Pub Date : 2025-02-28Epub Date: 2025-01-30DOI: 10.1021/acssensors.4c03118
Sohyun Park, Kihyun Kim, Anna Go, Min-Ho Lee, Lingxin Chen, Jaebum Choo
Bacterial infections, such as sepsis, require prompt and precise identification of the causative bacteria for appropriate antibiotics treatment. Traditional methods such as culturing take 2-5 days, while newer techniques such as reverse transcription-polymerase chain reaction and mass spectrometry are hindered by blood impurities. Consequently, this study developed a surface-enhanced Raman scattering (SERS)-based acoustofluidic technique for rapid bacterial detection without culturing or lysing. Target bacteria are first tagged with SERS nanotags in a microtube. The solution with tagged bacteria and unbound SERS nanotags is passed through a silicon microfluidic channel. A piezoelectric transducer generates acoustic waves within the channel, concentrating larger tagged bacteria in the center and pushing smaller unbound nanotags toward the channel walls. A laser beam is focused at the center of the channel, and the Raman signals of bacteria passing through the focal volume are measured for quantitative analysis. As a proof of concept, this study detected various concentrations of Escherichia coli at a limit of detection of 1.75 × 105 CFU/mL within 1 h. This method offers significant clinical potential, enabling rapid and accurate bacterial identification without genetic material extraction, cultivation, or lysis.
{"title":"Rapid and Sensitive <i>Escherichia coli</i> Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform.","authors":"Sohyun Park, Kihyun Kim, Anna Go, Min-Ho Lee, Lingxin Chen, Jaebum Choo","doi":"10.1021/acssensors.4c03118","DOIUrl":"10.1021/acssensors.4c03118","url":null,"abstract":"<p><p>Bacterial infections, such as sepsis, require prompt and precise identification of the causative bacteria for appropriate antibiotics treatment. Traditional methods such as culturing take 2-5 days, while newer techniques such as reverse transcription-polymerase chain reaction and mass spectrometry are hindered by blood impurities. Consequently, this study developed a surface-enhanced Raman scattering (SERS)-based acoustofluidic technique for rapid bacterial detection without culturing or lysing. Target bacteria are first tagged with SERS nanotags in a microtube. The solution with tagged bacteria and unbound SERS nanotags is passed through a silicon microfluidic channel. A piezoelectric transducer generates acoustic waves within the channel, concentrating larger tagged bacteria in the center and pushing smaller unbound nanotags toward the channel walls. A laser beam is focused at the center of the channel, and the Raman signals of bacteria passing through the focal volume are measured for quantitative analysis. As a proof of concept, this study detected various concentrations of <i>Escherichia coli</i> at a limit of detection of 1.75 × 10<sup>5</sup> CFU/mL within 1 h. This method offers significant clinical potential, enabling rapid and accurate bacterial identification without genetic material extraction, cultivation, or lysis.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"1217-1227"},"PeriodicalIF":8.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-28Epub Date: 2025-01-31DOI: 10.1021/acssensors.4c03073
Yaxing Zhang, Zhiwei Cai, Rong Zou, Ruling Wang, Runan Tan, Lei Wang, Yuxiang Wu, Hanping He, Yunbin He, Gang Chang
Epithelial cell adhesion molecule (EpCAM) was considered to be an important marker of multiple tumors, and its high expression is closely related to the early diagnosis and treatment of tumors. At present, metal oxide semiconductors have become a key component of biosensor and bioelectronics technology. Tin oxide shows great potential for development because of its nontoxic, nonpolluting, low price, and excellent electrical properties. In this study, a novel SnO2 solution-gated thin film transistor (SGTFT) biosensor for the specific detection of EpCAM was successfully developed using SnO2 film prepared by the sol-gel method as the channel material. By selecting the optimal thickness of 100 nm SnO2 film as the channel material, the transconductance value (gm) reached 1432 μS, and the threshold voltage (Vth) remained stable at 0.288 V. In order to achieve qualitative and quantitative detection of EpCAM, SnO2 films were subjected to a specific chemical treatment to fix the aptamer. Through a specific recognition between the aptamer and EpCAM, the gate voltage changes were triggered to regulate the channel current of the device. FE-SEM, EIS, XPS, and electrical performance tests were employed to track and measure the modification process. Based on the optimizations described above, the prepared SGTFT exhibited high detection sensitivity (14.6 mV·dec-1), the limit of detection (LOD) down to 24.4 pg/mL, and the calibration curves in the range of 0.02 ng/mL-500 ng/mL for EpCAM sensing. The developed SnO2-SGTFT biosensor is anticipated to provide a new highly sensitive and specific detection platform for health monitoring and disease diagnosis.
{"title":"Solution-Gated Thin Film Transistor Biosensor-Based SnO<sub>2</sub> Amorphous Film for Label-Free Detection of Epithelial Cell Adhesion Molecules.","authors":"Yaxing Zhang, Zhiwei Cai, Rong Zou, Ruling Wang, Runan Tan, Lei Wang, Yuxiang Wu, Hanping He, Yunbin He, Gang Chang","doi":"10.1021/acssensors.4c03073","DOIUrl":"10.1021/acssensors.4c03073","url":null,"abstract":"<p><p>Epithelial cell adhesion molecule (EpCAM) was considered to be an important marker of multiple tumors, and its high expression is closely related to the early diagnosis and treatment of tumors. At present, metal oxide semiconductors have become a key component of biosensor and bioelectronics technology. Tin oxide shows great potential for development because of its nontoxic, nonpolluting, low price, and excellent electrical properties. In this study, a novel SnO<sub>2</sub> solution-gated thin film transistor (SGTFT) biosensor for the specific detection of EpCAM was successfully developed using SnO<sub>2</sub> film prepared by the sol-gel method as the channel material. By selecting the optimal thickness of 100 nm SnO<sub>2</sub> film as the channel material, the transconductance value (<i>g</i><sub>m</sub>) reached 1432 μS, and the threshold voltage (<i>V</i><sub>th</sub>) remained stable at 0.288 V. In order to achieve qualitative and quantitative detection of EpCAM, SnO<sub>2</sub> films were subjected to a specific chemical treatment to fix the aptamer. Through a specific recognition between the aptamer and EpCAM, the gate voltage changes were triggered to regulate the channel current of the device. FE-SEM, EIS, XPS, and electrical performance tests were employed to track and measure the modification process. Based on the optimizations described above, the prepared SGTFT exhibited high detection sensitivity (14.6 mV·dec<sup>-1</sup>), the limit of detection (LOD) down to 24.4 pg/mL, and the calibration curves in the range of 0.02 ng/mL-500 ng/mL for EpCAM sensing. The developed SnO<sub>2</sub>-SGTFT biosensor is anticipated to provide a new highly sensitive and specific detection platform for health monitoring and disease diagnosis.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"1187-1196"},"PeriodicalIF":8.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-27DOI: 10.1021/acssensors.4c03003
Seong-Geun Jeong, Youjin Lee, Hye-Seon Jeong, Seong Jun Park, Jinki Yeom, Chang-Hyung Choi, Byung-Gee Kim
Probiotic metabolites are gaining attention as potential antibiotic candidates against antibiotic-resistant bacteria. The disk diffusion test, by measuring bacterial aggregate responses, faces challenges in accurately evaluating antimicrobial efficacy when these responses to different probiotic strains are indistinguishable at a macroscopic level. Here, this study presents an analytical method for accurately evaluating antimicrobial activity by analyzing bacterial cell proliferation suppression at a microscopic level. This assay can be used in a coculture system, designed to continuously expose pathogenic bacteria growing on the bottom surface of the culture plate to probiotic metabolites, selectively released from porous capsules positioned above. Bacterial proliferation is optically monitored in real-time and tracked via a computer vision algorithm. Specifically, bacterial proliferation is quantified as their doubling time, calculated using a proposed stochastic kinetic model. This method identifies the most potent antimicrobial strains by determining which probiotic candidates most effectively extend the bacterial doubling time. In comparative experiments using the same strains, this proposed method demonstrated clear distinctions in the antimicrobial efficacy of each strain, unlike the disk diffusion test. Therefore, this approach provides a reliable solution for identifying superior probiotic strains, with potential for widespread use in discovering new antimicrobial agents.
{"title":"Probiotic Antimicrobial Evaluation Via Real-Time Profiling of Bacterial Cell Proliferation Using Stochastic Kinetics","authors":"Seong-Geun Jeong, Youjin Lee, Hye-Seon Jeong, Seong Jun Park, Jinki Yeom, Chang-Hyung Choi, Byung-Gee Kim","doi":"10.1021/acssensors.4c03003","DOIUrl":"https://doi.org/10.1021/acssensors.4c03003","url":null,"abstract":"Probiotic metabolites are gaining attention as potential antibiotic candidates against antibiotic-resistant bacteria. The disk diffusion test, by measuring bacterial aggregate responses, faces challenges in accurately evaluating antimicrobial efficacy when these responses to different probiotic strains are indistinguishable at a macroscopic level. Here, this study presents an analytical method for accurately evaluating antimicrobial activity by analyzing bacterial cell proliferation suppression at a microscopic level. This assay can be used in a coculture system, designed to continuously expose pathogenic bacteria growing on the bottom surface of the culture plate to probiotic metabolites, selectively released from porous capsules positioned above. Bacterial proliferation is optically monitored in real-time and tracked via a computer vision algorithm. Specifically, bacterial proliferation is quantified as their doubling time, calculated using a proposed stochastic kinetic model. This method identifies the most potent antimicrobial strains by determining which probiotic candidates most effectively extend the bacterial doubling time. In comparative experiments using the same strains, this proposed method demonstrated clear distinctions in the antimicrobial efficacy of each strain, unlike the disk diffusion test. Therefore, this approach provides a reliable solution for identifying superior probiotic strains, with potential for widespread use in discovering new antimicrobial agents.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"51 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the realm of intelligent sensing, the development of multifunctional, highly sensitive, wide-ranging, and durable flexible sensors remains a formidable challenge. This paper introduces a dual-mode ionic gel sensor, inspired by the sensory mechanisms of ants and developed through magnetically induced technology capable of simultaneously detecting environmental humidity and pressure. The humidity sensor is fabricated from a prestretched, buckled poly(vinyl alcohol) (PVA) ionic gel with microstructures, exhibiting rapid response and high durability. The voltage signal of the humidity sensor decreases linearly with increasing relative humidity (RH). The pressure sensor employs a capacitive structure, integrating magnetically induced fiber pilose structures with ionic gel, achieving a high sensitivity of 7.375 kPa–1 and an excellent linear response over a broad pressure range from 0.5 Pa to 95 kPa. In the context of human motion monitoring, the sensor accurately captures physiological signals, such as pulse beats and joint movements while concurrently detecting skin humidity. Additionally, by leveraging deep learning algorithms, the sensor attains a remarkable 99.21% accuracy in object recognition within flexible intelligent sorting systems, underscoring its potential in smart logistics sorting applications. This study transcends the traditional limitations of capacitive pressure sensors regarding sensitivity and detection range, offering novel solutions for applications in motion monitoring and intelligent logistics sorting.
在智能传感领域，开发多功能、高灵敏度、大范围和耐用的柔性传感器仍然是一项艰巨的挑战。本文介绍了一种双模离子凝胶传感器，其灵感来源于蚂蚁的感觉机制，并通过磁感应技术开发而成，能够同时检测环境湿度和压力。该湿度传感器是由具有微结构的预拉伸、扣压聚乙烯醇（PVA）离子凝胶制成的，具有响应速度快、耐用性高等特点。湿度传感器的电压信号随相对湿度（RH）的增加而线性下降。压力传感器采用电容式结构，将磁感应纤维柔毛结构与离子凝胶融为一体，灵敏度高达 7.375 kPa-1，并在 0.5 Pa 至 95 kPa 的宽压力范围内具有出色的线性响应。在人体运动监测方面，该传感器可准确捕捉脉搏跳动和关节运动等生理信号，同时检测皮肤湿度。此外，通过利用深度学习算法，该传感器在灵活的智能分拣系统中的物体识别准确率达到了惊人的 99.21%，彰显了其在智能物流分拣应用中的潜力。这项研究突破了电容式压力传感器在灵敏度和检测范围方面的传统限制，为运动监测和智能物流分拣应用提供了新颖的解决方案。
{"title":"Ant-Inspired Ion Gel Sensor for Dual-Mode Detection of Force and Humidity via Magnetic Induction","authors":"Boyi Xu, Jinsui Xu, Haoran Ou, Feihu Song, Zheping Wang, Ye Tian, Yifan Lu, Zhangqing Duan, Fei Yang, Yitong Zhou","doi":"10.1021/acssensors.5c00032","DOIUrl":"https://doi.org/10.1021/acssensors.5c00032","url":null,"abstract":"In the realm of intelligent sensing, the development of multifunctional, highly sensitive, wide-ranging, and durable flexible sensors remains a formidable challenge. This paper introduces a dual-mode ionic gel sensor, inspired by the sensory mechanisms of ants and developed through magnetically induced technology capable of simultaneously detecting environmental humidity and pressure. The humidity sensor is fabricated from a prestretched, buckled poly(vinyl alcohol) (PVA) ionic gel with microstructures, exhibiting rapid response and high durability. The voltage signal of the humidity sensor decreases linearly with increasing relative humidity (RH). The pressure sensor employs a capacitive structure, integrating magnetically induced fiber pilose structures with ionic gel, achieving a high sensitivity of 7.375 kPa<sup>–1</sup> and an excellent linear response over a broad pressure range from 0.5 Pa to 95 kPa. In the context of human motion monitoring, the sensor accurately captures physiological signals, such as pulse beats and joint movements while concurrently detecting skin humidity. Additionally, by leveraging deep learning algorithms, the sensor attains a remarkable 99.21% accuracy in object recognition within flexible intelligent sorting systems, underscoring its potential in smart logistics sorting applications. This study transcends the traditional limitations of capacitive pressure sensors regarding sensitivity and detection range, offering novel solutions for applications in motion monitoring and intelligent logistics sorting.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"27 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Flexible wearable potentiometric ion sensors for continuous monitoring of electrolyte cations have made significant advances in bioanalysis for personal healthcare and diagnostics. However, less attention is paid to the most abundant extracellular anion, chloride ion (Cl–) as a mark of electrolyte imbalance and an important diagnostic indicator of cystic fibrosis, which has important significance for accurate monitoring in complex biological fluids. An all-solid-state Cl–-selective electrode is constructed utilizing oxygen vacancies reinforced vanadium oxide with a nitrogen-doped carbon shield as the solid contact (V2O3–x@NC/Cl–-ISE). The prepared V2O3–x@NC/Cl–-ISE exhibits a low detection limit of 10–5.45 M without an interfacial water layer and shows a highly stable potential with 7.24 μV/h during 24 h, which is attributed to the rapid interfacial electron transfer of the conductive carbon layers and the valence state transition of the polyvalent vanadium center in charge storage processes. Additionally, the custom flexible sensing patch presents an excellent sensitivity retention rate under bending (95%) and twisting (93%) strains and possesses good anti-interference performance (ΔE < 8 mV) against common interfering ions and organic substances in sweat. Real-time monitoring of the Cl– concentration in sweat aligns with ion chromatography analysis results. This study presents a compact wearable Cl– monitoring platform for the easy tracking of exercise-induced dehydration and cystic fibrosis screening with promising applications in smart healthcare.
{"title":"Integrated Headband for Monitoring Chloride Anions in Sweat Using Developed Flexible Patches","authors":"Chen-Lu Wang, Xin Cai, Yong-Huan Zhao, Zi-Hao Liu, Rui-Ze Xia, Li-Jun Tang, Zong-Yin Song, Shi-Hua Chen, Yixiang Li, Meng Yang, Pei-Hua Li, Xing-Jiu Huang","doi":"10.1021/acssensors.4c03366","DOIUrl":"https://doi.org/10.1021/acssensors.4c03366","url":null,"abstract":"Flexible wearable potentiometric ion sensors for continuous monitoring of electrolyte cations have made significant advances in bioanalysis for personal healthcare and diagnostics. However, less attention is paid to the most abundant extracellular anion, chloride ion (Cl<sup>–</sup>) as a mark of electrolyte imbalance and an important diagnostic indicator of cystic fibrosis, which has important significance for accurate monitoring in complex biological fluids. An all-solid-state Cl<sup>–</sup>-selective electrode is constructed utilizing oxygen vacancies reinforced vanadium oxide with a nitrogen-doped carbon shield as the solid contact (V<sub>2</sub>O<sub>3</sub><sub>–<i>x</i></sub>@NC/Cl<sup>–</sup>-ISE). The prepared V<sub>2</sub>O<sub>3</sub><sub>–<i>x</i></sub>@NC/Cl<sup>–</sup>-ISE exhibits a low detection limit of 10<sup>–5.45</sup> M without an interfacial water layer and shows a highly stable potential with 7.24 μV/h during 24 h, which is attributed to the rapid interfacial electron transfer of the conductive carbon layers and the valence state transition of the polyvalent vanadium center in charge storage processes. Additionally, the custom flexible sensing patch presents an excellent sensitivity retention rate under bending (95%) and twisting (93%) strains and possesses good anti-interference performance (Δ<i>E</i> < 8 mV) against common interfering ions and organic substances in sweat. Real-time monitoring of the Cl<sup>–</sup> concentration in sweat aligns with ion chromatography analysis results. This study presents a compact wearable Cl<sup>–</sup> monitoring platform for the easy tracking of exercise-induced dehydration and cystic fibrosis screening with promising applications in smart healthcare.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"52 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-26DOI: 10.1021/acssensors.4c02772
Karley J. L. Zimmer, Ryan E. Johnson, Hunter Little, Jean Duhamel, Richard A. Manderville
DNA aptamers can bind small molecule ligands with high affinity and specificity to produce a unique supramolecular structure. Methods to obtain structural information about the binding interaction coupled with sensitive diagnostics is a gold standard for aptasensor design. However, most sensing strategies afford ligand detection without structural insight, while NMR- or crystallography-based structural methods lack sensitivity required for diagnostics. FRET-based strategies can afford both, especially with internal fluorescent nucleobase probes that are spatially fixed within the helix, but dual aptamer labeling can compromise aptamer affinity toward its target. Herein, we showcase a nucleobase surrogate-ligand FRET-based strategy that affords target-site mapping combined with sensitive target detection that addresses these challenges. A fluorescent molecular rotor (FMR) thiophene chalcone (Th6HI) nucleobase surrogate was incorporated into the tetracycline (TC) 42-mer DNA binding aptamer OTC2 to serve as an acceptor for the TC donor. Time-resolved fluorescence anisotropy experiments predict a compact prefolded OTC2 aptamer that is hardly impacted by TC binding. Consequently, direct excitation of the internal FMR Th6HI at 530 nm affords little response to TC binding, as probe rigidity is not strongly altered. In contrast, indirect excitation of the Th6HI probe through TC donor excitation at 378 nm affords site-dependent sensitized fluorescence (Fsen) of the Th6HI acceptor to afford enhanced sensitivity for TC detection compared to a native platform, which utilizes the intrinsic TC fluorescence. Furthermore, the FRET response provides target-site mapping to build a new binding model for the TC-OTC2 complex that is akin to the three-helical structure of the hammerhead ribozyme.
{"title":"Harnessing a Fluorescent Nucleobase Surrogate for Supramolecular FRET-Aptamer Detection and Target-Site Mapping","authors":"Karley J. L. Zimmer, Ryan E. Johnson, Hunter Little, Jean Duhamel, Richard A. Manderville","doi":"10.1021/acssensors.4c02772","DOIUrl":"https://doi.org/10.1021/acssensors.4c02772","url":null,"abstract":"DNA aptamers can bind small molecule ligands with high affinity and specificity to produce a unique supramolecular structure. Methods to obtain structural information about the binding interaction coupled with sensitive diagnostics is a gold standard for aptasensor design. However, most sensing strategies afford ligand detection without structural insight, while NMR- or crystallography-based structural methods lack sensitivity required for diagnostics. FRET-based strategies can afford both, especially with internal fluorescent nucleobase probes that are spatially fixed within the helix, but dual aptamer labeling can compromise aptamer affinity toward its target. Herein, we showcase a nucleobase surrogate-ligand FRET-based strategy that affords target-site mapping combined with sensitive target detection that addresses these challenges. A fluorescent molecular rotor (FMR) thiophene chalcone (Th6HI) nucleobase surrogate was incorporated into the tetracycline (TC) 42-mer DNA binding aptamer OTC2 to serve as an acceptor for the TC donor. Time-resolved fluorescence anisotropy experiments predict a compact prefolded OTC2 aptamer that is hardly impacted by TC binding. Consequently, direct excitation of the internal FMR Th6HI at 530 nm affords little response to TC binding, as probe rigidity is not strongly altered. In contrast, indirect excitation of the Th6HI probe through TC donor excitation at 378 nm affords site-dependent sensitized fluorescence (<i>F</i><sub>sen</sub>) of the Th6HI acceptor to afford enhanced sensitivity for TC detection compared to a native platform, which utilizes the intrinsic TC fluorescence. Furthermore, the FRET response provides target-site mapping to build a new binding model for the TC-OTC2 complex that is akin to the three-helical structure of the hammerhead ribozyme.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"17 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-25DOI: 10.1021/acssensors.4c03537
Pengrui Zhu, Xiaowei Zhao, Xuanhe Chen, Ran Liu, Han Ouyang, Yiran Hu, Bojing Shi, Yubo Fan
Atherosclerosis is the main cause of ischemic stroke. It occurs as a condition that leads to thickening of the arterial blood vessel walls and narrowing of the blood vessels, which can seriously affect the normal flow of blood. Currently, the detection of arterial stenosis relies on large-scale hospital equipment like computed tomography (CT) and magnetic resonance imaging (MRI), which require specialized technicians to operate and are not convenient for daily use. In addition, stenosis affects multiple parameters of hemodynamics in the blood flow field, and relying on a single physical quantity is not sufficient to understand the blood flow field localized in the stenotic vessel. Here, we demonstrated combined sensors of pulse wave and blood flow velocity (CSPB) based on photoelectric plethysmography and an ultrasonic Doppler device. We found that when the stenosis rate increased by 30%, the amplitude difference of the pulse wave curve between the two sides of the stenosis increased by over 11%, the amplitude of the blood flow curve decreased by 8%, and the blood flow resistance increased by 11%. We also prepared silicone-based models of blood stenosis vessels to build in vitro blood flow systems and achieve more accurate simulation of vascular stenosis diseases. Based on this, we studied the pulse wave and blood flow velocity curves of CSPB under different stenosis parameters. Meanwhile, we used the finite element analysis method of fluid–structure interactions to study the pulse wave and blood flow velocity changes under different arterial stenosis conditions. This study is expected to provide theoretical and technical references for achieving noninvasive detection of cardiovascular and cerebrovascular diseases based on multisensor fusion.
{"title":"Detection of Arterial Stenosis Based on Synchronized Signals from Wearable Pulse and Blood Flow Velocity Sensors","authors":"Pengrui Zhu, Xiaowei Zhao, Xuanhe Chen, Ran Liu, Han Ouyang, Yiran Hu, Bojing Shi, Yubo Fan","doi":"10.1021/acssensors.4c03537","DOIUrl":"https://doi.org/10.1021/acssensors.4c03537","url":null,"abstract":"Atherosclerosis is the main cause of ischemic stroke. It occurs as a condition that leads to thickening of the arterial blood vessel walls and narrowing of the blood vessels, which can seriously affect the normal flow of blood. Currently, the detection of arterial stenosis relies on large-scale hospital equipment like computed tomography (CT) and magnetic resonance imaging (MRI), which require specialized technicians to operate and are not convenient for daily use. In addition, stenosis affects multiple parameters of hemodynamics in the blood flow field, and relying on a single physical quantity is not sufficient to understand the blood flow field localized in the stenotic vessel. Here, we demonstrated combined sensors of pulse wave and blood flow velocity (CSPB) based on photoelectric plethysmography and an ultrasonic Doppler device. We found that when the stenosis rate increased by 30%, the amplitude difference of the pulse wave curve between the two sides of the stenosis increased by over 11%, the amplitude of the blood flow curve decreased by 8%, and the blood flow resistance increased by 11%. We also prepared silicone-based models of blood stenosis vessels to build in vitro blood flow systems and achieve more accurate simulation of vascular stenosis diseases. Based on this, we studied the pulse wave and blood flow velocity curves of CSPB under different stenosis parameters. Meanwhile, we used the finite element analysis method of fluid–structure interactions to study the pulse wave and blood flow velocity changes under different arterial stenosis conditions. This study is expected to provide theoretical and technical references for achieving noninvasive detection of cardiovascular and cerebrovascular diseases based on multisensor fusion.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"42 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-25DOI: 10.1021/acssensors.4c03290
Eloy Povedano, Víctor Pérez-Ginés, Rebeca M. Torrente-Rodríguez, Raquel Rejas-González, Ana Montero-Calle, Alberto Peláez-García, Jaime Feliú, María Pedrero, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano
This work presents the first electroanalytical bioplatforms to track individually or simultaneously at a global level all four methylation marks involved in the DNA methylation–demethylation cycle: 5-methylcytosine (5mC) and their sequential oxidative derivatives (5-hydroxymethyl-(5hmC), 5-formyl-(5fC), and 5-carboxyl-(5caC) cytosines). The bioplatforms employed direct competitive immunoassay formats implemented on the surface of magnetic microparticles (MBs) and involved capture antibodies specific to each epimark as well as synthetic biotinylated DNA oligomers with a single epimark that were enzymatically marked with horseradish peroxidase (HRP) to perform an amperometric readout on disposable platforms for single or multiplexed detection. These new electroanalytical biotechnologies, groundbreaking from analytical and clinical perspectives, achieved attractive operational characteristics, reaching detection limits at pM levels for synthetic single epimark-bearing DNA oligomers. The developed methodology was applied to track globally all four target epimarks in a fast, simple, sensitive, and selective way while their correlation in genomic DNA extracted from paired healthy and tumor tissues of patients with colorectal cancer (CRC) was established for the first time.
{"title":"Tracking Globally 5-Methylcytosine and Its Oxidized Derivatives in Colorectal Cancer Epigenome Using Bioelectroanalytical Technologies","authors":"Eloy Povedano, Víctor Pérez-Ginés, Rebeca M. Torrente-Rodríguez, Raquel Rejas-González, Ana Montero-Calle, Alberto Peláez-García, Jaime Feliú, María Pedrero, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano","doi":"10.1021/acssensors.4c03290","DOIUrl":"https://doi.org/10.1021/acssensors.4c03290","url":null,"abstract":"This work presents the first electroanalytical bioplatforms to track individually or simultaneously at a global level all four methylation marks involved in the DNA methylation–demethylation cycle: 5-methylcytosine (5mC) and their sequential oxidative derivatives (5-hydroxymethyl-(5hmC), 5-formyl-(5fC), and 5-carboxyl-(5caC) cytosines). The bioplatforms employed direct competitive immunoassay formats implemented on the surface of magnetic microparticles (MBs) and involved capture antibodies specific to each epimark as well as synthetic biotinylated DNA oligomers with a single epimark that were enzymatically marked with horseradish peroxidase (HRP) to perform an amperometric readout on disposable platforms for single or multiplexed detection. These new electroanalytical biotechnologies, groundbreaking from analytical and clinical perspectives, achieved attractive operational characteristics, reaching detection limits at pM levels for synthetic single epimark-bearing DNA oligomers. The developed methodology was applied to track globally all four target epimarks in a fast, simple, sensitive, and selective way while their correlation in genomic DNA extracted from paired healthy and tumor tissues of patients with colorectal cancer (CRC) was established for the first time.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"1 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hydrogels have emerged as promising sensors for detecting heavy metal ions in fluids and have been extensively developed. However, monitoring of multiple target analytes in Chinese herbs remains challenging due to subtle chemical signals and the complex composition of the extracted solutions. To address these challenges, we developed a hydrogel optofluidic sensor to amplify analyte signals through strong light–matter interactions within the hydrogel. This sensing platform integrates a hydrogel film encapsulated in a whispering-gallery-mode (WGM) microcavity for the detection of heavy metal ions, such as Pb2+ and Hg2+. The 3D cross-linked hydrophilic polymer network facilitates ion penetration from analyte solutions, inducing distinct WGM resonance shifts. The red shift in the spectral wavelength serves as a parameter to quantify the content of heavy metal ions. By modification of the hydrogel with aptamers, the optofluidic sensors achieve high sensitivity and selectivity. Finally, the platform’s performance was demonstrated using Chinese herbs with varying Pb2+ concentrations, highlighting its practical applicability in real-world scenarios. The proposed hydrogel microcavity exhibit a promising method for development of functional hydrogel sensors and healthcare applications.
{"title":"High-Sensitive Hydrogel Optofluidic Microcavities for Heavy Metal Ion Detection","authors":"Ruijie Wu, Ziyihui Wang, Yaoxin Fu, Junfeng Jiang, Yu-Cheng Chen, Tiegen Liu","doi":"10.1021/acssensors.5c00103","DOIUrl":"https://doi.org/10.1021/acssensors.5c00103","url":null,"abstract":"Hydrogels have emerged as promising sensors for detecting heavy metal ions in fluids and have been extensively developed. However, monitoring of multiple target analytes in Chinese herbs remains challenging due to subtle chemical signals and the complex composition of the extracted solutions. To address these challenges, we developed a hydrogel optofluidic sensor to amplify analyte signals through strong light–matter interactions within the hydrogel. This sensing platform integrates a hydrogel film encapsulated in a whispering-gallery-mode (WGM) microcavity for the detection of heavy metal ions, such as Pb<sup>2+</sup> and Hg<sup>2+</sup>. The 3D cross-linked hydrophilic polymer network facilitates ion penetration from analyte solutions, inducing distinct WGM resonance shifts. The red shift in the spectral wavelength serves as a parameter to quantify the content of heavy metal ions. By modification of the hydrogel with aptamers, the optofluidic sensors achieve high sensitivity and selectivity. Finally, the platform’s performance was demonstrated using Chinese herbs with varying Pb<sup>2+</sup> concentrations, highlighting its practical applicability in real-world scenarios. The proposed hydrogel microcavity exhibit a promising method for development of functional hydrogel sensors and healthcare applications.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"187 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-25DOI: 10.1021/acssensors.4c03619
Yanan Liu, Jiehan Lin, Junjie Wei, Tao Chen, Wenqin Wang
Flexible wearable electronic devices based on hydrophobic, conductive hydrogels have attracted widespread attention in the field of underwater sensing. However, traditional homogeneous hydrogels tend to compromise their conductivity and sensing performance when achieving hydrophobicity, and the high complexity of marine environments further reduces their sensing performance and service life. Here, we develop a seawater-resistant conductive hydrogel with ultrahigh sensitivity and self-healing ability by the introduction of a skin-like heterogeneous structure, consisting of a hydrophobic outer layer that protects against seawater and a conductive internal layer that senses. Based on a heterogeneous structure obtained through surface hydrophobic modification of confined nitrogen-alkylation reaction, the conductive hydrogel simultaneously achieves satisfying seawater resistance (contact angle of 123.2°), high ionic conductivity (2.86 S m–1), and excellent sensing sensitivity in seawater (GF = 6.15), harmonizing the contradiction between water resistance and sensing of traditional hydrophobic hydrogels. In addition, abundant hydrogen-bonding and dipole–dipole interactions endow the heterogeneous hydrogel with an outstanding self-healing ability, exhibiting high-efficiency self-healing behavior in seawater. Underwater strain sensors constructed with the heterogeneous hydrogel can be used for detecting human motion in simulated seawater environments and real-time signal transmission, showcasing their great potential as wearable electronic devices in the marine sensing field.
{"title":"Skin-like Heterogeneous and Self-Healing Conductive Hydrogel toward Ultrasensitive Marine Sensing","authors":"Yanan Liu, Jiehan Lin, Junjie Wei, Tao Chen, Wenqin Wang","doi":"10.1021/acssensors.4c03619","DOIUrl":"https://doi.org/10.1021/acssensors.4c03619","url":null,"abstract":"Flexible wearable electronic devices based on hydrophobic, conductive hydrogels have attracted widespread attention in the field of underwater sensing. However, traditional homogeneous hydrogels tend to compromise their conductivity and sensing performance when achieving hydrophobicity, and the high complexity of marine environments further reduces their sensing performance and service life. Here, we develop a seawater-resistant conductive hydrogel with ultrahigh sensitivity and self-healing ability by the introduction of a skin-like heterogeneous structure, consisting of a hydrophobic outer layer that protects against seawater and a conductive internal layer that senses. Based on a heterogeneous structure obtained through surface hydrophobic modification of confined nitrogen-alkylation reaction, the conductive hydrogel simultaneously achieves satisfying seawater resistance (contact angle of 123.2°), high ionic conductivity (2.86 S m<sup>–1</sup>), and excellent sensing sensitivity in seawater (GF = 6.15), harmonizing the contradiction between water resistance and sensing of traditional hydrophobic hydrogels. In addition, abundant hydrogen-bonding and dipole–dipole interactions endow the heterogeneous hydrogel with an outstanding self-healing ability, exhibiting high-efficiency self-healing behavior in seawater. Underwater strain sensors constructed with the heterogeneous hydrogel can be used for detecting human motion in simulated seawater environments and real-time signal transmission, showcasing their great potential as wearable electronic devices in the marine sensing field.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"8 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143486594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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