pH was more effective on responses than other physical variables. The cell-free supernatant of P. haloplanktis OS-9 extracted under optimized conditions was only effective on Gram-negative fish pathogenic bacteria and especially on Vibrio. Among Vibrio spp., the strongest inhibitory effect was observed against V. rotiferianus. At the maximum cell density (10.48 OD), the strain OS-9 showed the maximum inhibitory effect against V. rotiferianus (26.42 mm zone diameter). In co-culture assay, the growth of V. rotiferianus was inhibited after 96 h with an initial level of 1.0 × 106 CFU ml-1 by the strain OS-9. These results indicated that P. haloplanktis strain OS-9 could be considered as a potential Vibrio bio-controlling agent.
{"title":"Characterization of Pseudoalteromonas sp. from Aquaculture Environment and Optimization of Fermentation Culture Parameters by RSM-Based Modeling","authors":"Ecren Uzun Yaylacı","doi":"10.4194/trjfas21726","DOIUrl":"https://doi.org/10.4194/trjfas21726","url":null,"abstract":"The aim of the study was to identify Pseudoalteromonas strain OS-9 and evaluate its anti-Vibrio potential. Based on the morphological, biochemical, and 16S rRNA sequence analysis, the strain was identified as Pseudoalteromonas haloplanktis. Experiments designed with Box Behnken showed that cell density and cell-free supernatant activity were simultaneously maximum at 30°C, pH 7, and 90 h. The coefficient values obtained from the response surface methodology regression equations determined that pH was more effective on responses than other physical variables. The cell-free supernatant of P. haloplanktis OS-9 extracted under optimized conditions was only effective on Gram-negative fish pathogenic bacteria and especially on Vibrio. Among Vibrio spp., the strongest inhibitory effect was observed against V. rotiferianus. At the maximum cell density (10.48 OD), the strain OS-9 showed the maximum inhibitory effect against V. rotiferianus (26.42 mm zone diameter). In co-culture assay, the growth of V. rotiferianus was inhibited after 96 h with an initial level of 1.0 × 106 CFU ml-1 by the strain OS-9. These results indicated that P. haloplanktis strain OS-9 could be considered as a potential Vibrio bio-controlling agent.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45071297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel Ofori‐Mensah, M. Yıldız, Vahap Eldem, Cigdem Urku, Çağlayan Kaplan
The present study evaluated fatty acid digestibility, histology, blood biochemistry and gene expression in the gut of juvenile gilthead sea bream, Sparus aurata (initial mean weight, 60.4 ± 4.9 g) fed with iso-proteic (48%) and iso-lipidic (20.7%) diets in which fish oil was partially (60%) or totally (100%) replaced with either camelina or chia oil. Each of the experimental diets was fed to triplicate groups of fish stocked at 10 fish/tank in 150 L tanks for 90 days. Although inclusion of camelina or chia oil altered dietary fatty acid (FA) profile, major differences in digestibility in the individual FAs were not observed. Total replacement of fish oil with camelina or chia oil did not have any effect on blood glucose levels, whereas cholesterol and triglyceride levels were reduced. Results of messenger ribonucleic acid (mRNA) transcription showed elevated levels in the expression of the elongases and desaturases in gilthead seabream fed with diets devoid of fish oil. Fish fed with camelina or chia oil based diets had increased expression in lipolytic and lipogenic genes, resulting in accumulation of lipid droplets in the gut of fish. Histological examination revealed no pathological disorders in the guts of the fish.
{"title":"Effect of dietary inclusion of camelina or chia oil on fatty acid digestibility, histology, blood biochemistry and molecular biomarkers in juvenile gilthead sea bream (Sparus aurata, L.)","authors":"Samuel Ofori‐Mensah, M. Yıldız, Vahap Eldem, Cigdem Urku, Çağlayan Kaplan","doi":"10.4194/trjfas20857","DOIUrl":"https://doi.org/10.4194/trjfas20857","url":null,"abstract":"The present study evaluated fatty acid digestibility, histology, blood biochemistry and gene expression in the gut of juvenile gilthead sea bream, Sparus aurata (initial mean weight, 60.4 ± 4.9 g) fed with iso-proteic (48%) and iso-lipidic (20.7%) diets in which fish oil was partially (60%) or totally (100%) replaced with either camelina or chia oil. Each of the experimental diets was fed to triplicate groups of fish stocked at 10 fish/tank in 150 L tanks for 90 days. Although inclusion of camelina or chia oil altered dietary fatty acid (FA) profile, major differences in digestibility in the individual FAs were not observed. Total replacement of fish oil with camelina or chia oil did not have any effect on blood glucose levels, whereas cholesterol and triglyceride levels were reduced. Results of messenger ribonucleic acid (mRNA) transcription showed elevated levels in the expression of the elongases and desaturases in gilthead seabream fed with diets devoid of fish oil. Fish fed with camelina or chia oil based diets had increased expression in lipolytic and lipogenic genes, resulting in accumulation of lipid droplets in the gut of fish. Histological examination revealed no pathological disorders in the guts of the fish.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48956234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
White spot syndrome virus (WSSV) has caused large economic losses to the aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. Therefore, rapid detection of white spot syndrome virus (WSSV) in shrimp is crucial. This study was conducted to establish a rapid visual detection method for WSSV in shrimp and on the basis of loop-mediated isothermal amplification (LAMP). The color change of the amplification products was observed using a fluorescence amplification curve (with the SYTO-9 fluorescent dye) and LAMP visualization (with calcein), and the method`s specificity, sensitivity, and reproducibility were analyzed. It showed that this method can specifically detect WSSV, with a detection sensitivity of 1 fg/μL and has good reproducibility and reliability. This method was used to analyze cultured shrimp and crayfish samples; among 329 suspected clinical samples, the positive diagnostic rate of WSSV was 27.96%. The infection activity of WSSV was the highest at temperatures of 20–26°C. The LAMP-based method for rapid visual detection of WSSV does not need complex experimental instruments, and an equipment with a stable heat source is sufficient for the reaction. It is easy to use and can be used at shrimp breeding sites.
{"title":"Visual LAMP Method for Detection of White Spot Syndrome Virus in Shrimp and Crayfish","authors":"Chao Ma, Z. Tian, Lu Bai, Lili Yang, Jijuan Cao","doi":"10.4194/trjfas21519","DOIUrl":"https://doi.org/10.4194/trjfas21519","url":null,"abstract":"White spot syndrome virus (WSSV) has caused large economic losses to the aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. Therefore, rapid detection of white spot syndrome virus (WSSV) in shrimp is crucial. This study was conducted to establish a rapid visual detection method for WSSV in shrimp and on the basis of loop-mediated isothermal amplification (LAMP). The color change of the amplification products was observed using a fluorescence amplification curve (with the SYTO-9 fluorescent dye) and LAMP visualization (with calcein), and the method`s specificity, sensitivity, and reproducibility were analyzed. It showed that this method can specifically detect WSSV, with a detection sensitivity of 1 fg/μL and has good reproducibility and reliability. This method was used to analyze cultured shrimp and crayfish samples; among 329 suspected clinical samples, the positive diagnostic rate of WSSV was 27.96%. The infection activity of WSSV was the highest at temperatures of 20–26°C. The LAMP-based method for rapid visual detection of WSSV does not need complex experimental instruments, and an equipment with a stable heat source is sufficient for the reaction. It is easy to use and can be used at shrimp breeding sites.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44108720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Öznur Diler, Öznur Özil, Ikbal Demet Nane, Mevlüt Nazıroğlu, M. Minaz, R. Aslankoç, O. Ozmen, Kamil Atsatan
This study aimed to investigate of bisphenol A (BPA) on histopathological tissue (hepatopancreas and gills), immune ability (lysozyme activity), oxidative stress and antioxidant defence in crayfish (Pontastacus leptodactylus). Crayfish were exposed to BPA to various concentrations (0 (control), and 10, 50, 100 μg/L) for 5 and 20 days in triplicates. Histopathological findings showed moderate to severe hyperemia and inflammatory cell infiltrations, irregular epithelial cell arrangements, degeneration, necrosis and sloughing at the cells, collapse of the hepatopancreas tubules and hyperemia, oedema and sloughing at the epithelial cells of gills. In addition to swelling of gills, accumulation of hemocytes in the hemocoelic space of the gill lamellae relating to dosage were noticed. Antioxidant-related enzyme activities which superoxide dismutase (SOD), Glutathione-S-transferase GST, and glutathione reductase (GR) activity in 10, 50, 100 μg/L BPA group were significantly lower than control at 5 days (p<0.05). Lysozyme activity (LSZ) was no significantly enhanced according to control (p>0.05). These results suggest that the alterations in the antioxidant enzymes and the histological structure of the hepatopancreas, and gill tissue of crayfish can be used as potential biomarkers for risk assessment in aquatic ecosystem.
{"title":"The Effects of Bisphenol A on Oxidative Stress, Antioxidant Defence, Histopathological Alterations and Lysozyme Activity in Narrow-Clawed Crayfish (Pontastacus leptodactylus)","authors":"Öznur Diler, Öznur Özil, Ikbal Demet Nane, Mevlüt Nazıroğlu, M. Minaz, R. Aslankoç, O. Ozmen, Kamil Atsatan","doi":"10.4194/trjfas19877","DOIUrl":"https://doi.org/10.4194/trjfas19877","url":null,"abstract":"This study aimed to investigate of bisphenol A (BPA) on histopathological tissue (hepatopancreas and gills), immune ability (lysozyme activity), oxidative stress and antioxidant defence in crayfish (Pontastacus leptodactylus). Crayfish were exposed to BPA to various concentrations (0 (control), and 10, 50, 100 μg/L) for 5 and 20 days in triplicates. Histopathological findings showed moderate to severe hyperemia and inflammatory cell infiltrations, irregular epithelial cell arrangements, degeneration, necrosis and sloughing at the cells, collapse of the hepatopancreas tubules and hyperemia, oedema and sloughing at the epithelial cells of gills. In addition to swelling of gills, accumulation of hemocytes in the hemocoelic space of the gill lamellae relating to dosage were noticed. Antioxidant-related enzyme activities which superoxide dismutase (SOD), Glutathione-S-transferase GST, and glutathione reductase (GR) activity in 10, 50, 100 μg/L BPA group were significantly lower than control at 5 days (p<0.05). Lysozyme activity (LSZ) was no significantly enhanced according to control (p>0.05). These results suggest that the alterations in the antioxidant enzymes and the histological structure of the hepatopancreas, and gill tissue of crayfish can be used as potential biomarkers for risk assessment in aquatic ecosystem.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41425703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to obtain the spatial-temporal distribution of fish resources together with its changes in sea ranching, and realize the acoustic monitoring and evaluation of fish resource proliferation in sea ranching, the acoustic evaluation technology of fish resources based on the echo statistics method is researched in this paper. Firstly, the simulation is conducted by constructing the fish school echo signal based on the Kirchhoff-ray model, and the simulation results show that the echo statistics method is suitable for acoustic monitoring of fish stocks in sea ranching. Then, the self-developed fish finder was used to survey the national sea ranching demonstration area in the western waters of Furong Island in Shandong Province by navigating in November 2020 and March 2021 respectively. The data obtained from the two voyages were post-processed using the echo statistics method, and the density changes and activities of the fish schools in different times and regions in the surveyed sea were obtained. The analysis results show that the echo statistics method is suitable for the monitoring and evaluation of fishery resources in the sea ranching area.
{"title":"Evaluation of Fish School Resources in Shallow Sea Based on Echo Statistic Method","authors":"Yongxian Wang, Jifeng Si, Yaobin Wang, Xue Li, Xiaoliang Xu, Guoqing Ci","doi":"10.4194/trjfas21344","DOIUrl":"https://doi.org/10.4194/trjfas21344","url":null,"abstract":"In order to obtain the spatial-temporal distribution of fish resources together with its changes in sea ranching, and realize the acoustic monitoring and evaluation of fish resource proliferation in sea ranching, the acoustic evaluation technology of fish resources based on the echo statistics method is researched in this paper. Firstly, the simulation is conducted by constructing the fish school echo signal based on the Kirchhoff-ray model, and the simulation results show that the echo statistics method is suitable for acoustic monitoring of fish stocks in sea ranching. Then, the self-developed fish finder was used to survey the national sea ranching demonstration area in the western waters of Furong Island in Shandong Province by navigating in November 2020 and March 2021 respectively. The data obtained from the two voyages were post-processed using the echo statistics method, and the density changes and activities of the fish schools in different times and regions in the surveyed sea were obtained. The analysis results show that the echo statistics method is suitable for the monitoring and evaluation of fishery resources in the sea ranching area.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49261992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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