Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel. Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava
{"title":"The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model","authors":"Yesi Warisman, A. Suryana, Z. Olivia","doi":"10.19184/AMS.V5I2.12076","DOIUrl":"https://doi.org/10.19184/AMS.V5I2.12076","url":null,"abstract":"Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel. \u0000 \u0000Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128953762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus transmitted by Aedes aegyptifemale mosquitoes with four major clinical manifestations, such as high fever, hemorrhage,hepatomegaly, and signs of circulatory failure. Indonesia was reported as the 2nd country with thelargest dengue cases among 30 endemic countries. The larvicidal powder is used as one of thedengue virus vector controllers. This study aims to determine the effect of ethanol extract of smallgooseberry leaves (Phyllantus niruri L.) to the death of Aedes aegypti mosquitoes. This study usedquasi experimental method with post test only controlled group design with 1 positive control group(Temephos), 1 group of negative control (tap water), and 5 treatment groups (0.0625%, 0.125%,0.25% , 0.5% and 1% extract) used 20 larvaes of Aedes aegypti instar III each group. The results of thetreatment group extract with a concentration of 0.5% and 1% obtained 100% dead larvae. The resultof linear regression test showed the effect of meniran leaf extract on the death of larvae Ae. aegyptiwith R2 value of 65.2%. The larvacid activity of ethanol extract leaves meniran against larvae Ae.aegypti instar III was directly proportional to concentration with LC50 of 0.174% with 95% confidenceinterval (0.155-0.195).
{"title":"Aedes aegypti Mosquito Potential Larvacidal of Phyllantus niruri L. Leaves Ethanol Extracts","authors":"M. Hasbullah, Y. Nurdian, Cholis Abrori","doi":"10.19184/AMS.V5I1.6775","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.6775","url":null,"abstract":"Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus transmitted by Aedes aegyptifemale mosquitoes with four major clinical manifestations, such as high fever, hemorrhage,hepatomegaly, and signs of circulatory failure. Indonesia was reported as the 2nd country with thelargest dengue cases among 30 endemic countries. The larvicidal powder is used as one of thedengue virus vector controllers. This study aims to determine the effect of ethanol extract of smallgooseberry leaves (Phyllantus niruri L.) to the death of Aedes aegypti mosquitoes. This study usedquasi experimental method with post test only controlled group design with 1 positive control group(Temephos), 1 group of negative control (tap water), and 5 treatment groups (0.0625%, 0.125%,0.25% , 0.5% and 1% extract) used 20 larvaes of Aedes aegypti instar III each group. The results of thetreatment group extract with a concentration of 0.5% and 1% obtained 100% dead larvae. The resultof linear regression test showed the effect of meniran leaf extract on the death of larvae Ae. aegyptiwith R2 value of 65.2%. The larvacid activity of ethanol extract leaves meniran against larvae Ae.aegypti instar III was directly proportional to concentration with LC50 of 0.174% with 95% confidenceinterval (0.155-0.195).","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124653528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The existence of various industrial centers in Jember Regency has an impact on the high absorption of labor coming from the local community, because most of the production activities are still done by using the power / physical strength of man. Low back pain (LBP) is one of the symptoms of musculoskeletal pain in the lower back, not a disease or diagnosis for a disease. The high risk factor of LBP complaints occurring in industrial workers is the work posture. Patients with chronic LBP complaints may experience affective disorders such as depression and psychosomatic disorders that will lead to a poorer prognosis, while also often experiencing disabilities that implicate the quality of life of patients. This study aims to determine the risk of work posture on worker’s disability index with low back pain complaints in PT Muroco Jember. The type of this research is observational analytic with cross sectional research design which conducted on 31 workers of PT Muroco which fulfill the criteria of inclusion and exclusion. The risk of work posture was obtained by employee observation and completion of the modified quick exposure check questionnaire while the disability index was obtained by filling the modified oswestry low back pain disability questionnaire. The result of the Gamma correlation between the two variables is p = 0,135. It shows that there is no correlation between risk of work posture and worker’s disability index with low back pain complaint in PT Muroco Jember.
{"title":"The Risk of Work Posture Did Not Affect on Worker’s Disability Index with Low Back Pain Complaints in PT Muroco Jember","authors":"Anis Rahmawati, Yohanes Sudarmanto, M. Hasan","doi":"10.19184/AMS.V5I1.6793","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.6793","url":null,"abstract":"The existence of various industrial centers in Jember Regency has an impact on the high absorption of labor coming from the local community, because most of the production activities are still done by using the power / physical strength of man. Low back pain (LBP) is one of the symptoms of musculoskeletal pain in the lower back, not a disease or diagnosis for a disease. The high risk factor of LBP complaints occurring in industrial workers is the work posture. Patients with chronic LBP complaints may experience affective disorders such as depression and psychosomatic disorders that will lead to a poorer prognosis, while also often experiencing disabilities that implicate the quality of life of patients. This study aims to determine the risk of work posture on worker’s disability index with low back pain complaints in PT Muroco Jember. The type of this research is observational analytic with cross sectional research design which conducted on 31 workers of PT Muroco which fulfill the criteria of inclusion and exclusion. The risk of work posture was obtained by employee observation and completion of the modified quick exposure check questionnaire while the disability index was obtained by filling the modified oswestry low back pain disability questionnaire. The result of the Gamma correlation between the two variables is p = 0,135. It shows that there is no correlation between risk of work posture and worker’s disability index with low back pain complaint in PT Muroco Jember.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123841779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jember is one of the districts in East Java Province with the majority of its population working as farmers. Pringgondani Village Farmers in Jember use pesticides when farming. Pesticides contain harmful chemicals. Use of PPE (Personal Protective Equipments) can protect farmers when mixing and spraying pesticides. This study aims to analyze correlation between PPE pesticides usage and health complaints of farmers in Pringgondani Village Sumberjambe District Jember Regency. Cross sectional surveys were conducted on 50 samples. Data were collected by interview using questionnaire. Data were analyzed by chi square test to know correlation between PPE pesticides use (protective clothing, head protection, mask, gloves and boots) and health complaints (headache, increased fatigue, itching and nausea). Chi square test result showed significance 0,043 (p <0,05) where there was a correlation between head protector use and health complaint of respondent with contingency coefficient (r) equal to 0,318. The conclusion is that there is a correlation between the head protection use and health complaints of farmers in Pringgondani Village Sumberjambe District Jember Regency with sufficient correlation. Keywords: PPE, pesticides, health complaints, Farmers, Pringgondani, Jember
{"title":"Analysis of Personal Protective Equipments Pesticides Usage Effects on Health Complaints of Farmers in Pringgondani Village Sumberjambe District Jember Regency","authors":"BJ Azmy As'ady, S. Supangat, Laksmi Indreswari","doi":"10.19184/AMS.V5I1.7901","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.7901","url":null,"abstract":"Jember is one of the districts in East Java Province with the majority of its population working as farmers. Pringgondani Village Farmers in Jember use pesticides when farming. Pesticides contain harmful chemicals. Use of PPE (Personal Protective Equipments) can protect farmers when mixing and spraying pesticides. This study aims to analyze correlation between PPE pesticides usage and health complaints of farmers in Pringgondani Village Sumberjambe District Jember Regency. Cross sectional surveys were conducted on 50 samples. Data were collected by interview using questionnaire. Data were analyzed by chi square test to know correlation between PPE pesticides use (protective clothing, head protection, mask, gloves and boots) and health complaints (headache, increased fatigue, itching and nausea). Chi square test result showed significance 0,043 (p <0,05) where there was a correlation between head protector use and health complaint of respondent with contingency coefficient (r) equal to 0,318. The conclusion is that there is a correlation between the head protection use and health complaints of farmers in Pringgondani Village Sumberjambe District Jember Regency with sufficient correlation. \u0000Keywords: PPE, pesticides, health complaints, Farmers, Pringgondani, Jember","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131676587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bone fracture is a musculoskeletal injury with a high incidence rate. The healing process of the fracture can be inhibited by oxidative stress, which occurs due to Reactive Oxygen Species (ROS) that exceeds the antioxidant capacity in the body that neutralizes it. Antioxidants that have red spinach have the potential to suppress the level of oxidative stress. This study aims to determine the effect of red spinach ethanol extract on the healing process of fracture in male Wistar rats through osteoblast cell count. A sample of 30 male Wistar rats was divided into five groups; negative control group, positive control group, and three groups of red spinach ethanol treatment with doses of 35.4 mg / 150 g body weight (BW), 70.8 mg / 150 gBW, and 141.6 mg / 150gBW induced fracture, splinted, then treated for one week. One Way Anova test results showed a significance of 0.000 (p <0.05) in which there was a significant difference in the osteoblast level between the treatment group and the control group. The result of LSD test between P1 group and P3 group showed significantly different result where the increase of osteoblast cell number was in line with the increase of the dose of red spinach ethanol extract. The conclusion is that red spinach ethanol extract has a positive impact during the fracture healing process with osteoblast cell number parameters. Keywords: Red spinach ethanolic extract, fracture healing process, osteoblast, oxidative stress
{"title":"The Analysis of Osteoblast Cell Number on Femur Fractures Provided Red Spinach Extract (Amaranthus tricolor L.)","authors":"Ferdian Nugroho, Aris Prasetyo, Muhammad Hasan","doi":"10.19184/ams.v5i1.7461","DOIUrl":"https://doi.org/10.19184/ams.v5i1.7461","url":null,"abstract":"Bone fracture is a musculoskeletal injury with a high incidence rate. The healing process of the fracture can be inhibited by oxidative stress, which occurs due to Reactive Oxygen Species (ROS) that exceeds the antioxidant capacity in the body that neutralizes it. Antioxidants that have red spinach have the potential to suppress the level of oxidative stress. This study aims to determine the effect of red spinach ethanol extract on the healing process of fracture in male Wistar rats through osteoblast cell count. A sample of 30 male Wistar rats was divided into five groups; negative control group, positive control group, and three groups of red spinach ethanol treatment with doses of 35.4 mg / 150 g body weight (BW), 70.8 mg / 150 gBW, and 141.6 mg / 150gBW induced fracture, splinted, then treated for one week. One Way Anova test results showed a significance of 0.000 (p <0.05) in which there was a significant difference in the osteoblast level between the treatment group and the control group. The result of LSD test between P1 group and P3 group showed significantly different result where the increase of osteoblast cell number was in line with the increase of the dose of red spinach ethanol extract. The conclusion is that red spinach ethanol extract has a positive impact during the fracture healing process with osteoblast cell number parameters. \u0000Keywords: Red spinach ethanolic extract, fracture healing process, osteoblast, oxidative stress","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115031151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Sofiana, Y W Provisia Marthalita, Khotimah Husnul, M. A. Widodo
Cadmium is a heavy metal that could be found in daily life. This metal has a toxicity, could contaminate the environment, and affect human health. The main aim of this research was to find the effect of low concentration Cadmium exposure in acute time toward HUVECs cell morphology and viability.In a True experimental research with in vitro model using HUVECs cell, HUVECs cell was divided into four groups. One control group (without CdCl2 induction) and three treatment groups with CdCl2 induction with various concentrations, 0,153 µg/L, 1,53 µg/L and 15,3 µg/L. The trial was repeated five times for each group. Cell morphology was observed with an inverted microscope. Cell viability was examined by MTT assay. Data were analyzed using Kruskal Wallis statistical test and continue with the Man Whitney test. Correlation test was using Spearman.Morphology of treatment group HUVECs cell induced by CdCl2 concentration 15,3 µg/L looked significantly different compared with control group (p<0.05). Cell viability on group HUVECs induced by CdCl2 15,3 µg/L significantly different compared with the control group. The correlation test resulted R= -0,665 with probability 0.001 which means the higher concentration of CdCl2 the lower the viability of cells. Cadmium in low concentration induces cell morphology change and reduce cell viability. Keywords: HUVEC, cadmium, cell morphology, cell viability.
{"title":"Analysis of Low–level Cadmium Exposure Effects on HUVECs (Human Umbilical Vein Endothelial Cells) Cell Viability and Morphology","authors":"K. Sofiana, Y W Provisia Marthalita, Khotimah Husnul, M. A. Widodo","doi":"10.19184/AMS.V5I1.9126","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.9126","url":null,"abstract":"Cadmium is a heavy metal that could be found in daily life. This metal has a toxicity, could contaminate the environment, and affect human health. The main aim of this research was to find the effect of low concentration Cadmium exposure in acute time toward HUVECs cell morphology and viability.In a True experimental research with in vitro model using HUVECs cell, HUVECs cell was divided into four groups. One control group (without CdCl2 induction) and three treatment groups with CdCl2 induction with various concentrations, 0,153 µg/L, 1,53 µg/L and 15,3 µg/L. The trial was repeated five times for each group. Cell morphology was observed with an inverted microscope. Cell viability was examined by MTT assay. Data were analyzed using Kruskal Wallis statistical test and continue with the Man Whitney test. Correlation test was using Spearman.Morphology of treatment group HUVECs cell induced by CdCl2 concentration 15,3 µg/L looked significantly different compared with control group (p<0.05). Cell viability on group HUVECs induced by CdCl2 15,3 µg/L significantly different compared with the control group. The correlation test resulted R= -0,665 with probability 0.001 which means the higher concentration of CdCl2 the lower the viability of cells. Cadmium in low concentration induces cell morphology change and reduce cell viability. \u0000Keywords: HUVEC, cadmium, cell morphology, cell viability. \u0000 ","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114183462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Varicose vein (VV) in lower extremities are common diseases in the clinic but there is little concern from clinicians as they are considered mild and low mortality. The complications of VV can be ulcers or worst like Congestive Heart Failure (CHF). Several risk factors that can affect the occurrence of VV are women, age, long standing, overweight, pregnancy multiparity, and hormonal contraception. The general purpose of this research is knowing the relationship between risk factors mentioned above and the prevalence of VV on female laborers of tobacco picker at Ajung District Jember District. This research use observational analytic method with cross sectional study design to 72 people who meet the inclusion criteria by purposive sampling method. The sample were given questionnaire, informed consent, then measured by weight scales and microtoise staturemeter. The diagnosis of VV was obtained from the photos that been consulted to cardiothoracic surgeon at dr. Soebandi Hospital Jember. The result of contingency coefficient C correlation test said that only age has significant relation to VV occurrence states (p = 0,044; r = 0,231; OR = 3,053), while others are not significant (p> 0,05 and r < 0.2). The conclusion of this study is that only the age factor has a statistically significant relationship and the positive correlation with the weak correlation to the occurrence of lower extremities VV on woman laborers of the tobacco picker of Ajung District, Jember Regency. Keywords: Varicose Vein, Lower Extremities
{"title":"Analysis of Varicose Vein in Lower Extremities Risk Factors on Woman Laborers of Tobacco Picker at Ajung District Jember Regency","authors":"Muhammad Akbar, I. Astuti, Desie Dwi Wisudanti","doi":"10.19184/AMS.V5I1.6787","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.6787","url":null,"abstract":"Varicose vein (VV) in lower extremities are common diseases in the clinic but there is little concern from clinicians as they are considered mild and low mortality. The complications of VV can be ulcers or worst like Congestive Heart Failure (CHF). Several risk factors that can affect the occurrence of VV are women, age, long standing, overweight, pregnancy multiparity, and hormonal contraception. The general purpose of this research is knowing the relationship between risk factors mentioned above and the prevalence of VV on female laborers of tobacco picker at Ajung District Jember District. This research use observational analytic method with cross sectional study design to 72 people who meet the inclusion criteria by purposive sampling method. The sample were given questionnaire, informed consent, then measured by weight scales and microtoise staturemeter. The diagnosis of VV was obtained from the photos that been consulted to cardiothoracic surgeon at dr. Soebandi Hospital Jember. The result of contingency coefficient C correlation test said that only age has significant relation to VV occurrence states (p = 0,044; r = 0,231; OR = 3,053), while others are not significant (p> 0,05 and r < 0.2). The conclusion of this study is that only the age factor has a statistically significant relationship and the positive correlation with the weak correlation to the occurrence of lower extremities VV on woman laborers of the tobacco picker of Ajung District, Jember Regency. \u0000Keywords: Varicose Vein, Lower Extremities","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133490856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cigarette smoke was one of the free radical sources for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe because it contains antioxidants substances such as carotene, Vitamin E, isoflavone, and factor II antioxidants. The aim of this study is knowing the influence of giving tempe flour toward the amount of erythrocytes of white male rat (Rattus norvegicus) Wistar strain induced by cigarette smoke. The experiment used a post-test only-control group design method. The samples were white male rats Wistar strain aged of 3 to 4 months as much 30 rats with weight around 180-220 gram. The samples divided into three groups, that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given any treatment, it only got a standard feed, treatment group 1 got treatment and it was induced by cigarette smoke. Furthermore, treatment group 2 got standard feed, it was induced by cigarette smoke and was given tempe. Analysis of data used one-way anova showed the significance p-value = 0,036 that was <α (0,05). The result showed giving tempe flour increase the amounts of erythrocytes of white male rat (Rattus norvegicus) Wistar strain which was induced by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe
{"title":"Tempe Flour Increase The Amount of Erythrocytes of White Male Rat (Rattus norvegicus) Wistar Strain Induced by Cigarette Smoke","authors":"I. M. S. Harsa","doi":"10.19184/AMS.V5I1.9673","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.9673","url":null,"abstract":"Cigarette smoke was one of the free radical sources for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe because it contains antioxidants substances such as carotene, Vitamin E, isoflavone, and factor II antioxidants. The aim of this study is knowing the influence of giving tempe flour toward the amount of erythrocytes of white male rat (Rattus norvegicus) Wistar strain induced by cigarette smoke. The experiment used a post-test only-control group design method. The samples were white male rats Wistar strain aged of 3 to 4 months as much 30 rats with weight around 180-220 gram. The samples divided into three groups, that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given any treatment, it only got a standard feed, treatment group 1 got treatment and it was induced by cigarette smoke. Furthermore, treatment group 2 got standard feed, it was induced by cigarette smoke and was given tempe. Analysis of data used one-way anova showed the significance p-value = 0,036 that was <α (0,05). The result showed giving tempe flour increase the amounts of erythrocytes of white male rat (Rattus norvegicus) Wistar strain which was induced by cigarette smoke. \u0000Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115525153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Electrical burns cause high mortality and morbidity and various complications, although the incidence is only 4% of all burns. Joule heating phenomenon occurs in electric burn patients which cause changes in erythrocyte morphology resulting hypercoagulability that contribute to the formation of thrombus. Changes in erythrocyte morphology may cause tissue oxygenation disorders and decrease in erythrocyte count leading to hypoxia. Until now there have been no reports of changes in erythrocyte morphology and reduction in the number of erythrocytes in burn patients due to electricity. This study aims to observe and analyze morphological and erythrocyte count in rats after electrical exposure of 140 V for 17 seconds. This is a true experimental laboratories study with post-test only control group design. The sample used were 24 rats consisting of one control group (K) and five treatment groups (P). Treatment groups were distinguished by days of blood sampling that is days 0 (P1), 3 (P2), 7 (P3), 10 (P4), and 14 (P5) post electrical exposure of 140 V for 17 seconds. Assessment in erythrocyte morphology was performed using giemsa staining that observed with 1000x magnification while erythrocyte count was performed using improved neubauer counting chamber. One Way Anova showed that there was no significant change in rats erythrocyte count after electrical exposure of 140 V for 17 seconds (p = 0.110). Descriptive analysis showed that there is morphological changes in erythrocyte morphology after electrical exposure of 140 V for 17 seconds, in terms of shape there are more fragmentosit, microsite, tear drops, stomatocytes, and cell target, in terms of size there are more microsite, and in terms of color there are more hypochromic erythrocytes. Keywords: Burn Injury, Erythrocyte Morphology, Erythrocyte Count
{"title":"Analysis of Morphology and Erythrocyte Count in Rat after Electrical Exposure","authors":"Hilda Nur Achfidawati, Ulfa Elfiah, E. N. Sakinah","doi":"10.19184/ams.v5i1.6806","DOIUrl":"https://doi.org/10.19184/ams.v5i1.6806","url":null,"abstract":"Electrical burns cause high mortality and morbidity and various complications, although the incidence is only 4% of all burns. Joule heating phenomenon occurs in electric burn patients which cause changes in erythrocyte morphology resulting hypercoagulability that contribute to the formation of thrombus. Changes in erythrocyte morphology may cause tissue oxygenation disorders and decrease in erythrocyte count leading to hypoxia. Until now there have been no reports of changes in erythrocyte morphology and reduction in the number of erythrocytes in burn patients due to electricity. This study aims to observe and analyze morphological and erythrocyte count in rats after electrical exposure of 140 V for 17 seconds. This is a true experimental laboratories study with post-test only control group design. The sample used were 24 rats consisting of one control group (K) and five treatment groups (P). Treatment groups were distinguished by days of blood sampling that is days 0 (P1), 3 (P2), 7 (P3), 10 (P4), and 14 (P5) post electrical exposure of 140 V for 17 seconds. Assessment in erythrocyte morphology was performed using giemsa staining that observed with 1000x magnification while erythrocyte count was performed using improved neubauer counting chamber. One Way Anova showed that there was no significant change in rats erythrocyte count after electrical exposure of 140 V for 17 seconds (p = 0.110). Descriptive analysis showed that there is morphological changes in erythrocyte morphology after electrical exposure of 140 V for 17 seconds, in terms of shape there are more fragmentosit, microsite, tear drops, stomatocytes, and cell target, in terms of size there are more microsite, and in terms of color there are more hypochromic erythrocytes. \u0000Keywords: Burn Injury, Erythrocyte Morphology, Erythrocyte Count","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124254807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Agustina, D. Mufida, Hanifa Riski A.S, D. K. Dharmawan
Pneumonia is a respiratory tract infection that attacks the pulmonary parenchyma. This disease can be caused by bacteria, one of which is Staphylococcus aureus. Antibiotics have an important role to play in reducing the morbidity and mortality incidence of pneumonia, but currently, the incidence of resistance antibiotics is increased. The purpose of this study was to determine the sensitivity of antibiotics Staphylococcus aureus in pneumatic sputum patients. The design of this study was observational descriptive, using sputum samples from pneumonia patients in Subandi and Paru Jember hospitals in November to December 2018. The identification of bacteria in sputum was continued by testing the sensitivity of bacteria to antibiotics. The antibiotics used in this study were chloramphenicol, gentamicin, amikacin, levofloxacin, ampicillin-sulbactam, cotrimoxazole, and ciprofloxacin — data obtained in the frequency distribution table. Of the ten sputum that was successfully cultured, four Gram-negative bacilli bacteria and six Gram-positive coccus bacteria were obtained. The results approved the six Gram-positive coccus bacteria, detected as Staphylococcus aureus. The antibiotic resistance of Staphylococcus aureus to ampicillin-sulbactam was high. Keywords: antibiotic sensitivity, bacteria, pneumonia
{"title":"Antibiotic Sensitivity Test on Staphylococcus Aureus Detected in Sputum of Patients with Pneumonia Treated in Hospitals","authors":"D. Agustina, D. Mufida, Hanifa Riski A.S, D. K. Dharmawan","doi":"10.19184/AMS.V5I1.9267","DOIUrl":"https://doi.org/10.19184/AMS.V5I1.9267","url":null,"abstract":"Pneumonia is a respiratory tract infection that attacks the pulmonary parenchyma. This disease can be caused by bacteria, one of which is Staphylococcus aureus. Antibiotics have an important role to play in reducing the morbidity and mortality incidence of pneumonia, but currently, the incidence of resistance antibiotics is increased. The purpose of this study was to determine the sensitivity of antibiotics Staphylococcus aureus in pneumatic sputum patients. The design of this study was observational descriptive, using sputum samples from pneumonia patients in Subandi and Paru Jember hospitals in November to December 2018. The identification of bacteria in sputum was continued by testing the sensitivity of bacteria to antibiotics. The antibiotics used in this study were chloramphenicol, gentamicin, amikacin, levofloxacin, ampicillin-sulbactam, cotrimoxazole, and ciprofloxacin — data obtained in the frequency distribution table. Of the ten sputum that was successfully cultured, four Gram-negative bacilli bacteria and six Gram-positive coccus bacteria were obtained. The results approved the six Gram-positive coccus bacteria, detected as Staphylococcus aureus. The antibiotic resistance of Staphylococcus aureus to ampicillin-sulbactam was high. \u0000Keywords: antibiotic sensitivity, bacteria, pneumonia","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"19 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132974428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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