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Cryopreservation of in vitro-produced bovine embryos by vitrification: In pursuit of a simplified, standardized procedure that improves pregnancy rates to promote cattle industry use 通过玻璃化冷冻保存体外产生的牛胚胎:追求一种简化、标准化的程序,以提高怀孕率,促进养牛业的使用
Pub Date : 1900-01-01 DOI: 10.2298/bah2003251h
Van Do Huong, W. A. Taylor-Robinson
The goal of cryopreservation is to retain the original stage of gametes and embryos after they have endured cooling and warming. Slow freezing is a standard method for in vivo-derived bovine embryo cryopreservation, threefifths of such embryos being frozen by this method globally. However, it is evident that slow freezing is not efficient for cryopreserving in vitro-produced bovine embryos. Hence, only one-third of in vitro-produced bovine embryos are cryopreserved. Vitrification is a preferred method for storage of human embryos; consequently, it has been explored as a novel means to store in vitro-produced bovine embryos, for which it shows considerable promise as an alternative to slow freezing. This is due to several reasons: vitrification is often less time-consuming than slow freezing; it does not need expensive slow rate freezing machines; and it has been proven to have comparatively higher survival rates. Yet, in the cattle industry vitrification continues to present shortcomings, such as possible toxicity of vitrification solutions and failure to standardize methods, which pose a challenge for its application to in vitro-produced bovine embryos. Therefore, determining the most suitable procedure is crucial to make vitrification more practical in commercial settings.
低温保存的目的是在配子和胚胎经历冷却和升温后保持其原始状态。慢速冷冻是体外源性牛胚胎冷冻保存的标准方法,全球五分之三的胚胎采用慢速冷冻方法冷冻。然而,很明显,慢速冷冻对体外产生的牛胚胎的冷冻保存效率不高。因此,只有三分之一的体外生产的牛胚胎被冷冻保存。玻璃化是保存人类胚胎的首选方法;因此,它已被探索作为一种新的手段来储存体外生产的牛胚胎,为此,它显示出相当大的希望,作为一种替代缓慢冷冻。这是由于几个原因:玻璃化通常比缓慢冷冻更节省时间;它不需要昂贵的慢速冷冻机;并且已经被证明有相对较高的存活率。然而,在养牛业中,玻璃化仍然存在缺点,例如玻璃化溶液的可能毒性和标准化方法的失败,这对其在体外生产的牛胚胎中的应用构成了挑战。因此,确定最合适的程序对于使玻璃化在商业环境中更加实用至关重要。
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引用次数: 2
Influence of some factors on fertility and weight if sheep and body weight development of lambs 某些因素对绵羊生育能力、体重及羔羊体重发育的影响
Pub Date : 1900-01-01 DOI: 10.2298/bah2202093l
Nemanja Lečić, V. Caro-Petrovic, D. Ružić-Muslić, Nevena Maksimović, Bogdan Cekić, I. Ćosić
The research included a total of 921 lambs, 474 ewes and 8 sjenicke sheep rams from 4 different farms. The aim of the research was to examine the influence of the farm, cultivation technology and the influence of rams within the farm on production indicators. Statistical analysis showed that the body weight of lambs at birth ranged from 3.37 to 4.03 kg (average 3.68 kg), at 30 days from 11.51 to 12.41 kg (average 12.07 kg) and from 90 days from 27.82 to 29 kg (average 28.65 kg). A statistically highly significant influence of the farm was determined (P<0.01) on the body weight of lambs in all three control periods, as well as on the body weight of sheep and on the fertility of sheep. The influence of the ram on the body weight of the lambs at birth was statistically very significant within the farm (P<0.01). When it comes to the percentage share of birth type by farm, farm 1 had the most singletons (59.13%) both within the farm and in comparison between other farms, while there were fewer twins (40.87%), and triplets were not identified. On the other farms, the percentage of twins was the highest, and triplets were also present, while on farm 4 there were also quadruplets, lambs born as quadruplets (3.28%).
该研究包括来自4个不同农场的921只羔羊、474只母羊和8只舍尼克公羊。研究的目的是考察农场、养殖技术和农场内公羊对生产指标的影响。统计分析表明,羔羊出生时体重为3.37 ~ 4.03 kg(平均3.68 kg), 30日龄体重为11.51 ~ 12.41 kg(平均12.07 kg), 90日龄体重为27.82 ~ 29 kg(平均28.65 kg)。猪场对3个对照期羔羊体重、绵羊体重和绵羊生育能力的影响均具有极显著的统计学意义(P<0.01)。公羊对羔羊出生时体重的影响在场内极显著(P<0.01)。就农场出生类型的百分比而言,农场1在农场内和与其他农场相比,单胎最多(59.13%),而双胞胎较少(40.87%),三胞胎未被确定。在其他农场,双胞胎的比例最高,也有三胞胎,而在4号农场也有四胞胎,即四胞胎出生的羔羊(3.28%)。
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引用次数: 0
Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” fish farm, Serbia 来自塞尔维亚“Braduljica”渔场的褐鳟(Salmo trutta)遗传结构的特征
Pub Date : 1900-01-01 DOI: 10.2298/bah1903289m
Nikola Molerović, B. Rašković, R. Djedović, Ostojic Andric, Z. Markovic, S. Marić
Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock.
出于生态保护和遗传资源保护的考虑,在“Braduljica”养鱼场对褐鳟(Salmo trutta)进行了遗传结构表征。从10个个体的鳍夹中分离DNA,利用分子PCR-RFLP方法,根据线粒体DNA控制区(CR mtDNA)和核DNA乳酸脱氢酶基因(LDH nDNA)进行大西洋和多瑙河血统的区分。根据表型特征估计,本研究中包括的10个个体中有5个属于异种大西洋谱系,其余5个属于本地多瑙河褐鳟谱系。然而,分子分析结果显示,个体中有很高比例的异源基因,这证实了这两个谱系之间的杂交。结果还表明,基于表型的选择是不充分的。为了继续进行适当的亲鱼管理,有必要从亲鱼中消除大西洋血统的异体个体。
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引用次数: 1
The effect of space allowance in the cage and floor systems on feather condition and egg production 笼底系统空间余量对羽毛状况和产蛋量的影响
Pub Date : 1900-01-01 DOI: 10.2298/bah2202125s
Z. Škrbić, M. Lukić, V. Petričević, S. Bogosavljević-Bošković, S. Rakonjac, V. Dosković, N. Tolimir
The egg production sector is in a transitional period with regard to the permitted housing systems, i.e. rearing in conventional cages under certain conditions to the complete ban of any form of cage system. The changes were caused by concern for the layer wellfare but with the expected effects on productivity as well. The aim of the research was to determine the effects of the floor space allowance in cage and non-cage housing systems on the feather score and egg production of laying hens of three ages, from the aspect of the regulated minimum and optimal space in the cage system (C) and the space provided in the extensive rearing system with hens in smaller groups in the facility (extensive indoor -EI). In order to determine the feather score, the body weight of the laying hens and the weight of the eggs, three groups of laying hens were formed: C4 (564 cm2 per hen, cage system), C3 (751 cm2 per hen, cage system) and EI ( 3000 cm2 per hen, extensive indoor). The feather score and body weight of laying hens were determined in three ages of hens (30, 40, 50 weeks), by individual assessment and measurement of all hens in the experiment. The hen-day egg production and egg weight were determined in the same weeks of laying age. In addition to the expected decrease in feather score with the age of hens, results indicated a significant interaction between age and space allowance per hen. Observed by individual body parts, as well as based on the overall feather score, the space allowance per hen exhibited a full, cumulative effect at 50 weeks of age. Based on the space allowance, it was possible to rank the overall feather score, with the laying hens with the most space having the best feather score. The effect of the space allowance on the body weight of the laying hens was manifested through the space available on the feeder, which resulted in the lowest (p<0.01) body weight values recorded in laying hens of the C4 group. Egg production was not significantly influenced by the space allowance per hen (p=0.069), but a connection between egg production and the housing system can be concluded. The average egg weight, in addition to the known effect of layer age, was the lowest (p<0.01) in the group with the least space allowance per hen.
蛋品生产部门正处于从允许的饲养系统,即在某些条件下在传统笼中饲养到完全禁止任何形式的笼系统的过渡时期。这些变化是出于对阶层福利的关注,但也有对生产力的预期影响。本研究的目的是从笼养系统(C)中调节的最小和最佳空间以及设施中较小群体母鸡的粗放式饲养系统(广泛的室内-EI)中提供的空间两方面来确定笼养和非笼养系统的占地面积对三个年龄的蛋鸡羽毛评分和产蛋量的影响。为测定蛋鸡的羽毛评分、体重和蛋重,将蛋鸡分为3组:C4组(564 cm2 /只,笼养系统)、C3组(751 cm2 /只,笼养系统)和EI组(3000 cm2 /只,广泛室内)。在30、40、50周龄,通过对所有试验母鸡进行个体评估和测量,测定蛋鸡的羽毛评分和体重。在同一产蛋周龄测定母鸡日产蛋量和蛋重。除了羽毛评分随母鸡年龄的增加而下降外,结果还表明年龄和每只母鸡的空间允许量之间存在显著的相互作用。从个体身体部位和整体羽毛评分来看,每只母鸡的空间裕度在50周龄时表现出充分的累积效应。基于空间允许,可以对整体羽毛评分进行排序,空间最大的蛋鸡羽毛评分最好。空间裕度对蛋鸡体重的影响主要体现在饲粮上的可用空间,C4组蛋鸡的体重最低(p<0.01)。每只母鸡的空间裕度对产蛋量影响不显著(p=0.069),但产蛋量与鸡舍系统有关。除已知的产蛋日龄影响外,平均蛋重在每只母鸡预留空间最小的组最低(p<0.01)。
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引用次数: 0
Morphometric characterization of Pirot pramenka 梨果的形态计量学特征
Pub Date : 1900-01-01 DOI: 10.2298/bah2102075r
D. Ružić-Muslić, M. Petrović, Bogdan Cekić, I. Ćosić, I. Pavlović, Nevena Maksimović, V. Caro-Petrovic
The strategy of conservation of endangered sheep populations implies morphological and genetic characterization, as basic preconditions for their conservation. The aim of this study was to determine the morphometric characteristics, their correlations and the index of physical development of Pirot pramenka, which has the status of the most endangered population in Serbia. The measuring was performed on 30 sheep, aged 3 years, reared in the area of Stara Planina. The descriptive statistical procedure was performed using the statistical package Statistica (version 8). The average height at the withers was 56.31 cm, body length 62.93 cm, chest width 18.37 cm, chest depth 25.96 cm, chest circumference 77.59 cm, shin circumference 6.70 cm. The strongest and significantly positive correlation (P <0.05) was found between chest depth and height at withers (0.65), body length (0.58) and body weight (0.56). Pirotska pramenka has slightly higher indices of format, chest and massiveness, in relation to breeds Vitoroga Zuja and Travnik Pramenka. These morphometric traits suggest that, compared to previous research, the body frame of Pirot pramenka sheep did not change significantly, which means that there were no crosses with other breeds due to geographical isolation and enthusiasm of breeders to preserve the indigenous Pirot pramenka which was the starting point for following authentic brands: Pirot lamb, cheese and carpet. Hence the biological and moral imperative: to preserve this highly endangered population.
濒危绵羊种群的保护策略包括形态和遗传特征,这是保护濒危绵羊种群的基本前提。本研究的目的是确定塞尔维亚最濒危种群Pirot pramenka的形态特征,它们之间的相关性和身体发育指数。该测量是在Stara Planina地区饲养的30只3岁绵羊上进行的。描述性统计程序采用统计软件包Statistica (version 8)进行。马肩峰平均身高56.31 cm,体长62.93 cm,胸宽18.37 cm,胸深25.96 cm,胸围77.59 cm,胫围6.70 cm。胸深与马肩高(0.65)、体长(0.58)、体重(0.56)呈显著正相关(P <0.05)。与品种Vitoroga Zuja和Travnik pramenka相比,Pirotska pramenka的外形、胸部和体重指数略高。这些形态特征表明,与之前的研究相比,Pirot pramenka羊的身体结构没有明显变化,这意味着由于地理隔离和育种者对保护本土Pirot pramenka的热情,没有与其他品种杂交,这是以下正宗品牌的起点:Pirot羔羊,奶酪和地毯。因此,保护这一高度濒危的种群是生物学和道德上的当务之急。
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引用次数: 0
Associative effects of a mixed Brachiaria decumbens and Pennisetum purpureum grass feed on the ensiling properties 卧卧臂草与紫尾狼尾草混合饲料对青贮性能的联合效应
Pub Date : 1900-01-01 DOI: 10.2298/bah2301103m
Alban Mugoti, N. Chikumba, Anderson Munengwa, Lenin Dziwanyika, Sizo Moyo, Chiedza Mgumba
This study aimed to evaluate the effects of the association between two tropical grasses, Signal grass (Brachiaria decumbens) and Napier grass (Pennisetum purpureum) on the proximate composition, in vitro digestibility, and fermentation characteristics of ensiled material. A complete randomized design was used on three treatments namely Signal grass, Napier grass, and Signal-Napier grass combination. Silage was made using molasses applied in proportions of 1:2 with water and mixed with silage at the rate of 5% for a 5kg bag of 2.5cm cut grass. Signal-Napier grass combination silage was superior in almost all parameters to the two sole crop silages. After ensiling, a significant difference (p<0.05) in most parameters was recorded. A significant difference (p=0.0004) in pH was determined where pH was lower in the sole Napier grass silage than that of the Signal-Napier combination and Signal grass. A similar outcome (P<0.05) was recorded for crude protein, ash, ether extracts, nitrogen-free extracts, and neutral detergent fiber. However, no significant difference (p>0.05) was obtained in dry matter (p=0.1524), crude fiber (p=0.5924), and ADF (p=0.1168). Although having poor digestibility values in all treatments, Signal grass proved to be better than the rest. Organoleptic characteristics were promising, with normal color, smell, and texture changes observed. These results indicated that the association of grasses had an impressive positive effect on the nutritional value and quality of silages. Therefore, the use of mixed grass silages is encouraged.
本研究旨在评价信号草(Brachiaria decumbens)和纳匹尔草(Pennisetum purpureum)两种热带牧草的杂交对青贮材料的近似组成、体外消化率和发酵特性的影响。采用完全随机设计,分别对信号草、纳皮尔草和信号-纳皮尔草组合3个处理进行试验。青贮采用糖蜜与水的比例为1:2,以5%的比例混合青贮,每袋5kg割下2.5cm的草。信号-纳匹尔草组合青贮几乎在所有参数上都优于两种单一作物青贮。青贮后干物质(p=0.1524)、粗纤维(p=0.5924)和ADF (p=0.1168)差异显著(p0.05)。虽然所有处理的消化率都较差,但信号草的消化率优于其他处理。感官特征是有希望的,观察到正常的颜色,气味和质地变化。上述结果表明,牧草的配种对青贮饲料的营养价值和品质有显著的积极影响。因此,建议使用混合草料青贮。
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引用次数: 0
Effects of light intensity in different stocking densities on tibial measurements and incidence of lesions in broilers 不同饲养密度下光照强度对肉鸡胫骨尺寸及病变发生率的影响
Pub Date : 1900-01-01 DOI: 10.2298/bah1903243s
Z. Škrbić, M. Lukić, V. Petričević, S. Bogosavljević-Bošković, S. Rakonjac, V. Dosković, N. Tolimir
The possibility for use of high light intensity in broiler production management to improve the health of broilers? legs was examined in a study aiming to determine the effect of two levels of light intensity in different stocking densities of broiler chickens. The study was carried out on 1200 Ross 308 broilers according to a random block system with 6 treatments (2 x 3 factorial design) and 4 replicates per treatment. The examined light intensity levels of 150 lx (high light intensity-HLI) and 20 lx (low light intensity-LLI) were applied to 3 stocking densities of broilers: 10, 13 and 15 birds/m2. Scoring of foot pad (FP) and hock burns (HB) was carried out on the 42nd day, on a sample of 240 broilers. Osteometric and biomechanical measurements on tibia were performed after slaughtering and primary processing, on a sample of 72 broilers. The high level intensity of light exerted no significant effect on the development and firmness of the tibia of broilers grown in the densities of 10, 13 and 15 birds/m2. There are indications that in the highest studied stocking density the increased light intensity can improve the individual properties of tibia. The differences in the average assessment of hock burns were concluded to be the result of the stocking density that was observed only in conditions of the increased intensity of light. The average score of foot pad lesions was under the significant main effect of both investigated factors. The differences in the average FP estimation between the light intensity treatments were confirmed in the stocking density of 13 birds/m2.
在肉鸡生产管理中利用高光强改善肉鸡健康的可能性?在一项旨在确定两种光照强度对不同饲养密度肉鸡的影响的研究中,对腿进行了检查。试验选用1200只罗斯308肉鸡,采用随机区组设计,6个处理(2 × 3因子设计),每个处理4个重复。试验采用150 lx(强光强- hli)和20 lx(弱光强- lli)光照水平,分别对3个饲养密度:10、13和15只/m2的肉鸡进行光照。试验第42天对240只肉鸡进行足垫(FP)和飞节烧伤(HB)评分。在屠宰和初级加工后,对72只肉鸡样本进行胫骨骨测量和生物力学测量。高强度光照对生长密度为10、13和15只/m2的肉仔鸡胫骨发育和硬度无显著影响。有迹象表明,在研究的最高放养密度下,增加光强可以改善胫骨的个体性能。在飞节烧伤的平均评估上的差异被认为是放养密度的结果,而放养密度只在光照强度增加的条件下观察到。足底病变的平均评分均受两种因素的显著主作用。在13只/m2的放养密度下,不同光强处理的平均FP估计值存在差异。
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引用次数: 1
Farmers’ economic interest in Dermanyssus gallinae control 鸡皮蚜防治对农民经济利益的影响
Pub Date : 1900-01-01 DOI: 10.2298/bah2103171p
А. Pavlicevic, I. Pavlović, S. Kulić
Poultry red mite or Dermanyssus gallinae (De Geer, 1778) is the most significant poultry ectoparasite with regards to health and economy. It is a widely accepted opinion that D. gallinae can only be suppressed, with the current annual expenditure of 60 eurocents per layer. However, research indicates that D. gallinae can be controlled in other ways and eradicated from the production facilities and farms, and subsequent reinfestation can be prevented by implementing biosafety measures. This provides a long-term or permanent effect of D. gallinae control. From the aspect of economy, this means that after decades of increasing expenditures, farmers can first decrease, and then completely eliminate expenditures incurred by D. gallinae. Therefore, economic calculations should be based on an expert and comprehensive approach, which should itself be based on rational control, preventive veterinary medicine, i.e. D. gallinae control program. This would result in long-term savings. In 10 years? time, 0.5 million euros would be saved per 100.000 layers. There are an estimated 4 billion infested layers worldwide.
家禽红螨或Dermanyssus gallinae (De Geer, 1778)是最重要的家禽外寄生虫。人们普遍认为鸡鸡D. gallinae只能被抑制,目前每年每层花费60欧分。然而,研究表明,鸡肠弧菌可以通过其他方式控制,从生产设施和农场中根除,并通过实施生物安全措施防止随后的再感染。这提供了一个长期或永久的效果,鸡D.控制。从经济角度来看,这意味着农民在经过几十年的支出增加后,可以先减少,然后完全消除鸡戎杆菌的支出。因此,经济计算应建立在专家综合方法的基础上,而专家综合方法本身应建立在合理控制、预防兽药即鸡蛉控制方案的基础上。这将带来长期的节省。10年后?每10万层可节省50万欧元。据估计,全世界有40亿层细菌被感染。
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引用次数: 1
Some quantitative genetic traits in Vietnamese indigenous Noi chicken from 0 to 28 days old 0 ~ 28日龄越南乡土Noi鸡的一些数量遗传性状
Pub Date : 1900-01-01 DOI: 10.2298/bah1902141k
Do Khoa Vo, N. Thi, Nguyen. Thao, N. Thi, S. Okamoto, K. Kawabe, Takeshi Shimogigri
The aim of this study is to characterize some quantitative traits of Noi chicken, one of the Vietnamese famous native breeds for meat quality and fighting, at the stage of 0-28 days old. Therefore, 742 chicks were used to measure, record, analyze and evaluate on these traits. As results, there was significant difference in observation traits among stages of age (P=0.000). Interesting, the wings (+64.88%) and keel lengths (+58.83%) rapidly developed within the first week of life, followed by the most of other dimensions, especially breast diameter (+31.85%), thigh diameter (+71.17%) in the second week of age. An increase in the size of the measurements was due to development of the skeleton at observing time points along the experiment. However, there was strong development of muscle tissue in the second week of life as rate of the breast and thigh diameter was greatest. Noi chicks consumed amount of feed of 16.54 g/bird/day and gained a weight of 6.98 g/bird/day. Thus, their feed conversion ratio was 2.37 at the stage of 0-28 days old. Perhaps a direct positive relationship between observed traits and age were randomly established according biological characteristics of animal. This work provided initial benchmarks of Noi chicks for further studies.
本研究旨在对越南肉质和肉质优良的著名地方品种Noi鸡0 ~ 28日龄阶段的一些数量性状进行研究。因此,选用742只雏鸡对这些性状进行测量、记录、分析和评价。结果表明,各年龄阶段观察性状差异有统计学意义(P=0.000)。有趣的是,翅膀(+64.88%)和龙骨长度(+58.83%)在出生后第一周内发育迅速,其次是其他尺寸,尤其是胸径(+31.85%)和大腿直径(+71.17%)在第二周龄发育迅速。测量尺寸的增加是由于沿着实验的观察时间点骨骼的发展。然而,在出生后的第二周,肌肉组织发育强劲,乳房和大腿直径的增长速度最大。饲粮用量为16.54 g/只/d,增重为6.98 g/只/d。0 ~ 28日龄饲料系数为2.37。也许观察到的性状与年龄之间的直接正相关关系是根据动物的生物学特性随机建立起来的。这项工作为今后的研究提供了初步的基准。
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引用次数: 4
Biodiversity of ticks of sheep and goats in semi-intensive farming system in Vojvodina 伏伊伏丁那省半集约化农业系统中绵羊和山羊蜱虫的生物多样性
Pub Date : 1900-01-01 DOI: 10.2298/bah1903267p
I. Pavlović, S. Ivanović, M. Petrović, V. Caro-Petrovic, Z. Becskei, M. Savić, Ferenc Csordás
The present study was conducted in 90 sheep and goats flocks from the territory of Vojvodina in the two years period of March 2016 to October 2017, during the grazing season. Tick infestation was deteected in 53.14% of examined animals. The most dominant was Ixodes ricinus (43.91%), followed by Dermacentor marginatus (31.91%), Rhipicephalus bursa (15.22%), R.sanguineus (8.72%), Hyalomma savignyi (3.72%),, Haemaphysalis punctata (3.21%) and D.pictus (2.72%) . The sex ratio of detected tick species showed a higher number of females in four species (I.ricinus, H.punctata, R.sanguineus and D.marginatus), while higher number of males were detected in two species (R.bursa and Hy.savignyi), and an equal number of ticks of the D.pictus. The population dynamics of recorded tick species showed two annual maxima, in spring (April- May) and in autumn (September-October). The considerable interchange between spring and autumn tick populations can be attributed mainly to environmental conditions.
本研究在2016年3月至2017年10月的放牧季节对伏伊伏丁那省境内的90只绵羊和山羊进行了研究。53.14%的被检动物检出蜱虫。其次是边缘革蜱(31.91%)、法氏鼻头蜱(15.22%)、血蜱(8.72%)、沙氏透明眼蜱(3.72%)、斑点血蜱(3.21%)和画蚊(2.72%)。检测到的蜱种性别比显示,4种蜱(蓖麻蜱、马点蜱、血蜱和边缘蜱)雌性数量较多,2种蜱(法氏蜱和萨维伊蜱)雄性数量较多,图片蜱数量相等。蜱类种群动态有春季(4 ~ 5月)和秋季(9 ~ 10月)两个高峰。春季和秋季蜱虫种群之间的大量交换主要归因于环境条件。
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引用次数: 0
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Biotehnologija u stocarstvu
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