Intestinal protozoan infection is one of the leading causes of morbidity and mortality worldwide. However, it is difficult to estimate the true problem of protozoal infections because cases are under-reported. This study aimed to identify intestinal protozoa in healthy adults. This study used a categorical descriptive research design and used a multistage random sampling technique. This research was conducted in Pasie Nan Tigo, Koto Tangah, Padang from July 2020 to December 2021 with a total of 61 samples obtained. Furthermore, the samples were examined using the direct stool examination method and with modified Ziehl-Neelsen staining. The results of this study were obtained, from 61 subjects the most age group was 45-49 years (27.9%), the most gender was female (88.5%), and the incidence of intestinal protozoal infection was 3.3% where only Blastocystis hominis found. The vacuole stage and cyst stage were found to be 50%, respectively. All samples examined were single infection. The conclusion of this study is that Blastocystis hominis infection is still found in healthy adults in Pasie Nan Tigo, Koto Tangah, Padang. Therefore, supportive therapy and measures to prevent infection transmission need to be carried out so that the incidence and morbidity rates can be reduced. Keywords: Intestinal protozoa infections, healthy adult
肠道原虫感染是世界范围内发病率和死亡率的主要原因之一。然而，很难估计原生动物感染的真正问题，因为病例报告不足。本研究旨在鉴定健康成人肠道原生动物。本研究采用分类描述性研究设计，采用多阶段随机抽样技术。本研究于2020年7月至2021年12月在巴东的Pasie Nan Tigo, Koto Tangah, Padang进行，共获得61份样本。此外，采用直接粪便检查法和改良Ziehl-Neelsen染色法检查样本。本研究结果显示，61例患者中，45 ~ 49岁年龄组最多(27.9%)，性别以女性最多(88.5%)，肠道原虫感染发生率为3.3%，其中仅发现人芽囊原虫。液泡期和囊肿期分别占50%。所有检测样本均为单一感染。本研究的结论是，在帕西南提戈，Koto Tangah，巴东的健康成年人中仍然存在人芽囊原虫感染。因此，需要采取支持性治疗和预防感染传播的措施，以降低发病率和发病率。关键词:肠道原虫感染;健康成人
{"title":"Identification of Intestinal Protozoa in Adults in Pasie Nan Tigo Sub-Distric Koto Tangah Padang","authors":"Genia Alda Fitria, Nuzulia Irawati, F. Firdawati","doi":"10.19184/ams.v8i3.29697","DOIUrl":"https://doi.org/10.19184/ams.v8i3.29697","url":null,"abstract":"Intestinal protozoan infection is one of the leading causes of morbidity and mortality worldwide. However, it is difficult to estimate the true problem of protozoal infections because cases are under-reported. This study aimed to identify intestinal protozoa in healthy adults. This study used a categorical descriptive research design and used a multistage random sampling technique. This research was conducted in Pasie Nan Tigo, Koto Tangah, Padang from July 2020 to December 2021 with a total of 61 samples obtained. Furthermore, the samples were examined using the direct stool examination method and with modified Ziehl-Neelsen staining. The results of this study were obtained, from 61 subjects the most age group was 45-49 years (27.9%), the most gender was female (88.5%), and the incidence of intestinal protozoal infection was 3.3% where only Blastocystis hominis found. The vacuole stage and cyst stage were found to be 50%, respectively. All samples examined were single infection. The conclusion of this study is that Blastocystis hominis infection is still found in healthy adults in Pasie Nan Tigo, Koto Tangah, Padang. Therefore, supportive therapy and measures to prevent infection transmission need to be carried out so that the incidence and morbidity rates can be reduced. \u0000Keywords: Intestinal protozoa infections, healthy adult","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114356236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic suppurative otitis media (CSOM) or what is known as congek by the local communities, is an ear disease that often occurs in developing countries. Chronic middle ear infection is accompanied by perforation of the tympanic membrane and fluid in the form of watery, clear, thick, even pus that comes out continuously or occurs for 2 months or more. The purpose of this riset was to analyze the relationship between the area of tympanic membrane perforation and hearing threshold value of CSOM patients at RSU Kaliwates Jember. This research is an observational analytic study with a cross sectional approach using secondary data recorded in the medical records of Kaliwates Hospital for 2019-2020. Data analysis using IBM SPSS Pearson test. This study used 15 ears from 12 CSOM patients obtained by a total sampling methode according to inclusion and exclusion criteria. From the analysis, it was found that there was a positive relationship between the area of the tympanic membrane perforation and hearing threshold value (p-value = 0.004) and had a strong degree of correlation (correlation coefficient value was 0.654).
{"title":"The Size of Tympanic Membrane Perforation Area Positively Correlates with The Hearing Threshold of Chronic Suppurative Otitis Media Patient","authors":"Nindya Shinta Rumastika, Syahda Nabilla Aristawidya, Zahrah Febianti","doi":"10.19184/ams.v8i3.23943","DOIUrl":"https://doi.org/10.19184/ams.v8i3.23943","url":null,"abstract":"Chronic suppurative otitis media (CSOM) or what is known as congek by the local communities, is an ear disease that often occurs in developing countries. Chronic middle ear infection is accompanied by perforation of the tympanic membrane and fluid in the form of watery, clear, thick, even pus that comes out continuously or occurs for 2 months or more. The purpose of this riset was to analyze the relationship between the area of tympanic membrane perforation and hearing threshold value of CSOM patients at RSU Kaliwates Jember. This research is an observational analytic study with a cross sectional approach using secondary data recorded in the medical records of Kaliwates Hospital for 2019-2020. Data analysis using IBM SPSS Pearson test. This study used 15 ears from 12 CSOM patients obtained by a total sampling methode according to inclusion and exclusion criteria. From the analysis, it was found that there was a positive relationship between the area of the tympanic membrane perforation and hearing threshold value (p-value = 0.004) and had a strong degree of correlation (correlation coefficient value was 0.654).","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"125 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123556907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Osteoarthritis (OA) is a form of chronic arthritis that mainly attacks the knee joint. The prevalence of knee OA in Indonesia, especially in East Java, is around 27% which is relatively high. Therefore, this study aims to determine the correlation between fatigue and quality of life from knee OA patients at RSD dr. Soebandi Jember. The methodology uses in this study is the analytical observational method with a cross-sectional survey as the research design. For the data, this study uses primary and secondary data. The primary data was obtained through online interviews by phone using a questionnaire instrument, namely Fatigue Assessment Scale (FAS) and World Health Organization Quality of Life (WHOQOL) – BREF. Then, the secondary data was obtained from the medical records of knee OA patients who were undergoing treatment at the RSD dr. Soebandi Jember in the period January 2019 to December 2020. The results of this study showed that the p-value of fatigue with quality of life domain 1 (physical health) was 0.042, domain 2 (psychological) was 0.087, domain 3 (social) was 0.221, and domain 4 (environmental) was 0.426. Therefore, this study concludes that there is a weak relationship between fatigue and quality of life on domain 1 (physical health), with a correlation coefficient of 0.373. Also, there is no relationship between fatigue and quality of life on domain 2 (psychological), domain 3 (social), and domain 4 (environmental) in knee OA patients at RSD dr. Soebandi Jember. Keywords: knee OA, fatigue, quality of life
{"title":"The Correlation between Fatigue and Quality of Life on Knee Osteoarthritis Patient at RSD dr. Soebandi Jember","authors":"Yuli Hermansyah, Marwah Pradani Ridlo, Desie Dwi Wisudanti","doi":"10.19184/ams.v8i3.24683","DOIUrl":"https://doi.org/10.19184/ams.v8i3.24683","url":null,"abstract":"Osteoarthritis (OA) is a form of chronic arthritis that mainly attacks the knee joint. The prevalence of knee OA in Indonesia, especially in East Java, is around 27% which is relatively high. Therefore, this study aims to determine the correlation between fatigue and quality of life from knee OA patients at RSD dr. Soebandi Jember. The methodology uses in this study is the analytical observational method with a cross-sectional survey as the research design. For the data, this study uses primary and secondary data. The primary data was obtained through online interviews by phone using a questionnaire instrument, namely Fatigue Assessment Scale (FAS) and World Health Organization Quality of Life (WHOQOL) – BREF. Then, the secondary data was obtained from the medical records of knee OA patients who were undergoing treatment at the RSD dr. Soebandi Jember in the period January 2019 to December 2020. The results of this study showed that the p-value of fatigue with quality of life domain 1 (physical health) was 0.042, domain 2 (psychological) was 0.087, domain 3 (social) was 0.221, and domain 4 (environmental) was 0.426. Therefore, this study concludes that there is a weak relationship between fatigue and quality of life on domain 1 (physical health), with a correlation coefficient of 0.373. Also, there is no relationship between fatigue and quality of life on domain 2 (psychological), domain 3 (social), and domain 4 (environmental) in knee OA patients at RSD dr. Soebandi Jember. \u0000Keywords: knee OA, fatigue, quality of life","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116787941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lightning injuries can cause multi-organ damage such as burns, ear damage, and nervous system damage. The type of Burns in this trauma consists of linear or flash burns, punctate burns, thermal injuries, Lichtenburg figures, and combination wounds which are generally superficial and heal faster. Lightning strikes also cause ear damage in the form of perforation of the tympanic membrane, bleeding, and micro fractures in the cochlea. The sequelae in lightning injury survivors consist of permanent brain injury, chronic pain syndrome, peripheral neuropathy, and blindness. This case is a man, 20 years old, working as a farmer, referred to the hospital because he was struck by lightning while working in the middle of the rice fields during heavy rain. There were complaints of pain, heat throughout the body, and decreased hearing in both ears in the anamnesis. Physical examination showed a general condition within normal limits. Local status examination found burns as large as 31.5% of the body area, with 2a degree burns (partial thickness) on the chest, back, upper and lower limbs. Examination of the outer and middle ear revealed no abnormalities. The principle of the treatment refers to the electric burns treatment with initial therapy in fluid resuscitation, evaluation of ECG, adequate analgesia, and wound care. The advanced phase of the treatment focuses on treating the burns and evaluating the hearing loss. Adequate care for nine days showed that the wound was epithelialized by 60% of the total wound area and without any other complaints. This case is essential to be studied considering that Indonesia's natural conditions allow for lightning strikes, especially during the rainy season, so lightning is a threat to farmers as a part of the agro-industrial community. Keywords: Lightning injuries, burns, agroindustrial
{"title":"Major Burns After Lightning Strikes at Field: Case Report","authors":"Ulfa Elfiah, W. Akbar","doi":"10.19184/ams.v8i3.31074","DOIUrl":"https://doi.org/10.19184/ams.v8i3.31074","url":null,"abstract":"Lightning injuries can cause multi-organ damage such as burns, ear damage, and nervous system damage. The type of Burns in this trauma consists of linear or flash burns, punctate burns, thermal injuries, Lichtenburg figures, and combination wounds which are generally superficial and heal faster. Lightning strikes also cause ear damage in the form of perforation of the tympanic membrane, bleeding, and micro fractures in the cochlea. The sequelae in lightning injury survivors consist of permanent brain injury, chronic pain syndrome, peripheral neuropathy, and blindness. This case is a man, 20 years old, working as a farmer, referred to the hospital because he was struck by lightning while working in the middle of the rice fields during heavy rain. There were complaints of pain, heat throughout the body, and decreased hearing in both ears in the anamnesis. Physical examination showed a general condition within normal limits. Local status examination found burns as large as 31.5% of the body area, with 2a degree burns (partial thickness) on the chest, back, upper and lower limbs. Examination of the outer and middle ear revealed no abnormalities. The principle of the treatment refers to the electric burns treatment with initial therapy in fluid resuscitation, evaluation of ECG, adequate analgesia, and wound care. The advanced phase of the treatment focuses on treating the burns and evaluating the hearing loss. Adequate care for nine days showed that the wound was epithelialized by 60% of the total wound area and without any other complaints. This case is essential to be studied considering that Indonesia's natural conditions allow for lightning strikes, especially during the rainy season, so lightning is a threat to farmers as a part of the agro-industrial community. \u0000Keywords: Lightning injuries, burns, agroindustrial ","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129384831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious diseases due to opportunistic bacteria and pathogens are still a health problem. Transmission can be prevented by using an alcohol-based antiseptic liquid or naturally. Averrhoa bilimbi L. fruit plants and Cananga odorata flowers contain bioactive compounds that can inhibit bacterial growth. This study aimed to analyze the infusion activity of Averrhoa bilimbi fruit and Cananga odorata flower in single and combined dosage forms against several frequently pathogenic bacteria in vitro. This posttest-only experimental study with a control group design used the paper disc diffusion method. Observation parameters were the diameter of the inhibition zone on the test bacteria after infusion treatment of 50%, 75%, and 100% A.bilimbi fruit and 100% C.odora flowers, as well as 70% alcohol. The results showed that the diameter of the inhibition zone in the combination treatment of A.bilimbi and C.odorata infusion was significantly different from that of the single preparation (p<0.05). The combination of 100% A.bilimbi and 100% C.odorata (ratio 1:1) produced the greatest effect and was equivalent to 70% alcohol. The average inhibition of the combination infusion of Staphylococcus aureus (17.36mm) and Staphylococcus epidermidis (17.57mm) was greater than that of Escherichia coli (14.48 mm) and Salmonella enterica serovar Typhi (14.12mm). In conclusion, the infusion of Averrhoa bilimbi fruit and the Cananga odorata flower had antibacterial activity, and the combined preparation produced a better inhibitory effect than the single preparation. Keywords: antibacterial activity, Averrhoa bilimbi L., Cananga odorata, infusion, single preparation, combination preparation
{"title":"The Antibacterial Activity of Infusion of Averrhoa bilimbi L Fruits and Cananga odorata Flowers against Frequently Pathogenic Bacteria","authors":"L. Budiarti, Erida Wydiamala, Najiya Ulfa","doi":"10.19184/ams.v8i3.29312","DOIUrl":"https://doi.org/10.19184/ams.v8i3.29312","url":null,"abstract":"Infectious diseases due to opportunistic bacteria and pathogens are still a health problem. Transmission can be prevented by using an alcohol-based antiseptic liquid or naturally. Averrhoa bilimbi L. fruit plants and Cananga odorata flowers contain bioactive compounds that can inhibit bacterial growth. This study aimed to analyze the infusion activity of Averrhoa bilimbi fruit and Cananga odorata flower in single and combined dosage forms against several frequently pathogenic bacteria in vitro. This posttest-only experimental study with a control group design used the paper disc diffusion method. Observation parameters were the diameter of the inhibition zone on the test bacteria after infusion treatment of 50%, 75%, and 100% A.bilimbi fruit and 100% C.odora flowers, as well as 70% alcohol. The results showed that the diameter of the inhibition zone in the combination treatment of A.bilimbi and C.odorata infusion was significantly different from that of the single preparation (p<0.05). The combination of 100% A.bilimbi and 100% C.odorata (ratio 1:1) produced the greatest effect and was equivalent to 70% alcohol. The average inhibition of the combination infusion of Staphylococcus aureus (17.36mm) and Staphylococcus epidermidis (17.57mm) was greater than that of Escherichia coli (14.48 mm) and Salmonella enterica serovar Typhi (14.12mm). In conclusion, the infusion of Averrhoa bilimbi fruit and the Cananga odorata flower had antibacterial activity, and the combined preparation produced a better inhibitory effect than the single preparation. \u0000Keywords: antibacterial activity, Averrhoa bilimbi L., Cananga odorata, infusion, single preparation, combination preparation","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131669482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Selly Silfana Liensa, W. S. Utami, P. W. Pralampita, Bagus Hermansyah
Giardia spp. is a common cause of diarrhoeal disease in humans and is also known as a zoonotic agent in humans and animals. There are three risk factors of zoonotic Giardia, the first is individual characteristics, the second is hygiene, and the last is environmental. This study aimed to analyze the hygiene related risk factors for Giardia infection in Sukowono District, Jember. The research design was a cross-sectional study with 52 subjects from Sukowono District, Jember. The study was carried out from December 2020 to April 2021. The sampling method used purposive sampling is a non-probability sample and chi-square test for data analysis. Data was analyzed using SPSS statistics programme. P value < 0,05 was considered statistically significant.The result of microscopic examination of 52 fecal samples found 1.9% of the subjects were infected with Giardia spp. There was no association between hygiene behavior factors with Giardia spp. in Sukowono District, Jember (p>0.05). Data suggest that risk factors may not contribute to Giardia spp. infection. Based on these findings, need to do further research about hygiene related risk factors for Giardia spp. infection in Jember especially Sukowono District. Keywords: Hygiene factor, Giardia infection, zoonosis
{"title":"Hygiene Related Risk Factors for Giardia Infection in Sukowono District, Jember, Indonesia","authors":"Selly Silfana Liensa, W. S. Utami, P. W. Pralampita, Bagus Hermansyah","doi":"10.19184/ams.v8i3.32125","DOIUrl":"https://doi.org/10.19184/ams.v8i3.32125","url":null,"abstract":"Giardia spp. is a common cause of diarrhoeal disease in humans and is also known as a zoonotic agent in humans and animals. There are three risk factors of zoonotic Giardia, the first is individual characteristics, the second is hygiene, and the last is environmental. This study aimed to analyze the hygiene related risk factors for Giardia infection in Sukowono District, Jember. The research design was a cross-sectional study with 52 subjects from Sukowono District, Jember. The study was carried out from December 2020 to April 2021. The sampling method used purposive sampling is a non-probability sample and chi-square test for data analysis. Data was analyzed using SPSS statistics programme. P value < 0,05 was considered statistically significant.The result of microscopic examination of 52 fecal samples found 1.9% of the subjects were infected with Giardia spp. There was no association between hygiene behavior factors with Giardia spp. in Sukowono District, Jember (p>0.05). Data suggest that risk factors may not contribute to Giardia spp. infection. Based on these findings, need to do further research about hygiene related risk factors for Giardia spp. infection in Jember especially Sukowono District. \u0000Keywords: Hygiene factor, Giardia infection, zoonosis","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114895091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acne vulgaris (AV) is a chronic inflammation of pilosebaceous glands. The clinical presentation of AV can be described as an inflammatory lesion and non-inflammatory lesions. The diagnosis of AV is through clinical evaluations. AV was graded according to clinical severity using Lehmann’s criteria. AV predilection most commonly appears on the face, chest, and back. AV causes the greatest burden between 10 to 30 years old and can cause anxiety and decrease self-confidence, instigating decreased quality of life. Acupuncture is a non-pharmacological treatment modality that is performed using filiform needles at acupuncture points. An 18-year-old woman was diagnosed with a moderate degree of AV based on Lehmann's criteria. She received manual acupuncture acupoints with the needles retained for 30 minutes. The treatment was conducted 2 times a week for a total of 12 treatments. After 12 treatments of acupuncture therapy, the degree of AV was reduced from moderate to mild, with an 81.25% reduction in total lesions. Follow-up was carried out 1 month after the treatment, showing that the effect of acupuncture remained. The acupuncture mechanism works directly on acne lesions by improving microcirculation, reducing proinflammatory reactions, and increasing anti-inflammatory, as well as regulating the HPA axis which can reduce stress and improve hormones. Manual acupuncture can be considered a non-pharmacological therapeutic modality to treat AV with minimal side effects.
{"title":"The Effectiveness of Manual Acupuncture Therapy for Acne Vulgaris: A Case Report","authors":"Diana Marsha Fredy, Irma Nareswari","doi":"10.19184/ams.v8i3.31014","DOIUrl":"https://doi.org/10.19184/ams.v8i3.31014","url":null,"abstract":"Acne vulgaris (AV) is a chronic inflammation of pilosebaceous glands. The clinical presentation of AV can be described as an inflammatory lesion and non-inflammatory lesions. The diagnosis of AV is through clinical evaluations. AV was graded according to clinical severity using Lehmann’s criteria. AV predilection most commonly appears on the face, chest, and back. AV causes the greatest burden between 10 to 30 years old and can cause anxiety and decrease self-confidence, instigating decreased quality of life. Acupuncture is a non-pharmacological treatment modality that is performed using filiform needles at acupuncture points. An 18-year-old woman was diagnosed with a moderate degree of AV based on Lehmann's criteria. She received manual acupuncture acupoints with the needles retained for 30 minutes. The treatment was conducted 2 times a week for a total of 12 treatments. After 12 treatments of acupuncture therapy, the degree of AV was reduced from moderate to mild, with an 81.25% reduction in total lesions. Follow-up was carried out 1 month after the treatment, showing that the effect of acupuncture remained. The acupuncture mechanism works directly on acne lesions by improving microcirculation, reducing proinflammatory reactions, and increasing anti-inflammatory, as well as regulating the HPA axis which can reduce stress and improve hormones. Manual acupuncture can be considered a non-pharmacological therapeutic modality to treat AV with minimal side effects.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126239344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Stem cells have been used in regenerative medicine but are so few in the body that they require cell culture. Stem cell culture was performed under normal oxygen tension and passage was carried out until the number of cells was sufficient for therapy. Stem cell cultures under normal oxygen tension do not match the stem cell microenvironment, which can lead to premature senescence. This study aims to determine the association of low oxygen tension with premature senescence of mesenchymal stem cells (MSCs) through inhibition of p21 expression by HIF-1a. Methode: The research method used rabbit bone marrow in New Zealand as a source of MSCs. The results of isolation of MSCs were divided into two groups for cultured on normal and low oxygen tension until 10 passages. Cells were identified using flowcytometry for cd105 and cd34. At early and late passage, the expression of p21 and HIF-1a was examined using immunofluorescence while senescence was examined using β-galactosidase assay. Results: The results showed that in low oxygen cultures HIF-1a expression increased significantly (p <0.05) while p21 expression decreased significantly (p <0.05) as did the β-galactosidase assay. Conclusions: The conclusion of this research is low oxygen tension culture able to decrease premature senescence culture of invitro stem cells mesenchymal through obstacles p21 by HIF-1a. Keywords: p21 expression, HIF-1a expression, late passage, premature senescence.
{"title":"Low Oxygen Tension Inhibits Senescence in Bone Marrow Mesenchymal Stem Cells (BMSCs)","authors":"Candra Bumi","doi":"10.19184/ams.v8i3.33843","DOIUrl":"https://doi.org/10.19184/ams.v8i3.33843","url":null,"abstract":"Background: Stem cells have been used in regenerative medicine but are so few in the body that they require cell culture. Stem cell culture was performed under normal oxygen tension and passage was carried out until the number of cells was sufficient for therapy. Stem cell cultures under normal oxygen tension do not match the stem cell microenvironment, which can lead to premature senescence. This study aims to determine the association of low oxygen tension with premature senescence of mesenchymal stem cells (MSCs) through inhibition of p21 expression by HIF-1a. Methode: The research method used rabbit bone marrow in New Zealand as a source of MSCs. The results of isolation of MSCs were divided into two groups for cultured on normal and low oxygen tension until 10 passages. Cells were identified using flowcytometry for cd105 and cd34. At early and late passage, the expression of p21 and HIF-1a was examined using immunofluorescence while senescence was examined using β-galactosidase assay. Results: The results showed that in low oxygen cultures HIF-1a expression increased significantly (p <0.05) while p21 expression decreased significantly (p <0.05) as did the β-galactosidase assay. Conclusions: The conclusion of this research is low oxygen tension culture able to decrease premature senescence culture of invitro stem cells mesenchymal through obstacles p21 by HIF-1a. \u0000Keywords: p21 expression, HIF-1a expression, late passage, premature senescence.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126850799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vita Kusuma Rahmawati, Mauliya Sri Sukmawati Wahyudi, Dwi Ayu Novinda Sari
Atrial fibrillation (AF) increases 4-5 times incidence of cardiac embolic stroke. In contrast, as many as 70% of AF cases can be detected in stroke within three days of onset, and this can affect the patient's prognosis. This case report describes the causal pathophysiology of AF and acute stroke and identifies the associated risk factors. Case 1 is a 44-year-old woman, acute stroke with acute decrease level of consciousness. The patient came with status epilepticus and a hypertensive emergency. Obtained a history of stroke, uncontrolled hypertension, and myocardial infarction. Initial Score National Institutes of Health Stroke Scale (NIHSS) was 33 and CHA2DS2-VASc was 5. Investigation non-contrast head Computed Tomography (CT) scan showed no bleeding and echocardiography (ECG) showed AF with rapid ventricular response. The patient died after 3 days of treatment. Case 2 is an 83-year-old woman, acute stroke with acute decrease level of consciousness and right motor lateralization. There was a history of hypertension and stroke with left hemiparesis. The initial NIHSS was 28 and CHA2DS2-VASc was 6. Investigations non-contrast head CT scan showed no bleeding and ECG showed AF with normal ventricular response. The patient was discharged from the hospital after being treated for 22 days with NIHSS of 20. Several risk factors for post-stroke AF are elderly, hypertension, subtype of ischemic stroke, prior stroke, and comorbidities such as DM and hypertension. Post-stroke AF can occur because of changes in heart rhythm through autonomic disorders, increased plasma catecholamine levels, and inflammatory reactions. Early Recognition of risk factors, focusing on patients at higher risk of AF detected after stroke becomes important for comprehensive management of stroke with AF. Keywords: acute ischemic stroke, atrial fibrillation, CHA2DS2-VASc score, risk factors.
{"title":"Atrial Fibrillation Detected After Acute Ischemic Stroke","authors":"Vita Kusuma Rahmawati, Mauliya Sri Sukmawati Wahyudi, Dwi Ayu Novinda Sari","doi":"10.19184/ams.v8i3.34235","DOIUrl":"https://doi.org/10.19184/ams.v8i3.34235","url":null,"abstract":"Atrial fibrillation (AF) increases 4-5 times incidence of cardiac embolic stroke. In contrast, as many as 70% of AF cases can be detected in stroke within three days of onset, and this can affect the patient's prognosis. This case report describes the causal pathophysiology of AF and acute stroke and identifies the associated risk factors. Case 1 is a 44-year-old woman, acute stroke with acute decrease level of consciousness. The patient came with status epilepticus and a hypertensive emergency. Obtained a history of stroke, uncontrolled hypertension, and myocardial infarction. Initial Score National Institutes of Health Stroke Scale (NIHSS) was 33 and CHA2DS2-VASc was 5. Investigation non-contrast head Computed Tomography (CT) scan showed no bleeding and echocardiography (ECG) showed AF with rapid ventricular response. The patient died after 3 days of treatment. Case 2 is an 83-year-old woman, acute stroke with acute decrease level of consciousness and right motor lateralization. There was a history of hypertension and stroke with left hemiparesis. The initial NIHSS was 28 and CHA2DS2-VASc was 6. Investigations non-contrast head CT scan showed no bleeding and ECG showed AF with normal ventricular response. The patient was discharged from the hospital after being treated for 22 days with NIHSS of 20. Several risk factors for post-stroke AF are elderly, hypertension, subtype of ischemic stroke, prior stroke, and comorbidities such as DM and hypertension. Post-stroke AF can occur because of changes in heart rhythm through autonomic disorders, increased plasma catecholamine levels, and inflammatory reactions. Early Recognition of risk factors, focusing on patients at higher risk of AF detected after stroke becomes important for comprehensive management of stroke with AF. \u0000Keywords: acute ischemic stroke, atrial fibrillation, CHA2DS2-VASc score, risk factors.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132006606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pipiet Wulandari, Athiyah Fi Ramadani, S. Suryono, Ali Santosa
Lipid metabolic disorder in hyperlipidemia characterized by an increase in the entire lipid profile in the blood. In 2019, the global prevalence of hyperlipidemia was 64.2%. Many patients with diabetes mellitus developed to hyperlipidemia. Diabetes mellitus characterized by hyperglycemia that caused by insulin production defects or decreased insulin resistance. Hyperglycemia in this study resulted from the injection of a streptozotocin dose of 45mg/kgBB intraperitoneally. Hyperglycemia could induced hyperlipidemia. It is due to increased lipid synthesis resulting in overproduction of HMG-CoA and ROS. Moringa leaves contain active substances such as flavonoids and vitamin C that act as antioxidants. This study was conducted to find out the effective dose of moringa leaf extract to lower the total cholesterol levels of male Wistar rats induced by streptozotocin. The dose of moringa leaf extract was divided into 5 groups, namely 62.5 mg/kgBW, 125 mg/kgBW, 250 mg/kgBW, 500 mg/kgBW, and 1000 mg/kgBW. The analysis of the data in this study was a Pearson correlation test that showed p< 0.05. It demonstrated that administration of moringa leaf extract correlated with decreased cholesterol levels of male Wistar rats. The effective dose of moringa leaf extract was calculated using a linear regression test. The equation obtained from the regression test was y= 0.0739x + 153.59, so the dose of moringa leaf extract that effectively lowers the total cholesterol levels of male Wistar rats was 528.96 mg/kgBW. Keywords: hyperlipidemia, diabetes mellitus, cholesterol, Moringa oleifera Lamk., streptozotocin
{"title":"Effective Dose of Moringa Leaf Extract (Moringa oleifera Lamk.) to Descrease Total Cholesterol Levels in Streptozotocin-Induced Male Wistar Rats","authors":"Pipiet Wulandari, Athiyah Fi Ramadani, S. Suryono, Ali Santosa","doi":"10.19184/ams.v8i2.27402","DOIUrl":"https://doi.org/10.19184/ams.v8i2.27402","url":null,"abstract":"Lipid metabolic disorder in hyperlipidemia characterized by an increase in the entire lipid profile in the blood. In 2019, the global prevalence of hyperlipidemia was 64.2%. Many patients with diabetes mellitus developed to hyperlipidemia. Diabetes mellitus characterized by hyperglycemia that caused by insulin production defects or decreased insulin resistance. Hyperglycemia in this study resulted from the injection of a streptozotocin dose of 45mg/kgBB intraperitoneally. Hyperglycemia could induced hyperlipidemia. It is due to increased lipid synthesis resulting in overproduction of HMG-CoA and ROS. Moringa leaves contain active substances such as flavonoids and vitamin C that act as antioxidants. This study was conducted to find out the effective dose of moringa leaf extract to lower the total cholesterol levels of male Wistar rats induced by streptozotocin. The dose of moringa leaf extract was divided into 5 groups, namely 62.5 mg/kgBW, 125 mg/kgBW, 250 mg/kgBW, 500 mg/kgBW, and 1000 mg/kgBW. The analysis of the data in this study was a Pearson correlation test that showed p< 0.05. It demonstrated that administration of moringa leaf extract correlated with decreased cholesterol levels of male Wistar rats. The effective dose of moringa leaf extract was calculated using a linear regression test. The equation obtained from the regression test was y= 0.0739x + 153.59, so the dose of moringa leaf extract that effectively lowers the total cholesterol levels of male Wistar rats was 528.96 mg/kgBW. \u0000Keywords: hyperlipidemia, diabetes mellitus, cholesterol, Moringa oleifera Lamk., streptozotocin","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126628916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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