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Qualitative analysis of insecticide residue in cauliflower samples collected from different regions of Bangladesh 孟加拉不同地区花椰菜中杀虫剂残留的定性分析
Pub Date : 2018-05-31 DOI: 10.3329/aajfss.v2i1.55896
M. Ahmed, Md Mohsin Ali Sardar, Masum Ahmad, K. Kabir
The samples of the cauliflowers were analyzed to assess the residue level of six insecticides (i.e.; cypermethrin, quinalphos, diazinon, malathion, fenitrothion and acephate) using GC-FTD and GC-ECD method. Out of analyzed 75 collected samples of cauliflower from farmer’s field of Jessore, Gazipur and Rangpur, 38.67% (29 from 75) were found to be contaminated with the insecticides. Many samples contained cypermethrin, acephate and fenitrothion residues. Quinalphos, acephate and fenitrothion were found as multiple product residues representing 13.79% (4 from 29) of the total contaminated samples and the rest 86.21% contained single insecticide residue. About 13.33% (10 of 75) of the total samples had residues exceeding the MRL (Maximum Residue Limit) irrespective of single or multiple insecticide residues. Malathion residue was found only in one sample from Jessore. Two samples showed diazinon residue one from Jessore and the other one from Rangpur location. The detected residue levels of both malathion and diazinon were below the MRL, which might be due to higher rate of degradation. The presence of highest residue levels of insecticides in cauliflowers may be due to its irrational and repeated use before harvest. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 29-34
对菜花样品进行分析,评价6种杀虫剂(即;氯氰菊酯、喹硫磷、二嗪磷、马拉硫磷、吡硫磷和乙酰甲胺磷),采用GC-FTD和GC-ECD方法。从杰索尔、加济布尔和朗布尔农民田间采集的75份花椰菜样品中,发现38.67%(75份中有29份)被杀虫剂污染。许多样品含有氯氰菊酯、甲胺磷和杀虫硫磷残留。喹磷、甲胺磷和杀虫硫磷为多重残留,占污染样品总数的13.79%(29份中4份),其余86.21%为单一残留。75份样品中有10份(13.33%)的农药残留超过最大残留限量(MRL)。只在杰索尔的一个样本中发现了马拉硫磷残留。两个样本显示有重氮农残留一个来自杰索尔,另一个来自朗布尔。马拉硫磷和二嗪磷的检测残留量均低于MRL,这可能是由于降解率较高。菜花中杀虫剂残留量最高可能是由于收获前不合理和重复使用造成的。亚洲Australas。J.食品安全。安全,2018,2(1),29-34
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引用次数: 5
Effect of egg level on buffalo milk pudding 鸡蛋水平对水牛奶布丁的影响
Pub Date : 2018-05-31 DOI: 10.3329/aajfss.v2i1.55898
A. Rahman, M. Sarker, N. Sultana, S. Sultana
The experiment was conducted to determine the optimum level of egg in the manufacture of buffalo milk pudding. Three types of pudding were prepared using different levels of eggs (40%, 30% and 20% by weight) and a constant level of corn flour (4%) at Dairy Technology and Microbiology Laboratory of the Department of Dairy Science, Bangladesh Agricultural University, Mymensingh. Quality of pudding was evaluated by physical, chemical and microbial test. From the result of physical study (smell and taste, body and consistency, color and appearance) it was found that pudding of 40% egg obtained the best score (97.50±3.77) followed by 30% (83.96±2.52) and 20% egg containing pudding (74.93±1.01). The differences within the overall score with different level of egg had significant effect. Chemical analysis showed the significant differences (p<0.01) within the total solids (TS) (340.61±1.72, 334.18±2.10 and 327.48±2.61), moisture (659.39±1.72, 665.82±2.10 and 672.52±2.61), fat (79.37±1.90, 76.43±2.76 and 73.47±3.62), protein (60.43±0.90, 53.82±0.57 and 47.07±0.25), carbohydrate (194.02±1.07, 197.33±1.17 and 200.57±1.21) and pH (6.90±0.00, 6.93±0.21 and 6.87±0.22) but no significant differences was found in ash (6.78±0.14, 6.58±0.13 and 6.38±0.01) and acidity percentage (0.071±0.00, 0.071±0.00 and 0.071±0.00%). Microbial analysis showed that there were significant differences (p<0.01) in total bacterial count (43.00±2.65×104, 38.33±1.53×104 and 35.00±3.00×104) in the pudding containing different levels of eggs. The experiment concluded that the egg level has a significant effect on the buffalo milk. The better result for buffalo pudding is at 40% egg level by weight with 4% constant corn flour. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 35-39 
本试验旨在确定水牛奶布丁中鸡蛋的最佳添加量。在Mymensingh孟加拉国农业大学乳制品科学系的乳制品技术和微生物实验室,使用不同水平的鸡蛋(重量40%、30%和20%)和恒定水平的玉米粉(4%)制备了三种布丁。采用物理、化学、微生物等试验方法评价布丁的品质。从理化指标(香与味、体与稠度、色与形)分析结果来看,含40%鸡蛋的布丁得分最高(97.50±3.77),其次是含30%鸡蛋的布丁(83.96±2.52)和含20%鸡蛋的布丁(74.93±1.01)。不同鸡蛋水平对整体得分的差异有显著影响。化学分析结果显示,总固形物(TS)(340.61±1.72、334.18±2.10和327.48±2.61)、水分(659.39±1.72、665.82±2.10和672.52±2.61)、脂肪(79.37±1.90、76.43±2.76和73.47±3.62)、蛋白质(60.43±0.90、53.82±0.57和47.07±0.25)、碳水化合物(194.02±1.07、197.33±1.17和200.57±1.21)和pH(6.90±0.00、6.93±0.21和6.87±0.22)差异显著(p<0.01),但灰分(6.78±0.14、(6.58±0.13和6.38±0.01)和酸度百分比(0.071±0.00、0.071±0.00和0.071±0.00%)。微生物学分析表明,不同鸡蛋添加水平的布丁细菌总数分别为43.00±2.65×104、38.33±1.53×104和35.00±3.00×104,差异有统计学意义(p<0.01)。实验结果表明,鸡蛋水平对水牛奶有显著影响。水牛水布丁的最佳效果是在40%的鸡蛋重量水平下,添加4%的恒定玉米粉。亚洲Australas。J.食品安全。安全,2018,2(1),35-39
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引用次数: 0
Isolation, molecular identification and characterization of Aeromonas hydrophila from infected air-breathing catfish Magur (Clarias batrachus) cultured in Mymensingh, Bangladesh 孟加拉国迈门辛格养殖的受感染空气呼吸鲶鱼中嗜水气单胞菌的分离、分子鉴定和特性分析
Pub Date : 2017-11-21 DOI: 10.3329/aajfss.v1i1.55757
Md Shirajum Monir, N. Bagum, S. Kabir, Shuvho Chakra Borty, Mohammad Ashaf Ud Doulah
The study was carried out from November, 2014 to February, 2015 with the objective of isolating Aeromonas hydrophila an important fish pathogen from infected air-breathing catfish Magur (Clarias batrachus) in Mymensingh district. Quantitative study of the isolated bacteria from infected C. batrachus was found variation of number in different organs. Total bacterial load was found to be 1.16 x 105 to 3.15 x 106 cfu/g in lesions, 2.14 x 108 to 4.17 x 109 cfu/g in liver, 1.90 x 107 to 5.12 x 108 cfu/g in spleen and 2.32 x 106 to 5.24 x 108 cfu/g in kidney of infected C. batrachus in Mymensingh district. The isolates were found to produce acid from arabinose, whereas acid and gas from different sugar media such as maltose, sucrose, and dextrose. Morover, they were capable to ferment glucose but resistant to vibriostatic agent 0129 test. Further identification of A. hydrophila was accomplished using PCR. The PCR products of desired 760 bp were obtained for A. hydrophila. The isolated A. hydrophila were 96% sensitive to Enrofloxacin followed by 88% to Ciprofloxacin and 76% to Levofloxacin. On the other way, 100% were resistant to the Ampicillin followed by 96% to Penicillin and 92% to Novobiocin. So far, this is the first molecular identification of A. hydrophila from farmed C. batrachus in Bangladesh. The present study will provide future research scopes on identification of pathogenicity island in chromosome and serotyping of all A. hydrophila isolates. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 17-24
本研究于2014年11月至2015年2月在Mymensingh地区进行,目的是从受感染的呼吸鲶鱼Magur (Clarias batrachus)中分离出重要的鱼类病原体嗜水气单胞菌。从感染的棘球蚴中分离出的细菌进行定量研究,发现其在不同器官中的数量存在差异。Mymensingh地区感染batrachus的总细菌负荷为病变1.16 × 105 ~ 3.15 × 106 cfu/g,肝脏2.14 × 108 ~ 4.17 × 109 cfu/g,脾脏1.90 × 107 ~ 5.12 × 108 cfu/g,肾脏2.32 × 106 ~ 5.24 × 108 cfu/g。该菌株从阿拉伯糖中产生酸,而从不同的糖介质如麦芽糖、蔗糖和葡萄糖中产生酸和气体。对葡萄糖有发酵能力,对抑振剂0129有抗性。利用PCR技术对嗜水单胞菌进行进一步鉴定。结果表明,该菌株的PCR产物长度为760 bp。分离的嗜水单胞菌对恩诺沙星的敏感性为96%,对环丙沙星的敏感性为88%,对左氧氟沙星的敏感性为76%。氨苄西林耐药率为100%,其次为青霉素耐药率96%,新霉素耐药率92%。到目前为止,这是首次从孟加拉国养殖的batrachus中鉴定出嗜水单胞杆菌。本研究将为今后嗜水单胞菌的染色体致病性岛鉴定和血清分型提供研究方向。亚洲Australas。J.食品安全。安全,2017,1 (1),17-24
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引用次数: 3
Microbial assessment of milk collected from different markets of Mymensingh, Gazipur and Sherpur districts of Bangladesh and determination of antimicrobial resistance patterns of the isolated bacteria 对从孟加拉国Mymensingh、Gazipur和Sherpur地区不同市场收集的牛奶进行微生物评估,并确定分离细菌的抗微生物药物耐药性模式
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v1i1.55756
M. F. Hossain, M. Rahman, S. Kabir
The study was conducted to determine the total viable count (TVC) and total coliform count (TCC) of unpasteurized, pasteurized and UHT milk samples to assess their microbiological quality. A total of 60 milk samples were collected from different markets of Mymensingh, Gazipur and Sherpur districts. The TVC of milk was performed to determine the bacterial load in supplied milk samples and TCC to determine the coliform bacterial load in collected milk samples. Milk samples were cultured onto various selective media for the isolation of bacteria. The isolated bacteria were identified by cultural properties on different selective media, biochemical tests and finally by PCR. Out of 60 samples 20 unpasteurized milk samples from different markets were found positive for Staphylococcus aureus and 19 unpasteurized milk samples were found positive for Escherichia coli. All the S. aureus were found positive for S. aureus specific 16S rRNA gene by PCR. Out of 19 isolates of E. coli, 15 were found positive for E. coli 16S rRNA gene by PCR. Results of antimicrobial susceptibility test showed that most of the isolates of S. aureus and E .coli were susceptible to azithromycin, streptomycin, gentamicin, norfloxacin, tetracycline and ciprofloxacin but resistant to amoxicillin and erythromycin. The findings of this study revealed the presence of multidrug resistant S. aureus and E. coli in unpasteurized milk samples that posseses a serious threat to public health. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 7-16
本研究测定了未经巴氏杀菌、巴氏杀菌和超高温灭菌的牛奶样品的总活菌计数(TVC)和总大肠菌群计数(TCC),以评价其微生物质量。从Mymensingh、Gazipur和Sherpur地区的不同市场共收集了60份牛奶样本。采用牛奶TVC法测定供应牛奶样品中的细菌负荷,TCC法测定收集牛奶样品中的大肠菌群负荷。牛奶样品在不同的培养基上培养以分离细菌。通过不同培养基的培养特性、生化试验和PCR鉴定分离的细菌。在60个样本中,20个来自不同市场的未经巴氏消毒的牛奶样本被发现对金黄色葡萄球菌呈阳性反应,19个未经巴氏消毒的牛奶样本被发现对大肠杆菌呈阳性反应。所有金黄色葡萄球菌经PCR均检测出金黄色葡萄球菌特异性16S rRNA基因阳性。在19株大肠杆菌中,PCR检测出15株大肠杆菌16S rRNA基因阳性。药敏试验结果显示,大部分金黄色葡萄球菌和大肠杆菌对阿奇霉素、链霉素、庆大霉素、诺氟沙星、四环素和环丙沙星敏感,对阿莫西林和红霉素耐药。本研究结果表明,未经巴氏消毒的牛奶样品中存在耐多药金黄色葡萄球菌和大肠杆菌,对公众健康构成严重威胁。亚洲Australas。J.食品安全。安全,2017,1 (1),7-16
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引用次数: 4
Assessment of nutritional status and other associated factors of lactating mother at Gangni Pauroshava in Meherpur district, Bangladesh 孟加拉国Meherpur地区Gangni Pauroshava哺乳期母亲营养状况及其他相关因素评估
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v1i1.55759
Md. Tanvir Islam, Md Ashrafuzzaman Zahid, Md. Ismail Hossain, Rashida Parvin
The present study was to provide information about the nutritional status of lactating women as well as proffer solutions that are aimed at improving maternal nutrition in the study area. The study area was Gangni union of Meherpur district. 105 exclusively breastfeeding women aged 16-35 years old were initially enrolled in the study. Of these study lactating women completed 24h dietary recall food intake. The findings was about 16.2% lactating mothers were under 19 years, 53.3% respondents were between the ages of 20-25 years, 23.8% respondents were between the ages of 26-30 years and 37.1% lactating mothers had completed primary education. About 95.2% respondents were housewife and 4.8% respondents were service holders. The finding was 56.2% respondents have one child and 38.1% respondents have 3 family members, 43.8% respondent has 4 family members. About 7.6% respondents are underweight, 55.2% respondents are normal, 33.3% respondents are overweight and 3.8% respondents are obese. Total 7.6% respondents are underweight, 55.2% respondents are normal, 33.3% respondents are overweight and 3.8% respondents are obese where 5.7% respondents under 19 year aged and 1.9% respondents over 30 year aged are underweight. About 40% respondents got surgerian delivery where 60% respondents got normal delivery. It was found that 100% respondents maintain hygiene such as washing hands before eating foods, washing hands after using bathroom, brushing teeth regularly, avoiding nasty foods etc. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 35-44
本研究旨在提供有关哺乳期妇女营养状况的信息,并提供旨在改善研究地区产妇营养的解决办法。研究区为梅尔布尔县甘尼联合。105名年龄在16-35岁之间的纯母乳喂养女性最初参加了这项研究。在这些研究中,哺乳期妇女完成了24小时的饮食回忆。调查结果显示,年龄在19岁以下的哺乳母亲占16.2%,年龄在20-25岁之间的占53.3%,年龄在26-30岁之间的占23.8%,受过小学教育的哺乳母亲占37.1%。95.2%的受访者为家庭主妇,4.8%为服务业从业人员。调查结果显示,56.2%的受访者有一个孩子,38.1%的受访者有3个家庭成员,43.8%的受访者有4个家庭成员。7.6%的人体重过轻,55.2%的人正常,33.3%的人超重,3.8%的人肥胖。体重过轻者占7.6%,正常者占55.2%,超重者占33.3%,肥胖者占3.8%,其中19岁以下者体重过轻者占5.7%,30岁以上者体重过轻者占1.9%。约40%的受访者选择手术分娩,60%的受访者选择正常分娩。调查发现,100%的受访者保持卫生,如吃饭前洗手、上厕所后洗手、经常刷牙、避免食用讨厌的食物等。亚洲Australas。J.食品安全。安全,2017,1 (1),35-44
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引用次数: 0
Effects of gamma irradiation on shelf life and quality of Black Bengal goat meat 辐照对黑孟加拉山羊肉保质期和品质的影响
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v1i1.55763
Saddam Hossain Ruman Khan, M. Salahuddin, Md. Rezwanul Habib, Mostain Billah, Md Abu Haris Miah, S. Akhter, M. A. Hashem
This study was undertaken to assess the effects of gamma irradiation (0, 1.5, 2, 4 kGy) on shelf life and meat quality of Black Bengal goat. About 3 kg of fresh Black Bengal goat meat samples were taken and divided into four groups like T1 (non-irradiated, control), T2 (irradiated, 1.5 KGy), T3 (irradiated, 2.0 KGy) and T4 (irradiated, 4.0 KGy). Irradiated and non-irradiated meat samples were stored at refrigeration temperature to elucidate the storage effect. One way ANOVA was performed to investigate the effects of gamma irradiation on different groups. Significant differences were found in color and overall acceptability compared to non-irradiated group and only overall acceptability significantly decreased with increasing storage. No significant differences were observed in flavor, tenderness and juiciness between non-irradiated and irradiated groups. Irradiation influenced color and overall acceptability of chevon. Dry matter content was found higher in 4 KGy irradiated group and increased gradually in day intervals. Cooking loss, free fatty acid, peroxide value and thiobarbituric acid-reactive substances indicated 1.5 KGy irradiation rated best. Microbial findings revealed that 2 KGy irradiated group is better due to safe level of microbial loads which increase shelf life of Black Bengal goat meat. Finally, it may be concluded that 1.5 and 2 KGy doses gamma irradiation in Bengal goat meat enhances sensory attributes, physico-chemical and microbial levels found satisfactory. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 65-73
本研究旨在评估γ辐照(0、1.5、2、4 kGy)对黑孟加拉山羊保质期和肉质的影响。选取新鲜黑孟加拉山羊肉样品约3 kg,分为T1(未辐照对照)、T2(辐照1.5 KGy)、T3(辐照2.0 KGy)和T4(辐照4.0 KGy) 4组。辐照和未辐照的肉类样品在冷藏温度下保存,以阐明储存效果。采用单因素方差分析(One - way ANOVA)研究辐照对不同组的影响。与未辐照组相比,在颜色和总体可接受性方面存在显著差异,只有总体可接受性随着储存的增加而显著降低。未辐照组和辐照组在风味、嫩度和多汁性方面无显著差异。辐照影响颜色和整体可接受度。干物质含量在4 KGy辐照组较高,且随时间间隔逐渐升高。蒸煮损失、游离脂肪酸、过氧化值和硫代巴比妥酸活性物质表明1.5 KGy辐照效果最佳。微生物试验结果表明,2 KGy辐照组的微生物负荷安全水平较好,延长了黑孟加拉山羊肉的保质期。最后,可以得出结论,1.5和2 KGy剂量的伽玛辐照可以提高孟加拉山羊肉的感官属性、理化和微生物水平。亚洲Australas。J.食品安全。安全,2017,1 (1),65-73
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引用次数: 2
Effect of dietary probiotics on the growth performance, meat quality improvement of broiler chicken for safe meat production 饲粮中添加益生菌对肉鸡生长性能和肉品质的影响,促进肉品安全生产
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v1i1.55761
Md. Sazedul Karim Sarker, M. Rana, S. Sultana, N. R. Sarker, T. N. Nahar
A feeding trial was conducted to investigate the effect of Probiotic (Biofast) on the growth performance and meat quality of broiler chicken. Ninety six 1-d-old mix sexed Cobb 500 broiler chicks were obtained from a local commercial hatchery. Chicks were randomly allocated in 2 experimental treatments for 5 wk. The experimental treatments received a 1) control, 2) control with probiotic Biofast 0.055% (bacillus subtilis-100%) in the diet. Both treatments had 48 broilers arranged in 4 replicates of 12 broilers each. Eight birds from 2 different groups (one/replication) were sacrificed on termination of the feeding trial of breast, thigh to investigate the meat quality. Feed intake in treatment T1 was significantly (P > 0.05) greater than control in 0 to 3 weeks of age. Though in starter phase treatments failed to induce any marked effects on body weight, weight gain and FCR but numerically increased in T1 than control. In finisher period (4-5 wks), there were no significant differences on body weight, weight gain, feed intake and FCR between the treatments. The organ weight like liver, heart, kidney, spleen, gizzard, abdominal fat and intestine weight also did not show any significant differences between the dietary treatments of control and Biofast. In addition, though the dressing % was not significantly different but numerically higher percentage was found by using Biofast. Similarly, no significant differences (p>0.05) were found in pH, cooking loss, meat color and TBA values between the treatments. In conclusion, supplementation of Biofast in diet has no significant effect on the growth performance of broiler chicks though it has got some positive effects on other parameters that indirectly revealed to enhance meat quality of broiler chicken as well as food safety issues. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 51-57
本试验旨在研究益生菌(Biofast)对肉鸡生长性能和肉品质的影响。从当地一家商业孵化场获得96只1龄混合性科布500肉鸡。雏鸡随机分为2个试验处理,试验期5周。试验组设1)对照组,2)饲粮中添加0.055%(枯草芽孢杆菌-100%)益生菌的对照组。2个处理48只肉鸡,随机分成4个重复,每个重复12只。选取2个不同组(1个重复),在胸、大腿饲养试验结束时处死8只,考察肉质。0 ~ 3周龄,处理T1采食量显著(P > 0.05)高于对照组。虽然在起始阶段,处理对体重、增重和FCR没有显著影响,但在T1阶段,处理对体重、增重和FCR的影响明显高于对照组。在育肥期(4 ~ 5周),各处理的体重、增重、采食量和饲料转化率均无显著差异。肝脏、心脏、肾脏、脾脏、砂囊、腹部脂肪和肠道重量在对照组和bifast组之间也没有显著差异。另外,施用Biofast后,处理率虽无显著差异,但数值上较高。pH、蒸煮损失、肉色和TBA值在不同处理间无显著差异(p>0.05)。综上所述,饲粮中添加bifast对肉鸡生长性能无显著影响,但对其他参数有一定的积极影响,间接揭示了提高肉鸡肉品质和食品安全问题。亚洲Australas。J.食品安全。安全,2017,1 (1),51-57
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引用次数: 1
Bacteriological quality assessment of buffalo meat collected from different districts of Bangladesh with particular emphasis on the molecular detection and antimicrobial resistance of the isolated Salmonella species 对孟加拉国不同地区收集的水牛肉进行细菌学质量评估,特别强调分离沙门氏菌的分子检测和抗菌素耐药性
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v2i1.55895
M. Hasan, S. Kabir, M. Rahman, Y. A. Sarker
The research project was conducted to assess the bacteriological quality of buffalo meat samples collected from three upazilas namely Haluaghat, Sreepur and Madhupur of Bangladesh under the districts of Mymensingh, Gazipur and Tangail respectively with particular emphasis on the molecular detection and antimicrobial resistance of the isolate Salmonella species. Total viable count (TVC), total staphylococcal count (TStaC) and total salmonella count (TSC) of meat samples were determined and the mean values of TVC, TStaC and TSC for the Haluaghat, Sreepur and Madhupur were log 8.30, log 7.94, log 8.15; log6.21, log 6.40, log 5.43 and log 4.76, log 4.82, log 4.56 CFU/gm respectively which exceeded the ICMSF recommendations values. The variation of TVC and TSC in meats of different buffalo markets was significant at 5% level where the variation of TStaC was significant at 1% level. Nevertheless no significant variation was demonstrated between the interactions of the three upazilas. Among the samples, 46.67% (n=14) were found to be associated with Salmonella spp. The Salmonella spp. were identified by observing black centered colonies on XLD agar, positive to MR test and negative to VP and Indole test. All isolates of Salmonella spp. were positive to 16s rRNA gene based PCR (574bp). All isolates of Salmonella species were susceptible to ciprofloxacin, streptomycin and gentamicin. All isolates of Salmonella spp. (n=14; 100%) were resistant to amoxicillin and few isolates also resistant to erythromycin, tetracycline, azithromycin and cephradine. The findings of this study revealed the presence of multidrug resistant Salmonella spp. in buffalo meat of Mymensingh, Gazipur and Tangail districts of Bangladesh that posseses a serious threat to public health. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 12-20
本研究项目对分别采自孟加拉国Mymensingh、Gazipur和Tangail地区的Haluaghat、Sreepur和Madhupur三个地区的水牛肉样品进行细菌学质量评估,重点研究分离沙门氏菌的分子检测和耐药性。测定肉品总活菌数(TVC)、总葡萄球菌数(TStaC)和总沙门氏菌数(TSC), Haluaghat、Sreepur和Madhupur的TVC、TStaC和TSC的平均值分别为log 8.30、log 7.94和log 8.15;log6.21、log6.40、log5.43和log4.76、log4.82、log4.56 CFU/gm均超过ICMSF推荐值。不同水牛市场肉品中TVC和TSC在5%水平上差异显著,TStaC在1%水平上差异显著。然而,在三种upazilas的相互作用之间没有显着变化。其中46.67% (n=14)的样品与沙门氏菌有关,通过观察XLD琼脂上黑色中心菌落鉴定沙门氏菌,MR试验阳性,VP和吲哚试验阴性。所有分离株沙门氏菌的16s rRNA基因PCR检测均为阳性(574bp)。沙门氏菌对环丙沙星、链霉素和庆大霉素均敏感。所有沙门氏菌分离株(n=14;100%)对阿莫西林耐药,少数菌株对红霉素、四环素、阿奇霉素和头孢定耐药。这项研究的结果表明,孟加拉国Mymensingh、Gazipur和Tangail地区的水牛肉中存在耐多药沙门氏菌,对公众健康构成严重威胁。亚洲Australas。J.食品安全。安全,2018,2(1),12-20
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引用次数: 7
An assessment of socio-economic conditions of the farmers related to goat fattening in Rangpur district of Bangladesh 对孟加拉国Rangpur地区与山羊育肥有关的农民的社会经济条件的评估
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v1i1.55755
Topon Chandra Barman, M. M. Hossain, M. Rahman, Md Younus Ali, N. R. Sarker
The experiment was carried out at Kaunia upazila in Rangpur district to investigate the socio-economic conditions of the farmers, use of different hormones, feed additives and their effects. The data were collected through interview schedule by selecting 30 respondents who were involved in goat fattening. Collected data were analyzed using Excel sheet. It was found that most of the farmers were middle aged categories (50%) and educational level of the farmers was primary (57%), secondary (23%), and higher secondary (10%). Half of the respondents were farmer, followed by businessman (20%). About 67% farmers used own capital, 3% farmers took loan from bank and 23% took loan from other sources such as NGO. It was also found that about 33% respondents had on short time training experience and the rest 67% had no experience. Almost 97% farmers reared Black Bengal goat and 3% Jamunapari goat. Near about 97% farmers used roadside grass and tree leaves and only 3% farmers used cultivated fodder. Only 7% farmer used growth promoter & feed additives in feed where 93% farmers are not being used growth promoter &feed additives in feed. But not a single farmer used growth hormones for fattening purpose. Therefore, still now goat meat would be safe for human consumption without any health hazard. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 1-6
该试验在Rangpur地区的Kaunia upazila进行,目的是调查农民的社会经济条件、不同激素、饲料添加剂的使用及其效果。数据采用访谈法收集,选取30名参与山羊育肥的调查对象。收集的数据使用Excel表格进行分析。研究发现,农民以中年人居多(50%),受教育程度依次为小学(57%)、中学(23%)和高中(10%)。一半的受访者是农民,其次是商人(20%)。约67%的农民使用自有资金,3%的农民从银行贷款,23%的农民从非政府组织等其他渠道贷款。调查还发现,约33%的受访者没有短期培训经验,其余67%的受访者没有培训经验。几乎97%的农民饲养黑孟加拉山羊和3%的贾穆纳帕里山羊。近97%的农民使用路边的草和树叶,只有3%的农民使用人工培育的饲料。只有7%的农民在饲料中使用生长促进剂和饲料添加剂,而93%的农民在饲料中没有使用生长促进剂和饲料添加剂。但没有一个农民将生长激素用于增肥。因此,现在食用山羊肉仍然是安全的,没有任何健康危害。亚洲Australas。J.食品安全。安全,2017,1 (1),1-6
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引用次数: 9
Bacteriological assessment of tap water collected from different markets of Mymensingh, Gazipur and Sherpur districts of Bangladesh with special focus on the molecular detection and antimicrobial resistance of the isolated Escherichia coli 对从孟加拉国Mymensingh、Gazipur和Sherpur地区不同市场收集的自来水进行细菌学评估,特别侧重于分离的大肠杆菌的分子检测和抗菌素耐药性
Pub Date : 1900-01-01 DOI: 10.3329/aajfss.v2i1.55897
Md. Shihab Hassan, S. Kabir, Y. A. Sarker, M. Rahman
The objectives of this study were to assess the bacteriological quality of tap water samples obtained from different markets of different upazillas of Mymensingh, Sherpur & Gazipur district. For achieving the above mentioned objectives, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated E. coli from tap water samples were characterized by using biochemical test, molecular method and antimicrobial susceptibility tests. HPC was highest in market tap water collected from Kaligonj and TCC was highest in market tap water of collected from Mymensingh sadar. The geometric mean of HPC of Mymensingh, Gazipur and Sherpur districts water was 8.4x105, 2.5 x106 and 6.8 x105 C.F.U/100 ml. All isolates of E. coli (n=20) were amplified by using 16S rRNA gene based PCR. In respect to antimicrobial susceptibility testing, most of the E. coli isolates were susceptible to norfloxacin, ampicilin, tetracycline, streptomycin and ciprofloxacin. Furthermore, a few E. coli isolates were intermediate resistant to gentamycin and ciprofloxacin. However, a few of the E. coli isolates were resistant to erythromycin and amoxycilin. Moreover, out of 20 E. coli isolates 3 (15%) isolates were detected as multidrug resistant. This study indicated the presence of multidrug resistant E. coli isolates in tap water in Mymensingh, Sherpur and Gazipur districts that warrants particular attention. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 21-28
本研究的目的是评估从Mymensingh, Sherpur和Gazipur地区不同upazillas的不同市场获得的自来水样品的细菌学质量。为了达到上述目的,采用了异养平板计数(HPC)和总大肠菌群计数(TCC)的方法。采用生化试验、分子法和药敏试验对自来水中分离的大肠杆菌进行了鉴定。卡利贡吉市自来水中HPC含量最高,迈门辛格萨达尔市自来水中TCC含量最高。Mymensingh、Gazipur和Sherpur地区水体HPC的几何平均值分别为8.4 × 105、2.5 × 106和6.8 × 105 C.F.U/100 ml。所有分离株(n=20)均采用16S rRNA基因PCR扩增。在药敏试验中,大多数大肠杆菌对诺氟沙星、氨苄西林、四环素、链霉素和环丙沙星敏感。此外,少数大肠杆菌分离株对庆大霉素和环丙沙星具有中等耐药性。然而,少数大肠杆菌分离株对红霉素和阿莫西林耐药。此外,在20株大肠杆菌中,3株(15%)被检测出多重耐药。该研究表明,Mymensingh、Sherpur和Gazipur地区自来水中存在多重耐药大肠杆菌分离株,值得特别关注。亚洲Australas。J.食品安全。安全,2018,2(1),21-28
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引用次数: 7
期刊
Asian-Australasian Journal of Food Safety and Security
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