Biosensors and Bioelectronics最新文献_第5页

A dual-quenching sensing strategy based on the quenching effect of Au/Ni-Co nanocages towards hydrophobic armor functionalized-Ru@HKUST-1/Pd for ultrasensitive detection of zearalenone 基于Au/Ni-Co纳米笼对疏水盔甲functionalized-Ru@HKUST-1/Pd猝灭效应的双猝灭传感策略用于超灵敏检测赤霉烯酮。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-21 DOI: 10.1016/j.bios.2025.118259

Jinxiu Zhao , Zuoxun Xie , Xue Dong , Huan Wang , Xiang Ren , Qin Wei

Zearalenone (ZEN) is predominantly generated by Fusarium graminearum. This mycotoxin is also inscribed in the carcinogen inventory issued by the International Agency for Research on Cancer of the World Health Organization. The primary target organ of ZEN is the reproductive system of female animals, though it also exerts specific impacts on male animals. Therefore, sensitive detection of this toxin is urgently needed. In this study, Ru(bpy)₃²⁺ and tri-n-propylamine (TPrA) were self-assembled into the pores of hydrophobic ionic liquid (HIL) functionalized HKUST-1, thereby preparing an ultra-sensitive electrochemiluminescence (ECL) microreactor to detect zearalenone (ZEN). In particular, the introduction of HIL changes the hydrophilic surface of HKUST-1 into a hydrophobic surface, and improves the ECL performance and stability of Ru@HKUST-1/Pd in aqueous solution. Furthermore, Au/Ni-Co NCs exhibited excellent quenching ability in this ECL system. HIL/Ru@HKUST-1/Pd as a luminophor and Au/Ni-Co NCs as a quencher were utilized to construct a sensor for the ZEN analysis. Under the optimized experimental parameters, the limit of detection of ZEN was determined to be 20 fg/mL (S/N = 3). This detection method addresses the issues of high cost and low sensitivity of existing methods, thereby ensuring food safety and the economic well-being of the livestock industry.

玉米赤霉烯酮（ZEN）主要由禾谷镰刀菌产生。这种霉菌毒素也被列入世界卫生组织国际癌症研究机构发布的致癌物清单。ZEN的主要作用器官是雌性动物的生殖系统，但它也对雄性动物产生特定的影响。因此，迫切需要对这种毒素进行灵敏的检测。本研究将Ru(bpy)32+和三正丙胺（TPrA）自组装到疏水离子液体（HIL）功能化的HKUST-1的孔隙中，从而制备了超灵敏电化学发光（ECL）微反应器来检测赤霉烯酮（ZEN）。特别是，HIL的引入使HKUST-1的亲水性表面变为疏水性表面，提高了Ru@HKUST-1/Pd在水溶液中的ECL性能和稳定性。此外，Au/Ni-Co NCs在ECL体系中表现出优异的淬灭能力。利用HIL/Ru@HKUST-1/Pd作为发光二极管，Au/Ni-Co nc作为淬灭剂构建ZEN分析传感器。在优化的实验参数下，ZEN的检出限为20 fg/mL （S/N = 3）。这种检测方法解决了现有方法成本高、灵敏度低的问题，从而确保了食品安全和畜牧业的经济福祉。

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引用次数: 0

A split-type photoelectrochemical biosensor using GSH-loaded liposomes in situ stimulation of ATA photoelectrode for the sensitive detection of AFP 一种采用gsh负载脂质体原位刺激ATA光电极的分体式光电化学生物传感器，用于AFP的灵敏检测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-21 DOI: 10.1016/j.bios.2025.118257

Min Li , Biao Zhao , Zuxing Zhang , Yu Li , Yixiao Li , Yingxian Xue , Shengbo Sang , Aoqun Jian

A non-enzymatic signal-amplified photoelectrochemical (PEC) was developed for the highly sensitive detection of alpha-fetoprotein (AFP) by employing glutathione-loaded liposomes (GSH@Lip) and AuNPs/TiO₂/Au (ATA) photoelectrodes. The ATA composite integrated well-designed optical trapping structure and to excite multiple light absorption via modes such as localized surface plasmon resonance (LSPR) and magnetic resonance mode. Furthermore, the signal was amplified using the high-loaded capacity of liposomes and the highly efficient GSH molecules to enhance the photocurrent of the ATA photoelectrodes. Specifically, AFP-specific recognition followed by centrifugation enriched with a large number of glutathione-encapsulated liposomes, whereas GSH can serve as an electron donor to scavenge the hole and inhibit the charge recombination. The incorporation of GSH-loaded liposomes into photoelectrochemical (PEC) immunoassays provides an enzyme-free alternative, eliminating the need for natural enzymes (e.g., HRP or ALP) while offering superior stability and simplified operation compared to conventional enzyme-based methods. The developed split-type PEC biosensor demonstrated outstanding analytical performance for AFP within the concentration range of 10⁻³ to 10³ ng mL⁻¹, achieving a low limit of detection (LOD) of 0.86 pg mL⁻¹, this covers the clinically relevant concentration range of AFP in patients with HCC (20–400 ng mL⁻¹). Noteworthy, for its stability, broad detection range, and rapid response, this biosensor presents an effective approach for cancer marker detection.

采用谷胱甘肽负载脂质体（GSH@Lip）和AuNPs/TiO2/Au （ATA）光电极，建立了一种用于甲胎蛋白（AFP）高灵敏度检测的非酶促信号放大光电化学（PEC）方法。ATA复合材料集成了设计良好的光捕获结构，并通过局域表面等离子体共振（LSPR）和磁共振模式激发多种光吸收。此外，利用脂质体的高负载能力和高效GSH分子增强ATA光电极的光电流，可以放大信号。具体来说，afp特异性识别之后是离心富集大量谷胱甘肽包封脂质体，而谷胱甘肽可以作为电子供体清除空穴并抑制电荷重组。将gsh负载脂质体结合到光电化学（PEC）免疫测定中提供了一种无酶的替代方法，消除了对天然酶（例如HRP或ALP）的需求，同时与传统的基于酶的方法相比，具有优越的稳定性和简化的操作。所开发的分体式PEC生物传感器在10−3 ~ 103 ng mL−1的浓度范围内具有出色的AFP分析性能，低检出限（LOD）为0.86 pg mL−1，涵盖HCC患者AFP临床相关浓度范围（20 ~ 400 ng mL−1）。值得注意的是，该生物传感器具有稳定性好、检测范围广、反应速度快等特点，为癌症标志物检测提供了有效途径。

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引用次数: 0

Target-inhibited MCOF-Apt@Ag self-assembly for multi-modal biosensor for detecting Vibrio parahaemolyticus 目标抑制MCOF-Apt@Ag自组装用于检测副溶血性弧菌的多模态生物传感器。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-21 DOI: 10.1016/j.bios.2025.118248

Jingyi Xiao , Tongtong Li , Jianing Sun, Xue Gao

This study proposed a colorimetric/fluorescence/SERS multi-modal biosensor based on aptamer-modified silver nanozymes (Ag@Apt) and magnetic covalent organic frameworks (MCOF) for the detection of V. parahaemolyticus. MCOF-Apt@Ag could catalyze the oxidation of the aggregation-induced emission luminogen tetra-(4-aminophenyl) ethylene (TPE-4A) to produce green TPE-4A²⁺. With the increase of V. parahaemolyticus, Ag@Apt preferentially bound to the bacteria, and the production of MCOF-Apt@Ag was suppressed, leading to a reduction in the enzymatic activity of the sensing system. TPE-4A²⁺ generated via catalytic oxidation decreased, leading to a color change of the system from dark green to light green. The UV–vis absorption at 600 nm decreased, while the fluorescence peak intensity of TPE-4A at 500 nm increased, thereby enabling both colorimetric and fluorescence detection. Additionally, silver nanozymes (Ag NPs) serve as SERS substrates and 4-mercaptobenzoic acid (4-MBA) acts as a SERS signal molecule. As the concentration of V. parahaemolyticus increased, the concentration of the Ag@Apt in the system also increased resulting in enhanced SERS signals from the biosensor. The lowest detection limits for colorimetric, fluorescence and SERS modes were 1.50 CFU/mL, 1.02 CFU/mL, and 0.97 CFU/mL. Multi-modal biosensors can provide stable multi-readout signals to ensure high detection sensitivity and accuracy, and a variety of quantitative modes with different detection capabilities can also improve the sensing performance.

本研究提出了一种基于适配体修饰银纳米酶（Ag@Apt）和磁性共价有机框架（MCOF）的比色/荧光/SERS多模态生物传感器，用于检测副溶血性弧菌。MCOF-Apt@Ag可以催化聚集诱导发射发光材料四-（4-氨基苯基）乙烯（TPE-4A）氧化生成绿色TPE-4A2+。随着副溶血性弧菌的增加，Ag@Apt优先与细菌结合，MCOF-Apt@Ag的产生受到抑制，导致感应系统的酶活性降低。催化氧化生成的TPE-4A2+减少，导致体系颜色由深绿色变为浅绿色。TPE-4A在600 nm处的紫外-可见吸收降低，而在500 nm处的荧光峰强度增加，从而可以同时进行比色和荧光检测。此外，银纳米酶（Ag NPs）作为SERS底物，4-巯基苯甲酸（4-MBA）作为SERS信号分子。随着副溶血性弧菌浓度的增加，系统中Ag@Apt的浓度也随之增加，导致来自生物传感器的SERS信号增强。比色、荧光和SERS模式的最低检出限分别为1.50 CFU/mL、1.02 CFU/mL和0.97 CFU/mL。多模态生物传感器可以提供稳定的多读出信号，保证较高的检测灵敏度和精度，多种具有不同检测能力的定量模式也可以提高传感性能。

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引用次数: 0

Advanced surface-functionalized optical fiber biosensing platform for ultrasensitive fructose quantification in biological fluids 用于生物液体中超灵敏果糖定量的先进表面功能化光纤生物传感平台。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-21 DOI: 10.1016/j.bios.2025.118258

Lan Yang , Yansong Li , Ruiduo Wang , Tongyuan Kang , Depeng Kong , Jianshe Li , Man Jiang , Hailiang Du , Pingyi Song , Yaomin Zhu

The human blood's concentration evaluation of fructose demonstrates significant clinical importance due to that it is closely associated with various diseases. However, current clinical technologies face challenges in achieving rapid quantitative analysis of fructose. The cascaded fiber sensor was proposed by fabricating microsphere cavities through the sequential fusion of single-mode fibers and seven-core fiber, followed by flame tapering of the seven-core fiber. Through optimization of the tapering length parameters, a structure with high refractive index sensitivity was obtained. By chemically immobilizing ketohexokinase (KHK) onto the fiber sensor surface, we established a specific biological functional layer that provides targeted binding sites. Spectral measurements of fructose standards at gradient concentrations demonstrated sensing sensitivity of 5.98 nm/(μg/mL) and detection limit of 12.6 ng/mL. Subsequent measurements of fructose solutions at gradient concentrations in human serum samples revealed that the sensor exhibits strong specificity and anti-interference capabilities in serum. Moreover, we performed the Bland-Altman analysis by comparing the quantitative analysis results of fiber sensor with liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS) method. The analysis confirmed the validity and accuracy of the sensor's detection capability towards clinically unknown serum samples. This method offers several advantages, including minimal sample volume requirements, label-free, rapid analysis and low cost, demonstrating significant potential for clinical applications in fructose detection.

果糖与多种疾病密切相关，因此人体血液中果糖的浓度评价具有重要的临床意义。然而，目前的临床技术在实现果糖的快速定量分析方面面临挑战。通过单模光纤与七芯光纤的顺序融合，制备微球腔，然后将七芯光纤的火焰逐渐变细，提出了级联光纤传感器。通过对锥形长度参数的优化，得到了具有高折射率灵敏度的结构。通过化学固定酮己糖激酶（KHK）到纤维传感器表面，我们建立了一个特定的生物功能层，提供靶向结合位点。梯度浓度下果糖标准品的光谱测量结果显示，检测灵敏度为5.98 nm/(μg/mL)，检出限为12.6 ng/mL。随后对人血清样品中梯度浓度的果糖溶液的测量表明，该传感器在血清中具有很强的特异性和抗干扰能力。此外，我们将光纤传感器的定量分析结果与液相色谱-三重四极杆质谱（LC-MS）方法进行了Bland-Altman分析。分析证实了传感器对临床未知血清样本检测能力的有效性和准确性。该方法具有样品体积要求小、无标签、快速分析和低成本等优点，在果糖检测方面具有巨大的临床应用潜力。

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引用次数: 0

Cellular metabolism biosensing: From extracellular microenvironment to intracellular physiology monitoring 细胞代谢生物传感：从细胞外微环境到细胞内生理监测

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-21 DOI: 10.1016/j.bios.2025.118250

Tao Liang , Chenlei Gu , Jiaru Fang , Dongxin Xu , Zhen Wang , Ciro Chiappini , Ning Hu

Metabolism is crucial for the growth, development, and life activities of organisms. Microsensors designed to monitor cellular metabolism are essential for investigation in cell culture. Electrochemical and optical sensors are dominantly applied to analyze classic metabolites in extracellular microenvironments, such as protons, oxygen, glucose, pyruvate, lactate, and reactive oxygen/nitrogen species. These methodologies facilitate the label-free, noninvasive, and continuous recording of transient effects, providing insights beyond static images and endpoint assays. This comprehensive review aims to critically evaluate and discuss advances in cellular metabolic monitoring, particularly focusing on the utilization of integrated biosensors in cell culture and detection systems, which are commonly termed as microphysiological systems. Initially, metabolic pathways are analyzed to elucidate the roles of key metabolites related to acidification, respiration, energy metabolism, and transient reactive species. Subsequently, this review comprehensively discusses sensing analytes, mechanisms, systems, cultures, and applications in advanced multimodal biosensing systems, including commercial products, as documented in references. Finally, current challenges and future directions associated with the development of more advanced metabolic biosensing systems are delineated to enhance their sensing capabilities, and meet the emerging clinical and scientific demands.

新陈代谢对生物体的生长、发育和生命活动至关重要。用于监测细胞代谢的微传感器在细胞培养研究中是必不可少的。电化学和光学传感器主要用于分析细胞外微环境中的经典代谢物，如质子、氧、葡萄糖、丙酮酸盐、乳酸盐和活性氧/氮。这些方法促进了无标签、无创和连续记录瞬态效应，提供了超越静态图像和终点分析的见解。这篇全面的综述旨在批判性地评估和讨论细胞代谢监测的进展，特别是关注集成生物传感器在细胞培养和检测系统中的应用，这些系统通常被称为微生理系统。首先，对代谢途径进行分析，阐明与酸化、呼吸、能量代谢和瞬时反应物质相关的关键代谢物的作用。随后，本文全面讨论了传感分析物、机制、系统、培养以及在先进的多模态生物传感系统中的应用，包括参考文献中记录的商业产品。最后，描述了当前的挑战和未来与更先进的代谢生物传感系统发展相关的方向，以增强其传感能力，满足新兴的临床和科学需求。

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引用次数: 0

Highly integrated chip for continuous chemical synthesis, purification and inline biosensoric monitoring of biological activity on stem cell-derived 3D cardiomyocyte cultures 高度集成的芯片，用于连续化学合成，纯化和在线生物传感器监测干细胞衍生的3D心肌细胞培养物的生物活性

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-21 DOI: 10.1016/j.bios.2025.118256

T. Haensch , F.D. Zitzmann , S. Schmidt , C. Stanko , E. Paternoga , M. Meier , K. Zeitler , D. Belder , A.A. Robitzki , H.-G. Jahnke

Miniaturization and development of lab-on-a-chip (LOC) devices are a fast-evolving research field. While single processes e.g. pharmacological compound testing on cells and organoids, diagnostic applications, chemical synthesis or on-chip analytics are widely developed and in use, the integration of multi-step processes such as chemical synthesis, product separation and purification in combination with biological activity monitoring of synthesis products in compact LOC devices is poorly realized. Especially the integration of label-free, biosensoric analysis techniques is still a bottleneck.

In this context, we developed an integrated microfluidic chip that combines an organic on-chip synthesis with subsequent continuous product purification and solvent-exchange by micro free flow electrophoresis (μFFE), followed by re-buffering to physiological conditions and finally, the biosensoric detection of biological activity on 3D cultures. As a proof of concept, the synthesis of the cardioactive drug propranolol from its precursor was chosen and successfully established in a continuous microfluidic setup. For sensitive detection of bioactivity, human stem cell-derived cardiomyocyte 3D cultures were used. The integration of microcavity microelectrode arrays, combined with impedance spectroscopy enabled label-free, non-invasive, real-time monitoring of propranolol isolation and purification as well as the quantification of the negative chronotropic effect of propranolol. The organoids show the typical negative chronotropic effect of propranolol in the μM range. Taken together, the entire process chain including chemical synthesis, purification, and inline monitoring of biological activity on a single LOC device, was successfully demonstrated.

芯片实验室（lab-on-a-chip， LOC）器件的小型化和发展是一个快速发展的研究领域。虽然在细胞和类器官上的药理化合物测试、诊断应用、化学合成或芯片上分析等单一过程得到了广泛的开发和使用，但将化学合成、产品分离和纯化等多步骤过程与紧凑型LOC设备中合成产品的生物活性监测相结合的集成实现得很差。特别是整合无标签，生物传感分析技术仍然是一个瓶颈。在此背景下，我们开发了一种集成微流控芯片，将有机片上合成与随后的连续产物纯化和微自由流动电泳（μFFE）的溶剂交换结合起来，然后再缓冲到生理条件，最后在3D培养物上进行生物活性的生物传感检测。作为概念的证明，选择了从其前体合成心脏活性药物心得安，并在连续微流体装置中成功建立。为了灵敏地检测生物活性，使用人干细胞衍生的心肌细胞3D培养物。集成微腔微电极阵列，结合阻抗谱技术，实现了无标签、无创、实时监测普萘洛尔的分离纯化，并定量分析了普萘洛尔的负变时效应。在μM范围内，类器官表现出典型的负变时效应。综上所述，整个过程链包括化学合成、净化和生物活性在线监测在一个单一的LOC设备上，成功地展示。

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引用次数: 0

Dual-mode sensor array based on silica-coated carbon dots with tunable fluorescence and phosphorescence for discrimination and detection of monoamine neurotransmitters 基于硅包覆碳点的荧光和磷光可调双模传感器阵列，用于单胺类神经递质的识别和检测。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-20 DOI: 10.1016/j.bios.2025.118224

Yongbo Wang , Yuanna Ning , Yanting Jia , Shaojie Wang , Wencai Wang , Chengyuan Liang , Yingkun Ren

Detection and discrimination of monoamine neurotransmitters (MNTs) are critical for early diagnosis but remain a substantial challenge due to their similar molecular structures and the complex interferences from biological sample matrices. Herein, dual-mode (fluorescence/phosphorescence) sensor arrays based on silica-coated carbon dots (CDs@SiO₂) were developed for discrimination and detection of multiple MNTs. The synthesized CDs@SiO₂ displayed dual fluorescence (395 nm) and phosphorescence (520 nm) emission, enabling a one-component dual-mode sensor array for discrimination of catecholamines (100 % accuracy). The CDs-RhB@SiO₂ nanocomposites, prepared by co-confining CDs and rhodamine B (RhB) in silica, exhibited orange fluorescence (585 nm) and red phosphorescence (600 nm). This structure enables efficient FRET (77.4 %) from CDs to RhB, yielding long-wavelength phosphorescence. Subsequently, a two-component four-channel sensor array utilizing CDs@SiO₂ and CDs-RhB@SiO₂ was developed. By leveraging distinct fluorescence (λ_em = 395/585 nm) and phosphorescence (λ_em = 520/600 nm) signals, the sensor array achieved precise discrimination (100 % accuracy) and quantitative detection of multiple MNTs with a low detection limit of 1.25 μM. This work provides a strategy to develop multimode sensor arrays with low background interference, demonstrating promise as a proof-of-concept platform that may support early diagnostic applications.

单胺类神经递质（MNTs）的检测和鉴别对于早期诊断至关重要，但由于其相似的分子结构和来自生物样品基质的复杂干扰，仍然是一个重大挑战。本文开发了基于二氧化硅涂层碳点（CDs@SiO2）的双模（荧光/磷光）传感器阵列，用于识别和检测多个mnt。合成的CDs@SiO2显示出双荧光（395 nm）和磷光（520 nm）发射，使单组分双模传感器阵列能够识别儿茶酚胺（100%准确）。将CDs和罗丹明B （RhB）共包埋在二氧化硅中制备的CDs-RhB@SiO2纳米复合材料显示出橙色荧光（585 nm）和红色磷光（600 nm）。这种结构可以实现从CDs到RhB的高效FRET(77.4%)，产生长波长的磷光。随后，开发了一种利用CDs@SiO2和CDs-RhB@SiO2的双分量四通道传感器阵列。该传感器阵列利用不同的荧光（λem = 395/585 nm）和磷光（λem = 520/600 nm）信号，实现了对多个mnt的精确识别（100%准确率）和定量检测，检测限低至1.25 μM。这项工作为开发具有低背景干扰的多模传感器阵列提供了一种策略，证明了作为一个概念验证平台可能支持早期诊断应用的前景。

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引用次数: 0

A digital SERS platform powered by bispecific antibody fragments for SARS-CoV-2 diagnostics 由双特异性抗体片段驱动的数字SERS平台，用于SARS-CoV-2诊断。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-20 DOI: 10.1016/j.bios.2025.118252

Jing Wang , Quan Zhou , Kym Lowry , Christopher B. Howard , Matt Trau

Surface-enhanced Raman scattering (SERS) immunoassays are powerful analytical tools for protein detection while typically rely on the availability of suitable antibodies. Compared to full-length antibodies, antibody fragments provide advantages such as rapid and cost-effective production. However, oriented conjugation of antibody fragments to SERS nanotags, essential for maintaining their functionality, remains relatively underexplored. Here, we introduce a bispecific antibody (BsAb)-programmable digital immunochemistry detection regime implemented on an advanced digital SERS platform (“DigibiSERS”), enabling deterministic molecular orientation, variance‐robust single‐particle event calling, and kinetic regularization within one unified platform. Specifically, we fuse an anti-nucleocapsid nanobody with a single-chain variable fragment targeting methoxy polyethylene glycol (mPEG) grafted onto the SERS nanotag surfaces. This design facilitates straightforward, oriented BsAb conjugation, preserving its functionality. The DigibiSERS platform, incorporating single-particle active SERS nanotags, nanomixing-enhanced microchips, and digital readouts, demonstrates high sensitivity and specificity, achieving detection limits of 2.01 ng/mL for nucleocapsid protein and 2.7 copies/mL for virus. An area under the curve (AUC) of 0.8783 highlights the potential of engineered antibody fragments in enhancing the clinical sensitivity and practicality of SERS-based immunoassays for infectious disease diagnostics.

表面增强拉曼散射（SERS）免疫测定是蛋白质检测的强大分析工具，但通常依赖于合适抗体的可用性。与全长抗体相比，抗体片段具有生产速度快、成本低等优点。然而，抗体片段与SERS纳米标签的定向偶联，对于维持其功能至关重要，仍然相对缺乏探索。在这里，我们引入了一种双特异性抗体(BsAb)-可编程数字免疫化学检测方案，该方案在先进的数字SERS平台（“DigibiSERS”）上实现，在一个统一的平台内实现确定性分子取向，方差鲁棒性单粒子事件调用和动力学正则化。具体来说，我们将抗核衣壳纳米体与靶向甲氧基聚乙二醇（mPEG）的单链可变片段融合到SERS纳米标签表面。这种设计有利于直接的，定向的BsAb共轭，保留其功能。DigibiSERS平台结合了单颗粒活性SERS纳米标签、纳米混合增强微芯片和数字读数，具有高灵敏度和特异性，对核衣壳蛋白的检测限为2.01 ng/mL，对病毒的检测限为2.7 copies/mL。曲线下面积（AUC）为0.8783，表明工程化抗体片段在提高基于sers的传染病诊断免疫测定的临床敏感性和实用性方面具有潜力。

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引用次数: 0

Perovskite–COF hybrid enables sequence-programmed, anti-correlated ECL/SERS readout of NF-κB p50 钙钛矿- cof杂化实现了序列编程、抗相关的NF-κB p50的ECL/SERS读数。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-20 DOI: 10.1016/j.bios.2025.118253

Yanfei Ma , Chan Yang , Fengqing Liao , Suai Deng , Qianli Tang , Kai Zhang , Xianjiu Liao

Physical medicine and rehabilitation (PM&R) needs mechanism-anchored biomarkers that change early enough to guide exercise dosing and physiotherapy. Here we position NF-κB p50 as a PM&R biomarker—read out not by bulk abundance but by DNA-binding activity that reflects the inflammation-to-repair transition. We develop a dual-mode ratiometric sensor in which the same p50–κB recognition event yields opposite signals—electrochemiluminescence (ECL) increase and SERS decrease—combined as R=I_ECL/I_SERS to self-normalize interface and matrix variability. The platform shows ultrasensitive detection (attomolar-level), wide working range, high specificity versus related nuclear proteins, and strong robustness across batches and complex matrices (cell lysate, spiked plasma) with ≤100 μL sample. By providing a pathway-activity readout, p50 complements routine clinical markers (e.g., CRP, IL-6, NT-proBNP) and links molecular response to functional recovery. We outline PM&R use cases—musculoskeletal repair, osteoarthritis rehabilitation, and neurorehabilitation—where serial R trajectories can inform progression/overload decisions and personalize therapy. This work frames p50 detection as a practical, translational biomarker for PM&R/physiotherapy, and offers a robust sensing architecture readily extendable to other rehabilitation-relevant transcriptional pathways.

物理医学和康复（PM&R）需要机制锚定的生物标志物，这些标志物可以在足够早的时候改变，以指导运动剂量和物理治疗。在这里，我们将NF-κB p50定位为PM&R生物标志物-不是通过大量丰度而是通过反映炎症到修复转变的dna结合活性来解读。我们开发了一种双模比例传感器，其中相同的p50-κB识别事件产生相反的信号-电化学发光（ECL）增加和SERS降低-结合R=IECL/ISERS来自归一化界面和矩阵变异性。该平台检测灵敏度高（原子摩尔级），工作范围宽，对相关核蛋白具有高特异性，在≤100 μL样品的批次和复杂基质（细胞裂解液、加标血浆）中具有较强的稳健性。通过提供通路活性读数，p50补充了常规临床标志物（如CRP、IL-6、NT-proBNP），并将分子反应与功能恢复联系起来。我们概述了PM&R用例-肌肉骨骼修复，骨关节炎康复和神经康复-其中系列R轨迹可以为进展/过载决策和个性化治疗提供信息。这项工作将p50检测作为PM&R/物理治疗的实用、可翻译的生物标志物，并提供了一个强大的传感架构，易于扩展到其他康复相关的转录途径。

{"title":"Perovskite–COF hybrid enables sequence-programmed, anti-correlated ECL/SERS readout of NF-κB p50","authors":"Yanfei Ma , Chan Yang , Fengqing Liao , Suai Deng , Qianli Tang , Kai Zhang , Xianjiu Liao","doi":"10.1016/j.bios.2025.118253","DOIUrl":"10.1016/j.bios.2025.118253","url":null,"abstract":"<div><div>Physical medicine and rehabilitation (PM&R) needs mechanism-anchored biomarkers that change early enough to guide exercise dosing and physiotherapy. Here we position NF-κB p50 as a PM&R biomarker—read out not by bulk abundance but by DNA-binding activity that reflects the inflammation-to-repair transition. We develop a dual-mode ratiometric sensor in which the same p50–κB recognition event yields opposite signals—electrochemiluminescence (ECL) increase and SERS decrease—combined as R=I<sub>ECL</sub>/I<sub>SERS</sub> to self-normalize interface and matrix variability. The platform shows ultrasensitive detection (attomolar-level), wide working range, high specificity versus related nuclear proteins, and strong robustness across batches and complex matrices (cell lysate, spiked plasma) with ≤100 μL sample. By providing a pathway-activity readout, p50 complements routine clinical markers (e.g., CRP, IL-6, NT-proBNP) and links molecular response to functional recovery. We outline PM&R use cases—musculoskeletal repair, osteoarthritis rehabilitation, and neurorehabilitation—where serial R trajectories can inform progression/overload decisions and personalize therapy. This work frames p50 detection as a practical, translational biomarker for PM&R/physiotherapy, and offers a robust sensing architecture readily extendable to other rehabilitation-relevant transcriptional pathways.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"294 ","pages":"Article 118253"},"PeriodicalIF":10.5,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145595509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-channel electrochemical biosensor for simultaneous monitoring of glucose and uric acid 用于同时监测葡萄糖和尿酸的单通道电化学生物传感器。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-11-20 DOI: 10.1016/j.bios.2025.118255

Changyun Quan , Liping Wen , Minghui Yang , Binjie Xu

Given the metabolic interplay between glucose and uric acid, individuals should proactively monitor and be alert of elevated levels of both substances. For the first time, this paper describes a novel design of electrochemical biosensor based on screen-printed electrode of a single channel but capable of simultaneously measuring blood glucose and uric acid concentrations. Experimental results demonstrated that this single-channel biosensor exhibits good performance towards glucose and uric acid, such as broad linear range, good reproducibility, good stability, and strong resistance against interference. For spiked recovery experiments on human venous blood samples, the glucose recovery rate ranged from 95.9 % to 103.2 %, while the uric acid recovery rate ranged from 99.7 % to 104.9 %. And the relative standard deviation for both parameters was less than 5 %, indicating that this biosensor holds promising prospects for commercial application. This is truly the first time that enable simultaneous detection of glucose and uric acid concentrations using just one drop of blood and a single test strip. It reduces the number of blood draws required, lowers analysis costs, and significantly improves screening efficiency for individuals with diabetes and hyperuricemia.

鉴于葡萄糖和尿酸之间的代谢相互作用，个人应主动监测和警惕这两种物质的水平升高。本文首次提出了一种基于单通道丝网印刷电极的电化学生物传感器，可同时测量血糖和尿酸浓度。实验结果表明，该单通道生物传感器对葡萄糖和尿酸具有线性范围宽、重现性好、稳定性好、抗干扰能力强等良好性能。对人静脉血样品进行加标回收率实验，葡萄糖回收率为95.9% ~ 103.2%，尿酸回收率为99.7% ~ 104.9%。两个参数的相对标准偏差均小于5%，表明该生物传感器具有良好的商业应用前景。这确实是第一次使用一滴血和一条试纸同时检测葡萄糖和尿酸浓度。它减少了所需的抽血次数，降低了分析成本，并显著提高了糖尿病和高尿酸血症患者的筛查效率。

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引用次数: 0