Pub Date : 2025-01-31DOI: 10.1016/j.bios.2025.117220
Qichang Hu , Minhui Hong , Zhao Wang , Xiuyu Lin , Wei Wang , Wei Zheng , Shungui Zhou
Developing the integration of self-powered detection with both dynamic and static forces is a significant challenge in promoting intelligent technology systems. Herein, we introduce an innovative microbial biofilm based-hydrovoltaic pressure sensor (mBio-HPS) using whole-cell Geobacter sulfurreducens, which successfully combines self-powered functionality and static pressure detection within a single device. The mBio-HPS exhibited a sensitivity of up to 8968.7 kPa⁻1 (at 1 kPa) in the 0.4–25 kPa regime without external power supply. Moreover, the mBio-HPS demonstrated the fastest reported response speed to date, with a remarkable response time of 112.5 μs, enabling effective detection of both dynamic and static forces while maintaining stability during an extensive 30,000 s testing. Experimental validation using a sensor-integrated array showed its outstanding real-time detection capabilities for both dynamic and static pressure, highlighting its outstanding potential for electronic skin applications. This unprecedented concept of a hydrovoltaic pressure sensor also offers new insights into the development of high-performance self-powered electronics.
{"title":"Microbial biofilm-based hydrovoltaic pressure sensor with ultrahigh sensitivity for self-powered flexible electronics","authors":"Qichang Hu , Minhui Hong , Zhao Wang , Xiuyu Lin , Wei Wang , Wei Zheng , Shungui Zhou","doi":"10.1016/j.bios.2025.117220","DOIUrl":"10.1016/j.bios.2025.117220","url":null,"abstract":"<div><div>Developing the integration of self-powered detection with both dynamic and static forces is a significant challenge in promoting intelligent technology systems. Herein, we introduce an innovative microbial biofilm based-hydrovoltaic pressure sensor (mBio-HPS) using whole-cell <em>Geobacter sulfurreducens</em>, which successfully combines self-powered functionality and static pressure detection within a single device. The mBio-HPS exhibited a sensitivity of up to 8968.7 kPa⁻<sup>1</sup> (at 1 kPa) in the 0.4–25 kPa regime without external power supply. Moreover, the mBio-HPS demonstrated the fastest reported response speed to date, with a remarkable response time of 112.5 μs, enabling effective detection of both dynamic and static forces while maintaining stability during an extensive 30,000 s testing. Experimental validation using a sensor-integrated array showed its outstanding real-time detection capabilities for both dynamic and static pressure, highlighting its outstanding potential for electronic skin applications. This unprecedented concept of a hydrovoltaic pressure sensor also offers new insights into the development of high-performance self-powered electronics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117220"},"PeriodicalIF":10.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-31DOI: 10.1016/j.bios.2025.117205
Xiankai Niu , Yiqun Guo , Na Li , Siyu Li , Yilei Yu , Jianwei Jiao , Yuna Guo
Exosomal RNA has emerged as a promising biomarker for glioblastoma (GBM) due to its exceptional stability in biofluids and strong correlation with tumor progression. In this study, we present an innovative intelligent molecular cleavage and dual-signal relay amplification-based ratiometric (ISR) strategy for high-sensitivity monitoring and dynamic analysis of exosomal RNA in glioma. The core mechanism is based on a hollow duplex structure that effectively prevents premature cleavage by duplex-specific nuclease (DSN), ensuring both the accuracy and stability of the detection system. Upon the introduction of the target microRNA (miRNA), one strand of the hollow duplex is displaced, forming a miRNA-DNA duplex that serves as a substrate for DSN, initiating target recycling and signal amplification. This dynamic process, coupled with dual-signal relay amplification, significantly enhances both sensitivity and stability, even at low miRNA concentrations. Our ratiometric approach substantially improves detection accuracy by comparing dual signal outputs. We further demonstrate the capability of real-time tracking of exosomal RNA dynamics, enabling precise monitoring of miRNA fluctuations over time. The practical applicability of our ISR strategy was validated by accurately detecting exosomal miRNA levels in clinical serum samples from glioblastoma patients, distinguishing them from healthy controls with high precision. Our method represents a significant advancement in early cancer detection and disease monitoring, with broad implications for precision medicine and the development of point-of-care diagnostic tools. Looking ahead, the ISR strategy holds great potential for monitoring a wide range of diseases, offering new opportunities for personalized diagnostics and therapeutic strategies.
{"title":"Intelligent molecular cleavage and dual-signal relay amplification ratiometric strategy for high-sensitivity analysis and dynamic monitoring of exosomal RNA in glioma","authors":"Xiankai Niu , Yiqun Guo , Na Li , Siyu Li , Yilei Yu , Jianwei Jiao , Yuna Guo","doi":"10.1016/j.bios.2025.117205","DOIUrl":"10.1016/j.bios.2025.117205","url":null,"abstract":"<div><div>Exosomal RNA has emerged as a promising biomarker for glioblastoma (GBM) due to its exceptional stability in biofluids and strong correlation with tumor progression. In this study, we present an innovative intelligent molecular cleavage and dual-signal relay amplification-based ratiometric (ISR) strategy for high-sensitivity monitoring and dynamic analysis of exosomal RNA in glioma. The core mechanism is based on a hollow duplex structure that effectively prevents premature cleavage by duplex-specific nuclease (DSN), ensuring both the accuracy and stability of the detection system. Upon the introduction of the target microRNA (miRNA), one strand of the hollow duplex is displaced, forming a miRNA-DNA duplex that serves as a substrate for DSN, initiating target recycling and signal amplification. This dynamic process, coupled with dual-signal relay amplification, significantly enhances both sensitivity and stability, even at low miRNA concentrations. Our ratiometric approach substantially improves detection accuracy by comparing dual signal outputs. We further demonstrate the capability of real-time tracking of exosomal RNA dynamics, enabling precise monitoring of miRNA fluctuations over time. The practical applicability of our ISR strategy was validated by accurately detecting exosomal miRNA levels in clinical serum samples from glioblastoma patients, distinguishing them from healthy controls with high precision. Our method represents a significant advancement in early cancer detection and disease monitoring, with broad implications for precision medicine and the development of point-of-care diagnostic tools. Looking ahead, the ISR strategy holds great potential for monitoring a wide range of diseases, offering new opportunities for personalized diagnostics and therapeutic strategies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"274 ","pages":"Article 117205"},"PeriodicalIF":10.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.bios.2025.117219
André Olean-Oliveira , Patrícia Monteiro Seraphim , Miquéias L. Portugal , Marcos F.S. Teixeira
Detection of glucose transporter 4 (GLUT4) is essential for understanding various physiological and pathological processes. This work reports the development of a novel electrochemical immunosensor for the direct detection of GLUT4, employing dissolved oxygen as a redox probe. This molecular oxygen-sensitive response is mediated by a redox-conductive polymer based on thionine. The sensor platform was fabricated via a one-step electropolymerization of thionine and gold nanoparticles (AuNPs) onto a platinum screen-printed electrode (Olean-Oliveira et al., 2022a). The immunosensor was then constructed by physical adsorption of a GLUT4 antibody onto the poly(thionine)-AuNP composite surface. This label-free approach eliminates the need for secondary antibodies or enzymes. The immunosensor performance was evaluated using electrochemical impedance spectroscopy (EIS). The sensing mechanism relies on impedance changes; increasing GLUT4 concentrations lead to increased impedance due to enhanced surface blocking upon GLUT4-antibody binding. This interaction impedes oxygen diffusion to the polymer redox sites, resulting in increased electrical resistance. Analysis of the redox capacitance as a function of frequency demonstrates a decrease in the capacitive arc with increasing GLUT4 concentration.
{"title":"A novel GLUT-4 electrochemical immunosensor based on a poly(thionine)-gold nanoparticle nanocomposite: Combining complex capacitance and dissolved oxygen to obtain an analytical signal","authors":"André Olean-Oliveira , Patrícia Monteiro Seraphim , Miquéias L. Portugal , Marcos F.S. Teixeira","doi":"10.1016/j.bios.2025.117219","DOIUrl":"10.1016/j.bios.2025.117219","url":null,"abstract":"<div><div>Detection of glucose transporter 4 (GLUT4) is essential for understanding various physiological and pathological processes. This work reports the development of a novel electrochemical immunosensor for the direct detection of GLUT4, employing dissolved oxygen as a redox probe. This molecular oxygen-sensitive response is mediated by a redox-conductive polymer based on thionine. The sensor platform was fabricated via a one-step electropolymerization of thionine and gold nanoparticles (AuNPs) onto a platinum screen-printed electrode (Olean-Oliveira et al., 2022a). The immunosensor was then constructed by physical adsorption of a GLUT4 antibody onto the poly(thionine)-AuNP composite surface. This label-free approach eliminates the need for secondary antibodies or enzymes. The immunosensor performance was evaluated using electrochemical impedance spectroscopy (EIS). The sensing mechanism relies on impedance changes; increasing GLUT4 concentrations lead to increased impedance due to enhanced surface blocking upon GLUT4-antibody binding. This interaction impedes oxygen diffusion to the polymer redox sites, resulting in increased electrical resistance. Analysis of the redox capacitance as a function of frequency demonstrates a decrease in the capacitive arc with increasing GLUT4 concentration.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117219"},"PeriodicalIF":10.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143264185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.bios.2025.117218
Adel Yavarinasab , Jerry He , Abhirup Mookherjee , Nikhil Krishnan , Luis Ruiz Pestana , Diana Fusco , Dan Bizzotto , Carolina Tropini
Biofilms are structured microbial communities, known for their electron transfer properties, which are essential for metabolic processes and microbial survival. Here, we investigated the electrogenic properties of Bacillus subtilis, a bacterial producer of electron-donating biofilms. Interdigitated gold electrodes were utilized to continuously measure the electrochemical activity of biofilm-forming B. subtilis cells and genetic mutants unable to create them (biofilm-deficient). The formation of extracellular polymeric substances (EPS) and filamentous appendages was monitored via scanning electron microscopy (SEM). Chronoamperometry was used to assess electrochemical activity, which showed fluctuations in electrical current at specific time points in biofilm-forming cells. Cyclic voltammetry (CV) revealed significant differences between the voltammograms of biofilm-forming and biofilm-deficient cells, hypothesized to be a result of the reduction of secreted flavodoxin. Electrochemical impedance spectroscopy (EIS) was also performed at various intervals and analyzed using an equivalent circuit. We identified the presence of a charge transfer resistance (Rct) exclusively in biofilm which correlated to the time of increased electrochemical activity measured using chronoamperometry. Finally, through confocal microscopy, we found that the expression of a gene involved in biofilm matrix formation, tasA, was correlated with the time when charge transfer was measured. These results indicate that electrochemical activity is primarily present in biofilm-forming cells rather than in biofilm-deficient mutants. By combining electrochemical and microscopic methods, we developed a methodology to continuously monitor the stages of biofilm formation and showed that electrochemical activities within biofilms vary over time and there is a temporal relationship between these processes and the expression of genes responsible for biofilm development.
{"title":"Electrogenic dynamics of biofilm formation: Correlation between genetic expression and electrochemical activity in Bacillus subtilis","authors":"Adel Yavarinasab , Jerry He , Abhirup Mookherjee , Nikhil Krishnan , Luis Ruiz Pestana , Diana Fusco , Dan Bizzotto , Carolina Tropini","doi":"10.1016/j.bios.2025.117218","DOIUrl":"10.1016/j.bios.2025.117218","url":null,"abstract":"<div><div>Biofilms are structured microbial communities, known for their electron transfer properties, which are essential for metabolic processes and microbial survival. Here, we investigated the electrogenic properties of <em>Bacillus subtilis</em>, a bacterial producer of electron-donating biofilms. Interdigitated gold electrodes were utilized to continuously measure the electrochemical activity of biofilm-forming <em>B. subtilis</em> cells and genetic mutants unable to create them (biofilm-deficient). The formation of extracellular polymeric substances (EPS) and filamentous appendages was monitored via scanning electron microscopy (SEM). Chronoamperometry was used to assess electrochemical activity, which showed fluctuations in electrical current at specific time points in biofilm-forming cells. Cyclic voltammetry (CV) revealed significant differences between the voltammograms of biofilm-forming and biofilm-deficient cells, hypothesized to be a result of the reduction of secreted flavodoxin. Electrochemical impedance spectroscopy (EIS) was also performed at various intervals and analyzed using an equivalent circuit. We identified the presence of a charge transfer resistance (R<sub>ct</sub>) exclusively in biofilm which correlated to the time of increased electrochemical activity measured using chronoamperometry. Finally, through confocal microscopy, we found that the expression of a gene involved in biofilm matrix formation, <em>tasA</em>, was correlated with the time when charge transfer was measured. These results indicate that electrochemical activity is primarily present in biofilm-forming cells rather than in biofilm-deficient mutants. By combining electrochemical and microscopic methods, we developed a methodology to continuously monitor the stages of biofilm formation and showed that electrochemical activities within biofilms vary over time and there is a temporal relationship between these processes and the expression of genes responsible for biofilm development.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"276 ","pages":"Article 117218"},"PeriodicalIF":10.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.bios.2025.117192
Xiaoting Niu , Yufan Fan , Guanghao Zhu , Hairong Zeng , Bei Zhao , Mengru Sun , Lin Chen , Luling Wu , Zhenhao Tian , Tony D. James , Guangbo Ge
Human estrogen sulfotransferase (hSULT1E1), an important conjugative enzyme, plays crucial roles in both estrogen homeostasis and xenobiotic metabolism. Herein, a rational substrate engineering strategy was adopted to construct highly specific fluorogenic substrates for hSULT1E1. In the 1st round of structure-based virtual screening, 4-hydroxyl-1,8-naphthalimide (4-HN) was identified as a suitable scaffold for constructing hSULT1E1 substrates. Subsequently, structural modifications on the north part of 4-HN generated a panel of derivatives as substrate candidates, in which HN-299 was identified as a highly selective fluorogenic substrate for hSULT1E1. In the 3rd round of structural optimization, a “molecular growth” strategy on the south part of HN-299 was used to develop a highly selective and reactive substrate (HN-375). Under physiological conditions, HN-375 could be readily sulfated by hSULT1E1 to generate a single fluorescent product, which emitted bright green signals at around 510 nm and was fully identified as HN-375 4-O-sulfate (HNS). Further investigations indicated that HN-375 exhibited excellent isoform-specificity, rapid-response, ultrahigh sensitivity, and high signal-to-noise ratio, and as such was subsequently used for sensing SULT1E1 activity in hepatocellular carcinoma specimens and live organs. With HN-375 in hand, a practical fluorescence-based assay was established for high-throughput screening and characterization of hSULT1E1 inhibitors, as such two potent hSULT1E1 inhibitors were identified from in-house compound libraries. Collectively, this study showcases a groundbreaking strategy for engineering highly specific and sensitive fluorogenic substrates for target conjugative enzyme(s), while HN-375 emerges as a practical tool for sensing SULT1E1 activity in a biological context and for the high-throughput screening of inhibitors.
{"title":"Rational engineering of isoform-specific hSULT1E1 fluorogenic substrates for functional analysis and inhibitor screening","authors":"Xiaoting Niu , Yufan Fan , Guanghao Zhu , Hairong Zeng , Bei Zhao , Mengru Sun , Lin Chen , Luling Wu , Zhenhao Tian , Tony D. James , Guangbo Ge","doi":"10.1016/j.bios.2025.117192","DOIUrl":"10.1016/j.bios.2025.117192","url":null,"abstract":"<div><div>Human estrogen sulfotransferase (hSULT1E1), an important conjugative enzyme, plays crucial roles in both estrogen homeostasis and xenobiotic metabolism. Herein, a rational substrate engineering strategy was adopted to construct highly specific fluorogenic substrates for hSULT1E1. In the 1<sup>st</sup> round of structure-based virtual screening, 4-hydroxyl-1,8-naphthalimide (<strong>4-HN</strong>) was identified as a suitable scaffold for constructing hSULT1E1 substrates. Subsequently, structural modifications on the north part of <strong>4-HN</strong> generated a panel of derivatives as substrate candidates, in which <strong>HN-299</strong> was identified as a highly selective fluorogenic substrate for hSULT1E1. In the 3<sup>rd</sup> round of structural optimization, a “molecular growth” strategy on the south part of <strong>HN-299</strong> was used to develop a highly selective and reactive substrate (<strong>HN-375</strong>). Under physiological conditions, <strong>HN-375</strong> could be readily sulfated by hSULT1E1 to generate a single fluorescent product, which emitted bright green signals at around 510 nm and was fully identified as <strong>HN-375</strong> 4-<em>O</em>-sulfate (<strong>HNS</strong>). Further investigations indicated that <strong>HN-375</strong> exhibited excellent isoform-specificity, rapid-response, ultrahigh sensitivity, and high signal-to-noise ratio, and as such was subsequently used for sensing SULT1E1 activity in hepatocellular carcinoma specimens and live organs. With <strong>HN-375</strong> in hand, a practical fluorescence-based assay was established for high-throughput screening and characterization of hSULT1E1 inhibitors, as such two potent hSULT1E1 inhibitors were identified from in-house compound libraries. Collectively, this study showcases a groundbreaking strategy for engineering highly specific and sensitive fluorogenic substrates for target conjugative enzyme(s), while <strong>HN-375</strong> emerges as a practical tool for sensing SULT1E1 activity in a biological context and for the high-throughput screening of inhibitors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117192"},"PeriodicalIF":10.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.bios.2025.117191
Seunghyeb Ban , Haran Lee , Jiehao Chen , Hee-Seok Kim , Yuhang Hu , Seong J. Cho , Woon-Hong Yeo
{"title":"Corrigendum to “Recent advances in implantable sensors and electronics using printable materials for advanced healthcare” [Biosens. Bioelectron. 257 (2024) 116302]","authors":"Seunghyeb Ban , Haran Lee , Jiehao Chen , Hee-Seok Kim , Yuhang Hu , Seong J. Cho , Woon-Hong Yeo","doi":"10.1016/j.bios.2025.117191","DOIUrl":"10.1016/j.bios.2025.117191","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117191"},"PeriodicalIF":10.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-28DOI: 10.1016/j.bios.2025.117203
Weijing Liu , Yue Wang , Wu Peng , Xianghu Zeng , Pengjun Jiang , Wei Xiao , Jie Chen , Piaopiao Chen
MicroRNAs (miRNAs) are critical regulators of gene expression, with aberrant levels linked to diseases such as breast cancer. Notably, they are challenging to detect due to their low abundance in complex sample matrices. In this study, a Cu2⁺-encapsulated DNA nanosphere system capable of homogeneous, enzyme-free, one-pot detection of microRNA-155 (miRNA-155) in human plasma was developed. The system employed self-assembled DNA nanospheres loaded with copper ions as specific recognizers, coupled with a filter membrane-assisted reaction to separate free-Cu2+ from residual components. Moreover, dual quantum dots (QDs) were utilized for signal output, using competitive binding to enhance sensitivity for detecting ultra-low and subtle changes in miRNA levels in complex samples. The method achieved an excellent detection performance, with a limit of detection (LOD) at the low-aM level. Additionally, it demonstrated high specificity in distinguishing between different miRNAs and single nucleotide polymorphisms (SNPs). Validation using 38 clinical plasma samples achieved over 95% accuracy in identifying breast cancer patients, demonstrating 100% sensitivity and approximately 90% clinical consistency compared to imaging, pathology, and quantitative real-time polymerase chain reaction (qRT-PCR). The method required minimal sample pre-treatment and was completed within 1 h. Overall, the developed method offers a reliable tool for breast cancer diagnosis and establishes a mode for extending its application to other biomarkers in the clinical setting.
{"title":"Cu2+-encapsulated DNA nanosphere and filter enable sensitive and rapid analysis of miRNA-155","authors":"Weijing Liu , Yue Wang , Wu Peng , Xianghu Zeng , Pengjun Jiang , Wei Xiao , Jie Chen , Piaopiao Chen","doi":"10.1016/j.bios.2025.117203","DOIUrl":"10.1016/j.bios.2025.117203","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are critical regulators of gene expression, with aberrant levels linked to diseases such as breast cancer. Notably, they are challenging to detect due to their low abundance in complex sample matrices. In this study, a Cu<sup>2</sup><sup>⁺</sup>-encapsulated DNA nanosphere system capable of homogeneous, enzyme-free, one-pot detection of microRNA-155 (miRNA-155) in human plasma was developed. The system employed self-assembled DNA nanospheres loaded with copper ions as specific recognizers, coupled with a filter membrane-assisted reaction to separate free-Cu<sup>2+</sup> from residual components. Moreover, dual quantum dots (QDs) were utilized for signal output, using competitive binding to enhance sensitivity for detecting ultra-low and subtle changes in miRNA levels in complex samples. The method achieved an excellent detection performance, with a limit of detection (LOD) at the low-aM level. Additionally, it demonstrated high specificity in distinguishing between different miRNAs and single nucleotide polymorphisms (SNPs). Validation using 38 clinical plasma samples achieved over 95% accuracy in identifying breast cancer patients, demonstrating 100% sensitivity and approximately 90% clinical consistency compared to imaging, pathology, and quantitative real-time polymerase chain reaction (qRT-PCR). The method required minimal sample pre-treatment and was completed within 1 h. Overall, the developed method offers a reliable tool for breast cancer diagnosis and establishes a mode for extending its application to other biomarkers in the clinical setting.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"274 ","pages":"Article 117203"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-28DOI: 10.1016/j.bios.2025.117217
Katarzyna Tyszczuk-Rotko , Katarzyna Staniec , Agnieszka Hanaka
A green, cost-effective, and efficient square-wave voltammetric (SWV) assay based on an electrochemically activated glassy carbon electrode (aGCE) for the determination of the plant growth regulator methyl jasmonate (MeJA) was developed. The activation was performed by anodization in 0.1 mol L−1 NaOH by 5 cyclic voltammetric measurements in the potential range of 0–2 V at a scan rate of 100 mV s−1. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) were applied to analyze the difference between the bare GCE and the aGCE in terms of their electrochemical properties. The functionalization of the GCE surface by oxygen-containing groups not only creates new active sites but also improves electron transfer dynamics and electrocatalytic activity. The SWV procedure displays a wide linear response from 0.1 to 50.0 μmol L−1, a low LOD = 0.027 μmol L−1, and LOQ = 0.097 μmol L−1. The aGCE was successfully applied to MeJA analysis in Phaseolus coccineus leaf extracts.
{"title":"Green and cost-effective voltammetric assay based on activated glassy carbon electrode for determination of the plant growth regulator methyl jasmonate","authors":"Katarzyna Tyszczuk-Rotko , Katarzyna Staniec , Agnieszka Hanaka","doi":"10.1016/j.bios.2025.117217","DOIUrl":"10.1016/j.bios.2025.117217","url":null,"abstract":"<div><div>A green, cost-effective, and efficient square-wave voltammetric (SWV) assay based on an electrochemically activated glassy carbon electrode (aGCE) for the determination of the plant growth regulator methyl jasmonate (MeJA) was developed. The activation was performed by anodization in 0.1 mol L<sup>−1</sup> NaOH by 5 cyclic voltammetric measurements in the potential range of 0–2 V at a scan rate of 100 mV s<sup>−1</sup>. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) were applied to analyze the difference between the bare GCE and the aGCE in terms of their electrochemical properties. The functionalization of the GCE surface by oxygen-containing groups not only creates new active sites but also improves electron transfer dynamics and electrocatalytic activity. The SWV procedure displays a wide linear response from 0.1 to 50.0 μmol L<sup>−1</sup>, a low LOD = 0.027 μmol L<sup>−1</sup>, and LOQ = 0.097 μmol L<sup>−1</sup>. The aGCE was successfully applied to MeJA analysis in <em>Phaseolus coccineus</em> leaf extracts.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"274 ","pages":"Article 117217"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-28DOI: 10.1016/j.bios.2025.117204
Rong Wang, Lingqi Kong, Mao Xia, Yaqin Chai, Ruo Yuan
Herein, a G-quadruplex-enriched DNA nanonetwork (GDN) self-assembled via Y-modules was designed to construct an ultrasensitive electrochemical biosensing platform with low background for the detection of mucin 1 related to cancers. The single-stranded DNA (ssDNA) S1 converted from target mucin 1 could hybridize with ssDNA S2 and ssDNA S3 with split G-quadruplex fragments at ends to form Y-modules and self-assemble into a GDN, which can capture abundant electroactive substance hemin for a significant electrochemical signal. Impressively, compared with conventional G-quadruplex nanowires with low loading capacity and poor structural stability, the GDN assembled by Y-modules was able to load more signaling probes and obtain a more stable structure for the support of G-quadruplexes, thereby outputting a stronger and more stable electrochemical signal. Moreover, a complete G-quadruplex assembled from two split G-quadruplex effectively reduced the background signal and thus improved the signal-to-noise ratio for the sensitive detection of mucin 1. As a result, the constructed electrochemical biosensor based on GDN achieved ultrasensitive detection of target mucin 1 with a detection limit down to 0.15 fg mL−1 and was successfully applied to analyze mucin 1 in human serum samples, exhibiting great potential for protein biomarker analysis and clinical diagnosis of diseases.
{"title":"An efficient signal amplification strategy with low background based on a G-quadruplex-enriched DNA nanonetwork for ultrasensitive electrochemical detection of mucin 1","authors":"Rong Wang, Lingqi Kong, Mao Xia, Yaqin Chai, Ruo Yuan","doi":"10.1016/j.bios.2025.117204","DOIUrl":"10.1016/j.bios.2025.117204","url":null,"abstract":"<div><div>Herein, a G-quadruplex-enriched DNA nanonetwork (GDN) self-assembled via Y-modules was designed to construct an ultrasensitive electrochemical biosensing platform with low background for the detection of mucin 1 related to cancers. The single-stranded DNA (ssDNA) S1 converted from target mucin 1 could hybridize with ssDNA S2 and ssDNA S3 with split G-quadruplex fragments at ends to form Y-modules and self-assemble into a GDN, which can capture abundant electroactive substance hemin for a significant electrochemical signal. Impressively, compared with conventional G-quadruplex nanowires with low loading capacity and poor structural stability, the GDN assembled by Y-modules was able to load more signaling probes and obtain a more stable structure for the support of G-quadruplexes, thereby outputting a stronger and more stable electrochemical signal. Moreover, a complete G-quadruplex assembled from two split G-quadruplex effectively reduced the background signal and thus improved the signal-to-noise ratio for the sensitive detection of mucin 1. As a result, the constructed electrochemical biosensor based on GDN achieved ultrasensitive detection of target mucin 1 with a detection limit down to 0.15 fg mL<sup>−1</sup> and was successfully applied to analyze mucin 1 in human serum samples, exhibiting great potential for protein biomarker analysis and clinical diagnosis of diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"275 ","pages":"Article 117204"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-28DOI: 10.1016/j.bios.2025.117212
Ya Zhang , Rouyu Su , Zheng Zhang , Yiyue Jiang , Yejia Miao , Shiqi Zhou , Miaomiao Ji , Chih-Wen Hsu , Hongpan Xu , Zhiyang Li , Guanghui Wang
Aberrant microRNA expression is associated with tumor progression in various organs. Detecting microRNAs as clinical cancer biomarkers can facilitate early cancer diagnosis and monitoring. However, the rapid and accurate quantification of microRNAs from biological samples remains a significant challenge. Here we developed a one-pot isothermal assay utilizing a molecular circuit with CRISPR/Cas13a (CRISPR-circuit) to rapidly convert, amplify and report different microRNAs within 15 min at the attomolar (aM) level. Then the full process was performed on an active centrifugal microfluidic chip and its corresponding portable equipment for parallel detection of multiple microRNAs, including miR-21, miR-141, miR-196a, and miR-1246. We also demonstrated its application for identifying cell lines and clinical samples of cancer patients with varying microRNA levels, which showed a strong correlation with the RT-qPCR. The assay can be easily adapted for the detection of any microRNA by simply modifying the converter primer, thereby holding significant potential for accurate disease detection and clinical diagnosis.
{"title":"An ultrasensitive one-pot Cas13a-based microfluidic assay for rapid multiplexed detection of microRNAs","authors":"Ya Zhang , Rouyu Su , Zheng Zhang , Yiyue Jiang , Yejia Miao , Shiqi Zhou , Miaomiao Ji , Chih-Wen Hsu , Hongpan Xu , Zhiyang Li , Guanghui Wang","doi":"10.1016/j.bios.2025.117212","DOIUrl":"10.1016/j.bios.2025.117212","url":null,"abstract":"<div><div>Aberrant microRNA expression is associated with tumor progression in various organs. Detecting microRNAs as clinical cancer biomarkers can facilitate early cancer diagnosis and monitoring. However, the rapid and accurate quantification of microRNAs from biological samples remains a significant challenge. Here we developed a one-pot isothermal assay utilizing a molecular circuit with CRISPR/Cas13a (CRISPR-circuit) to rapidly convert, amplify and report different microRNAs within 15 min at the attomolar (aM) level. Then the full process was performed on an active centrifugal microfluidic chip and its corresponding portable equipment for parallel detection of multiple microRNAs, including miR-21, miR-141, miR-196a, and miR-1246. We also demonstrated its application for identifying cell lines and clinical samples of cancer patients with varying microRNA levels, which showed a strong correlation with the RT-qPCR. The assay can be easily adapted for the detection of any microRNA by simply modifying the converter primer, thereby holding significant potential for accurate disease detection and clinical diagnosis.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"274 ","pages":"Article 117212"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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