The current study focused on green synthesis of iron nanoparticles by Padina pavonica (Pa@FeNPs), removal activity of dye CR (Congo Red) and investigating antioxidant and neurotoxicity activities. The morphological and physical properties of Pa@FeNPs were characterized by FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-Ray Diffractometer), SEM (Scanning Electron Microscopy), EDX (X-Ray Spectroscopy), TEM (Transmission Electron Microscopy) and Zeta potential analyses. XRD and zeta potential analyses revealed a potential of-8.53 mV and formation of cubic crystals with an average dimension of 10.53-20.55 nm. The optimum conditions of pH, temperature, initial dye concentration and adsorbent amount for CR removal were found to be 4, 40°C, 20 ppm and 20 mg respectively. The CR removal percentage using Pa@FeNPs reached a very high efficiency of 95%. Langmuir Isotherm model (R2=0.99, 25°C) were best fitted to our data for evaluating dye adsorption behaviors of the Pa@FeNPs. Metal chelating activity in the aqueous extract of P. pavonica and Pa@FeNPs was found to be 74.71±2.16% and 64.95±0.42%, respectively. Inhibitory concentration 50 (IC50) values for P. pavonica aqueous extract and Pa@FeNPs were found to be 0.128 μg/mL and 400 μg/mL, respectively in the SH-SY5Y (Human neuroblastoma).
{"title":"Biogenic Synthesis of Iron Oxide Nanoparticle Using Padina Pavonica Extract: Application for Photocatalytic Degradation of Congo Red Dye, Neurotoxicity and Antioxidant Activity","authors":"F. Caf","doi":"10.4194/trjfas21398","DOIUrl":"https://doi.org/10.4194/trjfas21398","url":null,"abstract":"The current study focused on green synthesis of iron nanoparticles by Padina pavonica (Pa@FeNPs), removal activity of dye CR (Congo Red) and investigating antioxidant and neurotoxicity activities. The morphological and physical properties of Pa@FeNPs were characterized by FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-Ray Diffractometer), SEM (Scanning Electron Microscopy), EDX (X-Ray Spectroscopy), TEM (Transmission Electron Microscopy) and Zeta potential analyses. XRD and zeta potential analyses revealed a potential of-8.53 mV and formation of cubic crystals with an average dimension of 10.53-20.55 nm. The optimum conditions of pH, temperature, initial dye concentration and adsorbent amount for CR removal were found to be 4, 40°C, 20 ppm and 20 mg respectively. The CR removal percentage using Pa@FeNPs reached a very high efficiency of 95%. Langmuir Isotherm model (R2=0.99, 25°C) were best fitted to our data for evaluating dye adsorption behaviors of the Pa@FeNPs. Metal chelating activity in the aqueous extract of P. pavonica and Pa@FeNPs was found to be 74.71±2.16% and 64.95±0.42%, respectively. Inhibitory concentration 50 (IC50) values for P. pavonica aqueous extract and Pa@FeNPs were found to be 0.128 μg/mL and 400 μg/mL, respectively in the SH-SY5Y (Human neuroblastoma).","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42365287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kamalii, Cheryl Antony, B. Ahilan, A. Uma, E. Prabu
A 60-day indoor growth trial was carried out to investigate the effects of dietary replacement of fish meal (FM) protein with black soldier fly (Hermetia illucens) larvae meal (BSFLM) at graded concentrations on bio-growth parameters, whole-body chemical composition, digestive enzyme activity, muscle-growth-related gene expression and haemato-biochemical responses of goldfish (Carassius auratus) juveniles. Six isonitrogenous and isoenergetic diets were formulated with BSFLM to substitute FM protein at 0 (T0), 20 (T20), 40 (T40), 60 (T60), and 80 (T80), and 100 % (T100). Each diet was randomly assigned to triplicate groups of 20 fish (Mean weight: 2.8±0.3 g) per aquaria were fed twice a day. Goldfish juveniles fed the T60 diet exhibited maximum growth and feed utilization. However, escalating the percentage of fishmeal substitution with BSFLM above 60 percent led to a significant reduction in growth and feed utilization. The protease activity and the haemoglobin (Hb) value were high in fishes fed with the T60 diet. The MyoD was upregulated in fish fed T40 and T60 diets, while the myostatin was downregulated in fish-fed T40 and T60 diets. Thus, the fishmeal protein substituted at 60% with BSFLM with an inclusion level of 201 g/kg of diet will be suitable for the goldfish juveniles.
{"title":"Dietary Protein Replacement of Fish Meal with Black Soldier Fly Larvae Meal: Effects on Growth, Whole-Body Composition, Digestive Enzyme Activity, Muscle-Growth-Related Gene Expression and Haemato-Biochemical Responses of Juvenile Goldfish, Carassius auratus","authors":"A. Kamalii, Cheryl Antony, B. Ahilan, A. Uma, E. Prabu","doi":"10.4194/trjfas21837","DOIUrl":"https://doi.org/10.4194/trjfas21837","url":null,"abstract":"A 60-day indoor growth trial was carried out to investigate the effects of dietary replacement of fish meal (FM) protein with black soldier fly (Hermetia illucens) larvae meal (BSFLM) at graded concentrations on bio-growth parameters, whole-body chemical composition, digestive enzyme activity, muscle-growth-related gene expression and haemato-biochemical responses of goldfish (Carassius auratus) juveniles. Six isonitrogenous and isoenergetic diets were formulated with BSFLM to substitute FM protein at 0 (T0), 20 (T20), 40 (T40), 60 (T60), and 80 (T80), and 100 % (T100). Each diet was randomly assigned to triplicate groups of 20 fish (Mean weight: 2.8±0.3 g) per aquaria were fed twice a day. Goldfish juveniles fed the T60 diet exhibited maximum growth and feed utilization. However, escalating the percentage of fishmeal substitution with BSFLM above 60 percent led to a significant reduction in growth and feed utilization. The protease activity and the haemoglobin (Hb) value were high in fishes fed with the T60 diet. The MyoD was upregulated in fish fed T40 and T60 diets, while the myostatin was downregulated in fish-fed T40 and T60 diets. Thus, the fishmeal protein substituted at 60% with BSFLM with an inclusion level of 201 g/kg of diet will be suitable for the goldfish juveniles.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45032637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Faik Ozan Duzbastilar, Z. Tosunoğlu, Tevfik Ceyhan, M. H. Kaykaç, Celalettin Aydın, Ozlem Gulec, G. Metin
Discard mortality of European anchovy (Engraulis encrasicolus) and European pilchard (Sardina pilchardus) was investigated during commercial purse seine fishing operations off İzmir Bay, Turkey. In total, 6703 anchovy and 2545 sardine individuals were observed for seven days in five survival experiments carried out from March to October 2019. Only in March, fish cages were used and observed by divers. In the other trials, fish were observed in holding tanks; dead fish were collected and any surviving fish were fed. In the study, we compared the mortality of sorted and unsorted fish. The GLM model for European anchovy demonstrated a highly significant category effect (p<0.0001) and a highly significant interaction between length-related mortality and season (CL: season; p<0.0001). There were also significant differences in length-related mortality ratio according to the different categories in both seasons (p<0.0001). However, there was a highly significant interaction between mortality ratio, length, uncrowded category, and spring (p<0.005) for European pilchard. The observations showed that high mortalities occurred in the sorted fish categories of both pelagic species during the experiments.
{"title":"A Variation in the Mortality of European Anchovy and European Pilchard After Sieving and Discarding from a Purse Seine Fishery in the Eastern Mediterranean","authors":"Faik Ozan Duzbastilar, Z. Tosunoğlu, Tevfik Ceyhan, M. H. Kaykaç, Celalettin Aydın, Ozlem Gulec, G. Metin","doi":"10.4194/trjfas21516","DOIUrl":"https://doi.org/10.4194/trjfas21516","url":null,"abstract":"Discard mortality of European anchovy (Engraulis encrasicolus) and European pilchard (Sardina pilchardus) was investigated during commercial purse seine fishing operations off İzmir Bay, Turkey. In total, 6703 anchovy and 2545 sardine individuals were observed for seven days in five survival experiments carried out from March to October 2019. Only in March, fish cages were used and observed by divers. In the other trials, fish were observed in holding tanks; dead fish were collected and any surviving fish were fed. In the study, we compared the mortality of sorted and unsorted fish. The GLM model for European anchovy demonstrated a highly significant category effect (p<0.0001) and a highly significant interaction between length-related mortality and season (CL: season; p<0.0001). There were also significant differences in length-related mortality ratio according to the different categories in both seasons (p<0.0001). However, there was a highly significant interaction between mortality ratio, length, uncrowded category, and spring (p<0.005) for European pilchard. The observations showed that high mortalities occurred in the sorted fish categories of both pelagic species during the experiments.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47622222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mardiansyah Mardiansyah, A. Rijaluddin, Firdaus Ramadhan, Alif Irgi Alfarisi, D. Haribowo
This study on Anthropogenic Litter (AL) was conducted at 5 stations in Situ Gintung, South Tangerang, Indonesia, during the dry and rainy seasons between May to July 2015 and February to April 2016, respectively. The objectives were to determine the correlation between temporally and spatially distributed AL items with gastropods and bioassessment of lake from AL. Generally, the AL consisted of plastic products, rubber, cans, paper, and glass, where plastic bags were discovered more during the two seasons at insignificantly different quantities (p>0.05) and all stations at significantly different amounts (p<0.05). The total number and percentage AL cover with gastropods had a low linear regression value and increased by more than 80% during the rainy season. Meanwhile, the difference in the number of AL was insignificant by season but significant based on the station. Although AL had little effect on gastropods, the number of individual organisms decreased as the number and percentage of cover increased and vice versa. The results suggest that variation of presence of gastropods can be used as a bioassessment inorganic pollution of lake ecosystems.
{"title":"Potential of Gastropods as Bioassessment of Anthropogenic Litter Pollution in Urban Lake","authors":"Mardiansyah Mardiansyah, A. Rijaluddin, Firdaus Ramadhan, Alif Irgi Alfarisi, D. Haribowo","doi":"10.4194/trjfas21266","DOIUrl":"https://doi.org/10.4194/trjfas21266","url":null,"abstract":"This study on Anthropogenic Litter (AL) was conducted at 5 stations in Situ Gintung, South Tangerang, Indonesia, during the dry and rainy seasons between May to July 2015 and February to April 2016, respectively. The objectives were to determine the correlation between temporally and spatially distributed AL items with gastropods and bioassessment of lake from AL. Generally, the AL consisted of plastic products, rubber, cans, paper, and glass, where plastic bags were discovered more during the two seasons at insignificantly different quantities (p>0.05) and all stations at significantly different amounts (p<0.05). The total number and percentage AL cover with gastropods had a low linear regression value and increased by more than 80% during the rainy season. Meanwhile, the difference in the number of AL was insignificant by season but significant based on the station. Although AL had little effect on gastropods, the number of individual organisms decreased as the number and percentage of cover increased and vice versa. The results suggest that variation of presence of gastropods can be used as a bioassessment inorganic pollution of lake ecosystems.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44014749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sibel Doğan Barata, M. Dorucu, N. Sağlam, M. Gurses, O. Otlu
Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomid larval stages are significant pathogens that may exert grave effects on both natural fish and aquaculture populations. Diplostomum species, which use fish as a second intermediate host, is one of the most common trematode species, which affects the fish welfare negatively with the formation of cataracts by settling in the eye. This study is determined the molecular characterization of Diplostomum parasite in Cyprinus carpio and Capoeta umbla for the first time from Turkey, based on the mitochondrial COX1 sequence data. Diplostomum samples were determined as Diplostomum spathaceum according to the phylogenetic trees created in the light of the morphological and COX1 sequence data. Sequence results matched ~98-100% with D. spathaceum as a result of blast analysis. It was defined that this parasite was represented by three different haplotypes in Turkey. As a result of haplotype analysis performed on a total of 162 isolates in GenBank and obtained in this study, 40 polymorphic regions and 58 haplotypes were determined. This haplotype network had arranged within a star-like configuration with a main central haplotype. This shows that the variation within D. spathaceum species is quite high. The mean abundance, mean intensity, and prevalence of the parasite in C. carpio were 8.29 (5.80-11.24), 10.91±9.37 (1-39), and 76%, respectively. In the C umbla, mean abundance, mean intensity, and prevalence were calculated as 11.54 (10.16-12.92), 14.07±16.59 (1-67), and 82%, respectively.
{"title":"Molecular Diversity of Diplostomum spathaceum (Digenea: Diplostomidae) on the Capoeta umbla and Cyprinus carpio (Cypriniformes) Using Mitochondrial DNA Barcode","authors":"Sibel Doğan Barata, M. Dorucu, N. Sağlam, M. Gurses, O. Otlu","doi":"10.4194/trjfas20576","DOIUrl":"https://doi.org/10.4194/trjfas20576","url":null,"abstract":"Diplostomid trematodes (Digenea) include a wide and diverse group of common digeneans. Diplostomid larval stages are significant pathogens that may exert grave effects on both natural fish and aquaculture populations. Diplostomum species, which use fish as a second intermediate host, is one of the most common trematode species, which affects the fish welfare negatively with the formation of cataracts by settling in the eye. This study is determined the molecular characterization of Diplostomum parasite in Cyprinus carpio and Capoeta umbla for the first time from Turkey, based on the mitochondrial COX1 sequence data. Diplostomum samples were determined as Diplostomum spathaceum according to the phylogenetic trees created in the light of the morphological and COX1 sequence data. Sequence results matched ~98-100% with D. spathaceum as a result of blast analysis. It was defined that this parasite was represented by three different haplotypes in Turkey. As a result of haplotype analysis performed on a total of 162 isolates in GenBank and obtained in this study, 40 polymorphic regions and 58 haplotypes were determined. This haplotype network had arranged within a star-like configuration with a main central haplotype. This shows that the variation within D. spathaceum species is quite high. The mean abundance, mean intensity, and prevalence of the parasite in C. carpio were 8.29 (5.80-11.24), 10.91±9.37 (1-39), and 76%, respectively. In the C umbla, mean abundance, mean intensity, and prevalence were calculated as 11.54 (10.16-12.92), 14.07±16.59 (1-67), and 82%, respectively.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42817374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sterlet sturgeon has been identified as a vulnerable species in the IUCN criteria, which is currently lack in most propagation and rehabilitation centers of Iran. This study was conducted with the aim of creating male sterlet sturgeon using gyneogenesis induction without using semen of this sturgeon. Siberian sturgeon, Acipenser baerii sperm was used in this study. To inactivate the sperm DNA, 473 μW/cm2 UV irradiation was used for 90 seconds to maintain sperm motility for the fertility of the ovum. For diploidization of activated eggs, 34°C heat shock was used for 2 minutes, 15 minutes after activation. In this case, fertilization and hatching rate in the gynogenetic progeny were 59.5% and 41.6%, respectively. Sterlet sturgeon gynogenetic progeny was confirmed using microsatellite markers (AFU68 and AFUG9), which had only maternal heritability. Gynogenetic progeny was analyzed by histology of gonads at the age of 17 months. The results showed that both male and female sexes of sterlet sturgeon were created, but these progenies had different sex ratios.
{"title":"Conservation of Vulnerable Sterlet Sturgeon, Acipenser ruthenus Using Artificial Gynogenesis","authors":"M. H. Saber","doi":"10.4194/trjfas20325","DOIUrl":"https://doi.org/10.4194/trjfas20325","url":null,"abstract":"Sterlet sturgeon has been identified as a vulnerable species in the IUCN criteria, which is currently lack in most propagation and rehabilitation centers of Iran. This study was conducted with the aim of creating male sterlet sturgeon using gyneogenesis induction without using semen of this sturgeon. Siberian sturgeon, Acipenser baerii sperm was used in this study. To inactivate the sperm DNA, 473 μW/cm2 UV irradiation was used for 90 seconds to maintain sperm motility for the fertility of the ovum. For diploidization of activated eggs, 34°C heat shock was used for 2 minutes, 15 minutes after activation. In this case, fertilization and hatching rate in the gynogenetic progeny were 59.5% and 41.6%, respectively. Sterlet sturgeon gynogenetic progeny was confirmed using microsatellite markers (AFU68 and AFUG9), which had only maternal heritability. Gynogenetic progeny was analyzed by histology of gonads at the age of 17 months. The results showed that both male and female sexes of sterlet sturgeon were created, but these progenies had different sex ratios.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44570827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. J. Fawole, N. P. Sahu, N. Shamna, A. Adeoye, Vikas Phulia, B. Emikpe
The rising cost of aquafeed ingredients as global aquaculture expands has led researchers to look for other ingredient sources that are not in conflict with human food. In this regard, this study examined the possibility of incorporating detoxified jatropha protein isolate (JP) into the diet of Labeo rohita (rohu) and the effect on the haematology, innate immunity, and organ integrity. A total of 216 fish (3.25 ± 0.02 g/fish) were randomly distributed in 15 tanks and fed for 60 days. The control diet was formulated with soy protein isolate (SP) and gradually replaced with JP at 25%, 50%, 75%, and 100%. The results showed that the red blood cell (RBC) and haemoglobin (Hb) were not significantly influenced (p > 0.05). White blood cell (WBC) counts registered a significant overall effect and followed a quadratic trend (p = 0.001), while the haematocrit value showed overall significant effects (p < 0.05) but no linear or quadratic trends were observed (p > 0.05). Serum lysozyme activity increased with increasing JP level (p = 0.002), but the total immunoglobulin value was significantly higher in fish fed JP 100. The respiratory burst activity, malondialdehyde (MDA) content, superoxide dismutase, and catalase enzyme activities among the various groups did not differ significantly (p > 0.05). Compared to the control, the photomicrograph of the mid-intestine and liver of fish fed a JP-based diet showed no inflammatory or degenerative changes. In summary, the substitution of SP in the diet with JP improved the innate immunity of L. rohita without any negative impact on the blood profile or the histoarchitectural structure of the liver.
{"title":"Effects of Dietary Detoxified Jatropha curcas Protein Isolate on Some Physiological Parameters, Intestine, and Liver Morphology of Labeo rohita Fingerlings","authors":"F. J. Fawole, N. P. Sahu, N. Shamna, A. Adeoye, Vikas Phulia, B. Emikpe","doi":"10.4194/trjfas21623","DOIUrl":"https://doi.org/10.4194/trjfas21623","url":null,"abstract":"The rising cost of aquafeed ingredients as global aquaculture expands has led researchers to look for other ingredient sources that are not in conflict with human food. In this regard, this study examined the possibility of incorporating detoxified jatropha protein isolate (JP) into the diet of Labeo rohita (rohu) and the effect on the haematology, innate immunity, and organ integrity. A total of 216 fish (3.25 ± 0.02 g/fish) were randomly distributed in 15 tanks and fed for 60 days. The control diet was formulated with soy protein isolate (SP) and gradually replaced with JP at 25%, 50%, 75%, and 100%. The results showed that the red blood cell (RBC) and haemoglobin (Hb) were not significantly influenced (p > 0.05). White blood cell (WBC) counts registered a significant overall effect and followed a quadratic trend (p = 0.001), while the haematocrit value showed overall significant effects (p < 0.05) but no linear or quadratic trends were observed (p > 0.05). Serum lysozyme activity increased with increasing JP level (p = 0.002), but the total immunoglobulin value was significantly higher in fish fed JP 100. The respiratory burst activity, malondialdehyde (MDA) content, superoxide dismutase, and catalase enzyme activities among the various groups did not differ significantly (p > 0.05). Compared to the control, the photomicrograph of the mid-intestine and liver of fish fed a JP-based diet showed no inflammatory or degenerative changes. In summary, the substitution of SP in the diet with JP improved the innate immunity of L. rohita without any negative impact on the blood profile or the histoarchitectural structure of the liver.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44103936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sixty scaled carp Cyprinus carpio and sixty mirror carp Cyprinus carpio carpio were raised in fiberglass and concrete tanks. Fish pictures were taken by a digital camera in a lightbox. Image analysis was used to determine length-weight and view area-weight relationships. Length-weight data were fitted to a power equation: (Weight W) = A (Length L)B. R2 ranged from 0.838 to 0.959. Based on the B values, scaled and mirror carps reared in the fiberglass tank showed a negative allometric growth while this was positive for the fish in the concrete tank. For the view area (V)-weight relationship, linear (W = A + BV), and second order polynomial equations were used in addition to the power equation. The R2 values for these equations ranged from 0.916 to 0.995. Skin color was measured by image analysis. Both scaled and mirror carp reared in concrete tanks had L*, a* and b* values significantly higher than those reared in fiberglass tanks (P < .05). Also, the fish reared in fiberglass tanks tended to have less weight for the same view area, compared to those reared in concrete tanks.
{"title":"Image Analysis to Determine Length-Weight and Area-Weight Relationships, and Color Differences in Scaled Carp and Mirror Carp Grown in Fiberglass and Concrete Tanks","authors":"Bahar Gumus, E. Gumus, M. Balaban","doi":"10.4194/trjfas21260","DOIUrl":"https://doi.org/10.4194/trjfas21260","url":null,"abstract":"Sixty scaled carp Cyprinus carpio and sixty mirror carp Cyprinus carpio carpio were raised in fiberglass and concrete tanks. Fish pictures were taken by a digital camera in a lightbox. Image analysis was used to determine length-weight and view area-weight relationships. Length-weight data were fitted to a power equation: (Weight W) = A (Length L)B. R2 ranged from 0.838 to 0.959. Based on the B values, scaled and mirror carps reared in the fiberglass tank showed a negative allometric growth while this was positive for the fish in the concrete tank. For the view area (V)-weight relationship, linear (W = A + BV), and second order polynomial equations were used in addition to the power equation. The R2 values for these equations ranged from 0.916 to 0.995. Skin color was measured by image analysis. Both scaled and mirror carp reared in concrete tanks had L*, a* and b* values significantly higher than those reared in fiberglass tanks (P < .05). Also, the fish reared in fiberglass tanks tended to have less weight for the same view area, compared to those reared in concrete tanks.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45099221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tunde Olowolafe, O. Popoola, O. Fagbenro, Olaronke Olamide Olawusi-Peters
The study was conducted to test the toxicity of Indigofera tinctoria on the antioxidative status and heamatology of Clarias gariepinus. Matured leaves of I. tinctoria were collected, dried, and pulverized. Graded levels of the I. tinctoria leaf powder were weighed and dissolved in 250 ml of water. Data obtained was subjected to one-way to analyse the significant differences between groups and significance level of P<0.05 was used for all tests. Hematological indices indicated a significant (P<0.05) decreasing trend in the red blood cell, haemoglobin and the packed cell volume with increasing concentrations of I. tinctoria while the white blood cell counts increased with increasing concentrations of the toxicant. Stress levels inflicted by the test plant were assessed by estimating the effects on the oxidative stress biomarkers in the kidney, liver, muscle and gill of C. gariepinus. After the 21 days exposure, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and catalase (CAT) were investigated and all increased when compared to control. The findings revealed that the disposal of leaves, and effluent from I. tinctoria could threaten the life and existence of the aquatic organism.
{"title":"Chronic Toxicity of True Indigo (Indigofera tinctoria) Leaf Powder on Antioxidant Status and Haematological Profile of Juveniles African Cat Fish (Clarias gariepinus)","authors":"Tunde Olowolafe, O. Popoola, O. Fagbenro, Olaronke Olamide Olawusi-Peters","doi":"10.4194/trjfas21741","DOIUrl":"https://doi.org/10.4194/trjfas21741","url":null,"abstract":"The study was conducted to test the toxicity of Indigofera tinctoria on the antioxidative status and heamatology of Clarias gariepinus. Matured leaves of I. tinctoria were collected, dried, and pulverized. Graded levels of the I. tinctoria leaf powder were weighed and dissolved in 250 ml of water. Data obtained was subjected to one-way to analyse the significant differences between groups and significance level of P<0.05 was used for all tests. Hematological indices indicated a significant (P<0.05) decreasing trend in the red blood cell, haemoglobin and the packed cell volume with increasing concentrations of I. tinctoria while the white blood cell counts increased with increasing concentrations of the toxicant. Stress levels inflicted by the test plant were assessed by estimating the effects on the oxidative stress biomarkers in the kidney, liver, muscle and gill of C. gariepinus. After the 21 days exposure, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and catalase (CAT) were investigated and all increased when compared to control. The findings revealed that the disposal of leaves, and effluent from I. tinctoria could threaten the life and existence of the aquatic organism.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48464463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Different upwelling index calculations used for oceans were tested by applying to the Aegean Sea and compared with each other. The sea surface temperature (SST) and wind data of ECMWF Reanalysis are used for upwelling index calculations. The decrease in SST is a result of upwelling; however, the parameter that lies behind the phenomenon is the wind stress on the sea surface. The results show that SST and wind-driven upwelling indexes could be roughly used to estimate upwelling phenomena in the Aegean Sea, but it is insufficient for the precise determination of upwelling due to the Aegean Sea`s unique dynamics. To reach a final formula, the `new upwelling index` shall be improved for Aegean Sea in which current data are considered besides SST & wind data.
{"title":"Upwelling Index Calculations in the Aegean Sea","authors":"Emre Tükenmez, H. Altiok","doi":"10.4194/trjfas21621","DOIUrl":"https://doi.org/10.4194/trjfas21621","url":null,"abstract":"Different upwelling index calculations used for oceans were tested by applying to the Aegean Sea and compared with each other. The sea surface temperature (SST) and wind data of ECMWF Reanalysis are used for upwelling index calculations. The decrease in SST is a result of upwelling; however, the parameter that lies behind the phenomenon is the wind stress on the sea surface. The results show that SST and wind-driven upwelling indexes could be roughly used to estimate upwelling phenomena in the Aegean Sea, but it is insufficient for the precise determination of upwelling due to the Aegean Sea`s unique dynamics. To reach a final formula, the `new upwelling index` shall be improved for Aegean Sea in which current data are considered besides SST & wind data.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42787002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}