The objective of this study was to construct appropriate stem taper functions at the individual tree level of Acacia hybrid plantations. The stem taper functions were constructed from 150 sample trees at the age of 3 - 10 years in which their diameter at breast height ranged from 4 - 24 cm. The appropriate stem taper functions were constructed and tested from 2 forms of candidate functions. The form 1 was a multivariable stem taper function and form 2 was a single-variable stem taper function. The research results showed that the deviation of the multivariable outsidebark stem taper function was 19.0% and 42.2% smaller than that of the single-variable function of order 7 and order 2, respectively. Similarly, the deviation of the multivariable inside-bark stem taper function was 13.6% and 32.8% smaller than that of the single-variable stem taper function of order 7 and order 2, respectively. Accuracy of multi-order single-variable stem taper functions was improved by constructing for each diameter class.
{"title":"Modeling the stem taper functions for Acacia hybrid in Dong Nai province","authors":"Them V. Nguyen","doi":"10.52997/jad.2.04.2023","DOIUrl":"https://doi.org/10.52997/jad.2.04.2023","url":null,"abstract":"The objective of this study was to construct appropriate stem taper functions at the individual tree level of Acacia hybrid plantations. The stem taper functions were constructed from 150 sample trees at the age of 3 - 10 years in which their diameter at breast height ranged from 4 - 24 cm. The appropriate stem taper functions were constructed and tested from 2 forms of candidate functions. The form 1 was a multivariable stem taper function and form 2 was a single-variable stem taper function. The research results showed that the deviation of the multivariable outsidebark stem taper function was 19.0% and 42.2% smaller than that of the single-variable function of order 7 and order 2, respectively. Similarly, the deviation of the multivariable inside-bark stem taper function was 13.6% and 32.8% smaller than that of the single-variable stem taper function of order 7 and order 2, respectively. Accuracy of multi-order single-variable stem taper functions was improved by constructing for each diameter class.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"199 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117093958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to select Arabidopsis seeds after transformation with the AtZAT12 gene via Agrobacterium tumerfaciens. ZAT12 is a transcription factor that inhibits other transcription factors FIT through the EAR motif. FIT itself is a central transcription factor that controls Fe uptake under Fe-deficient conditions. However, if Fe is absorbed excessively, it will produce reactive oxygen species that could damage cells. That is the reason why AtZAT12 transcription is elevated and FIT transcription is inhibited under Fe deficiency for 10 days. To investigate the function, the AtZAT12 gene was inserted into the pMDC107 vector for transformation into Arabidopsis. The T0 Arabidopsis seeds obtained after floral dip transformation were placed on 1% MS agar supplemented with 15 μg/mL Hygromycin B. Beforehand, the T0 seeds were sterilized and kept in the dark for stratification. Subsequently, the seed plates were subjected to a regime of 6 h of light, 48 h of dark and 24 h of light (3.25 d). The hygromycin B-resistant seedlings had long hypocotyls (~ 1.0 cm), while the non-resistant seedlings had short (~ 0.3 cm) hypocotyls. This method took only 3.25 days to identify transgenic Arabidopsis seedlings. After that, positive transgene lines were examined by PCR method for AtZAT12, and the expression of AtZAT12 was observed under microscope.
{"title":"Selection of Arabidopsis transformants containing AtZAT12","authors":"C. T. T. Le, N. T. Pham","doi":"10.52997/jad.4.04.2023","DOIUrl":"https://doi.org/10.52997/jad.4.04.2023","url":null,"abstract":"This study aimed to select Arabidopsis seeds after transformation with the AtZAT12 gene via Agrobacterium tumerfaciens. ZAT12 is a transcription factor that inhibits other transcription factors FIT through the EAR motif. FIT itself is a central transcription factor that controls Fe uptake under Fe-deficient conditions. However, if Fe is absorbed excessively, it will produce reactive oxygen species that could damage cells. That is the reason why AtZAT12 transcription is elevated and FIT transcription is inhibited under Fe deficiency for 10 days. To investigate the function, the AtZAT12 gene was inserted into the pMDC107 vector for transformation into Arabidopsis. The T0 Arabidopsis seeds obtained after floral dip transformation were placed on 1% MS agar supplemented with 15 μg/mL Hygromycin B. Beforehand, the T0 seeds were sterilized and kept in the dark for stratification. Subsequently, the seed plates were subjected to a regime of 6 h of light, 48 h of dark and 24 h of light (3.25 d). The hygromycin B-resistant seedlings had long hypocotyls (~ 1.0 cm), while the non-resistant seedlings had short (~ 0.3 cm) hypocotyls. This method took only 3.25 days to identify transgenic Arabidopsis seedlings. After that, positive transgene lines were examined by PCR method for AtZAT12, and the expression of AtZAT12 was observed under microscope.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132576458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objectives of this study were to evaluate the status of pyometra in dogs and effects of treatment, and to isolate bacteria from the inflammatory fluid of pyometra in dogs. The study was carried out from September 2022 to February 2023 at the Animal Health Laboratory and Treatment Division, Sub-Department of Animal Health and Husbandry of Ho Chi Minh City. Out of total 1,715 bitches, there were 81 cases of pyometra (4.43%). The results showed that dogs aged 5 - 7 years (35.80%) had the highest rate of pyometra, followed by dogs aged 2 - 4 years (20.99%), and the lowest rate with dogs < 2 years old (3.70%). Local breeds had a lower pyometra rate (19.75%) compared to foreign breeds (80.25%) (P < 0.05). Dogs kept in cages accounted for 58.02% of the pyometra rate, which was higher than the rate of free-roaming dogs at 41.98% (P < 0.05). Non-reproductive dogs had the highest rate of this disease (64.20%), followed by primiparous dogs (25.93%) and the lowest rate was recorded in dogs with parity 5 (1.23%) (P < 0.05). Common clinical symptoms included moodiness/proneness, vaginal discharge, abdominal distension/pain to touch, excessive drinking/thirst, loss of appetite, dehydration, fever, weight loss, polyuria and vomiting, which were important for the diagnosis of pyometra. Streptococcus was a bacterial group found in uterine fluid samples of pyometra by isolation and culture method. The treatment method chosen by the owners for pyometra in dogs in Ho Chi Minh City was surgical intervention and the effect of successful treatment was very high (90.12%).
{"title":"Investigation of pyometra in dogs in Ho Chi Minh City, Vietnam","authors":"H. T. Pham, Thuong-Thuong Nguyen","doi":"10.52997/jad.6.04.2023","DOIUrl":"https://doi.org/10.52997/jad.6.04.2023","url":null,"abstract":"The objectives of this study were to evaluate the status of pyometra in dogs and effects of treatment, and to isolate bacteria from the inflammatory fluid of pyometra in dogs. The study was carried out from September 2022 to February 2023 at the Animal Health Laboratory and Treatment Division, Sub-Department of Animal Health and Husbandry of Ho Chi Minh City. Out of total 1,715 bitches, there were 81 cases of pyometra (4.43%). The results showed that dogs aged 5 - 7 years (35.80%) had the highest rate of pyometra, followed by dogs aged 2 - 4 years (20.99%), and the lowest rate with dogs < 2 years old (3.70%). Local breeds had a lower pyometra rate (19.75%) compared to foreign breeds (80.25%) (P < 0.05). Dogs kept in cages accounted for 58.02% of the pyometra rate, which was higher than the rate of free-roaming dogs at 41.98% (P < 0.05). Non-reproductive dogs had the highest rate of this disease (64.20%), followed by primiparous dogs (25.93%) and the lowest rate was recorded in dogs with parity 5 (1.23%) (P < 0.05). Common clinical symptoms included moodiness/proneness, vaginal discharge, abdominal distension/pain to touch, excessive drinking/thirst, loss of appetite, dehydration, fever, weight loss, polyuria and vomiting, which were important for the diagnosis of pyometra. Streptococcus was a bacterial group found in uterine fluid samples of pyometra by isolation and culture method. The treatment method chosen by the owners for pyometra in dogs in Ho Chi Minh City was surgical intervention and the effect of successful treatment was very high (90.12%).","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134245262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The greater wax moth (Galleria mellonella L.) is a common insect used as alternative host for mass rearing and research of natural enemies. Finding appropriate artificial diets can aid in increasing the efficiency of mass rearing. The study evaluated three different artificial diets by determining some biological and morphological characteristics of greater wax moths in laboratory of Plant Protection Department, Agronomy Faculty, Nong Lam University, Ho Chi Minh City, Vietnam. The experimental results showed that the artificial diet CT2 was the most effective one of the three treatments. Its larval stage was shortest, with an average of 27.6 ± 4.2 days. The greater wax moth larvae reared on CT2 grew fast and had large size. Over a period of 20 - 30 days of age, the length and width of greater wax moth larvae ranged from 11.9 ± 5.2 to 16.3 ± 6.2 mm and 2.7 ± 1.4 to 3.9 ± 1.8 mm, respectively. The female greater wax moths laid 819.0 ± 175.5 to 1,008.1 ± 354.6 eggs on average. When greater wax moths were reared on three different artificial diets, there was no difference in fecundity
{"title":"Morphological and biological characteristics of Galleria mellonella L. (Lepidoptera: Pyralidae) reared on different artificial diets","authors":"H. K. Le, Dong T. H. Tran","doi":"10.52997/jad.1.04.2023","DOIUrl":"https://doi.org/10.52997/jad.1.04.2023","url":null,"abstract":"The greater wax moth (Galleria mellonella L.) is a common insect used as alternative host for mass rearing and research of natural enemies. Finding appropriate artificial diets can aid in increasing the efficiency of mass rearing. The study evaluated three different artificial diets by determining some biological and morphological characteristics of greater wax moths in laboratory of Plant Protection Department, Agronomy Faculty, Nong Lam University, Ho Chi Minh City, Vietnam. The experimental results showed that the artificial diet CT2 was the most effective one of the three treatments. Its larval stage was shortest, with an average of 27.6 ± 4.2 days. The greater wax moth larvae reared on CT2 grew fast and had large size. Over a period of 20 - 30 days of age, the length and width of greater wax moth larvae ranged from 11.9 ± 5.2 to 16.3 ± 6.2 mm and 2.7 ± 1.4 to 3.9 ± 1.8 mm, respectively. The female greater wax moths laid 819.0 ± 175.5 to 1,008.1 ± 354.6 eggs on average. When greater wax moths were reared on three different artificial diets, there was no difference in fecundity","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123131029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The experiment was conducted from August 2020 to March 2022 at the Veterinary Hospital of Nong Lam University, Ho Chi Minh City. The aim of this study was to isolate and analyze Tembusu virus gene (flavivirus) using 9 to 11 day-old embryonic duck eggs. Treated specimens were inoculated into embryonic duck eggs by allantoic route, then eggs were observed every day for 7 days. The allantoic fluids of the survival eggs were inoculated up to 3 times. Subsequently, the allantoic fluids were checked by real-time RT-PCR to detect Tembusu virus. A positive sample was then titrated, and finally a partial genome was sequenced as well as a phylogenetic tree was constructed. As a result, a strain of Tembusu virus was isolated, and genetic analysis of partial E gene showed that this virus strain was highly similar (from 96,8% to 98,15%) to others in China and Thailand.
{"title":"Isolation and genetic analysis of Tembusu virus in ducks","authors":"Dat M. Truong, H. Hoang","doi":"10.52997/jad.5.04.2023","DOIUrl":"https://doi.org/10.52997/jad.5.04.2023","url":null,"abstract":"The experiment was conducted from August 2020 to March 2022 at the Veterinary Hospital of Nong Lam University, Ho Chi Minh City. The aim of this study was to isolate and analyze Tembusu virus gene (flavivirus) using 9 to 11 day-old embryonic duck eggs. Treated specimens were inoculated into embryonic duck eggs by allantoic route, then eggs were observed every day for 7 days. The allantoic fluids of the survival eggs were inoculated up to 3 times. Subsequently, the allantoic fluids were checked by real-time RT-PCR to detect Tembusu virus. A positive sample was then titrated, and finally a partial genome was sequenced as well as a phylogenetic tree was constructed. As a result, a strain of Tembusu virus was isolated, and genetic analysis of partial E gene showed that this virus strain was highly similar (from 96,8% to 98,15%) to others in China and Thailand.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114347303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to assess the efficiency of Nile and red tilapia cultured in intensive systems applied a combination of biofloc technology (BFT) and probiotics in earthen ponds with different methods to control the off-flavor problem. A growing-out experiment was conducted for 150 days in which both Nile tilapia (5.88 ± 0.59 g) and red tilapia (9.72 ± 0.72 g) fingerlings were randomly allotted into three replicates of earthen ponds which were subdivided into four treatment groups, including: off-flavor controlled by algae management in Nile tilapia ponds (NT - AM) and red tilapia ponds (RT - AM), and off-flavor controlled by active water exchange in Nile tilapia ponds (NT - WE) and red tilapia ponds (RT - WE). The results showed that water quality was maintained in suitable ranges for fish growth. There were no significant differences in growth performance (final weight, daily weight gain and specific growth rate) among all treatments (P < 0.05). Feed conversion ratio of Nile tilapia (1.28 - 1.31) was significantly lower than that of red tilapia (1.35 - 1.37) (P < 0.05). The survival ratio (about 80%) and extrapolated yield (21.50 tons/ha in 150 days) of fish was similar and high for both Nile and red tilapia. The quality of the harvested fish in term of condition factor, size even and off-flavor intensity was also excellent. The economic efficiency of red tilapia farming in this system was higher as compared to cage systems. This study clearly demonstrated that the technique of combined BFT and probiotic application in earthen ponds could contribute to the sustainable development of tilapia production in Vietnam by reducing production cost, saving water resource and avoiding environmental pollution.
{"title":"Efficiency of intensive tilapia culture in earthen ponds applied biofloc technology (BFT), probiotics and off-flavor control methods","authors":"Loan P. Phan, Tuat V. Nguyen","doi":"10.52997/jad.2.03.2023","DOIUrl":"https://doi.org/10.52997/jad.2.03.2023","url":null,"abstract":"The aim of this study was to assess the efficiency of Nile and red tilapia cultured in intensive systems applied a combination of biofloc technology (BFT) and probiotics in earthen ponds with different methods to control the off-flavor problem. A growing-out experiment was conducted for 150 days in which both Nile tilapia (5.88 ± 0.59 g) and red tilapia (9.72 ± 0.72 g) fingerlings were randomly allotted into three replicates of earthen ponds which were subdivided into four treatment groups, including: off-flavor controlled by algae management in Nile tilapia ponds (NT - AM) and red tilapia ponds (RT - AM), and off-flavor controlled by active water exchange in Nile tilapia ponds (NT - WE) and red tilapia ponds (RT - WE). The results showed that water quality was maintained in suitable ranges for fish growth. There were no significant differences in growth performance (final weight, daily weight gain and specific growth rate) among all treatments (P < 0.05). Feed conversion ratio of Nile tilapia (1.28 - 1.31) was significantly lower than that of red tilapia (1.35 - 1.37) (P < 0.05). The survival ratio (about 80%) and extrapolated yield (21.50 tons/ha in 150 days) of fish was similar and high for both Nile and red tilapia. The quality of the harvested fish in term of condition factor, size even and off-flavor intensity was also excellent. The economic efficiency of red tilapia farming in this system was higher as compared to cage systems. This study clearly demonstrated that the technique of combined BFT and probiotic application in earthen ponds could contribute to the sustainable development of tilapia production in Vietnam by reducing production cost, saving water resource and avoiding environmental pollution.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128832577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vung Tau city is an area where management of land use planning (LUP) and urban planning is quite complicated and has been successful in building LUP database. However, this database still has limitations that must be addressed, including (1) lack of data layer on current land use for building LUP database, (2) incompleted LUP attribute database, (3) lack of metadata, (4) no connection between LUP database and digital legal materials and (5) no overlay cadastral database to data layer of current land use and LUP database. To solve these problems, this research used many methods such as: documents and data collection, inheritance, expert interview, data processing and analysis, mapping, GIS application, and IT applications. The results of this study showed that the structure of cadastral database of 8 wards was succesfully converted from existing structure (according to Circular 17/2010/TT-BTNMT) to the standard structure (according to Circular 75/2015/TT-BTNMT). The 2019 land inventory data was standardized and data layer of current land use for whole city was built. In addition, the LUP database (period 2010 - 2020) for Vung Tau was improved, including: supplementing attribute information, completing metadata, connecting LUP database with relevant digital legal records, overlaying cadastral database with LUP database and data layer of current land use. Briefly, our results could be a foundation to help Vung Tau manage land sustainably, contribute to successfully building national land database and meet the exploitation requirements of LUP information in accordance with the orientation of Ministry of Natural Resources and Environment for study area.
{"title":"Improving the database on land use planning in Vung Tau city, Ba Ria - Vung Tau province","authors":"Linh D. Truong","doi":"10.52997/jad.6.03.2023","DOIUrl":"https://doi.org/10.52997/jad.6.03.2023","url":null,"abstract":"Vung Tau city is an area where management of land use planning (LUP) and urban planning is quite complicated and has been successful in building LUP database. However, this database still has limitations that must be addressed, including (1) lack of data layer on current land use for building LUP database, (2) incompleted LUP attribute database, (3) lack of metadata, (4) no connection between LUP database and digital legal materials and (5) no overlay cadastral database to data layer of current land use and LUP database. To solve these problems, this research used many methods such as: documents and data collection, inheritance, expert interview, data processing and analysis, mapping, GIS application, and IT applications. The results of this study showed that the structure of cadastral database of 8 wards was succesfully converted from existing structure (according to Circular 17/2010/TT-BTNMT) to the standard structure (according to Circular 75/2015/TT-BTNMT). The 2019 land inventory data was standardized and data layer of current land use for whole city was built. In addition, the LUP database (period 2010 - 2020) for Vung Tau was improved, including: supplementing attribute information, completing metadata, connecting LUP database with relevant digital legal records, overlaying cadastral database with LUP database and data layer of current land use. Briefly, our results could be a foundation to help Vung Tau manage land sustainably, contribute to successfully building national land database and meet the exploitation requirements of LUP information in accordance with the orientation of Ministry of Natural Resources and Environment for study area.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121520393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of the study was to evaluate the use of loop-mediated isothermal amplification (LAMP) technique for detection of Salmonella spp. in chicken eggs. Eight Salmonella strains and 26 non-Salmonella enteric strains were used to verify the specificity of the LAMP assay for detection of Salmonella. The Sal4 primer set was used to specifically amplify the target sequence on the invA gene of Salmonella and the hydroxyl naphthol blue was used as the indicator to read the reaction results. Blank egg samples were spiked with serial 10-fold diluted Salmonella suspensions to determine the limit of detection at 50% (LOD50) of the assay. Egg samples collected from multiple locations in Ho Chi Minh City were tested for the presence of Salmonella using both LAMP method and the traditional culture method to compare the effectiveness of these two methods. Among 34 bacterial strains tested by LAMP assay, false-positive or false-negative results were not observed. The assays of spiked egg samples showed that the LOD50 of the LAMP method was less than 2 CFU/25 g sample. These results indicated a high specificity and sensitivity of the LAMP assay in detection of Salmonella in egg samples, even with those of low levels of contamination. Upon testing collected egg samples (n = 42), the LAMP assay produced the same results of Salmonella detection as the culture method. Salmonella was detected in 4 out of 42 samples. This study showed that the LAMP method is highly effective and would be suitable for use in detection of Salmonella in egg samples. The assay has several advantages such as saving time and labor compared to traditional culture methods.
{"title":"Application of loop-mediated isothermal amplification to detect Salmonella spp. in egg samples","authors":"Kha T. Tran, T. T. Nguyen","doi":"10.52997/jad.1.03.2023","DOIUrl":"https://doi.org/10.52997/jad.1.03.2023","url":null,"abstract":"The objective of the study was to evaluate the use of loop-mediated isothermal amplification (LAMP) technique for detection of Salmonella spp. in chicken eggs. Eight Salmonella strains and 26 non-Salmonella enteric strains were used to verify the specificity of the LAMP assay for detection of Salmonella. The Sal4 primer set was used to specifically amplify the target sequence on the invA gene of Salmonella and the hydroxyl naphthol blue was used as the indicator to read the reaction results. Blank egg samples were spiked with serial 10-fold diluted Salmonella suspensions to determine the limit of detection at 50% (LOD50) of the assay. Egg samples collected from multiple locations in Ho Chi Minh City were tested for the presence of Salmonella using both LAMP method and the traditional culture method to compare the effectiveness of these two methods. Among 34 bacterial strains tested by LAMP assay, false-positive or false-negative results were not observed. The assays of spiked egg samples showed that the LOD50 of the LAMP method was less than 2 CFU/25 g sample. These results indicated a high specificity and sensitivity of the LAMP assay in detection of Salmonella in egg samples, even with those of low levels of contamination. Upon testing collected egg samples (n = 42), the LAMP assay produced the same results of Salmonella detection as the culture method. Salmonella was detected in 4 out of 42 samples. This study showed that the LAMP method is highly effective and would be suitable for use in detection of Salmonella in egg samples. The assay has several advantages such as saving time and labor compared to traditional culture methods.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127621457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant growth promoting Rhizobacteria (PGPR) have long been selected as biocontrol agents for plants. The PGPR are beneficial bacteria that live in plant roots and enhance plant growth by various of mechanisms. The PGPR have many species of the bacterial genus Pseudomonas, in which the Pseudomonas fluorescens strains is suitable for application as a biocontrol agent due to its abundance in natural soil and plant root systems. Pseudomonas fluorescens strains have important characteristics such as the ability to adhere to soil particles and to rhizomes, the ability to synthesize antibiotics, and to produce hydrolytic enzymes. Furthermore, P. fluorescens strains also possess plant growth promoting characteristics such as proteolysis, phosphate solubilization, iron chelation, and phytohormone production. Durian is a crop that has been expanded in Southeast Asian countries but the rate of durians infected with root, stem and fruit rot caused by P. palmivora is quite large. In this study, five native P. fluorescens isolates were isolated from 70 soil and rhizome samples collected from 10 durian growing provinces and evaluated for their ability to antagonize P. palmivora in petri plates. These isolates were gram negative, small, single isolated rods without sporulation when observed under microscope. All of them tested positive for catalase test, oxidase test, starch hydorolysis, gelatine liquefaction, H2S, citrate utilization and negative with indole, Voges-proskauers, methyl red. All of 5 native P. fluorescens isolates were capable of phosphate solubilizing activity, N2 fixation, siderophore and IAA production. The study showed that P. fluorescens P. fDN strain was able to inhibit P. palmivora causing rot disease in durian with 51.85% inhibition of radial growth.
促进植物生长的根瘤菌(Rhizobacteria, PGPR)一直是植物的生物防治剂。PGPR是生活在植物根系中的有益菌，通过多种机制促进植物生长。假单胞菌属细菌种类繁多，其中荧光假单胞菌菌株在自然土壤和植物根系中含量丰富，适合作为生物防治剂应用。荧光假单胞菌菌株具有一些重要的特性，例如能够附着在土壤颗粒和根茎上，能够合成抗生素，并产生水解酶。此外，荧光假单胞菌菌株还具有促进植物生长的特性，如蛋白质水解、磷酸盐溶解、铁螯合和植物激素的产生。榴莲是东南亚国家推广的一种作物，但由棕榈芽孢杆菌引起的根、茎、果腐病在榴莲中的发病率相当高。本研究从10个榴莲种植区的70份土壤和根茎中分离出5株荧光假单孢菌，并在培养皿中对其拮抗棕榈假单孢菌的能力进行了评价。这些分离株在显微镜下观察为革兰氏阴性，小的，单分离的杆状体，没有孢子。过氧化氢酶、氧化酶、淀粉水解、明胶液化、H2S、柠檬酸利用均呈阳性，吲哚、Voges-proskauers、甲基红均呈阴性。5株荧光假单胞菌均具有溶磷活性、固氮活性、铁载体活性和IAA活性。研究表明，荧光假单胞菌(P. fluorescens P. fDN)能抑制棕榈假单胞菌(P. palmivora)引起的榴莲腐病，其径向生长抑制率为51.85%。
{"title":"Isolation and characteristics of Pseudomonas fluorescens to inhibit Phytophthora palmivora causing rot disease in durian","authors":"Van-Tuan Tran, H. Nguyen, H. T. Nguyen, Don D. Le","doi":"10.52997/jad.4.03.2023","DOIUrl":"https://doi.org/10.52997/jad.4.03.2023","url":null,"abstract":"Plant growth promoting Rhizobacteria (PGPR) have long been selected as biocontrol agents for plants. The PGPR are beneficial bacteria that live in plant roots and enhance plant growth by various of mechanisms. The PGPR have many species of the bacterial genus Pseudomonas, in which the Pseudomonas fluorescens strains is suitable for application as a biocontrol agent due to its abundance in natural soil and plant root systems. Pseudomonas fluorescens strains have important characteristics such as the ability to adhere to soil particles and to rhizomes, the ability to synthesize antibiotics, and to produce hydrolytic enzymes. Furthermore, P. fluorescens strains also possess plant growth promoting characteristics such as proteolysis, phosphate solubilization, iron chelation, and phytohormone production. Durian is a crop that has been expanded in Southeast Asian countries but the rate of durians infected with root, stem and fruit rot caused by P. palmivora is quite large. In this study, five native P. fluorescens isolates were isolated from 70 soil and rhizome samples collected from 10 durian growing provinces and evaluated for their ability to antagonize P. palmivora in petri plates. These isolates were gram negative, small, single isolated rods without sporulation when observed under microscope. All of them tested positive for catalase test, oxidase test, starch hydorolysis, gelatine liquefaction, H2S, citrate utilization and negative with indole, Voges-proskauers, methyl red. All of 5 native P. fluorescens isolates were capable of phosphate solubilizing activity, N2 fixation, siderophore and IAA production. The study showed that P. fluorescens P. fDN strain was able to inhibit P. palmivora causing rot disease in durian with 51.85% inhibition of radial growth.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130692943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viet B. Nguyen, H. T. Nguyen, G. Ho, H. T. Nguyen, Suong T. T. Nguyen, Dao N. Duy, Sang M. Nguyen
Saltwater intrusion is a common phenomenon in Southern Vietnam, but salinization is becoming more serious due to the rising sea level related to climate change. Among potential methods for water desalination, the application of halophytic microalgae is gaining high interest. This study investigated the effect of Chlorella vulgaris (C. vulgaris) microalgae on reducing water salinity at different media (Bold Basal Medium and Sea Salt Medium) and in different salt concentrations (1 - 30 g/L). The results indicated that C. vulgaris microalgae had good growth in all mediums used and contributed to lowering the salt content from 20% to 40% after 15 days of cultivation.
在越南南部，海水入侵是一种常见的现象，但由于气候变化导致海平面上升，盐碱化正变得越来越严重。在潜在的海水淡化方法中，盐生微藻的应用备受关注。本研究考察了小球藻(Chlorella vulgaris, C. vulgaris)微藻在不同培养基(Bold基础培养基和海盐培养基)和不同盐浓度(1 ~ 30 g/L)下降低水体盐度的效果。结果表明，微藻在所有培养基中均有良好的生长，培养15 d后将含盐量从20%降低到40%。
{"title":"Water desalination of Chlorella vulgaris","authors":"Viet B. Nguyen, H. T. Nguyen, G. Ho, H. T. Nguyen, Suong T. T. Nguyen, Dao N. Duy, Sang M. Nguyen","doi":"10.52997/jad.5.03.2023","DOIUrl":"https://doi.org/10.52997/jad.5.03.2023","url":null,"abstract":"Saltwater intrusion is a common phenomenon in Southern Vietnam, but salinization is becoming more serious due to the rising sea level related to climate change. Among potential methods for water desalination, the application of halophytic microalgae is gaining high interest. This study investigated the effect of Chlorella vulgaris (C. vulgaris) microalgae on reducing water salinity at different media (Bold Basal Medium and Sea Salt Medium) and in different salt concentrations (1 - 30 g/L). The results indicated that C. vulgaris microalgae had good growth in all mediums used and contributed to lowering the salt content from 20% to 40% after 15 days of cultivation.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127770411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：