The aim of this work was a phenotypic description of the mare families of a Lipizzan horse breed from stud Vucijak. A total of 31 mares were measured, for every animal 28 measures were recorded. The mares are distributed by mare families in the following: Sana (3), Lipa (3), Bregava (3), Cremica (3), Ukrina (3), Visla (2), Neretva (3), Pliva (1), Drina (2), Sutjeska (2), Sitnica (1), Janja (2) and Sava. Simple analysis of variance was done to determine the difference in the morphological measures between mare families. Also, correlation between 28 measures was done. This study revealed phenotypic uniformity between mare families. Of the 28 measures recorded, a statistically significant difference was identified only for the length of cannon (front leg). The longest average length of the cannon (front leg) had at Sana (24.67 cm), and the shortest at Bregava (19.00 cm). The correlation ranged from slightly negative to highly positive with correlation coefficients from -0.465 to 0.779. Significant and highly significant correlation with height, length and depth was found in mare measures. The body shape of the mare families has a rectangular, which is in accordance with the Lipizzan horses from other stud in Europe. Also, the study revealed that mares from Vucijak are smaller than mares from other stud, which is in accordance with the breeding goals.
{"title":"Estimation of phenotypic variability of body measurements in Lipizzan mares","authors":"Biljana Rogic, B. Važić, Ivica Ravić","doi":"10.2298/bah1904399r","DOIUrl":"https://doi.org/10.2298/bah1904399r","url":null,"abstract":"The aim of this work was a phenotypic description of the mare families of a\u0000 Lipizzan horse breed from stud Vucijak. A total of 31 mares were measured,\u0000 for every animal 28 measures were recorded. The mares are distributed by\u0000 mare families in the following: Sana (3), Lipa (3), Bregava (3), Cremica\u0000 (3), Ukrina (3), Visla (2), Neretva (3), Pliva (1), Drina (2), Sutjeska (2),\u0000 Sitnica (1), Janja (2) and Sava. Simple analysis of variance was done to\u0000 determine the difference in the morphological measures between mare\u0000 families. Also, correlation between 28 measures was done. This study\u0000 revealed phenotypic uniformity between mare families. Of the 28 measures\u0000 recorded, a statistically significant difference was identified only for the\u0000 length of cannon (front leg). The longest average length of the cannon\u0000 (front leg) had at Sana (24.67 cm), and the shortest at Bregava (19.00 cm).\u0000 The correlation ranged from slightly negative to highly positive with\u0000 correlation coefficients from -0.465 to 0.779. Significant and highly\u0000 significant correlation with height, length and depth was found in mare\u0000 measures. The body shape of the mare families has a rectangular, which is in\u0000 accordance with the Lipizzan horses from other stud in Europe. Also, the\u0000 study revealed that mares from Vucijak are smaller than mares from other\u0000 stud, which is in accordance with the breeding goals.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133202985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalila Bengouga, L. LahmadiSalwa, Reguia Zeguerou, M. Maaoui, Y. Halis
A livestock survey conducted during 2013/2015 as part of a CRSTRA project in 4 villages situated at North east Biskra and south Batna in Algeria. These regions are located at elevation ranging around 250-831m asl, experiencing arid and semi-arid Mediterranean climate. Respondents of 86 families demonstrated that livestock is an integral part of the region?s mixed farming systems. Low livestock numbers per most households at present reflect the self-consumption breeding mode adapted in these regions. Currently, farmers focus on four main livestock types; goat, sheep, chicken and bee keeping in two regions, it is the case of Beni Souik and Branis , while Maafa includes beyond these types, turkey and pigeon whereas Ain Zaatout includes duck and swine beside the previous livestock types. In the same context; goat ranked first in the four regions, goat and sheep secondly then goat and poultry with goat combined to sheep and poultry in third place. Thus; most families use a combination of grazing, agriculture sub-products and industrial products for the nutrition of their livestock. Families keep livestock as source of milk, butter, wool or hair, leather and other products that are strongly used as nutritional, weaving supply or stocking covering resources for the family members or friends and in some cases for sell to seekers of animal products of indigenous territory origins. Most families use these products for family and friend consumption while a minority sell some of them on local markets.
{"title":"Livestock in rural piedmont regions of Algeria","authors":"Khalila Bengouga, L. LahmadiSalwa, Reguia Zeguerou, M. Maaoui, Y. Halis","doi":"10.2298/bah1902199b","DOIUrl":"https://doi.org/10.2298/bah1902199b","url":null,"abstract":"A livestock survey conducted during 2013/2015 as part of a CRSTRA project in\u0000 4 villages situated at North east Biskra and south Batna in Algeria. These\u0000 regions are located at elevation ranging around 250-831m asl, experiencing\u0000 arid and semi-arid Mediterranean climate. Respondents of 86 families\u0000 demonstrated that livestock is an integral part of the region?s mixed\u0000 farming systems. Low livestock numbers per most households at present\u0000 reflect the self-consumption breeding mode adapted in these regions.\u0000 Currently, farmers focus on four main livestock types; goat, sheep, chicken\u0000 and bee keeping in two regions, it is the case of Beni Souik and Branis ,\u0000 while Maafa includes beyond these types, turkey and pigeon whereas Ain\u0000 Zaatout includes duck and swine beside the previous livestock types. In the\u0000 same context; goat ranked first in the four regions, goat and sheep secondly\u0000 then goat and poultry with goat combined to sheep and poultry in third\u0000 place. Thus; most families use a combination of grazing, agriculture\u0000 sub-products and industrial products for the nutrition of their livestock.\u0000 Families keep livestock as source of milk, butter, wool or hair, leather and\u0000 other products that are strongly used as nutritional, weaving supply or\u0000 stocking covering resources for the family members or friends and in some\u0000 cases for sell to seekers of animal products of indigenous territory\u0000 origins. Most families use these products for family and friend consumption\u0000 while a minority sell some of them on local markets.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122069469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Zivkovic, B. Stanković, Č. Radović, M. Gogić, A. Stanojković, S. Obradović, N. Stojiljković
The effects of the use of antibiotics and garlic powder in the nutrition of weaning piglets were compared. The trial included 120 weaned piglets of the same genotype (Large White) distributed in three feeding treatments. In the first study period (day 27-56), a feed mixture with 20% protein was used, while in the second period (day 57-84), a mixture with 18% protein. The control group was fed with mixtures containing antibiotics in the amount of 0.2%, while the two experimental groups were fed with mixtures containing fermented garlic powder (FGP) in the concentration of 0.05% and 0.2%. The obtained results showed that the use of FGP, instead of antibiotics, resulted in better gain in second trial group, and also better feed conversion ratio, in both trial groups by 3.84% and 6.93% respectively, during the entire research period.
{"title":"Garlic as alternative for antibiotics in diet for growing pigs","authors":"V. Zivkovic, B. Stanković, Č. Radović, M. Gogić, A. Stanojković, S. Obradović, N. Stojiljković","doi":"10.2298/bah1903281z","DOIUrl":"https://doi.org/10.2298/bah1903281z","url":null,"abstract":"The effects of the use of antibiotics and garlic powder in the nutrition of\u0000 weaning piglets were compared. The trial included 120 weaned piglets of the\u0000 same genotype (Large White) distributed in three feeding treatments. In the\u0000 first study period (day 27-56), a feed mixture with 20% protein was used,\u0000 while in the second period (day 57-84), a mixture with 18% protein. The\u0000 control group was fed with mixtures containing antibiotics in the amount of\u0000 0.2%, while the two experimental groups were fed with mixtures containing\u0000 fermented garlic powder (FGP) in the concentration of 0.05% and 0.2%. The\u0000 obtained results showed that the use of FGP, instead of antibiotics,\u0000 resulted in better gain in second trial group, and also better feed\u0000 conversion ratio, in both trial groups by 3.84% and 6.93% respectively,\u0000 during the entire research period.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128492093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Fatai, M. Akinyemi, O. Osaiyuwu, K. Ewuola, A. Salako
Slow growth rate and acute heat stress are among the major constraints to indigenous chicken production in Nigeria. Characterization of heat-tolerance genes is a requisite for selective breeding of poultry for improved heat-tolerance and productivity. Therefore, this study investigated variation in HSP70 gene and its association with heat-tolerance traits of Nigerian locally adapted chickens. One-day old chicks comprising 118 Yoruba Ecotype Chicken-YEC and 138 FUNAAlpha Chicken-FAC were tagged and fed ad-libitum on starter (0-6 weeks) and grower (7-24 weeks) diets. At week 12, blood was sampled, DNA was extracted, amplified and electrophoresed following standard procedures. HSP70 gene was genotyped using MmeI restriction endonuclease. At week 23, 36 chickens (six chickens per identified HSP70 genotypes) selected from each of YEC and FAC were exposed to 40?1?C for one hour. Cloaca temperature, respiratory rate (RR) and pulse rate (PR) were recorded and heat stress index (HSI) calculated. Data were analysed using descriptive statistics and ANOVA. Alleles A and B with genotypes: AA, AB, and BB were detected. After acute heat-stress, YEC with BB had higher RR value compared to those with AA and AB. The PR value of FAC with genotype BB was significantly higher (p<0.05) than those of AA, but similar to chickens with AB. Within FAC, HSI of BB-HSP70 was lower than AA-HSP70 but similar to AB-HSP70, while within YEC HSI of BB-HSP70 was similar to those of AA-HSP70 and AB-HSP70. The HSP70 gene was polymorphic in the studied chickens and genotype BB-HSP70 was associated with thermo-tolerance.
{"title":"Physiological response of Nigerian locally adapted chickens with different heat shock protein 70 genotypes to acute heat stress","authors":"R. Fatai, M. Akinyemi, O. Osaiyuwu, K. Ewuola, A. Salako","doi":"10.2298/bah2301051f","DOIUrl":"https://doi.org/10.2298/bah2301051f","url":null,"abstract":"Slow growth rate and acute heat stress are among the major constraints to\u0000 indigenous chicken production in Nigeria. Characterization of heat-tolerance\u0000 genes is a requisite for selective breeding of poultry for improved\u0000 heat-tolerance and productivity. Therefore, this study investigated\u0000 variation in HSP70 gene and its association with heat-tolerance traits of\u0000 Nigerian locally adapted chickens. One-day old chicks comprising 118 Yoruba\u0000 Ecotype Chicken-YEC and 138 FUNAAlpha Chicken-FAC were tagged and fed\u0000 ad-libitum on starter (0-6 weeks) and grower (7-24 weeks) diets. At week\u0000 12, blood was sampled, DNA was extracted, amplified and electrophoresed\u0000 following standard procedures. HSP70 gene was genotyped using MmeI\u0000 restriction endonuclease. At week 23, 36 chickens (six chickens per\u0000 identified HSP70 genotypes) selected from each of YEC and FAC were exposed\u0000 to 40?1?C for one hour. Cloaca temperature, respiratory rate (RR) and pulse\u0000 rate (PR) were recorded and heat stress index (HSI) calculated. Data were\u0000 analysed using descriptive statistics and ANOVA. Alleles A and B with\u0000 genotypes: AA, AB, and BB were detected. After acute heat-stress, YEC with\u0000 BB had higher RR value compared to those with AA and AB. The PR value of FAC\u0000 with genotype BB was significantly higher (p<0.05) than those of AA, but\u0000 similar to chickens with AB. Within FAC, HSI of BB-HSP70 was lower than\u0000 AA-HSP70 but similar to AB-HSP70, while within YEC HSI of BB-HSP70 was\u0000 similar to those of AA-HSP70 and AB-HSP70. The HSP70 gene was polymorphic in\u0000 the studied chickens and genotype BB-HSP70 was associated with\u0000 thermo-tolerance.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127146668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Djedović, V. Bogdanović, D. Stanojević, H. Ismael, D. Janković, S. Trivunović, L. Samolovac, T. Stamenic
Data set including 10860 primiparous Holstein-Friesian breed cows first calved in the period from 2011 to 2015, was used in determining phenotypic variability and correlation between the traits of milk yield and linearly estimated traits of udder and angularity. The average values obtained for type traits (angularity, fore udder attachment, front teats placement, teats length, udder depth and rear udder height) were 6.47; 5.74; 4.96; 5.20; 5.99 and 6.25, respectively. The values obtained for phenotypic correlations between linear type traits and traits of milk yield ranged from -0.042 (udder depth and milk yield) to 0.335 (fore udder attachment and protein yield). Positive phenotypic correlation (0.293) was recorded also between fore udder attachment and milk yield which is deemed the most important trait of milk yield while the lowest correlation between milk yields was determined in relation to udder depth (-0.033). The results obtained indicate a possibility of applying direct and indirect multiple traits selection which should be conducted within a national progeny-testing programme on Holstein-Friesian bulls by using the method of selection indexes.
{"title":"Phenotypic characteristics of linear traits of udder and angularity in Holstein-Friesian cows and their correlation with milk yield traits","authors":"R. Djedović, V. Bogdanović, D. Stanojević, H. Ismael, D. Janković, S. Trivunović, L. Samolovac, T. Stamenic","doi":"10.2298/BAH2004407D","DOIUrl":"https://doi.org/10.2298/BAH2004407D","url":null,"abstract":"Data set including 10860 primiparous Holstein-Friesian breed cows first\u0000 calved in the period from 2011 to 2015, was used in determining phenotypic\u0000 variability and correlation between the traits of milk yield and linearly\u0000 estimated traits of udder and angularity. The average values obtained for\u0000 type traits (angularity, fore udder attachment, front teats placement, teats\u0000 length, udder depth and rear udder height) were 6.47; 5.74; 4.96; 5.20; 5.99\u0000 and 6.25, respectively. The values obtained for phenotypic correlations\u0000 between linear type traits and traits of milk yield ranged from -0.042\u0000 (udder depth and milk yield) to 0.335 (fore udder attachment and protein\u0000 yield). Positive phenotypic correlation (0.293) was recorded also between\u0000 fore udder attachment and milk yield which is deemed the most important\u0000 trait of milk yield while the lowest correlation between milk yields was\u0000 determined in relation to udder depth (-0.033). The results obtained\u0000 indicate a possibility of applying direct and indirect multiple traits\u0000 selection which should be conducted within a national progeny-testing\u0000 programme on Holstein-Friesian bulls by using the method of selection\u0000 indexes.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130047897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Despite raising animals mostly as scavengers in the guinea savannah zone of Ghana, there is still scanty scientific information on the effects of this system on the health of these animals. A study was conducted to document factors influencing the prevalence rate of gastrointestinal (GIT) parasites and their loads in 500 small ruminants (250 each of sheep and goats). Prevalence rate of about 86% was recorded in small ruminants. Sheep, however, had higher (P<0.05) overall GIT parasite burden than goats. In sheep, significantly (P<0.05) more females harboured strongyles than males. Similarly, tapeworm and GIT parasites in general, were more prevalent (P<0.05) in younger than older sheep. Strongyle population was higher (P<0.05) in growers than adults, while Eimeria spp populations were higher (P<0.05) in lambs and adults than growers. The overall GIT parasite load, however, was higher (P<0.05) in lambs than all other age groups. In goats, Eimeria spp infections, coinfections of Strongyloides spp/Eimeria spp, tapeworm/Emeria spp, and overall GIT parasite load were much higher (P<0.05) in the extensive than the semi-intensive systems of management. The overall parasite population was also higher (P<0.05) in growers than adults and kids. Coinfections of tapeworm/ Strongyloides spp increased (P<0.05) with increasing age. The prevalence rate of GIT parasites in small ruminants is high. However, higher GIT parasite burden was found in sheep than goats. Younger small ruminants and goats kept in the extensive system of management are more prone to GIT parasite infestation than those kept in the semi-intensive system and older ones, respectively.
{"title":"Gastrointestinal parasite infections in small ruminants relative to host sex, age and husbandry system under the guinea savannah vegetation","authors":"I. Abdul-Rahman, Paintsil Fuachie, Makija Tati","doi":"10.2298/bah2202139a","DOIUrl":"https://doi.org/10.2298/bah2202139a","url":null,"abstract":"Despite raising animals mostly as scavengers in the guinea savannah zone of\u0000 Ghana, there is still scanty scientific information on the effects of this\u0000 system on the health of these animals. A study was conducted to document\u0000 factors influencing the prevalence rate of gastrointestinal (GIT) parasites\u0000 and their loads in 500 small ruminants (250 each of sheep and goats).\u0000 Prevalence rate of about 86% was recorded in small ruminants. Sheep,\u0000 however, had higher (P<0.05) overall GIT parasite burden than goats. In\u0000 sheep, significantly (P<0.05) more females harboured strongyles than males.\u0000 Similarly, tapeworm and GIT parasites in general, were more prevalent\u0000 (P<0.05) in younger than older sheep. Strongyle population was higher\u0000 (P<0.05) in growers than adults, while Eimeria spp populations were higher\u0000 (P<0.05) in lambs and adults than growers. The overall GIT parasite load,\u0000 however, was higher (P<0.05) in lambs than all other age groups. In goats,\u0000 Eimeria spp infections, coinfections of Strongyloides spp/Eimeria spp,\u0000 tapeworm/Emeria spp, and overall GIT parasite load were much higher (P<0.05)\u0000 in the extensive than the semi-intensive systems of management. The overall\u0000 parasite population was also higher (P<0.05) in growers than adults and\u0000 kids. Coinfections of tapeworm/ Strongyloides spp increased (P<0.05) with\u0000 increasing age. The prevalence rate of GIT parasites in small ruminants is\u0000 high. However, higher GIT parasite burden was found in sheep than goats.\u0000 Younger small ruminants and goats kept in the extensive system of management\u0000 are more prone to GIT parasite infestation than those kept in the\u0000 semi-intensive system and older ones, respectively.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130591358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ibifiri Briggs Prekuna, M. Nchang, W. Mathew, Ikechukwu James Joseph
KiSS-1 gene encodes a protein product kisspeptin which are intense inducers of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion in various mammalian species through its receptor GPR54 (G protein-coupled receptor-54). A total of 100 goat compromising of Red Sokoto (n = 72) and Sahel (n = 28) breeds were used to detect single nucleotide polymorphisms (SNPs) in the intronic region of the KiSS-1 gene by sequencing and investigate their relationship with other goat breeds. Nucleotide sequence analysis revealed five novel SNPs (g.1745G>A present in Red Sokoto, g.1776G>A, g.1827A>G, g.1857T>C and g.2208T>C present in Red Sokoto and Sahel breeds). To obtain a correct phylogenetic relationship between goat breeds, nucleotide sequences were compared to other sequences in NCBI database using a BLASTn algorithm and retrieved for further analysis. Neighbour-joining phylogenetic relationship tree constructed revealed two distinct clusters with ancestral lineage of 100% identity. Nigerian goat breeds (Red Sokoto and Sahel) clustered into a clade with Indian goat breeds (Ganjam and Osmanabadi) while the second cluster involved eight other goat breeds. Genetic distance estimate revealed high genetic similarity between Red Sokoto and Sahel breeds as observed in their genetic distance value of 0.003. The nucleotide sequences of the two Nigerian goat breeds (Red Sokoto and Sahel) for KiSS-1 gene were submitted to GenBank database and have accession numbers: MN122316 and MN122317, respectively. The analysis of polymorphism in KiSS-1 gene indicates that genetic variation exists in the goat breeds studied. Therefore, attempts can be made to investigate the association of these polymorphism with reproductive traits in Nigerian goat breeds.
KiSS-1基因编码的蛋白产物kisspeptin通过其受体GPR54 (G蛋白偶联受体-54)在多种哺乳动物体内强烈诱导黄体生成素(LH)和促卵泡激素(FSH)的分泌。利用红索科托山羊(72)和萨赫勒山羊(28)共100只山羊，通过测序检测了KiSS-1基因内子区域的单核苷酸多态性(snp)，并研究了它们与其他山羊品种的关系。核苷酸序列分析发现5个新snp (G . 1745g >A, G . 1776g >A, G . 1827a >G, G . 1857t >C和G . 2208t >C)。为了获得山羊品种间正确的系统发育关系，利用BLASTn算法将核苷酸序列与NCBI数据库中的其他序列进行比较，并检索核苷酸序列进行进一步分析。构建的亲缘亲缘关系树显示出两个不同的群体，其祖先谱系同源性为100%。尼日利亚山羊品种(Red Sokoto和Sahel)与印度山羊品种(Ganjam和Osmanabadi)聚集在一个分支中，而第二个集群涉及其他八个山羊品种。遗传距离估计显示，红索科托和萨赫勒品种之间的遗传相似性很高，遗传距离值为0.003。将2个尼日利亚山羊品种(Red Sokoto和Sahel)的KiSS-1基因核苷酸序列提交到GenBank数据库，其登录号分别为MN122316和MN122317。KiSS-1基因多态性分析表明，所研究的山羊品种存在遗传变异。因此，可以尝试研究这些多态性与尼日利亚山羊品种繁殖性状的关系。
{"title":"Sequencing, polymorphism and phylogenetic characterisation of KiSS-1 gene in two Nigerian indigenous goat breeds","authors":"Ibifiri Briggs Prekuna, M. Nchang, W. Mathew, Ikechukwu James Joseph","doi":"10.2298/bah2003283b","DOIUrl":"https://doi.org/10.2298/bah2003283b","url":null,"abstract":"KiSS-1 gene encodes a protein product kisspeptin which are intense inducers\u0000 of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion\u0000 in various mammalian species through its receptor GPR54 (G protein-coupled\u0000 receptor-54). A total of 100 goat compromising of Red Sokoto (n = 72) and\u0000 Sahel (n = 28) breeds were used to detect single nucleotide polymorphisms\u0000 (SNPs) in the intronic region of the KiSS-1 gene by sequencing and\u0000 investigate their relationship with other goat breeds. Nucleotide sequence\u0000 analysis revealed five novel SNPs (g.1745G>A present in Red Sokoto,\u0000 g.1776G>A, g.1827A>G, g.1857T>C and g.2208T>C present in Red Sokoto and\u0000 Sahel breeds). To obtain a correct phylogenetic relationship between goat\u0000 breeds, nucleotide sequences were compared to other sequences in NCBI\u0000 database using a BLASTn algorithm and retrieved for further analysis.\u0000 Neighbour-joining phylogenetic relationship tree constructed revealed two\u0000 distinct clusters with ancestral lineage of 100% identity. Nigerian goat\u0000 breeds (Red Sokoto and Sahel) clustered into a clade with Indian goat breeds\u0000 (Ganjam and Osmanabadi) while the second cluster involved eight other goat\u0000 breeds. Genetic distance estimate revealed high genetic similarity between\u0000 Red Sokoto and Sahel breeds as observed in their genetic distance value of\u0000 0.003. The nucleotide sequences of the two Nigerian goat breeds (Red Sokoto\u0000 and Sahel) for KiSS-1 gene were submitted to GenBank database and have\u0000 accession numbers: MN122316 and MN122317, respectively. The analysis of\u0000 polymorphism in KiSS-1 gene indicates that genetic variation exists in the\u0000 goat breeds studied. Therefore, attempts can be made to investigate the\u0000 association of these polymorphism with reproductive traits in Nigerian goat\u0000 breeds.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"329 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114196014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Do Khoa Vo, N. Thi, N. Thi, S. Okamoto, Kataro Kawabe, Nguyen Khang Thi, Nguyen Giang Tuyet, Takeshi Shimogigri
Noi chicken is one of the most popular native breeds in the South of Vietnam because their good meat quality and disease resistance. Therefore, although price of their products (eggs and meat) is always high, they are always preferred. However, there have not been many studies on the morphology of this breed yet. In the current study, a total of 355 Noi chickens (164 males and 191 females) at 28 days old were selected and randomly allotted to individual cages until they reached the market weight at 84 days old. During the experiment, Noi broilers were fed ad libitum a diet with 17% crude protein and 3.000 kcal/kg ME produced by GreenFeed Vietnam Joint Stock Company. Average daily gain, feed conversion ratio and some morphological dimensions were recorded at two different stages of age (28-56 and 56-84 days old). Results were demonstrated that (i) significant differences in feed intake (P=0.006), body weight (P=0.019), beak length (P=0.014), thigh length (P=0.004), shank length (P=0.003), breast diameter (P=0.000), and thigh diameter (P=0.001) were found between males and females as well as between different stages; and (ii) body weight and some dimensions will increase rapidly at the first stage. An understanding on these economic traits helps researchers and producers have a more detailed glance on developing of each part of Noi chicken at different stages of age which they are the basic scientific foundation for further studies on this breed.
{"title":"Growth performance and morphology of in 28-84 day-old Vietnamese local Noi chicken","authors":"Do Khoa Vo, N. Thi, N. Thi, S. Okamoto, Kataro Kawabe, Nguyen Khang Thi, Nguyen Giang Tuyet, Takeshi Shimogigri","doi":"10.2298/bah1903301k","DOIUrl":"https://doi.org/10.2298/bah1903301k","url":null,"abstract":"Noi chicken is one of the most popular native breeds in the South of Vietnam\u0000 because their good meat quality and disease resistance. Therefore, although\u0000 price of their products (eggs and meat) is always high, they are always\u0000 preferred. However, there have not been many studies on the morphology of\u0000 this breed yet. In the current study, a total of 355 Noi chickens (164 males\u0000 and 191 females) at 28 days old were selected and randomly allotted to\u0000 individual cages until they reached the market weight at 84 days old. During\u0000 the experiment, Noi broilers were fed ad libitum a diet with 17% crude\u0000 protein and 3.000 kcal/kg ME produced by GreenFeed Vietnam Joint Stock\u0000 Company. Average daily gain, feed conversion ratio and some morphological\u0000 dimensions were recorded at two different stages of age (28-56 and 56-84\u0000 days old). Results were demonstrated that (i) significant differences in\u0000 feed intake (P=0.006), body weight (P=0.019), beak length (P=0.014), thigh\u0000 length (P=0.004), shank length (P=0.003), breast diameter (P=0.000), and\u0000 thigh diameter (P=0.001) were found between males and females as well as\u0000 between different stages; and (ii) body weight and some dimensions will\u0000 increase rapidly at the first stage. An understanding on these economic\u0000 traits helps researchers and producers have a more detailed glance on\u0000 developing of each part of Noi chicken at different stages of age which they\u0000 are the basic scientific foundation for further studies on this breed.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114244082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haruna Abimiku Kaki, Comfort Tuleun Dooshima, Oluwabiyi Oluremi Ikeolu, E. F. Akumbugu
A feeding trial was conducted with two hundred (200) Arbor Acre strain of broiler chicks to determine the effect of soybean residue (SBR) on carcass characteristics and visceral organs. Birds were fed for 28 days (starter phase) with formulated diet containing 22% crude protein and 3000Kcal/kg (ME). Five iso-nitrogenous experimental diets were formulated which contain SBR. In the control diet (T1) groundnut cake was served as the only protein source. Four (4) test diets designated as: T2, T3, T4 and T5 were formulated with SBR replacing 25, 50, 75 and 100% of groundnut cake respectively in finisher phase. The birds were randomly grouped into five (5) experimental treatment groups in four (4) replicates of 40 birds per treatment in a completely randomized design. The finisher phase lasted for five (5) weeks and the birds were fed and given drinking water adlibitum. All carcass characteristics evaluated differ significantly (P<0.05) among treatment groups except breast and wings. No significant (P>0.05) difference were shown for organs, while shanks was only the residue that was significantly affected by dietary treatment. Soybean residue can be used up to 100% to replace groundnut cake in the diet of broiler chickens thus providing a productive use for this hither to neglected agro allied waste.
{"title":"Effect of replacement of soybean residues for groundnut cake on carcass yield of broiler chickens","authors":"Haruna Abimiku Kaki, Comfort Tuleun Dooshima, Oluwabiyi Oluremi Ikeolu, E. F. Akumbugu","doi":"10.2298/bah2003317a","DOIUrl":"https://doi.org/10.2298/bah2003317a","url":null,"abstract":"A feeding trial was conducted with two hundred (200) Arbor Acre strain of\u0000 broiler chicks to determine the effect of soybean residue (SBR) on carcass\u0000 characteristics and visceral organs. Birds were fed for 28 days (starter\u0000 phase) with formulated diet containing 22% crude protein and 3000Kcal/kg\u0000 (ME). Five iso-nitrogenous experimental diets were formulated which contain\u0000 SBR. In the control diet (T1) groundnut cake was served as the only protein\u0000 source. Four (4) test diets designated as: T2, T3, T4 and T5 were formulated\u0000 with SBR replacing 25, 50, 75 and 100% of groundnut cake respectively in\u0000 finisher phase. The birds were randomly grouped into five (5) experimental\u0000 treatment groups in four (4) replicates of 40 birds per treatment in a\u0000 completely randomized design. The finisher phase lasted for five (5) weeks\u0000 and the birds were fed and given drinking water adlibitum. All carcass\u0000 characteristics evaluated differ significantly (P<0.05) among treatment\u0000 groups except breast and wings. No significant (P>0.05) difference were\u0000 shown for organs, while shanks was only the residue that was significantly\u0000 affected by dietary treatment. Soybean residue can be used up to 100% to\u0000 replace groundnut cake in the diet of broiler chickens thus providing a\u0000 productive use for this hither to neglected agro allied waste.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125271454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Škrbić, M. Lukić, V. Petričević, S. Bogosavljević-Bošković, S. Rakonjac, V. Dosković, N. Tolimir
The comparison of physical quality properties of table eggs of commercial hybrid Tetra SL and two native indigenous breeds of hens, Banat Naked Neck and Svrljig hen, was performed in conditions of alternative production in the pasture system in portable cages without the floor. The experiment was conducted during the vegetation period, at the age of hens of 53-57 weeks. In order to produce good quality eggs, the diet was designed with a complete mixture based on maize and soybean. In addition, constant availability of pastures was ensured. Egg sampling was performed in the morning and the initial egg quality was examined based on egg weight, shape index, egg white, egg yolk and shell quality parameters. Layer hens of the commercial hybrid Tetra SL had significantly higher egg weight and better internal egg quality (p<0.01) compared to hens of Banat Naked Neck and Svrljig hens, except for the colour of the yolk, which was more intense in the same feeding and breeding conditions and the age of laying hens, in eggs of native breeds (p<0.01). Native breeds in relation to the commercial hybrid had lighter shell colour (p<0.01). Deformation and egg shell thickness differed (p<0.01) between all three genotypes. The determined rank of layer hen genotypes for shell thickness was: Tetra SL, Banat Naked Neck, Svrljig hen. However, the breaking force was without statistically confirmed difference between hen genotypes.
{"title":"Quality of eggs from pasture rearing layers of different genotypes","authors":"Z. Škrbić, M. Lukić, V. Petričević, S. Bogosavljević-Bošković, S. Rakonjac, V. Dosković, N. Tolimir","doi":"10.2298/bah2002181s","DOIUrl":"https://doi.org/10.2298/bah2002181s","url":null,"abstract":"The comparison of physical quality properties of table eggs of commercial\u0000 hybrid Tetra SL and two native indigenous breeds of hens, Banat Naked Neck\u0000 and Svrljig hen, was performed in conditions of alternative production in\u0000 the pasture system in portable cages without the floor. The experiment was\u0000 conducted during the vegetation period, at the age of hens of 53-57 weeks.\u0000 In order to produce good quality eggs, the diet was designed with a complete\u0000 mixture based on maize and soybean. In addition, constant availability of\u0000 pastures was ensured. Egg sampling was performed in the morning and the\u0000 initial egg quality was examined based on egg weight, shape index, egg\u0000 white, egg yolk and shell quality parameters. Layer hens of the commercial\u0000 hybrid Tetra SL had significantly higher egg weight and better internal egg\u0000 quality (p<0.01) compared to hens of Banat Naked Neck and Svrljig hens,\u0000 except for the colour of the yolk, which was more intense in the same\u0000 feeding and breeding conditions and the age of laying hens, in eggs of\u0000 native breeds (p<0.01). Native breeds in relation to the commercial hybrid\u0000 had lighter shell colour (p<0.01). Deformation and egg shell thickness\u0000 differed (p<0.01) between all three genotypes. The determined rank of layer\u0000 hen genotypes for shell thickness was: Tetra SL, Banat Naked Neck, Svrljig\u0000 hen. However, the breaking force was without statistically confirmed\u0000 difference between hen genotypes.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131644094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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