Labyrinthitis is an inflammatory disease of the inner ear perilymphatic cavity and the most common cause of prolonged spontaneous vertigo. Other symptoms are tinnitus and sensorineural hearing loss. Acupuncture is one of the non-pharmacological therapeutic modalities to improve symptoms of labyrinthitis. A 54-year-old woman complained of vertigo that is preceded by tinnitus in both ears since the last 6 months. She was diagnosed with labyrinthitis. Had taken amoxicillin and decadryl for 5 days, betaserc and dimenhydrinate when she felt dizzy, and amlodipine as a routine drug for her hypertension. Physical examination showed vital signs within normal limits, grade 2 obesity, positive Romberg test, and THI score 18. Manual acupuncture was performed at acupoints GV20, GB20, TE17, TE21, SI19, GB2, GB8, TE5, LI4, KI3, PC6 and LR3 with 20 minutes retention. After the first therapy, vertigo complaints disappeared and after 12 treatments (twice a week), frequency and intensity of tinnitus became less, with THI score of 6. Therefore, acupuncture can relieve vertigo and improve tinnitus which can be seen by a decrease of THI score. Keywords: acupuncture, labyrinthitis, tinnitus, vertigo
{"title":"Manual Acupuncture Effect on Vertigo and Tinnitus in Patient with Labyrinthitis: A Case Report","authors":"Cindy Notonegoro, C. Simadibrata","doi":"10.19184/ams.v7i2.23803","DOIUrl":"https://doi.org/10.19184/ams.v7i2.23803","url":null,"abstract":"Labyrinthitis is an inflammatory disease of the inner ear perilymphatic cavity and the most common cause of prolonged spontaneous vertigo. Other symptoms are tinnitus and sensorineural hearing loss. Acupuncture is one of the non-pharmacological therapeutic modalities to improve symptoms of labyrinthitis. A 54-year-old woman complained of vertigo that is preceded by tinnitus in both ears since the last 6 months. She was diagnosed with labyrinthitis. Had taken amoxicillin and decadryl for 5 days, betaserc and dimenhydrinate when she felt dizzy, and amlodipine as a routine drug for her hypertension. Physical examination showed vital signs within normal limits, grade 2 obesity, positive Romberg test, and THI score 18. Manual acupuncture was performed at acupoints GV20, GB20, TE17, TE21, SI19, GB2, GB8, TE5, LI4, KI3, PC6 and LR3 with 20 minutes retention. After the first therapy, vertigo complaints disappeared and after 12 treatments (twice a week), frequency and intensity of tinnitus became less, with THI score of 6. Therefore, acupuncture can relieve vertigo and improve tinnitus which can be seen by a decrease of THI score. \u0000Keywords: acupuncture, labyrinthitis, tinnitus, vertigo","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115240691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Corn silk (Stigma maydis) contains compounds that play a role in wound healing, such as flavonoids, saponins, tannins. This study aims to formulate a gel from corn hair extract and see its activity in healing burns. The test group was divided into four groups: group I (hot metal induction), group II (without extract ), group III (corn hair extract concentration 5%), and group IV (comparison B®), which metal induction. The parameters observed were % wound healing, epithelialization time, and histopathology. The results of the study on the average% of burns healing rate 7, 14 and 21 days, namely group I (27,92%; 62,42%; 100%), group II (35,39%; 60,16%; 100%), group III (44,05%; 63,69%; 100%) and group IV (32,81%; 66,48%; 100%). The mean time of tissue peeling epithelialization groups I, II, and III was on day 16, and group IV was on day 17. For the histopathological results, the mean score of collagen fibers, fibroblast cells, and epithelialization was respectively group I (2,2; 2,7; 2), group II (2,8; 2,9; 2), group III (3,3; 2,9; 2,7), and Group IV (3,9; 3; 3). The results of the two-way ANOVA statistical test on % burn healing (p> 0.05) and one-way ANOVA at the time of epithelialization (p> 0.05) and the histopathological score (p <0.05) showed that group III (the corn hair extract 5 %) could influence the process of healing burns. Keywords: Burns, Corn Silk Extract (Stigma maydis), % Burn Healing, Epithelialization Time, Histopathology
{"title":"Formulation of Gel from Corn Silk Extract (Stigma maydis) and Burns Healing Activity","authors":"N. Putri, N. Nessa, Yoga Ramadhana","doi":"10.19184/ams.v7i2.20287","DOIUrl":"https://doi.org/10.19184/ams.v7i2.20287","url":null,"abstract":"Corn silk (Stigma maydis) contains compounds that play a role in wound healing, such as flavonoids, saponins, tannins. This study aims to formulate a gel from corn hair extract and see its activity in healing burns. The test group was divided into four groups: group I (hot metal induction), group II (without extract ), group III (corn hair extract concentration 5%), and group IV (comparison B®), which metal induction. The parameters observed were % wound healing, epithelialization time, and histopathology. The results of the study on the average% of burns healing rate 7, 14 and 21 days, namely group I (27,92%; 62,42%; 100%), group II (35,39%; 60,16%; 100%), group III (44,05%; 63,69%; 100%) and group IV (32,81%; 66,48%; 100%). The mean time of tissue peeling epithelialization groups I, II, and III was on day 16, and group IV was on day 17. For the histopathological results, the mean score of collagen fibers, fibroblast cells, and epithelialization was respectively group I (2,2; 2,7; 2), group II (2,8; 2,9; 2), group III (3,3; 2,9; 2,7), and Group IV (3,9; 3; 3). The results of the two-way ANOVA statistical test on % burn healing (p> 0.05) and one-way ANOVA at the time of epithelialization (p> 0.05) and the histopathological score (p <0.05) showed that group III (the corn hair extract 5 %) could influence the process of healing burns. \u0000Keywords: Burns, Corn Silk Extract (Stigma maydis), % Burn Healing, Epithelialization Time, Histopathology","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114850646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current nutritional problems are still the world's attention, especially in developing countries, including Indonesia. The Global Nutrition Report shows that Indonesia is a country with three main nutritional problems: wasting, overweight, and stunting (under five) in infants. Stunting has short-term and long-term effects associated with growth and developmental disorders. This study wants to determine the correlation between energy and protein intake of children aged 12-24 months in Plerean, Sumberjambe, Jember. This is an observational research with cross sectional study design. The population of this study were all children aged 12-24 months in Plerean Village, Sumberjambe, Jember. A total of 48 parents/respondents were interviewed about the consumption history of the on the previous day. This is done to determine the energy and protein intake consumed by the research subjects. Measurement of energy and protein intake using 24-hours food recall method and stunting was obtained from secondary data obtained from Puskesmas Sumberjambe. This research used purposive sampling method. The data obtained is then converted into calorie units and processed using a statistical program. The results showed showed 28 subjects were male. Most parents of research subjects earn less than the Minimum Wage District (UMK). A total of 68.75% of subjects had severe deficit in energy intake. A total of 52.08% of subjects had a severe deficit protein. Using Spearman correlation test the correlation of energy intake with the incidence of stunting obtained p value = 0.000 with correlation coefficient -0.589. Then, the relationship of protein intake with the incidence of stunting obtained p = 0.001 with a correlation coefficient of -0.446. So it can be concluded that severe deficit in energy and protein intake correlates with stunting in the study subjects. Keywords: 24-hours food recall, Energy, Protein, Stunting
{"title":"Severe Deficit in Energy and Protein Intake Correlates wih Stunting among Children Aged 12-24 Months in Plerean Sumberjambe Jember","authors":"N. Nurhasanah, D. Rachmawati, I. Sutejo","doi":"10.19184/ams.v7i2.24828","DOIUrl":"https://doi.org/10.19184/ams.v7i2.24828","url":null,"abstract":"The current nutritional problems are still the world's attention, especially in developing countries, including Indonesia. The Global Nutrition Report shows that Indonesia is a country with three main nutritional problems: wasting, overweight, and stunting (under five) in infants. Stunting has short-term and long-term effects associated with growth and developmental disorders. This study wants to determine the correlation between energy and protein intake of children aged 12-24 months in Plerean, Sumberjambe, Jember. This is an observational research with cross sectional study design. The population of this study were all children aged 12-24 months in Plerean Village, Sumberjambe, Jember. A total of 48 parents/respondents were interviewed about the consumption history of the on the previous day. This is done to determine the energy and protein intake consumed by the research subjects. Measurement of energy and protein intake using 24-hours food recall method and stunting was obtained from secondary data obtained from Puskesmas Sumberjambe. This research used purposive sampling method. The data obtained is then converted into calorie units and processed using a statistical program. The results showed showed 28 subjects were male. Most parents of research subjects earn less than the Minimum Wage District (UMK). A total of 68.75% of subjects had severe deficit in energy intake. A total of 52.08% of subjects had a severe deficit protein. Using Spearman correlation test the correlation of energy intake with the incidence of stunting obtained p value = 0.000 with correlation coefficient -0.589. Then, the relationship of protein intake with the incidence of stunting obtained p = 0.001 with a correlation coefficient of -0.446. So it can be concluded that severe deficit in energy and protein intake correlates with stunting in the study subjects. \u0000Keywords: 24-hours food recall, Energy, Protein, Stunting \u0000 ","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124507354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iza Billa Fahmi, E. Sulistyaningsih, Dion Darmawan
Dug well water is often polluted by fecal waste containment such as septic tank and pit latrine system due to its distance. The common pathogenic parasites found in fecal waste containment are E. histolytica and Soil-Transmitted Helminths (STH). The port de entry of both parasites is ingestion of contaminated food or water by fecal materials. This study was aimed to analyze factors affecting the existence of Entamoeba sp. cyst and STH egg on dug well water in Bungatan Village, Situbondo. This observational analytic study used cross-sectional approach, carried out 30 respondents in Bungatan Village, Situbondo. Entamoeba sp. cyst and STH egg were examined using sedimentation method, the distance of fecal waste containment to dug well measured by measuring tape. The characteristic of fecal waste containment and dug well was observed using questionnaires. The results showed 100% fecal containment used pit latrine system, 100% had an incomplete well standard, 66,7% had unstandardized distance with fecal waste containment. Entamoeba sp. cyst and STH egg were not found on all water samples. The soil condition near the dug well (acidity, temperature, moisture, and sunlight intensity) and physical condition (temperature) of dug well water were inappropriate to the development of Entamoeba sp. cyst and STH egg. The dug well's component and its distance to fecal containment did not affect Entamoeba sp. cyst and STH egg existence in all dug well water. The physical soil condition near the dug well and the temperature of dug well water were inappropriate to the development of both parasites. Keywords: Entamoeba sp., Soil Transmitted Helminths, Dug Well, Fecal Waste Containment
{"title":"Analysis of Factors Affecting the Contamination of Entamoeba sp. cyst and Soil-Transmitted Helminths egg on Dug Well Water","authors":"Iza Billa Fahmi, E. Sulistyaningsih, Dion Darmawan","doi":"10.19184/ams.v7i2.22233","DOIUrl":"https://doi.org/10.19184/ams.v7i2.22233","url":null,"abstract":"Dug well water is often polluted by fecal waste containment such as septic tank and pit latrine system due to its distance. The common pathogenic parasites found in fecal waste containment are E. histolytica and Soil-Transmitted Helminths (STH). The port de entry of both parasites is ingestion of contaminated food or water by fecal materials. This study was aimed to analyze factors affecting the existence of Entamoeba sp. cyst and STH egg on dug well water in Bungatan Village, Situbondo. This observational analytic study used cross-sectional approach, carried out 30 respondents in Bungatan Village, Situbondo. Entamoeba sp. cyst and STH egg were examined using sedimentation method, the distance of fecal waste containment to dug well measured by measuring tape. The characteristic of fecal waste containment and dug well was observed using questionnaires. The results showed 100% fecal containment used pit latrine system, 100% had an incomplete well standard, 66,7% had unstandardized distance with fecal waste containment. Entamoeba sp. cyst and STH egg were not found on all water samples. The soil condition near the dug well (acidity, temperature, moisture, and sunlight intensity) and physical condition (temperature) of dug well water were inappropriate to the development of Entamoeba sp. cyst and STH egg. The dug well's component and its distance to fecal containment did not affect Entamoeba sp. cyst and STH egg existence in all dug well water. The physical soil condition near the dug well and the temperature of dug well water were inappropriate to the development of both parasites. \u0000Keywords: Entamoeba sp., Soil Transmitted Helminths, Dug Well, Fecal Waste Containment \u0000","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116686435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isnaini Isnaini, A. Biworo, H. Khatimah, Khusnan Mustofa Gufron, Shafa Rahmani Puteri
Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) is a peat swamp forest plant. This plant is used to treat respiratory diseases, colitis, sore throat, itchy skin, diarrhea, and dizziness. This study aimed to analyze the antibacterial and antifungal activity of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit against Escherichia coli and Candida albicans bacteria. This research is a pretest-posttest research with control group design and consists of 3 stages of research. The first stage is the phytochemical screening test, the second stage determines the minimum inhibitory concentration (MIC) and the third determines the minimum bactericidal concentration (MBC). The results showed that the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit contained terpenoids, flavonoids, phenols, saponins, quinones, steroids, tannins, while alkaloid compounds only found in the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit. The MIC and MBC values of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower methanol extract against E. coli were 1.67 and 2.083 mg/mL, respectively, while the MIC and MBC values of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower against C. albicans were 3.125 and 25 mg/mL. The MIC and MBC values of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against E. coli have same value were 3.334 mg/mL, while the MIC and MBC values of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against C. albicans were 3.125 and 12.5 mg/mL, respectively. Keywords: Galam antibacterial, antifungal, Escherichia coli, Candida albicans
{"title":"Antibacterial and Antifungal Activity of Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) Extract against E. coli bacteria and C. albicans fungi","authors":"Isnaini Isnaini, A. Biworo, H. Khatimah, Khusnan Mustofa Gufron, Shafa Rahmani Puteri","doi":"10.19184/ams.v7i2.23467","DOIUrl":"https://doi.org/10.19184/ams.v7i2.23467","url":null,"abstract":"Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) is a peat swamp forest plant. This plant is used to treat respiratory diseases, colitis, sore throat, itchy skin, diarrhea, and dizziness. This study aimed to analyze the antibacterial and antifungal activity of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit against Escherichia coli and Candida albicans bacteria. This research is a pretest-posttest research with control group design and consists of 3 stages of research. The first stage is the phytochemical screening test, the second stage determines the minimum inhibitory concentration (MIC) and the third determines the minimum bactericidal concentration (MBC). The results showed that the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit contained terpenoids, flavonoids, phenols, saponins, quinones, steroids, tannins, while alkaloid compounds only found in the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit. The MIC and MBC values of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower methanol extract against E. coli were 1.67 and 2.083 mg/mL, respectively, while the MIC and MBC values of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower against C. albicans were 3.125 and 25 mg/mL. The MIC and MBC values of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against E. coli have same value were 3.334 mg/mL, while the MIC and MBC values of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against C. albicans were 3.125 and 12.5 mg/mL, respectively. \u0000Keywords: Galam antibacterial, antifungal, Escherichia coli, Candida albicans","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127932172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stunting is one of the remaining health issues in Indonesia and its prevalence was still high in the last decades. There are two types of growth charts that can be used in recording the child's growth: growth standards and growth references. In recent times, the selection of the suitable growth charts has become a subject of discussion in many countries. The objective of this study is to describe the prevalence of stunting according to World Health Organization Child Growth Standards (WHOCGS) and Indonesian National Growth Reference Charts (INGRC) of children under five from the Blega sub-district. The secondary data from 2884 children were collected recapitulation of the 'Bulan Timbang' program in February 2020. Z-score of length/height-for-age was plotted according to WHOCGS and INGRC. The result showed that the prevalence of stunting were lower for the INGRC than WHOCGS (5.83% and 11.17%, with p-value <0.001). There was an advantage and disadvantage when using both two growth charts. Further research is still needed to support the result of this study. Keywords: Stunting, World Health Organization Child Growth Standards, Indonesian National Growth Reference Charts
{"title":"Prevalence of Stunting According to WHO Growth Standards and Indonesian Growth Reference Charts: A Descriptive Study from Blega Sub-District","authors":"N. Hilmy, Maitsa' Fatharani","doi":"10.19184/ams.v7i2.23868","DOIUrl":"https://doi.org/10.19184/ams.v7i2.23868","url":null,"abstract":"Stunting is one of the remaining health issues in Indonesia and its prevalence was still high in the last decades. There are two types of growth charts that can be used in recording the child's growth: growth standards and growth references. In recent times, the selection of the suitable growth charts has become a subject of discussion in many countries. The objective of this study is to describe the prevalence of stunting according to World Health Organization Child Growth Standards (WHOCGS) and Indonesian National Growth Reference Charts (INGRC) of children under five from the Blega sub-district. The secondary data from 2884 children were collected recapitulation of the 'Bulan Timbang' program in February 2020. Z-score of length/height-for-age was plotted according to WHOCGS and INGRC. The result showed that the prevalence of stunting were lower for the INGRC than WHOCGS (5.83% and 11.17%, with p-value <0.001). There was an advantage and disadvantage when using both two growth charts. Further research is still needed to support the result of this study. \u0000Keywords: Stunting, World Health Organization Child Growth Standards, Indonesian National Growth Reference Charts","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128855588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bercak darah dapat ditemukan di tempat kejadian perkara (TKP) pada banyak kasus tindak kekerasan. Asam deoksiribonukleat (DNA) pada darah dapat digunakan sebagai data primer untuk proses identifikasi akan tetapi bercak darah di TKP berisiko rusak akibat pajanan tanah dan ultraviolet. Tujuan utama dari penelitian ini adalah untuk mempelajari efek kombinasi dari pajanan sinar ultraviolet-C dan tanah terhadap kualitas dan kuantitas DNA pada bercak darah. Sebanyak 20 gelas berisi 200 gram tanah ditetesi 900µl darah dan diberikan pajanan sinar ultraviolet-C dalam tiga kelompok berdasarkan durasi pajanan yakni satu hari, tiga hari, dan lima hari. Satu kelompok digunakan sebagai kontrol. Ekstraksi DNA dilakukan menggunakan DNAZol dilanjutkan dengan pengukuran spektrofotometri untuk mengetahui kualitas dan kuantitas DNA. Peningkatan konsentrasi DNA dapat diamati yaitu 681,1 pada hari pertama menjadi 1274,7 pada hari ketiga dan mulai menurun menjadi 1090,6 pada hari kelima, sedangkan kemurnian DNA terus menurun secara konstan seiring dengan meningkatnya durasi pajanan. Penelitian ini menunjukkan bahwa pajanan sinar ultraviolet-C dan tanah menyebabkan degradasi molekul DNA menjadi fragmen-fragmen molekul yang lebih kecil sehingga terjadi peningkatan kuantitas DNA yang disertai penurunan kualitas DNA. Penurunan kualitas DNA dapat mempersulit proses identifikasi sehingga isolasi DNA sampel pada tanah terbuka yang terpajan matahari harus dilakukan sesegera mungkin. Kata Kunci: DNA, darah, tanah, ultraviolet C, patologi forensik
{"title":"DNA Quality and Quantity on Blood Spot Post Soil and Ultraviolet-C Exposure","authors":"M. A. Jauhani, Sheilla Rachmania","doi":"10.19184/AMS.V6I3.19937","DOIUrl":"https://doi.org/10.19184/AMS.V6I3.19937","url":null,"abstract":"Bercak darah dapat ditemukan di tempat kejadian perkara (TKP) pada banyak kasus tindak kekerasan. Asam deoksiribonukleat (DNA) pada darah dapat digunakan sebagai data primer untuk proses identifikasi akan tetapi bercak darah di TKP berisiko rusak akibat pajanan tanah dan ultraviolet. Tujuan utama dari penelitian ini adalah untuk mempelajari efek kombinasi dari pajanan sinar ultraviolet-C dan tanah terhadap kualitas dan kuantitas DNA pada bercak darah. Sebanyak 20 gelas berisi 200 gram tanah ditetesi 900µl darah dan diberikan pajanan sinar ultraviolet-C dalam tiga kelompok berdasarkan durasi pajanan yakni satu hari, tiga hari, dan lima hari. Satu kelompok digunakan sebagai kontrol. Ekstraksi DNA dilakukan menggunakan DNAZol dilanjutkan dengan pengukuran spektrofotometri untuk mengetahui kualitas dan kuantitas DNA. Peningkatan konsentrasi DNA dapat diamati yaitu 681,1 pada hari pertama menjadi 1274,7 pada hari ketiga dan mulai menurun menjadi 1090,6 pada hari kelima, sedangkan kemurnian DNA terus menurun secara konstan seiring dengan meningkatnya durasi pajanan. Penelitian ini menunjukkan bahwa pajanan sinar ultraviolet-C dan tanah menyebabkan degradasi molekul DNA menjadi fragmen-fragmen molekul yang lebih kecil sehingga terjadi peningkatan kuantitas DNA yang disertai penurunan kualitas DNA. Penurunan kualitas DNA dapat mempersulit proses identifikasi sehingga isolasi DNA sampel pada tanah terbuka yang terpajan matahari harus dilakukan sesegera mungkin. \u0000 \u0000Kata Kunci: DNA, darah, tanah, ultraviolet C, patologi forensik","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114571803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdominal obesity is a global public health problem. The high prevalence of abdominal obesity triggers an increase in morbidity and mortality in chronic diseases such as diabetes mellitus, cardiovascular, and cancer. One of the causes of the high prevalence of abdominal obesity is the lack of physical activity. Prevention efforts are needed through the identification of risk factors such as measurement of waist hip ratios. The respondents of this study were divided into two groups: 28 respondents who did zumba exercise for four weeks and 28 control respondents. The statistical test performed was a paired t-test comparison test. The results of this study indicate the value of t-count > t-table value (7.184 > 2.052) and the significance of 0.000 < 0.05. These results indicate that there is a decrease in the waist hip ratio after doing zumba exercise for four weeks.
{"title":"The Effect of Zumba Exercise to Women's Waist Hip Ratio","authors":"Laksmi Indreswari, Yunita Anggraeni, Rena Normasari","doi":"10.19184/AMS.V6I2.17449","DOIUrl":"https://doi.org/10.19184/AMS.V6I2.17449","url":null,"abstract":"Abdominal obesity is a global public health problem. The high prevalence of abdominal obesity triggers an increase in morbidity and mortality in chronic diseases such as diabetes mellitus, cardiovascular, and cancer. One of the causes of the high prevalence of abdominal obesity is the lack of physical activity. Prevention efforts are needed through the identification of risk factors such as measurement of waist hip ratios. The respondents of this study were divided into two groups: 28 respondents who did zumba exercise for four weeks and 28 control respondents. The statistical test performed was a paired t-test comparison test. The results of this study indicate the value of t-count > t-table value (7.184 > 2.052) and the significance of 0.000 < 0.05. These results indicate that there is a decrease in the waist hip ratio after doing zumba exercise for four weeks.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"499 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131768829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Indi Kamilia Fitri, Cholis Abrori, D. K. Dharmawan
In people with diabetes mellitus (DM) there is a decrease in basal vitamin C levels which is thought to be a result of oxidative stress in the condition of hyperglycemia that it needs to increase vitamin C as an antioxidant. Hyperglycemia in DM needs to be lowered by pharmacological therapy, named glimepirid so the purpose of this study is to determine the effectiveness of vitamin C addition to the reduction of KGD hyperglycemic mice with glimepirid treatment. This study is true experimental with a sample of 25 mice 20-30 grams and divided into five groups, first the control group (K0); STZ induction group (K1); STZ induction group with glimepirid treatment (K2); STZ induction group with the treatment of vitamin C (K3); STZ induction group by treatment of a combination of glimepirid and vitamin C (K4). STZ is injected intraperitonially 150 mg / kgBB. All groups were measured for KGD 1 after induction of STZ and KGD 2 after treatment for fourteen days. The results of the STZ induction group with the treatment of a combination of glimepirid and vitamin C (K4) experienced a smaller and not significant decrease in BSL in the STZ induction group with a single glimepirid (K2) treatment. These results are thought to have an influence from interactions between drugs that cause one drug to not work optimally. The conclusion of this study is that administration of glimepirid, vitamin C, or both can reduce blood glucose levels in hyperglycemic mice with the greatest decrease occurring in the glimepirid group.
{"title":"The Effectiveness of Vitamin C Addition to Blood Glucose Levels of Hyperglycemic Mice with Glimepiride Treatment","authors":"Indi Kamilia Fitri, Cholis Abrori, D. K. Dharmawan","doi":"10.19184/AMS.V6I3.9994","DOIUrl":"https://doi.org/10.19184/AMS.V6I3.9994","url":null,"abstract":"In people with diabetes mellitus (DM) there is a decrease in basal vitamin C levels which is thought to be a result of oxidative stress in the condition of hyperglycemia that it needs to increase vitamin C as an antioxidant. Hyperglycemia in DM needs to be lowered by pharmacological therapy, named glimepirid so the purpose of this study is to determine the effectiveness of vitamin C addition to the reduction of KGD hyperglycemic mice with glimepirid treatment. This study is true experimental with a sample of 25 mice 20-30 grams and divided into five groups, first the control group (K0); STZ induction group (K1); STZ induction group with glimepirid treatment (K2); STZ induction group with the treatment of vitamin C (K3); STZ induction group by treatment of a combination of glimepirid and vitamin C (K4). STZ is injected intraperitonially 150 mg / kgBB. All groups were measured for KGD 1 after induction of STZ and KGD 2 after treatment for fourteen days. The results of the STZ induction group with the treatment of a combination of glimepirid and vitamin C (K4) experienced a smaller and not significant decrease in BSL in the STZ induction group with a single glimepirid (K2) treatment. These results are thought to have an influence from interactions between drugs that cause one drug to not work optimally. The conclusion of this study is that administration of glimepirid, vitamin C, or both can reduce blood glucose levels in hyperglycemic mice with the greatest decrease occurring in the glimepirid group.","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132137168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thatit Nurmawati, Yeni Kartika Sari, Aprilia Putri Hidayat
HIV is a health problem for Indonesia and the world. Enforcement of diagnoses and control efforts continue to be made to reduce the number of HIV / AIDS cases. ARVs are becoming efforts to cure HIV by increasing CD4 cell counts. CD4 cell count is a determinant of ARV therapy. The purpose of this study was to evaluate CD4 cell counts in people with HIV / AIDS who were implementing ARV programs. The study design was observational using secondary data in the form of medical record records. The study population was HIV sufferers who registered to run an HIV / AIDS antiretrovial treatment program at the Cendana Poly Ngudi Waluyo Regional Hospital in Blitar in June 2017. Samples in the study were 30 people according to inclusion and exclusion criteria with a sampling technique using a purposive sampling method. Research instruments using questionnaires. Data analysis uses descriptive method. The results showed that this CD4 cell count had a CD4 cell count of 200 cells / mm 3 by 10%, 200-499 by 90% in patients who were on ARV programs. The patient's condition and obata resistance result in a CD4 cell count not exceeding 500 mm / cell. Key words: amount CD4, ARV , patient HIV
艾滋病毒是印度尼西亚和全世界的一个健康问题。继续执行诊断和控制工作,以减少艾滋病毒/艾滋病病例的数量。抗逆转录病毒药物正成为通过增加CD4细胞计数来治愈艾滋病毒的努力。CD4细胞计数是抗逆转录病毒治疗的决定因素。本研究的目的是评估正在实施抗逆转录病毒治疗方案的艾滋病毒/艾滋病患者的CD4细胞计数。本研究采用观察性设计,采用医疗记录形式的辅助资料。研究人群是艾滋病毒患者,他们于2017年6月在布利塔的Cendana Poly Ngudi Waluyo地区医院注册了艾滋病毒/艾滋病抗逆转录病毒治疗项目。本研究采用有目的的抽样方法,按纳入和排除标准取样30人。使用问卷调查的研究工具。数据分析采用描述性方法。结果表明,在接受抗逆转录病毒治疗的患者中,CD4细胞计数为200个细胞/ mm 3的比例为10%,200-499的比例为90%。患者的病情和抗氧性导致CD4细胞计数不超过500mm /细胞。关键词:CD4, ARV, HIV患者
{"title":"Evaluation of CD4 Number In HIV / AIDS Patients Who Are Running The Antiretrovial Treatment Program","authors":"Thatit Nurmawati, Yeni Kartika Sari, Aprilia Putri Hidayat","doi":"10.19184/AMS.V6I2.16790","DOIUrl":"https://doi.org/10.19184/AMS.V6I2.16790","url":null,"abstract":"HIV is a health problem for Indonesia and the world. Enforcement of diagnoses and control efforts continue to be made to reduce the number of HIV / AIDS cases. ARVs are becoming efforts to cure HIV by increasing CD4 cell counts. CD4 cell count is a determinant of ARV therapy. The purpose of this study was to evaluate CD4 cell counts in people with HIV / AIDS who were implementing ARV programs. The study design was observational using secondary data in the form of medical record records. The study population was HIV sufferers who registered to run an HIV / AIDS antiretrovial treatment program at the Cendana Poly Ngudi Waluyo Regional Hospital in Blitar in June 2017. Samples in the study were 30 people according to inclusion and exclusion criteria with a sampling technique using a purposive sampling method. Research instruments using questionnaires. Data analysis uses descriptive method. The results showed that this CD4 cell count had a CD4 cell count of 200 cells / mm 3 by 10%, 200-499 by 90% in patients who were on ARV programs. The patient's condition and obata resistance result in a CD4 cell count not exceeding 500 mm / cell. \u0000Key words: amount CD4, ARV , patient HIV","PeriodicalId":246242,"journal":{"name":"Journal of Agromedicine and Medical Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115316941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}